Rh2BG450200

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018018
Physical Location & Seq
Forward (+)
63521651 .. 63533929
12279 bp
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UTR
Exon/CDS
Intron
Rh2BG450200.1

Sequence Viewer

Length: 630 bp
ATGGCCGGCAATGAGATCGAAGTTTGGATACCCAGAAAAACGAAGGGTGGAGATGATATTCCTCGTTACGAACACCGTGATTACTTTGCCATGAAGATTTATCATGGAGGGAACATATTTGAGAACAACAAGGTTTCTGATGCAAATCCGAAAGAGAAGGGGAAAGGAAAACTGTTTCCTGAGGCTAGGGCAGATGAGGTTAGTGGTTTAGATGAAGAGGGTTTACATGACATAGACATTCTTGACTATATGCAGTCTTTTGGTTGCATGCCTAGTCAAGAAAAGAGAGTTGAAGATGAGTTTGAGGAGGATGATAGTGAAAAGGATGAAGACTACATACCTGCTTTTGAGGATGAGGATTATGATTATGAGATTATTGATGATGAAGATGAAGTGTTAGATGAACAGAATATATGGTTGGAGGTTAATGCAATTGGCATTAGATGCAAAGGTACTATTTTTAAGAAAAAGAGGAGAAAGAATTACCGTCGGACCAAAGGTCATCGCCAGGAATTAACCAAGTTGAGGATTATTGATACTCAAGGGATTCAGAAACCAGAACCTGTAGTCACCGAAAAACCTCCAAAGGCATCTGGTAACCAGCCAGAAAAGGTTGCAATTGCTGCATAA

Protein Analysis

209

Amino Acids

24.18

Weight (kDa)

4.77

Isoelectric Point (pI)

51.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 132 - 178 3e-09 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018018)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00950
malus_domestica MD05G1339700.v1.1
rosa_chinensis RchiOBHm_Chr5g0005971
rosa_laevigata RLG00000009255 RLG00000033578
rosa_roxburghii Rroxscaffold_3G00225690
rosa_rugosa Rorug07G0182000
rosa_samantha Rh2BG450200 Rh2BG450400 Rh2DG460100 Rh2DG460400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 349
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 1 cut(s) 454
AfiI CCNNNNNNNGG 1 cut(s) 525
AgsI TTSAA 1 cut(s) 293
AhdI GACNNNNNGTC 1 cut(s) 498
AjnI CCWGG 1 cut(s) 507
AjuI GAANNNNNNNTTGG 2 cut(s) 401, 433
AlwNI CAGNNNCTG 1 cut(s) 563
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 623
AspS9I GGNCC 1 cut(s) 492
AsuHPI GGTGA 1 cut(s) 562
AvaII GGWCC 1 cut(s) 492
AxyI CCTNAGG 1 cut(s) 180
BarI GAAGNNNNNNTAC 6 cut(s) 12, 44, 207, 239, 321, 353
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 1 cut(s) 610
BcgI CGANNNNNNTGC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 509
BciVI GTATCC 1 cut(s) 21
BfaI CTAG 2 cut(s) 186, 273
BfmI CTRYAG 1 cut(s) 564
BfuAI ACCTGC 1 cut(s) 349
BfuI GTATCC 1 cut(s) 21
BisI GCNGC 1 cut(s) 624
BlsI GCNGC 1 cut(s) 625
Bme1390I CCNGG 1 cut(s) 509
Bme18I GGWCC 1 cut(s) 492
BmeRI GACNNNNNGTC 1 cut(s) 498
BmgT120I GGNCC 1 cut(s) 492
BmrFI CCNGG 1 cut(s) 509
BmsI GCATC 3 cut(s) 130, 434, 599
BpiI GAAGAC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 525
BsaBI GATNNNNATC 1 cut(s) 144
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse118I RCCGGY 1 cut(s) 5
Bse21I CCTNAGG 1 cut(s) 180
Bse3DI GCAATG 1 cut(s) 16
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 1 cut(s) 509
BseGI GGATG 3 cut(s) 316, 331, 358
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 2 cut(s) 320, 487
BseXI GCAGC 1 cut(s) 610
BshFI GGCC 1 cut(s) 5
BsiSI CCGG 1 cut(s) 6
BslI CCNNNNNNNGG 1 cut(s) 525
BsnI GGCC 1 cut(s) 5
Bsp143I GATC 1 cut(s) 15
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 1 cut(s) 172
BspMI ACCTGC 1 cut(s) 349
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 5
BssAI RCCGGY 1 cut(s) 5
BssMI GATC 1 cut(s) 15
Bst2UI CCWGG 1 cut(s) 509
Bst4CI ACNGT 3 cut(s) 77, 174, 488
Bst6I CTCTTC 1 cut(s) 210
BstAPI GCANNNNNTGC 2 cut(s) 444, 623
BstC8I GCNNGC 2 cut(s) 7, 269
BstDEI CTNAG 1 cut(s) 180
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 3 cut(s) 316, 331, 358
BstKTI GATC 1 cut(s) 18
BstMBI GATC 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 444, 623
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 271
BstPI GGTNACC 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 507
BstSFI CTRYAG 1 cut(s) 564
BstV1I GCAGC 1 cut(s) 610
BstV2I GAAGAC 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 180
BsuI GTATCC 1 cut(s) 21
BsuRI GGCC 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 488
BtsCI GGATG 3 cut(s) 316, 331, 358
BveI ACCTGC 1 cut(s) 349
Cac8I GCNNGC 2 cut(s) 7, 269
CaiI CAGNNNCTG 1 cut(s) 563
Cfr10I RCCGGY 1 cut(s) 5
Cfr13I GGNCC 1 cut(s) 492
Csp6I GTAC 1 cut(s) 453
CviAII CATG 4 cut(s) 91, 104, 227, 268
CviJI RGCY 3 cut(s) 5, 185, 604
CviKI_1 RGCY 3 cut(s) 5, 185, 604
CviQI GTAC 1 cut(s) 453
DdeI CTNAG 1 cut(s) 180
DpnI GATC 1 cut(s) 17
DpnII GATC 1 cut(s) 15
DriI GACNNNNNGTC 1 cut(s) 498
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 210
Eam1105I GACNNNNNGTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 210
Eco47I GGWCC 1 cut(s) 492
Eco81I CCTNAGG 1 cut(s) 180
Eco91I GGTNACC 1 cut(s) 596
EcoO65I GGTNACC 1 cut(s) 596
EcoRII CCWGG 1 cut(s) 507
FaeI CATG 4 cut(s) 94, 107, 230, 271
FatI CATG 4 cut(s) 90, 103, 226, 267
Fnu4HI GCNGC 1 cut(s) 624
FokI GGATG 3 cut(s) 323, 338, 365
Fsp4HI GCNGC 1 cut(s) 624
FspBI CTAG 2 cut(s) 186, 273
GluI GCNGC 1 cut(s) 624
HaeIII GGCC 1 cut(s) 5
HapII CCGG 1 cut(s) 6
Hin1II CATG 4 cut(s) 94, 107, 230, 271
HinfI GANTC 1 cut(s) 547
HpaII CCGG 1 cut(s) 6
HphI GGTGA 1 cut(s) 562
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 4 cut(s) 139, 150, 492, 552
Hpy188III TCNNGA 3 cut(s) 179, 242, 278
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 1 cut(s) 492
HpyAV CCTTC 2 cut(s) 37, 151
HpyCH4III ACNGT 3 cut(s) 77, 174, 488
HpyCH4V TGCA 7 cut(s) 143, 253, 267, 431, 447, 617, 626
HpyF10VI GCNNNNNNNGC 2 cut(s) 444, 623
HpyF3I CTNAG 1 cut(s) 180
Hsp92II CATG 4 cut(s) 94, 107, 230, 271
KroI GCCGGC 1 cut(s) 5
KroNI GCCGGC 1 cut(s) 7
Kzo9I GATC 1 cut(s) 15
Lsp1109I GCAGC 1 cut(s) 610
LweI GCATC 3 cut(s) 130, 434, 599
MaeI CTAG 2 cut(s) 186, 273
MaeIII GTNAC 3 cut(s) 65, 568, 596
MalI GATC 1 cut(s) 17
MboI GATC 1 cut(s) 15
MboII GAAGA 5 cut(s) 106, 227, 305, 341, 398
MfeI CAATTG 2 cut(s) 432, 618
MluCI AATT 4 cut(s) 432, 481, 512, 618
MmeI TCCRAC 2 cut(s) 399, 470
MroNI GCCGGC 1 cut(s) 5
MseI TTAA 3 cut(s) 426, 462, 515
MspI CCGG 1 cut(s) 6
MspR9I CCNGG 1 cut(s) 509
MunI CAATTG 2 cut(s) 432, 618
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 2 cut(s) 444, 623
NaeI GCCGGC 1 cut(s) 7
NdeII GATC 1 cut(s) 15
NgoMIV GCCGGC 1 cut(s) 5
NlaIII CATG 4 cut(s) 94, 107, 230, 271
NmuCI GTSAC 1 cut(s) 568
NspI RCATGY 1 cut(s) 271
PaeI GCATGC 1 cut(s) 271
PdiI GCCGGC 1 cut(s) 7
PfeI GAWTC 1 cut(s) 547
PkrI GCNGC 1 cut(s) 625
Psp6I CCWGG 1 cut(s) 507
PspEI GGTNACC 1 cut(s) 596
PspGI CCWGG 1 cut(s) 507
PspPI GGNCC 1 cut(s) 492
PstNI CAGNNNCTG 1 cut(s) 563
RsaI GTAC 1 cut(s) 454
RsaNI GTAC 1 cut(s) 453
SaqAI TTAA 3 cut(s) 426, 462, 515
SatI GCNGC 1 cut(s) 624
Sau3AI GATC 1 cut(s) 15
Sau96I GGNCC 1 cut(s) 492
ScrFI CCNGG 1 cut(s) 509
SetI ASST 9 cut(s) 135, 201, 343, 426, 454, 502, 565, 583, 615
SfaNI GCATC 3 cut(s) 130, 434, 599
SfcI CTRYAG 1 cut(s) 564
SinI GGWCC 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 540
SmoI CTYRAG 1 cut(s) 540
SphI GCATGC 1 cut(s) 271
Sse9I AATT 4 cut(s) 432, 481, 512, 618
SspMI CTAG 2 cut(s) 186, 273
StyD4I CCNGG 1 cut(s) 507
TaaI ACNGT 3 cut(s) 77, 174, 488
TaqI TCGA 1 cut(s) 18
TasI AATT 4 cut(s) 432, 481, 512, 618
TfiI GAWTC 1 cut(s) 547
Tru1I TTAA 3 cut(s) 426, 462, 515
Tru9I TTAA 3 cut(s) 426, 462, 515
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 1 cut(s) 623
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 6 cut(s) 107, 228, 342, 399, 405, 417
VpaK11BI GGWCC 1 cut(s) 492
XceI RCATGY 1 cut(s) 271
XspI CTAG 2 cut(s) 186, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.