RchiOBHm_Chr5g0016011

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0019014
Physical Location & Seq
Reverse (-)
11070758 .. 11072910
2153 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ29641

Sequence Viewer

Length: 2022 bp
ATGGGGTCTAATTCATTTTCCAGTTTCATTCCAGTCACGCTTTGTACCTTACCAATTCTTCAGTGGCTTCTCTTATCGCTTAACAATTTGACCATTGATACCTCCACTCCAGAAGCAAACATTTTCTCATGTTTAGCCAATCTTAGAAACTTGAGTATGTTGAACCTCGCAGGCAATCCTTTGAACACTGCTCTCCCTGCCTCCTTAGGGAATCTATCTACATCACTACAGTATATTGATTTTAGAAGATGCAACTTCAAGGGTGACATCCCCAAGGAGATAGGCAACTTGAGCAGCTTGATCAGCTTGCAACTAGGATTCAATCAACTGAGTGGATCACTTCCAACGACACTCGGAAGAGTACGGAATCTCCAAGGTATGTATTTGAGTGACAACAAATTGGTAAGCTATATCCCAGATGAACTTTGTCAACTATACAACTTAGCTGATATGGAAATGCCTCATAATCTCTTCTCTGGTTCTATACCTTCCTGCTTAGGAGATCTAGCCACATCACTAAATTTAATGTCCAATTTGTTAACTTCGACAGTATCATCTTTCTTGTGGCAACTTAAATATATCCTGGACTTAGACTTGTCATCTAATTCTTTGGTTGGATCTCTCTCAGAGGACATTGGTAATTTGAAAGTTGTAGCACATATAGATTTGTCAAACAACCATTTCTCTGGTTCTATACCAAGCAGCATTGGGGGTCTTCAAGATTTGTTTAGTCTCTCCTTGGCAAATAATAATCTAGAAGGCCATAGTCCTAGTTCATTTGGGAACTCGCTAAGCCTAGAACTCCTGGATCTATCCAACAATAGTCTTTCTGGAGTCATTCCGAAGTCTCTAGAAGCACTCTCATATCTCCAGTATCTCAATTTCTCTTTCAACAAATTGCTAGGAGAAATTCCAACAGGTGGTTATTTCAAAAACTTCTCTTCCGAATCTTTCGTCTCAAATGGTGCACTCTGTGGCGCGACCCGACTGCTTGTTCCAACGTGCAAAACGAAAGCAGCTAGCAGATATTTCGTGAAGTATATTACTATAGGCAGCTTGTCAGCCATTCTCCTTGTAGTCCTTGTATCAATGATACTATGCAGAAAAAGGATTGTGGAAGCTGCAACAGATCAGACTACCCTGTCACCTCAAAAACTTCTTTGGAGAAAAGTTTCACGCTTGGAACTTCTAAGTGCAACAAATGGATTTAGTGAATGCAATTTACTTGGCACTGGGGCTTTTGGCTCAGTATACAAAGGCACAATGTCAGATGGGATAGACGTTGCAATAAAGGTTTTCAACTTGGAAGTAGAAGGAGTTTCCAAGAGTTCTGATAATGAATGTGAAATGCTAAGCAATATTCGTTATATAAATCTTCTCAAAATCATCACTTGTTGCAGTCAAACAGATTTCAAAGCTGTTGTTGGATATAATGATAGATGTTGCATCGGCATTGGAATACCTTCATCACCTATCATCCATTGTGATCTGAAGCCCAGCAACATACTAATAGATGATGATATGGTTGCACATATTGCTGATTTTGGCATTGCAAAACTCCTAGGTGGTGGAAATTCTACGACTCATACCATAACCCTAGCAACTATTGGTTATATGGCTCCAGGTGGATATACTGTTGCTTGTTTATTTATAAAGTTACGGGATAAAAGGAATAGTTTCGAGAAGAGGGGATGTGTTTACAGCTTTGGTATTGTACTGATGGAAACATTCACAAAGAGGAAGCCAACAGACGAGATGTTTGGTAGGGAGGTGAGTATGAAGCAATGGGTTGCAGATTCACTATTTCCAGGTGGAATGGTGGAAGTTGTAGATGCCAATTTATTTGGGACTGAAGAAGACGATGATTTTGCGAGCAAGGAGTGTTTATCATCCCTTATGAGATTGGCTCTAGTGTGTTGTGCAGAATCGCCAGAAAAGAGGATAAGTATGCAAGATGCGCTAGCCATGCTCAACAAAATCAAGACCAAGTTTTTGAAGAACGCTGCAGGAGGTGCTGTGTAA

Protein Analysis

673

Amino Acids

72.96

Weight (kDa)

5.78

Isoelectric Point (pI)

37.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 39 - 179 3.1e-07 Leucine-rich repeat region
LRR_14 PF23598 188 - 299 2.2e-08 Leucine-rich repeat region
LRR_8 PF13855 244 - 300 1.1e-08 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 407 - 471 3.1e-06 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 490 - 653 2e-10 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 491 - 655 8.6e-17 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1652
AasI GACNNNNNNGTC 1 cut(s) 1141
AccB7I CCANNNNNTGG 1 cut(s) 920
AccI GTMKAC 1 cut(s) 1251
AccII CGCG 1 cut(s) 980
AclWI GGATC 3 cut(s) 343, 625, 816
AcsI RAATTY 3 cut(s) 520, 909, 1573
AcuI CTGAAG 3 cut(s) 44, 1511, 1872
AdeI CACNNNGTG 1 cut(s) 974
AfaI GTAC 3 cut(s) 46, 363, 1716
AfiI CCNNNNNNNGG 2 cut(s) 207, 920
AjnI CCWGG 4 cut(s) 582, 804, 1621, 1807
AluBI AGCT 9 cut(s) 297, 306, 408, 446, 1019, 1056, 1121, 1418, 1704
AluI AGCT 9 cut(s) 297, 306, 408, 446, 1019, 1056, 1121, 1418, 1704
Alw21I GWGCWC 1 cut(s) 970
Alw26I GTCTC 3 cut(s) 737, 852, 961
Alw44I GTGCAC 1 cut(s) 966
AlwI GGATC 3 cut(s) 343, 625, 816
AoxI GGCC 1 cut(s) 760
ApaLI GTGCAC 1 cut(s) 966
ApeKI GCWGC 6 cut(s) 294, 702, 1016, 1053, 1121, 2003
ApoI RAATTY 3 cut(s) 520, 909, 1573
ArsI GACNNNNNNTTYG 8 cut(s) 939, 971, 1742, 1774, 1850, 1882, 1975, 2007
Asp700I GAANNNNTTC 3 cut(s) 1171, 1462, 1676
AspA2I CCTAGG 1 cut(s) 1561
AspLEI GCGC 2 cut(s) 980, 1960
AsuHPI GGTGA 4 cut(s) 275, 1137, 1461, 1783
AsuNHI GCTAGC 2 cut(s) 1019, 1960
AvrII CCTAGG 1 cut(s) 1561
AxyI CCTNAGG 1 cut(s) 205
BaeGI GKGCMC 1 cut(s) 970
BbsI GAAGAC 2 cut(s) 707, 1863
Bbv12I GWGCWC 1 cut(s) 970
BbvI GCAGC 6 cut(s) 306, 714, 1028, 1065, 1108, 1990
BccI CCATC 2 cut(s) 1265, 1714
BcgI CGANNNNNNTGC 6 cut(s) 970, 1004, 1012, 1046, 1850, 1884
BciT130I CCWGG 4 cut(s) 584, 806, 1623, 1809
BclI TGATCA 1 cut(s) 300
BcoDI GTCTC 3 cut(s) 737, 852, 961
BfmI CTRYAG 3 cut(s) 227, 1047, 2004
BglII AGATCT 1 cut(s) 502
BisI GCNGC 6 cut(s) 295, 703, 1017, 1054, 1122, 2004
BlnI CCTAGG 1 cut(s) 1561
BlpI GCTNAGC 2 cut(s) 791, 1352
BlsI GCNGC 6 cut(s) 296, 704, 1018, 1055, 1123, 2005
Bme1390I CCNGG 4 cut(s) 584, 806, 1623, 1809
BmiI GGNNCC 1 cut(s) 1620
BmrFI CCNGG 4 cut(s) 584, 806, 1623, 1809
BmrI ACTGGG 1 cut(s) 1242
BmsI GCATC 4 cut(s) 239, 1455, 1822, 1945
BmtI GCTAGC 2 cut(s) 1023, 1964
BmuI ACTGGG 1 cut(s) 1242
BpiI GAAGAC 2 cut(s) 707, 1863
BplI GAGNNNNNCTC 2 cut(s) 1891, 1923
BpmI CTGGAG 4 cut(s) 93, 852, 854, 1605
Bpu10I CCTNAGC 1 cut(s) 496
Bpu1102I GCTNAGC 2 cut(s) 791, 1352
BpuEI CTTGAG 2 cut(s) 172, 310
BsaJI CCNNGG 4 cut(s) 273, 373, 738, 1561
BsaXI ACNNNNNCTCC 5 cut(s) 91, 121, 354, 384, 2001
Bsc4I CCNNNNNNNGG 2 cut(s) 207, 920
Bse1I ACTGG 4 cut(s) 21, 32, 871, 1237
Bse21I CCTNAGG 1 cut(s) 205
Bse3DI GCAATG 2 cut(s) 1548, 1790
BseBI CCWGG 4 cut(s) 584, 806, 1623, 1809
BseDI CCNNGG 4 cut(s) 273, 373, 738, 1561
BseGI GGATG 4 cut(s) 267, 1476, 1697, 1889
BseLI CCNNNNNNNGG 2 cut(s) 207, 920
BseMI GCAATG 2 cut(s) 1548, 1790
BseMII CTCAG 3 cut(s) 320, 639, 1260
BseNI ACTGG 4 cut(s) 21, 32, 871, 1237
BseSI GKGCMC 1 cut(s) 970
BseXI GCAGC 6 cut(s) 306, 714, 1028, 1065, 1108, 1990
BseYI CCCAGC 1 cut(s) 1496
BsgI GTGCAG 1 cut(s) 1941
Bsh1236I CGCG 1 cut(s) 980
BshFI GGCC 1 cut(s) 762
BsiHKAI GWGCWC 1 cut(s) 970
BslFI GGGAC 1 cut(s) 1861
BslI CCNNNNNNNGG 2 cut(s) 207, 920
BsmAI GTCTC 3 cut(s) 737, 852, 961
BsmBI CGTCTC 1 cut(s) 961
BsmFI GGGAC 1 cut(s) 1861
BsmI GAATGC 1 cut(s) 1220
BsnI GGCC 1 cut(s) 762
Bsp1286I GDGCHC 1 cut(s) 970
Bsp143I GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
Bsp1720I GCTNAGC 2 cut(s) 791, 1352
BspANI GGCC 1 cut(s) 762
BspCNI CTCAG 3 cut(s) 321, 638, 1259
BspFNI CGCG 1 cut(s) 980
BspLI GGNNCC 1 cut(s) 1620
BspMAI CTGCAG 1 cut(s) 2008
BspOI GCTAGC 2 cut(s) 1023, 1964
BspPI GGATC 3 cut(s) 343, 625, 816
BsrDI GCAATG 2 cut(s) 1548, 1790
BsrI ACTGG 4 cut(s) 21, 32, 871, 1237
BssECI CCNNGG 4 cut(s) 273, 373, 738, 1561
BssMI GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
BssNAI GTATAC 1 cut(s) 1252
BssT1I CCWWGG 4 cut(s) 273, 373, 738, 1561
Bst1107I GTATAC 1 cut(s) 1252
Bst2UI CCWGG 4 cut(s) 584, 806, 1623, 1809
Bst4CI ACNGT 3 cut(s) 231, 550, 1636
Bst6I CTCTTC 4 cut(s) 352, 476, 946, 1679
BstAPI GCANNNNNTGC 2 cut(s) 1535, 2012
BstC8I GCNNGC 5 cut(s) 172, 308, 1021, 1873, 1962
BstF5I GGATG 4 cut(s) 267, 1476, 1697, 1889
BstFNI CGCG 1 cut(s) 980
BstHHI GCGC 2 cut(s) 980, 1960
BstKTI GATC 7 cut(s) 303, 338, 505, 620, 811, 1132, 1489
BstMAI GTCTC 3 cut(s) 737, 852, 961
BstMBI GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
BstMWI GCNNNNNNNGC 7 cut(s) 197, 291, 303, 1535, 1957, 1966, 2012
BstNI CCWGG 4 cut(s) 584, 806, 1623, 1809
BstSCI CCNGG 4 cut(s) 582, 804, 1621, 1807
BstSFI CTRYAG 3 cut(s) 227, 1047, 2004
BstSLI GKGCMC 1 cut(s) 970
BstUI CGCG 1 cut(s) 980
BstV1I GCAGC 6 cut(s) 306, 714, 1028, 1065, 1108, 1990
BstV2I GAAGAC 2 cut(s) 707, 1863
BstX2I RGATCY 3 cut(s) 502, 617, 808
BstXI CCANNNNNNTGG 1 cut(s) 686
BstYI RGATCY 3 cut(s) 502, 617, 808
BstZ17I GTATAC 1 cut(s) 1252
Bsu36I CCTNAGG 1 cut(s) 205
BsuRI GGCC 1 cut(s) 762
BtsCI GGATG 4 cut(s) 267, 1476, 1697, 1889
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 3 cut(s) 68, 186, 1230
Cac8I GCNNGC 5 cut(s) 172, 308, 1021, 1873, 1962
CfoI GCGC 2 cut(s) 980, 1960
Csp6I GTAC 3 cut(s) 45, 362, 1715
CviAII CATG 2 cut(s) 129, 1966
CviQI GTAC 3 cut(s) 45, 362, 1715
DpnI GATC 7 cut(s) 302, 337, 504, 619, 810, 1131, 1488
DpnII GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
DraIII CACNNNGTG 1 cut(s) 974
DrdI GACNNNNNNGTC 1 cut(s) 1141
DseDI GACNNNNNNGTC 1 cut(s) 1141
Eam1104I CTCTTC 4 cut(s) 352, 476, 946, 1679
EarI CTCTTC 4 cut(s) 352, 476, 946, 1679
Eco130I CCWWGG 4 cut(s) 273, 373, 738, 1561
Eco57I CTGAAG 3 cut(s) 44, 1511, 1872
Eco81I CCTNAGG 1 cut(s) 205
EcoRII CCWGG 4 cut(s) 582, 804, 1621, 1807
EcoT14I CCWWGG 4 cut(s) 273, 373, 738, 1561
ErhI CCWWGG 4 cut(s) 273, 373, 738, 1561
Esp3I CGTCTC 1 cut(s) 961
FaeI CATG 2 cut(s) 132, 1969
FaqI GGGAC 1 cut(s) 1861
FatI CATG 2 cut(s) 128, 1965
FbaI TGATCA 1 cut(s) 300
FblI GTMKAC 1 cut(s) 1251
Fnu4HI GCNGC 6 cut(s) 295, 703, 1017, 1054, 1122, 2004
FokI GGATG 4 cut(s) 254, 1463, 1704, 1876
Fsp4HI GCNGC 6 cut(s) 295, 703, 1017, 1054, 1122, 2004
GlaI GCGC 2 cut(s) 979, 1959
GluI GCNGC 6 cut(s) 295, 703, 1017, 1054, 1122, 2004
GsaI CCCAGC 1 cut(s) 1500
GsuI CTGGAG 4 cut(s) 93, 852, 854, 1605
HaeIII GGCC 1 cut(s) 762
HhaI GCGC 2 cut(s) 980, 1960
Hin1II CATG 2 cut(s) 132, 1969
Hin6I GCGC 2 cut(s) 978, 1958
HinP1I GCGC 2 cut(s) 978, 1958
HincII GTYRAC 2 cut(s) 431, 540
HindII GTYRAC 2 cut(s) 431, 540
HinfI GANTC 8 cut(s) 211, 318, 367, 834, 947, 1582, 1796, 1925
HpaI GTTAAC 1 cut(s) 540
HphI GGTGA 4 cut(s) 275, 1137, 1461, 1783
Hpy166II GTNNAC 5 cut(s) 431, 540, 968, 1252, 1699
Hpy188I TCNGA 8 cut(s) 356, 628, 843, 946, 1134, 1270, 1333, 1491
Hpy188III TCNNGA 8 cut(s) 110, 719, 755, 831, 851, 1033, 1681, 1981
Hpy8I GTNNAC 5 cut(s) 431, 540, 968, 1252, 1699
HpyAV CCTTC 4 cut(s) 498, 752, 1307, 1473
HpyCH4III ACNGT 3 cut(s) 231, 550, 1636
HpyCH4IV ACGT 2 cut(s) 1001, 1281
HpyF10VI GCNNNNNNNGC 7 cut(s) 197, 291, 303, 1535, 1957, 1966, 2012
HpySE526I ACGT 2 cut(s) 1001, 1281
Hsp92II CATG 2 cut(s) 132, 1969
HspAI GCGC 2 cut(s) 978, 1958
Ksp22I TGATCA 1 cut(s) 300
KspAI GTTAAC 1 cut(s) 540
Kzo9I GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
LmnI GCTCC 1 cut(s) 1624
Lsp1109I GCAGC 6 cut(s) 306, 714, 1028, 1065, 1108, 1990
LweI GCATC 4 cut(s) 239, 1455, 1822, 1945
MaeII ACGT 2 cut(s) 1001, 1281
MaeIII GTNAC 5 cut(s) 34, 263, 389, 1143, 1656
MalI GATC 7 cut(s) 302, 337, 504, 619, 810, 1131, 1488
MboI GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
MflI RGATCY 3 cut(s) 502, 617, 808
MhlI GDGCHC 1 cut(s) 970
MlyI GAGTC 2 cut(s) 843, 1576
MmeI TCCRAC 6 cut(s) 368, 595, 840, 938, 1022, 1405
MroXI GAANNNNTTC 3 cut(s) 1171, 1462, 1676
MseI TTAA 4 cut(s) 81, 524, 539, 573
MspR9I CCNGG 4 cut(s) 584, 806, 1623, 1809
Mva1269I GAATGC 1 cut(s) 1220
MvaI CCWGG 4 cut(s) 584, 806, 1623, 1809
MvnI CGCG 1 cut(s) 980
MwoI GCNNNNNNNGC 7 cut(s) 197, 291, 303, 1535, 1957, 1966, 2012
NdeII GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
NheI GCTAGC 2 cut(s) 1019, 1960
NlaIII CATG 2 cut(s) 132, 1969
NlaIV GGNNCC 1 cut(s) 1620
NmuCI GTSAC 4 cut(s) 34, 263, 389, 1143
PcsI WCGNNNNNNNCGW 1 cut(s) 1007
PctI GAATGC 1 cut(s) 1220
PdmI GAANNNNTTC 3 cut(s) 1171, 1462, 1676
PfeI GAWTC 6 cut(s) 211, 318, 367, 947, 1796, 1925
PflMI CCANNNNNTGG 1 cut(s) 920
PfoI TCCNGGA 2 cut(s) 582, 804
PkrI GCNGC 6 cut(s) 296, 704, 1018, 1055, 1123, 2005
PleI GAGTC 2 cut(s) 842, 1576
PpsI GAGTC 2 cut(s) 842, 1576
PsiI TTATAA 1 cut(s) 1652
Psp6I CCWGG 4 cut(s) 582, 804, 1621, 1807
PspFI CCCAGC 1 cut(s) 1496
PspGI CCWGG 4 cut(s) 582, 804, 1621, 1807
PspN4I GGNNCC 1 cut(s) 1620
PstI CTGCAG 1 cut(s) 2008
PsuI RGATCY 3 cut(s) 502, 617, 808
RsaI GTAC 3 cut(s) 46, 363, 1716
RsaNI GTAC 3 cut(s) 45, 362, 1715
SaqAI TTAA 4 cut(s) 81, 524, 539, 573
SatI GCNGC 6 cut(s) 295, 703, 1017, 1054, 1122, 2004
Sau3AI GATC 7 cut(s) 300, 335, 502, 617, 808, 1129, 1486
SchI GAGTC 2 cut(s) 843, 1576
ScrFI CCNGG 4 cut(s) 584, 806, 1623, 1809
SduI GDGCHC 1 cut(s) 970
SfaNI GCATC 4 cut(s) 239, 1455, 1822, 1945
SfcI CTRYAG 3 cut(s) 227, 1047, 2004
SmlI CTYRAG 2 cut(s) 151, 289
SmoI CTYRAG 2 cut(s) 151, 289
SspI AATATT 1 cut(s) 1360
StyD4I CCNGG 4 cut(s) 582, 804, 1621, 1807
StyI CCWWGG 4 cut(s) 273, 373, 738, 1561
TaaI ACNGT 3 cut(s) 231, 550, 1636
TaiI ACGT 2 cut(s) 1004, 1284
TaqI TCGA 2 cut(s) 545, 1680
TatI WGTACW 1 cut(s) 1714
TfiI GAWTC 6 cut(s) 211, 318, 367, 947, 1796, 1925
Tru1I TTAA 4 cut(s) 81, 524, 539, 573
Tru9I TTAA 4 cut(s) 81, 524, 539, 573
TscAI CASTG 3 cut(s) 68, 193, 1237
TseFI GTSAC 4 cut(s) 34, 263, 389, 1143
TseI GCWGC 6 cut(s) 294, 702, 1016, 1053, 1121, 2003
Tsp45I GTSAC 4 cut(s) 34, 263, 389, 1143
TspDTI ATGAA 6 cut(s) 16, 435, 765, 1353, 1455, 1793
TspGWI ACGGA 1 cut(s) 379
TspRI CASTG 3 cut(s) 68, 193, 1237
Van91I CCANNNNNTGG 1 cut(s) 920
VneI GTGCAC 1 cut(s) 966
XapI RAATTY 3 cut(s) 520, 909, 1573
XbaI TCTAGA 2 cut(s) 754, 850
XcmI CCANNNNNNNNNTGG 1 cut(s) 60
XmaJI CCTAGG 1 cut(s) 1561
XmiI GTMKAC 1 cut(s) 1251
XmnI GAANNNNTTC 3 cut(s) 1171, 1462, 1676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.