Rh3DG321900
ERF Family

mTERF

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0014188
Physical Location & Seq
Forward (+)
34986452 .. 34991371
4920 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG321900.1

Sequence Viewer

Length: 1548 bp
ATGATTACCTCGCAGCTAAGAGGAAGAGGAAGCAAACCCATCACCAAAATCGCCAAAGTCTTCCTCCAAAACCCACACAAACTCGCTCCCATATCCCCACCAACCCAATCCCACCAGAACCCATGTGCCCCACTCTCCCATACCCAAACCCCTTTATGGGTTTCACAGAGCTGTGACTACTCAACGCAGTCCTCTAAGTTTCCCGAGTATGAAATGCCCTCAGTCACTTGGGGCGTGGTCCAAGGCCGCAAAGAGAAGCTTGTCTCTAGAGTCATAATATGTGACTATTTGAAGAGTATAGGCATAGTTCCCGATGAATTGGAAAACTTGGAGCTACCCTCTACTGTTGATGTCATGAGGGAAAGAGTTGAGTTCCTACAGAAGCTAGGATTGTCGATTGACGACATTAACGAGTACCCTTTGATGCTCGGATGCAGTGTCAGGAAAAACATGATACCCGTGTTGGCTTACTTGGAGAAAATTGGGATTCAGAGGTCGAAGCTAGGTGAATTTGTTAAGAATTACCCACAAGTGTTACATGCTAGTGTGGTAGTTGAGCTTGTTCCGGTGATCAAGTTCCTTCGTGGGCTCGATGTGGAGAAGCTGGATATTGGCTATGTCTTGCAGAAGTATCCAGAGCTTCTCGGATATAAGCTTGAGGGGACTATGAGTACTTCTGTTGCATATCTTGTTAGCATTGGTGTTACTCCTAGGGATATAGGTCCCATGGTCACCCAGTATCCTTACCTATTGGGCATGAGAGTTGGGACTGTGATTAAGCCTTTTGTTGATTATTTGGTATCCTTAGGTTTGCCGAAAAAGATTTTGGCTAGGATGTTGGAGAAGCGCGCATATTTACTTGGTTATGATCTTGAGGAGACTATTAAGCCTAATGTGGATTGTTTGGTTGCTTTTGGGATCAGGAGGGAAGCACTTGCTTCTATTGTTGCTCAATACCCCCAAATTCTTGGTCAGCCTTTGAAAGCTAAAATGTCTTCACAGCAGTTTTTCTTCAGTATGAAGCTTAAAATTGATCCTGAAGGGTTTGCACGGGTGATAGAGAAGATGCCGCAGATAGTCAGCCTTCATCAACATGTGATAATGAAGCCTGTTGAGTTCCTTCTTGGACGGGGGATACCTTCAGAAGATGTAGCTAAGATGGTCGTAAAGTGTCCCCAATTAGTGGCACTGCGTGTTGAGCTCATGAAGAACAGTTATTACTTTTTTAAGAGTGAAATGGGGAGACCAGTGAAAGAGCTTGTGGAATTTCCGGAATACTTTACATATAGTTTAGAATCCAGGATCAAACCTAGATACCAGAGGTTGCAAAGCAAGGGGATTAGGTGTTCTTTGAAATGGTTCCTCAACTGTAGCGATCAGAGATTTGAGGAGAGGTTGCAAGGGGAGTACATTGAAACAGAGATTTCTGGTCCATCATTTTGTATGGGAGGGAAGTTAGAGCTACCTGGGGTTGGAAATGAGATGGTGTCAGATGGGGAAGACGAGAGTGATGATGAAATACTTTACAGACGCACTGTATCCCTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
mTERF

Protein Analysis

515

Amino Acids

58.45

Weight (kDa)

8.62

Isoelectric Point (pI)

40.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTERF PF02536 155 - 463 1e-112 mTERF
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014188)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44020
fragaria_vesca FvH4_1g27350 FvH4_1g27350 FvH4_1g27350 FvH4_1g27350
malus_domestica MD07G1031000.v1.1
prunus_persica Prupe.8G049300_v2.0.a1
pyrus_communis pycom07g02260
rosa_chinensis RchiOBHm_Chr3g0488701
rosa_laevigata RLG00000022930
rosa_multiflora Rmu_sc0001208.1_g000078
rosa_samantha Rh3AG289100 Rh3BG325900 Rh3CG323400 Rh3DG321900
rosa_wichuraiana Rw0G013690 Rw3G025590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1183
AccII CGCG 1 cut(s) 849
AccIII TCCGGA 1 cut(s) 1270
AciI CCGC 2 cut(s) 247, 1070
AclWI GGATC 3 cut(s) 926, 1028, 1310
AcsI RAATTY 3 cut(s) 509, 963, 1265
AcuI CTGAAG 3 cut(s) 997, 1059, 1125
AdeI CACNNNGTG 1 cut(s) 1193
AfaI GTAC 3 cut(s) 416, 673, 1409
AfiI CCNNNNNNNGG 4 cut(s) 156, 157, 1183, 1472
AflIII ACRYGT 1 cut(s) 1093
AgsI TTSAA 4 cut(s) 292, 982, 1354, 1415
AjnI CCWGG 2 cut(s) 1298, 1465
AjuI GAANNNNNNNTTGG 2 cut(s) 809, 841
AloI GAACNNNNNNTCC 2 cut(s) 1330, 1362
Alw21I GWGCWC 1 cut(s) 1203
Alw26I GTCTC 3 cut(s) 268, 872, 1237
AlwI GGATC 3 cut(s) 926, 1028, 1310
Ama87I CYCGRG 1 cut(s) 203
Aor13HI TCCGGA 1 cut(s) 1270
AoxI GGCC 1 cut(s) 244
ApeKI GCWGC 1 cut(s) 13
ApoI RAATTY 3 cut(s) 509, 963, 1265
ArsI GACNNNNNNTTYG 2 cut(s) 955, 987
Asp700I GAANNNNTTC 1 cut(s) 1358
AspA2I CCTAGG 1 cut(s) 710
AspLEI GCGC 2 cut(s) 849, 851
AspS9I GGNCC 3 cut(s) 238, 722, 1430
AsuHPI GGTGA 5 cut(s) 34, 518, 580, 724, 1066
AvaI CYCGRG 1 cut(s) 203
AvaII GGWCC 3 cut(s) 238, 722, 1430
AvrII CCTAGG 1 cut(s) 710
AxyI CCTNAGG 1 cut(s) 805
BaeGI GKGCMC 1 cut(s) 130
BaeI ACNNNNGTAYC 2 cut(s) 446, 479
BanII GRGCYC 2 cut(s) 591, 1203
BbsI GAAGAC 3 cut(s) 52, 987, 1506
Bbv12I GWGCWC 1 cut(s) 1203
BbvI GCAGC 1 cut(s) 25
BccI CCATC 5 cut(s) 47, 1153, 1441, 1477, 1487
BciT130I CCWGG 2 cut(s) 1300, 1467
BciVI GTATCC 4 cut(s) 642, 750, 811, 1128
BclI TGATCA 1 cut(s) 570
BcoDI GTCTC 3 cut(s) 268, 872, 1237
BfaI CTAG 7 cut(s) 267, 386, 503, 543, 711, 831, 1311
BfmI CTRYAG 3 cut(s) 377, 1369, 1544
BfuI GTATCC 4 cut(s) 642, 750, 811, 1128
BisI GCNGC 3 cut(s) 14, 247, 1070
BlnI CCTAGG 1 cut(s) 710
BlsI GCNGC 3 cut(s) 15, 248, 1071
BmcAI AGTACT 1 cut(s) 673
Bme1390I CCNGG 2 cut(s) 1300, 1467
Bme18I GGWCC 3 cut(s) 238, 722, 1430
BmeT110I CYCGRG 1 cut(s) 203
BmgT120I GGNCC 3 cut(s) 238, 722, 1430
BmiI GGNNCC 2 cut(s) 724, 1361
BmrFI CCNGG 2 cut(s) 1300, 1467
BmrI ACTGGG 1 cut(s) 730
BmsI GCATC 3 cut(s) 414, 422, 1056
BmuI ACTGGG 1 cut(s) 730
BpiI GAAGAC 3 cut(s) 52, 987, 1506
BplI GAGNNNNNCTC 2 cut(s) 323, 355
BpuEI CTTGAG 2 cut(s) 677, 893
BsaI GGTCTC 1 cut(s) 1237
BsaJI CCNNGG 4 cut(s) 241, 710, 726, 1466
BsaWI WCCGGW 2 cut(s) 565, 1270
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 157, 1183, 1472
Bse1I ACTGG 2 cut(s) 736, 1247
Bse21I CCTNAGG 1 cut(s) 805
BseAI TCCGGA 1 cut(s) 1270
BseBI CCWGG 2 cut(s) 1300, 1467
BseDI CCNNGG 4 cut(s) 241, 710, 726, 1466
BseGI GGATG 2 cut(s) 437, 840
BseLI CCNNNNNNNGG 4 cut(s) 156, 157, 1183, 1472
BseMII CTCAG 1 cut(s) 234
BseNI ACTGG 2 cut(s) 736, 1247
BsePI GCGCGC 1 cut(s) 847
BseRI GAGGAG 2 cut(s) 890, 1403
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 1 cut(s) 25
Bsh1236I CGCG 1 cut(s) 849
BshFI GGCC 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 1203
BsiHKCI CYCGRG 1 cut(s) 203
BsiSI CCGG 2 cut(s) 566, 1271
BslFI GGGAC 4 cut(s) 676, 708, 781, 1158
BslI CCNNNNNNNGG 4 cut(s) 156, 157, 1183, 1472
BsmAI GTCTC 3 cut(s) 268, 872, 1237
BsmFI GGGAC 4 cut(s) 676, 708, 781, 1158
BsnI GGCC 1 cut(s) 246
Bso31I GGTCTC 1 cut(s) 1237
BsoBI CYCGRG 1 cut(s) 203
Bsp1286I GDGCHC 3 cut(s) 130, 591, 1203
Bsp13I TCCGGA 1 cut(s) 1270
Bsp143I GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
Bsp19I CCATGG 1 cut(s) 726
BspACI CCGC 2 cut(s) 247, 1070
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 1 cut(s) 233
BspEI TCCGGA 1 cut(s) 1270
BspFNI CGCG 1 cut(s) 849
BspHI TCATGA 2 cut(s) 354, 1203
BspLI GGNNCC 2 cut(s) 724, 1361
BspPI GGATC 3 cut(s) 926, 1028, 1310
BspTNI GGTCTC 1 cut(s) 1237
BsrI ACTGG 2 cut(s) 736, 1247
BssECI CCNNGG 4 cut(s) 241, 710, 726, 1466
BssHII GCGCGC 1 cut(s) 847
BssMI GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
BssT1I CCWWGG 3 cut(s) 241, 710, 726
Bst2UI CCWGG 2 cut(s) 1300, 1467
Bst4CI ACNGT 5 cut(s) 346, 772, 1214, 1370, 1537
Bst6I CTCTTC 2 cut(s) 19, 287
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 5 cut(s) 17, 195, 220, 805, 1155
BstDSI CCRYGG 1 cut(s) 726
BstEII GGTNACC 1 cut(s) 730
BstF5I GGATG 2 cut(s) 437, 840
BstFNI CGCG 1 cut(s) 849
BstHHI GCGC 2 cut(s) 849, 851
BstKTI GATC 6 cut(s) 573, 871, 921, 1036, 1305, 1378
BstMAI GTCTC 3 cut(s) 268, 872, 1237
BstMBI GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
BstMWI GCNNNNNNNGC 1 cut(s) 1198
BstNI CCWGG 2 cut(s) 1300, 1467
BstNSI RCATGY 2 cut(s) 542, 1097
BstPI GGTNACC 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 1298, 1465
BstSFI CTRYAG 3 cut(s) 377, 1369, 1544
BstSLI GKGCMC 1 cut(s) 130
BstUI CGCG 1 cut(s) 849
BstV1I GCAGC 1 cut(s) 25
BstV2I GAAGAC 3 cut(s) 52, 987, 1506
BstXI CCANNNNNNTGG 1 cut(s) 968
Bsu36I CCTNAGG 1 cut(s) 805
BsuI GTATCC 4 cut(s) 642, 750, 811, 1128
BsuRI GGCC 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 726
BtsCI GGATG 2 cut(s) 437, 840
BtsI GCAGTG 2 cut(s) 442, 1187
BtsIMutI CAGTG 4 cut(s) 442, 1187, 1254, 1533
Cac8I GCNNGC 1 cut(s) 849
CciI TCATGA 2 cut(s) 354, 1203
CfoI GCGC 2 cut(s) 849, 851
Cfr13I GGNCC 3 cut(s) 238, 722, 1430
CseI GACGC 1 cut(s) 1539
Csp6I GTAC 3 cut(s) 415, 672, 1408
CviAII CATG 8 cut(s) 123, 355, 451, 539, 727, 757, 1094, 1204
CviQI GTAC 3 cut(s) 415, 672, 1408
DdeI CTNAG 5 cut(s) 17, 195, 220, 805, 1155
DpnI GATC 6 cut(s) 572, 870, 920, 1035, 1304, 1377
DpnII GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
DraIII CACNNNGTG 1 cut(s) 1193
Eam1104I CTCTTC 2 cut(s) 19, 287
EarI CTCTTC 2 cut(s) 19, 287
Ecl136II GAGCTC 1 cut(s) 1201
Eco130I CCWWGG 3 cut(s) 241, 710, 726
Eco24I GRGCYC 2 cut(s) 591, 1203
Eco31I GGTCTC 1 cut(s) 1237
Eco47I GGWCC 3 cut(s) 238, 722, 1430
Eco53kI GAGCTC 1 cut(s) 1201
Eco57I CTGAAG 3 cut(s) 997, 1059, 1125
Eco81I CCTNAGG 1 cut(s) 805
Eco88I CYCGRG 1 cut(s) 203
Eco91I GGTNACC 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 1201
EcoO109I RGGNCCY 1 cut(s) 722
EcoO65I GGTNACC 1 cut(s) 730
EcoRII CCWGG 2 cut(s) 1298, 1465
EcoT14I CCWWGG 3 cut(s) 241, 710, 726
EcoT38I GRGCYC 2 cut(s) 591, 1203
ErhI CCWWGG 3 cut(s) 241, 710, 726
FaeI CATG 8 cut(s) 126, 358, 454, 542, 730, 760, 1097, 1207
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 4 cut(s) 676, 708, 781, 1158
FatI CATG 8 cut(s) 122, 354, 450, 538, 726, 756, 1093, 1203
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 3 cut(s) 14, 247, 1070
FokI GGATG 2 cut(s) 444, 847
FriOI GRGCYC 2 cut(s) 591, 1203
Fsp4HI GCNGC 3 cut(s) 14, 247, 1070
FspBI CTAG 7 cut(s) 267, 386, 503, 543, 711, 831, 1311
GlaI GCGC 2 cut(s) 848, 850
GluI GCNGC 3 cut(s) 14, 247, 1070
HaeIII GGCC 1 cut(s) 246
HapII CCGG 2 cut(s) 566, 1271
HgaI GACGC 1 cut(s) 1539
HhaI GCGC 2 cut(s) 849, 851
Hin1II CATG 8 cut(s) 126, 358, 454, 542, 730, 760, 1097, 1207
Hin6I GCGC 2 cut(s) 847, 849
HinP1I GCGC 2 cut(s) 847, 849
HindIII AAGCTT 3 cut(s) 257, 653, 1022
HinfI GANTC 3 cut(s) 270, 487, 1295
HpaII CCGG 2 cut(s) 566, 1271
HphI GGTGA 5 cut(s) 34, 518, 580, 724, 1066
Hpy188I TCNGA 6 cut(s) 431, 492, 647, 1144, 1380, 1492
HpyAV CCTTC 5 cut(s) 590, 1034, 1094, 1130, 1149
HpyCH4III ACNGT 5 cut(s) 346, 772, 1214, 1370, 1537
HpyCH4V TGCA 6 cut(s) 435, 625, 683, 1049, 1327, 1399
HpyF10VI GCNNNNNNNGC 1 cut(s) 1198
HpyF3I CTNAG 5 cut(s) 17, 195, 220, 805, 1155
Hsp92II CATG 8 cut(s) 126, 358, 454, 542, 730, 760, 1097, 1207
HspAI GCGC 2 cut(s) 847, 849
Kpn2I TCCGGA 1 cut(s) 1270
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
LmnI GCTCC 2 cut(s) 91, 331
Lsp1109I GCAGC 1 cut(s) 25
LweI GCATC 3 cut(s) 414, 422, 1056
MaeI CTAG 7 cut(s) 267, 386, 503, 543, 711, 831, 1311
MaeIII GTNAC 6 cut(s) 173, 223, 281, 534, 703, 730
MalI GATC 6 cut(s) 572, 870, 920, 1035, 1304, 1377
MboI GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
MboII GAAGA 9 cut(s) 36, 52, 304, 987, 1003, 1075, 1157, 1219, 1511
MhlI GDGCHC 3 cut(s) 130, 591, 1203
MluCI AATT 8 cut(s) 317, 480, 509, 520, 963, 1029, 1178, 1265
MlyI GAGTC 1 cut(s) 279
MmeI TCCRAC 2 cut(s) 819, 1453
MroI TCCGGA 1 cut(s) 1270
MroXI GAANNNNTTC 1 cut(s) 1358
MseI TTAA 6 cut(s) 408, 516, 777, 885, 1026, 1227
MslI CAYNNNNRTG 2 cut(s) 543, 1092
MspI CCGG 2 cut(s) 566, 1271
MspR9I CCNGG 2 cut(s) 1300, 1467
MvaI CCWGG 2 cut(s) 1300, 1467
MvnI CGCG 1 cut(s) 849
MwoI GCNNNNNNNGC 1 cut(s) 1198
NcoI CCATGG 1 cut(s) 726
NdeII GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
NlaIII CATG 8 cut(s) 126, 358, 454, 542, 730, 760, 1097, 1207
NlaIV GGNNCC 2 cut(s) 724, 1361
NmuCI GTSAC 4 cut(s) 173, 223, 281, 730
NspI RCATGY 2 cut(s) 542, 1097
PagI TCATGA 2 cut(s) 354, 1203
PauI GCGCGC 1 cut(s) 847
PciI ACATGT 1 cut(s) 1093
PcsI WCGNNNNNNNCGW 1 cut(s) 408
PdmI GAANNNNTTC 1 cut(s) 1358
PfeI GAWTC 2 cut(s) 487, 1295
PflMI CCANNNNNTGG 1 cut(s) 1183
PfoI TCCNGGA 1 cut(s) 1298
PkrI GCNGC 3 cut(s) 15, 248, 1071
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
PpuMI RGGWCCY 1 cut(s) 722
PscI ACATGT 1 cut(s) 1093
Psp124BI GAGCTC 1 cut(s) 1203
Psp5II RGGWCCY 1 cut(s) 722
Psp6I CCWGG 2 cut(s) 1298, 1465
PspEI GGTNACC 1 cut(s) 730
PspGI CCWGG 2 cut(s) 1298, 1465
PspN4I GGNNCC 2 cut(s) 724, 1361
PspPI GGNCC 3 cut(s) 238, 722, 1430
PspPPI RGGWCCY 1 cut(s) 722
PsrI GAACNNNNNNTAC 2 cut(s) 1202, 1234
PteI GCGCGC 1 cut(s) 847
RsaI GTAC 3 cut(s) 416, 673, 1409
RsaNI GTAC 3 cut(s) 415, 672, 1408
RseI CAYNNNNRTG 2 cut(s) 543, 1092
SacI GAGCTC 1 cut(s) 1203
SaqAI TTAA 6 cut(s) 408, 516, 777, 885, 1026, 1227
SatI GCNGC 3 cut(s) 14, 247, 1070
Sau3AI GATC 6 cut(s) 570, 868, 918, 1033, 1302, 1375
Sau96I GGNCC 3 cut(s) 238, 722, 1430
ScaI AGTACT 1 cut(s) 673
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 2 cut(s) 1300, 1467
SduI GDGCHC 3 cut(s) 130, 591, 1203
SfaNI GCATC 3 cut(s) 414, 422, 1056
SfcI CTRYAG 3 cut(s) 377, 1369, 1544
SinI GGWCC 3 cut(s) 238, 722, 1430
SmiMI CAYNNNNRTG 2 cut(s) 543, 1092
SmlI CTYRAG 2 cut(s) 656, 872
SmoI CTYRAG 2 cut(s) 656, 872
Sse9I AATT 8 cut(s) 317, 480, 509, 520, 963, 1029, 1178, 1265
SsiI CCGC 2 cut(s) 247, 1070
SspMI CTAG 7 cut(s) 267, 386, 503, 543, 711, 831, 1311
SstI GAGCTC 1 cut(s) 1203
StyD4I CCNGG 2 cut(s) 1298, 1465
StyI CCWWGG 3 cut(s) 241, 710, 726
TaaI ACNGT 5 cut(s) 346, 772, 1214, 1370, 1537
TaqI TCGA 3 cut(s) 395, 497, 591
TasI AATT 8 cut(s) 317, 480, 509, 520, 963, 1029, 1178, 1265
TatI WGTACW 2 cut(s) 671, 1407
TauI GCSGC 2 cut(s) 249, 1072
TfiI GAWTC 2 cut(s) 487, 1295
Tru1I TTAA 6 cut(s) 408, 516, 777, 885, 1026, 1227
Tru9I TTAA 6 cut(s) 408, 516, 777, 885, 1026, 1227
TscAI CASTG 4 cut(s) 442, 1194, 1254, 1540
TseFI GTSAC 4 cut(s) 173, 223, 281, 730
TseI GCWGC 1 cut(s) 13
Tsp45I GTSAC 4 cut(s) 173, 223, 281, 730
TspDTI ATGAA 7 cut(s) 225, 330, 1034, 1076, 1118, 1220, 1530
TspRI CASTG 4 cut(s) 442, 1194, 1254, 1540
Van91I CCANNNNNTGG 1 cut(s) 1183
VpaK11BI GGWCC 3 cut(s) 238, 722, 1430
XapI RAATTY 3 cut(s) 509, 963, 1265
XbaI TCTAGA 1 cut(s) 266
XceI RCATGY 2 cut(s) 542, 1097
XmaJI CCTAGG 1 cut(s) 710
XmnI GAANNNNTTC 1 cut(s) 1358
XspI CTAG 7 cut(s) 267, 386, 503, 543, 711, 831, 1311
ZrmI AGTACT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.