Rh4CG027700

Vegetative cell wall protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0018529
Physical Location & Seq
Reverse (-)
5271798 .. 5272847
1050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG027700.1

Sequence Viewer

Length: 564 bp
ATGCAAAAGGTTACAATAATGTCGTCCATCTCTGCAATCTCATTCACACTCATAAAACGAACCATTCCACTTTGCTCAGTATCCAAAACAAGTACTAGCACCAGTACCCCTCCATTGTTATTGTCACTCAAGAACCCTATCTTTCCCACTCAACCTCAGATTTTAGTCCATGCTCGATTATCTGACTCACCCAACGAGTATCCAACTCGGAGCCCGCCGGAGTTTTCTCCTCCGGTGCCTGGTTTTGACCGGCCTTCTGTTACACCCGAAGTACCTGGAATATCTACCTCTCCGGAAGTTGACATCCCAACAACTACTCCACCTGAGGTCAGCCCTTATCCTCCACCACTTGACCCCGGTCCGGATTTTCCAGTACCTCCTTTGAAGCCACCTCCTGTTCCGGATGTGCCACGTCCTTTTCCAGATAACCCAATTCCAGGACCTGACAGACTTCCGCCGCAACCACCGGATATAGTGCCTCCACCGCCTCCTGGGCCTGAGATTATTCCACCGCCAGCACCGCCGCCACCGCCTACCGGTCCAGGCGGGCCTATTGTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

187

Amino Acids

19.67

Weight (kDa)

4.83

Isoelectric Point (pI)

73.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018529)

Species Orthologous Gene IDs
prunus_persica Prupe.1G023900_v2.0.a1
pyrus_communis pycom13g17550
rosa_chinensis RchiOBHm_Chr4g0388831
rosa_laevigata RLG00000010022
rosa_multiflora Rmu_sc0000697.1_g000012 Rmu_sc0016023.1_g000003
rosa_rugosa Rorug03G0320200
rosa_samantha Rh4BG018000 Rh4CG027700 Rh4DG018600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 235
AccIII TCCGGA 3 cut(s) 292, 361, 400
AciI CCGC 9 cut(s) 215, 455, 458, 485, 512, 521, 524, 530, 546
AfaI GTAC 4 cut(s) 94, 106, 273, 375
AfiI CCNNNNNNNGG 5 cut(s) 239, 361, 437, 491, 536
AgeI ACCGGT 1 cut(s) 536
AgsI TTSAA 1 cut(s) 385
AjiI CACGTC 1 cut(s) 413
AjnI CCWGG 5 cut(s) 238, 274, 436, 490, 541
AlwNI CAGNNNCTG 1 cut(s) 443
Aor13HI TCCGGA 3 cut(s) 292, 361, 400
AoxI GGCC 3 cut(s) 251, 494, 548
AsiGI ACCGGT 1 cut(s) 536
AspS9I GGNCC 5 cut(s) 359, 440, 494, 539, 548
AsuC2I CCSGG 1 cut(s) 357
AsuHPI GGTGA 1 cut(s) 180
AvaII GGWCC 3 cut(s) 359, 440, 539
AxyI CCTNAGG 1 cut(s) 324
BanI GGYRCC 1 cut(s) 235
BanII GRGCYC 1 cut(s) 215
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 5 cut(s) 240, 276, 438, 492, 543
BciVI GTATCC 2 cut(s) 91, 210
BcnI CCSGG 1 cut(s) 357
BfaI CTAG 1 cut(s) 96
BfuI GTATCC 2 cut(s) 91, 210
BglI GCCNNNNNGGC 1 cut(s) 493
BisI GCNGC 2 cut(s) 458, 524
BlsI GCNGC 2 cut(s) 459, 525
BmcAI AGTACT 1 cut(s) 94
Bme1390I CCNGG 6 cut(s) 240, 276, 357, 438, 492, 543
Bme18I GGWCC 3 cut(s) 359, 440, 539
BmgBI CACGTC 1 cut(s) 413
BmgT120I GGNCC 5 cut(s) 359, 440, 494, 539, 548
BmiI GGNNCC 2 cut(s) 212, 237
BmrFI CCNGG 6 cut(s) 240, 276, 357, 438, 492, 543
BoxI GACNNNNGTC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 113
BpuMI CCSGG 1 cut(s) 357
BsaJI CCNNGG 2 cut(s) 355, 491
BsaWI WCCGGW 6 cut(s) 232, 292, 361, 400, 466, 536
BsaXI ACNNNNNCTCC 2 cut(s) 301, 331
Bsc4I CCNNNNNNNGG 5 cut(s) 239, 361, 437, 491, 536
Bse118I RCCGGY 2 cut(s) 249, 536
Bse1I ACTGG 2 cut(s) 102, 371
Bse21I CCTNAGG 1 cut(s) 324
BseAI TCCGGA 3 cut(s) 292, 361, 400
BseBI CCWGG 5 cut(s) 240, 276, 438, 492, 543
BseDI CCNNGG 2 cut(s) 355, 491
BseGI GGATG 2 cut(s) 303, 409
BseLI CCNNNNNNNGG 5 cut(s) 239, 361, 437, 491, 536
BseMII CTCAG 4 cut(s) 90, 170, 315, 489
BseNI ACTGG 2 cut(s) 102, 371
BseRI GAGGAG 1 cut(s) 219
BshFI GGCC 3 cut(s) 253, 496, 550
BshNI GGYRCC 1 cut(s) 235
BshTI ACCGGT 1 cut(s) 536
BsiSI CCGG 9 cut(s) 218, 233, 250, 293, 357, 362, 401, 467, 537
BslI CCNNNNNNNGG 5 cut(s) 239, 361, 437, 491, 536
BsnI GGCC 3 cut(s) 253, 496, 550
Bsp1286I GDGCHC 1 cut(s) 215
Bsp13I TCCGGA 3 cut(s) 292, 361, 400
BspACI CCGC 9 cut(s) 215, 455, 458, 485, 512, 521, 524, 530, 546
BspANI GGCC 3 cut(s) 253, 496, 550
BspCNI CTCAG 4 cut(s) 89, 169, 316, 490
BspEI TCCGGA 3 cut(s) 292, 361, 400
BspLI GGNNCC 2 cut(s) 212, 237
BspT107I GGYRCC 1 cut(s) 235
BsrFI RCCGGY 2 cut(s) 249, 536
BsrI ACTGG 2 cut(s) 102, 371
BssAI RCCGGY 2 cut(s) 249, 536
BssECI CCNNGG 2 cut(s) 355, 491
Bst2UI CCWGG 5 cut(s) 240, 276, 438, 492, 543
BstC8I GCNNGC 3 cut(s) 215, 516, 548
BstDEI CTNAG 4 cut(s) 76, 156, 324, 498
BstF5I GGATG 2 cut(s) 303, 409
BstMWI GCNNNNNNNGC 4 cut(s) 484, 493, 520, 529
BstNI CCWGG 5 cut(s) 240, 276, 438, 492, 543
BstPAI GACNNNNGTC 1 cut(s) 357
BstSCI CCNGG 6 cut(s) 238, 274, 355, 436, 490, 541
Bsu36I CCTNAGG 1 cut(s) 324
BsuI GTATCC 2 cut(s) 91, 210
BsuRI GGCC 3 cut(s) 253, 496, 550
BtrI CACGTC 1 cut(s) 413
BtsCI GGATG 2 cut(s) 303, 409
Cac8I GCNNGC 3 cut(s) 215, 516, 548
CaiI CAGNNNCTG 1 cut(s) 443
Cfr10I RCCGGY 2 cut(s) 249, 536
Cfr13I GGNCC 5 cut(s) 359, 440, 494, 539, 548
CpoI CGGWCCG 1 cut(s) 359
Csp6I GTAC 4 cut(s) 93, 105, 272, 374
CspAI ACCGGT 1 cut(s) 536
CspI CGGWCCG 1 cut(s) 359
CviAII CATG 1 cut(s) 170
CviJI RGCY 6 cut(s) 213, 253, 333, 388, 496, 550
CviKI_1 RGCY 6 cut(s) 213, 253, 333, 388, 496, 550
CviQI GTAC 4 cut(s) 93, 105, 272, 374
DdeI CTNAG 4 cut(s) 76, 156, 324, 498
EciI GGCGGA 1 cut(s) 444
Eco24I GRGCYC 1 cut(s) 215
Eco47I GGWCC 3 cut(s) 359, 440, 539
Eco81I CCTNAGG 1 cut(s) 324
EcoO109I RGGNCCY 1 cut(s) 440
EcoRII CCWGG 5 cut(s) 238, 274, 436, 490, 541
EcoT38I GRGCYC 1 cut(s) 215
FaeI CATG 1 cut(s) 173
FaiI YATR 3 cut(s) 53, 171, 473
FatI CATG 1 cut(s) 169
FauI CCCGC 2 cut(s) 222, 539
Fnu4HI GCNGC 2 cut(s) 458, 524
FokI GGATG 2 cut(s) 290, 416
FriOI GRGCYC 1 cut(s) 215
Fsp4HI GCNGC 2 cut(s) 458, 524
FspBI CTAG 1 cut(s) 96
GluI GCNGC 2 cut(s) 458, 524
HaeIII GGCC 3 cut(s) 253, 496, 550
HapII CCGG 9 cut(s) 218, 233, 250, 293, 357, 362, 401, 467, 537
Hin1II CATG 1 cut(s) 173
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 1 cut(s) 185
HpaII CCGG 9 cut(s) 218, 233, 250, 293, 357, 362, 401, 467, 537
HphI GGTGA 1 cut(s) 180
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 159, 184, 210
Hpy188III TCNNGA 5 cut(s) 130, 293, 362, 401, 422
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 264
HpyCH4IV ACGT 1 cut(s) 412
HpyCH4V TGCA 2 cut(s) 4, 35
HpyF10VI GCNNNNNNNGC 4 cut(s) 484, 493, 520, 529
HpyF3I CTNAG 4 cut(s) 76, 156, 324, 498
HpySE526I ACGT 1 cut(s) 412
Hsp92II CATG 1 cut(s) 173
Kpn2I TCCGGA 3 cut(s) 292, 361, 400
LmnI GCTCC 1 cut(s) 210
MaeI CTAG 1 cut(s) 96
MaeII ACGT 1 cut(s) 412
MaeIII GTNAC 3 cut(s) 10, 123, 259
MhlI GDGCHC 1 cut(s) 215
MluCI AATT 1 cut(s) 432
MlyI GAGTC 1 cut(s) 179
MmeI TCCRAC 1 cut(s) 227
MroI TCCGGA 3 cut(s) 292, 361, 400
MspI CCGG 9 cut(s) 218, 233, 250, 293, 357, 362, 401, 467, 537
MspR9I CCNGG 6 cut(s) 240, 276, 357, 438, 492, 543
MvaI CCWGG 5 cut(s) 240, 276, 438, 492, 543
MwoI GCNNNNNNNGC 4 cut(s) 484, 493, 520, 529
NciI CCSGG 1 cut(s) 357
NlaIII CATG 1 cut(s) 173
NlaIV GGNNCC 2 cut(s) 212, 237
NmuCI GTSAC 1 cut(s) 123
PfoI TCCNGGA 1 cut(s) 436
PinAI ACCGGT 1 cut(s) 536
PkrI GCNGC 2 cut(s) 459, 525
PleI GAGTC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 179
PpuMI RGGWCCY 1 cut(s) 440
PshAI GACNNNNGTC 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 440
Psp6I CCWGG 5 cut(s) 238, 274, 436, 490, 541
PspGI CCWGG 5 cut(s) 238, 274, 436, 490, 541
PspN4I GGNNCC 2 cut(s) 212, 237
PspPI GGNCC 5 cut(s) 359, 440, 494, 539, 548
PspPPI RGGWCCY 1 cut(s) 440
PstNI CAGNNNCTG 1 cut(s) 443
RsaI GTAC 4 cut(s) 94, 106, 273, 375
RsaNI GTAC 4 cut(s) 93, 105, 272, 374
Rsr2I CGGWCCG 1 cut(s) 359
RsrII CGGWCCG 1 cut(s) 359
SatI GCNGC 2 cut(s) 458, 524
Sau96I GGNCC 5 cut(s) 359, 440, 494, 539, 548
ScaI AGTACT 1 cut(s) 94
SchI GAGTC 1 cut(s) 179
ScrFI CCNGG 6 cut(s) 240, 276, 357, 438, 492, 543
SduI GDGCHC 1 cut(s) 215
SinI GGWCC 3 cut(s) 359, 440, 539
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 1 cut(s) 432
SsiI CCGC 9 cut(s) 215, 455, 458, 485, 512, 521, 524, 530, 546
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 6 cut(s) 238, 274, 355, 436, 490, 541
TaiI ACGT 1 cut(s) 415
TaqI TCGA 1 cut(s) 175
TasI AATT 1 cut(s) 432
TatI WGTACW 1 cut(s) 92
TauI GCSGC 2 cut(s) 460, 526
TseFI GTSAC 1 cut(s) 123
Tsp45I GTSAC 1 cut(s) 123
VpaK11BI GGWCC 3 cut(s) 359, 440, 539
XspI CTAG 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 94
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.