RchiOBHm_Chr3g0486021

S-adenosylmethionine-dependent methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018848
Physical Location & Seq
Reverse (-)
32932605 .. 32933136
532 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45056

Sequence Viewer

Length: 441 bp
ATGGAGTGCTTTCTGCATGCCAGGGCACAAGAGACAGTACATGGAGGATTGATGGTACTTGTTACTCCTGGTTACCTAGCTGATACACCTCCTTCTCATACTCTGGCAAATGTCACCTATCAAATTTTAGGATCTTGCCTCATTGACATGGCTAGGAAGGGAGTAGTCAATGAAGAGAAAATAGATTCATTTAATGTACCTATATACTATGTGTGTCCCCGAGAACTGGAAGATGTTGTAGAACAAAATGGATGTTTTAGCATAGAGATAATGGAACACTTACCTACTATGATGGAATCTGACACTATTTCAAAAAATAGCAAACATGTTAGAGCTATCATGGAGGGGCTCTTCATGCAGCATTTCGGAGAAGAAATCTTAGATGAGCTCTTCGACTTGTTTCACACGAAAGTAAAAGAGCAGGACTCCGTGTTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_7

Protein Analysis

146

Amino Acids

16.58

Weight (kDa)

4.73

Isoelectric Point (pI)

37.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 1 - 141 4.3e-37 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018848)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g16980
rosa_chinensis RchiOBHm_Chr3g0486021
rosa_laevigata RLG00000023111
rosa_multiflora Rmu_co8379655.1_g000001 Rmu_sc0006229.1_g000003
rosa_samantha Rh3CG306600 Rh3DG302800 Rh5CG266800 Rh5CG570100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 139
AcsI RAATTY 1 cut(s) 123
AfaI GTAC 3 cut(s) 39, 57, 198
AfiI CCNNNNNNNGG 1 cut(s) 226
AflIII ACRYGT 1 cut(s) 325
AgsI TTSAA 1 cut(s) 312
AjnI CCWGG 2 cut(s) 20, 67
AluBI AGCT 3 cut(s) 80, 335, 388
AluI AGCT 3 cut(s) 80, 335, 388
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 26
AlwI GGATC 1 cut(s) 139
Ama87I CYCGRG 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 358
ApoI RAATTY 1 cut(s) 123
AsuHPI GGTGA 1 cut(s) 106
AvaI CYCGRG 1 cut(s) 219
BaeGI GKGCMC 1 cut(s) 28
BanII GRGCYC 2 cut(s) 351, 390
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 370
BccI CCATC 2 cut(s) 46, 286
BciT130I CCWGG 2 cut(s) 22, 69
BcoDI GTCTC 1 cut(s) 26
BfaI CTAG 2 cut(s) 77, 153
BisI GCNGC 1 cut(s) 359
BlsI GCNGC 1 cut(s) 360
Bme1390I CCNGG 2 cut(s) 22, 69
BmeT110I CYCGRG 1 cut(s) 219
BmrFI CCNGG 2 cut(s) 22, 69
BplI GAGNNNNNCTC 1 cut(s) 410
BsaJI CCNNGG 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 231
BseBI CCWGG 2 cut(s) 22, 69
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 257
BseLI CCNNNNNNNGG 1 cut(s) 226
BseNI ACTGG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 28
BseXI GCAGC 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 219
BslFI GGGAC 1 cut(s) 201
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 1 cut(s) 26
BsmFI GGGAC 1 cut(s) 201
BsoBI CYCGRG 1 cut(s) 219
Bsp1286I GDGCHC 3 cut(s) 28, 351, 390
Bsp143I GATC 1 cut(s) 131
BspPI GGATC 1 cut(s) 139
BspQI GCTCTTC 2 cut(s) 356, 395
BsrI ACTGG 1 cut(s) 231
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 1 cut(s) 131
Bst2UI CCWGG 2 cut(s) 22, 69
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 3 cut(s) 168, 356, 395
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 379
BstEII GGTNACC 1 cut(s) 71
BstF5I GGATG 1 cut(s) 257
BstKTI GATC 1 cut(s) 134
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 131
BstMWI GCNNNNNNNGC 1 cut(s) 355
BstNI CCWGG 2 cut(s) 22, 69
BstNSI RCATGY 2 cut(s) 20, 329
BstPI GGTNACC 1 cut(s) 71
BstSCI CCNGG 2 cut(s) 20, 67
BstSLI GKGCMC 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 370
BstX2I RGATCY 1 cut(s) 131
BstYI RGATCY 1 cut(s) 131
BtsCI GGATG 1 cut(s) 257
Cac8I GCNNGC 1 cut(s) 18
Csp6I GTAC 3 cut(s) 38, 56, 197
CviAII CATG 6 cut(s) 17, 41, 148, 326, 340, 355
CviJI RGCY 5 cut(s) 80, 152, 335, 349, 388
CviKI_1 RGCY 5 cut(s) 80, 152, 335, 349, 388
CviQI GTAC 3 cut(s) 38, 56, 197
DdeI CTNAG 1 cut(s) 379
DpnI GATC 1 cut(s) 133
DpnII GATC 1 cut(s) 131
Eam1104I CTCTTC 3 cut(s) 168, 356, 395
EarI CTCTTC 3 cut(s) 168, 356, 395
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 2 cut(s) 351, 390
Eco53kI GAGCTC 1 cut(s) 388
Eco88I CYCGRG 1 cut(s) 219
Eco91I GGTNACC 1 cut(s) 71
EcoICRI GAGCTC 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 71
EcoRII CCWGG 2 cut(s) 20, 67
EcoT38I GRGCYC 2 cut(s) 351, 390
FaeI CATG 6 cut(s) 20, 44, 151, 329, 343, 358
FaqI GGGAC 1 cut(s) 201
FatI CATG 6 cut(s) 16, 40, 147, 325, 339, 354
Fnu4HI GCNGC 1 cut(s) 359
FokI GGATG 1 cut(s) 264
FriOI GRGCYC 2 cut(s) 351, 390
Fsp4HI GCNGC 1 cut(s) 359
FspBI CTAG 2 cut(s) 77, 153
GluI GCNGC 1 cut(s) 359
Hin1II CATG 6 cut(s) 20, 44, 151, 329, 343, 358
HinfI GANTC 3 cut(s) 185, 296, 425
HphI GGTGA 1 cut(s) 106
Hpy188I TCNGA 2 cut(s) 301, 368
HpyAV CCTTC 2 cut(s) 102, 151
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 2 cut(s) 16, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 355
HpyF3I CTNAG 1 cut(s) 379
Hsp92II CATG 6 cut(s) 20, 44, 151, 329, 343, 358
Kzo9I GATC 1 cut(s) 131
LguI GCTCTTC 2 cut(s) 356, 395
LpnPI CCDG 7 cut(s) 7, 34, 54, 81, 89, 212, 407
Lsp1109I GCAGC 1 cut(s) 370
MaeI CTAG 2 cut(s) 77, 153
MaeIII GTNAC 3 cut(s) 61, 71, 112
MalI GATC 1 cut(s) 133
MboI GATC 1 cut(s) 131
MboII GAAGA 5 cut(s) 185, 242, 343, 382, 383
MflI RGATCY 1 cut(s) 131
MhlI GDGCHC 3 cut(s) 28, 351, 390
MluCI AATT 1 cut(s) 123
MlyI GAGTC 1 cut(s) 419
MnlI CCTC 4 cut(s) 38, 99, 149, 337
MseI TTAA 1 cut(s) 192
MslI CAYNNNNRTG 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 22, 69
MvaI CCWGG 2 cut(s) 22, 69
MwoI GCNNNNNNNGC 1 cut(s) 355
NdeII GATC 1 cut(s) 131
NlaIII CATG 6 cut(s) 20, 44, 151, 329, 343, 358
NmuCI GTSAC 1 cut(s) 112
NspI RCATGY 2 cut(s) 20, 329
PaeI GCATGC 1 cut(s) 20
PciI ACATGT 1 cut(s) 325
PciSI GCTCTTC 2 cut(s) 356, 395
PfeI GAWTC 2 cut(s) 185, 296
PkrI GCNGC 1 cut(s) 360
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PscI ACATGT 1 cut(s) 325
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 2 cut(s) 20, 67
PspEI GGTNACC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 20, 67
PsuI RGATCY 1 cut(s) 131
RsaI GTAC 3 cut(s) 39, 57, 198
RsaNI GTAC 3 cut(s) 38, 56, 197
RseI CAYNNNNRTG 1 cut(s) 146
SacI GAGCTC 1 cut(s) 390
SapI GCTCTTC 2 cut(s) 356, 395
SaqAI TTAA 1 cut(s) 192
SatI GCNGC 1 cut(s) 359
Sau3AI GATC 1 cut(s) 131
SchI GAGTC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 22, 69
SduI GDGCHC 3 cut(s) 28, 351, 390
SetI ASST 8 cut(s) 78, 82, 91, 119, 202, 286, 337, 390
SmiMI CAYNNNNRTG 1 cut(s) 146
SphI GCATGC 1 cut(s) 20
Sse9I AATT 1 cut(s) 123
SspMI CTAG 2 cut(s) 77, 153
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 2 cut(s) 20, 67
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 1 cut(s) 393
TasI AATT 1 cut(s) 123
TatI WGTACW 1 cut(s) 37
TfiI GAWTC 2 cut(s) 185, 296
Tru1I TTAA 1 cut(s) 192
Tru9I TTAA 1 cut(s) 192
TseFI GTSAC 1 cut(s) 112
TseI GCWGC 1 cut(s) 358
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 3 cut(s) 177, 186, 343
TspGWI ACGGA 1 cut(s) 418
XapI RAATTY 1 cut(s) 123
XceI RCATGY 2 cut(s) 20, 329
XspI CTAG 2 cut(s) 77, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.