RchiOBHm_Chr2g0115231

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018568
Physical Location & Seq
Forward (+)
27272769 .. 27274269
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48842

Sequence Viewer

Length: 711 bp
ATGTATAAGTATTCTATGCTTCATTTATATAATAATTGGGACACTTACATCACTGAAGACGATTTCAAGTACATGTCATCAAATGGCTTGAATGCGGTGAGGATCCTAGTTGGGTGGTGGATTGCATATAAAGGTCAAACACGGACATCGCCTTTTGTTAGGGGAGCTGGAGACTGTTTAAAAGCCTTGGACAATGCTTTAACTTGGGCAAAGTTCACAGTATTCTTTGAAGATATGTGCAGAAACACTGGAATGAAGGTGATTATAGACCTACATGCTGTTGAAGGTTCCCAAAATGGCAATGATCACAATGGAACAAGAGATGGCTCTCAGGAATGGGAAGATAAATACATAGACAAGACTGTGGAAGTTATAGAATTCTTAGCAGCAAGGTTAATTTTATGTCATGCACTACATGCGCATGTATATGTATATCATGCATCTCATCTCATTTGCCCAAGGCATTACTACAGCTTATATTTAGACAAGTTCAACGGCTTCAGTGTGCAACAAAATATTAATTTCATCTACAACCAACGAGGTTCTGATCTCACTGCTGTGATCAATTTTAATGGACCCCTCACTTTTGTTGGGGAATGGATTGCAGGATGGGCAATAAATGGAGCATCTATGCAAGACTACCAGAGATTTGCCAAAGCACAACTAGATGTTTACGGCCGTGCTACTTGGATGGGCATACTGGTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase

Protein Analysis

236

Amino Acids

27.16

Weight (kDa)

6.29

Isoelectric Point (pI)

22.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018568)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115231
rosa_samantha Rh2AG241900 Rh2AG253100 Rh2BG263000 Rh2BG263300 Rh2CG257900 Rh2CG258000 Rh2DG249800 Rh2DG261300
rosa_wichuraiana Rw2G019780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 709
Acc16I TGCGCA 1 cut(s) 420
AciI CCGC 1 cut(s) 95
AclWI GGATC 2 cut(s) 97, 110
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 1 cut(s) 377
AcuI CTGAAG 2 cut(s) 75, 484
AfaI GTAC 1 cut(s) 71
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 5 cut(s) 67, 91, 230, 284, 493
AleI CACNNNNGTG 1 cut(s) 557
AluBI AGCT 2 cut(s) 167, 474
AluI AGCT 2 cut(s) 167, 474
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 2 cut(s) 97, 110
AoxI GGCC 1 cut(s) 676
ApeKI GCWGC 1 cut(s) 386
ApoI RAATTY 1 cut(s) 377
AseI ATTAAT 1 cut(s) 519
AspLEI GCGC 1 cut(s) 421
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 109, 271
AvaII GGWCC 1 cut(s) 575
BamHI GGATCC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 1 cut(s) 398
BccI CCATC 3 cut(s) 317, 603, 685
BceAI ACGGC 3 cut(s) 511, 663, 691
BclI TGATCA 2 cut(s) 304, 561
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 2 cut(s) 107, 665
BfmI CTRYAG 1 cut(s) 469
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme18I GGWCC 1 cut(s) 575
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 3 cut(s) 104, 289, 577
BmsI GCATC 2 cut(s) 449, 635
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 186, 458
Bse1I ACTGG 2 cut(s) 253, 705
Bse3DI GCAATG 1 cut(s) 307
BseDI CCNNGG 2 cut(s) 186, 458
BseGI GGATG 2 cut(s) 614, 696
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 344
BseNI ACTGG 2 cut(s) 253, 705
BseX3I CGGCCG 1 cut(s) 676
BseXI GCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 259
Bsh1285I CGRYCG 1 cut(s) 679
BshFI GGCC 1 cut(s) 678
BsiEI CGRYCG 1 cut(s) 679
BslFI GGGAC 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 165
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 97
BsnI GGCC 1 cut(s) 678
Bsp143I GATC 4 cut(s) 102, 304, 547, 561
BspACI CCGC 1 cut(s) 95
BspANI GGCC 1 cut(s) 678
BspCNI CTCAG 1 cut(s) 343
BspLI GGNNCC 3 cut(s) 104, 289, 577
BspPI GGATC 2 cut(s) 97, 110
BsrDI GCAATG 1 cut(s) 307
BsrI ACTGG 2 cut(s) 253, 705
BssECI CCNNGG 2 cut(s) 186, 458
BssMI GATC 4 cut(s) 102, 304, 547, 561
BssT1I CCWWGG 2 cut(s) 186, 458
Bst4CI ACNGT 3 cut(s) 176, 220, 364
BstAPI GCANNNNNTGC 1 cut(s) 416
BstDEI CTNAG 2 cut(s) 330, 382
BstF5I GGATG 2 cut(s) 614, 696
BstHHI GCGC 1 cut(s) 421
BstKTI GATC 4 cut(s) 105, 307, 550, 564
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 4 cut(s) 102, 304, 547, 561
BstMCI CGRYCG 1 cut(s) 679
BstMWI GCNNNNNNNGC 2 cut(s) 416, 611
BstNSI RCATGY 4 cut(s) 76, 278, 419, 425
BstSFI CTRYAG 1 cut(s) 469
BstV1I GCAGC 1 cut(s) 398
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BstZI CGGCCG 1 cut(s) 676
BsuRI GGCC 1 cut(s) 678
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 614, 696
BtsI GCAGTG 1 cut(s) 552
BtsIMutI CAGTG 4 cut(s) 51, 246, 508, 552
CfoI GCGC 1 cut(s) 421
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 1 cut(s) 70
CviAII CATG 6 cut(s) 73, 275, 407, 416, 422, 437
CviJI RGCY 7 cut(s) 87, 167, 185, 327, 474, 498, 678
CviKI_1 RGCY 7 cut(s) 87, 167, 185, 327, 474, 498, 678
CviQI GTAC 1 cut(s) 70
DdeI CTNAG 2 cut(s) 330, 382
DpnI GATC 4 cut(s) 104, 306, 549, 563
DpnII GATC 4 cut(s) 102, 304, 547, 561
DraI TTTAAA 1 cut(s) 180
EaeI YGGCCR 1 cut(s) 676
EagI CGGCCG 1 cut(s) 676
EclXI CGGCCG 1 cut(s) 676
Eco130I CCWWGG 2 cut(s) 186, 458
Eco47I GGWCC 1 cut(s) 575
Eco52I CGGCCG 1 cut(s) 676
Eco57I CTGAAG 2 cut(s) 75, 484
EcoRI GAATTC 1 cut(s) 377
EcoT14I CCWWGG 2 cut(s) 186, 458
EcoT22I ATGCAT 1 cut(s) 442
ErhI CCWWGG 2 cut(s) 186, 458
FaeI CATG 6 cut(s) 76, 278, 410, 419, 425, 440
FaqI GGGAC 1 cut(s) 53
FatI CATG 6 cut(s) 72, 274, 406, 415, 421, 436
FbaI TGATCA 2 cut(s) 304, 561
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 2 cut(s) 621, 703
Fsp4HI GCNGC 1 cut(s) 387
FspAI RTGCGCAY 1 cut(s) 420
FspBI CTAG 2 cut(s) 107, 665
FspI TGCGCA 1 cut(s) 420
GlaI GCGC 1 cut(s) 420
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 189
HaeIII GGCC 1 cut(s) 678
HhaI GCGC 1 cut(s) 421
Hin1II CATG 6 cut(s) 76, 278, 410, 419, 425, 440
Hin6I GCGC 1 cut(s) 419
HinP1I GCGC 1 cut(s) 419
HphI GGTGA 2 cut(s) 109, 271
Hpy166II GTNNAC 2 cut(s) 216, 673
Hpy188I TCNGA 1 cut(s) 547
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 216, 673
HpyAV CCTTC 2 cut(s) 250, 278
HpyCH4III ACNGT 3 cut(s) 176, 220, 364
HpyCH4V TGCA 7 cut(s) 125, 240, 410, 440, 508, 605, 634
HpyF10VI GCNNNNNNNGC 2 cut(s) 416, 611
HpyF3I CTNAG 2 cut(s) 330, 382
Hsp92II CATG 6 cut(s) 76, 278, 410, 419, 425, 440
HspAI GCGC 1 cut(s) 419
Ksp22I TGATCA 2 cut(s) 304, 561
Kzo9I GATC 4 cut(s) 102, 304, 547, 561
LmnI GCTCC 2 cut(s) 164, 623
LpnPI CCDG 6 cut(s) 153, 234, 317, 591, 656, 686
Lsp1109I GCAGC 1 cut(s) 398
LweI GCATC 2 cut(s) 449, 635
MaeI CTAG 2 cut(s) 107, 665
MalI GATC 4 cut(s) 104, 306, 549, 563
MboI GATC 4 cut(s) 102, 304, 547, 561
MboII GAAGA 3 cut(s) 68, 242, 353
MflI RGATCY 1 cut(s) 102
MluCI AATT 5 cut(s) 34, 377, 396, 520, 565
MnlI CCTC 3 cut(s) 93, 533, 590
Mph1103I ATGCAT 1 cut(s) 442
MseI TTAA 5 cut(s) 179, 200, 395, 519, 570
MslI CAYNNNNRTG 4 cut(s) 251, 420, 426, 557
Mva1269I GAATGC 1 cut(s) 97
MwoI GCNNNNNNNGC 2 cut(s) 416, 611
NdeII GATC 4 cut(s) 102, 304, 547, 561
NlaIII CATG 6 cut(s) 76, 278, 410, 419, 425, 440
NlaIV GGNNCC 3 cut(s) 104, 289, 577
NsbI TGCGCA 1 cut(s) 420
NsiI ATGCAT 1 cut(s) 442
NspI RCATGY 4 cut(s) 76, 278, 419, 425
OliI CACNNNNGTG 1 cut(s) 557
PciI ACATGT 1 cut(s) 72
PctI GAATGC 1 cut(s) 97
PkrI GCNGC 1 cut(s) 388
PscI ACATGT 1 cut(s) 72
PshBI ATTAAT 1 cut(s) 519
PsiI TTATAA 1 cut(s) 709
PspN4I GGNNCC 3 cut(s) 104, 289, 577
PspPI GGNCC 1 cut(s) 575
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 1 cut(s) 71
RsaNI GTAC 1 cut(s) 70
RseI CAYNNNNRTG 4 cut(s) 251, 420, 426, 557
SaqAI TTAA 5 cut(s) 179, 200, 395, 519, 570
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 4 cut(s) 102, 304, 547, 561
Sau96I GGNCC 1 cut(s) 575
SetI ASST 8 cut(s) 136, 169, 261, 273, 289, 395, 476, 544
SfaNI GCATC 2 cut(s) 449, 635
SfcI CTRYAG 1 cut(s) 469
SinI GGWCC 1 cut(s) 575
SmiMI CAYNNNNRTG 4 cut(s) 251, 420, 426, 557
Sse9I AATT 5 cut(s) 34, 377, 396, 520, 565
SsiI CCGC 1 cut(s) 95
SspI AATATT 1 cut(s) 517
SspMI CTAG 2 cut(s) 107, 665
StyI CCWWGG 2 cut(s) 186, 458
TaaI ACNGT 3 cut(s) 176, 220, 364
TasI AATT 5 cut(s) 34, 377, 396, 520, 565
TatI WGTACW 1 cut(s) 69
Tru1I TTAA 5 cut(s) 179, 200, 395, 519, 570
Tru9I TTAA 5 cut(s) 179, 200, 395, 519, 570
TscAI CASTG 4 cut(s) 58, 253, 508, 559
TseI GCWGC 1 cut(s) 386
TspDTI ATGAA 3 cut(s) 11, 269, 514
TspGWI ACGGA 1 cut(s) 157
TspRI CASTG 4 cut(s) 58, 253, 508, 559
VpaK11BI GGWCC 1 cut(s) 575
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 1 cut(s) 377
XceI RCATGY 4 cut(s) 76, 278, 419, 425
XspI CTAG 2 cut(s) 107, 665
Zsp2I ATGCAT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.