Rh3BG050300

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0016867
Physical Location & Seq
Forward (+)
3424498 .. 3427636
3139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG050300.1

Sequence Viewer

Length: 2229 bp
ATGGAAGAGACAGCTCCGGTGACGGCGAAAGACTCGGTCTTGAAGCGGCAGAGAAGCTTCGGCAAAGCGTACAAGACGTTCCAAACGCAGGCGACTTCGCTCGTCGGGTTGACCCGGCAGTTCAAGGAGCTCCAGGCGAGGTGGGGGTCCACGGAGGACGAGCTCCGGACGCGGTTCCGGGAAGTCAGGGACCGGGAAAGGGAGGTCGGCGCGAGAGAGAAGCAGTTGGAGGAGAAAGAGGCGAAGTGGAAGGTCGAGATGGAAGCGCAGGAGAAAGAGGTGGAATCGAGTAAGGACCGCTTGCTTTTCCTGAGGCCATTGATCAGTAAACATGTTCAAGAGCTTGATGGTTTGAAAGTGATGGTGAGTGAGAAGAAGAAAGAGCGTGATTTGATCGAAAAAAGGGGTTTAGAGTGGACTGAGAGATTGAATTCTGTTGAGAAATGTGTGGAGGAGAAGACGAAAGAGGTCGAGGCGAAGGAGGAGGAAGTGAGAGGGGTGGAAGGGAGGTTGAGGATTTATAGGGAGGAGATTGAGATGAAGGAGAGGGAGTTGAGTGGGATTTTGGAGACAATTGAGGAGTGCAAGAGAGAATGTGGTTTGAAAGAAGAGGAAATTGAAGCAAAACGAAGGTACATCGATGAATGGGAGAAGGAGATGGAATTGAAGAGAGAGGAACTTGTTGATCAGCTTGATGCTATGAAAATACTAGTTAGAGAGAAGGGGAAGGAGTGTGAGTTGATCGAAAATCGGGTTTTGGAGTCATCAACGAGATCGTATTATTTTGGGAAATGTGTAGAAGAGAGGAGGAAAGAGTTTGAGGCTAAGGAAAAGGAACTGAAAGAGGTTCAGGGGTGTATTGAGTTAAAGGAGAGGCAATTAGATGGGATTTTGAAGTCGATTCAGGAGCACACGAAAGAATGTGATTTGAAAGGAAAGCAAGTTAAGGCATTGGAGTGGTCCATTGAGAAATGTGAAAAAGAGATGGAATTGAAAAGGGAGGAGTGGTCTCGTAAGCTTGAGTTGAAGGAGATGGAACTAGAAAGGTTGTTTGACAACTTTGAGTTGAGAGGGCAACAACTTGAGCCGATTGTTGAAGAGCTTCGTGTTATTGATAAGAGGGTGGATGGATGCTTAAATGAGGGTCTGTGTAAAGAGAGGTACTTGCAGACATGTGAAAAATTGATACAAGAACGTGAAAGGTGTTTGGATTCAGTGTCCCATGAACTTAAGATGAAAGAGAGGCACATTGATGAGGAGGCCAAAGAGCTTGAATTGAAGCAAATAAAATTTTATTCTCAACTGAAGACTCAGCAGTTGGAAAGAGAAAAGCATTTTGCTTCACTTAAAAAATCGTTAGAACAACAAGAACAGTCTTTATATGCACTCCGAAAGTCGATAGAAGAGGTCAAAAAGCTTTTGGAGAGGCAACTTGAACAGCAGGGTGGAGTGATTGAATTGAAACAAAAACCATTTGATTCTCAGCTATTGGAAAGAGAAGAGCAACTTGGTTCAATTGGAAAGTCAATAGAAGAATGTGATAAGCAGTTCAACGGAGTTCAAAAGTTGGTTCAAGAGCAGAAAAAGCATTTGGATTCACTATCCCATACACTCCATATAAGAGAGACGCAAGCTGAAAAGCAGGGCAAACAGCTTGAATTGAAGTTTAGACATTCTGATTCTGAAGTGAATACTAAGCAGTTGGAACTCACCCCTGCCGCTGATGATATGATTATTCCTCACTCTATAAGTGATCAACCCAACATCAATAGGGATGGTAGAAACTTCGAGGTGATCGCTGGTATTATTCGGAATCTTATCGAAAGAAAGCAGCTGCTTAAGGCTGTTACATATATCTGCACCTTCAAGCAAATTGACAAGTTTCCCCCAGTACCACTCTTGAAAGAATTTGTGGAGGATGCAAAGAAGTCTTGCACTGAAATTTTCAGTAGAGAGATATCACATGATGTAAAGGAGAAGGTTGTAGATGACCTAATAGCTGATCTAGGAGCTCTGATTCAGTGCATTGAAGATCACAGCCTCGAGTCACAGTACCCACCAACTGACATTATGATGGAAATTCAAGGGCTTGGGGAACTAAAGGAGAATCCGAAGCTGCTATCTCTTCCGTCCAATGTTGAAAAACAAGAGCAGAGAAAAGGAAGGAAACGTAGTTCCAGCAGTGGGGGGAGCACATCTCACACATATCAGCTGCGTAAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

742

Amino Acids

87.5

Weight (kDa)

5.74

Isoelectric Point (pI)

55.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 599 - 705 3e-21 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016867)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04511 FvH4_6g04590 FvH4_6g04630
rosa_chinensis RchiOBHm_Chr3g0453101
rosa_laevigata RLG00000025537
rosa_multiflora Rmu_sc0009015.1_g000009
rosa_roxburghii Rroxscaffold_6G00430170
rosa_rugosa Rorug02G0646500 Rorug02G0646600
rosa_samantha Rh3AG049100 Rh3BG050300 Rh3CG050400 Rh3DG051100
rosa_wichuraiana Rw3G003760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 172, 212
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 4 cut(s) 46, 172, 298, 1719
AcsI RAATTY 5 cut(s) 430, 1289, 1907, 1941, 2079
AcuI CTGAAG 2 cut(s) 1325, 1704
AfaI GTAC 5 cut(s) 71, 635, 1163, 1893, 2054
AfiI CCNNNNNNNGG 3 cut(s) 88, 199, 2184
AflII CTTAAG 2 cut(s) 1229, 1838
AflIII ACRYGT 2 cut(s) 331, 1172
AhlI ACTAGT 1 cut(s) 709
AjnI CCWGG 1 cut(s) 132
AjuI GAANNNNNNNTTGG 4 cut(s) 1491, 1523, 1574, 1606
AloI GAACNNNNNNTCC 2 cut(s) 2158, 2190
Alw21I GWGCWC 5 cut(s) 132, 165, 912, 2014, 2195
Alw26I GTCTC 4 cut(s) 2, 563, 1014, 1619
Ama87I CYCGRG 1 cut(s) 2042
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 2 cut(s) 314, 1260
ApeKI GCWGC 4 cut(s) 1831, 1834, 2116, 2212
ApoI RAATTY 5 cut(s) 430, 1289, 1907, 1941, 2079
Asp700I GAANNNNTTC 1 cut(s) 1101
AspLEI GCGC 2 cut(s) 212, 268
AspS9I GGNCC 4 cut(s) 147, 190, 295, 960
AsuC2I CCSGG 3 cut(s) 115, 179, 194
AsuHPI GGTGA 4 cut(s) 31, 376, 1702, 1804
AvaI CYCGRG 1 cut(s) 2042
AvaII GGWCC 4 cut(s) 147, 190, 295, 960
AxyI CCTNAGG 1 cut(s) 311
BanII GRGCYC 3 cut(s) 132, 165, 2014
BbsI GAAGAC 2 cut(s) 464, 1313
Bbv12I GWGCWC 5 cut(s) 132, 165, 912, 2014, 2195
BbvI GCAGC 4 cut(s) 1821, 1843, 2103, 2199
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 134
BclI TGATCA 3 cut(s) 321, 685, 1753
BcnI CCSGG 3 cut(s) 115, 179, 194
BcoDI GTCTC 4 cut(s) 2, 563, 1014, 1619
BcuI ACTAGT 1 cut(s) 709
BfaI CTAG 3 cut(s) 710, 1040, 2006
BfrI CTTAAG 2 cut(s) 1229, 1838
BisI GCNGC 6 cut(s) 47, 1719, 1832, 1835, 2117, 2213
BlsI GCNGC 6 cut(s) 48, 1720, 1833, 1836, 2118, 2214
Bme1390I CCNGG 4 cut(s) 115, 134, 179, 194
Bme18I GGWCC 4 cut(s) 147, 190, 295, 960
BmeT110I CYCGRG 1 cut(s) 2042
BmgT120I GGNCC 4 cut(s) 147, 190, 295, 960
BmiI GGNNCC 3 cut(s) 148, 176, 191
BmrFI CCNGG 4 cut(s) 115, 134, 179, 194
BmrI ACTGGG 1 cut(s) 1883
BmsI GCATC 3 cut(s) 685, 1121, 1909
BmuI ACTGGG 1 cut(s) 1883
BpiI GAAGAC 2 cut(s) 464, 1313
BplI GAGNNNNNCTC 2 cut(s) 2182, 2214
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 825
BpuEI CTTGAG 2 cut(s) 1040, 1103
BpuMI CCSGG 3 cut(s) 115, 179, 194
Bsa29I ATCGAT 1 cut(s) 639
BsaI GGTCTC 1 cut(s) 1014
BsaJI CCNNGG 1 cut(s) 150
BsaWI WCCGGW 2 cut(s) 16, 165
BsaXI ACNNNNNCTCC 6 cut(s) 536, 566, 722, 752, 992, 1022
Bsc4I CCNNNNNNNGG 3 cut(s) 88, 199, 2184
Bse1I ACTGG 1 cut(s) 1889
Bse21I CCTNAGG 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 1 cut(s) 134
BseCI ATCGAT 1 cut(s) 639
BseDI CCNNGG 1 cut(s) 150
BseGI GGATG 4 cut(s) 1132, 1136, 1780, 1924
BseLI CCNNNNNNNGG 3 cut(s) 88, 199, 2184
BseMII CTCAG 4 cut(s) 302, 411, 1325, 1496
BseNI ACTGG 1 cut(s) 1889
BseRI GAGGAG 8 cut(s) 245, 467, 497, 542, 593, 820, 1016, 1271
BseXI GCAGC 4 cut(s) 1821, 1843, 2103, 2199
BsgI GTGCAG 1 cut(s) 1843
Bsh1236I CGCG 2 cut(s) 172, 212
BshFI GGCC 2 cut(s) 316, 1262
BshVI ATCGAT 1 cut(s) 639
BsiHKAI GWGCWC 5 cut(s) 132, 165, 912, 2014, 2195
BsiHKCI CYCGRG 1 cut(s) 2042
BsiSI CCGG 5 cut(s) 17, 115, 166, 178, 193
BslFI GGGAC 2 cut(s) 203, 1204
BslI CCNNNNNNNGG 3 cut(s) 88, 199, 2184
BsmAI GTCTC 4 cut(s) 2, 563, 1014, 1619
BsmBI CGTCTC 1 cut(s) 1619
BsmFI GGGAC 2 cut(s) 203, 1204
BsnI GGCC 2 cut(s) 316, 1262
Bso31I GGTCTC 1 cut(s) 1014
BsoBI CYCGRG 1 cut(s) 2042
Bsp1286I GDGCHC 5 cut(s) 132, 165, 912, 2014, 2195
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
BspACI CCGC 4 cut(s) 46, 172, 298, 1719
BspANI GGCC 2 cut(s) 316, 1262
BspCNI CTCAG 4 cut(s) 303, 412, 1324, 1495
BspDI ATCGAT 1 cut(s) 639
BspEI TCCGGA 1 cut(s) 165
BspFNI CGCG 2 cut(s) 172, 212
BspLI GGNNCC 3 cut(s) 148, 176, 191
BspQI GCTCTTC 2 cut(s) 1092, 1494
BspTI CTTAAG 2 cut(s) 1229, 1838
BspTNI GGTCTC 1 cut(s) 1014
BsrI ACTGG 1 cut(s) 1889
BssECI CCNNGG 1 cut(s) 150
BssMI GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
Bst2UI CCWGG 1 cut(s) 134
Bst4CI ACNGT 2 cut(s) 1374, 2052
Bst6I CTCTTC 7 cut(s) 603, 662, 795, 1092, 1398, 1494, 2130
BstAFI CTTAAG 2 cut(s) 1229, 1838
BstC8I GCNNGC 3 cut(s) 90, 302, 1632
BstDEI CTNAG 6 cut(s) 311, 420, 825, 1311, 1482, 1695
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 4 cut(s) 1132, 1136, 1780, 1924
BstFNI CGCG 2 cut(s) 172, 212
BstHHI GCGC 2 cut(s) 212, 268
BstKTI GATC 9 cut(s) 324, 396, 688, 744, 776, 1756, 1797, 2005, 2035
BstMAI GTCTC 4 cut(s) 2, 563, 1014, 1619
BstMBI GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
BstMWI GCNNNNNNNGC 2 cut(s) 169, 1585
BstNI CCWGG 1 cut(s) 134
BstNSI RCATGY 2 cut(s) 335, 1176
BstSCI CCNGG 4 cut(s) 113, 132, 177, 192
BstUI CGCG 2 cut(s) 172, 212
BstV1I GCAGC 4 cut(s) 1821, 1843, 2103, 2199
BstV2I GAAGAC 2 cut(s) 464, 1313
Bsu15I ATCGAT 1 cut(s) 639
Bsu36I CCTNAGG 1 cut(s) 311
BsuRI GGCC 2 cut(s) 316, 1262
BsuTUI ATCGAT 1 cut(s) 639
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 4 cut(s) 1132, 1136, 1780, 1924
BtsI GCAGTG 1 cut(s) 2188
BtsIMutI CAGTG 4 cut(s) 1221, 1935, 2027, 2188
Cac8I GCNNGC 3 cut(s) 90, 302, 1632
CfoI GCGC 2 cut(s) 212, 268
Cfr13I GGNCC 4 cut(s) 147, 190, 295, 960
ClaI ATCGAT 1 cut(s) 639
CseI GACGC 2 cut(s) 178, 1636
Csp6I GTAC 5 cut(s) 70, 634, 1162, 1892, 2053
CviAII CATG 4 cut(s) 332, 1173, 1223, 1964
CviQI GTAC 5 cut(s) 70, 634, 1162, 1892, 2053
DdeI CTNAG 6 cut(s) 311, 420, 825, 1311, 1482, 1695
DpnI GATC 9 cut(s) 323, 395, 687, 743, 775, 1755, 1796, 2004, 2034
DpnII GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
Eam1104I CTCTTC 7 cut(s) 603, 662, 795, 1092, 1398, 1494, 2130
EarI CTCTTC 7 cut(s) 603, 662, 795, 1092, 1398, 1494, 2130
Ecl136II GAGCTC 3 cut(s) 130, 163, 2012
Eco24I GRGCYC 3 cut(s) 132, 165, 2014
Eco31I GGTCTC 1 cut(s) 1014
Eco32I GATATC 1 cut(s) 1959
Eco47I GGWCC 4 cut(s) 147, 190, 295, 960
Eco53kI GAGCTC 3 cut(s) 130, 163, 2012
Eco57I CTGAAG 2 cut(s) 1325, 1704
Eco81I CCTNAGG 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 2042
EcoICRI GAGCTC 3 cut(s) 130, 163, 2012
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 132
EcoRV GATATC 1 cut(s) 1959
EcoT38I GRGCYC 3 cut(s) 132, 165, 2014
Esp3I CGTCTC 1 cut(s) 1619
FaeI CATG 4 cut(s) 335, 1176, 1226, 1967
FalI AAGNNNNNCTT 4 cut(s) 284, 316, 1491, 1523
FaqI GGGAC 2 cut(s) 203, 1204
FatI CATG 4 cut(s) 331, 1172, 1222, 1963
FbaI TGATCA 3 cut(s) 321, 685, 1753
Fnu4HI GCNGC 6 cut(s) 47, 1719, 1832, 1835, 2117, 2213
FokI GGATG 4 cut(s) 1139, 1143, 1787, 1931
FriOI GRGCYC 3 cut(s) 132, 165, 2014
Fsp4HI GCNGC 6 cut(s) 47, 1719, 1832, 1835, 2117, 2213
FspBI CTAG 3 cut(s) 710, 1040, 2006
GlaI GCGC 2 cut(s) 211, 267
GluI GCNGC 6 cut(s) 47, 1719, 1832, 1835, 2117, 2213
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 2 cut(s) 316, 1262
HapII CCGG 5 cut(s) 17, 115, 166, 178, 193
HgaI GACGC 2 cut(s) 178, 1636
HhaI GCGC 2 cut(s) 212, 268
Hin1II CATG 4 cut(s) 335, 1176, 1226, 1967
Hin6I GCGC 2 cut(s) 210, 266
HinP1I GCGC 2 cut(s) 210, 266
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 3 cut(s) 55, 1016, 1415
HpaII CCGG 5 cut(s) 17, 115, 166, 178, 193
HphI GGTGA 4 cut(s) 31, 376, 1702, 1804
Hpy166II GTNNAC 4 cut(s) 111, 150, 329, 417
Hpy188I TCNGA 6 cut(s) 1391, 1678, 1684, 1812, 2016, 2112
Hpy188III TCNNGA 8 cut(s) 40, 166, 256, 310, 338, 905, 1574, 1900
Hpy8I GTNNAC 4 cut(s) 111, 150, 329, 417
Hpy99I CGWCG 1 cut(s) 107
HpyCH4III ACNGT 2 cut(s) 1374, 2052
HpyCH4IV ACGT 3 cut(s) 77, 1195, 2170
HpyCH4V TGCA 7 cut(s) 585, 1168, 1385, 1860, 1922, 1935, 2025
HpyF10VI GCNNNNNNNGC 2 cut(s) 169, 1585
HpyF3I CTNAG 6 cut(s) 311, 420, 825, 1311, 1482, 1695
HpySE526I ACGT 3 cut(s) 77, 1195, 2170
Hsp92II CATG 4 cut(s) 335, 1176, 1226, 1967
HspAI GCGC 2 cut(s) 210, 266
Kpn2I TCCGGA 1 cut(s) 165
Ksp22I TGATCA 3 cut(s) 321, 685, 1753
Kzo9I GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
LguI GCTCTTC 2 cut(s) 1092, 1494
LmnI GCTCC 7 cut(s) 19, 127, 135, 168, 907, 2009, 2190
Lsp1109I GCAGC 4 cut(s) 1821, 1843, 2103, 2199
LweI GCATC 3 cut(s) 685, 1121, 1909
MaeI CTAG 3 cut(s) 710, 1040, 2006
MaeII ACGT 3 cut(s) 77, 1195, 2170
MaeIII GTNAC 3 cut(s) 19, 1846, 2046
MalI GATC 9 cut(s) 323, 395, 687, 743, 775, 1755, 1796, 2004, 2034
MboI GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
MfeI CAATTG 2 cut(s) 573, 1515
MhlI GDGCHC 5 cut(s) 132, 165, 912, 2014, 2195
MlyI GAGTC 4 cut(s) 26, 770, 1303, 2054
MmeI TCCRAC 3 cut(s) 207, 1299, 1683
MroI TCCGGA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 1101
MseI TTAA 6 cut(s) 866, 945, 1136, 1230, 1347, 1839
MslI CAYNNNNRTG 3 cut(s) 955, 1251, 2072
MspA1I CMGCKG 3 cut(s) 1721, 1834, 2212
MspCI CTTAAG 2 cut(s) 1229, 1838
MspI CCGG 5 cut(s) 17, 115, 166, 178, 193
MspR9I CCNGG 4 cut(s) 115, 134, 179, 194
MunI CAATTG 2 cut(s) 573, 1515
MvaI CCWGG 1 cut(s) 134
MvnI CGCG 2 cut(s) 172, 212
MwoI GCNNNNNNNGC 2 cut(s) 169, 1585
NciI CCSGG 3 cut(s) 115, 179, 194
NdeII GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
NlaIII CATG 4 cut(s) 335, 1176, 1226, 1967
NlaIV GGNNCC 3 cut(s) 148, 176, 191
NmuCI GTSAC 2 cut(s) 19, 2046
NspI RCATGY 2 cut(s) 335, 1176
PaeR7I CTCGAG 1 cut(s) 2042
PciI ACATGT 2 cut(s) 331, 1172
PciSI GCTCTTC 2 cut(s) 1092, 1494
PdmI GAANNNNTTC 1 cut(s) 1101
PfeI GAWTC 9 cut(s) 284, 901, 1211, 1478, 1595, 1679, 1813, 2017, 2107
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 6 cut(s) 48, 1720, 1833, 1836, 2118, 2214
PleI GAGTC 4 cut(s) 26, 769, 1303, 2053
PpsI GAGTC 4 cut(s) 26, 769, 1303, 2053
PscI ACATGT 2 cut(s) 331, 1172
Psp124BI GAGCTC 3 cut(s) 132, 165, 2014
Psp6I CCWGG 1 cut(s) 132
PspGI CCWGG 1 cut(s) 132
PspN4I GGNNCC 3 cut(s) 148, 176, 191
PspPI GGNCC 4 cut(s) 147, 190, 295, 960
PspXI VCTCGAGB 1 cut(s) 2042
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 2 cut(s) 1834, 2212
RsaI GTAC 5 cut(s) 71, 635, 1163, 1893, 2054
RsaNI GTAC 5 cut(s) 70, 634, 1162, 1892, 2053
RseI CAYNNNNRTG 3 cut(s) 955, 1251, 2072
SacI GAGCTC 3 cut(s) 132, 165, 2014
SapI GCTCTTC 2 cut(s) 1092, 1494
SaqAI TTAA 6 cut(s) 866, 945, 1136, 1230, 1347, 1839
SatI GCNGC 6 cut(s) 47, 1719, 1832, 1835, 2117, 2213
Sau3AI GATC 9 cut(s) 321, 393, 685, 741, 773, 1753, 1794, 2002, 2032
Sau96I GGNCC 4 cut(s) 147, 190, 295, 960
SchI GAGTC 4 cut(s) 26, 770, 1303, 2054
ScrFI CCNGG 4 cut(s) 115, 134, 179, 194
SduI GDGCHC 5 cut(s) 132, 165, 912, 2014, 2195
SfaNI GCATC 3 cut(s) 685, 1121, 1909
Sfr274I CTCGAG 1 cut(s) 2042
SinI GGWCC 4 cut(s) 147, 190, 295, 960
SlaI CTCGAG 1 cut(s) 2042
SmiMI CAYNNNNRTG 3 cut(s) 955, 1251, 2072
SmlI CTYRAG 5 cut(s) 1019, 1082, 1229, 1838, 2042
SmoI CTYRAG 5 cut(s) 1019, 1082, 1229, 1838, 2042
SpeI ACTAGT 1 cut(s) 709
SsiI CCGC 4 cut(s) 46, 172, 298, 1719
SspMI CTAG 3 cut(s) 710, 1040, 2006
SstI GAGCTC 3 cut(s) 132, 165, 2014
StyD4I CCNGG 4 cut(s) 113, 132, 177, 192
TaaI ACNGT 2 cut(s) 1374, 2052
TaiI ACGT 3 cut(s) 80, 1198, 2173
TaqII GACCGA 1 cut(s) 25
TauI GCSGC 2 cut(s) 49, 1721
TfiI GAWTC 9 cut(s) 284, 901, 1211, 1478, 1595, 1679, 1813, 2017, 2107
Tru1I TTAA 6 cut(s) 866, 945, 1136, 1230, 1347, 1839
Tru9I TTAA 6 cut(s) 866, 945, 1136, 1230, 1347, 1839
TscAI CASTG 4 cut(s) 1221, 1942, 2027, 2188
TseFI GTSAC 2 cut(s) 19, 2046
TseI GCWGC 4 cut(s) 1831, 1834, 2116, 2212
Tsp45I GTSAC 2 cut(s) 19, 2046
TspDTI ATGAA 5 cut(s) 554, 657, 716, 1239, 1250
TspGWI ACGGA 3 cut(s) 167, 1569, 2118
TspRI CASTG 4 cut(s) 1221, 1942, 2027, 2188
Tth111I GACNNNGTC 1 cut(s) 35
Vha464I CTTAAG 2 cut(s) 1229, 1838
VpaK11BI GGWCC 4 cut(s) 147, 190, 295, 960
XapI RAATTY 5 cut(s) 430, 1289, 1907, 1941, 2079
XceI RCATGY 2 cut(s) 335, 1176
XhoI CTCGAG 1 cut(s) 2042
XmnI GAANNNNTTC 1 cut(s) 1101
XspI CTAG 3 cut(s) 710, 1040, 2006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.