RchiOBHm_Chr1g0374941

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0017718
Physical Location & Seq
Reverse (-)
62572795 .. 62573813
1019 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 873 bp
ATGAGTGACATGACTCCCTTGGGTCTAGGCGTTTCGCCAAAGCACGAGTTGGGAATGATGAAGGAAGGCTACGTTTGGATCATGACCAATGGGCTAACCAGTCTGTTGAGTAACGGTTCAGTCATCAACTCAATGCAAGGGGCATTGGGTGTGAGGGTTTATGTGCCAAAAACAGACAAGCTTATCGACTTCAGGGCCCGATGGAAAAGGAAATTTCAACAAGAAAATCCGACCATATGTAGCATGGCAGTTGAGAATGTTGCCACTAGATCAACCTTTGGCTTCCGAAAGACAAATGATTCCATCAACTCATCGGCAGATCTCGAAACTCTCAGGGTCTCTAGAAAAGGTCTAGAAATTTCCAAGTTCTTATCAAGTACTAGATTTAGAGGCGGCATTGCAGGAGATTTCAGTTTTGTCAACGGGCAGTTACAATCACCGATGTTCGAAATTGTTAATGTGAATGGTAATGGAGTAAGAGGCATTGGGTTTTGGACACCACAACGTGGACTTGTGAAAAGTTCGAACGTACACGGTTGGGAAGTTCCCACAAACGGCAAAAAGTTGAGAGTAGGAGTTCCGGTGAAGATTGGTTCTCCGGAGCTTGTGAAGGTGGTGCGTAATCCTGTCACTAATGGAACAGAAGTCACTGAGTTCTGCATTGACATCATCAAGGCTGTAATGGAAGGATTACCCTACGCAGTTACTTATGACTTCATCCCCTTTGCAAAGCCCGATGGTACTACTGCCGGCAGCTACAATGACTTGGTCCATCAAGCTTTTCTTGGGCTAAAATCATTTCAACTCATTTACTTTCTAACATCAATCCTACATAACAATACTGAAATGATATCAAATATAGCGCTACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

290

Amino Acids

31.92

Weight (kDa)

9.49

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 16 - 157 9.8e-14 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 506
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 1 cut(s) 393
AclWI GGATC 1 cut(s) 86
AcsI RAATTY 2 cut(s) 212, 357
AcuI CTGAAG 1 cut(s) 175
AdeI CACNNNGTG 1 cut(s) 506
AfaI GTAC 3 cut(s) 379, 531, 742
AfeI AGCGCT 1 cut(s) 864
AfiI CCNNNNNNNGG 2 cut(s) 506, 554
AgsI TTSAA 2 cut(s) 218, 803
AluBI AGCT 4 cut(s) 181, 604, 756, 779
AluI AGCT 4 cut(s) 181, 604, 756, 779
Alw26I GTCTC 1 cut(s) 343
AlwI GGATC 1 cut(s) 86
Aor13HI TCCGGA 1 cut(s) 598
Aor51HI AGCGCT 1 cut(s) 864
AoxI GGCC 1 cut(s) 195
ApaI GGGCCC 1 cut(s) 199
ApeKI GCWGC 1 cut(s) 753
ApoI RAATTY 2 cut(s) 212, 357
AspLEI GCGC 1 cut(s) 865
AspS9I GGNCC 3 cut(s) 195, 196, 769
AsuHPI GGTGA 2 cut(s) 429, 595
AsuII TTCGAA 2 cut(s) 447, 524
AvaII GGWCC 1 cut(s) 769
BaeGI GKGCMC 1 cut(s) 199
BanII GRGCYC 1 cut(s) 199
BarI GAAGNNNNNNTAC 2 cut(s) 53, 85
BauI CACGAG 1 cut(s) 44
BbvI GCAGC 1 cut(s) 765
BccI CCATC 4 cut(s) 195, 311, 731, 780
BceAI ACGGC 1 cut(s) 571
BcoDI GTCTC 1 cut(s) 343
BfaI CTAG 5 cut(s) 26, 267, 342, 353, 381
BfoI RGCGCY 1 cut(s) 866
BglII AGATCT 1 cut(s) 319
BisI GCNGC 2 cut(s) 394, 754
BlsI GCNGC 2 cut(s) 395, 755
BmcAI AGTACT 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 769
BmgT120I GGNCC 3 cut(s) 195, 196, 769
BmiI GGNNCC 1 cut(s) 197
Bpu14I TTCGAA 2 cut(s) 447, 524
BsaI GGTCTC 1 cut(s) 343
BsaJI CCNNGG 1 cut(s) 18
BsaWI WCCGGW 2 cut(s) 580, 598
BsaXI ACNNNNNCTCC 2 cut(s) 567, 597
Bsc4I CCNNNNNNNGG 2 cut(s) 506, 554
Bse118I RCCGGY 1 cut(s) 749
Bse1I ACTGG 1 cut(s) 99
Bse3DI GCAATG 1 cut(s) 396
BseAI TCCGGA 1 cut(s) 598
BseDI CCNNGG 1 cut(s) 18
BseGI GGATG 1 cut(s) 717
BseLI CCNNNNNNNGG 2 cut(s) 506, 554
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 346, 642
BseNI ACTGG 1 cut(s) 99
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 1 cut(s) 765
BshFI GGCC 1 cut(s) 197
BsiSI CCGG 3 cut(s) 581, 599, 750
BslI CCNNNNNNNGG 2 cut(s) 506, 554
BsmAI GTCTC 1 cut(s) 343
BsnI GGCC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 343
Bsp119I TTCGAA 2 cut(s) 447, 524
Bsp120I GGGCCC 1 cut(s) 195
Bsp1286I GDGCHC 1 cut(s) 199
Bsp13I TCCGGA 1 cut(s) 598
Bsp143I GATC 3 cut(s) 78, 269, 319
BspACI CCGC 1 cut(s) 393
BspANI GGCC 1 cut(s) 197
BspCNI CTCAG 2 cut(s) 345, 643
BspEI TCCGGA 1 cut(s) 598
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 1 cut(s) 86
BspT104I TTCGAA 2 cut(s) 447, 524
BspTNI GGTCTC 1 cut(s) 343
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 1 cut(s) 749
BsrI ACTGG 1 cut(s) 99
BssAI RCCGGY 1 cut(s) 749
BssECI CCNNGG 1 cut(s) 18
BssMI GATC 3 cut(s) 78, 269, 319
BssSI CACGAG 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 18
Bst2BI CACGAG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 116, 536
BstBI TTCGAA 2 cut(s) 447, 524
BstC8I GCNNGC 1 cut(s) 751
BstDEI CTNAG 2 cut(s) 332, 651
BstF5I GGATG 1 cut(s) 717
BstH2I RGCGCY 1 cut(s) 866
BstHHI GCGC 1 cut(s) 865
BstKTI GATC 3 cut(s) 81, 272, 322
BstMAI GTCTC 1 cut(s) 343
BstMBI GATC 3 cut(s) 78, 269, 319
BstSLI GKGCMC 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 765
BstX2I RGATCY 1 cut(s) 319
BstYI RGATCY 1 cut(s) 319
BsuRI GGCC 1 cut(s) 197
BtsCI GGATG 1 cut(s) 717
BtsIMutI CAGTG 1 cut(s) 648
Cac8I GCNNGC 1 cut(s) 751
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 865
Cfr10I RCCGGY 1 cut(s) 749
Cfr13I GGNCC 3 cut(s) 195, 196, 769
Csp6I GTAC 3 cut(s) 378, 530, 741
CviAII CATG 3 cut(s) 10, 82, 244
CviQI GTAC 3 cut(s) 378, 530, 741
DdeI CTNAG 2 cut(s) 332, 651
DpnI GATC 3 cut(s) 80, 271, 321
DpnII GATC 3 cut(s) 78, 269, 319
DraIII CACNNNGTG 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 199
Eco31I GGTCTC 1 cut(s) 343
Eco32I GATATC 1 cut(s) 852
Eco47I GGWCC 1 cut(s) 769
Eco47III AGCGCT 1 cut(s) 864
Eco57I CTGAAG 1 cut(s) 175
EcoO109I RGGNCCY 1 cut(s) 195
EcoRV GATATC 1 cut(s) 852
EcoT14I CCWWGG 1 cut(s) 18
EcoT38I GRGCYC 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 18
FaeI CATG 3 cut(s) 13, 85, 247
FaiI YATR 9 cut(s) 11, 83, 162, 236, 238, 245, 711, 834, 860
FalI AAGNNNNNCTT 2 cut(s) 768, 800
FatI CATG 3 cut(s) 9, 81, 243
FauNDI CATATG 1 cut(s) 236
Fnu4HI GCNGC 2 cut(s) 394, 754
FokI GGATG 1 cut(s) 704
FriOI GRGCYC 1 cut(s) 199
Fsp4HI GCNGC 2 cut(s) 394, 754
FspBI CTAG 5 cut(s) 26, 267, 342, 353, 381
GlaI GCGC 1 cut(s) 864
GluI GCNGC 2 cut(s) 394, 754
HaeII RGCGCY 1 cut(s) 866
HaeIII GGCC 1 cut(s) 197
HapII CCGG 3 cut(s) 581, 599, 750
HhaI GCGC 1 cut(s) 865
Hin1II CATG 3 cut(s) 13, 85, 247
Hin6I GCGC 1 cut(s) 863
HinP1I GCGC 1 cut(s) 863
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HindIII AAGCTT 2 cut(s) 179, 777
HinfI GANTC 2 cut(s) 13, 299
HpaII CCGG 3 cut(s) 581, 599, 750
HphI GGTGA 2 cut(s) 429, 595
Hpy166II GTNNAC 3 cut(s) 421, 509, 532
Hpy188I TCNGA 2 cut(s) 231, 287
Hpy188III TCNNGA 5 cut(s) 82, 323, 342, 353, 599
Hpy8I GTNNAC 3 cut(s) 421, 509, 532
HpyAV CCTTC 4 cut(s) 55, 59, 604, 680
HpyCH4III ACNGT 2 cut(s) 116, 536
HpyCH4IV ACGT 3 cut(s) 72, 505, 528
HpyCH4V TGCA 4 cut(s) 136, 401, 660, 728
HpyF3I CTNAG 2 cut(s) 332, 651
HpySE526I ACGT 3 cut(s) 72, 505, 528
Hsp92II CATG 3 cut(s) 13, 85, 247
HspAI GCGC 1 cut(s) 863
Kpn2I TCCGGA 1 cut(s) 598
KroI GCCGGC 1 cut(s) 749
KroNI GCCGGC 1 cut(s) 751
Kzo9I GATC 3 cut(s) 78, 269, 319
LmnI GCTCC 1 cut(s) 601
LpnPI CCDG 8 cut(s) 112, 178, 319, 387, 594, 612, 639, 763
Lsp1109I GCAGC 1 cut(s) 765
MaeI CTAG 5 cut(s) 26, 267, 342, 353, 381
MaeII ACGT 3 cut(s) 72, 505, 528
MaeIII GTNAC 6 cut(s) 5, 110, 429, 628, 646, 703
MalI GATC 3 cut(s) 80, 271, 321
MboI GATC 3 cut(s) 78, 269, 319
MboII GAAGA 1 cut(s) 598
MflI RGATCY 1 cut(s) 319
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 3 cut(s) 212, 357, 450
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 3 cut(s) 147, 383, 473
MroI TCCGGA 1 cut(s) 598
MroNI GCCGGC 1 cut(s) 749
MseI TTAA 1 cut(s) 456
MspI CCGG 3 cut(s) 581, 599, 750
NaeI GCCGGC 1 cut(s) 751
NdeI CATATG 1 cut(s) 236
NdeII GATC 3 cut(s) 78, 269, 319
NgoMIV GCCGGC 1 cut(s) 749
NlaIII CATG 3 cut(s) 13, 85, 247
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 3 cut(s) 5, 628, 646
NspV TTCGAA 2 cut(s) 447, 524
PagI TCATGA 1 cut(s) 81
PdiI GCCGGC 1 cut(s) 751
PfeI GAWTC 1 cut(s) 299
PflFI GACNNNGTC 1 cut(s) 767
PflMI CCANNNNNTGG 1 cut(s) 506
PkrI GCNGC 2 cut(s) 395, 755
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
PspN4I GGNNCC 1 cut(s) 197
PspOMI GGGCCC 1 cut(s) 195
PspPI GGNCC 3 cut(s) 195, 196, 769
PsuI RGATCY 1 cut(s) 319
PsyI GACNNNGTC 1 cut(s) 767
RsaI GTAC 3 cut(s) 379, 531, 742
RsaNI GTAC 3 cut(s) 378, 530, 741
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 2 cut(s) 394, 754
Sau3AI GATC 3 cut(s) 78, 269, 319
Sau96I GGNCC 3 cut(s) 195, 196, 769
ScaI AGTACT 1 cut(s) 379
SchI GAGTC 1 cut(s) 7
SduI GDGCHC 1 cut(s) 199
SfuI TTCGAA 2 cut(s) 447, 524
SinI GGWCC 1 cut(s) 769
Sse9I AATT 3 cut(s) 212, 357, 450
SsiI CCGC 1 cut(s) 393
SspMI CTAG 5 cut(s) 26, 267, 342, 353, 381
StyI CCWWGG 1 cut(s) 18
TaaI ACNGT 2 cut(s) 116, 536
TaiI ACGT 3 cut(s) 75, 508, 531
TaqI TCGA 4 cut(s) 186, 324, 447, 524
TasI AATT 3 cut(s) 212, 357, 450
TatI WGTACW 1 cut(s) 377
TauI GCSGC 1 cut(s) 396
TfiI GAWTC 1 cut(s) 299
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 3 cut(s) 5, 628, 646
TseI GCWGC 1 cut(s) 753
Tsp45I GTSAC 3 cut(s) 5, 628, 646
TspDTI ATGAA 2 cut(s) 74, 706
TspRI CASTG 1 cut(s) 655
Tth111I GACNNNGTC 1 cut(s) 767
Van91I CCANNNNNTGG 1 cut(s) 506
VpaK11BI GGWCC 1 cut(s) 769
XapI RAATTY 2 cut(s) 212, 357
XbaI TCTAGA 2 cut(s) 341, 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 241
XspI CTAG 5 cut(s) 26, 267, 342, 353, 381
ZrmI AGTACT 1 cut(s) 379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.