RchiOBHm_Chr3g0488701
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0014188
Physical Location & Seq
Forward (+)
36350273 .. 36354292
4020 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ45302

Sequence Viewer

Length: 1554 bp
ATGATTACCTCACAGCTAAGAGGAAGAGGAAGCAAACCCATACCCATCACCAAAATCGCCAAAGTCTTCCTCCAAAACCCACACAAACTCACTCCCATATCCCCACCAACCCAATCCCACCAGAACCCATGTGCCCCACTCTCCCATACCCAAAACCCTTTATGGGTTTCACAGAGCTGTGACTATTCAACGCACTCCTCTAAGTTTCCCGAGTATGAAATGCCCTCAGTCACTTGGGGTGTGGTCCAAGGCCGCAAAGAGAAGCTTGTCTCTAGAGTCATAATATGTGACTATTTGAAGAGTATAGGCATAGTTCCCGATGAATTGGAAAACTTGGAGCTACCCTCCACTGTTGATGTCATGAGGGAAAGAGTTGAGTTCCTACAGAAGCTAGGATTGTCGATTGACGACATTAACGAGTACCCTTTGATGCTCGGATGCAGTGTCAGGAAAAACATGATACCCGTGTTGGCTTACTTGGAGAAAATTGGGATTCAGAGGTCGAAGCTAGGTGAATTTGTTAAGAATTACCCGCAAGTGTTACATGCTAGTGTGGTAGTTGAGCTTGTTCCGGTGATCAAGTTCCTTCGTGGGCTCGATGTGGAGAAGCTGGATATTGGCTATGTCTTGCAGAAGTATCCAGAGCTTCTCGGATATAAGCTTGAGGGGACTATGAGTACTTCTGTTGCGTATCTTGTTAGTATTGGTGTTACTCCTAGGGATATAGGTCCCATGGTCACCCAGTATCCTTATCTATTGGGCATGAGAGTTGGGACTGTGATTAAGCCTTTTGTTGATTATTTGGTTTCCTTAGGTTTGCCGAAAAAGATTTTGGCTAGGATGTTGGAGAAGCGCGCATATTTACTTGGTTATGATCTTGAGGAGACTATTAAGCCTAATGTGGATTGTTTGGTTGCTTTTGGGATCAGGAGGGAAGCACTTGCTTCTATTGTTGCTCAATACCCCCAAATTCTTGGTCAGCCTTTGAAAGCTAAAATGTCTTCACAGCAGTTTTTCTTCAGTATGAAGCTTAAAATTGATCCTGAAGGGTTTGCACGGGTGATAGAGAAGATGCCGCAGATAGTCAGCCTTCATCAACATGTGATAATGAAGCCTGTTGAGTTCCTTCTTGGACGGGGGATACCTTCAGAAGATGTAGCTAAGATGGTCGTAAGGTGTCCCCAATTAGTGGCACTGCGTGTTGAGCTCATGAAGAACAGTTATTACTTTTTTAAGAGTGAAATGGGGAGACCAGTGAAAGAGCTTGTGGAATTTCCGGAATACTTTACATATAGTTTAGAATCCAGGATCAAACCTAGATACCAGAGGTTGCAAAGCAAGGGGATTAGGTGTTCTTTGAAATGGTTCCTCAACTGTAGCGATCAGAGATTTGAGGAGAGGTTGCAAGGGGAGTACATTGAAACAGAGATTTCCGGTCCATCATTTTGTATGGGAGGGAAGTTAGAGCTACCTGGGGTTGGAAATGAGATGGTCTCAGATGGGGAAGACGAGAGTGATGATGAAATACTTTACAGACGCACTGTATCCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

517

Amino Acids

58.75

Weight (kDa)

8.62

Isoelectric Point (pI)

39.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 157 - 465 4.7e-113 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44020
fragaria_vesca FvH4_1g27350 FvH4_1g27350 FvH4_1g27350 FvH4_1g27350
malus_domestica MD07G1031000.v1.1
prunus_persica Prupe.8G049300_v2.0.a1
pyrus_communis pycom07g02260
rosa_chinensis RchiOBHm_Chr3g0488701
rosa_laevigata RLG00000022930
rosa_multiflora Rmu_sc0001208.1_g000078
rosa_samantha Rh3AG289100 Rh3BG325900 Rh3CG323400 Rh3DG321900
rosa_wichuraiana Rw0G013690 Rw3G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1189
AccII CGCG 1 cut(s) 855
AccIII TCCGGA 1 cut(s) 1276
AciI CCGC 3 cut(s) 253, 533, 1076
AclWI GGATC 3 cut(s) 932, 1034, 1316
AcsI RAATTY 3 cut(s) 515, 969, 1271
AcuI CTGAAG 3 cut(s) 1003, 1065, 1131
AdeI CACNNNGTG 1 cut(s) 1199
AfaI GTAC 3 cut(s) 422, 679, 1415
AfiI CCNNNNNNNGG 3 cut(s) 163, 1189, 1478
AflIII ACRYGT 1 cut(s) 1099
AgsI TTSAA 5 cut(s) 189, 298, 988, 1360, 1421
AjnI CCWGG 2 cut(s) 1304, 1471
AjuI GAANNNNNNNTTGG 2 cut(s) 815, 847
AloI GAACNNNNNNTCC 2 cut(s) 1336, 1368
Alw21I GWGCWC 1 cut(s) 1209
Alw26I GTCTC 4 cut(s) 274, 878, 1243, 1498
AlwI GGATC 3 cut(s) 932, 1034, 1316
Ama87I CYCGRG 1 cut(s) 209
Aor13HI TCCGGA 1 cut(s) 1276
AoxI GGCC 1 cut(s) 250
ApoI RAATTY 3 cut(s) 515, 969, 1271
ArsI GACNNNNNNTTYG 2 cut(s) 961, 993
Asp700I GAANNNNTTC 1 cut(s) 1364
AspA2I CCTAGG 1 cut(s) 716
AspLEI GCGC 2 cut(s) 855, 857
AspS9I GGNCC 3 cut(s) 244, 728, 1436
AsuHPI GGTGA 5 cut(s) 40, 524, 586, 730, 1072
AvaI CYCGRG 1 cut(s) 209
AvaII GGWCC 3 cut(s) 244, 728, 1436
AvrII CCTAGG 1 cut(s) 716
AxyI CCTNAGG 1 cut(s) 811
BaeGI GKGCMC 1 cut(s) 136
BaeI ACNNNNGTAYC 2 cut(s) 452, 485
BanII GRGCYC 2 cut(s) 597, 1209
BbsI GAAGAC 3 cut(s) 58, 993, 1512
Bbv12I GWGCWC 1 cut(s) 1209
BccI CCATC 5 cut(s) 53, 1159, 1447, 1483, 1493
BciT130I CCWGG 2 cut(s) 1306, 1473
BciVI GTATCC 3 cut(s) 648, 756, 1134
BclI TGATCA 1 cut(s) 576
BcoDI GTCTC 4 cut(s) 274, 878, 1243, 1498
BfaI CTAG 7 cut(s) 273, 392, 509, 549, 717, 837, 1317
BfmI CTRYAG 3 cut(s) 383, 1375, 1550
BfuI GTATCC 3 cut(s) 648, 756, 1134
BisI GCNGC 2 cut(s) 253, 1076
BlnI CCTAGG 1 cut(s) 716
BlsI GCNGC 2 cut(s) 254, 1077
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 2 cut(s) 1306, 1473
Bme18I GGWCC 3 cut(s) 244, 728, 1436
BmeT110I CYCGRG 1 cut(s) 209
BmgT120I GGNCC 3 cut(s) 244, 728, 1436
BmiI GGNNCC 2 cut(s) 730, 1367
BmrFI CCNGG 2 cut(s) 1306, 1473
BmrI ACTGGG 1 cut(s) 736
BmsI GCATC 3 cut(s) 420, 428, 1062
BmuI ACTGGG 1 cut(s) 736
BpiI GAAGAC 3 cut(s) 58, 993, 1512
BplI GAGNNNNNCTC 4 cut(s) 329, 361, 1478, 1510
BpuEI CTTGAG 2 cut(s) 683, 899
BsaI GGTCTC 2 cut(s) 1243, 1498
BsaJI CCNNGG 4 cut(s) 247, 716, 732, 1472
BsaWI WCCGGW 3 cut(s) 571, 1276, 1433
Bsc4I CCNNNNNNNGG 3 cut(s) 163, 1189, 1478
Bse1I ACTGG 2 cut(s) 742, 1253
Bse21I CCTNAGG 1 cut(s) 811
BseAI TCCGGA 1 cut(s) 1276
BseBI CCWGG 2 cut(s) 1306, 1473
BseDI CCNNGG 4 cut(s) 247, 716, 732, 1472
BseGI GGATG 2 cut(s) 443, 846
BseLI CCNNNNNNNGG 3 cut(s) 163, 1189, 1478
BseMII CTCAG 2 cut(s) 240, 1509
BseNI ACTGG 2 cut(s) 742, 1253
BsePI GCGCGC 1 cut(s) 853
BseRI GAGGAG 3 cut(s) 187, 896, 1409
BseSI GKGCMC 1 cut(s) 136
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 1 cut(s) 252
BsiHKAI GWGCWC 1 cut(s) 1209
BsiHKCI CYCGRG 1 cut(s) 209
BsiSI CCGG 3 cut(s) 572, 1277, 1434
BslFI GGGAC 4 cut(s) 682, 714, 787, 1164
BslI CCNNNNNNNGG 3 cut(s) 163, 1189, 1478
BsmAI GTCTC 4 cut(s) 274, 878, 1243, 1498
BsmFI GGGAC 4 cut(s) 682, 714, 787, 1164
BsnI GGCC 1 cut(s) 252
Bso31I GGTCTC 2 cut(s) 1243, 1498
BsoBI CYCGRG 1 cut(s) 209
Bsp1286I GDGCHC 3 cut(s) 136, 597, 1209
Bsp13I TCCGGA 1 cut(s) 1276
Bsp143I GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
Bsp19I CCATGG 1 cut(s) 732
BspACI CCGC 3 cut(s) 253, 533, 1076
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 2 cut(s) 239, 1508
BspEI TCCGGA 1 cut(s) 1276
BspFNI CGCG 1 cut(s) 855
BspHI TCATGA 2 cut(s) 360, 1209
BspLI GGNNCC 2 cut(s) 730, 1367
BspPI GGATC 3 cut(s) 932, 1034, 1316
BspTNI GGTCTC 2 cut(s) 1243, 1498
BsrI ACTGG 2 cut(s) 742, 1253
BssECI CCNNGG 4 cut(s) 247, 716, 732, 1472
BssHII GCGCGC 1 cut(s) 853
BssMI GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
BssT1I CCWWGG 3 cut(s) 247, 716, 732
Bst2UI CCWGG 2 cut(s) 1306, 1473
Bst4CI ACNGT 5 cut(s) 352, 778, 1220, 1376, 1543
Bst6I CTCTTC 2 cut(s) 19, 293
BstC8I GCNNGC 1 cut(s) 855
BstDEI CTNAG 6 cut(s) 17, 201, 226, 811, 1161, 1495
BstDSI CCRYGG 1 cut(s) 732
BstEII GGTNACC 1 cut(s) 736
BstF5I GGATG 2 cut(s) 443, 846
BstFNI CGCG 1 cut(s) 855
BstHHI GCGC 2 cut(s) 855, 857
BstKTI GATC 6 cut(s) 579, 877, 927, 1042, 1311, 1384
BstMAI GTCTC 4 cut(s) 274, 878, 1243, 1498
BstMBI GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
BstMWI GCNNNNNNNGC 1 cut(s) 1204
BstNI CCWGG 2 cut(s) 1306, 1473
BstNSI RCATGY 2 cut(s) 548, 1103
BstPI GGTNACC 1 cut(s) 736
BstSCI CCNGG 2 cut(s) 1304, 1471
BstSFI CTRYAG 3 cut(s) 383, 1375, 1550
BstSLI GKGCMC 1 cut(s) 136
BstUI CGCG 1 cut(s) 855
BstV2I GAAGAC 3 cut(s) 58, 993, 1512
BstXI CCANNNNNNTGG 1 cut(s) 974
Bsu36I CCTNAGG 1 cut(s) 811
BsuI GTATCC 3 cut(s) 648, 756, 1134
BsuRI GGCC 1 cut(s) 252
BtgI CCRYGG 1 cut(s) 732
BtsCI GGATG 2 cut(s) 443, 846
BtsI GCAGTG 2 cut(s) 448, 1193
BtsIMutI CAGTG 5 cut(s) 348, 448, 1193, 1260, 1539
Cac8I GCNNGC 1 cut(s) 855
CciI TCATGA 2 cut(s) 360, 1209
CfoI GCGC 2 cut(s) 855, 857
Cfr13I GGNCC 3 cut(s) 244, 728, 1436
CseI GACGC 1 cut(s) 1545
Csp6I GTAC 3 cut(s) 421, 678, 1414
CviAII CATG 8 cut(s) 129, 361, 457, 545, 733, 763, 1100, 1210
CviQI GTAC 3 cut(s) 421, 678, 1414
DdeI CTNAG 6 cut(s) 17, 201, 226, 811, 1161, 1495
DpnI GATC 6 cut(s) 578, 876, 926, 1041, 1310, 1383
DpnII GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
DraIII CACNNNGTG 1 cut(s) 1199
Eam1104I CTCTTC 2 cut(s) 19, 293
EarI CTCTTC 2 cut(s) 19, 293
Ecl136II GAGCTC 1 cut(s) 1207
Eco130I CCWWGG 3 cut(s) 247, 716, 732
Eco24I GRGCYC 2 cut(s) 597, 1209
Eco31I GGTCTC 2 cut(s) 1243, 1498
Eco47I GGWCC 3 cut(s) 244, 728, 1436
Eco53kI GAGCTC 1 cut(s) 1207
Eco57I CTGAAG 3 cut(s) 1003, 1065, 1131
Eco81I CCTNAGG 1 cut(s) 811
Eco88I CYCGRG 1 cut(s) 209
Eco91I GGTNACC 1 cut(s) 736
EcoICRI GAGCTC 1 cut(s) 1207
EcoO109I RGGNCCY 1 cut(s) 728
EcoO65I GGTNACC 1 cut(s) 736
EcoRII CCWGG 2 cut(s) 1304, 1471
EcoT14I CCWWGG 3 cut(s) 247, 716, 732
EcoT38I GRGCYC 2 cut(s) 597, 1209
ErhI CCWWGG 3 cut(s) 247, 716, 732
FaeI CATG 8 cut(s) 132, 364, 460, 548, 736, 766, 1103, 1213
FalI AAGNNNNNCTT 2 cut(s) 249, 281
FaqI GGGAC 4 cut(s) 682, 714, 787, 1164
FatI CATG 8 cut(s) 128, 360, 456, 544, 732, 762, 1099, 1209
FauI CCCGC 1 cut(s) 540
FbaI TGATCA 1 cut(s) 576
Fnu4HI GCNGC 2 cut(s) 253, 1076
FokI GGATG 2 cut(s) 450, 853
FriOI GRGCYC 2 cut(s) 597, 1209
Fsp4HI GCNGC 2 cut(s) 253, 1076
FspBI CTAG 7 cut(s) 273, 392, 509, 549, 717, 837, 1317
GlaI GCGC 2 cut(s) 854, 856
GluI GCNGC 2 cut(s) 253, 1076
HaeIII GGCC 1 cut(s) 252
HapII CCGG 3 cut(s) 572, 1277, 1434
HgaI GACGC 1 cut(s) 1545
HhaI GCGC 2 cut(s) 855, 857
Hin1II CATG 8 cut(s) 132, 364, 460, 548, 736, 766, 1103, 1213
Hin6I GCGC 2 cut(s) 853, 855
HinP1I GCGC 2 cut(s) 853, 855
HindIII AAGCTT 3 cut(s) 263, 659, 1028
HinfI GANTC 3 cut(s) 276, 493, 1301
HpaII CCGG 3 cut(s) 572, 1277, 1434
HphI GGTGA 5 cut(s) 40, 524, 586, 730, 1072
Hpy188I TCNGA 6 cut(s) 437, 498, 653, 1150, 1386, 1498
HpyAV CCTTC 5 cut(s) 596, 1040, 1100, 1136, 1155
HpyCH4III ACNGT 5 cut(s) 352, 778, 1220, 1376, 1543
HpyCH4V TGCA 5 cut(s) 441, 631, 1055, 1333, 1405
HpyF10VI GCNNNNNNNGC 1 cut(s) 1204
HpyF3I CTNAG 6 cut(s) 17, 201, 226, 811, 1161, 1495
Hsp92II CATG 8 cut(s) 132, 364, 460, 548, 736, 766, 1103, 1213
HspAI GCGC 2 cut(s) 853, 855
Kpn2I TCCGGA 1 cut(s) 1276
Ksp22I TGATCA 1 cut(s) 576
Kzo9I GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
LmnI GCTCC 1 cut(s) 337
LweI GCATC 3 cut(s) 420, 428, 1062
MaeI CTAG 7 cut(s) 273, 392, 509, 549, 717, 837, 1317
MaeIII GTNAC 6 cut(s) 179, 229, 287, 540, 709, 736
MalI GATC 6 cut(s) 578, 876, 926, 1041, 1310, 1383
MboI GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
MboII GAAGA 9 cut(s) 36, 58, 310, 993, 1009, 1081, 1163, 1225, 1517
MhlI GDGCHC 3 cut(s) 136, 597, 1209
MluCI AATT 8 cut(s) 323, 486, 515, 526, 969, 1035, 1184, 1271
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 2 cut(s) 825, 1459
MroI TCCGGA 1 cut(s) 1276
MroXI GAANNNNTTC 1 cut(s) 1364
MseI TTAA 6 cut(s) 414, 522, 783, 891, 1032, 1233
MslI CAYNNNNRTG 2 cut(s) 549, 1098
MspI CCGG 3 cut(s) 572, 1277, 1434
MspR9I CCNGG 2 cut(s) 1306, 1473
MvaI CCWGG 2 cut(s) 1306, 1473
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 1 cut(s) 1204
NcoI CCATGG 1 cut(s) 732
NdeII GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
NlaIII CATG 8 cut(s) 132, 364, 460, 548, 736, 766, 1103, 1213
NlaIV GGNNCC 2 cut(s) 730, 1367
NmuCI GTSAC 4 cut(s) 179, 229, 287, 736
NspI RCATGY 2 cut(s) 548, 1103
PagI TCATGA 2 cut(s) 360, 1209
PauI GCGCGC 1 cut(s) 853
PciI ACATGT 1 cut(s) 1099
PcsI WCGNNNNNNNCGW 1 cut(s) 414
PdmI GAANNNNTTC 1 cut(s) 1364
PfeI GAWTC 2 cut(s) 493, 1301
PflMI CCANNNNNTGG 1 cut(s) 1189
PfoI TCCNGGA 1 cut(s) 1304
PkrI GCNGC 2 cut(s) 254, 1077
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
PpuMI RGGWCCY 1 cut(s) 728
PscI ACATGT 1 cut(s) 1099
Psp124BI GAGCTC 1 cut(s) 1209
Psp5II RGGWCCY 1 cut(s) 728
Psp6I CCWGG 2 cut(s) 1304, 1471
PspEI GGTNACC 1 cut(s) 736
PspGI CCWGG 2 cut(s) 1304, 1471
PspN4I GGNNCC 2 cut(s) 730, 1367
PspPI GGNCC 3 cut(s) 244, 728, 1436
PspPPI RGGWCCY 1 cut(s) 728
PsrI GAACNNNNNNTAC 2 cut(s) 1208, 1240
PteI GCGCGC 1 cut(s) 853
RsaI GTAC 3 cut(s) 422, 679, 1415
RsaNI GTAC 3 cut(s) 421, 678, 1414
RseI CAYNNNNRTG 2 cut(s) 549, 1098
SacI GAGCTC 1 cut(s) 1209
SaqAI TTAA 6 cut(s) 414, 522, 783, 891, 1032, 1233
SatI GCNGC 2 cut(s) 253, 1076
Sau3AI GATC 6 cut(s) 576, 874, 924, 1039, 1308, 1381
Sau96I GGNCC 3 cut(s) 244, 728, 1436
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 1 cut(s) 285
ScrFI CCNGG 2 cut(s) 1306, 1473
SduI GDGCHC 3 cut(s) 136, 597, 1209
SfaNI GCATC 3 cut(s) 420, 428, 1062
SfcI CTRYAG 3 cut(s) 383, 1375, 1550
SinI GGWCC 3 cut(s) 244, 728, 1436
SmiMI CAYNNNNRTG 2 cut(s) 549, 1098
SmlI CTYRAG 2 cut(s) 662, 878
SmoI CTYRAG 2 cut(s) 662, 878
Sse9I AATT 8 cut(s) 323, 486, 515, 526, 969, 1035, 1184, 1271
SsiI CCGC 3 cut(s) 253, 533, 1076
SspMI CTAG 7 cut(s) 273, 392, 509, 549, 717, 837, 1317
SstI GAGCTC 1 cut(s) 1209
StyD4I CCNGG 2 cut(s) 1304, 1471
StyI CCWWGG 3 cut(s) 247, 716, 732
TaaI ACNGT 5 cut(s) 352, 778, 1220, 1376, 1543
TaqI TCGA 3 cut(s) 401, 503, 597
TasI AATT 8 cut(s) 323, 486, 515, 526, 969, 1035, 1184, 1271
TatI WGTACW 2 cut(s) 677, 1413
TauI GCSGC 2 cut(s) 255, 1078
TfiI GAWTC 2 cut(s) 493, 1301
Tru1I TTAA 6 cut(s) 414, 522, 783, 891, 1032, 1233
Tru9I TTAA 6 cut(s) 414, 522, 783, 891, 1032, 1233
TscAI CASTG 5 cut(s) 355, 448, 1200, 1260, 1546
TseFI GTSAC 4 cut(s) 179, 229, 287, 736
Tsp45I GTSAC 4 cut(s) 179, 229, 287, 736
TspDTI ATGAA 7 cut(s) 231, 336, 1040, 1082, 1124, 1226, 1536
TspRI CASTG 5 cut(s) 355, 448, 1200, 1260, 1546
Van91I CCANNNNNTGG 1 cut(s) 1189
VpaK11BI GGWCC 3 cut(s) 244, 728, 1436
XapI RAATTY 3 cut(s) 515, 969, 1271
XbaI TCTAGA 1 cut(s) 272
XceI RCATGY 2 cut(s) 548, 1103
XmaJI CCTAGG 1 cut(s) 716
XmnI GAANNNNTTC 1 cut(s) 1364
XspI CTAG 7 cut(s) 273, 392, 509, 549, 717, 837, 1317
ZrmI AGTACT 1 cut(s) 679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.