RchiOBHm_Chr4g0388831

Vegetative cell wall protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018529
Physical Location & Seq
Reverse (-)
4015234 .. 4015902
669 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36200

Sequence Viewer

Length: 585 bp
ATGCAAAAGGTTACAATAATGTCGTCCATCTCTGCAATCTCATTCACACTCATAAAACGAACCATTCCACTTTGCTCAGTATCCAAAACAAGTACTAGCACCAGTACCCCTCCATTGTTATTGTCACTCAAGAACCCTATCTTTCCCACTCAACCTCAGATTTTAGTCCATGCTCGATTATCTGGCTCACCCAACGAGTATCCAAATCGGAGCCCGCCGGAGTTTTCTCCTCCGGTGCCAGGTTTTGACCGGCCTTCTGTTCCACCCGAAGTACCTGGAATATCTACCTCTCCGGAAGTTGACATCCCAACAACTACTCCAGTAACTCCACCTGAGGTCAGCCCTTATCCTCCACCACTTGACCCCGGTCCGAACCCGGGTCCGGATTTTCCAGTACCTCCTTTGAAGCCACCTCCTGTTCCGGATGTGCCACGTCCTTTTCCAGACAACCCAATTCCAGGACCTGACAGACTGCCGCCTCAGCCACCGGATATAGTGCCTCCACCGCCTCCTGGGCCTGAGATTATTCCACCGCCAGCACCGCCGCCACCGCCTACCGGTCCAGGCGGGCCTATTGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

194

Amino Acids

20.29

Weight (kDa)

5.01

Isoelectric Point (pI)

73.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018529)

Species Orthologous Gene IDs
prunus_persica Prupe.1G023900_v2.0.a1
pyrus_communis pycom13g17550
rosa_chinensis RchiOBHm_Chr4g0388831
rosa_laevigata RLG00000010022
rosa_multiflora Rmu_sc0000697.1_g000012 Rmu_sc0016023.1_g000003
rosa_rugosa Rorug03G0320200
rosa_samantha Rh4BG018000 Rh4CG027700 Rh4DG018600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccIII TCCGGA 3 cut(s) 292, 382, 421
AciI CCGC 8 cut(s) 215, 476, 506, 533, 542, 545, 551, 567
AfaI GTAC 4 cut(s) 94, 106, 273, 396
AfiI CCNNNNNNNGG 6 cut(s) 239, 377, 382, 458, 512, 557
AgeI ACCGGT 1 cut(s) 557
AgsI TTSAA 1 cut(s) 406
AjiI CACGTC 1 cut(s) 434
AjnI CCWGG 5 cut(s) 238, 274, 457, 511, 562
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 376
Aor13HI TCCGGA 3 cut(s) 292, 382, 421
AoxI GGCC 3 cut(s) 251, 515, 569
AsiGI ACCGGT 1 cut(s) 557
AspS9I GGNCC 6 cut(s) 368, 380, 461, 515, 560, 569
AsuC2I CCSGG 3 cut(s) 366, 377, 378
AsuHPI GGTGA 1 cut(s) 180
AvaI CYCGRG 1 cut(s) 376
AvaII GGWCC 4 cut(s) 368, 380, 461, 560
AxyI CCTNAGG 1 cut(s) 333
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 215
BbvCI CCTCAGC 1 cut(s) 480
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 5 cut(s) 240, 276, 459, 513, 564
BciVI GTATCC 2 cut(s) 91, 210
BcnI CCSGG 3 cut(s) 366, 377, 378
BfaI CTAG 1 cut(s) 96
BfuI GTATCC 2 cut(s) 91, 210
BglI GCCNNNNNGGC 1 cut(s) 514
BisI GCNGC 2 cut(s) 476, 545
BlsI GCNGC 2 cut(s) 477, 546
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 8 cut(s) 240, 276, 366, 377, 378, 459, 513, 564
Bme18I GGWCC 4 cut(s) 368, 380, 461, 560
BmeT110I CYCGRG 1 cut(s) 376
BmgBI CACGTC 1 cut(s) 434
BmgT120I GGNCC 6 cut(s) 368, 380, 461, 515, 560, 569
BmiI GGNNCC 3 cut(s) 212, 237, 381
BmrFI CCNGG 8 cut(s) 240, 276, 366, 377, 378, 459, 513, 564
BoxI GACNNNNGTC 1 cut(s) 366
BpmI CTGGAG 1 cut(s) 303
Bpu10I CCTNAGC 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 3 cut(s) 366, 377, 378
BsaJI CCNNGG 3 cut(s) 364, 376, 512
BsaWI WCCGGW 6 cut(s) 232, 292, 382, 421, 487, 557
BsaXI ACNNNNNCTCC 2 cut(s) 301, 331
Bsc4I CCNNNNNNNGG 6 cut(s) 239, 377, 382, 458, 512, 557
Bse118I RCCGGY 2 cut(s) 249, 557
Bse1I ACTGG 3 cut(s) 102, 320, 392
Bse21I CCTNAGG 1 cut(s) 333
BseAI TCCGGA 3 cut(s) 292, 382, 421
BseBI CCWGG 5 cut(s) 240, 276, 459, 513, 564
BseDI CCNNGG 3 cut(s) 364, 376, 512
BseGI GGATG 2 cut(s) 303, 430
BseLI CCNNNNNNNGG 6 cut(s) 239, 377, 382, 458, 512, 557
BseMII CTCAG 5 cut(s) 90, 170, 324, 494, 510
BseNI ACTGG 3 cut(s) 102, 320, 392
BseRI GAGGAG 1 cut(s) 219
BshFI GGCC 3 cut(s) 253, 517, 571
BshNI GGYRCC 1 cut(s) 235
BshTI ACCGGT 1 cut(s) 557
BsiHKCI CYCGRG 1 cut(s) 376
BslI CCNNNNNNNGG 6 cut(s) 239, 377, 382, 458, 512, 557
BsnI GGCC 3 cut(s) 253, 517, 571
BsoBI CYCGRG 1 cut(s) 376
Bsp1286I GDGCHC 1 cut(s) 215
Bsp13I TCCGGA 3 cut(s) 292, 382, 421
BspACI CCGC 8 cut(s) 215, 476, 506, 533, 542, 545, 551, 567
BspANI GGCC 3 cut(s) 253, 517, 571
BspCNI CTCAG 5 cut(s) 89, 169, 325, 493, 511
BspEI TCCGGA 3 cut(s) 292, 382, 421
BspLI GGNNCC 3 cut(s) 212, 237, 381
BspT107I GGYRCC 1 cut(s) 235
BsrFI RCCGGY 2 cut(s) 249, 557
BsrI ACTGG 3 cut(s) 102, 320, 392
BssAI RCCGGY 2 cut(s) 249, 557
BssECI CCNNGG 3 cut(s) 364, 376, 512
Bst2UI CCWGG 5 cut(s) 240, 276, 459, 513, 564
BstC8I GCNNGC 3 cut(s) 215, 537, 569
BstDEI CTNAG 5 cut(s) 76, 156, 333, 480, 519
BstF5I GGATG 2 cut(s) 303, 430
BstMWI GCNNNNNNNGC 5 cut(s) 481, 505, 514, 541, 550
BstNI CCWGG 5 cut(s) 240, 276, 459, 513, 564
BstPAI GACNNNNGTC 1 cut(s) 366
BstSCI CCNGG 8 cut(s) 238, 274, 364, 375, 376, 457, 511, 562
Bsu36I CCTNAGG 1 cut(s) 333
BsuI GTATCC 2 cut(s) 91, 210
BsuRI GGCC 3 cut(s) 253, 517, 571
BtrI CACGTC 1 cut(s) 434
BtsCI GGATG 2 cut(s) 303, 430
Cac8I GCNNGC 3 cut(s) 215, 537, 569
CaiI CAGNNNCTG 1 cut(s) 464
Cfr10I RCCGGY 2 cut(s) 249, 557
Cfr13I GGNCC 6 cut(s) 368, 380, 461, 515, 560, 569
Cfr9I CCCGGG 1 cut(s) 376
CpoI CGGWCCG 1 cut(s) 368
Csp6I GTAC 4 cut(s) 93, 105, 272, 395
CspAI ACCGGT 1 cut(s) 557
CspI CGGWCCG 1 cut(s) 368
CviAII CATG 1 cut(s) 170
CviJI RGCY 8 cut(s) 186, 213, 253, 342, 409, 484, 517, 571
CviKI_1 RGCY 8 cut(s) 186, 213, 253, 342, 409, 484, 517, 571
CviQI GTAC 4 cut(s) 93, 105, 272, 395
DdeI CTNAG 5 cut(s) 76, 156, 333, 480, 519
Eco24I GRGCYC 1 cut(s) 215
Eco47I GGWCC 4 cut(s) 368, 380, 461, 560
Eco81I CCTNAGG 1 cut(s) 333
Eco88I CYCGRG 1 cut(s) 376
EcoO109I RGGNCCY 1 cut(s) 461
EcoRII CCWGG 5 cut(s) 238, 274, 457, 511, 562
EcoT38I GRGCYC 1 cut(s) 215
FaeI CATG 1 cut(s) 173
FaiI YATR 3 cut(s) 53, 171, 494
FatI CATG 1 cut(s) 169
FauI CCCGC 2 cut(s) 222, 560
Fnu4HI GCNGC 2 cut(s) 476, 545
FokI GGATG 2 cut(s) 290, 437
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 476, 545
FspBI CTAG 1 cut(s) 96
GluI GCNGC 2 cut(s) 476, 545
GsuI CTGGAG 1 cut(s) 303
HaeIII GGCC 3 cut(s) 253, 517, 571
Hin1II CATG 1 cut(s) 173
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 159, 210, 372
Hpy188III TCNNGA 5 cut(s) 130, 293, 383, 422, 443
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 264
HpyCH4IV ACGT 1 cut(s) 433
HpyCH4V TGCA 2 cut(s) 4, 35
HpyF10VI GCNNNNNNNGC 5 cut(s) 481, 505, 514, 541, 550
HpyF3I CTNAG 5 cut(s) 76, 156, 333, 480, 519
HpySE526I ACGT 1 cut(s) 433
Hsp92II CATG 1 cut(s) 173
Kpn2I TCCGGA 3 cut(s) 292, 382, 421
LmnI GCTCC 1 cut(s) 210
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 433
MaeIII GTNAC 3 cut(s) 10, 123, 322
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 1 cut(s) 453
MroI TCCGGA 3 cut(s) 292, 382, 421
MspR9I CCNGG 8 cut(s) 240, 276, 366, 377, 378, 459, 513, 564
MvaI CCWGG 5 cut(s) 240, 276, 459, 513, 564
MwoI GCNNNNNNNGC 5 cut(s) 481, 505, 514, 541, 550
NciI CCSGG 3 cut(s) 366, 377, 378
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 3 cut(s) 212, 237, 381
NmuCI GTSAC 1 cut(s) 123
PfoI TCCNGGA 1 cut(s) 457
PinAI ACCGGT 1 cut(s) 557
PkrI GCNGC 2 cut(s) 477, 546
PpuMI RGGWCCY 1 cut(s) 461
PshAI GACNNNNGTC 1 cut(s) 366
Psp5II RGGWCCY 1 cut(s) 461
Psp6I CCWGG 5 cut(s) 238, 274, 457, 511, 562
PspGI CCWGG 5 cut(s) 238, 274, 457, 511, 562
PspN4I GGNNCC 3 cut(s) 212, 237, 381
PspPI GGNCC 6 cut(s) 368, 380, 461, 515, 560, 569
PspPPI RGGWCCY 1 cut(s) 461
PstNI CAGNNNCTG 1 cut(s) 464
RsaI GTAC 4 cut(s) 94, 106, 273, 396
RsaNI GTAC 4 cut(s) 93, 105, 272, 395
Rsr2I CGGWCCG 1 cut(s) 368
RsrII CGGWCCG 1 cut(s) 368
SatI GCNGC 2 cut(s) 476, 545
Sau96I GGNCC 6 cut(s) 368, 380, 461, 515, 560, 569
ScaI AGTACT 1 cut(s) 94
ScrFI CCNGG 8 cut(s) 240, 276, 366, 377, 378, 459, 513, 564
SduI GDGCHC 1 cut(s) 215
SinI GGWCC 4 cut(s) 368, 380, 461, 560
SmaI CCCGGG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 453
SsiI CCGC 8 cut(s) 215, 476, 506, 533, 542, 545, 551, 567
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 8 cut(s) 238, 274, 364, 375, 376, 457, 511, 562
TaiI ACGT 1 cut(s) 436
TaqI TCGA 1 cut(s) 175
TasI AATT 1 cut(s) 453
TatI WGTACW 1 cut(s) 92
TauI GCSGC 2 cut(s) 478, 547
TseFI GTSAC 1 cut(s) 123
Tsp45I GTSAC 1 cut(s) 123
TspMI CCCGGG 1 cut(s) 376
VpaK11BI GGWCC 4 cut(s) 368, 380, 461, 560
XmaI CCCGGG 1 cut(s) 376
XspI CTAG 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.