RchiOBHm_Chr4g0388871

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018017
Physical Location & Seq
Forward (+)
4033446 .. 4034699
1254 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ36204

Sequence Viewer

Length: 1254 bp
ATGTTATGTAATTGTGTTGGTGCTACTGCTTGTCTGAAGAGAATTCGCTACCGTGTTAAACATTCCTATTCAACGATAAAGATGTACTTTTCGGGGCATGGGAAGCTTAAGTCTATGAAGAACAGGATCATGGAGGTTCTTGAAAGCATTATGCCTCTTGCACAAGAGGAGCGTCGAACTACTGTTGCCAAAATATTCAATAAGCTTACTTCAATGCCTCAAGACGCTAATTTTATCCATGTTGACATATATGATGTGACTGAGCCTGAGCCTAAGCCTAAGCCTGCAACCAGGTCAGCCATGGTAAATGAGTTGGAAAAAGTGAGACTTGATAGATCATGTAGTATTTGCATGGACGAATTTGATAAAGGTGTTATAGCTATTCGCTTGCCCTGCTTACACATTTTTCATGGAAATTGCATTTTCCGGTGGCTGGAGACGGATCATAAGTGTCCTTTGTGCCGAAATCCAATGCCTCACGATGATGTAGATCAGTATTCCTATGAACCCGACGACACTAGTCCTTCGGCGACCGTATACCAGCATTCTTATTATACTGAAATTGAGTCAAAAACATGCACACCAGCACCATCATCCATGCTTCAGATCCGATTCAATCTCTACAAGTCAAACCAAGATGGTTTATTACGGGTCAACGGTGAAGTTTCACGCACAATAGATACTGGACAAATCTACCTCGAAACTGAGCCACAATTACTTACAATTCCTAGTCGTTGCGAGCTGCAGCCAACTATGATCACCCAAGTGCTTAGAAGCATGAGTGTTCCGCGGCATGAGCAGCCACCCATTGTTGCCAAAATATTTGAGACGGTTGCCAGCAACAATTCTGATGGTGGTGGCGGTGCTTTTATTAATGCGGACATCTACGATGAGACTTTGAAATTGTTCGATCTTATGGCCTTTGCTCGACGGCTTTTCAGGATTAGACGTGTTGGAATTGATCGTCCCATCACAGACGAAATAGATGCTAGGGTTGCAAGGGAATTAGCTGAGGAGGCATTATTAGGAAAGCCTAGCACTAAGCCTGCAACTAAATCAGCCATTGAGGGGTTGGAGAGAGTGAGAGTTGATAAATTGTGTGGTATTTGTATGGACGACTTTGAGGATGGTCTAATAGCTACAATAGCTACTCGCTTCCCATGCTTACACATTTTTCATGGACATTGCATTCTCCGGTGGCTCGAGACCGATCACAAGTGTCCTTTGTGCCGATACCCATTGCTTAATTTGTGA

Protein Analysis

417

Amino Acids

47.54

Weight (kDa)

6.88

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-ANAPC11 PF12861 107 - 158 1e-05 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-rbx1 PF12678 108 - 155 4.7e-09 RING-H2 zinc finger domain
zf-RING_2 PF13639 113 - 155 2.5e-14 Ring finger domain
zf-C3HC4_2 PF13923 114 - 154 1.2e-10 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 114 - 154 4.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 114 - 141 5.7e-08 RING-like zinc finger
zf-RING_UBOX PF13445 114 - 152 7.2e-07 RING-type zinc-finger
zf-RING_2 PF13639 366 - 411 3.5e-12 Ring finger domain
zf-C3HC4_2 PF13923 367 - 410 3.5e-10 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 367 - 411 1e-07 RING-H2 zinc finger domain
zf-C3HC4 PF00097 367 - 410 1.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 537
AccII CGCG 1 cut(s) 790
AciI CCGC 4 cut(s) 788, 790, 861, 878
AclWI GGATC 3 cut(s) 134, 450, 601
AcsI RAATTY 2 cut(s) 42, 359
AcuI CTGAAG 2 cut(s) 56, 587
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 2 cut(s) 433, 1068
AflII CTTAAG 1 cut(s) 107
AflIII ACRYGT 1 cut(s) 949
AgsI TTSAA 6 cut(s) 72, 143, 199, 213, 616, 901
AhlI ACTAGT 1 cut(s) 518
AjiI CACGTC 1 cut(s) 950
AjnI CCWGG 1 cut(s) 290
AleI CACNNNNGTG 1 cut(s) 764
AluBI AGCT 7 cut(s) 106, 205, 380, 742, 1010, 1139, 1148
AluI AGCT 7 cut(s) 106, 205, 380, 742, 1010, 1139, 1148
Alw26I GTCTC 5 cut(s) 319, 431, 821, 887, 1199
AlwI GGATC 3 cut(s) 134, 450, 601
Ama87I CYCGRG 1 cut(s) 1202
AoxI GGCC 1 cut(s) 918
ApeKI GCWGC 3 cut(s) 742, 745, 799
ApoI RAATTY 2 cut(s) 42, 359
AseI ATTAAT 1 cut(s) 873
Asp700I GAANNNNTTC 1 cut(s) 905
AsuHPI GGTGA 2 cut(s) 671, 751
AvaI CYCGRG 1 cut(s) 1202
BbvCI CCTCAGC 1 cut(s) 1011
BbvI GCAGC 3 cut(s) 729, 757, 811
BccI CCATC 5 cut(s) 598, 632, 845, 977, 1121
BceAI ACGGC 1 cut(s) 947
BcgI CGANNNNNNTGC 2 cut(s) 968, 1002
BciT130I CCWGG 1 cut(s) 292
BclI TGATCA 1 cut(s) 756
BcoDI GTCTC 5 cut(s) 319, 431, 821, 887, 1199
BcuI ACTAGT 1 cut(s) 518
BfaI CTAG 4 cut(s) 519, 729, 990, 1035
BfmI CTRYAG 1 cut(s) 743
BfrI CTTAAG 1 cut(s) 107
BisI GCNGC 4 cut(s) 743, 746, 791, 800
BlsI GCNGC 4 cut(s) 744, 747, 792, 801
Bme1390I CCNGG 1 cut(s) 292
BmeT110I CYCGRG 1 cut(s) 1202
BmgBI CACGTC 1 cut(s) 950
BmrFI CCNGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 976
BoxI GACNNNNGTC 1 cut(s) 519
BpmI CTGGAG 1 cut(s) 455
Bpu10I CCTNAGC 4 cut(s) 267, 273, 279, 1011
BpuEI CTTGAG 1 cut(s) 204
BsaBI GATNNNNATC 1 cut(s) 489
BsaI GGTCTC 1 cut(s) 1199
BsaJI CCNNGG 2 cut(s) 300, 788
BsaWI WCCGGW 2 cut(s) 426, 1194
Bsc4I CCNNNNNNNGG 2 cut(s) 433, 1068
Bse1I ACTGG 1 cut(s) 688
Bse3DI GCAATG 2 cut(s) 1183, 1238
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 1 cut(s) 292
BseDI CCNNGG 2 cut(s) 300, 788
BseGI GGATG 2 cut(s) 593, 1132
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 2 cut(s) 433, 1068
BseMI GCAATG 2 cut(s) 1183, 1238
BseMII CTCAG 4 cut(s) 252, 258, 696, 1002
BseNI ACTGG 1 cut(s) 688
BseRI GAGGAG 2 cut(s) 182, 1028
BseXI GCAGC 3 cut(s) 729, 757, 811
Bsh1236I CGCG 1 cut(s) 790
Bsh1285I CGRYCG 1 cut(s) 534
BshFI GGCC 1 cut(s) 920
BsiEI CGRYCG 1 cut(s) 534
BsiHKCI CYCGRG 1 cut(s) 1202
BsiSI CCGG 2 cut(s) 427, 1195
BslFI GGGAC 1 cut(s) 951
BslI CCNNNNNNNGG 2 cut(s) 433, 1068
BsmAI GTCTC 5 cut(s) 319, 431, 821, 887, 1199
BsmBI CGTCTC 2 cut(s) 431, 821
BsmFI GGGAC 1 cut(s) 951
BsmI GAATGC 2 cut(s) 544, 1188
BsnI GGCC 1 cut(s) 920
Bso31I GGTCTC 1 cut(s) 1199
BsoBI CYCGRG 1 cut(s) 1202
Bsp143I GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
Bsp19I CCATGG 1 cut(s) 300
BspACI CCGC 4 cut(s) 788, 790, 861, 878
BspANI GGCC 1 cut(s) 920
BspCNI CTCAG 4 cut(s) 253, 259, 697, 1003
BspFNI CGCG 1 cut(s) 790
BspMAI CTGCAG 1 cut(s) 747
BspPI GGATC 3 cut(s) 134, 450, 601
BspTI CTTAAG 1 cut(s) 107
BspTNI GGTCTC 1 cut(s) 1199
BsrDI GCAATG 2 cut(s) 1183, 1238
BsrI ACTGG 1 cut(s) 688
BssECI CCNNGG 2 cut(s) 300, 788
BssMI GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
BssNAI GTATAC 1 cut(s) 538
BssT1I CCWWGG 1 cut(s) 300
Bst1107I GTATAC 1 cut(s) 538
Bst2UI CCWGG 1 cut(s) 292
Bst4CI ACNGT 5 cut(s) 53, 184, 535, 659, 832
Bst6I CTCTTC 1 cut(s) 32
BstAFI CTTAAG 1 cut(s) 107
BstC8I GCNNGC 5 cut(s) 285, 389, 740, 838, 1047
BstDEI CTNAG 8 cut(s) 261, 267, 273, 279, 705, 770, 1011, 1041
BstDSI CCRYGG 2 cut(s) 300, 788
BstF5I GGATG 2 cut(s) 593, 1132
BstFNI CGCG 1 cut(s) 790
BstKTI GATC 9 cut(s) 129, 338, 445, 493, 609, 759, 913, 964, 1213
BstMAI GTCTC 5 cut(s) 319, 431, 821, 887, 1199
BstMBI GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
BstMCI CGRYCG 1 cut(s) 534
BstMWI GCNNNNNNNGC 8 cut(s) 103, 393, 796, 799, 995, 1016, 1145, 1161
BstNI CCWGG 1 cut(s) 292
BstNSI RCATGY 1 cut(s) 579
BstPAI GACNNNNGTC 1 cut(s) 519
BstSCI CCNGG 1 cut(s) 290
BstSFI CTRYAG 1 cut(s) 743
BstUI CGCG 1 cut(s) 790
BstV1I GCAGC 3 cut(s) 729, 757, 811
BstX2I RGATCY 1 cut(s) 606
BstYI RGATCY 1 cut(s) 606
BstZ17I GTATAC 1 cut(s) 538
BsuRI GGCC 1 cut(s) 920
BtgI CCRYGG 2 cut(s) 300, 788
BtrI CACGTC 1 cut(s) 950
BtsCI GGATG 2 cut(s) 593, 1132
Cac8I GCNNGC 5 cut(s) 285, 389, 740, 838, 1047
Cfr42I CCGCGG 1 cut(s) 791
CseI GACGC 2 cut(s) 161, 233
CsiI ACCWGGT 1 cut(s) 290
Csp6I GTAC 1 cut(s) 85
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 8 cut(s) 261, 267, 273, 279, 705, 770, 1011, 1041
DpnI GATC 9 cut(s) 128, 337, 444, 492, 608, 758, 912, 963, 1212
DpnII GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco130I CCWWGG 1 cut(s) 300
Eco31I GGTCTC 1 cut(s) 1199
Eco57I CTGAAG 2 cut(s) 56, 587
Eco88I CYCGRG 1 cut(s) 1202
EcoRI GAATTC 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 290
EcoT14I CCWWGG 1 cut(s) 300
ErhI CCWWGG 1 cut(s) 300
Esp3I CGTCTC 2 cut(s) 431, 821
FalI AAGNNNNNCTT 4 cut(s) 71, 103, 312, 344
FaqI GGGAC 1 cut(s) 951
FbaI TGATCA 1 cut(s) 756
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 4 cut(s) 743, 746, 791, 800
FokI GGATG 2 cut(s) 580, 1139
Fsp4HI GCNGC 4 cut(s) 743, 746, 791, 800
FspBI CTAG 4 cut(s) 519, 729, 990, 1035
GluI GCNGC 4 cut(s) 743, 746, 791, 800
GsuI CTGGAG 1 cut(s) 455
HaeIII GGCC 1 cut(s) 920
HapII CCGG 2 cut(s) 427, 1195
HgaI GACGC 2 cut(s) 161, 233
HincII GTYRAC 2 cut(s) 244, 655
HindII GTYRAC 2 cut(s) 244, 655
HindIII AAGCTT 2 cut(s) 104, 203
HinfI GANTC 2 cut(s) 566, 612
HpaII CCGG 2 cut(s) 427, 1195
HphI GGTGA 2 cut(s) 671, 751
Hpy166II GTNNAC 3 cut(s) 244, 538, 655
Hpy188I TCNGA 4 cut(s) 36, 606, 611, 850
Hpy188III TCNNGA 5 cut(s) 140, 221, 479, 940, 1204
Hpy8I GTNNAC 3 cut(s) 244, 538, 655
Hpy99I CGWCG 3 cut(s) 177, 515, 933
HpyAV CCTTC 1 cut(s) 534
HpyCH4III ACNGT 5 cut(s) 53, 184, 535, 659, 832
HpyCH4IV ACGT 1 cut(s) 949
HpyCH4V TGCA 9 cut(s) 161, 287, 351, 420, 579, 745, 998, 1049, 1188
HpyF10VI GCNNNNNNNGC 8 cut(s) 103, 393, 796, 799, 995, 1016, 1145, 1161
HpyF3I CTNAG 8 cut(s) 261, 267, 273, 279, 705, 770, 1011, 1041
HpySE526I ACGT 1 cut(s) 949
Ksp22I TGATCA 1 cut(s) 756
KspI CCGCGG 1 cut(s) 791
Kzo9I GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
LmnI GCTCC 1 cut(s) 169
Lsp1109I GCAGC 3 cut(s) 729, 757, 811
LweI GCATC 1 cut(s) 976
MabI ACCWGGT 1 cut(s) 290
MaeI CTAG 4 cut(s) 519, 729, 990, 1035
MaeII ACGT 1 cut(s) 949
MaeIII GTNAC 1 cut(s) 256
MalI GATC 9 cut(s) 128, 337, 444, 492, 608, 758, 912, 963, 1212
MboI GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
MboII GAAGA 2 cut(s) 49, 130
MflI RGATCY 1 cut(s) 606
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 3 cut(s) 294, 934, 1053
MroXI GAANNNNTTC 1 cut(s) 905
MseI TTAA 4 cut(s) 57, 108, 873, 1245
MslI CAYNNNNRTG 2 cut(s) 483, 764
MspA1I CMGCKG 1 cut(s) 790
MspCI CTTAAG 1 cut(s) 107
MspI CCGG 2 cut(s) 427, 1195
MspR9I CCNGG 1 cut(s) 292
Mva1269I GAATGC 2 cut(s) 544, 1188
MvaI CCWGG 1 cut(s) 292
MvnI CGCG 1 cut(s) 790
MwoI GCNNNNNNNGC 8 cut(s) 103, 393, 796, 799, 995, 1016, 1145, 1161
NcoI CCATGG 1 cut(s) 300
NdeII GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
NmuCI GTSAC 1 cut(s) 256
NspI RCATGY 1 cut(s) 579
OliI CACNNNNGTG 1 cut(s) 764
PaeR7I CTCGAG 1 cut(s) 1202
PctI GAATGC 2 cut(s) 544, 1188
PdmI GAANNNNTTC 1 cut(s) 905
PfeI GAWTC 1 cut(s) 612
PkrI GCNGC 4 cut(s) 744, 747, 792, 801
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PshAI GACNNNNGTC 1 cut(s) 519
PshBI ATTAAT 1 cut(s) 873
Psp6I CCWGG 1 cut(s) 290
PspGI CCWGG 1 cut(s) 290
PstI CTGCAG 1 cut(s) 747
PsuI RGATCY 1 cut(s) 606
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 2 cut(s) 483, 764
SacII CCGCGG 1 cut(s) 791
SaqAI TTAA 4 cut(s) 57, 108, 873, 1245
SatI GCNGC 4 cut(s) 743, 746, 791, 800
Sau3AI GATC 9 cut(s) 126, 335, 442, 490, 606, 756, 910, 961, 1210
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 1 cut(s) 292
SexAI ACCWGGT 1 cut(s) 290
SfaNI GCATC 1 cut(s) 976
SfcI CTRYAG 1 cut(s) 743
Sfr274I CTCGAG 1 cut(s) 1202
Sfr303I CCGCGG 1 cut(s) 791
SgrBI CCGCGG 1 cut(s) 791
SlaI CTCGAG 1 cut(s) 1202
SmiMI CAYNNNNRTG 2 cut(s) 483, 764
SmlI CTYRAG 3 cut(s) 107, 219, 1202
SmoI CTYRAG 3 cut(s) 107, 219, 1202
SpeI ACTAGT 1 cut(s) 518
SsiI CCGC 4 cut(s) 788, 790, 861, 878
SspI AATATT 2 cut(s) 195, 822
SspMI CTAG 4 cut(s) 519, 729, 990, 1035
StyD4I CCNGG 1 cut(s) 290
StyI CCWWGG 1 cut(s) 300
TaaI ACNGT 5 cut(s) 53, 184, 535, 659, 832
TaiI ACGT 1 cut(s) 952
TaqI TCGA 5 cut(s) 175, 699, 909, 928, 1203
TaqII GACCGA 1 cut(s) 1223
TatI WGTACW 1 cut(s) 84
TauI GCSGC 1 cut(s) 793
TfiI GAWTC 1 cut(s) 612
Tru1I TTAA 4 cut(s) 57, 108, 873, 1245
Tru9I TTAA 4 cut(s) 57, 108, 873, 1245
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 3 cut(s) 742, 745, 799
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 4 cut(s) 131, 398, 519, 1166
TspGWI ACGGA 1 cut(s) 455
Vha464I CTTAAG 1 cut(s) 107
VspI ATTAAT 1 cut(s) 873
XapI RAATTY 2 cut(s) 42, 359
XceI RCATGY 1 cut(s) 579
XcmI CCANNNNNNNNNTGG 2 cut(s) 298, 1069
XhoI CTCGAG 1 cut(s) 1202
XmiI GTMKAC 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 905
XspI CTAG 4 cut(s) 519, 729, 990, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.