RLG00000010022

Vegetative cell wall protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0018529
Physical Location & Seq
Forward (+)
61835861 .. 61836418
558 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010022

Sequence Viewer

Length: 558 bp
ATGTCGTCCATCTCTGCAATCTCATTCACATTCGTAAAACGAACCATTCCACTTTGCTCAGTATCCAAAACAAGTACTAGCACCAGTACCCCTCCATTGTTATTGTCACCCAAGAACCCTATCTTTCCCACTCGACCTCAGGTTTTAGTCCATGCTCGATTATCTGACTCACCCCACGAGTATCCAACTCGGAGCCAGCCGGAGTTTTCTCCTCCGCTGCCGGGTTTTGACCGGCCTTCTGTTCCACCTGAAGTACCTGGAATATCTACCTCTCCGGAAGTTGATATCACAACTACTACTCCACCTGAAGTCAGCCCTTATCCTCCACCACTTGACCCGGATCCGAACCCGGGTCCAGATTTTCCAGTACCTCCTTTGAAGCCACCTCCTGTTCCGGATGTGCCACGTCCTTTTCCAGATAACCCGATTCCAGGACCTGACAGACTGCCGCCGCAGCCACCGGATATAGTGCCTCCACCGCCTCCTGGGCCTGAGATTATTCCACCGCCAGCACCGCAGCCACCCCCTACCGACCCAGGCGGGCCTAGTGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

186

Amino Acids

19.55

Weight (kDa)

4.72

Isoelectric Point (pI)

80.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018529)

Species Orthologous Gene IDs
prunus_persica Prupe.1G023900_v2.0.a1
pyrus_communis pycom13g17550
rosa_chinensis RchiOBHm_Chr4g0388831
rosa_laevigata RLG00000010022
rosa_multiflora Rmu_sc0000697.1_g000012 Rmu_sc0016023.1_g000003
rosa_rugosa Rorug03G0320200
rosa_samantha Rh4BG018000 Rh4CG027700 Rh4DG018600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 2 cut(s) 274, 394
AciI CCGC 7 cut(s) 215, 449, 452, 479, 506, 515, 540
AclWI GGATC 2 cut(s) 335, 348
AcuI CTGAAG 2 cut(s) 270, 327
AfaI GTAC 4 cut(s) 76, 88, 255, 369
AfiI CCNNNNNNNGG 4 cut(s) 221, 350, 431, 485
AgsI TTSAA 1 cut(s) 379
AjiI CACGTC 1 cut(s) 407
AjnI CCWGG 4 cut(s) 256, 430, 484, 535
AlwI GGATC 2 cut(s) 335, 348
AlwNI CAGNNNCTG 1 cut(s) 437
Ama87I CYCGRG 1 cut(s) 349
Aor13HI TCCGGA 2 cut(s) 274, 394
AoxI GGCC 3 cut(s) 233, 488, 542
ApeKI GCWGC 3 cut(s) 217, 454, 517
AspS9I GGNCC 4 cut(s) 353, 434, 488, 542
AsuC2I CCSGG 4 cut(s) 222, 338, 350, 351
AsuHPI GGTGA 2 cut(s) 99, 162
AvaI CYCGRG 1 cut(s) 349
AvaII GGWCC 2 cut(s) 353, 434
AxyI CCTNAGG 1 cut(s) 138
BamHI GGATCC 1 cut(s) 340
BauI CACGAG 1 cut(s) 176
BbvI GCAGC 3 cut(s) 204, 466, 529
BccI CCATC 1 cut(s) 17
BciT130I CCWGG 4 cut(s) 258, 432, 486, 537
BciVI GTATCC 2 cut(s) 73, 192
BcnI CCSGG 4 cut(s) 222, 338, 350, 351
BfaI CTAG 2 cut(s) 78, 546
BfuI GTATCC 2 cut(s) 73, 192
BglI GCCNNNNNGGC 1 cut(s) 487
BisI GCNGC 5 cut(s) 218, 449, 452, 455, 518
BlsI GCNGC 5 cut(s) 219, 450, 453, 456, 519
BmcAI AGTACT 1 cut(s) 76
Bme1390I CCNGG 8 cut(s) 222, 258, 338, 350, 351, 432, 486, 537
Bme18I GGWCC 2 cut(s) 353, 434
BmeT110I CYCGRG 1 cut(s) 349
BmgBI CACGTC 1 cut(s) 407
BmgT120I GGNCC 4 cut(s) 353, 434, 488, 542
BmiI GGNNCC 3 cut(s) 194, 342, 354
BmrFI CCNGG 8 cut(s) 222, 258, 338, 350, 351, 432, 486, 537
BpuMI CCSGG 4 cut(s) 222, 338, 350, 351
BsaJI CCNNGG 3 cut(s) 349, 485, 535
BsaWI WCCGGW 3 cut(s) 274, 394, 460
BsaXI ACNNNNNCTCC 2 cut(s) 283, 313
Bsc4I CCNNNNNNNGG 4 cut(s) 221, 350, 431, 485
Bse118I RCCGGY 1 cut(s) 231
Bse1I ACTGG 2 cut(s) 84, 365
Bse21I CCTNAGG 1 cut(s) 138
BseAI TCCGGA 2 cut(s) 274, 394
BseBI CCWGG 4 cut(s) 258, 432, 486, 537
BseDI CCNNGG 3 cut(s) 349, 485, 535
BseGI GGATG 1 cut(s) 403
BseLI CCNNNNNNNGG 4 cut(s) 221, 350, 431, 485
BseMII CTCAG 3 cut(s) 72, 152, 483
BseNI ACTGG 2 cut(s) 84, 365
BseRI GAGGAG 1 cut(s) 201
BseXI GCAGC 3 cut(s) 204, 466, 529
BshFI GGCC 3 cut(s) 235, 490, 544
BsiHKCI CYCGRG 1 cut(s) 349
BsiSI CCGG 8 cut(s) 200, 221, 232, 275, 338, 350, 395, 461
BslI CCNNNNNNNGG 4 cut(s) 221, 350, 431, 485
BsnI GGCC 3 cut(s) 235, 490, 544
BsoBI CYCGRG 1 cut(s) 349
Bsp13I TCCGGA 2 cut(s) 274, 394
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 7 cut(s) 215, 449, 452, 479, 506, 515, 540
BspANI GGCC 3 cut(s) 235, 490, 544
BspCNI CTCAG 3 cut(s) 71, 151, 484
BspEI TCCGGA 2 cut(s) 274, 394
BspLI GGNNCC 3 cut(s) 194, 342, 354
BspPI GGATC 2 cut(s) 335, 348
BsrFI RCCGGY 1 cut(s) 231
BsrI ACTGG 2 cut(s) 84, 365
BssAI RCCGGY 1 cut(s) 231
BssECI CCNNGG 3 cut(s) 349, 485, 535
BssMI GATC 1 cut(s) 340
BssSI CACGAG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 176
Bst2UI CCWGG 4 cut(s) 258, 432, 486, 537
BstC8I GCNNGC 3 cut(s) 197, 510, 542
BstDEI CTNAG 3 cut(s) 58, 138, 492
BstF5I GGATG 1 cut(s) 403
BstKTI GATC 1 cut(s) 343
BstMBI GATC 1 cut(s) 340
BstMWI GCNNNNNNNGC 4 cut(s) 454, 478, 487, 514
BstNI CCWGG 4 cut(s) 258, 432, 486, 537
BstSCI CCNGG 8 cut(s) 220, 256, 336, 348, 349, 430, 484, 535
BstV1I GCAGC 3 cut(s) 204, 466, 529
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
Bsu36I CCTNAGG 1 cut(s) 138
BsuI GTATCC 2 cut(s) 73, 192
BsuRI GGCC 3 cut(s) 235, 490, 544
BtrI CACGTC 1 cut(s) 407
BtsCI GGATG 1 cut(s) 403
Cac8I GCNNGC 3 cut(s) 197, 510, 542
CaiI CAGNNNCTG 1 cut(s) 437
Cfr10I RCCGGY 1 cut(s) 231
Cfr13I GGNCC 4 cut(s) 353, 434, 488, 542
Cfr9I CCCGGG 1 cut(s) 349
Csp6I GTAC 4 cut(s) 75, 87, 254, 368
CviAII CATG 1 cut(s) 152
CviJI RGCY 9 cut(s) 195, 199, 235, 315, 382, 457, 490, 520, 544
CviKI_1 RGCY 9 cut(s) 195, 199, 235, 315, 382, 457, 490, 520, 544
CviQI GTAC 4 cut(s) 75, 87, 254, 368
DdeI CTNAG 3 cut(s) 58, 138, 492
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 2 cut(s) 353, 434
Eco57I CTGAAG 2 cut(s) 270, 327
Eco81I CCTNAGG 1 cut(s) 138
Eco88I CYCGRG 1 cut(s) 349
EcoO109I RGGNCCY 1 cut(s) 434
EcoRII CCWGG 4 cut(s) 256, 430, 484, 535
EcoRV GATATC 1 cut(s) 286
FaeI CATG 1 cut(s) 155
FaiI YATR 2 cut(s) 153, 467
FatI CATG 1 cut(s) 151
FauI CCCGC 1 cut(s) 533
Fnu4HI GCNGC 5 cut(s) 218, 449, 452, 455, 518
FokI GGATG 1 cut(s) 410
Fsp4HI GCNGC 5 cut(s) 218, 449, 452, 455, 518
FspBI CTAG 2 cut(s) 78, 546
GluI GCNGC 5 cut(s) 218, 449, 452, 455, 518
HaeIII GGCC 3 cut(s) 235, 490, 544
HapII CCGG 8 cut(s) 200, 221, 232, 275, 338, 350, 395, 461
Hin1II CATG 1 cut(s) 155
HinfI GANTC 2 cut(s) 167, 427
HpaII CCGG 8 cut(s) 200, 221, 232, 275, 338, 350, 395, 461
HphI GGTGA 2 cut(s) 99, 162
Hpy188I TCNGA 3 cut(s) 166, 192, 345
Hpy188III TCNNGA 4 cut(s) 275, 356, 395, 416
HpyAV CCTTC 1 cut(s) 246
HpyCH4IV ACGT 1 cut(s) 406
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 4 cut(s) 454, 478, 487, 514
HpyF3I CTNAG 3 cut(s) 58, 138, 492
HpySE526I ACGT 1 cut(s) 406
Hsp92II CATG 1 cut(s) 155
Kpn2I TCCGGA 2 cut(s) 274, 394
Kzo9I GATC 1 cut(s) 340
LmnI GCTCC 1 cut(s) 192
Lsp1109I GCAGC 3 cut(s) 204, 466, 529
MaeI CTAG 2 cut(s) 78, 546
MaeII ACGT 1 cut(s) 406
MaeIII GTNAC 1 cut(s) 105
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MflI RGATCY 1 cut(s) 340
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 209
MnlI CCTC 9 cut(s) 102, 147, 222, 280, 333, 381, 396, 483, 492
MroI TCCGGA 2 cut(s) 274, 394
MspA1I CMGCKG 1 cut(s) 217
MspI CCGG 8 cut(s) 200, 221, 232, 275, 338, 350, 395, 461
MspR9I CCNGG 8 cut(s) 222, 258, 338, 350, 351, 432, 486, 537
MvaI CCWGG 4 cut(s) 258, 432, 486, 537
MwoI GCNNNNNNNGC 4 cut(s) 454, 478, 487, 514
NciI CCSGG 4 cut(s) 222, 338, 350, 351
NdeII GATC 1 cut(s) 340
NlaIII CATG 1 cut(s) 155
NlaIV GGNNCC 3 cut(s) 194, 342, 354
NmuCI GTSAC 1 cut(s) 105
PfeI GAWTC 1 cut(s) 427
PfoI TCCNGGA 1 cut(s) 430
PkrI GCNGC 5 cut(s) 219, 450, 453, 456, 519
PleI GAGTC 1 cut(s) 161
PpsI GAGTC 1 cut(s) 161
PpuMI RGGWCCY 1 cut(s) 434
Psp5II RGGWCCY 1 cut(s) 434
Psp6I CCWGG 4 cut(s) 256, 430, 484, 535
PspGI CCWGG 4 cut(s) 256, 430, 484, 535
PspN4I GGNNCC 3 cut(s) 194, 342, 354
PspPI GGNCC 4 cut(s) 353, 434, 488, 542
PspPPI RGGWCCY 1 cut(s) 434
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 4 cut(s) 76, 88, 255, 369
RsaNI GTAC 4 cut(s) 75, 87, 254, 368
SatI GCNGC 5 cut(s) 218, 449, 452, 455, 518
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 4 cut(s) 353, 434, 488, 542
ScaI AGTACT 1 cut(s) 76
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 8 cut(s) 222, 258, 338, 350, 351, 432, 486, 537
SinI GGWCC 2 cut(s) 353, 434
SmaI CCCGGG 1 cut(s) 351
SsiI CCGC 7 cut(s) 215, 449, 452, 479, 506, 515, 540
SspMI CTAG 2 cut(s) 78, 546
StyD4I CCNGG 8 cut(s) 220, 256, 336, 348, 349, 430, 484, 535
TaiI ACGT 1 cut(s) 409
TaqI TCGA 2 cut(s) 133, 157
TatI WGTACW 1 cut(s) 74
TauI GCSGC 2 cut(s) 451, 454
TfiI GAWTC 1 cut(s) 427
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 3 cut(s) 217, 454, 517
Tsp45I GTSAC 1 cut(s) 105
TspMI CCCGGG 1 cut(s) 349
VpaK11BI GGWCC 2 cut(s) 353, 434
XmaI CCCGGG 1 cut(s) 349
XspI CTAG 2 cut(s) 78, 546
ZrmI AGTACT 1 cut(s) 76
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.