RchiOBHm_Chr3g0453101

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016867
Physical Location & Seq
Forward (+)
3629434 .. 3632716
3283 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42021

Sequence Viewer

Length: 2232 bp
ATGGAAGAGACAGCTCCGGTGACTGCGAAAGACTCGGTCTTGAAGCGGCAGAGAAGCTTCGGCAAAGCGTACAAGACGTTCCAAACGCAGGCGACTTCGCTCGTCGGGTTGACCCGGCAGTTCAAGGAGCTCCAGGCGCGGTGGGGGTCCACGGAGGACGAGCTCCGGACGCGGTTCAGGGAAGTCCGGGACCGGGAAAGGGAGGTCGGCGCGAGAGAGAAGCAGTTGGAGGAGAAAGAGGCGAAGTGGAAGGTCGAGATGGAAGCGCAGAAGAAAGAGGTGGAATCGAGTAAGCACCAGTTGCTTTTCCTGAGGCCATTGATCAGTAAACGTGTTCAAGAGCTTGATGGTTTGAAAGTGATGGTGAGTGAGAAGAAGAAAGAGCGTGATTTGATCGAAAAAAGGGGTTTAGAGTGGACTGAGAGATTGAATTCTGTTGAGAAATGTGTGGAGGAGAAGAAGAAAGAGGTCGAGGCGAAGGAGGAGGAAGTGAGAGGGGTGGAAGGGAGGTTGAGGATTTATGGGGAGGAGATTGAGATGAAGGAGAGGGAGTTGAGTGGGATTTTGGGGTCAATTGAGGAGTGCAGGAGAGAATGTGGTTTGAAAGAAGAGGAAATTAAGGCGAAGCGAAGGTGTGTTGATGAATGGGAGAAGGAGATGGAATTGAAGAGAGAGGAACTTGTTGATCAGCTTGATGCTATGAAAATACTAGTTAGAGAGAAGGGGAAGGAGTGTGAGTTGATTGAAAATCGGGTTTTGGAGTCATCAACGAGATCTTATTATTTTGGGAAATGTGTAGAAGAGAGGAGGAAAGAGTTTGAGGCTAAGGAAAAGGAACTGAAAGAGGTTCAGAGGTGTATTGAGTTGAAGGAGAGGCAATTAGATGGGATTTTGAAGTCGATTCAGGAGCACACGAAAGAATGTGATTTGAAAGGAAAGCAAGTTAAGGCATTGGAGTGGTCCATTGAGAAATGTGAAAAAGAGATGGAATTGAAAAGGGAGGAGTGGTCTCGTAAGCTTGAGTTGAAGGAGATGGAACTAGAAAGGTTGTTTGACAAGTTTGAGTTGAGAGGGCAACAACTTGAGCCGATAGTTGAAGAGCTTCGTGTTATTGATAAGAGGGTGGATGGATGCTTAAATGAGGGTCTGTGTAAAGAGAGGTACTTGCAGACATGTGAAAAATTGATACAAGAACGCGAAAGGTGTTTGGATTCAGTGTCCCATGAACTTAAGATGAAAGAGAGGCACATTGATGAGGAGGCCAAAGAGCTTGAATTGAAGCAAATAAAATTTTATTCTCAACTGAAGACTCAGCAGTTGGAAACAGAAAAGCATTTTGCTTCACTTAAAAAATCGTTAGAACAACAAGAACAGTCTTTGTATGCACTCCGAAAGTCGATAGAAGAGGTCAAAAAGCTTTTGGAGAGGCAACTTGAACAGCAGGGTGGAGTGATTGAATTGAAACAAAAACCATTTGATTCTCAGCTATTGGAAAGAGAAGAGCAACTTGGTTCAATTGAAAAGTCAATAGAAGAATGTGATAAGCAGTTCAACGGAGTTCAAAAGTTGGTTCAAGAGCAGAAAAAGCATTTGGATTCCCTTTCCCATACACTCCATATAAGAGAGACGCAAGCTGAAAAGCAGGACAAACAGCTTGAATTGAAGTTTAGACATTCTGATTCTGAAGTGAATACTAAGCAGTTGGAACTCACCCCTGCCGCTGATGATGATATGATTAATCCTCACTCTATGAGTGATCAACCCAACATCAATAGGGATGGTAGAAGCTTCGAGGTGATCCCTGGTATTATTCGGAACCTTATCGAAAGAAAGCAGCTGCTTAAGGCTGTTACATATATCTGCACCTTCAAGCAAATTGACAAGTTTCCCCCAGTACCACTCTTGAAAGAATTTGTGGAGGATGCAAAGAAGTCTTGCACTGAAATTTTCAGTAGAGAGATATCACATGATGTAAAGGAGAAGGTTGTAGATGACCTAATAGCTGATCTAAGAGCTCTGATTCAGTGCATTGAATATCACAGCCTCGAGTCACAGTACCCACCAACTGACATCATGATGGAAATTCAAGGGCTTGGGGAACTAAAGGAGAATCCGAAGCTGCTATCTCTTCCGTCCGATGTTGAAAAACAAGAGCAGAGAAAAGGAAGGAAACGAAGTTCCAGCAGTGGGGGGAGCACATCTCACACATATCAGCTGCGTAAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

743

Amino Acids

87.77

Weight (kDa)

6.0

Isoelectric Point (pI)

58.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 599 - 706 4.4e-22 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016867)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04511 FvH4_6g04590 FvH4_6g04630
rosa_chinensis RchiOBHm_Chr3g0453101
rosa_laevigata RLG00000025537
rosa_multiflora Rmu_sc0009015.1_g000009
rosa_roxburghii Rroxscaffold_6G00430170
rosa_rugosa Rorug02G0646500 Rorug02G0646600
rosa_samantha Rh3AG049100 Rh3BG050300 Rh3CG050400 Rh3DG051100
rosa_wichuraiana Rw3G003760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 139, 172, 212, 1197
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 4 cut(s) 46, 139, 172, 1719
AclWI GGATC 1 cut(s) 1792
AcsI RAATTY 5 cut(s) 430, 1289, 1910, 1944, 2082
AcuI CTGAAG 2 cut(s) 1325, 1704
AfaI GTAC 4 cut(s) 71, 1163, 1896, 2057
AfiI CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2187
AflII CTTAAG 2 cut(s) 1229, 1841
AflIII ACRYGT 2 cut(s) 331, 1172
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 2 cut(s) 132, 1801
AjuI GAANNNNNNNTTGG 4 cut(s) 1491, 1523, 1574, 1606
AloI GAACNNNNNNTCC 2 cut(s) 2161, 2193
Alw21I GWGCWC 5 cut(s) 132, 165, 912, 2017, 2198
Alw26I GTCTC 3 cut(s) 2, 1014, 1619
AlwI GGATC 1 cut(s) 1792
Ama87I CYCGRG 1 cut(s) 2045
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 2 cut(s) 314, 1260
ApeKI GCWGC 4 cut(s) 1834, 1837, 2119, 2215
ApoI RAATTY 5 cut(s) 430, 1289, 1910, 1944, 2082
AseI ATTAAT 1 cut(s) 1737
Asp700I GAANNNNTTC 1 cut(s) 1101
AspLEI GCGC 3 cut(s) 139, 212, 268
AspS9I GGNCC 3 cut(s) 147, 190, 960
AsuC2I CCSGG 3 cut(s) 115, 188, 194
AsuHPI GGTGA 4 cut(s) 31, 376, 1702, 1807
AvaI CYCGRG 1 cut(s) 2045
AvaII GGWCC 3 cut(s) 147, 190, 960
AxyI CCTNAGG 1 cut(s) 311
BanII GRGCYC 3 cut(s) 132, 165, 2017
BbsI GAAGAC 1 cut(s) 1313
Bbv12I GWGCWC 5 cut(s) 132, 165, 912, 2017, 2198
BbvI GCAGC 4 cut(s) 1824, 1846, 2106, 2202
BciT130I CCWGG 2 cut(s) 134, 1803
BclI TGATCA 3 cut(s) 321, 685, 1756
BcnI CCSGG 3 cut(s) 115, 188, 194
BcoDI GTCTC 3 cut(s) 2, 1014, 1619
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 2 cut(s) 710, 1040
BfrI CTTAAG 2 cut(s) 1229, 1841
BglII AGATCT 1 cut(s) 773
BisI GCNGC 6 cut(s) 47, 1719, 1835, 1838, 2120, 2216
BlsI GCNGC 6 cut(s) 48, 1720, 1836, 1839, 2121, 2217
Bme1390I CCNGG 5 cut(s) 115, 134, 188, 194, 1803
Bme18I GGWCC 3 cut(s) 147, 190, 960
BmeT110I CYCGRG 1 cut(s) 2045
BmgT120I GGNCC 3 cut(s) 147, 190, 960
BmiI GGNNCC 3 cut(s) 148, 191, 1817
BmrFI CCNGG 5 cut(s) 115, 134, 188, 194, 1803
BmrI ACTGGG 1 cut(s) 1886
BmsI GCATC 3 cut(s) 685, 1121, 1912
BmuI ACTGGG 1 cut(s) 1886
BpiI GAAGAC 1 cut(s) 1313
BplI GAGNNNNNCTC 2 cut(s) 2185, 2217
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 825
BpuEI CTTGAG 2 cut(s) 1040, 1103
BpuMI CCSGG 3 cut(s) 115, 188, 194
BsaI GGTCTC 1 cut(s) 1014
BsaJI CCNNGG 2 cut(s) 150, 1801
BsaWI WCCGGW 2 cut(s) 16, 165
BsaXI ACNNNNNCTCC 8 cut(s) 536, 566, 580, 610, 722, 752, 992, 1022
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2187
Bse1I ACTGG 2 cut(s) 298, 1892
Bse21I CCTNAGG 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 2 cut(s) 134, 1803
BseDI CCNNGG 2 cut(s) 150, 1801
BseGI GGATG 4 cut(s) 1132, 1136, 1783, 1927
BseLI CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2187
BseMII CTCAG 4 cut(s) 302, 411, 1325, 1496
BseNI ACTGG 2 cut(s) 298, 1892
BseRI GAGGAG 8 cut(s) 245, 467, 497, 542, 593, 820, 1016, 1271
BseXI GCAGC 4 cut(s) 1824, 1846, 2106, 2202
BsgI GTGCAG 2 cut(s) 604, 1846
Bsh1236I CGCG 4 cut(s) 139, 172, 212, 1197
BshFI GGCC 2 cut(s) 316, 1262
BsiHKAI GWGCWC 5 cut(s) 132, 165, 912, 2017, 2198
BsiHKCI CYCGRG 1 cut(s) 2045
BsiSI CCGG 5 cut(s) 17, 115, 166, 187, 193
BslFI GGGAC 2 cut(s) 203, 1204
BslI CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2187
BsmAI GTCTC 3 cut(s) 2, 1014, 1619
BsmBI CGTCTC 1 cut(s) 1619
BsmFI GGGAC 2 cut(s) 203, 1204
BsnI GGCC 2 cut(s) 316, 1262
Bso31I GGTCTC 1 cut(s) 1014
BsoBI CYCGRG 1 cut(s) 2045
Bsp1286I GDGCHC 5 cut(s) 132, 165, 912, 2017, 2198
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
BspACI CCGC 4 cut(s) 46, 139, 172, 1719
BspANI GGCC 2 cut(s) 316, 1262
BspCNI CTCAG 4 cut(s) 303, 412, 1324, 1495
BspEI TCCGGA 1 cut(s) 165
BspFNI CGCG 4 cut(s) 139, 172, 212, 1197
BspHI TCATGA 1 cut(s) 2073
BspLI GGNNCC 3 cut(s) 148, 191, 1817
BspPI GGATC 1 cut(s) 1792
BspQI GCTCTTC 2 cut(s) 1092, 1494
BspTI CTTAAG 2 cut(s) 1229, 1841
BspTNI GGTCTC 1 cut(s) 1014
BsrI ACTGG 2 cut(s) 298, 1892
BssECI CCNNGG 2 cut(s) 150, 1801
BssMI GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
Bst2UI CCWGG 2 cut(s) 134, 1803
Bst4CI ACNGT 2 cut(s) 1374, 2055
Bst6I CTCTTC 7 cut(s) 603, 662, 795, 1092, 1398, 1494, 2133
BstAFI CTTAAG 2 cut(s) 1229, 1841
BstAPI GCANNNNNTGC 1 cut(s) 301
BstC8I GCNNGC 2 cut(s) 90, 1632
BstDEI CTNAG 7 cut(s) 311, 420, 825, 1311, 1482, 1695, 2009
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 4 cut(s) 1132, 1136, 1783, 1927
BstFNI CGCG 4 cut(s) 139, 172, 212, 1197
BstHHI GCGC 3 cut(s) 139, 212, 268
BstKTI GATC 7 cut(s) 324, 396, 688, 776, 1759, 1800, 2008
BstMAI GTCTC 3 cut(s) 2, 1014, 1619
BstMBI GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
BstMWI GCNNNNNNNGC 4 cut(s) 136, 169, 301, 1585
BstNI CCWGG 2 cut(s) 134, 1803
BstNSI RCATGY 1 cut(s) 1176
BstSCI CCNGG 5 cut(s) 113, 132, 186, 192, 1801
BstUI CGCG 4 cut(s) 139, 172, 212, 1197
BstV1I GCAGC 4 cut(s) 1824, 1846, 2106, 2202
BstV2I GAAGAC 1 cut(s) 1313
BstX2I RGATCY 1 cut(s) 773
BstYI RGATCY 1 cut(s) 773
Bsu36I CCTNAGG 1 cut(s) 311
BsuRI GGCC 2 cut(s) 316, 1262
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 4 cut(s) 1132, 1136, 1783, 1927
BtsI GCAGTG 1 cut(s) 2191
BtsIMutI CAGTG 4 cut(s) 1221, 1938, 2030, 2191
Cac8I GCNNGC 2 cut(s) 90, 1632
CciI TCATGA 1 cut(s) 2073
CfoI GCGC 3 cut(s) 139, 212, 268
Cfr13I GGNCC 3 cut(s) 147, 190, 960
CseI GACGC 2 cut(s) 178, 1636
Csp6I GTAC 4 cut(s) 70, 1162, 1895, 2056
CviAII CATG 4 cut(s) 1173, 1223, 1967, 2074
CviQI GTAC 4 cut(s) 70, 1162, 1895, 2056
DdeI CTNAG 7 cut(s) 311, 420, 825, 1311, 1482, 1695, 2009
DpnI GATC 7 cut(s) 323, 395, 687, 775, 1758, 1799, 2007
DpnII GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
Eam1104I CTCTTC 7 cut(s) 603, 662, 795, 1092, 1398, 1494, 2133
EarI CTCTTC 7 cut(s) 603, 662, 795, 1092, 1398, 1494, 2133
Ecl136II GAGCTC 3 cut(s) 130, 163, 2015
Eco24I GRGCYC 3 cut(s) 132, 165, 2017
Eco31I GGTCTC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 1962
Eco47I GGWCC 3 cut(s) 147, 190, 960
Eco53kI GAGCTC 3 cut(s) 130, 163, 2015
Eco57I CTGAAG 2 cut(s) 1325, 1704
Eco81I CCTNAGG 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 2045
EcoICRI GAGCTC 3 cut(s) 130, 163, 2015
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 132, 1801
EcoRV GATATC 1 cut(s) 1962
EcoT38I GRGCYC 3 cut(s) 132, 165, 2017
Esp3I CGTCTC 1 cut(s) 1619
FaeI CATG 4 cut(s) 1176, 1226, 1970, 2077
FalI AAGNNNNNCTT 2 cut(s) 1491, 1523
FaqI GGGAC 2 cut(s) 203, 1204
FatI CATG 4 cut(s) 1172, 1222, 1966, 2073
FbaI TGATCA 3 cut(s) 321, 685, 1756
Fnu4HI GCNGC 6 cut(s) 47, 1719, 1835, 1838, 2120, 2216
FokI GGATG 4 cut(s) 1139, 1143, 1790, 1934
FriOI GRGCYC 3 cut(s) 132, 165, 2017
Fsp4HI GCNGC 6 cut(s) 47, 1719, 1835, 1838, 2120, 2216
FspBI CTAG 2 cut(s) 710, 1040
GlaI GCGC 3 cut(s) 138, 211, 267
GluI GCNGC 6 cut(s) 47, 1719, 1835, 1838, 2120, 2216
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 2 cut(s) 316, 1262
HapII CCGG 5 cut(s) 17, 115, 166, 187, 193
HgaI GACGC 2 cut(s) 178, 1636
HhaI GCGC 3 cut(s) 139, 212, 268
Hin1II CATG 4 cut(s) 1176, 1226, 1970, 2077
Hin6I GCGC 3 cut(s) 137, 210, 266
HinP1I GCGC 3 cut(s) 137, 210, 266
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 4 cut(s) 55, 1016, 1415, 1786
HpaII CCGG 5 cut(s) 17, 115, 166, 187, 193
HphI GGTGA 4 cut(s) 31, 376, 1702, 1807
Hpy166II GTNNAC 4 cut(s) 111, 150, 329, 417
Hpy188I TCNGA 8 cut(s) 852, 1391, 1678, 1684, 1815, 2019, 2115, 2137
Hpy188III TCNNGA 9 cut(s) 40, 166, 256, 310, 338, 905, 1574, 1903, 2074
Hpy8I GTNNAC 4 cut(s) 111, 150, 329, 417
Hpy99I CGWCG 1 cut(s) 107
HpyCH4III ACNGT 2 cut(s) 1374, 2055
HpyCH4IV ACGT 2 cut(s) 77, 331
HpyCH4V TGCA 7 cut(s) 585, 1168, 1385, 1863, 1925, 1938, 2028
HpyF10VI GCNNNNNNNGC 4 cut(s) 136, 169, 301, 1585
HpyF3I CTNAG 7 cut(s) 311, 420, 825, 1311, 1482, 1695, 2009
HpySE526I ACGT 2 cut(s) 77, 331
Hsp92II CATG 4 cut(s) 1176, 1226, 1970, 2077
HspAI GCGC 3 cut(s) 137, 210, 266
Kpn2I TCCGGA 1 cut(s) 165
Ksp22I TGATCA 3 cut(s) 321, 685, 1756
Kzo9I GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
LguI GCTCTTC 2 cut(s) 1092, 1494
LmnI GCTCC 6 cut(s) 19, 127, 135, 168, 907, 2193
Lsp1109I GCAGC 4 cut(s) 1824, 1846, 2106, 2202
LweI GCATC 3 cut(s) 685, 1121, 1912
MaeI CTAG 2 cut(s) 710, 1040
MaeII ACGT 2 cut(s) 77, 331
MaeIII GTNAC 3 cut(s) 19, 1849, 2049
MalI GATC 7 cut(s) 323, 395, 687, 775, 1758, 1799, 2007
MboI GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
MfeI CAATTG 2 cut(s) 573, 1515
MflI RGATCY 1 cut(s) 773
MhlI GDGCHC 5 cut(s) 132, 165, 912, 2017, 2198
MlyI GAGTC 4 cut(s) 26, 770, 1303, 2057
MmeI TCCRAC 3 cut(s) 207, 1299, 1683
MroI TCCGGA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 1101
MseI TTAA 7 cut(s) 618, 945, 1136, 1230, 1347, 1737, 1842
MslI CAYNNNNRTG 3 cut(s) 955, 1251, 2075
MspA1I CMGCKG 3 cut(s) 1721, 1837, 2215
MspCI CTTAAG 2 cut(s) 1229, 1841
MspI CCGG 5 cut(s) 17, 115, 166, 187, 193
MspR9I CCNGG 5 cut(s) 115, 134, 188, 194, 1803
MunI CAATTG 2 cut(s) 573, 1515
MvaI CCWGG 2 cut(s) 134, 1803
MvnI CGCG 4 cut(s) 139, 172, 212, 1197
MwoI GCNNNNNNNGC 4 cut(s) 136, 169, 301, 1585
NciI CCSGG 3 cut(s) 115, 188, 194
NdeII GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
NlaIII CATG 4 cut(s) 1176, 1226, 1970, 2077
NlaIV GGNNCC 3 cut(s) 148, 191, 1817
NmuCI GTSAC 2 cut(s) 19, 2049
NspI RCATGY 1 cut(s) 1176
PaeR7I CTCGAG 1 cut(s) 2045
PagI TCATGA 1 cut(s) 2073
PciI ACATGT 1 cut(s) 1172
PciSI GCTCTTC 2 cut(s) 1092, 1494
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 8 cut(s) 284, 901, 1211, 1478, 1595, 1679, 2020, 2110
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 6 cut(s) 48, 1720, 1836, 1839, 2121, 2217
PleI GAGTC 4 cut(s) 26, 769, 1303, 2056
PpsI GAGTC 4 cut(s) 26, 769, 1303, 2056
PscI ACATGT 1 cut(s) 1172
PshBI ATTAAT 1 cut(s) 1737
Psp124BI GAGCTC 3 cut(s) 132, 165, 2017
Psp6I CCWGG 2 cut(s) 132, 1801
PspGI CCWGG 2 cut(s) 132, 1801
PspN4I GGNNCC 3 cut(s) 148, 191, 1817
PspPI GGNCC 3 cut(s) 147, 190, 960
PspXI VCTCGAGB 1 cut(s) 2045
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PsuI RGATCY 1 cut(s) 773
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 2 cut(s) 1837, 2215
RsaI GTAC 4 cut(s) 71, 1163, 1896, 2057
RsaNI GTAC 4 cut(s) 70, 1162, 1895, 2056
RseI CAYNNNNRTG 3 cut(s) 955, 1251, 2075
SacI GAGCTC 3 cut(s) 132, 165, 2017
SapI GCTCTTC 2 cut(s) 1092, 1494
SaqAI TTAA 7 cut(s) 618, 945, 1136, 1230, 1347, 1737, 1842
SatI GCNGC 6 cut(s) 47, 1719, 1835, 1838, 2120, 2216
Sau3AI GATC 7 cut(s) 321, 393, 685, 773, 1756, 1797, 2005
Sau96I GGNCC 3 cut(s) 147, 190, 960
SchI GAGTC 4 cut(s) 26, 770, 1303, 2057
ScrFI CCNGG 5 cut(s) 115, 134, 188, 194, 1803
SduI GDGCHC 5 cut(s) 132, 165, 912, 2017, 2198
SfaNI GCATC 3 cut(s) 685, 1121, 1912
Sfr274I CTCGAG 1 cut(s) 2045
SinI GGWCC 3 cut(s) 147, 190, 960
SlaI CTCGAG 1 cut(s) 2045
SmiMI CAYNNNNRTG 3 cut(s) 955, 1251, 2075
SmlI CTYRAG 5 cut(s) 1019, 1082, 1229, 1841, 2045
SmoI CTYRAG 5 cut(s) 1019, 1082, 1229, 1841, 2045
SpeI ACTAGT 1 cut(s) 709
SsiI CCGC 4 cut(s) 46, 139, 172, 1719
SspMI CTAG 2 cut(s) 710, 1040
SstI GAGCTC 3 cut(s) 132, 165, 2017
StyD4I CCNGG 5 cut(s) 113, 132, 186, 192, 1801
TaaI ACNGT 2 cut(s) 1374, 2055
TaiI ACGT 2 cut(s) 80, 334
TaqI TCGA 9 cut(s) 255, 287, 396, 471, 899, 1397, 1791, 1824, 2046
TaqII GACCGA 1 cut(s) 25
TauI GCSGC 2 cut(s) 49, 1721
TfiI GAWTC 8 cut(s) 284, 901, 1211, 1478, 1595, 1679, 2020, 2110
Tru1I TTAA 7 cut(s) 618, 945, 1136, 1230, 1347, 1737, 1842
Tru9I TTAA 7 cut(s) 618, 945, 1136, 1230, 1347, 1737, 1842
TscAI CASTG 4 cut(s) 1221, 1945, 2030, 2191
TseFI GTSAC 2 cut(s) 19, 2049
TseI GCWGC 4 cut(s) 1834, 1837, 2119, 2215
Tsp45I GTSAC 2 cut(s) 19, 2049
TspDTI ATGAA 5 cut(s) 554, 657, 716, 1239, 1250
TspGWI ACGGA 3 cut(s) 167, 1569, 2121
TspRI CASTG 4 cut(s) 1221, 1945, 2030, 2191
Tth111I GACNNNGTC 1 cut(s) 35
Vha464I CTTAAG 2 cut(s) 1229, 1841
VpaK11BI GGWCC 3 cut(s) 147, 190, 960
VspI ATTAAT 1 cut(s) 1737
XapI RAATTY 5 cut(s) 430, 1289, 1910, 1944, 2082
XceI RCATGY 1 cut(s) 1176
XhoI CTCGAG 1 cut(s) 2045
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 2 cut(s) 710, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.