RchiOBHm_Chr5g0053441

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0013021
Physical Location & Seq
Forward (+)
55843825 .. 55845436
1612 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33072

Sequence Viewer

Length: 1176 bp
ATGCAGCTCCGTCCGGCAACCACCGCCGGCGCCTTGGTCCTCTCCGTCGTCGTCTTCCTCTTCTCCGTCGCCCACGCGCACAACATCACGCGCATGCTGGCCAAGCACCCTGAGTTCTCCACCTTCAACCACTACCTAAGCTTGACCCACCTTGCCGCCGACATCAACGACCGCACCACCATAACCGTCTGCGCCGTCGACAACTCCGCCATGTCCGCCCTCCTCGCCAAGCACCTCTCCATCTACTCCATCAAGAACATCCTCTCCCTCCACGTCTTGCTCGACTACTTCGGCGCCAAGAAGCTCCACCAGATCACCAACGGCACCGCCCTCGCCGCCACCATGTTCCAGGCCACGGGCTCCGCCCCCGGCTCCGCCGGCTTCGTCAACATCACCGACCTCAAGGGCGGAAAGGTCGGATTTACCCCGAGGACAACGACGGCTCCTTCGCCGCGCATTTCCAACGTCCTCCCCTCCGCCGCCGCCGAGGCTCCGACGCCTGGCCCCGCCGAGGTCAATCTCACCGGGATCATGTCGGCCCACGGCTGCAAGGTCTTCGCCGAGACGCTCTTGGCCAACCCCGACGCGTCCAAGACCTTCAACGAAGCCGTCGACGGCGGGCTGACCGTGTTCTGCCCCGGCGACGACGCTTTCAAGGGCTTCCTGCCGAAATACAAGAACCTCTCGGCGCCCGGAAAAGCTGCATTGCTGGAGTACCATGCCGCTCCGGTGTACCAGTCCATGCCGATGTTGAAGTCCAACAATGGGTTGATGAACACTTTGGCCACCAACGGAGCGGAGAAGTACGACTTCACCGTCCAAAACGACGGTCAGGCGGTGACCCTCAAGAACAAGCTAGTGACGGCCAAGATCATCGGCACGCTAATCGATGAGCAGCCCCTTGCTATATATACTATAAACAAGGTTTTGACGCCCAAGGAGCTGTACAAGGGAGCGACTCCAACTCCGGCGCCGGCTCCGGCACCAGAGAAAGCCGCCGACGCTCCCAAGAGTTCCAAGGAGGCGCCGTCCCCCGATGACGCCGATTCCCCGGCTGACTCGCCGGAAGACGACCCGGCCGATCAGACTGCTGACGACAGTGGAGCCTTTGCGTTGGGAAGAGTGGGGTTTGGGGGTTTGATTCTCAGTGTGTGCCTTGGCTTTTCACTGTTGTAA

Protein Analysis

391

Amino Acids

40.86

Weight (kDa)

6.2

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 185 - 312 6.4e-14 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55730 AT5G55730
fragaria_vesca FvH4_3g29120
malus_domestica MD03G1179200.v1.1 MD11G1198600.v1.1
prunus_persica Prupe.4G244100_v2.0.a1
pyrus_communis pycom03g13520
rosa_chinensis RchiOBHm_Chr5g0051361 RchiOBHm_Chr5g0053441
rosa_laevigata RLG00000034881
rosa_multiflora Rmu_sc0001054.1_g000007
rosa_roxburghii Rroxscaffold_1G00027000
rosa_rugosa Rorug05G0279900
rosa_samantha Rh5AG351000 Rh5BG363300 Rh5CG387700 Rh5DG377900
rosa_wichuraiana Rw5G033200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 815
AccB1I GGYRCC 7 cut(s) 29, 293, 323, 688, 970, 982, 1024
AccBSI CCGCTC 2 cut(s) 725, 797
AccI GTMKAC 2 cut(s) 198, 612
AccII CGCG 4 cut(s) 77, 91, 454, 587
AclWI GGATC 1 cut(s) 536
AcoI YGGCCR 5 cut(s) 99, 573, 783, 864, 1077
AcyI GRCGYC 8 cut(s) 30, 294, 497, 689, 932, 971, 1025, 1041
AfaI GTAC 4 cut(s) 716, 734, 806, 947
AfiI CCNNNNNNNGG 4 cut(s) 355, 500, 511, 765
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 4 cut(s) 127, 601, 655, 754
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 2 cut(s) 348, 499
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 7, 141, 304, 701, 856, 943
AluI AGCT 6 cut(s) 7, 141, 304, 701, 856, 943
Alw26I GTCTC 1 cut(s) 557
AlwI GGATC 1 cut(s) 536
Ama87I CYCGRG 1 cut(s) 427
AoxI GGCC 8 cut(s) 99, 351, 502, 537, 573, 783, 864, 1077
ApeKI GCWGC 4 cut(s) 4, 546, 701, 895
ArsI GACNNNNNNTTYG 4 cut(s) 430, 462, 814, 846
AspLEI GCGC 9 cut(s) 32, 79, 93, 194, 296, 456, 691, 973, 1027
AspS9I GGNCC 3 cut(s) 37, 503, 538
AsuC2I CCSGG 6 cut(s) 369, 526, 639, 693, 1052, 1076
AsuHPI GGTGA 5 cut(s) 307, 385, 514, 805, 850
AvaI CYCGRG 1 cut(s) 427
AvaII GGWCC 1 cut(s) 37
BalI TGGCCA 3 cut(s) 101, 575, 785
BanI GGYRCC 7 cut(s) 29, 293, 323, 688, 970, 982, 1024
BanII GRGCYC 1 cut(s) 362
BbsI GAAGAC 3 cut(s) 46, 547, 1074
BbvI GCAGC 4 cut(s) 16, 533, 688, 907
BccI CCATC 2 cut(s) 248, 257
BceAI ACGGC 8 cut(s) 179, 337, 456, 559, 593, 631, 879, 1012
BcgI CGANNNNNNTGC 8 cut(s) 313, 347, 538, 572, 868, 902, 1070, 1104
BciT130I CCWGG 2 cut(s) 350, 501
BcnI CCSGG 6 cut(s) 369, 526, 639, 693, 1052, 1076
BcoDI GTCTC 1 cut(s) 557
BfaI CTAG 1 cut(s) 857
BfoI RGCGCY 5 cut(s) 33, 297, 692, 974, 1028
BglI GCCNNNNNGGC 1 cut(s) 488
Bme1390I CCNGG 8 cut(s) 350, 369, 501, 526, 639, 693, 1052, 1076
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 427
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 3 cut(s) 37, 503, 538
BmrFI CCNGG 8 cut(s) 350, 369, 501, 526, 639, 693, 1052, 1076
BpiI GAAGAC 3 cut(s) 46, 547, 1074
BpmI CTGGAG 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 386, 830
BpuMI CCSGG 6 cut(s) 369, 526, 639, 693, 1052, 1076
Bsa29I ATCGAT 1 cut(s) 888
BsaHI GRCGYC 8 cut(s) 30, 294, 497, 689, 932, 971, 1025, 1041
BsaWI WCCGGW 1 cut(s) 727
BsaXI ACNNNNNCTCC 8 cut(s) 248, 278, 427, 457, 949, 979, 1013, 1043
Bsc4I CCNNNNNNNGG 4 cut(s) 355, 500, 511, 765
Bse118I RCCGGY 3 cut(s) 26, 377, 973
Bse1I ACTGG 1 cut(s) 736
Bse3DI GCAATG 1 cut(s) 704
BseBI CCWGG 2 cut(s) 350, 501
BseCI ATCGAT 1 cut(s) 888
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 4 cut(s) 355, 500, 511, 765
BseMI GCAATG 1 cut(s) 704
BseMII CTCAG 2 cut(s) 102, 1159
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 1 cut(s) 212
BseX3I CGGCCG 1 cut(s) 1077
BseXI GCAGC 4 cut(s) 16, 533, 688, 907
Bsh1236I CGCG 4 cut(s) 77, 91, 454, 587
Bsh1285I CGRYCG 2 cut(s) 172, 1080
BshFI GGCC 8 cut(s) 101, 353, 504, 539, 575, 785, 866, 1079
BshNI GGYRCC 7 cut(s) 29, 293, 323, 688, 970, 982, 1024
BshVI ATCGAT 1 cut(s) 888
BsiEI CGRYCG 2 cut(s) 172, 1080
BsiHKCI CYCGRG 1 cut(s) 427
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 4 cut(s) 355, 500, 511, 765
BsmAI GTCTC 1 cut(s) 557
BsmBI CGTCTC 1 cut(s) 557
BsmFI GGGAC 1 cut(s) 1015
BsnI GGCC 8 cut(s) 101, 353, 504, 539, 575, 785, 866, 1079
BsoBI CYCGRG 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 362
Bsp1407I TGTACA 1 cut(s) 945
Bsp143I GATC 4 cut(s) 312, 528, 870, 1081
BspANI GGCC 8 cut(s) 101, 353, 504, 539, 575, 785, 866, 1079
BspCNI CTCAG 2 cut(s) 103, 1158
BspDI ATCGAT 1 cut(s) 888
BspFNI CGCG 4 cut(s) 77, 91, 454, 587
BspPI GGATC 1 cut(s) 536
BspT107I GGYRCC 7 cut(s) 29, 293, 323, 688, 970, 982, 1024
BsrBI CCGCTC 2 cut(s) 725, 797
BsrDI GCAATG 1 cut(s) 704
BsrFI RCCGGY 3 cut(s) 26, 377, 973
BsrGI TGTACA 1 cut(s) 945
BsrI ACTGG 1 cut(s) 736
BssAI RCCGGY 3 cut(s) 26, 377, 973
BssMI GATC 4 cut(s) 312, 528, 870, 1081
BssNI GRCGYC 8 cut(s) 30, 294, 497, 689, 932, 971, 1025, 1041
BssT1I CCWWGG 4 cut(s) 33, 936, 1017, 1156
Bst2UI CCWGG 2 cut(s) 350, 501
Bst4CI ACNGT 6 cut(s) 187, 628, 817, 830, 1100, 1170
Bst6I CTCTTC 2 cut(s) 65, 1114
BstACI GRCGYC 8 cut(s) 30, 294, 497, 689, 932, 971, 1025, 1041
BstAUI TGTACA 1 cut(s) 945
BstC8I GCNNGC 7 cut(s) 28, 95, 99, 379, 620, 881, 975
BstDEI CTNAG 3 cut(s) 111, 137, 1145
BstDSI CCRYGG 2 cut(s) 354, 541
BstEII GGTNACC 1 cut(s) 838
BstF5I GGATG 1 cut(s) 258
BstFNI CGCG 4 cut(s) 77, 91, 454, 587
BstH2I RGCGCY 5 cut(s) 33, 297, 692, 974, 1028
BstHHI GCGC 9 cut(s) 32, 79, 93, 194, 296, 456, 691, 973, 1027
BstKTI GATC 4 cut(s) 315, 531, 873, 1084
BstMAI GTCTC 1 cut(s) 557
BstMBI GATC 4 cut(s) 312, 528, 870, 1081
BstMCI CGRYCG 2 cut(s) 172, 1080
BstMWI GCNNNNNNNGC 9 cut(s) 23, 103, 215, 224, 335, 378, 488, 940, 1001
BstNI CCWGG 2 cut(s) 350, 501
BstNSI RCATGY 1 cut(s) 97
BstPI GGTNACC 1 cut(s) 838
BstSCI CCNGG 8 cut(s) 348, 367, 499, 524, 637, 691, 1050, 1074
BstUI CGCG 4 cut(s) 77, 91, 454, 587
BstV1I GCAGC 4 cut(s) 16, 533, 688, 907
BstV2I GAAGAC 3 cut(s) 46, 547, 1074
BstZI CGGCCG 1 cut(s) 1077
Bsu15I ATCGAT 1 cut(s) 888
BsuRI GGCC 8 cut(s) 101, 353, 504, 539, 575, 785, 866, 1079
BsuTUI ATCGAT 1 cut(s) 888
BtgI CCRYGG 2 cut(s) 354, 541
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 1 cut(s) 258
BtsIMutI CAGTG 3 cut(s) 1105, 1153, 1166
Cac8I GCNNGC 7 cut(s) 28, 95, 99, 379, 620, 881, 975
CfoI GCGC 9 cut(s) 32, 79, 93, 194, 296, 456, 691, 973, 1027
Cfr10I RCCGGY 3 cut(s) 26, 377, 973
Cfr13I GGNCC 3 cut(s) 37, 503, 538
ClaI ATCGAT 1 cut(s) 888
CseI GACGC 8 cut(s) 505, 574, 576, 593, 656, 940, 1010, 1049
Csp6I GTAC 4 cut(s) 715, 733, 805, 946
CviAII CATG 6 cut(s) 94, 211, 343, 532, 719, 742
CviQI GTAC 4 cut(s) 715, 733, 805, 946
DdeI CTNAG 3 cut(s) 111, 137, 1145
DinI GGCGCC 5 cut(s) 31, 295, 690, 972, 1026
DpnI GATC 4 cut(s) 314, 530, 872, 1083
DpnII GATC 4 cut(s) 312, 528, 870, 1081
DrdI GACNNNNNNGTC 1 cut(s) 815
DseDI GACNNNNNNGTC 1 cut(s) 815
EaeI YGGCCR 5 cut(s) 99, 573, 783, 864, 1077
EagI CGGCCG 1 cut(s) 1077
Eam1104I CTCTTC 2 cut(s) 65, 1114
EarI CTCTTC 2 cut(s) 65, 1114
EciI GGCGGA 6 cut(s) 196, 205, 352, 364, 423, 466
EclXI CGGCCG 1 cut(s) 1077
Eco130I CCWWGG 4 cut(s) 33, 936, 1017, 1156
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 37
Eco52I CGGCCG 1 cut(s) 1077
Eco88I CYCGRG 1 cut(s) 427
Eco91I GGTNACC 1 cut(s) 838
EcoO65I GGTNACC 1 cut(s) 838
EcoRII CCWGG 2 cut(s) 348, 499
EcoT14I CCWWGG 4 cut(s) 33, 936, 1017, 1156
EcoT38I GRGCYC 1 cut(s) 362
EgeI GGCGCC 5 cut(s) 31, 295, 690, 972, 1026
EheI GGCGCC 5 cut(s) 31, 295, 690, 972, 1026
ErhI CCWWGG 4 cut(s) 33, 936, 1017, 1156
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 6 cut(s) 97, 214, 346, 535, 722, 745
FalI AAGNNNNNCTT 2 cut(s) 794, 826
FaqI GGGAC 1 cut(s) 1015
FatI CATG 6 cut(s) 93, 210, 342, 531, 718, 741
FauI CCCGC 2 cut(s) 514, 611
FblI GTMKAC 2 cut(s) 198, 612
FokI GGATG 1 cut(s) 245
FriOI GRGCYC 1 cut(s) 362
FspBI CTAG 1 cut(s) 857
GlaI GCGC 9 cut(s) 31, 78, 92, 193, 295, 455, 690, 972, 1026
GsuI CTGGAG 1 cut(s) 731
HaeII RGCGCY 5 cut(s) 33, 297, 692, 974, 1028
HaeIII GGCC 8 cut(s) 101, 353, 504, 539, 575, 785, 866, 1079
HgaI GACGC 8 cut(s) 505, 574, 576, 593, 656, 940, 1010, 1049
HhaI GCGC 9 cut(s) 32, 79, 93, 194, 296, 456, 691, 973, 1027
Hin1I GRCGYC 8 cut(s) 30, 294, 497, 689, 932, 971, 1025, 1041
Hin1II CATG 6 cut(s) 97, 214, 346, 535, 722, 745
Hin6I GCGC 9 cut(s) 30, 77, 91, 192, 294, 454, 689, 971, 1025
HinP1I GCGC 9 cut(s) 30, 77, 91, 192, 294, 454, 689, 971, 1025
HincII GTYRAC 3 cut(s) 199, 388, 613
HindII GTYRAC 3 cut(s) 199, 388, 613
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 4 cut(s) 958, 1046, 1058, 1141
HphI GGTGA 5 cut(s) 307, 385, 514, 805, 850
Hpy166II GTNNAC 4 cut(s) 199, 388, 613, 733
Hpy188I TCNGA 3 cut(s) 419, 495, 1086
Hpy188III TCNNGA 2 cut(s) 253, 847
Hpy8I GTNNAC 4 cut(s) 199, 388, 613, 733
HpyAV CCTTC 3 cut(s) 133, 456, 607
HpyCH4III ACNGT 6 cut(s) 187, 628, 817, 830, 1100, 1170
HpyCH4IV ACGT 2 cut(s) 273, 465
HpyCH4V TGCA 3 cut(s) 4, 549, 704
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 103, 215, 224, 335, 378, 488, 940, 1001
HpyF3I CTNAG 3 cut(s) 111, 137, 1145
HpySE526I ACGT 2 cut(s) 273, 465
Hsp92I GRCGYC 8 cut(s) 30, 294, 497, 689, 932, 971, 1025, 1041
Hsp92II CATG 6 cut(s) 97, 214, 346, 535, 722, 745
HspAI GCGC 9 cut(s) 30, 77, 91, 192, 294, 454, 689, 971, 1025
KasI GGCGCC 5 cut(s) 29, 293, 688, 970, 1024
KroI GCCGGC 3 cut(s) 26, 377, 973
KroNI GCCGGC 3 cut(s) 28, 379, 975
Kzo9I GATC 4 cut(s) 312, 528, 870, 1081
Lsp1109I GCAGC 4 cut(s) 16, 533, 688, 907
MaeI CTAG 1 cut(s) 857
MaeII ACGT 2 cut(s) 273, 465
MaeIII GTNAC 2 cut(s) 838, 859
MalI GATC 4 cut(s) 314, 530, 872, 1083
MbiI CCGCTC 2 cut(s) 725, 797
MboI GATC 4 cut(s) 312, 528, 870, 1081
MboII GAAGA 5 cut(s) 46, 52, 547, 1079, 1131
MhlI GDGCHC 1 cut(s) 362
MlsI TGGCCA 3 cut(s) 101, 575, 785
MluI ACGCGT 1 cut(s) 585
MluNI TGGCCA 3 cut(s) 101, 575, 785
Mly113I GGCGCC 5 cut(s) 30, 294, 689, 971, 1025
MlyI GAGTC 2 cut(s) 952, 1052
MmeI TCCRAC 5 cut(s) 397, 486, 518, 783, 986
Mox20I TGGCCA 3 cut(s) 101, 575, 785
MreI CGCCGGCG 1 cut(s) 26
MroNI GCCGGC 3 cut(s) 26, 377, 973
MscI TGGCCA 3 cut(s) 101, 575, 785
MslI CAYNNNNRTG 2 cut(s) 92, 746
Msp20I TGGCCA 3 cut(s) 101, 575, 785
MspR9I CCNGG 8 cut(s) 350, 369, 501, 526, 639, 693, 1052, 1076
MvaI CCWGG 2 cut(s) 350, 501
MvnI CGCG 4 cut(s) 77, 91, 454, 587
MwoI GCNNNNNNNGC 9 cut(s) 23, 103, 215, 224, 335, 378, 488, 940, 1001
NaeI GCCGGC 3 cut(s) 28, 379, 975
NarI GGCGCC 5 cut(s) 30, 294, 689, 971, 1025
NciI CCSGG 6 cut(s) 369, 526, 639, 693, 1052, 1076
NdeII GATC 4 cut(s) 312, 528, 870, 1081
NgoMIV GCCGGC 3 cut(s) 26, 377, 973
NlaIII CATG 6 cut(s) 97, 214, 346, 535, 722, 745
NmeAIII GCCGAG 4 cut(s) 511, 535, 586, 665
NmuCI GTSAC 2 cut(s) 838, 859
NspI RCATGY 1 cut(s) 97
PaeI GCATGC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 609, 813, 1077
PdiI GCCGGC 3 cut(s) 28, 379, 975
PfeI GAWTC 2 cut(s) 1046, 1141
PleI GAGTC 2 cut(s) 952, 1052
PluTI GGCGCC 5 cut(s) 33, 297, 692, 974, 1028
PpsI GAGTC 2 cut(s) 952, 1052
Psp6I CCWGG 2 cut(s) 348, 499
PspEI GGTNACC 1 cut(s) 838
PspGI CCWGG 2 cut(s) 348, 499
PspPI GGNCC 3 cut(s) 37, 503, 538
RsaI GTAC 4 cut(s) 716, 734, 806, 947
RsaNI GTAC 4 cut(s) 715, 733, 805, 946
RseI CAYNNNNRTG 2 cut(s) 92, 746
SalI GTCGAC 2 cut(s) 197, 611
Sau3AI GATC 4 cut(s) 312, 528, 870, 1081
Sau96I GGNCC 3 cut(s) 37, 503, 538
SchI GAGTC 2 cut(s) 952, 1052
ScrFI CCNGG 8 cut(s) 350, 369, 501, 526, 639, 693, 1052, 1076
SduI GDGCHC 1 cut(s) 362
SfoI GGCGCC 5 cut(s) 31, 295, 690, 972, 1026
SgrAI CRCCGGYG 1 cut(s) 26
SgrDI CGTCGACG 1 cut(s) 611
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 92, 746
SmlI CTYRAG 2 cut(s) 401, 845
SmoI CTYRAG 2 cut(s) 401, 845
SphI GCATGC 1 cut(s) 97
SspDI GGCGCC 5 cut(s) 29, 293, 688, 970, 1024
SspMI CTAG 1 cut(s) 857
StyD4I CCNGG 8 cut(s) 348, 367, 499, 524, 637, 691, 1050, 1074
StyI CCWWGG 4 cut(s) 33, 936, 1017, 1156
TaaI ACNGT 6 cut(s) 187, 628, 817, 830, 1100, 1170
TaiI ACGT 2 cut(s) 276, 468
TaqI TCGA 4 cut(s) 198, 282, 612, 888
TatI WGTACW 1 cut(s) 945
TauI GCSGC 7 cut(s) 158, 338, 454, 482, 485, 725, 998
TfiI GAWTC 2 cut(s) 1046, 1141
TscAI CASTG 3 cut(s) 1105, 1153, 1173
TseFI GTSAC 2 cut(s) 838, 859
TseI GCWGC 4 cut(s) 4, 546, 701, 895
Tsp45I GTSAC 2 cut(s) 838, 859
TspDTI ATGAA 1 cut(s) 788
TspGWI ACGGA 3 cut(s) 34, 55, 807
TspRI CASTG 3 cut(s) 1105, 1153, 1173
VpaK11BI GGWCC 1 cut(s) 37
XceI RCATGY 1 cut(s) 97
XmiI GTMKAC 2 cut(s) 198, 612
XspI CTAG 1 cut(s) 857
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.