Rh2CG257900

Belongs to the glycosyl hydrolase 5 (cellulase A) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0018568
Physical Location & Seq
Forward (+)
27518381 .. 27519587
1207 bp
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UTR
Exon/CDS
Intron
Rh2CG257900.1

Sequence Viewer

Length: 480 bp
ATGGGACTCGAGTGCAATTTAAGTCCACAAAAATTGCAGAAGTATTTGTCCGTGAATCAAGGTGGTGGTTCCACTATTCTTGTTGACAAGCTTGAACCCAGTGCTTGGAAACTTTCAAGCTGTGGAGGACCAATCAATCATCTTTCAACTTCAGAACCTATGATAGATCAACCTTTTGTGGGAAAGTCAAAGGTTAGCAGCAAGGTCTTAGTAAAGAGAACCATGCAAGAACTTGGTGACATCGAGATATTTCAGATTGAGACTGAAATTGAGGAACCACAAGATGAAAAAACCTTTCGAGTATGCATTCGATCATCCAATGACGATTTCAAGTACATGTCATCAAATGGCTTGAATGCGGTGAGGATCCTAGTTGGGTGGTGGATTGCATATAAAGGTCAAACACGGACATCGCCTTTTGTTAGGGGAGCTGGAGACTGTTTAAAAGCCTTGGACAATGCTTTAACTTGGGCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cellulase

Protein Analysis

159

Amino Acids

17.64

Weight (kDa)

7.62

Isoelectric Point (pI)

59.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7910 PF25490 2 - 112 3.6e-06 Domain of unknown function (DUF7910)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018568)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr2g0115231
rosa_samantha Rh2AG241900 Rh2AG253100 Rh2BG263000 Rh2BG263300 Rh2CG257900 Rh2CG258000 Rh2DG249800 Rh2DG261300
rosa_wichuraiana Rw2G019780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 105
AciI CCGC 1 cut(s) 359
AclWI GGATC 2 cut(s) 361, 374
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 2 cut(s) 105, 179
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 5 cut(s) 95, 117, 147, 331, 355
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 3 cut(s) 91, 120, 431
AluI AGCT 3 cut(s) 91, 120, 431
Alw26I GTCTC 2 cut(s) 254, 429
AlwI GGATC 2 cut(s) 361, 374
Ama87I CYCGRG 1 cut(s) 8
ApeKI GCWGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 248, 373
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 128
BamHI GGATCC 1 cut(s) 366
BbvI GCAGC 1 cut(s) 210
BcoDI GTCTC 2 cut(s) 254, 429
BfaI CTAG 1 cut(s) 371
BisI GCNGC 1 cut(s) 199
BlsI GCNGC 1 cut(s) 200
Bme18I GGWCC 1 cut(s) 128
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 128
BmiI GGNNCC 3 cut(s) 70, 276, 368
BmrI ACTGGG 1 cut(s) 93
BmuI ACTGGG 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 453
BsaJI CCNNGG 1 cut(s) 450
Bsc4I CCNNNNNNNGG 2 cut(s) 105, 179
Bse1I ACTGG 1 cut(s) 99
BseDI CCNNGG 1 cut(s) 450
BseGI GGATG 1 cut(s) 314
BseLI CCNNNNNNNGG 2 cut(s) 105, 179
BseNI ACTGG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 8
BslFI GGGAC 1 cut(s) 18
BslI CCNNNNNNNGG 2 cut(s) 105, 179
BsmAI GTCTC 2 cut(s) 254, 429
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 2 cut(s) 306, 361
BsoBI CYCGRG 1 cut(s) 8
Bsp143I GATC 3 cut(s) 166, 311, 366
BspACI CCGC 1 cut(s) 359
BspLI GGNNCC 3 cut(s) 70, 276, 368
BspPI GGATC 2 cut(s) 361, 374
BsrI ACTGG 1 cut(s) 99
BssECI CCNNGG 1 cut(s) 450
BssMI GATC 3 cut(s) 166, 311, 366
BssT1I CCWWGG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 440
BstDEI CTNAG 1 cut(s) 208
BstF5I GGATG 1 cut(s) 314
BstKTI GATC 3 cut(s) 169, 314, 369
BstMAI GTCTC 2 cut(s) 254, 429
BstMBI GATC 3 cut(s) 166, 311, 366
BstNSI RCATGY 1 cut(s) 340
BstV1I GCAGC 1 cut(s) 210
BstX2I RGATCY 1 cut(s) 366
BstYI RGATCY 1 cut(s) 366
BtgZI GCGATG 1 cut(s) 396
BtsCI GGATG 1 cut(s) 314
BtsIMutI CAGTG 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 334
CspCI CAANNNNNGTGG 4 cut(s) 15, 50, 61, 96
CviAII CATG 2 cut(s) 223, 337
CviJI RGCY 5 cut(s) 91, 120, 351, 431, 449
CviKI_1 RGCY 5 cut(s) 91, 120, 351, 431, 449
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 1 cut(s) 208
DpnI GATC 3 cut(s) 168, 313, 368
DpnII GATC 3 cut(s) 166, 311, 366
DraI TTTAAA 1 cut(s) 444
Eco130I CCWWGG 1 cut(s) 450
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 450
EcoT22I ATGCAT 1 cut(s) 308
ErhI CCWWGG 1 cut(s) 450
FaeI CATG 2 cut(s) 226, 340
FaiI YATR 6 cut(s) 161, 224, 304, 338, 391, 393
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 222, 336
Fnu4HI GCNGC 1 cut(s) 199
FokI GGATG 1 cut(s) 301
Fsp4HI GCNGC 1 cut(s) 199
FspBI CTAG 1 cut(s) 371
GluI GCNGC 1 cut(s) 199
GsuI CTGGAG 1 cut(s) 453
Hin1II CATG 2 cut(s) 226, 340
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 1 cut(s) 89
HinfI GANTC 2 cut(s) 6, 55
HphI GGTGA 2 cut(s) 248, 373
Hpy166II GTNNAC 2 cut(s) 26, 85
Hpy188I TCNGA 2 cut(s) 154, 255
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 2 cut(s) 26, 85
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4V TGCA 5 cut(s) 15, 37, 226, 306, 389
HpyF3I CTNAG 1 cut(s) 208
Hsp92II CATG 2 cut(s) 226, 340
Kzo9I GATC 3 cut(s) 166, 311, 366
LmnI GCTCC 1 cut(s) 428
LpnPI CCDG 2 cut(s) 112, 417
Lsp1109I GCAGC 1 cut(s) 210
MaeI CTAG 1 cut(s) 371
MaeIII GTNAC 1 cut(s) 236
MalI GATC 3 cut(s) 168, 313, 368
MboI GATC 3 cut(s) 166, 311, 366
MflI RGATCY 1 cut(s) 366
MluCI AATT 3 cut(s) 16, 32, 267
MnlI CCTC 3 cut(s) 119, 265, 357
Mph1103I ATGCAT 1 cut(s) 308
MseI TTAA 3 cut(s) 20, 443, 464
Mva1269I GAATGC 2 cut(s) 306, 361
NdeII GATC 3 cut(s) 166, 311, 366
NlaIII CATG 2 cut(s) 226, 340
NlaIV GGNNCC 3 cut(s) 70, 276, 368
NmuCI GTSAC 1 cut(s) 236
NsiI ATGCAT 1 cut(s) 308
NspI RCATGY 1 cut(s) 340
PaeR7I CTCGAG 1 cut(s) 8
PciI ACATGT 1 cut(s) 336
PctI GAATGC 2 cut(s) 306, 361
PfeI GAWTC 1 cut(s) 55
PflMI CCANNNNNTGG 1 cut(s) 105
PkrI GCNGC 1 cut(s) 200
PscI ACATGT 1 cut(s) 336
PspN4I GGNNCC 3 cut(s) 70, 276, 368
PspPI GGNCC 1 cut(s) 128
PspXI VCTCGAGB 1 cut(s) 8
PsuI RGATCY 1 cut(s) 366
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SaqAI TTAA 3 cut(s) 20, 443, 464
SatI GCNGC 1 cut(s) 199
Sau3AI GATC 3 cut(s) 166, 311, 366
Sau96I GGNCC 1 cut(s) 128
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 128
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 8
SmoI CTYRAG 1 cut(s) 8
Sse9I AATT 3 cut(s) 16, 32, 267
SsiI CCGC 1 cut(s) 359
SspMI CTAG 1 cut(s) 371
StyI CCWWGG 1 cut(s) 450
TaaI ACNGT 1 cut(s) 440
TaqI TCGA 4 cut(s) 9, 243, 298, 310
TasI AATT 3 cut(s) 16, 32, 267
TatI WGTACW 1 cut(s) 333
TfiI GAWTC 1 cut(s) 55
Tru1I TTAA 3 cut(s) 20, 443, 464
Tru9I TTAA 3 cut(s) 20, 443, 464
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 236
TseI GCWGC 1 cut(s) 198
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 300
TspGWI ACGGA 2 cut(s) 40, 421
TspRI CASTG 1 cut(s) 106
Van91I CCANNNNNTGG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 128
XceI RCATGY 1 cut(s) 340
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.