RchiOBHm_Chr1g0368331

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0011819
Physical Location & Seq
Reverse (-)
58500375 .. 58505175
4801 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2049 bp
ATGGGATTTTTCGGGAATGGCGATTGGTTGAGGATCCCATTGATCATCAAAATATGGGGGTTCATCATGATCTCCGTCACCTTCTTCTACTTGGGCAAGAACTGGACCGGTGGGGCCCAGCAGCTCCTCTCCTTCACCACCGCCGCCACCACGCGCCGATCTCCTCCTTCCGCCACCGTCTCCATTTCCCCCAATCTCAACAACCCCTTCAACCTCTCCGCCCTCCTCAACGAAACCCTGGCCCCGCCGCCCTTGCCTTCTCCTCCTCCGCCTCCGCCGCCGCTTCCTTCTTCTCCGCCGCCCCCCGCGCCGCCGCCGTCGGACGTGATCCAGCGGTTCGGGATCGTGGACGAGAACGGGACCATGGCCGACGATTTCGAGGTCGGCGAGTTCGATCCCGATGAGGTGGTGGAGAATTGGAGCGGCCAGAATGGGACGGCGGCGGCGGCGGAGAGTGCCGGGAGCGGGTCGGGTAGGTTCAATGGGAAGAGGTTTGAGCTTTGTCGTAACGGTATGAGCGAGTACATACCGTGTTTGGATAATGTGGTGGCCATTAAGAGGCTCAAATCCACCGATAAAGGCGAGCGCTTTGAGCGGCATTGTCCGGATTCCGGCAAGGGTTTGAATTGCTTGGTTCCGCCGCCCAAGAACTACAAGAGCCCCATTCCATGGCCGAAGAGCCGCGATGAGGTTTGGTTCAATAATGTACCTCATACTCGTCTGGTTGAGGATAAAGGGGGTCAGAACTGGATTACACGAGAAAAGAAGAAGTTTAAGTTTCCGGGAGGTGGGACACAGTTTATACATGGGGCAAATGAGTACTTGGATCATATTTCTAAGATGATCCCTGACATCACATTTGGTCACCATATTCGAGTTGCATTGGATGTTGGATGTGGTGTTGCAAGTTTTGGTGCATACCTGCTGCAACGGAATGTCATCACTATGTCAATTGCTCCAAAAGATGTCCATGAGAATCAGATACAATTTGCTCTTGAGCGTGGAGTGCCTGCAATGGTAGCAGCATTTGCTACTCGCCGTTTATTGTACCCAAGTCAGGCATTTGACTTGATCCATTGCTCACGATGCAGGATTAATTGGACTCGTGACGATGGGATATTGCTACTTGAGGTTAATAGGATGCTCCGGGCAGGAGGATACTTTGTTTGGGCAGCACAGCCAGTTTATAAGCATGAAGAAGTCCTAGAGGAGCAGTGGCAAGAGATGCTTAACCTCACGACCCGTCTTTGCTGGGAGTTTGTGAAAAAGGATGGCTATGTTGCCGTATGGCAGAAGCCAGTTAACAACAGCTGCTATCTAGGTCGGGATGCTGAAACCAAACCTCCATTATGCGACTCAAGTGATGACCCGGACAAAGTTTGGTATGTTGATCTGAAGGCATGCATAACAAGACTCCCTGAAAATGGTTATGGAGCAAATGTCACAACATGGCCAGACCGCATGCAAACTCCCCCTGATAGGCTTCAGAGCATACAGTTTGATGCCTATATATCCAGAAAGGAGCTCTTCAAGGCAGAATCTAAATATTGGCATGAAATAATTGAAGGCTATGTCCGTGCTTTTAAATGGAAGAAGTTAAGATTAAGAAATGTAATGGATATGAGAGCTGGCTTTGGAGGATTTGCAGTGGCACTAATGGAACATAATTTAAATTCCTGGGTTATGAATGTGGTTCCTGTTAGCGGCCCCAACACCTTGCCTGTTATATATGACCGTGGATTTATTGGAGTTATGCATGACTGGTGTGAGCCATTTGATACATACCCAAGAACCTATGATTTATTACATGCAGCTGGCCTCTTCTCTATTGAGAAGAAAAGGTGCAATATGTCAACAATCATGCTTGAGATGGACCGTATGCTAAGACCAGGTGGACGTGTATACATCCGTGATGCTCTTGCGGTCATGGATGAGCTTCAAGCGATTGGAAATGCCATGGGCTGGAAAGTTAATTTGCGAGACACATCTGAGGGTCCTCATGCAAGCTATAGGCTCTTCATGGCTGAAAAACGCCTCTTGCATAGTTGA

Protein Analysis

682

Amino Acids

77.02

Weight (kDa)

7.88

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 174 - 677 5.7e-232 Putative S-adenosyl-L-methionine-dependent methyltransferase
Methyltransf_11 PF08241 295 - 389 7.6e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011819)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G39750 AT5G06050
fragaria_vesca FvH4_7g23080 FvH4_7g23080
malus_domestica MD01G1139400.v1.1 MD07G1203800.v1.1
prunus_persica Prupe.2G239900_v2.0.a1
pyrus_communis pycom01g16000 pycom07g19040
rosa_chinensis RchiOBHm_Chr1g0368331
rosa_laevigata RLG00000027219
rosa_roxburghii Rroxscaffold_4G00288640
rosa_rugosa Rorug01G0341200 Rorug01G0341200
rosa_samantha Rh1AG347800 Rh1BG310800 Rh1CG326200 Rh1DG342600
rosa_wichuraiana Rw1G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1188
Acc36I ACCTGC 1 cut(s) 930
AccB7I CCANNNNNTGG 2 cut(s) 669, 1962
AccBSI CCGCTC 3 cut(s) 423, 465, 595
AccI GTMKAC 1 cut(s) 1902
AccII CGCG 3 cut(s) 154, 308, 684
AccIII TCCGGA 1 cut(s) 604
AclWI GGATC 8 cut(s) 28, 41, 322, 350, 389, 834, 838, 1066
AcoI YGGCCR 5 cut(s) 366, 424, 549, 671, 1451
AcsI RAATTY 1 cut(s) 1672
AcuI CTGAAG 2 cut(s) 1415, 1469
AfaI GTAC 4 cut(s) 524, 708, 821, 1049
AfeI AGCGCT 1 cut(s) 587
AflIII ACRYGT 1 cut(s) 1897
AgeI ACCGGT 1 cut(s) 107
AgsI TTSAA 7 cut(s) 211, 481, 625, 700, 1531, 1565, 1940
AjiI CACGTC 2 cut(s) 325, 1898
AjnI CCWGG 3 cut(s) 237, 1676, 1888
AluBI AGCT 8 cut(s) 124, 499, 1311, 1525, 1628, 1814, 1936, 2007
AluI AGCT 8 cut(s) 124, 499, 1311, 1525, 1628, 1814, 1936, 2007
Alw21I GWGCWC 1 cut(s) 1527
Alw26I GTCTC 2 cut(s) 184, 1974
AlwI GGATC 8 cut(s) 28, 41, 322, 350, 389, 834, 838, 1066
Aor13HI TCCGGA 1 cut(s) 604
Aor51HI AGCGCT 1 cut(s) 587
AoxI GGCC 9 cut(s) 114, 240, 366, 424, 549, 671, 1451, 1705, 1816
ApaI GGGCCC 1 cut(s) 118
ApeKI GCWGC 6 cut(s) 121, 925, 1022, 1172, 1311, 1811
ApoI RAATTY 1 cut(s) 1672
ArsI GACNNNNNNTTYG 2 cut(s) 842, 874
AseI ATTAAT 1 cut(s) 1095
AsiGI ACCGGT 1 cut(s) 107
AspLEI GCGC 3 cut(s) 156, 310, 588
AspS9I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1706, 1873, 1994
AsuC2I CCSGG 4 cut(s) 460, 783, 1148, 1370
AsuHPI GGTGA 3 cut(s) 70, 127, 857
AvaII GGWCC 4 cut(s) 105, 360, 1873, 1994
BaeGI GKGCMC 1 cut(s) 118
BalI TGGCCA 2 cut(s) 551, 1453
BamHI GGATCC 1 cut(s) 33
BanII GRGCYC 3 cut(s) 118, 662, 1527
BauI CACGAG 2 cut(s) 756, 1104
Bbv12I GWGCWC 1 cut(s) 1527
BbvI GCAGC 6 cut(s) 133, 912, 1034, 1184, 1298, 1823
BccI CCATC 3 cut(s) 1106, 1265, 1864
BceAI ACGGC 4 cut(s) 301, 453, 1023, 1268
BciT130I CCWGG 3 cut(s) 239, 1678, 1890
BciVI GTATCC 1 cut(s) 1151
BclI TGATCA 1 cut(s) 42
BcnI CCSGG 4 cut(s) 460, 783, 1148, 1370
BcoDI GTCTC 2 cut(s) 184, 1974
BfaI CTAG 2 cut(s) 1205, 1319
BfmI CTRYAG 1 cut(s) 2008
BfoI RGCGCY 1 cut(s) 589
BfuAI ACCTGC 1 cut(s) 930
BfuI GTATCC 1 cut(s) 1151
BmcAI AGTACT 1 cut(s) 821
Bme1390I CCNGG 7 cut(s) 239, 460, 783, 1148, 1370, 1678, 1890
Bme18I GGWCC 4 cut(s) 105, 360, 1873, 1994
BmgBI CACGTC 2 cut(s) 325, 1898
BmgT120I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1706, 1873, 1994
BmiI GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 636, 1695, 1708, 1995
BmrFI CCNGG 7 cut(s) 239, 460, 783, 1148, 1370, 1678, 1890
BmsI GCATC 6 cut(s) 1076, 1131, 1215, 1318, 1492, 1903
BpuEI CTTGAG 4 cut(s) 1016, 1148, 1342, 1886
BpuMI CCSGG 4 cut(s) 460, 783, 1148, 1370
BsaJI CCNNGG 6 cut(s) 237, 363, 668, 1677, 1735, 1956
BsaWI WCCGGW 2 cut(s) 107, 604
BsaXI ACNNNNNCTCC 4 cut(s) 1327, 1357, 1425, 1455
Bse118I RCCGGY 1 cut(s) 107
Bse1I ACTGG 5 cut(s) 107, 752, 1181, 1298, 1766
Bse3DI GCAATG 2 cut(s) 1020, 1075
BseAI TCCGGA 1 cut(s) 604
BseBI CCWGG 3 cut(s) 239, 1678, 1890
BseDI CCNNGG 6 cut(s) 237, 363, 668, 1677, 1735, 1956
BseGI GGATG 7 cut(s) 892, 899, 1146, 1276, 1333, 1905, 1936
BseMI GCAATG 2 cut(s) 1020, 1075
BseMII CTCAG 1 cut(s) 1980
BseNI ACTGG 5 cut(s) 107, 752, 1181, 1298, 1766
BseRI GAGGAG 6 cut(s) 116, 153, 215, 252, 255, 1223
BseSI GKGCMC 1 cut(s) 118
BseXI GCAGC 6 cut(s) 133, 912, 1034, 1184, 1298, 1823
BseYI CCCAGC 2 cut(s) 117, 1251
Bsh1236I CGCG 3 cut(s) 154, 308, 684
BshFI GGCC 9 cut(s) 116, 242, 368, 426, 551, 673, 1453, 1707, 1818
BshTI ACCGGT 1 cut(s) 107
BsiHKAI GWGCWC 1 cut(s) 1527
BsiSI CCGG 7 cut(s) 108, 459, 605, 612, 782, 1147, 1370
BslFI GGGAC 3 cut(s) 373, 448, 805
BsmAI GTCTC 2 cut(s) 184, 1974
BsmBI CGTCTC 1 cut(s) 184
BsmFI GGGAC 3 cut(s) 373, 448, 805
BsnI GGCC 9 cut(s) 116, 242, 368, 426, 551, 673, 1453, 1707, 1818
Bsp120I GGGCCC 1 cut(s) 114
Bsp1286I GDGCHC 3 cut(s) 118, 662, 1527
Bsp13I TCCGGA 1 cut(s) 604
Bsp19I CCATGG 3 cut(s) 363, 668, 1956
BspANI GGCC 9 cut(s) 116, 242, 368, 426, 551, 673, 1453, 1707, 1818
BspCNI CTCAG 1 cut(s) 1981
BspEI TCCGGA 1 cut(s) 604
BspFNI CGCG 3 cut(s) 154, 308, 684
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 636, 1695, 1708, 1995
BspMI ACCTGC 1 cut(s) 930
BspPI GGATC 8 cut(s) 28, 41, 322, 350, 389, 834, 838, 1066
BspQI GCTCTTC 3 cut(s) 671, 1532, 2021
BsrBI CCGCTC 3 cut(s) 423, 465, 595
BsrDI GCAATG 2 cut(s) 1020, 1075
BsrFI RCCGGY 1 cut(s) 107
BsrI ACTGG 5 cut(s) 107, 752, 1181, 1298, 1766
BssAI RCCGGY 1 cut(s) 107
BssECI CCNNGG 6 cut(s) 237, 363, 668, 1677, 1735, 1956
BssNAI GTATAC 1 cut(s) 1903
BssSI CACGAG 2 cut(s) 756, 1104
BssT1I CCWWGG 3 cut(s) 363, 668, 1956
Bst1107I GTATAC 1 cut(s) 1903
Bst2BI CACGAG 2 cut(s) 756, 1104
Bst2UI CCWGG 3 cut(s) 239, 1678, 1890
Bst4CI ACNGT 7 cut(s) 178, 512, 531, 798, 1497, 1736, 1877
Bst6I CTCTTC 5 cut(s) 482, 671, 1532, 1826, 2021
BstAPI GCANNNNNTGC 2 cut(s) 1028, 1225
BstC8I GCNNGC 7 cut(s) 584, 1011, 1402, 1463, 1630, 1816, 2005
BstDEI CTNAG 3 cut(s) 837, 1883, 1989
BstDSI CCRYGG 4 cut(s) 363, 668, 1735, 1956
BstEII GGTNACC 1 cut(s) 863
BstF5I GGATG 7 cut(s) 892, 899, 1146, 1276, 1333, 1905, 1936
BstFNI CGCG 3 cut(s) 154, 308, 684
BstH2I RGCGCY 1 cut(s) 589
BstHHI GCGC 3 cut(s) 156, 310, 588
BstMAI GTCTC 2 cut(s) 184, 1974
BstNI CCWGG 3 cut(s) 239, 1678, 1890
BstNSI RCATGY 3 cut(s) 1404, 1465, 1811
BstPI GGTNACC 1 cut(s) 863
BstSCI CCNGG 7 cut(s) 237, 458, 781, 1146, 1368, 1676, 1888
BstSFI CTRYAG 1 cut(s) 2008
BstSLI GKGCMC 1 cut(s) 118
BstUI CGCG 3 cut(s) 154, 308, 684
BstV1I GCAGC 6 cut(s) 133, 912, 1034, 1184, 1298, 1823
BstX2I RGATCY 1 cut(s) 33
BstYI RGATCY 1 cut(s) 33
BstZ17I GTATAC 1 cut(s) 1903
BsuI GTATCC 1 cut(s) 1151
BsuRI GGCC 9 cut(s) 116, 242, 368, 426, 551, 673, 1453, 1707, 1818
BtgI CCRYGG 4 cut(s) 363, 668, 1735, 1956
BtgZI GCGATG 1 cut(s) 699
BtrI CACGTC 2 cut(s) 325, 1898
BtsCI GGATG 7 cut(s) 892, 899, 1146, 1276, 1333, 1905, 1936
BtsI GCAGTG 2 cut(s) 1220, 1653
BtsIMutI CAGTG 2 cut(s) 1220, 1653
BveI ACCTGC 1 cut(s) 930
Cac8I GCNNGC 7 cut(s) 584, 1011, 1402, 1463, 1630, 1816, 2005
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 156, 310, 588
Cfr10I RCCGGY 1 cut(s) 107
Cfr13I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1706, 1873, 1994
CsiI ACCWGGT 1 cut(s) 1888
Csp6I GTAC 4 cut(s) 523, 707, 820, 1048
CspAI ACCGGT 1 cut(s) 107
CviQI GTAC 4 cut(s) 523, 707, 820, 1048
DdeI CTNAG 3 cut(s) 837, 1883, 1989
DraI TTTAAA 2 cut(s) 1585, 1671
EaeI YGGCCR 5 cut(s) 366, 424, 549, 671, 1451
Eam1104I CTCTTC 5 cut(s) 482, 671, 1532, 1826, 2021
EarI CTCTTC 5 cut(s) 482, 671, 1532, 1826, 2021
EciI GGCGGA 7 cut(s) 160, 208, 258, 264, 285, 464, 627
Ecl136II GAGCTC 1 cut(s) 1525
Eco130I CCWWGG 3 cut(s) 363, 668, 1956
Eco24I GRGCYC 3 cut(s) 118, 662, 1527
Eco47I GGWCC 4 cut(s) 105, 360, 1873, 1994
Eco47III AGCGCT 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 1525
Eco57I CTGAAG 2 cut(s) 1415, 1469
Eco91I GGTNACC 1 cut(s) 863
EcoICRI GAGCTC 1 cut(s) 1525
EcoO109I RGGNCCY 2 cut(s) 114, 1994
EcoO65I GGTNACC 1 cut(s) 863
EcoRII CCWGG 3 cut(s) 237, 1676, 1888
EcoT14I CCWWGG 3 cut(s) 363, 668, 1956
EcoT22I ATGCAT 2 cut(s) 1406, 1758
EcoT38I GRGCYC 3 cut(s) 118, 662, 1527
ErhI CCWWGG 3 cut(s) 363, 668, 1956
Esp3I CGTCTC 1 cut(s) 184
FalI AAGNNNNNCTT 4 cut(s) 1212, 1244, 1511, 1543
FaqI GGGAC 3 cut(s) 373, 448, 805
FauI CCCGC 3 cut(s) 252, 313, 458
FbaI TGATCA 1 cut(s) 42
FblI GTMKAC 1 cut(s) 1902
FokI GGATG 7 cut(s) 899, 906, 1153, 1283, 1340, 1892, 1943
FriOI GRGCYC 3 cut(s) 118, 662, 1527
FspBI CTAG 2 cut(s) 1205, 1319
GlaI GCGC 3 cut(s) 155, 309, 587
GsaI CCCAGC 2 cut(s) 121, 1255
HaeII RGCGCY 1 cut(s) 589
HaeIII GGCC 9 cut(s) 116, 242, 368, 426, 551, 673, 1453, 1707, 1818
HapII CCGG 7 cut(s) 108, 459, 605, 612, 782, 1147, 1370
HhaI GCGC 3 cut(s) 156, 310, 588
Hin6I GCGC 3 cut(s) 154, 308, 586
HinP1I GCGC 3 cut(s) 154, 308, 586
HincII GTYRAC 2 cut(s) 1303, 1854
HindII GTYRAC 2 cut(s) 1303, 1854
HinfI GANTC 6 cut(s) 608, 976, 1102, 1355, 1413, 1538
HpaI GTTAAC 1 cut(s) 1303
HpaII CCGG 7 cut(s) 108, 459, 605, 612, 782, 1147, 1370
HphI GGTGA 3 cut(s) 70, 127, 857
Hpy166II GTNNAC 5 cut(s) 349, 1303, 1854, 1895, 1903
Hpy188I TCNGA 6 cut(s) 322, 744, 981, 1395, 1488, 1990
Hpy8I GTNNAC 5 cut(s) 349, 1303, 1854, 1895, 1903
Hpy99I CGWCG 2 cut(s) 322, 374
HpyAV CCTTC 8 cut(s) 91, 142, 177, 217, 267, 297, 1390, 1559
HpyCH4III ACNGT 7 cut(s) 178, 512, 531, 798, 1497, 1736, 1877
HpyCH4IV ACGT 2 cut(s) 324, 1897
HpyF3I CTNAG 3 cut(s) 837, 1883, 1989
HpySE526I ACGT 2 cut(s) 324, 1897
HspAI GCGC 3 cut(s) 154, 308, 586
Kpn2I TCCGGA 1 cut(s) 604
Ksp22I TGATCA 1 cut(s) 42
KspAI GTTAAC 1 cut(s) 1303
LguI GCTCTTC 3 cut(s) 671, 1532, 2021
LmnI GCTCC 8 cut(s) 129, 420, 462, 961, 1149, 1210, 1433, 1522
Lsp1109I GCAGC 6 cut(s) 133, 912, 1034, 1184, 1298, 1823
LweI GCATC 6 cut(s) 1076, 1131, 1215, 1318, 1492, 1903
MabI ACCWGGT 1 cut(s) 1888
MaeI CTAG 2 cut(s) 1205, 1319
MaeII ACGT 2 cut(s) 324, 1897
MaeIII GTNAC 5 cut(s) 76, 506, 863, 1106, 1441
MbiI CCGCTC 3 cut(s) 423, 465, 595
MfeI CAATTG 1 cut(s) 951
MflI RGATCY 1 cut(s) 33
MhlI GDGCHC 3 cut(s) 118, 662, 1527
MlsI TGGCCA 2 cut(s) 551, 1453
MluCI AATT 9 cut(s) 415, 625, 951, 986, 1096, 1560, 1666, 1672, 1972
MluNI TGGCCA 2 cut(s) 551, 1453
MlyI GAGTC 3 cut(s) 1096, 1349, 1407
MmeI TCCRAC 2 cut(s) 300, 871
Mox20I TGGCCA 2 cut(s) 551, 1453
Mph1103I ATGCAT 2 cut(s) 1406, 1758
MroI TCCGGA 1 cut(s) 604
MscI TGGCCA 2 cut(s) 551, 1453
MslI CAYNNNNRTG 1 cut(s) 944
Msp20I TGGCCA 2 cut(s) 551, 1453
MspA1I CMGCKG 3 cut(s) 334, 1311, 1814
MspI CCGG 7 cut(s) 108, 459, 605, 612, 782, 1147, 1370
MspR9I CCNGG 7 cut(s) 239, 460, 783, 1148, 1370, 1678, 1890
MunI CAATTG 1 cut(s) 951
MvaI CCWGG 3 cut(s) 239, 1678, 1890
MvnI CGCG 3 cut(s) 154, 308, 684
NciI CCSGG 4 cut(s) 460, 783, 1148, 1370
NcoI CCATGG 3 cut(s) 363, 668, 1956
NlaIV GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 636, 1695, 1708, 1995
NmuCI GTSAC 4 cut(s) 76, 863, 1106, 1441
NsiI ATGCAT 2 cut(s) 1406, 1758
NspI RCATGY 3 cut(s) 1404, 1465, 1811
PaeI GCATGC 2 cut(s) 1404, 1465
PagI TCATGA 1 cut(s) 66
PciSI GCTCTTC 3 cut(s) 671, 1532, 2021
PcsI WCGNNNNNNNCGW 4 cut(s) 18, 384, 390, 516
PfeI GAWTC 3 cut(s) 608, 976, 1538
PflMI CCANNNNNTGG 2 cut(s) 669, 1962
PfoI TCCNGGA 1 cut(s) 781
PinAI ACCGGT 1 cut(s) 107
PleI GAGTC 3 cut(s) 1096, 1349, 1407
PpsI GAGTC 3 cut(s) 1096, 1349, 1407
PpuMI RGGWCCY 1 cut(s) 1994
PshBI ATTAAT 1 cut(s) 1095
PsiI TTATAA 1 cut(s) 1188
Psp124BI GAGCTC 1 cut(s) 1527
Psp5II RGGWCCY 1 cut(s) 1994
Psp6I CCWGG 3 cut(s) 237, 1676, 1888
PspEI GGTNACC 1 cut(s) 863
PspFI CCCAGC 2 cut(s) 117, 1251
PspGI CCWGG 3 cut(s) 237, 1676, 1888
PspN4I GGNNCC 9 cut(s) 35, 115, 116, 243, 361, 636, 1695, 1708, 1995
PspOMI GGGCCC 1 cut(s) 114
PspPI GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1706, 1873, 1994
PspPPI RGGWCCY 1 cut(s) 1994
PsuI RGATCY 1 cut(s) 33
PvuII CAGCTG 2 cut(s) 1311, 1814
RsaI GTAC 4 cut(s) 524, 708, 821, 1049
RsaNI GTAC 4 cut(s) 523, 707, 820, 1048
RseI CAYNNNNRTG 1 cut(s) 944
SacI GAGCTC 1 cut(s) 1527
SapI GCTCTTC 3 cut(s) 671, 1532, 2021
Sau96I GGNCC 8 cut(s) 105, 114, 115, 241, 360, 1706, 1873, 1994
ScaI AGTACT 1 cut(s) 821
SchI GAGTC 3 cut(s) 1096, 1349, 1407
ScrFI CCNGG 7 cut(s) 239, 460, 783, 1148, 1370, 1678, 1890
SduI GDGCHC 3 cut(s) 118, 662, 1527
SexAI ACCWGGT 1 cut(s) 1888
SfaNI GCATC 6 cut(s) 1076, 1131, 1215, 1318, 1492, 1903
SfcI CTRYAG 1 cut(s) 2008
SinI GGWCC 4 cut(s) 105, 360, 1873, 1994
SmiI ATTTAAAT 1 cut(s) 1671
SmiMI CAYNNNNRTG 1 cut(s) 944
SmlI CTYRAG 4 cut(s) 995, 1127, 1357, 1865
SmoI CTYRAG 4 cut(s) 995, 1127, 1357, 1865
SphI GCATGC 2 cut(s) 1404, 1465
Sse9I AATT 9 cut(s) 415, 625, 951, 986, 1096, 1560, 1666, 1672, 1972
SspI AATATT 1 cut(s) 1547
SspMI CTAG 2 cut(s) 1205, 1319
SstI GAGCTC 1 cut(s) 1527
StyD4I CCNGG 7 cut(s) 237, 458, 781, 1146, 1368, 1676, 1888
StyI CCWWGG 3 cut(s) 363, 668, 1956
SwaI ATTTAAAT 1 cut(s) 1671
TaaI ACNGT 7 cut(s) 178, 512, 531, 798, 1497, 1736, 1877
TaiI ACGT 2 cut(s) 327, 1900
TaqI TCGA 3 cut(s) 378, 393, 874
TasI AATT 9 cut(s) 415, 625, 951, 986, 1096, 1560, 1666, 1672, 1972
TatI WGTACW 2 cut(s) 522, 819
TfiI GAWTC 3 cut(s) 608, 976, 1538
TscAI CASTG 2 cut(s) 1220, 1653
TseFI GTSAC 4 cut(s) 76, 863, 1106, 1441
TseI GCWGC 6 cut(s) 121, 925, 1022, 1172, 1311, 1811
Tsp45I GTSAC 4 cut(s) 76, 863, 1106, 1441
TspDTI ATGAA 5 cut(s) 52, 1209, 1569, 1700, 2008
TspGWI ACGGA 4 cut(s) 64, 946, 1565, 1898
TspRI CASTG 2 cut(s) 1220, 1653
Van91I CCANNNNNTGG 2 cut(s) 669, 1962
VpaK11BI GGWCC 4 cut(s) 105, 360, 1873, 1994
VspI ATTAAT 1 cut(s) 1095
XapI RAATTY 1 cut(s) 1672
XceI RCATGY 3 cut(s) 1404, 1465, 1811
XmiI GTMKAC 1 cut(s) 1902
XspI CTAG 2 cut(s) 1205, 1319
ZrmI AGTACT 1 cut(s) 821
Zsp2I ATGCAT 2 cut(s) 1406, 1758
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.