Rw1G035260

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0017718
Physical Location & Seq
Reverse (-)
63112791 .. 63115245
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G035260.1

Sequence Viewer

Length: 879 bp
ATGGCCATTGGAGAGAACAGAAGTACTATTCCGGTGAATGCGGGAGTGGTTCTTGACATGGATGATTCGCTAAGTACTGTTTGGTTGAGCTGCATCAACATGGCCCCGTCACTTTTGTGCATCTCATGGTTCGGCAAAGACGAGGCTGCAACAAGTCCTTCCCTCACCTTCCTCCGAAGTTCGTATTTATTCCAAGCTGCACAAAATGACTCCTCTCAAGTCAAAGCCATAAATGCTATTGTCCAAGCTTTTTGGTGGAGAAAAGCTGTGCCAATCTACATAGACAATGCCTTCGGCGAGGGGGTACTACCCTACTTGGTGGATGCCTTGCAAGAGGGTTGGGAAGTTCCCACAAACGGCAAAAAGTTGAGAGTAGGAGTTCCGGTGAAGATTGGTTCTCCGGAGTTTGTGAAGGTGGTGCGTAATCCTGTCACTAATGGAACAGAAGTCACTGAGTTCTGTATTGACATCATCAAGGCTGTAATGGAAGGATTACCCTACGCAGTTACTTATGACTTCATCCCCTTTGCAAAGCCCGATGAATTCGATGCTTTGGCCACAGCGACAACGGTTAGAGGGAACAGTACTGGCTCAAAGACTCAAAGTTACGGAGCTAGTCCATCATCGATACTAACTGTTCCACGGCTAAAACCAACTTATTCCGAGATAAACGAGCTTGTGAAGATTAGGGACTATGTGGGTTACCCAAAGGGTTCTTTTCTCTGCGACCTTTTGATACAAAGTGGTTTTGATGATTCTAAGATCGAGCCCTATATATCGCGGGAAGAATGTGACCAACTTCTGACAAAAGGGAGTGCAAATGGTGGTATTGCTACTGCTATTGACGAAACTCCCAACTTGAAGCTTGTTCTTGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

292

Amino Acids

31.75

Weight (kDa)

4.77

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 49 - 116 2.4e-11 Receptor family ligand binding region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 781
AccIII TCCGGA 1 cut(s) 400
AciI CCGC 2 cut(s) 41, 781
AcoI YGGCCR 2 cut(s) 3, 555
AcsI RAATTY 1 cut(s) 542
AfaI GTAC 4 cut(s) 25, 76, 306, 586
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 862
AleI CACNNNNGTG 1 cut(s) 115
AluBI AGCT 7 cut(s) 90, 197, 248, 266, 614, 676, 865
AluI AGCT 7 cut(s) 90, 197, 248, 266, 614, 676, 865
Aor13HI TCCGGA 1 cut(s) 400
AoxI GGCC 3 cut(s) 3, 102, 555
ApeKI GCWGC 3 cut(s) 90, 146, 197
ApoI RAATTY 1 cut(s) 542
ArsI GACNNNNNNTTYG 2 cut(s) 275, 307
AspS9I GGNCC 1 cut(s) 103
AsuHPI GGTGA 3 cut(s) 46, 157, 397
BalI TGGCCA 2 cut(s) 5, 557
BanII GRGCYC 1 cut(s) 771
BbvI GCAGC 3 cut(s) 77, 133, 184
BccI CCATC 1 cut(s) 628
BceAI ACGGC 2 cut(s) 373, 659
BfaI CTAG 1 cut(s) 615
BisI GCNGC 3 cut(s) 91, 147, 198
BlsI GCNGC 3 cut(s) 92, 148, 199
BmcAI AGTACT 3 cut(s) 25, 76, 586
BmgT120I GGNCC 1 cut(s) 103
BmiI GGNNCC 1 cut(s) 105
BmsI GCATC 4 cut(s) 102, 129, 313, 538
BpuEI CTTGAG 1 cut(s) 201
Bsa29I ATCGAT 1 cut(s) 626
BsaJI CCNNGG 1 cut(s) 641
BsaWI WCCGGW 3 cut(s) 31, 382, 400
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse1I ACTGG 1 cut(s) 592
BseAI TCCGGA 1 cut(s) 400
BseCI ATCGAT 1 cut(s) 626
BseDI CCNNGG 1 cut(s) 641
BseGI GGATG 3 cut(s) 67, 328, 519
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 1 cut(s) 592
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 3 cut(s) 77, 133, 184
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 781
BshFI GGCC 3 cut(s) 5, 104, 557
BshVI ATCGAT 1 cut(s) 626
BsiSI CCGG 3 cut(s) 32, 383, 401
BslFI GGGAC 1 cut(s) 704
BslI CCNNNNNNNGG 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 704
BsmI GAATGC 1 cut(s) 43
BsnI GGCC 3 cut(s) 5, 104, 557
Bsp1286I GDGCHC 1 cut(s) 771
Bsp13I TCCGGA 1 cut(s) 400
Bsp143I GATC 1 cut(s) 762
BspACI CCGC 2 cut(s) 41, 781
BspANI GGCC 3 cut(s) 5, 104, 557
BspCNI CTCAG 1 cut(s) 445
BspDI ATCGAT 1 cut(s) 626
BspEI TCCGGA 1 cut(s) 400
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 1 cut(s) 105
BsrI ACTGG 1 cut(s) 592
BssECI CCNNGG 1 cut(s) 641
BssMI GATC 1 cut(s) 762
Bst4CI ACNGT 4 cut(s) 79, 571, 584, 637
BstDEI CTNAG 3 cut(s) 71, 453, 759
BstDSI CCRYGG 1 cut(s) 641
BstEII GGTNACC 1 cut(s) 701
BstF5I GGATG 3 cut(s) 67, 328, 519
BstFNI CGCG 1 cut(s) 781
BstKTI GATC 1 cut(s) 765
BstMBI GATC 1 cut(s) 762
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstPI GGTNACC 1 cut(s) 701
BstUI CGCG 1 cut(s) 781
BstV1I GCAGC 3 cut(s) 77, 133, 184
Bsu15I ATCGAT 1 cut(s) 626
BsuRI GGCC 3 cut(s) 5, 104, 557
BsuTUI ATCGAT 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 641
BtsCI GGATG 3 cut(s) 67, 328, 519
BtsIMutI CAGTG 1 cut(s) 450
Cfr13I GGNCC 1 cut(s) 103
ClaI ATCGAT 1 cut(s) 626
Csp6I GTAC 4 cut(s) 24, 75, 305, 585
CviAII CATG 3 cut(s) 58, 100, 126
CviQI GTAC 4 cut(s) 24, 75, 305, 585
DdeI CTNAG 3 cut(s) 71, 453, 759
DpnI GATC 1 cut(s) 764
DpnII GATC 1 cut(s) 762
EaeI YGGCCR 2 cut(s) 3, 555
Eco24I GRGCYC 1 cut(s) 771
Eco91I GGTNACC 1 cut(s) 701
EcoO65I GGTNACC 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 771
FaeI CATG 3 cut(s) 61, 103, 129
FaiI YATR 9 cut(s) 59, 101, 127, 230, 281, 513, 696, 774, 776
FaqI GGGAC 1 cut(s) 704
FatI CATG 3 cut(s) 57, 99, 125
FauI CCCGC 2 cut(s) 34, 774
Fnu4HI GCNGC 3 cut(s) 91, 147, 198
FokI GGATG 3 cut(s) 74, 335, 506
FriOI GRGCYC 1 cut(s) 771
Fsp4HI GCNGC 3 cut(s) 91, 147, 198
FspBI CTAG 1 cut(s) 615
GluI GCNGC 3 cut(s) 91, 147, 198
HaeIII GGCC 3 cut(s) 5, 104, 557
HapII CCGG 3 cut(s) 32, 383, 401
Hin1II CATG 3 cut(s) 61, 103, 129
HindIII AAGCTT 2 cut(s) 246, 863
HinfI GANTC 4 cut(s) 65, 209, 598, 755
HpaII CCGG 3 cut(s) 32, 383, 401
HphI GGTGA 3 cut(s) 46, 157, 397
Hpy188I TCNGA 3 cut(s) 176, 664, 804
Hpy188III TCNNGA 2 cut(s) 53, 401
HpyAV CCTTC 5 cut(s) 168, 178, 301, 406, 482
HpyCH4III ACNGT 4 cut(s) 79, 571, 584, 637
HpyCH4V TGCA 7 cut(s) 93, 120, 149, 200, 331, 530, 818
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 3 cut(s) 71, 453, 759
Hsp92II CATG 3 cut(s) 61, 103, 129
Kpn2I TCCGGA 1 cut(s) 400
Kzo9I GATC 1 cut(s) 762
LmnI GCTCC 1 cut(s) 611
LpnPI CCDG 5 cut(s) 45, 396, 414, 441, 573
Lsp1109I GCAGC 3 cut(s) 77, 133, 184
LweI GCATC 4 cut(s) 102, 129, 313, 538
MaeI CTAG 1 cut(s) 615
MaeIII GTNAC 7 cut(s) 108, 430, 448, 505, 605, 701, 791
MalI GATC 1 cut(s) 764
MboI GATC 1 cut(s) 762
MboII GAAGA 3 cut(s) 400, 694, 797
MhlI GDGCHC 1 cut(s) 771
MlsI TGGCCA 2 cut(s) 5, 557
MluCI AATT 1 cut(s) 542
MluNI TGGCCA 2 cut(s) 5, 557
MlyI GAGTC 2 cut(s) 203, 592
MnlI CCTC 7 cut(s) 136, 173, 182, 223, 292, 328, 569
Mox20I TGGCCA 2 cut(s) 5, 557
MroI TCCGGA 1 cut(s) 400
MscI TGGCCA 2 cut(s) 5, 557
MslI CAYNNNNRTG 2 cut(s) 98, 115
Msp20I TGGCCA 2 cut(s) 5, 557
MspI CCGG 3 cut(s) 32, 383, 401
Mva1269I GAATGC 1 cut(s) 43
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 1 cut(s) 762
NlaIII CATG 3 cut(s) 61, 103, 129
NlaIV GGNNCC 1 cut(s) 105
NmuCI GTSAC 4 cut(s) 108, 430, 448, 791
OliI CACNNNNGTG 1 cut(s) 115
PcsI WCGNNNNNNNCGW 1 cut(s) 138
PctI GAATGC 1 cut(s) 43
PfeI GAWTC 2 cut(s) 65, 755
PkrI GCNGC 3 cut(s) 92, 148, 199
PleI GAGTC 2 cut(s) 203, 592
PpsI GAGTC 2 cut(s) 203, 592
PspEI GGTNACC 1 cut(s) 701
PspN4I GGNNCC 1 cut(s) 105
PspPI GGNCC 1 cut(s) 103
RsaI GTAC 4 cut(s) 25, 76, 306, 586
RsaNI GTAC 4 cut(s) 24, 75, 305, 585
RseI CAYNNNNRTG 2 cut(s) 98, 115
SatI GCNGC 3 cut(s) 91, 147, 198
Sau3AI GATC 1 cut(s) 762
Sau96I GGNCC 1 cut(s) 103
ScaI AGTACT 3 cut(s) 25, 76, 586
SchI GAGTC 2 cut(s) 203, 592
SduI GDGCHC 1 cut(s) 771
SfaNI GCATC 4 cut(s) 102, 129, 313, 538
SmiMI CAYNNNNRTG 2 cut(s) 98, 115
SmlI CTYRAG 1 cut(s) 216
SmoI CTYRAG 1 cut(s) 216
Sse9I AATT 1 cut(s) 542
SsiI CCGC 2 cut(s) 41, 781
SspMI CTAG 1 cut(s) 615
TaaI ACNGT 4 cut(s) 79, 571, 584, 637
TaqI TCGA 3 cut(s) 546, 626, 765
TasI AATT 1 cut(s) 542
TatI WGTACW 3 cut(s) 23, 74, 584
TfiI GAWTC 2 cut(s) 65, 755
TscAI CASTG 1 cut(s) 457
TseFI GTSAC 4 cut(s) 108, 430, 448, 791
TseI GCWGC 3 cut(s) 90, 146, 197
Tsp45I GTSAC 4 cut(s) 108, 430, 448, 791
TspDTI ATGAA 2 cut(s) 508, 555
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 1 cut(s) 457
XapI RAATTY 1 cut(s) 542
XspI CTAG 1 cut(s) 615
ZrmI AGTACT 3 cut(s) 25, 76, 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.