RchiOBHm_Chr3g0450011

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016356
Physical Location & Seq
Reverse (-)
1659836 .. 1660571
736 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ41735

Sequence Viewer

Length: 705 bp
ATGGACTCTTCGGAGTTCTCTTTTGAGTCTTGTTCTTCGTTCAGCTCTCTGGGTTCTGATCTTTCCTGGGACTTGTTCAATTTTGATCACAGTTACAATCAAGTACAAGTACCCGCTCAGCTACCATTCAACGAAAACGACTCTGAAGAAATGGTTCTTTACGGAGTTCTTGCTCAGGTTGGTGCCGGAGACTCCTCCGACGCAACCAGTACTGCTCATCTTTCTAAAGATCATGAAGAGGTGAACTCCAACGCCGCCGTGGAATACGAGGAGCCTATGAAGGACGAGATAGCATATAGAGGGGTCCGCCGGAGGCCATGGGGGAAGTACGCGGCGGAGATAAGGGATTCAACCCGAAATGGGGTGCGGGTTTGGTTGGGAACTTTTGACACGGCGGAGGATGCAGCTCTAGCTTACGACCAAGCAGCATTTGCAATGCGGGGGACTCTGGCGGTACTTAATTTTCCGGCTGACGTTGTTTACCAGTCGCTCGTCGACATGAATTACGGGTTCGAAGAGGGCTCATCTCCGGTTTTGGCCTTGAAGAAAAGGCACTCCATGAAGAGGAAGTCATCGATGAACAAAAAGAAGAAGGACGTTCAGAAGTGTGATGATGATGTTGAGGAGTTGAGGTTGGAAAATGTGGTGGTTTTTGAGGATCTTGGTGCTGACTATTTGGAAGAGCTTCTCAGTATATCTTCTTAA

Protein Analysis

234

Amino Acids

26.18

Weight (kDa)

4.52

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 99 - 148 3.9e-15 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016356)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g02270
malus_domestica MD04G1228800.v1.1 MD12G1246000.v1.1
prunus_persica Prupe.6G348700_v2.0.a1
pyrus_communis pycom04g20280
rosa_chinensis RchiOBHm_Chr3g0450011
rosa_laevigata RLG00000025765
rosa_multiflora Rmu_co8520667.1_g000001
rosa_roxburghii Rroxscaffold_6G00427780
rosa_rugosa Rorug02G0624900
rosa_samantha Rh3AG023900 Rh3BG024500 Rh3CG023500 Rh3DG024500
rosa_wichuraiana Rw3G001900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 116
AccI GTMKAC 1 cut(s) 495
AccII CGCG 1 cut(s) 332
AciI CCGC 9 cut(s) 114, 255, 307, 332, 335, 367, 395, 439, 452
AclWI GGATC 1 cut(s) 666
AcuI CTGAAG 1 cut(s) 165
AfaI GTAC 5 cut(s) 105, 111, 211, 329, 456
AfiI CCNNNNNNNGG 3 cut(s) 360, 361, 564
AgsI TTSAA 4 cut(s) 79, 130, 351, 544
AjnI CCWGG 1 cut(s) 65
AluBI AGCT 5 cut(s) 45, 121, 407, 413, 685
AluI AGCT 5 cut(s) 45, 121, 407, 413, 685
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 1 cut(s) 666
AoxI GGCC 2 cut(s) 314, 537
ApeKI GCWGC 2 cut(s) 404, 425
Asp700I GAANNNNTTC 2 cut(s) 153, 684
AspS9I GGNCC 1 cut(s) 304
AsuHPI GGTGA 1 cut(s) 253
AsuII TTCGAA 1 cut(s) 513
AvaII GGWCC 1 cut(s) 304
BanI GGYRCC 1 cut(s) 182
BanII GRGCYC 1 cut(s) 524
BbvI GCAGC 2 cut(s) 416, 437
BceAI ACGGC 2 cut(s) 242, 408
BciT130I CCWGG 1 cut(s) 67
BclI TGATCA 1 cut(s) 85
BcoDI GTCTC 1 cut(s) 183
BfaI CTAG 1 cut(s) 410
BisI GCNGC 4 cut(s) 255, 333, 405, 426
BlpI GCTNAGC 1 cut(s) 117
BlsI GCNGC 4 cut(s) 256, 334, 406, 427
BmcAI AGTACT 1 cut(s) 211
Bme1390I CCNGG 1 cut(s) 67
Bme18I GGWCC 1 cut(s) 304
BmgT120I GGNCC 1 cut(s) 304
BmiI GGNNCC 3 cut(s) 184, 273, 305
BmrFI CCNGG 1 cut(s) 67
BmsI GCATC 1 cut(s) 391
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu10I CCTNAGC 1 cut(s) 174
Bpu1102I GCTNAGC 1 cut(s) 117
Bpu14I TTCGAA 1 cut(s) 513
Bsa29I ATCGAT 1 cut(s) 575
BsaJI CCNNGG 3 cut(s) 66, 258, 317
BsaWI WCCGGW 1 cut(s) 529
BsaXI ACNNNNNCTCC 2 cut(s) 617, 647
Bsc4I CCNNNNNNNGG 3 cut(s) 360, 361, 564
Bse1I ACTGG 2 cut(s) 207, 484
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 67
BseCI ATCGAT 1 cut(s) 575
BseDI CCNNGG 3 cut(s) 66, 258, 317
BseGI GGATG 1 cut(s) 406
BseLI CCNNNNNNNGG 3 cut(s) 360, 361, 564
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 3 cut(s) 131, 188, 703
BseNI ACTGG 2 cut(s) 207, 484
BseRI GAGGAG 3 cut(s) 184, 284, 638
BseXI GCAGC 2 cut(s) 416, 437
Bsh1236I CGCG 1 cut(s) 332
BshFI GGCC 2 cut(s) 316, 539
BshNI GGYRCC 1 cut(s) 182
BshVI ATCGAT 1 cut(s) 575
BsiSI CCGG 4 cut(s) 186, 310, 467, 530
BslFI GGGAC 2 cut(s) 83, 457
BslI CCNNNNNNNGG 3 cut(s) 360, 361, 564
BsmAI GTCTC 1 cut(s) 183
BsmFI GGGAC 2 cut(s) 83, 457
BsnI GGCC 2 cut(s) 316, 539
Bsp119I TTCGAA 1 cut(s) 513
Bsp1286I GDGCHC 1 cut(s) 524
Bsp143I GATC 4 cut(s) 58, 85, 229, 658
Bsp1720I GCTNAGC 1 cut(s) 117
Bsp19I CCATGG 1 cut(s) 317
BspACI CCGC 9 cut(s) 114, 255, 307, 332, 335, 367, 395, 439, 452
BspANI GGCC 2 cut(s) 316, 539
BspCNI CTCAG 3 cut(s) 130, 187, 702
BspDI ATCGAT 1 cut(s) 575
BspFNI CGCG 1 cut(s) 332
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 3 cut(s) 184, 273, 305
BspPI GGATC 1 cut(s) 666
BspQI GCTCTTC 1 cut(s) 675
BspT104I TTCGAA 1 cut(s) 513
BspT107I GGYRCC 1 cut(s) 182
BsrBI CCGCTC 1 cut(s) 116
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 2 cut(s) 207, 484
BssECI CCNNGG 3 cut(s) 66, 258, 317
BssMI GATC 4 cut(s) 58, 85, 229, 658
BssT1I CCWWGG 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 67
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 5 cut(s) 13, 231, 510, 557, 675
BstAPI GCANNNNNTGC 1 cut(s) 431
BstBI TTCGAA 1 cut(s) 513
BstDEI CTNAG 3 cut(s) 117, 174, 689
BstDSI CCRYGG 2 cut(s) 258, 317
BstF5I GGATG 1 cut(s) 406
BstFNI CGCG 1 cut(s) 332
BstKTI GATC 4 cut(s) 61, 88, 232, 661
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 4 cut(s) 58, 85, 229, 658
BstMWI GCNNNNNNNGC 3 cut(s) 401, 410, 431
BstNI CCWGG 1 cut(s) 67
BstSCI CCNGG 1 cut(s) 65
BstUI CGCG 1 cut(s) 332
BstV1I GCAGC 2 cut(s) 416, 437
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
Bsu15I ATCGAT 1 cut(s) 575
BsuRI GGCC 2 cut(s) 316, 539
BsuTUI ATCGAT 1 cut(s) 575
BtgI CCRYGG 2 cut(s) 258, 317
BtsCI GGATG 1 cut(s) 406
CciI TCATGA 1 cut(s) 232
Cfr13I GGNCC 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 575
CseI GACGC 1 cut(s) 209
Csp6I GTAC 5 cut(s) 104, 110, 210, 328, 455
CviAII CATG 4 cut(s) 233, 318, 499, 559
CviQI GTAC 5 cut(s) 104, 110, 210, 328, 455
DdeI CTNAG 3 cut(s) 117, 174, 689
DpnI GATC 4 cut(s) 60, 87, 231, 660
DpnII GATC 4 cut(s) 58, 85, 229, 658
Eam1104I CTCTTC 5 cut(s) 13, 231, 510, 557, 675
EarI CTCTTC 5 cut(s) 13, 231, 510, 557, 675
EciI GGCGGA 3 cut(s) 296, 350, 410
Eco130I CCWWGG 1 cut(s) 317
Eco24I GRGCYC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 304
Eco57I CTGAAG 1 cut(s) 165
EcoRII CCWGG 1 cut(s) 65
EcoT14I CCWWGG 1 cut(s) 317
EcoT38I GRGCYC 1 cut(s) 524
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 4 cut(s) 236, 321, 502, 562
FaiI YATR 8 cut(s) 234, 278, 295, 297, 319, 500, 560, 695
FaqI GGGAC 2 cut(s) 83, 457
FatI CATG 4 cut(s) 232, 317, 498, 558
FauI CCCGC 3 cut(s) 121, 360, 432
FbaI TGATCA 1 cut(s) 85
FblI GTMKAC 1 cut(s) 495
Fnu4HI GCNGC 4 cut(s) 255, 333, 405, 426
FokI GGATG 1 cut(s) 413
FriOI GRGCYC 1 cut(s) 524
Fsp4HI GCNGC 4 cut(s) 255, 333, 405, 426
FspBI CTAG 1 cut(s) 410
GluI GCNGC 4 cut(s) 255, 333, 405, 426
HaeIII GGCC 2 cut(s) 316, 539
HapII CCGG 4 cut(s) 186, 310, 467, 530
HgaI GACGC 1 cut(s) 209
Hin1II CATG 4 cut(s) 236, 321, 502, 562
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HinfI GANTC 6 cut(s) 5, 26, 140, 191, 347, 445
HpaII CCGG 4 cut(s) 186, 310, 467, 530
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 3 cut(s) 244, 481, 496
Hpy188I TCNGA 5 cut(s) 13, 58, 145, 199, 603
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 3 cut(s) 244, 481, 496
Hpy99I CGWCG 2 cut(s) 203, 497
HpyAV CCTTC 2 cut(s) 274, 586
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 2 cut(s) 474, 597
HpyCH4V TGCA 2 cut(s) 404, 434
HpyF10VI GCNNNNNNNGC 3 cut(s) 401, 410, 431
HpyF3I CTNAG 3 cut(s) 117, 174, 689
HpySE526I ACGT 2 cut(s) 474, 597
Hsp92II CATG 4 cut(s) 236, 321, 502, 562
Ksp22I TGATCA 1 cut(s) 85
Kzo9I GATC 4 cut(s) 58, 85, 229, 658
LguI GCTCTTC 1 cut(s) 675
LmnI GCTCC 1 cut(s) 271
Lsp1109I GCAGC 2 cut(s) 416, 437
LweI GCATC 1 cut(s) 391
MaeI CTAG 1 cut(s) 410
MaeII ACGT 2 cut(s) 474, 597
MaeIII GTNAC 1 cut(s) 92
MalI GATC 4 cut(s) 60, 87, 231, 660
MbiI CCGCTC 1 cut(s) 116
MboI GATC 4 cut(s) 58, 85, 229, 658
MboII GAAGA 9 cut(s) 27, 158, 248, 527, 556, 574, 601, 690, 692
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 524
MluCI AATT 3 cut(s) 79, 460, 502
MlyI GAGTC 4 cut(s) 35, 134, 185, 439
MmeI TCCRAC 3 cut(s) 222, 273, 615
MroXI GAANNNNTTC 2 cut(s) 153, 684
MseI TTAA 2 cut(s) 459, 703
MspI CCGG 4 cut(s) 186, 310, 467, 530
MspR9I CCNGG 1 cut(s) 67
MvaI CCWGG 1 cut(s) 67
MvnI CGCG 1 cut(s) 332
MwoI GCNNNNNNNGC 3 cut(s) 401, 410, 431
NcoI CCATGG 1 cut(s) 317
NdeII GATC 4 cut(s) 58, 85, 229, 658
NlaIII CATG 4 cut(s) 236, 321, 502, 562
NlaIV GGNNCC 3 cut(s) 184, 273, 305
NspV TTCGAA 1 cut(s) 513
PagI TCATGA 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 675
PdmI GAANNNNTTC 2 cut(s) 153, 684
PfeI GAWTC 1 cut(s) 347
PkrI GCNGC 4 cut(s) 256, 334, 406, 427
PleI GAGTC 4 cut(s) 34, 134, 185, 439
PpsI GAGTC 4 cut(s) 34, 134, 185, 439
Psp6I CCWGG 1 cut(s) 65
PspGI CCWGG 1 cut(s) 65
PspN4I GGNNCC 3 cut(s) 184, 273, 305
PspPI GGNCC 1 cut(s) 304
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 5 cut(s) 105, 111, 211, 329, 456
RsaNI GTAC 5 cut(s) 104, 110, 210, 328, 455
SalI GTCGAC 1 cut(s) 494
SapI GCTCTTC 1 cut(s) 675
SaqAI TTAA 2 cut(s) 459, 703
SatI GCNGC 4 cut(s) 255, 333, 405, 426
Sau3AI GATC 4 cut(s) 58, 85, 229, 658
Sau96I GGNCC 1 cut(s) 304
ScaI AGTACT 1 cut(s) 211
SchI GAGTC 4 cut(s) 35, 134, 185, 439
ScrFI CCNGG 1 cut(s) 67
SduI GDGCHC 1 cut(s) 524
SfaNI GCATC 1 cut(s) 391
SfuI TTCGAA 1 cut(s) 513
SinI GGWCC 1 cut(s) 304
Sse9I AATT 3 cut(s) 79, 460, 502
SsiI CCGC 9 cut(s) 114, 255, 307, 332, 335, 367, 395, 439, 452
SspMI CTAG 1 cut(s) 410
StyD4I CCNGG 1 cut(s) 65
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 2 cut(s) 477, 600
TaqI TCGA 3 cut(s) 495, 513, 575
TasI AATT 3 cut(s) 79, 460, 502
TatI WGTACW 2 cut(s) 103, 209
TauI GCSGC 2 cut(s) 257, 335
TfiI GAWTC 1 cut(s) 347
Tru1I TTAA 2 cut(s) 459, 703
Tru9I TTAA 2 cut(s) 459, 703
TseI GCWGC 2 cut(s) 404, 425
TspDTI ATGAA 5 cut(s) 249, 293, 515, 575, 593
TspGWI ACGGA 1 cut(s) 177
VpaK11BI GGWCC 1 cut(s) 304
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XmiI GTMKAC 1 cut(s) 495
XmnI GAANNNNTTC 2 cut(s) 153, 684
XspI CTAG 1 cut(s) 410
ZrmI AGTACT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.