Rw3G003760

Frigida-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0016867
Physical Location & Seq
Forward (+)
3248656 .. 3251548
2893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003760.1

Sequence Viewer

Length: 2160 bp
ATGGAAGAGACAGCTCCGGTGACGGCGAAAGACTCGGTCTTGAAGCGGCAGAGAAGCTTCGGCAAAGCGTACAAGACGTTCCAAACGCAGGCGACTTCGCTCGTCGGGTTGACCCGGCAGTTCAAGGAGCTCCAGGCGCGGTGGGGGTCCACGGAGGACGAGCTCCGGACGCGGTTCAGGGAAGTCCGGGACCGGGAAAGGGAGGTCGGCGCGAGAGAGAAGCAGTTGGAGGAGAAAGAGGCGAAGTGGAAGGTCGAGATGGAAGCGCAGAAGAAAGAGGTGGAATCGAGTAAGCACCAGTTGCTTTTCCTGAGGCCATTGATCAGTAAACGTGTTCAAGAGCTTGATGGTTTGAAAGTGATGGTGAGTGAGAAGAAGAAAGAGCGTGATTTGATCGAAAAAAGGGGTTTAGAGTGGACTGAGAGATTGAATTCTGTTGAGAAATGTGTGGAGGAGAAGACGAAAGAGGTCGAGGCGAAGGAGGAGGAAGTGAGAGGGGTGGAAGGGAGGTTGAGGATTTATAGGGAGGAGATTGAGATGAAGGAGAGGGAGTTGAGTGGGATTTTGGGGTCAATTGAGGAGTGCAGGAGAGAATGTGGTTTGAAAGAAGAGGAAATTAAGCGAGCGAAGCTTGATGCTATGAAAATACTAGTTAGAGAGAAGGGGAAGGAGTGTGAGTTGATCGAAAATCGGGTTTTGGAGTCATCAACGAGATCGTATTATTTTGGGAAATGTGTAGAAGAGAGGAGGAAAGAGTTTGAGGCTAAGGAAAAGGAACTGAAAGAGGTTCAGGGGTGTATTGAGTTGAAGGAGAGGCAATTAGATGGGATTTTGAAGTCGATTCAGGAGCACACGAAAGAATGTGATTTGAAAGGAAAGCAAGTTAAGGCATTATTGGAGTGGTCCATTGAGAAATGTGAAAAAGAGATGGAATTGAAAAGGGAGGAGTGGTCTCGTAAGCTTGAGTTGAAGGAGATGGAACTAGAAAGGTTGTTTGACAAGTTTGAGTTGAGAGGGCAACAACTTGAGCCGATAGTTGAAGAGCTTCGTGTTATTGAAAAGAGGGTGGATGGATGCTTAAATAAGGGTCTGTGTAAAGAGAGGTACTTGCAGACATGTGAAAAATTGATACAAGAACGCGAAAGGTGTTTGGATTCAGTGTCCCATGAACTTAAGATGAAAGAGAGGCACATTGATGAGGAGGCCAAAGAGCTTGAATTGAAGCAAATAAAACTGAAGACTCAGCAGTTGGAAAGAGAAAAGCATTTGGCTTCACTTAAAAAATCGTTAGAACAACAAGAACAGTCTTTATATGCACTCCGAAAGTCGATAGAAGAGGTCAAAAAGCTTTTGGAGAGGCAACTTGAACAGCAGGGTGGAGTGATTGAATTGAAACAAAAACCATTTGATTCTCAGCTATTGGAAAGAGAAGGGCAACTTGGTTCAATTGGAAAGTCAATAGAAGAATGTGATAAGCAGTTCAATGGAGTTCAAAAGTTGGTTCAAGAGCAGAAAAAGCATTTGGATTCACTATCCCATACACTCCATATCAGAGAGACGCAAGCTGAAAAGCAGGGCAAACAGCTTGAATTGAAGTTTAGACATTCTGATTCTGAAGTGAATACCAAGCAGTTGGAACTCACCCCTGCCGCTGATGATATGATTAATCCTCACTCTATAAGTGATCAACCCAACATCAATAGGGATGGTAGAAGCTTCGAGGTGATCCCTGGTATTATTCGGAATCTTATCGAAAGAAAGCAGCTGCTTAAGGCTGTTACATATATCTGCACCTTCAAGCAAATTGACAAGTTTCCCCCAGTTCCACTCTTGCAAGAATTTGTGGAGGATGCAAAGAAGTCTTGCACTGAAATTTTCAGTAGAGAGATATCACATGATGTAAAGGAGAAGGTTGTAGATGACCTAATAGCTGATCTAAGAGCTCTGATTCAGTGCATTGAAGATCACAGCCTCGAGTCACAGTACCCACCAACTACCATTATGATGGAAATTCAAGGGCTTGGGGAACTAAAGGAGAATCCGAAGCTGCTATCTCTTCCGTCCAATGTTGAAAAACAAGAGCAGAGAAAAGGAAGGAAACGAAGTTCCAGCAGTGGGGGGAGCACATCTCACACATATCAGCTGCGTAAAAGAACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

719

Amino Acids

84.42

Weight (kDa)

7.19

Isoelectric Point (pI)

57.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 575 - 682 4.3e-22 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016867)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g04511 FvH4_6g04590 FvH4_6g04630
rosa_chinensis RchiOBHm_Chr3g0453101
rosa_laevigata RLG00000025537
rosa_multiflora Rmu_sc0009015.1_g000009
rosa_roxburghii Rroxscaffold_6G00430170
rosa_rugosa Rorug02G0646500 Rorug02G0646600
rosa_samantha Rh3AG049100 Rh3BG050300 Rh3CG050400 Rh3DG051100
rosa_wichuraiana Rw3G003760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 139, 172, 212, 1140
AccIII TCCGGA 1 cut(s) 165
AciI CCGC 4 cut(s) 46, 139, 172, 1650
AclWI GGATC 1 cut(s) 1720
AcsI RAATTY 4 cut(s) 430, 1838, 1872, 2010
AcuI CTGAAG 2 cut(s) 1256, 1635
AfaI GTAC 3 cut(s) 71, 1106, 1985
AfiI CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2115
AflII CTTAAG 2 cut(s) 1172, 1769
AflIII ACRYGT 2 cut(s) 331, 1115
AhlI ACTAGT 1 cut(s) 649
AjnI CCWGG 2 cut(s) 132, 1729
AjuI GAANNNNNNNTTGG 4 cut(s) 1422, 1454, 1505, 1537
AloI GAACNNNNNNTCC 2 cut(s) 2089, 2121
Alw21I GWGCWC 5 cut(s) 132, 165, 852, 1945, 2126
Alw26I GTCTC 3 cut(s) 2, 957, 1550
AlwI GGATC 1 cut(s) 1720
Ama87I CYCGRG 1 cut(s) 1973
Aor13HI TCCGGA 1 cut(s) 165
AoxI GGCC 2 cut(s) 314, 1203
ApeKI GCWGC 4 cut(s) 1762, 1765, 2047, 2143
ApoI RAATTY 4 cut(s) 430, 1838, 1872, 2010
AseI ATTAAT 1 cut(s) 1665
Asp700I GAANNNNTTC 1 cut(s) 1044
AspLEI GCGC 3 cut(s) 139, 212, 268
AspS9I GGNCC 3 cut(s) 147, 190, 903
AsuC2I CCSGG 3 cut(s) 115, 188, 194
AsuHPI GGTGA 4 cut(s) 31, 376, 1633, 1735
AvaI CYCGRG 1 cut(s) 1973
AvaII GGWCC 3 cut(s) 147, 190, 903
AxyI CCTNAGG 1 cut(s) 311
BanII GRGCYC 3 cut(s) 132, 165, 1945
BbsI GAAGAC 2 cut(s) 464, 1244
Bbv12I GWGCWC 5 cut(s) 132, 165, 852, 1945, 2126
BbvI GCAGC 4 cut(s) 1752, 1774, 2034, 2130
BccI CCATC 9 cut(s) 253, 341, 355, 818, 922, 970, 1064, 1700, 1999
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 2 cut(s) 134, 1731
BclI TGATCA 2 cut(s) 321, 1684
BcnI CCSGG 3 cut(s) 115, 188, 194
BcoDI GTCTC 3 cut(s) 2, 957, 1550
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 2 cut(s) 650, 983
BfrI CTTAAG 2 cut(s) 1172, 1769
BisI GCNGC 6 cut(s) 47, 1650, 1763, 1766, 2048, 2144
BlsI GCNGC 6 cut(s) 48, 1651, 1764, 1767, 2049, 2145
Bme1390I CCNGG 5 cut(s) 115, 134, 188, 194, 1731
Bme18I GGWCC 3 cut(s) 147, 190, 903
BmeT110I CYCGRG 1 cut(s) 1973
BmgT120I GGNCC 3 cut(s) 147, 190, 903
BmiI GGNNCC 2 cut(s) 148, 191
BmrFI CCNGG 5 cut(s) 115, 134, 188, 194, 1731
BmrI ACTGGG 1 cut(s) 1814
BmsI GCATC 3 cut(s) 625, 1064, 1840
BmuI ACTGGG 1 cut(s) 1814
BpiI GAAGAC 2 cut(s) 464, 1244
BplI GAGNNNNNCTC 2 cut(s) 2113, 2145
BpmI CTGGAG 1 cut(s) 116
Bpu10I CCTNAGC 1 cut(s) 765
BpuEI CTTGAG 2 cut(s) 983, 1046
BpuMI CCSGG 3 cut(s) 115, 188, 194
BsaI GGTCTC 1 cut(s) 957
BsaJI CCNNGG 2 cut(s) 150, 1729
BsaWI WCCGGW 2 cut(s) 16, 165
BsaXI ACNNNNNCTCC 8 cut(s) 536, 566, 580, 610, 662, 692, 935, 965
Bsc4I CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2115
Bse1I ACTGG 2 cut(s) 298, 1820
Bse21I CCTNAGG 1 cut(s) 311
BseAI TCCGGA 1 cut(s) 165
BseBI CCWGG 2 cut(s) 134, 1731
BseDI CCNNGG 2 cut(s) 150, 1729
BseGI GGATG 4 cut(s) 1075, 1079, 1711, 1855
BseLI CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2115
BseMII CTCAG 4 cut(s) 302, 411, 1256, 1427
BseNI ACTGG 2 cut(s) 298, 1820
BseRI GAGGAG 8 cut(s) 245, 467, 497, 542, 593, 760, 959, 1214
BseXI GCAGC 4 cut(s) 1752, 1774, 2034, 2130
BsgI GTGCAG 2 cut(s) 604, 1774
Bsh1236I CGCG 4 cut(s) 139, 172, 212, 1140
BshFI GGCC 2 cut(s) 316, 1205
BsiHKAI GWGCWC 5 cut(s) 132, 165, 852, 1945, 2126
BsiHKCI CYCGRG 1 cut(s) 1973
BsiSI CCGG 5 cut(s) 17, 115, 166, 187, 193
BslFI GGGAC 2 cut(s) 203, 1147
BslI CCNNNNNNNGG 4 cut(s) 88, 193, 199, 2115
BsmAI GTCTC 3 cut(s) 2, 957, 1550
BsmBI CGTCTC 1 cut(s) 1550
BsmFI GGGAC 2 cut(s) 203, 1147
BsnI GGCC 2 cut(s) 316, 1205
Bso31I GGTCTC 1 cut(s) 957
BsoBI CYCGRG 1 cut(s) 1973
Bsp1286I GDGCHC 5 cut(s) 132, 165, 852, 1945, 2126
Bsp13I TCCGGA 1 cut(s) 165
Bsp143I GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
BspACI CCGC 4 cut(s) 46, 139, 172, 1650
BspANI GGCC 2 cut(s) 316, 1205
BspCNI CTCAG 4 cut(s) 303, 412, 1255, 1426
BspEI TCCGGA 1 cut(s) 165
BspFNI CGCG 4 cut(s) 139, 172, 212, 1140
BspLI GGNNCC 2 cut(s) 148, 191
BspPI GGATC 1 cut(s) 1720
BspQI GCTCTTC 1 cut(s) 1035
BspTI CTTAAG 2 cut(s) 1172, 1769
BspTNI GGTCTC 1 cut(s) 957
BsrI ACTGG 2 cut(s) 298, 1820
BssECI CCNNGG 2 cut(s) 150, 1729
BssMI GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
Bst2UI CCWGG 2 cut(s) 134, 1731
Bst4CI ACNGT 2 cut(s) 1305, 1983
Bst6I CTCTTC 5 cut(s) 603, 735, 1035, 1329, 2061
BstAFI CTTAAG 2 cut(s) 1172, 1769
BstAPI GCANNNNNTGC 1 cut(s) 301
BstC8I GCNNGC 3 cut(s) 90, 624, 1563
BstDEI CTNAG 6 cut(s) 311, 420, 765, 1242, 1413, 1937
BstDSI CCRYGG 1 cut(s) 150
BstF5I GGATG 4 cut(s) 1075, 1079, 1711, 1855
BstFNI CGCG 4 cut(s) 139, 172, 212, 1140
BstHHI GCGC 3 cut(s) 139, 212, 268
BstKTI GATC 8 cut(s) 324, 396, 684, 716, 1687, 1728, 1936, 1966
BstMAI GTCTC 3 cut(s) 2, 957, 1550
BstMBI GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
BstMWI GCNNNNNNNGC 5 cut(s) 136, 169, 301, 628, 1516
BstNI CCWGG 2 cut(s) 134, 1731
BstNSI RCATGY 1 cut(s) 1119
BstSCI CCNGG 5 cut(s) 113, 132, 186, 192, 1729
BstUI CGCG 4 cut(s) 139, 172, 212, 1140
BstV1I GCAGC 4 cut(s) 1752, 1774, 2034, 2130
BstV2I GAAGAC 2 cut(s) 464, 1244
BstXI CCANNNNNNTGG 2 cut(s) 1633, 2005
Bsu36I CCTNAGG 1 cut(s) 311
BsuRI GGCC 2 cut(s) 316, 1205
BtgI CCRYGG 1 cut(s) 150
BtsCI GGATG 4 cut(s) 1075, 1079, 1711, 1855
BtsI GCAGTG 1 cut(s) 2119
BtsIMutI CAGTG 4 cut(s) 1164, 1866, 1958, 2119
Cac8I GCNNGC 3 cut(s) 90, 624, 1563
CfoI GCGC 3 cut(s) 139, 212, 268
Cfr13I GGNCC 3 cut(s) 147, 190, 903
CseI GACGC 2 cut(s) 178, 1567
Csp6I GTAC 3 cut(s) 70, 1105, 1984
CviAII CATG 3 cut(s) 1116, 1166, 1895
CviQI GTAC 3 cut(s) 70, 1105, 1984
DdeI CTNAG 6 cut(s) 311, 420, 765, 1242, 1413, 1937
DpnI GATC 8 cut(s) 323, 395, 683, 715, 1686, 1727, 1935, 1965
DpnII GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
Eam1104I CTCTTC 5 cut(s) 603, 735, 1035, 1329, 2061
EarI CTCTTC 5 cut(s) 603, 735, 1035, 1329, 2061
Ecl136II GAGCTC 3 cut(s) 130, 163, 1943
Eco24I GRGCYC 3 cut(s) 132, 165, 1945
Eco31I GGTCTC 1 cut(s) 957
Eco32I GATATC 1 cut(s) 1890
Eco47I GGWCC 3 cut(s) 147, 190, 903
Eco53kI GAGCTC 3 cut(s) 130, 163, 1943
Eco57I CTGAAG 2 cut(s) 1256, 1635
Eco81I CCTNAGG 1 cut(s) 311
Eco88I CYCGRG 1 cut(s) 1973
EcoICRI GAGCTC 3 cut(s) 130, 163, 1943
EcoRI GAATTC 1 cut(s) 430
EcoRII CCWGG 2 cut(s) 132, 1729
EcoRV GATATC 1 cut(s) 1890
EcoT38I GRGCYC 3 cut(s) 132, 165, 1945
Esp3I CGTCTC 1 cut(s) 1550
FaeI CATG 3 cut(s) 1119, 1169, 1898
FalI AAGNNNNNCTT 2 cut(s) 1422, 1454
FaqI GGGAC 2 cut(s) 203, 1147
FatI CATG 3 cut(s) 1115, 1165, 1894
FbaI TGATCA 2 cut(s) 321, 1684
Fnu4HI GCNGC 6 cut(s) 47, 1650, 1763, 1766, 2048, 2144
FokI GGATG 4 cut(s) 1082, 1086, 1718, 1862
FriOI GRGCYC 3 cut(s) 132, 165, 1945
Fsp4HI GCNGC 6 cut(s) 47, 1650, 1763, 1766, 2048, 2144
FspBI CTAG 2 cut(s) 650, 983
GlaI GCGC 3 cut(s) 138, 211, 267
GluI GCNGC 6 cut(s) 47, 1650, 1763, 1766, 2048, 2144
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 2 cut(s) 316, 1205
HapII CCGG 5 cut(s) 17, 115, 166, 187, 193
HgaI GACGC 2 cut(s) 178, 1567
HhaI GCGC 3 cut(s) 139, 212, 268
Hin1II CATG 3 cut(s) 1119, 1169, 1898
Hin6I GCGC 3 cut(s) 137, 210, 266
HinP1I GCGC 3 cut(s) 137, 210, 266
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 5 cut(s) 55, 629, 959, 1346, 1714
HpaII CCGG 5 cut(s) 17, 115, 166, 187, 193
HphI GGTGA 4 cut(s) 31, 376, 1633, 1735
Hpy166II GTNNAC 4 cut(s) 111, 150, 329, 417
Hpy188I TCNGA 7 cut(s) 1322, 1553, 1609, 1615, 1743, 1947, 2043
Hpy188III TCNNGA 7 cut(s) 40, 166, 256, 310, 338, 845, 1505
Hpy8I GTNNAC 4 cut(s) 111, 150, 329, 417
Hpy99I CGWCG 1 cut(s) 107
HpyCH4III ACNGT 2 cut(s) 1305, 1983
HpyCH4IV ACGT 2 cut(s) 77, 331
HpyCH4V TGCA 8 cut(s) 585, 1111, 1316, 1791, 1834, 1853, 1866, 1956
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 169, 301, 628, 1516
HpyF3I CTNAG 6 cut(s) 311, 420, 765, 1242, 1413, 1937
HpySE526I ACGT 2 cut(s) 77, 331
Hsp92II CATG 3 cut(s) 1119, 1169, 1898
HspAI GCGC 3 cut(s) 137, 210, 266
Kpn2I TCCGGA 1 cut(s) 165
Ksp22I TGATCA 2 cut(s) 321, 1684
Kzo9I GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
LguI GCTCTTC 1 cut(s) 1035
LmnI GCTCC 6 cut(s) 19, 127, 135, 168, 847, 2121
Lsp1109I GCAGC 4 cut(s) 1752, 1774, 2034, 2130
LweI GCATC 3 cut(s) 625, 1064, 1840
MaeI CTAG 2 cut(s) 650, 983
MaeII ACGT 2 cut(s) 77, 331
MaeIII GTNAC 3 cut(s) 19, 1777, 1977
MalI GATC 8 cut(s) 323, 395, 683, 715, 1686, 1727, 1935, 1965
MboI GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
MfeI CAATTG 2 cut(s) 573, 1446
MhlI GDGCHC 5 cut(s) 132, 165, 852, 1945, 2126
MlyI GAGTC 4 cut(s) 26, 710, 1234, 1985
MmeI TCCRAC 3 cut(s) 207, 1230, 1614
MroI TCCGGA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 1044
MseI TTAA 7 cut(s) 618, 885, 1079, 1173, 1278, 1665, 1770
MslI CAYNNNNRTG 2 cut(s) 1194, 2003
MspA1I CMGCKG 3 cut(s) 1652, 1765, 2143
MspCI CTTAAG 2 cut(s) 1172, 1769
MspI CCGG 5 cut(s) 17, 115, 166, 187, 193
MspR9I CCNGG 5 cut(s) 115, 134, 188, 194, 1731
MunI CAATTG 2 cut(s) 573, 1446
MvaI CCWGG 2 cut(s) 134, 1731
MvnI CGCG 4 cut(s) 139, 172, 212, 1140
MwoI GCNNNNNNNGC 5 cut(s) 136, 169, 301, 628, 1516
NciI CCSGG 3 cut(s) 115, 188, 194
NdeII GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
NlaIII CATG 3 cut(s) 1119, 1169, 1898
NlaIV GGNNCC 2 cut(s) 148, 191
NmuCI GTSAC 2 cut(s) 19, 1977
NspI RCATGY 1 cut(s) 1119
PaeR7I CTCGAG 1 cut(s) 1973
PciI ACATGT 1 cut(s) 1115
PciSI GCTCTTC 1 cut(s) 1035
PdmI GAANNNNTTC 1 cut(s) 1044
PfeI GAWTC 9 cut(s) 284, 841, 1154, 1409, 1526, 1610, 1744, 1948, 2038
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 6 cut(s) 48, 1651, 1764, 1767, 2049, 2145
PleI GAGTC 4 cut(s) 26, 709, 1234, 1984
PpsI GAGTC 4 cut(s) 26, 709, 1234, 1984
PscI ACATGT 1 cut(s) 1115
PshBI ATTAAT 1 cut(s) 1665
Psp124BI GAGCTC 3 cut(s) 132, 165, 1945
Psp6I CCWGG 2 cut(s) 132, 1729
PspGI CCWGG 2 cut(s) 132, 1729
PspN4I GGNNCC 2 cut(s) 148, 191
PspPI GGNCC 3 cut(s) 147, 190, 903
PspXI VCTCGAGB 1 cut(s) 1973
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 2 cut(s) 1765, 2143
RsaI GTAC 3 cut(s) 71, 1106, 1985
RsaNI GTAC 3 cut(s) 70, 1105, 1984
RseI CAYNNNNRTG 2 cut(s) 1194, 2003
SacI GAGCTC 3 cut(s) 132, 165, 1945
SapI GCTCTTC 1 cut(s) 1035
SaqAI TTAA 7 cut(s) 618, 885, 1079, 1173, 1278, 1665, 1770
SatI GCNGC 6 cut(s) 47, 1650, 1763, 1766, 2048, 2144
Sau3AI GATC 8 cut(s) 321, 393, 681, 713, 1684, 1725, 1933, 1963
Sau96I GGNCC 3 cut(s) 147, 190, 903
SchI GAGTC 4 cut(s) 26, 710, 1234, 1985
ScrFI CCNGG 5 cut(s) 115, 134, 188, 194, 1731
SduI GDGCHC 5 cut(s) 132, 165, 852, 1945, 2126
SfaNI GCATC 3 cut(s) 625, 1064, 1840
Sfr274I CTCGAG 1 cut(s) 1973
SinI GGWCC 3 cut(s) 147, 190, 903
SlaI CTCGAG 1 cut(s) 1973
SmiMI CAYNNNNRTG 2 cut(s) 1194, 2003
SmlI CTYRAG 5 cut(s) 962, 1025, 1172, 1769, 1973
SmoI CTYRAG 5 cut(s) 962, 1025, 1172, 1769, 1973
SpeI ACTAGT 1 cut(s) 649
SsiI CCGC 4 cut(s) 46, 139, 172, 1650
SspMI CTAG 2 cut(s) 650, 983
SstI GAGCTC 3 cut(s) 132, 165, 1945
StyD4I CCNGG 5 cut(s) 113, 132, 186, 192, 1729
TaaI ACNGT 2 cut(s) 1305, 1983
TaiI ACGT 2 cut(s) 80, 334
TaqII GACCGA 1 cut(s) 25
TauI GCSGC 2 cut(s) 49, 1652
TfiI GAWTC 9 cut(s) 284, 841, 1154, 1409, 1526, 1610, 1744, 1948, 2038
Tru1I TTAA 7 cut(s) 618, 885, 1079, 1173, 1278, 1665, 1770
Tru9I TTAA 7 cut(s) 618, 885, 1079, 1173, 1278, 1665, 1770
TscAI CASTG 4 cut(s) 1164, 1873, 1958, 2119
TseFI GTSAC 2 cut(s) 19, 1977
TseI GCWGC 4 cut(s) 1762, 1765, 2047, 2143
Tsp45I GTSAC 2 cut(s) 19, 1977
TspDTI ATGAA 4 cut(s) 554, 656, 1182, 1193
TspGWI ACGGA 2 cut(s) 167, 2049
TspRI CASTG 4 cut(s) 1164, 1873, 1958, 2119
Tth111I GACNNNGTC 1 cut(s) 35
Vha464I CTTAAG 2 cut(s) 1172, 1769
VpaK11BI GGWCC 3 cut(s) 147, 190, 903
VspI ATTAAT 1 cut(s) 1665
XapI RAATTY 4 cut(s) 430, 1838, 1872, 2010
XceI RCATGY 1 cut(s) 1119
XhoI CTCGAG 1 cut(s) 1973
XmnI GAANNNNTTC 1 cut(s) 1044
XspI CTAG 2 cut(s) 650, 983
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.