RchiOBHm_Chr1g0374801

mitochondrial chaperone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018082
Physical Location & Seq
Forward (+)
62498475 .. 62500197
1723 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGTCGACCTTGTTCTCAACTGCTGCCTCCTTTGCTGCAACACTGATGTTGGGCCGTTCCATGGTGGATCAAGTCTTCAACAACTCATACCTCCAGGCTCTCTGGGCTTGGTTCCGGTCTGATCTGACTTTTGTTGTCCCGGAGTACACCGAAGTAGGAGAACGTAATCAGATATACGACTCCGTGGAGCCCTACCTCCGAGCAAAAATAGGCACCAATACGAAACGTGTCAGAGTCAGCAAAACCACTAGGCAAAAGACCACCAGCTTTACCCTTGACGACTATCAAGAAGTCACAGACACATTTGAAAACTTCAGTATTAAGTGGCGTTATGATCATCACTCTGAAGAAAAACGTTACTATGAACTAAATTTCCACAAGAAGCACAGAGCCAAAATGAGCTCCTACTTGGACTATGTCGTTGCTAAAGCTAAAGAGATCAAAAATGCAGAAAAGGTTCTCAGGATTTATTCTCGTTGCACTTCAGGTTCGTACAAATACTGGGAATCAACAATATTAGAGCATCCTTGTACTTTTGAGACACTGGCCATGGATCCAACGGAGAAGAAAATGATCATGGACGATTTGGATAGGTTTGTGAGGAGGAAAGAGTTCTATAAGAAGGTTGGGAAGGCGTGGAAAAGAGGCTACTTGTTATATGGTCCGCCTGGTACTGGCAAATCCAGCTTGATTGCAGCCATGGCAAATTACCTCAACTACAATATATATGATTTGGAGCTGACAAGCATCTCCAACAATTCTGATTTGAGGCATTCTTTGCTGTCCACCACCAATCGCTCTATTTTGATAATTGAGGACATTGATTGCTCCTTGGATATACAGAACCGGGAAAAAGAGGAGGAATCAGATGAGCAAGTGCTTGTCCTTACTAAGGACAAGAACGAACTAAAGCAACAGACTGTGACACTGTCGGGGCTACTGAACTTCATTGATGGCCTGTGGTCAAGCTGTGGTGATGAGAGAATCATTGTGTTCACCACCAACTACAAGGATAAACTAGACCCTGCATTGTTGCGGCCAGGACGAATGGATGTGCATATACACATGTCATATTGCACTGTGAATGGATTCAAGATCCTGGCTTCTAGGTACCTTGACATTCATGATCATCCTCTCTTTGCAGAAATTGAAGGGTTGATGCAGACCACAGAGGTCACCCCGGCACAGGTAGCTGGGCAGCTACAGGTGAGTTATGATGCTGATGTTGCTCTTGCAGCGCTGGTGAAATTCATCAAGGAAAAGAAGGAGGAGGATGAGAAGAAGGAGAAGGAAAAAAAGAAAGAAGCACAAGCAAAGAAACTAAGGAGAAAAAGGTTGCTGGCTCTTTTTCAGAAGATCAAGGAAATTACATAA

Protein Analysis

457

Amino Acids

53.13

Weight (kDa)

8.61

Isoelectric Point (pI)

43.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 24 - 111 3.8e-18 Domain associated at C-terminal with AAA
AAA PF00004 217 - 357 2.6e-19 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 359 - 430 1.1e-22 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018082)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g27860
rosa_chinensis RchiOBHm_Chr1g0374801
rosa_laevigata RLG00000026714 RLG00000026717
rosa_multiflora Rmu_co8506051.1_g000001
rosa_roxburghii Rroxscaffold_4G00283270
rosa_rugosa Rorug01G0384900
rosa_samantha Rh1AG396400 Rh1BG360500 Rh1CG373900
rosa_wichuraiana Rw1G035140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1110
AccB1I GGYRCC 2 cut(s) 212, 1110
AccI GTMKAC 1 cut(s) 5
AciI CCGC 2 cut(s) 665, 1036
AclI AACGTT 1 cut(s) 355
AclWI GGATC 4 cut(s) 75, 548, 561, 1090
AcoI YGGCCR 2 cut(s) 546, 1037
AcsI RAATTY 2 cut(s) 370, 1247
AcuI CTGAAG 3 cut(s) 298, 366, 468
AfaI GTAC 5 cut(s) 146, 494, 532, 673, 1112
AfeI AGCGCT 1 cut(s) 1239
AfiI CCNNNNNNNGG 4 cut(s) 61, 674, 892, 1186
AflIII ACRYGT 2 cut(s) 226, 1065
AgsI TTSAA 4 cut(s) 79, 308, 1093, 1151
AjnI CCWGG 4 cut(s) 93, 667, 1039, 1098
AloI GAACNNNNNNTCC 2 cut(s) 357, 389
AluBI AGCT 8 cut(s) 267, 402, 431, 687, 739, 969, 1193, 1201
AluI AGCT 8 cut(s) 267, 402, 431, 687, 739, 969, 1193, 1201
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 1 cut(s) 533
AlwI GGATC 4 cut(s) 75, 548, 561, 1090
Aor51HI AGCGCT 1 cut(s) 1239
AoxI GGCC 4 cut(s) 52, 546, 955, 1037
ApeKI GCWGC 5 cut(s) 23, 35, 695, 1198, 1235
ApoI RAATTY 2 cut(s) 370, 1247
Asp700I GAANNNNTTC 3 cut(s) 456, 611, 1088
Asp718I GGTACC 1 cut(s) 1110
AspLEI GCGC 1 cut(s) 1240
AspS9I GGNCC 2 cut(s) 52, 662
AsuC2I CCSGG 3 cut(s) 140, 848, 1181
AsuHPI GGTGA 5 cut(s) 986, 988, 1168, 1219, 1255
AvaII GGWCC 1 cut(s) 662
BalI TGGCCA 1 cut(s) 548
BamHI GGATCC 1 cut(s) 553
BanI GGYRCC 2 cut(s) 212, 1110
BanII GRGCYC 2 cut(s) 192, 404
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 5 cut(s) 10, 22, 707, 1210, 1247
BccI CCATC 1 cut(s) 947
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 4 cut(s) 95, 669, 1041, 1100
BclI TGATCA 3 cut(s) 334, 573, 1126
BcnI CCSGG 3 cut(s) 140, 848, 1181
BcoDI GTCTC 1 cut(s) 533
BfaI CTAG 3 cut(s) 249, 1019, 1107
BfmI CTRYAG 1 cut(s) 1202
BfoI RGCGCY 1 cut(s) 1241
BisI GCNGC 6 cut(s) 24, 36, 696, 1037, 1199, 1236
BlsI GCNGC 6 cut(s) 25, 37, 697, 1038, 1200, 1237
Bme1390I CCNGG 7 cut(s) 95, 140, 669, 848, 1041, 1100, 1181
Bme18I GGWCC 1 cut(s) 662
BmgT120I GGNCC 2 cut(s) 52, 662
BmiI GGNNCC 5 cut(s) 113, 189, 214, 555, 1112
BmrFI CCNGG 7 cut(s) 95, 140, 669, 848, 1041, 1100, 1181
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 4 cut(s) 532, 756, 1149, 1207
BmuI ACTGGG 1 cut(s) 511
BpiI GAAGAC 1 cut(s) 67
BpmI CTGGAG 1 cut(s) 77
BpuMI CCSGG 3 cut(s) 140, 848, 1181
BsaJI CCNNGG 6 cut(s) 60, 183, 549, 699, 831, 1179
BsaWI WCCGGW 1 cut(s) 114
Bsc4I CCNNNNNNNGG 4 cut(s) 61, 674, 892, 1186
Bse1I ACTGG 3 cut(s) 506, 549, 679
BseBI CCWGG 4 cut(s) 95, 669, 1041, 1100
BseDI CCNNGG 6 cut(s) 60, 183, 549, 699, 831, 1179
BseGI GGATG 4 cut(s) 523, 1057, 1129, 1279
BseLI CCNNNNNNNGG 4 cut(s) 61, 674, 892, 1186
BseMII CTCAG 1 cut(s) 475
BseNI ACTGG 3 cut(s) 506, 549, 679
BseRI GAGGAG 3 cut(s) 616, 872, 1283
BseXI GCAGC 5 cut(s) 10, 22, 707, 1210, 1247
BseYI CCCAGC 1 cut(s) 1193
BshFI GGCC 4 cut(s) 54, 548, 957, 1039
BshNI GGYRCC 2 cut(s) 212, 1110
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 4 cut(s) 115, 140, 847, 1181
BslFI GGGAC 1 cut(s) 122
BslI CCNNNNNNNGG 4 cut(s) 61, 674, 892, 1186
BsmAI GTCTC 1 cut(s) 533
BsmFI GGGAC 1 cut(s) 122
BsmI GAATGC 1 cut(s) 772
BsnI GGCC 4 cut(s) 54, 548, 957, 1039
Bsp1286I GDGCHC 2 cut(s) 192, 404
Bsp143I GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
Bsp19I CCATGG 3 cut(s) 60, 549, 699
BspACI CCGC 2 cut(s) 665, 1036
BspANI GGCC 4 cut(s) 54, 548, 957, 1039
BspCNI CTCAG 1 cut(s) 474
BspHI TCATGA 1 cut(s) 1123
BspLI GGNNCC 5 cut(s) 113, 189, 214, 555, 1112
BspPI GGATC 4 cut(s) 75, 548, 561, 1090
BspT107I GGYRCC 2 cut(s) 212, 1110
BsrI ACTGG 3 cut(s) 506, 549, 679
BssECI CCNNGG 6 cut(s) 60, 183, 549, 699, 831, 1179
BssMI GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
BssT1I CCWWGG 4 cut(s) 60, 549, 699, 831
Bst2UI CCWGG 4 cut(s) 95, 669, 1041, 1100
Bst4CI ACNGT 3 cut(s) 922, 930, 1081
BstAPI GCANNNNNTGC 1 cut(s) 778
BstC8I GCNNGC 1 cut(s) 1341
BstDEI CTNAG 3 cut(s) 461, 891, 1322
BstDSI CCRYGG 4 cut(s) 60, 183, 549, 699
BstEII GGTNACC 1 cut(s) 1174
BstENI CCTNNNNNAGG 1 cut(s) 890
BstF5I GGATG 4 cut(s) 523, 1057, 1129, 1279
BstH2I RGCGCY 1 cut(s) 1241
BstHHI GCGC 1 cut(s) 1240
BstKTI GATC 9 cut(s) 70, 124, 337, 441, 556, 576, 1098, 1129, 1359
BstMAI GTCTC 1 cut(s) 533
BstMBI GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
BstMWI GCNNNNNNNGC 8 cut(s) 32, 104, 684, 701, 778, 1190, 1226, 1235
BstNI CCWGG 4 cut(s) 95, 669, 1041, 1100
BstNSI RCATGY 1 cut(s) 1069
BstPI GGTNACC 1 cut(s) 1174
BstSCI CCNGG 7 cut(s) 93, 138, 667, 846, 1039, 1098, 1179
BstSFI CTRYAG 1 cut(s) 1202
BstV1I GCAGC 5 cut(s) 10, 22, 707, 1210, 1247
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 2 cut(s) 553, 1095
BstYI RGATCY 2 cut(s) 553, 1095
BsuRI GGCC 4 cut(s) 54, 548, 957, 1039
BtgI CCRYGG 4 cut(s) 60, 183, 549, 699
BtsCI GGATG 4 cut(s) 523, 1057, 1129, 1279
BtsIMutI CAGTG 4 cut(s) 41, 542, 926, 1077
Cac8I GCNNGC 1 cut(s) 1341
CciI TCATGA 1 cut(s) 1123
CfoI GCGC 1 cut(s) 1240
Cfr13I GGNCC 2 cut(s) 52, 662
Csp6I GTAC 5 cut(s) 145, 493, 531, 672, 1111
CviAII CATG 6 cut(s) 61, 550, 577, 700, 1066, 1124
CviQI GTAC 5 cut(s) 145, 493, 531, 672, 1111
DdeI CTNAG 3 cut(s) 461, 891, 1322
DpnI GATC 9 cut(s) 69, 123, 336, 440, 555, 575, 1097, 1128, 1358
DpnII GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
EaeI YGGCCR 2 cut(s) 546, 1037
EciI GGCGGA 1 cut(s) 654
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 4 cut(s) 60, 549, 699, 831
Eco24I GRGCYC 2 cut(s) 192, 404
Eco47I GGWCC 1 cut(s) 662
Eco47III AGCGCT 1 cut(s) 1239
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 3 cut(s) 298, 366, 468
Eco91I GGTNACC 1 cut(s) 1174
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 890
EcoO65I GGTNACC 1 cut(s) 1174
EcoRII CCWGG 4 cut(s) 93, 667, 1039, 1098
EcoT14I CCWWGG 4 cut(s) 60, 549, 699, 831
EcoT38I GRGCYC 2 cut(s) 192, 404
ErhI CCWWGG 4 cut(s) 60, 549, 699, 831
FaeI CATG 6 cut(s) 64, 553, 580, 703, 1069, 1127
FaqI GGGAC 1 cut(s) 122
FatI CATG 6 cut(s) 60, 549, 576, 699, 1065, 1123
FbaI TGATCA 3 cut(s) 334, 573, 1126
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 6 cut(s) 24, 36, 696, 1037, 1199, 1236
FokI GGATG 4 cut(s) 510, 1064, 1116, 1286
FriOI GRGCYC 2 cut(s) 192, 404
Fsp4HI GCNGC 6 cut(s) 24, 36, 696, 1037, 1199, 1236
FspBI CTAG 3 cut(s) 249, 1019, 1107
GlaI GCGC 1 cut(s) 1239
GluI GCNGC 6 cut(s) 24, 36, 696, 1037, 1199, 1236
GsaI CCCAGC 1 cut(s) 1197
GsuI CTGGAG 1 cut(s) 77
HaeII RGCGCY 1 cut(s) 1241
HaeIII GGCC 4 cut(s) 54, 548, 957, 1039
HapII CCGG 4 cut(s) 115, 140, 847, 1181
HhaI GCGC 1 cut(s) 1240
Hin1II CATG 6 cut(s) 64, 553, 580, 703, 1069, 1127
Hin6I GCGC 1 cut(s) 1238
HinP1I GCGC 1 cut(s) 1238
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 6 cut(s) 179, 234, 506, 863, 984, 1089
HpaII CCGG 4 cut(s) 115, 140, 847, 1181
HphI GGTGA 5 cut(s) 986, 988, 1168, 1219, 1255
Hpy166II GTNNAC 4 cut(s) 6, 147, 786, 996
Hpy188I TCNGA 9 cut(s) 121, 126, 171, 200, 233, 346, 763, 868, 1353
Hpy188III TCNNGA 4 cut(s) 287, 463, 1093, 1124
Hpy8I GTNNAC 4 cut(s) 6, 147, 786, 996
HpyAV CCTTC 6 cut(s) 616, 625, 1145, 1258, 1276, 1282
HpyCH4III ACNGT 3 cut(s) 922, 930, 1081
HpyCH4IV ACGT 3 cut(s) 163, 226, 355
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 104, 684, 701, 778, 1190, 1226, 1235
HpyF3I CTNAG 3 cut(s) 461, 891, 1322
HpySE526I ACGT 3 cut(s) 163, 226, 355
Hsp92II CATG 6 cut(s) 64, 553, 580, 703, 1069, 1127
HspAI GCGC 1 cut(s) 1238
KpnI GGTACC 1 cut(s) 1114
Ksp22I TGATCA 3 cut(s) 334, 573, 1126
Kzo9I GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
LmnI GCTCC 4 cut(s) 187, 407, 736, 833
Lsp1109I GCAGC 5 cut(s) 10, 22, 707, 1210, 1247
LweI GCATC 4 cut(s) 532, 756, 1149, 1207
MaeI CTAG 3 cut(s) 249, 1019, 1107
MaeII ACGT 3 cut(s) 163, 226, 355
MaeIII GTNAC 4 cut(s) 292, 356, 922, 1174
MalI GATC 9 cut(s) 69, 123, 336, 440, 555, 575, 1097, 1128, 1358
MboI GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
MboII GAAGA 5 cut(s) 67, 359, 577, 1291, 1366
MflI RGATCY 2 cut(s) 553, 1095
MhlI GDGCHC 2 cut(s) 192, 404
MlsI TGGCCA 1 cut(s) 548
MluCI AATT 7 cut(s) 370, 706, 757, 810, 1146, 1247, 1365
MluNI TGGCCA 1 cut(s) 548
MlyI GAGTC 2 cut(s) 173, 243
MmeI TCCRAC 2 cut(s) 581, 777
Mox20I TGGCCA 1 cut(s) 548
MroXI GAANNNNTTC 3 cut(s) 456, 611, 1088
MscI TGGCCA 1 cut(s) 548
MseI TTAA 1 cut(s) 321
Msp20I TGGCCA 1 cut(s) 548
MspI CCGG 4 cut(s) 115, 140, 847, 1181
MspR9I CCNGG 7 cut(s) 95, 140, 669, 848, 1041, 1100, 1181
Mva1269I GAATGC 1 cut(s) 772
MvaI CCWGG 4 cut(s) 95, 669, 1041, 1100
MwoI GCNNNNNNNGC 8 cut(s) 32, 104, 684, 701, 778, 1190, 1226, 1235
NciI CCSGG 3 cut(s) 140, 848, 1181
NcoI CCATGG 3 cut(s) 60, 549, 699
NdeII GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
NlaIII CATG 6 cut(s) 64, 553, 580, 703, 1069, 1127
NlaIV GGNNCC 5 cut(s) 113, 189, 214, 555, 1112
NmuCI GTSAC 3 cut(s) 292, 922, 1174
NspI RCATGY 1 cut(s) 1069
PagI TCATGA 1 cut(s) 1123
PciI ACATGT 1 cut(s) 1065
PctI GAATGC 1 cut(s) 772
PdmI GAANNNNTTC 3 cut(s) 456, 611, 1088
PfeI GAWTC 4 cut(s) 506, 863, 984, 1089
PflFI GACNNNGTC 2 cut(s) 416, 928
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 6 cut(s) 25, 37, 697, 1038, 1200, 1237
PleI GAGTC 2 cut(s) 173, 242
PpsI GAGTC 2 cut(s) 173, 242
PscI ACATGT 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 404
Psp1406I AACGTT 1 cut(s) 355
Psp6I CCWGG 4 cut(s) 93, 667, 1039, 1098
PspEI GGTNACC 1 cut(s) 1174
PspFI CCCAGC 1 cut(s) 1193
PspGI CCWGG 4 cut(s) 93, 667, 1039, 1098
PspN4I GGNNCC 5 cut(s) 113, 189, 214, 555, 1112
PspPI GGNCC 2 cut(s) 52, 662
PsuI RGATCY 2 cut(s) 553, 1095
PsyI GACNNNGTC 2 cut(s) 416, 928
RsaI GTAC 5 cut(s) 146, 494, 532, 673, 1112
RsaNI GTAC 5 cut(s) 145, 493, 531, 672, 1111
SacI GAGCTC 1 cut(s) 404
SalI GTCGAC 1 cut(s) 4
SaqAI TTAA 1 cut(s) 321
SatI GCNGC 6 cut(s) 24, 36, 696, 1037, 1199, 1236
Sau3AI GATC 9 cut(s) 67, 121, 334, 438, 553, 573, 1095, 1126, 1356
Sau96I GGNCC 2 cut(s) 52, 662
SchI GAGTC 2 cut(s) 173, 243
ScrFI CCNGG 7 cut(s) 95, 140, 669, 848, 1041, 1100, 1181
SduI GDGCHC 2 cut(s) 192, 404
SfaNI GCATC 4 cut(s) 532, 756, 1149, 1207
SfcI CTRYAG 1 cut(s) 1202
SinI GGWCC 1 cut(s) 662
Sse9I AATT 7 cut(s) 370, 706, 757, 810, 1146, 1247, 1365
SsiI CCGC 2 cut(s) 665, 1036
SspI AATATT 1 cut(s) 516
SspMI CTAG 3 cut(s) 249, 1019, 1107
SstI GAGCTC 1 cut(s) 404
StyD4I CCNGG 7 cut(s) 93, 138, 667, 846, 1039, 1098, 1179
StyI CCWWGG 4 cut(s) 60, 549, 699, 831
TaaI ACNGT 3 cut(s) 922, 930, 1081
TaiI ACGT 3 cut(s) 166, 229, 358
TaqI TCGA 1 cut(s) 5
TasI AATT 7 cut(s) 370, 706, 757, 810, 1146, 1247, 1365
TatI WGTACW 2 cut(s) 144, 530
TauI GCSGC 1 cut(s) 1039
TfiI GAWTC 4 cut(s) 506, 863, 984, 1089
Tru1I TTAA 1 cut(s) 321
Tru9I TTAA 1 cut(s) 321
TscAI CASTG 4 cut(s) 48, 549, 933, 1084
TseFI GTSAC 3 cut(s) 292, 922, 1174
TseI GCWGC 5 cut(s) 23, 35, 695, 1198, 1235
Tsp45I GTSAC 3 cut(s) 292, 922, 1174
TspDTI ATGAA 4 cut(s) 378, 937, 1112, 1240
TspGWI ACGGA 2 cut(s) 172, 575
TspRI CASTG 4 cut(s) 48, 549, 933, 1084
Tth111I GACNNNGTC 2 cut(s) 416, 928
VpaK11BI GGWCC 1 cut(s) 662
XagI CCTNNNNNAGG 1 cut(s) 890
XapI RAATTY 2 cut(s) 370, 1247
XceI RCATGY 1 cut(s) 1069
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 3 cut(s) 456, 611, 1088
XspI CTAG 3 cut(s) 249, 1019, 1107
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.