Rh2DG460100

50S ribosomal protein L21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018018
Physical Location & Seq
Forward (+)
66441671 .. 66452398
10728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG460100.1

Sequence Viewer

Length: 363 bp
ATGCCTAGTCAAGAAAAGAGAGTTGAAGATGAGTTTGAGGAGGATGATAGTGAAAAGGATGAAGACTACATACCTGCTTTTGAGGATGAGGATTATGATTATGAGATTATTGATGATGAAGATGAAGTGTTAGATGAACAGAATATATGGTTGGAGGTTAATGCAATTGGCATTAGATGCAAAGGTACTATTTTTAAGAAAAAGAGGAGAAAGAATTACCGTCGGACCAAAGGTCATCGCCAGGAATTAACCAAGTTGAGGATTATTGATACTCAAGGGATTCAGAAACCAGAACCTGTAGTCACCGAAAAACCTCCAAAGGCATCTGGTAACCAGCCAGAAAAGGTTGCAATTGCTGCATAA

Protein Analysis

120

Amino Acids

14.08

Weight (kDa)

4.69

Isoelectric Point (pI)

62.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L21p PF00829 43 - 89 9.2e-10 Ribosomal prokaryotic L21 protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018018)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g00950
malus_domestica MD05G1339700.v1.1
rosa_chinensis RchiOBHm_Chr5g0005971
rosa_laevigata RLG00000009255 RLG00000033578
rosa_roxburghii Rroxscaffold_3G00225690
rosa_rugosa Rorug07G0182000
rosa_samantha Rh2BG450200 Rh2BG450400 Rh2DG460100 Rh2DG460400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 82
AfaI GTAC 1 cut(s) 187
AfiI CCNNNNNNNGG 1 cut(s) 258
AgsI TTSAA 1 cut(s) 26
AhdI GACNNNNNGTC 1 cut(s) 231
AjnI CCWGG 1 cut(s) 240
AjuI GAANNNNNNNTTGG 2 cut(s) 134, 166
AlwNI CAGNNNCTG 1 cut(s) 296
ApeKI GCWGC 1 cut(s) 356
AspS9I GGNCC 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 295
AvaII GGWCC 1 cut(s) 225
BarI GAAGNNNNNNTAC 2 cut(s) 54, 86
BbsI GAAGAC 1 cut(s) 69
BbvI GCAGC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 242
BfaI CTAG 1 cut(s) 6
BfmI CTRYAG 1 cut(s) 297
BfuAI ACCTGC 1 cut(s) 82
BisI GCNGC 1 cut(s) 357
BlsI GCNGC 1 cut(s) 358
Bme1390I CCNGG 1 cut(s) 242
Bme18I GGWCC 1 cut(s) 225
BmeRI GACNNNNNGTC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 225
BmrFI CCNGG 1 cut(s) 242
BmsI GCATC 2 cut(s) 167, 332
BpiI GAAGAC 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 1 cut(s) 258
BseBI CCWGG 1 cut(s) 242
BseGI GGATG 3 cut(s) 49, 64, 91
BseLI CCNNNNNNNGG 1 cut(s) 258
BseRI GAGGAG 2 cut(s) 53, 220
BseXI GCAGC 1 cut(s) 343
BslI CCNNNNNNNGG 1 cut(s) 258
BspMI ACCTGC 1 cut(s) 82
Bst2UI CCWGG 1 cut(s) 242
Bst4CI ACNGT 1 cut(s) 221
BstAPI GCANNNNNTGC 2 cut(s) 177, 356
BstEII GGTNACC 1 cut(s) 329
BstF5I GGATG 3 cut(s) 49, 64, 91
BstMWI GCNNNNNNNGC 2 cut(s) 177, 356
BstNI CCWGG 1 cut(s) 242
BstPI GGTNACC 1 cut(s) 329
BstSCI CCNGG 1 cut(s) 240
BstSFI CTRYAG 1 cut(s) 297
BstV1I GCAGC 1 cut(s) 343
BstV2I GAAGAC 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 221
BtsCI GGATG 3 cut(s) 49, 64, 91
BveI ACCTGC 1 cut(s) 82
CaiI CAGNNNCTG 1 cut(s) 296
Cfr13I GGNCC 1 cut(s) 225
Csp6I GTAC 1 cut(s) 186
CviJI RGCY 1 cut(s) 337
CviKI_1 RGCY 1 cut(s) 337
CviQI GTAC 1 cut(s) 186
DriI GACNNNNNGTC 1 cut(s) 231
Eam1105I GACNNNNNGTC 1 cut(s) 231
Eco47I GGWCC 1 cut(s) 225
Eco91I GGTNACC 1 cut(s) 329
EcoO65I GGTNACC 1 cut(s) 329
EcoRII CCWGG 1 cut(s) 240
FaiI YATR 6 cut(s) 71, 96, 102, 146, 148, 361
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 3 cut(s) 56, 71, 98
Fsp4HI GCNGC 1 cut(s) 357
FspBI CTAG 1 cut(s) 6
GluI GCNGC 1 cut(s) 357
HinfI GANTC 1 cut(s) 280
HphI GGTGA 1 cut(s) 295
Hpy188I TCNGA 2 cut(s) 225, 285
Hpy188III TCNNGA 1 cut(s) 11
Hpy99I CGWCG 1 cut(s) 225
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4V TGCA 4 cut(s) 164, 180, 350, 359
HpyF10VI GCNNNNNNNGC 2 cut(s) 177, 356
LpnPI CCDG 8 cut(s) 87, 227, 254, 303, 309, 312, 347, 351
Lsp1109I GCAGC 1 cut(s) 343
LweI GCATC 2 cut(s) 167, 332
MaeI CTAG 1 cut(s) 6
MaeIII GTNAC 2 cut(s) 301, 329
MboII GAAGA 3 cut(s) 38, 74, 131
MfeI CAATTG 2 cut(s) 165, 351
MluCI AATT 4 cut(s) 165, 214, 245, 351
MmeI TCCRAC 2 cut(s) 132, 203
MnlI CCTC 8 cut(s) 31, 34, 76, 82, 148, 198, 252, 324
MseI TTAA 3 cut(s) 159, 195, 248
MspR9I CCNGG 1 cut(s) 242
MunI CAATTG 2 cut(s) 165, 351
MvaI CCWGG 1 cut(s) 242
MwoI GCNNNNNNNGC 2 cut(s) 177, 356
NmuCI GTSAC 1 cut(s) 301
PfeI GAWTC 1 cut(s) 280
PkrI GCNGC 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 240
PspEI GGTNACC 1 cut(s) 329
PspGI CCWGG 1 cut(s) 240
PspPI GGNCC 1 cut(s) 225
PstNI CAGNNNCTG 1 cut(s) 296
RsaI GTAC 1 cut(s) 187
RsaNI GTAC 1 cut(s) 186
SaqAI TTAA 3 cut(s) 159, 195, 248
SatI GCNGC 1 cut(s) 357
Sau96I GGNCC 1 cut(s) 225
ScrFI CCNGG 1 cut(s) 242
SetI ASST 7 cut(s) 76, 159, 187, 235, 298, 316, 348
SfaNI GCATC 2 cut(s) 167, 332
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 1 cut(s) 225
SmlI CTYRAG 1 cut(s) 273
SmoI CTYRAG 1 cut(s) 273
Sse9I AATT 4 cut(s) 165, 214, 245, 351
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 1 cut(s) 240
TaaI ACNGT 1 cut(s) 221
TasI AATT 4 cut(s) 165, 214, 245, 351
TfiI GAWTC 1 cut(s) 280
Tru1I TTAA 3 cut(s) 159, 195, 248
Tru9I TTAA 3 cut(s) 159, 195, 248
TseFI GTSAC 1 cut(s) 301
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 301
TspDTI ATGAA 4 cut(s) 75, 132, 138, 150
VpaK11BI GGWCC 1 cut(s) 225
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.