RchiOBHm_Chr5g0005971

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018018
Physical Location & Seq
Forward (+)
3729653 .. 3738109
8457 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28713

Sequence Viewer

Length: 222 bp
ATGAACCATGCAGTTGTTGAAGAGCATGCATTGGATGCAAAGGTACTTATTTTTAAGAAAAAGAGGAGGAAGAATTACCGTCGGACCAAAGGTCATCGCCAAGAATTAACCAAGTTGAGGATTATTGATATTCAAGGGATTCAGAAATCAGAACCTGTAGTCACCAAAAAACCTTCAAAGGCACCTGGTAAGAAGCCAGAAAAGGTTGCAGTTGCTGCATAG

Protein Analysis

73

Amino Acids

8.34

Weight (kDa)

10.56

Isoelectric Point (pI)

42.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 4 - 44 1.1e-12 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018018)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00950
malus_domestica MD05G1339700.v1.1
rosa_chinensis RchiOBHm_Chr5g0005971
rosa_laevigata RLG00000009255 RLG00000033578
rosa_roxburghii Rroxscaffold_3G00225690
rosa_rugosa Rorug07G0182000
rosa_samantha Rh2BG450200 Rh2BG450400 Rh2DG460100 Rh2DG460400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 181
AfaI GTAC 1 cut(s) 45
AfiI CCNNNNNNNGG 1 cut(s) 117
AgsI TTSAA 3 cut(s) 20, 134, 177
AhdI GACNNNNNGTC 1 cut(s) 90
AjnI CCWGG 1 cut(s) 184
AlwNI CAGNNNCTG 2 cut(s) 155, 215
ApeKI GCWGC 1 cut(s) 215
AspS9I GGNCC 1 cut(s) 84
AsuHPI GGTGA 1 cut(s) 154
AvaII GGWCC 1 cut(s) 84
BanI GGYRCC 1 cut(s) 181
BbvI GCAGC 1 cut(s) 202
BciT130I CCWGG 1 cut(s) 186
BfmI CTRYAG 1 cut(s) 156
BisI GCNGC 1 cut(s) 216
BlsI GCNGC 1 cut(s) 217
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 84
BmeRI GACNNNNNGTC 1 cut(s) 90
BmgT120I GGNCC 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 1 cut(s) 25
Bsc4I CCNNNNNNNGG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 186
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 117
BseRI GAGGAG 1 cut(s) 79
BseXI GCAGC 1 cut(s) 202
BshNI GGYRCC 1 cut(s) 181
BslI CCNNNNNNNGG 1 cut(s) 117
BspLI GGNNCC 1 cut(s) 183
BspQI GCTCTTC 1 cut(s) 15
BspT107I GGYRCC 1 cut(s) 181
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 15
BstAPI GCANNNNNTGC 2 cut(s) 35, 215
BstC8I GCNNGC 1 cut(s) 27
BstF5I GGATG 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 35, 215
BstNI CCWGG 1 cut(s) 186
BstNSI RCATGY 1 cut(s) 29
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 202
BtgZI GCGATG 1 cut(s) 80
BtsCI GGATG 1 cut(s) 40
Cac8I GCNNGC 1 cut(s) 27
CaiI CAGNNNCTG 2 cut(s) 155, 215
Cfr13I GGNCC 1 cut(s) 84
CsiI ACCWGGT 1 cut(s) 184
Csp6I GTAC 1 cut(s) 44
CviAII CATG 2 cut(s) 8, 26
CviJI RGCY 1 cut(s) 196
CviKI_1 RGCY 1 cut(s) 196
CviQI GTAC 1 cut(s) 44
DriI GACNNNNNGTC 1 cut(s) 90
Eam1104I CTCTTC 1 cut(s) 15
Eam1105I GACNNNNNGTC 1 cut(s) 90
EarI CTCTTC 1 cut(s) 15
Eco47I GGWCC 1 cut(s) 84
EcoRII CCWGG 1 cut(s) 184
EcoT22I ATGCAT 1 cut(s) 31
FaeI CATG 2 cut(s) 11, 29
FaiI YATR 3 cut(s) 9, 27, 220
FatI CATG 2 cut(s) 7, 25
Fnu4HI GCNGC 1 cut(s) 216
FokI GGATG 1 cut(s) 47
Fsp4HI GCNGC 1 cut(s) 216
GluI GCNGC 1 cut(s) 216
Hin1II CATG 2 cut(s) 11, 29
HinfI GANTC 1 cut(s) 139
HphI GGTGA 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 84, 144, 151
Hpy99I CGWCG 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 183
HpyCH4III ACNGT 1 cut(s) 80
HpyCH4V TGCA 5 cut(s) 11, 29, 38, 209, 218
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 215
Hsp92II CATG 2 cut(s) 11, 29
LguI GCTCTTC 1 cut(s) 15
LpnPI CCDG 4 cut(s) 168, 171, 198, 210
Lsp1109I GCAGC 1 cut(s) 202
LweI GCATC 1 cut(s) 25
MabI ACCWGGT 1 cut(s) 184
MaeIII GTNAC 1 cut(s) 160
MboII GAAGA 2 cut(s) 32, 82
MluCI AATT 2 cut(s) 73, 104
MmeI TCCRAC 1 cut(s) 62
MnlI CCTC 3 cut(s) 57, 60, 111
Mph1103I ATGCAT 1 cut(s) 31
MseI TTAA 2 cut(s) 54, 107
MspR9I CCNGG 1 cut(s) 186
MvaI CCWGG 1 cut(s) 186
MwoI GCNNNNNNNGC 2 cut(s) 35, 215
NlaIII CATG 2 cut(s) 11, 29
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 31
NspI RCATGY 1 cut(s) 29
PaeI GCATGC 1 cut(s) 29
PciSI GCTCTTC 1 cut(s) 15
PfeI GAWTC 1 cut(s) 139
PkrI GCNGC 1 cut(s) 217
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 1 cut(s) 84
PstNI CAGNNNCTG 2 cut(s) 155, 215
RsaI GTAC 1 cut(s) 45
RsaNI GTAC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 15
SaqAI TTAA 2 cut(s) 54, 107
SatI GCNGC 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 84
ScrFI CCNGG 1 cut(s) 186
SetI ASST 6 cut(s) 45, 94, 157, 175, 187, 207
SexAI ACCWGGT 1 cut(s) 184
SfaNI GCATC 1 cut(s) 25
SfcI CTRYAG 1 cut(s) 156
SgeI CNNG 9 cut(s) 20, 38, 113, 124, 146, 167, 197, 198, 209
SinI GGWCC 1 cut(s) 84
SphI GCATGC 1 cut(s) 29
Sse9I AATT 2 cut(s) 73, 104
StyD4I CCNGG 1 cut(s) 184
TaaI ACNGT 1 cut(s) 80
TasI AATT 2 cut(s) 73, 104
TfiI GAWTC 1 cut(s) 139
Tru1I TTAA 2 cut(s) 54, 107
Tru9I TTAA 2 cut(s) 54, 107
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 1 cut(s) 215
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 1 cut(s) 17
VpaK11BI GGWCC 1 cut(s) 84
XceI RCATGY 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.