RchiOBHm_Chr2g0139591

VHS domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0008809
Physical Location & Seq
Forward (+)
57135691 .. 57137589
1899 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51012

Sequence Viewer

Length: 996 bp
ATGGATTCGAGTCGGAGAGCTGTGGAGTCGTACTGGAGGTCTCGGATGGTCGATGCGGCTACGTCGGACGAAGACAAGGTCGCTCCAGTTTACAAATTGGAGGAGATTTGCCAGCTGCTCAGGTCCTCGCCTGGCAGTATCGTCAAGGAGATGTCTGAGTTTGTGTTCAAGCGTCTCGACCATAAAAGCCCTATTGTTAAACAGAAGGCTCTGCGATTGATAAAATATGCGGTGGGAAAGTCTGGTGTAGAGTTCAGGAGAGAAATGCAGAGACATTCAGTGGCTGTGCGCCAGTTATTTCACTACAAGGGACAGCCAGATCCTTTGAAAGGAGATGCACTTAATAAGGCTGTGCGGGACACTGCTCAGGAGGCTATTGCTGCCATCTTTTCAGAGGAAAGTAAGCCCGCACCTGCAGAAGATCTTAACAGACGAATTCAAGGTTTTGGGAATACAAACTATGAAATGCCAACAGAGGAAAAGAAATCGTTTATTAGTGAGGTAGTTGGTCTTGGAAGTGCATCTATCAAGCAGGGGTTTAGTACTTTCACCCAAGGTCAGTCGTTTAAAAAGAATGACAATGGAAGCTACAAGAGTCCTAATCTTCATAGGTCATTGACTATGGAAGGGAAAGCAGATAAATATGAACCATTTCAGTATCCCAATCAGACTCAGAGTAGTTTTGAAAATTCAAAGAATGTGGCTAATGGACCCTGGGGTGAGGACTCAAGGTCAACCAAGCCTGAACTAACTAATGGAGATTCTAGTTCAAGTTATAGAGAGAGTAAGACCCGTGAGGAGAGGTTGTTGGAGACCATTGTAACGTCAGGTGGTGTACGCTTACAACCCACTCGAGATGCTATTCAGGTTTTCCTTGCAGAGGCGGCGAAATTGGATGCAGTAGCATTAAGTCTTGCCCTTGAATCAAAGCTCCAATCTCCATTGTGGCAGGCATGCTTTTTATACTTATTTTATGGCTTTAATTCTTTTGGATAG

Protein Analysis

331

Amino Acids

37.14

Weight (kDa)

8.95

Isoelectric Point (pI)

56.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ENTH PF01417 16 - 128 7.1e-07 ENTH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 421
Acc36I ACCTGC 1 cut(s) 421
AciI CCGC 5 cut(s) 56, 230, 355, 408, 884
AclWI GGATC 1 cut(s) 314
AcsI RAATTY 2 cut(s) 435, 688
AfaI GTAC 3 cut(s) 32, 544, 837
AfiI CCNNNNNNNGG 2 cut(s) 329, 880
AgsI TTSAA 7 cut(s) 169, 328, 440, 686, 693, 771, 923
AhdI GACNNNNNGTC 1 cut(s) 730
AjnI CCWGG 2 cut(s) 130, 713
AluBI AGCT 4 cut(s) 20, 115, 588, 931
AluI AGCT 4 cut(s) 20, 115, 588, 931
Alw26I GTCTC 4 cut(s) 45, 179, 265, 806
AlwI GGATC 1 cut(s) 314
AlwNI CAGNNNCTG 1 cut(s) 284
Ama87I CYCGRG 1 cut(s) 852
ApeKI GCWGC 2 cut(s) 115, 380
ApoI RAATTY 2 cut(s) 435, 688
ArsI GACNNNNNNTTYG 2 cut(s) 63, 95
Asp700I GAANNNNTTC 1 cut(s) 651
AspLEI GCGC 1 cut(s) 291
AspS9I GGNCC 2 cut(s) 123, 710
AsuHPI GGTGA 2 cut(s) 541, 731
AvaI CYCGRG 1 cut(s) 852
AvaII GGWCC 2 cut(s) 123, 710
BbsI GAAGAC 1 cut(s) 78
BbvI GCAGC 2 cut(s) 102, 367
BccI CCATC 2 cut(s) 40, 392
BciT130I CCWGG 2 cut(s) 132, 715
BciVI GTATCC 1 cut(s) 669
BcoDI GTCTC 4 cut(s) 45, 179, 265, 806
BfaI CTAG 1 cut(s) 765
BfmI CTRYAG 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 421
BfuI GTATCC 1 cut(s) 669
BglII AGATCT 1 cut(s) 421
BisI GCNGC 4 cut(s) 57, 116, 381, 885
BlsI GCNGC 4 cut(s) 58, 117, 382, 886
BmcAI AGTACT 1 cut(s) 544
Bme1390I CCNGG 2 cut(s) 132, 715
Bme18I GGWCC 2 cut(s) 123, 710
BmeRI GACNNNNNGTC 1 cut(s) 730
BmeT110I CYCGRG 1 cut(s) 852
BmgT120I GGNCC 2 cut(s) 123, 710
BmiI GGNNCC 1 cut(s) 712
BmrFI CCNGG 2 cut(s) 132, 715
BmsI GCATC 5 cut(s) 43, 325, 530, 847, 886
BpiI GAAGAC 1 cut(s) 78
BpmI CTGGAG 2 cut(s) 55, 69
Bpu10I CCTNAGC 2 cut(s) 119, 366
BpuEI CTTGAG 1 cut(s) 712
BsaI GGTCTC 2 cut(s) 45, 806
BsaJI CCNNGG 3 cut(s) 553, 713, 714
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 880
Bse1I ACTGG 3 cut(s) 38, 86, 292
BseBI CCWGG 2 cut(s) 132, 715
BseDI CCNNGG 3 cut(s) 553, 713, 714
BseGI GGATG 2 cut(s) 51, 901
BseLI CCNNNNNNNGG 2 cut(s) 329, 880
BseMII CTCAG 4 cut(s) 133, 147, 380, 686
BseNI ACTGG 3 cut(s) 38, 86, 292
BseRI GAGGAG 2 cut(s) 116, 812
BseXI GCAGC 2 cut(s) 102, 367
BsiHKCI CYCGRG 1 cut(s) 852
BslFI GGGAC 2 cut(s) 324, 371
BslI CCNNNNNNNGG 2 cut(s) 329, 880
BsmAI GTCTC 4 cut(s) 45, 179, 265, 806
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 2 cut(s) 324, 371
Bso31I GGTCTC 2 cut(s) 45, 806
BsoBI CYCGRG 1 cut(s) 852
Bsp143I GATC 2 cut(s) 319, 421
BspACI CCGC 5 cut(s) 56, 230, 355, 408, 884
BspCNI CTCAG 4 cut(s) 132, 148, 379, 685
BspLI GGNNCC 1 cut(s) 712
BspMAI CTGCAG 1 cut(s) 418
BspMI ACCTGC 1 cut(s) 421
BspPI GGATC 1 cut(s) 314
BspTNI GGTCTC 2 cut(s) 45, 806
BsrI ACTGG 3 cut(s) 38, 86, 292
BssECI CCNNGG 3 cut(s) 553, 713, 714
BssMI GATC 2 cut(s) 319, 421
BssT1I CCWWGG 1 cut(s) 553
Bst2UI CCWGG 2 cut(s) 132, 715
BstC8I GCNNGC 4 cut(s) 113, 408, 951, 955
BstDEI CTNAG 4 cut(s) 119, 156, 366, 672
BstENI CCTNNNNNAGG 2 cut(s) 327, 878
BstF5I GGATG 2 cut(s) 51, 901
BstHHI GCGC 1 cut(s) 291
BstKTI GATC 2 cut(s) 322, 424
BstMAI GTCTC 4 cut(s) 45, 179, 265, 806
BstMBI GATC 2 cut(s) 319, 421
BstMWI GCNNNNNNNGC 3 cut(s) 371, 380, 884
BstNI CCWGG 2 cut(s) 132, 715
BstNSI RCATGY 1 cut(s) 957
BstSCI CCNGG 2 cut(s) 130, 713
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 2 cut(s) 102, 367
BstV2I GAAGAC 1 cut(s) 78
BstX2I RGATCY 2 cut(s) 319, 421
BstYI RGATCY 2 cut(s) 319, 421
BsuI GTATCC 1 cut(s) 669
BtsCI GGATG 2 cut(s) 51, 901
BtsI GCAGTG 1 cut(s) 360
BtsIMutI CAGTG 2 cut(s) 285, 360
BveI ACCTGC 1 cut(s) 421
Cac8I GCNNGC 4 cut(s) 113, 408, 951, 955
CaiI CAGNNNCTG 1 cut(s) 284
CfoI GCGC 1 cut(s) 291
Cfr13I GGNCC 2 cut(s) 123, 710
CseI GACGC 1 cut(s) 161
Csp6I GTAC 3 cut(s) 31, 543, 836
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviAII CATG 1 cut(s) 954
CviQI GTAC 3 cut(s) 31, 543, 836
DdeI CTNAG 4 cut(s) 119, 156, 366, 672
DpnI GATC 2 cut(s) 321, 423
DpnII GATC 2 cut(s) 319, 421
DraI TTTAAA 1 cut(s) 568
DriI GACNNNNNGTC 1 cut(s) 730
Eam1105I GACNNNNNGTC 1 cut(s) 730
Eco130I CCWWGG 1 cut(s) 553
Eco31I GGTCTC 2 cut(s) 45, 806
Eco47I GGWCC 2 cut(s) 123, 710
Eco88I CYCGRG 1 cut(s) 852
EcoNI CCTNNNNNAGG 2 cut(s) 327, 878
EcoO109I RGGNCCY 1 cut(s) 123
EcoRI GAATTC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 130, 713
EcoT14I CCWWGG 1 cut(s) 553
ErhI CCWWGG 1 cut(s) 553
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 1 cut(s) 957
FaqI GGGAC 2 cut(s) 324, 371
FatI CATG 1 cut(s) 953
FauI CCCGC 2 cut(s) 348, 415
Fnu4HI GCNGC 4 cut(s) 57, 116, 381, 885
FokI GGATG 2 cut(s) 58, 908
Fsp4HI GCNGC 4 cut(s) 57, 116, 381, 885
FspBI CTAG 1 cut(s) 765
GlaI GCGC 1 cut(s) 290
GluI GCNGC 4 cut(s) 57, 116, 381, 885
GsuI CTGGAG 2 cut(s) 55, 69
HgaI GACGC 1 cut(s) 161
HhaI GCGC 1 cut(s) 291
Hin1II CATG 1 cut(s) 957
Hin6I GCGC 1 cut(s) 289
HinP1I GCGC 1 cut(s) 289
HincII GTYRAC 1 cut(s) 735
HindII GTYRAC 1 cut(s) 735
HinfI GANTC 8 cut(s) 5, 10, 26, 595, 670, 725, 761, 923
HphI GGTGA 2 cut(s) 541, 731
Hpy166II GTNNAC 3 cut(s) 91, 735, 836
Hpy188I TCNGA 7 cut(s) 15, 45, 67, 157, 394, 669, 675
Hpy188III TCNNGA 4 cut(s) 176, 256, 368, 854
Hpy8I GTNNAC 3 cut(s) 91, 735, 836
Hpy99I CGWCG 1 cut(s) 67
HpyAV CCTTC 2 cut(s) 199, 620
HpyCH4IV ACGT 2 cut(s) 62, 824
HpyCH4V TGCA 6 cut(s) 268, 338, 416, 521, 878, 899
HpyF10VI GCNNNNNNNGC 3 cut(s) 371, 380, 884
HpyF3I CTNAG 4 cut(s) 119, 156, 366, 672
HpySE526I ACGT 2 cut(s) 62, 824
Hsp92II CATG 1 cut(s) 957
HspAI GCGC 1 cut(s) 289
Kzo9I GATC 2 cut(s) 319, 421
LmnI GCTCC 2 cut(s) 88, 936
Lsp1109I GCAGC 2 cut(s) 102, 367
LweI GCATC 5 cut(s) 43, 325, 530, 847, 886
MaeI CTAG 1 cut(s) 765
MaeII ACGT 2 cut(s) 62, 824
MaeIII GTNAC 1 cut(s) 820
MalI GATC 2 cut(s) 321, 423
MboI GATC 2 cut(s) 319, 421
MboII GAAGA 3 cut(s) 83, 431, 596
MflI RGATCY 2 cut(s) 319, 421
MluCI AATT 5 cut(s) 95, 435, 688, 890, 982
MlyI GAGTC 5 cut(s) 19, 35, 604, 664, 719
MmeI TCCRAC 2 cut(s) 45, 789
MroXI GAANNNNTTC 1 cut(s) 651
MseI TTAA 6 cut(s) 198, 342, 426, 567, 908, 981
MspA1I CMGCKG 1 cut(s) 115
MspR9I CCNGG 2 cut(s) 132, 715
MvaI CCWGG 2 cut(s) 132, 715
MwoI GCNNNNNNNGC 3 cut(s) 371, 380, 884
NdeII GATC 2 cut(s) 319, 421
NlaIII CATG 1 cut(s) 957
NlaIV GGNNCC 1 cut(s) 712
NspI RCATGY 1 cut(s) 957
PaeI GCATGC 1 cut(s) 957
PaeR7I CTCGAG 1 cut(s) 852
PaqCI CACCTGC 1 cut(s) 421
PasI CCCWGGG 1 cut(s) 714
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 3 cut(s) 5, 761, 923
PflFI GACNNNGTC 1 cut(s) 77
PkrI GCNGC 4 cut(s) 58, 117, 382, 886
PleI GAGTC 5 cut(s) 18, 34, 603, 664, 719
PpsI GAGTC 5 cut(s) 18, 34, 603, 664, 719
PpuMI RGGWCCY 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 123
Psp6I CCWGG 2 cut(s) 130, 713
PspGI CCWGG 2 cut(s) 130, 713
PspN4I GGNNCC 1 cut(s) 712
PspPI GGNCC 2 cut(s) 123, 710
PspPPI RGGWCCY 1 cut(s) 123
PstI CTGCAG 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 284
PsuI RGATCY 2 cut(s) 319, 421
PsyI GACNNNGTC 1 cut(s) 77
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 3 cut(s) 32, 544, 837
RsaNI GTAC 3 cut(s) 31, 543, 836
SaqAI TTAA 6 cut(s) 198, 342, 426, 567, 908, 981
SatI GCNGC 4 cut(s) 57, 116, 381, 885
Sau3AI GATC 2 cut(s) 319, 421
Sau96I GGNCC 2 cut(s) 123, 710
ScaI AGTACT 1 cut(s) 544
SchI GAGTC 5 cut(s) 19, 35, 604, 664, 719
ScrFI CCNGG 2 cut(s) 132, 715
SfaNI GCATC 5 cut(s) 43, 325, 530, 847, 886
SfcI CTRYAG 1 cut(s) 414
Sfr274I CTCGAG 1 cut(s) 852
SinI GGWCC 2 cut(s) 123, 710
SlaI CTCGAG 1 cut(s) 852
SmlI CTYRAG 2 cut(s) 727, 852
SmoI CTYRAG 2 cut(s) 727, 852
SphI GCATGC 1 cut(s) 957
Sse9I AATT 5 cut(s) 95, 435, 688, 890, 982
SsiI CCGC 5 cut(s) 56, 230, 355, 408, 884
SspMI CTAG 1 cut(s) 765
StyD4I CCNGG 2 cut(s) 130, 713
StyI CCWWGG 1 cut(s) 553
TaiI ACGT 2 cut(s) 65, 827
TaqI TCGA 4 cut(s) 8, 51, 177, 853
TasI AATT 5 cut(s) 95, 435, 688, 890, 982
TatI WGTACW 1 cut(s) 542
TauI GCSGC 2 cut(s) 59, 887
TfiI GAWTC 3 cut(s) 5, 761, 923
Tru1I TTAA 6 cut(s) 198, 342, 426, 567, 908, 981
Tru9I TTAA 6 cut(s) 198, 342, 426, 567, 908, 981
TscAI CASTG 2 cut(s) 285, 367
TseI GCWGC 2 cut(s) 115, 380
TspDTI ATGAA 3 cut(s) 477, 596, 660
TspRI CASTG 2 cut(s) 285, 367
Tth111I GACNNNGTC 1 cut(s) 77
VpaK11BI GGWCC 2 cut(s) 123, 710
XagI CCTNNNNNAGG 2 cut(s) 327, 878
XapI RAATTY 2 cut(s) 435, 688
XceI RCATGY 1 cut(s) 957
XhoI CTCGAG 1 cut(s) 852
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 1 cut(s) 765
ZrmI AGTACT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.