Rh2BG263300

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018568
Physical Location & Seq
Forward (+)
28167812 .. 28168423
612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG263300.1

Sequence Viewer

Length: 381 bp
ATGTGCAGAAACACTGGAATGAAGGTGAATATAGACCTACATGCTGTTGAAGGTTCCCAAAACGGCAATGATCACAATGGAACAAGAGATGGCTCTCAGGAATGGGGAGATAAATACATAGACAAGACTGTGGAAGTTATAGAATTCTTAGCAGCAAGATTGGCTGGTAACCCAGCTTTTGCAGCAATTGAGTTGCTGAATGAGCCTCTAGCTCCATGTGTGACTATATACAACCTCAAAAAGTATTACAAAGCAGGATATGATGCAGTAAGGAAGTACACTTCAAGTGCCTATGTGATCCTATCAAACCGATTGGGACGTGCTGATCCACATGAGCTCCTCTCATTTGCCCAAGGCATTACTACAGCTTATATTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase

Protein Analysis

126

Amino Acids

13.94

Weight (kDa)

6.27

Isoelectric Point (pI)

23.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cellulase PF00150 2 - 103 5e-08 Cellulase (glycosyl hydrolase family 5)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018568)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115231
rosa_samantha Rh2AG241900 Rh2AG253100 Rh2BG263000 Rh2BG263300 Rh2CG257900 Rh2CG258000 Rh2DG249800 Rh2DG261300
rosa_wichuraiana Rw2G019780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 292, 320
AcsI RAATTY 1 cut(s) 143
AfaI GTAC 1 cut(s) 278
AgsI TTSAA 2 cut(s) 50, 285
AjiI CACGTC 1 cut(s) 320
AluBI AGCT 4 cut(s) 176, 212, 337, 368
AluI AGCT 4 cut(s) 176, 212, 337, 368
Alw21I GWGCWC 1 cut(s) 339
AlwI GGATC 2 cut(s) 292, 320
ApeKI GCWGC 2 cut(s) 152, 182
ApoI RAATTY 1 cut(s) 143
AsuHPI GGTGA 1 cut(s) 37
BanII GRGCYC 1 cut(s) 339
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 2 cut(s) 164, 194
BccI CCATC 1 cut(s) 83
BceAI ACGGC 1 cut(s) 79
BclI TGATCA 1 cut(s) 70
BfaI CTAG 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 2 cut(s) 153, 183
BlsI GCNGC 2 cut(s) 154, 184
BmgBI CACGTC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 55
BmsI GCATC 1 cut(s) 253
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BsaJI CCNNGG 1 cut(s) 352
BsaXI ACNNNNNCTCC 2 cut(s) 321, 351
Bse1I ACTGG 1 cut(s) 19
Bse3DI GCAATG 1 cut(s) 73
BseDI CCNNGG 1 cut(s) 352
BseMI GCAATG 1 cut(s) 73
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 19
BseRI GAGGAG 1 cut(s) 329
BseXI GCAGC 2 cut(s) 164, 194
BseYI CCCAGC 1 cut(s) 172
BsgI GTGCAG 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 339
BslFI GGGAC 1 cut(s) 330
BsmFI GGGAC 1 cut(s) 330
Bsp1286I GDGCHC 1 cut(s) 339
Bsp143I GATC 3 cut(s) 70, 297, 325
BspCNI CTCAG 1 cut(s) 109
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 2 cut(s) 292, 320
BsrDI GCAATG 1 cut(s) 73
BsrI ACTGG 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 352
BssMI GATC 3 cut(s) 70, 297, 325
BssT1I CCWWGG 1 cut(s) 352
Bst4CI ACNGT 1 cut(s) 130
BstDEI CTNAG 2 cut(s) 96, 148
BstEII GGTNACC 1 cut(s) 167
BstKTI GATC 3 cut(s) 73, 300, 328
BstMBI GATC 3 cut(s) 70, 297, 325
BstMWI GCNNNNNNNGC 3 cut(s) 161, 182, 202
BstNSI RCATGY 1 cut(s) 44
BstPI GGTNACC 1 cut(s) 167
BstSFI CTRYAG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 164, 194
BtrI CACGTC 1 cut(s) 320
BtsIMutI CAGTG 1 cut(s) 12
Csp6I GTAC 1 cut(s) 277
CviAII CATG 3 cut(s) 41, 216, 332
CviJI RGCY 7 cut(s) 93, 164, 176, 205, 212, 337, 368
CviKI_1 RGCY 7 cut(s) 93, 164, 176, 205, 212, 337, 368
CviQI GTAC 1 cut(s) 277
DdeI CTNAG 2 cut(s) 96, 148
DpnI GATC 3 cut(s) 72, 299, 327
DpnII GATC 3 cut(s) 70, 297, 325
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 339
Eco53kI GAGCTC 1 cut(s) 337
Eco91I GGTNACC 1 cut(s) 167
EcoICRI GAGCTC 1 cut(s) 337
EcoO65I GGTNACC 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 352
FaeI CATG 3 cut(s) 44, 219, 335
FaqI GGGAC 1 cut(s) 330
FatI CATG 3 cut(s) 40, 215, 331
FbaI TGATCA 1 cut(s) 70
Fnu4HI GCNGC 2 cut(s) 153, 183
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 2 cut(s) 153, 183
FspBI CTAG 1 cut(s) 209
GluI GCNGC 2 cut(s) 153, 183
GsaI CCCAGC 1 cut(s) 176
Hin1II CATG 3 cut(s) 44, 219, 335
HphI GGTGA 1 cut(s) 37
Hpy166II GTNNAC 1 cut(s) 279
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 1 cut(s) 279
HpyAV CCTTC 2 cut(s) 16, 44
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4IV ACGT 1 cut(s) 319
HpyCH4V TGCA 3 cut(s) 6, 182, 266
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 182, 202
HpyF3I CTNAG 2 cut(s) 96, 148
HpySE526I ACGT 1 cut(s) 319
Hsp92II CATG 3 cut(s) 44, 219, 335
Ksp22I TGATCA 1 cut(s) 70
Kzo9I GATC 3 cut(s) 70, 297, 325
LmnI GCTCC 2 cut(s) 217, 342
LpnPI CCDG 4 cut(s) 83, 150, 186, 240
Lsp1109I GCAGC 2 cut(s) 164, 194
LweI GCATC 1 cut(s) 253
MaeI CTAG 1 cut(s) 209
MaeII ACGT 1 cut(s) 319
MaeIII GTNAC 2 cut(s) 167, 220
MalI GATC 3 cut(s) 72, 299, 327
MboI GATC 3 cut(s) 70, 297, 325
MfeI CAATTG 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 339
MluCI AATT 2 cut(s) 143, 186
MnlI CCTC 3 cut(s) 216, 245, 350
MslI CAYNNNNRTG 1 cut(s) 17
MunI CAATTG 1 cut(s) 186
MwoI GCNNNNNNNGC 3 cut(s) 161, 182, 202
NdeII GATC 3 cut(s) 70, 297, 325
NlaIII CATG 3 cut(s) 44, 219, 335
NlaIV GGNNCC 1 cut(s) 55
NmuCI GTSAC 1 cut(s) 220
NspI RCATGY 1 cut(s) 44
PkrI GCNGC 2 cut(s) 154, 184
Psp124BI GAGCTC 1 cut(s) 339
PspEI GGTNACC 1 cut(s) 167
PspFI CCCAGC 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 55
RsaI GTAC 1 cut(s) 278
RsaNI GTAC 1 cut(s) 277
RseI CAYNNNNRTG 1 cut(s) 17
SacI GAGCTC 1 cut(s) 339
SatI GCNGC 2 cut(s) 153, 183
Sau3AI GATC 3 cut(s) 70, 297, 325
SduI GDGCHC 1 cut(s) 339
SetI ASST 9 cut(s) 27, 39, 55, 178, 214, 237, 322, 339, 370
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 17
Sse9I AATT 2 cut(s) 143, 186
SspMI CTAG 1 cut(s) 209
SstI GAGCTC 1 cut(s) 339
StyI CCWWGG 1 cut(s) 352
TaaI ACNGT 1 cut(s) 130
TaiI ACGT 1 cut(s) 322
TasI AATT 2 cut(s) 143, 186
TatI WGTACW 1 cut(s) 276
TscAI CASTG 1 cut(s) 19
TseFI GTSAC 1 cut(s) 220
TseI GCWGC 2 cut(s) 152, 182
Tsp45I GTSAC 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 35
TspRI CASTG 1 cut(s) 19
XapI RAATTY 1 cut(s) 143
XceI RCATGY 1 cut(s) 44
XspI CTAG 1 cut(s) 209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.