RchiOBHm_Chr4g0387291

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0017001
Physical Location & Seq
Reverse (-)
2453312 .. 2458716
5405 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36061

Sequence Viewer

Length: 825 bp
ATGAACTTGTGGAATTGCCATAGATTTAGTTACAATCTGGGCTATGACATGGAGAAGATGGAAAGTATTTTGCTGAATAATCAGAACAACTCCTGGTTTAGTCTTGTATTACCGGGTAGTGGGATTCCGAAGTGGTTCCATTGTTCGAAGGAGGTTACTGCTAATGAAAAAGGCCCATCCAGTAAATGTGAAATTTCTTTTGAAATCCCAGGCAAATTGAATTGGGAGTACATAGGAGTGGCTCTTTGTTCTGTTTCTTGTTTTTCAGTTGACGAAAATTTTTATTTTTTTATCAAGGATCGAGCTGATATTTCCATCAATGAGGTACTCATAGATCAGTGTTCCAAGTTGCGCAGTTCAGCGCCAGACCACATTTGGCTAGCTTATGTTCCATTATCTGATAAAATAAAGGGAAAGGAGGATCAAAATGGTCGGTGTCAAGTTCGATTTTACTGTGCTGGAATGAAAAGCTATGGAGTTCACCTCGTATGCCAACCACCGAATGAATATTCAAATAAGATTGTAATGGCGGCTGAACCTGCCCATGAGAGTGGTATGCTAGACTGCCTTTCGTTTTCTTATGCGAATCCAGAACAAAAGAGGCCTCTTACTGAAGCCACCGACGATTATGAGTATGACCATCAACAAGAGCAGCATGAGCAACCAATAGGAATGATTCCTTCGAAACGACTGTGCACTGAGGAGCAACCATCGATGGCTCCCAACCCAATCATCCACGACTACTCCGATTACTCTGCATCTGCATCCACATCCACATCAGATATATATGCCACCGAAGAGGCCAGAACAAAAGAGGCCTCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

274

Amino Acids

31.11

Weight (kDa)

4.99

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 547
AciI CCGC 1 cut(s) 530
AclWI GGATC 2 cut(s) 306, 429
AcsI RAATTY 2 cut(s) 192, 277
AcuI CTGAAG 1 cut(s) 633
AfaI GTAC 2 cut(s) 230, 327
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 3 cut(s) 203, 220, 513
AjnI CCWGG 2 cut(s) 92, 208
AluBI AGCT 3 cut(s) 305, 383, 471
AluI AGCT 3 cut(s) 305, 383, 471
Alw21I GWGCWC 1 cut(s) 698
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 2 cut(s) 306, 429
AoxI GGCC 4 cut(s) 172, 602, 801, 816
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 1 cut(s) 652
ApoI RAATTY 2 cut(s) 192, 277
Asp700I GAANNNNTTC 1 cut(s) 134
AspLEI GCGC 2 cut(s) 354, 364
AspS9I GGNCC 1 cut(s) 173
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 1 cut(s) 473
AsuII TTCGAA 2 cut(s) 146, 683
AsuNHI GCTAGC 1 cut(s) 379
BaeGI GKGCMC 1 cut(s) 698
Bbv12I GWGCWC 1 cut(s) 698
BbvI GCAGC 1 cut(s) 664
BccI CCATC 6 cut(s) 52, 184, 323, 648, 709, 718
BcgI CGANNNNNNTGC 2 cut(s) 737, 771
BciT130I CCWGG 2 cut(s) 94, 210
BcnI CCSGG 1 cut(s) 114
BfaI CTAG 2 cut(s) 380, 560
BfoI RGCGCY 1 cut(s) 365
BfuAI ACCTGC 1 cut(s) 547
BisI GCNGC 2 cut(s) 531, 653
BlsI GCNGC 2 cut(s) 532, 654
Bme1390I CCNGG 3 cut(s) 94, 114, 210
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 2 cut(s) 137, 720
BmrFI CCNGG 3 cut(s) 94, 114, 210
BmsI GCATC 2 cut(s) 767, 773
BmtI GCTAGC 1 cut(s) 383
BplI GAGNNNNNCTC 2 cut(s) 468, 500
Bpu14I TTCGAA 2 cut(s) 146, 683
BpuMI CCSGG 1 cut(s) 114
Bsa29I ATCGAT 1 cut(s) 713
BsaJI CCNNGG 1 cut(s) 208
BsaXI ACNNNNNCTCC 2 cut(s) 728, 758
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 180
BseBI CCWGG 2 cut(s) 94, 210
BseCI ATCGAT 1 cut(s) 713
BseDI CCNNGG 1 cut(s) 208
BseGI GGATG 4 cut(s) 176, 732, 764, 770
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 1 cut(s) 690
BseNI ACTGG 1 cut(s) 180
BseRI GAGGAG 1 cut(s) 716
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 1 cut(s) 664
BshFI GGCC 4 cut(s) 174, 604, 803, 818
BshVI ATCGAT 1 cut(s) 713
BsiHKAI GWGCWC 1 cut(s) 698
BsiSI CCGG 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 119
BsnI GGCC 4 cut(s) 174, 604, 803, 818
Bsp119I TTCGAA 2 cut(s) 146, 683
Bsp1286I GDGCHC 1 cut(s) 698
Bsp143I GATC 3 cut(s) 298, 334, 421
BspACI CCGC 1 cut(s) 530
BspANI GGCC 4 cut(s) 174, 604, 803, 818
BspCNI CTCAG 1 cut(s) 691
BspDI ATCGAT 1 cut(s) 713
BspLI GGNNCC 2 cut(s) 137, 720
BspMI ACCTGC 1 cut(s) 547
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 2 cut(s) 306, 429
BspT104I TTCGAA 2 cut(s) 146, 683
BsrI ACTGG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 208
BssMI GATC 3 cut(s) 298, 334, 421
Bst2UI CCWGG 2 cut(s) 94, 210
Bst4CI ACNGT 2 cut(s) 455, 693
Bst6I CTCTTC 1 cut(s) 792
BstBI TTCGAA 2 cut(s) 146, 683
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 699
BstF5I GGATG 4 cut(s) 176, 732, 764, 770
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 2 cut(s) 354, 364
BstKTI GATC 3 cut(s) 301, 337, 424
BstMBI GATC 3 cut(s) 298, 334, 421
BstMWI GCNNNNNNNGC 2 cut(s) 539, 658
BstNI CCWGG 2 cut(s) 94, 210
BstSCI CCNGG 3 cut(s) 92, 112, 208
BstSLI GKGCMC 1 cut(s) 698
BstV1I GCAGC 1 cut(s) 664
BstXI CCANNNNNNTGG 1 cut(s) 551
Bsu15I ATCGAT 1 cut(s) 713
BsuRI GGCC 4 cut(s) 174, 604, 803, 818
BsuTUI ATCGAT 1 cut(s) 713
BtsCI GGATG 4 cut(s) 176, 732, 764, 770
BtsIMutI CAGTG 2 cut(s) 344, 696
BveI ACCTGC 1 cut(s) 547
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 2 cut(s) 354, 364
Cfr13I GGNCC 1 cut(s) 173
ClaI ATCGAT 1 cut(s) 713
Csp6I GTAC 2 cut(s) 229, 326
CviAII CATG 3 cut(s) 49, 545, 656
CviQI GTAC 2 cut(s) 229, 326
DdeI CTNAG 1 cut(s) 699
DpnI GATC 3 cut(s) 300, 336, 423
DpnII GATC 3 cut(s) 298, 334, 421
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco147I AGGCCT 2 cut(s) 604, 818
Eco57I CTGAAG 1 cut(s) 633
EcoRII CCWGG 2 cut(s) 92, 208
FaeI CATG 3 cut(s) 52, 548, 659
FatI CATG 3 cut(s) 48, 544, 655
Fnu4HI GCNGC 2 cut(s) 531, 653
FokI GGATG 4 cut(s) 163, 719, 751, 757
Fsp4HI GCNGC 2 cut(s) 531, 653
FspBI CTAG 2 cut(s) 380, 560
FspI TGCGCA 1 cut(s) 353
GlaI GCGC 2 cut(s) 353, 363
GluI GCNGC 2 cut(s) 531, 653
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 4 cut(s) 174, 604, 803, 818
HapII CCGG 1 cut(s) 113
HhaI GCGC 2 cut(s) 354, 364
Hin1II CATG 3 cut(s) 52, 548, 659
Hin6I GCGC 2 cut(s) 352, 362
HinP1I GCGC 2 cut(s) 352, 362
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HinfI GANTC 3 cut(s) 124, 586, 676
HpaII CCGG 1 cut(s) 113
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 3 cut(s) 271, 481, 696
Hpy188I TCNGA 5 cut(s) 84, 129, 400, 748, 781
Hpy188III TCNNGA 1 cut(s) 590
Hpy8I GTNNAC 3 cut(s) 271, 481, 696
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 2 cut(s) 142, 690
HpyCH4III ACNGT 2 cut(s) 455, 693
HpyCH4V TGCA 3 cut(s) 696, 758, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 658
HpyF3I CTNAG 1 cut(s) 699
Hsp92II CATG 3 cut(s) 52, 548, 659
HspAI GCGC 2 cut(s) 352, 362
Kzo9I GATC 3 cut(s) 298, 334, 421
LmnI GCTCC 2 cut(s) 703, 724
Lsp1109I GCAGC 1 cut(s) 664
LweI GCATC 2 cut(s) 767, 773
MaeI CTAG 2 cut(s) 380, 560
MaeIII GTNAC 2 cut(s) 29, 154
MalI GATC 3 cut(s) 300, 336, 423
MboI GATC 3 cut(s) 298, 334, 421
MboII GAAGA 2 cut(s) 67, 809
MhlI GDGCHC 1 cut(s) 698
MluCI AATT 5 cut(s) 13, 192, 215, 220, 277
MnlI CCTC 9 cut(s) 145, 316, 412, 494, 594, 615, 694, 793, 808
MroXI GAANNNNTTC 1 cut(s) 134
MslI CAYNNNNRTG 2 cut(s) 236, 549
MspI CCGG 1 cut(s) 113
MspR9I CCNGG 3 cut(s) 94, 114, 210
MvaI CCWGG 2 cut(s) 94, 210
MwoI GCNNNNNNNGC 2 cut(s) 539, 658
NciI CCSGG 1 cut(s) 114
NdeII GATC 3 cut(s) 298, 334, 421
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 3 cut(s) 52, 548, 659
NlaIV GGNNCC 2 cut(s) 137, 720
NsbI TGCGCA 1 cut(s) 353
NspV TTCGAA 2 cut(s) 146, 683
PceI AGGCCT 2 cut(s) 604, 818
PcsI WCGNNNNNNNCGW 1 cut(s) 744
PdmI GAANNNNTTC 1 cut(s) 134
PfeI GAWTC 3 cut(s) 124, 586, 676
PkrI GCNGC 2 cut(s) 532, 654
Psp6I CCWGG 2 cut(s) 92, 208
PspGI CCWGG 2 cut(s) 92, 208
PspN4I GGNNCC 2 cut(s) 137, 720
PspPI GGNCC 1 cut(s) 173
RsaI GTAC 2 cut(s) 230, 327
RsaNI GTAC 2 cut(s) 229, 326
RseI CAYNNNNRTG 2 cut(s) 236, 549
SatI GCNGC 2 cut(s) 531, 653
Sau3AI GATC 3 cut(s) 298, 334, 421
Sau96I GGNCC 1 cut(s) 173
ScrFI CCNGG 3 cut(s) 94, 114, 210
SduI GDGCHC 1 cut(s) 698
SetI ASST 7 cut(s) 156, 307, 327, 385, 473, 486, 541
SfaNI GCATC 2 cut(s) 767, 773
SfuI TTCGAA 2 cut(s) 146, 683
SmiMI CAYNNNNRTG 2 cut(s) 236, 549
Sse9I AATT 5 cut(s) 13, 192, 215, 220, 277
SseBI AGGCCT 2 cut(s) 604, 818
SsiI CCGC 1 cut(s) 530
SspI AATATT 1 cut(s) 509
SspMI CTAG 2 cut(s) 380, 560
StuI AGGCCT 2 cut(s) 604, 818
StyD4I CCNGG 3 cut(s) 92, 112, 208
TaaI ACNGT 2 cut(s) 455, 693
TaqI TCGA 5 cut(s) 146, 301, 445, 683, 713
TasI AATT 5 cut(s) 13, 192, 215, 220, 277
TatI WGTACW 1 cut(s) 228
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 3 cut(s) 124, 586, 676
TscAI CASTG 2 cut(s) 344, 703
TseI GCWGC 1 cut(s) 652
TspDTI ATGAA 4 cut(s) 17, 180, 479, 519
TspRI CASTG 2 cut(s) 344, 703
VneI GTGCAC 1 cut(s) 694
XapI RAATTY 2 cut(s) 192, 277
XcmI CCANNNNNNNNNTGG 1 cut(s) 372
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 380, 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.