Rw5G033200

Fasciclin-like arabinogalactan protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0013021
Physical Location & Seq
Forward (+)
56335998 .. 56337623
1626 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G033200.1

Sequence Viewer

Length: 1227 bp
ATGCAGCTCCGTCCGGCAACCACCGCCGGCGCCTTGGTCCTCTCCGTCGTCGTCTTCCTCTTCTCCGTCGCCCACGCTCACAACATCACGCGCATGCTGGCCAAGCACCCTGAGTTCTCCACCTTCAACCACTACCTAAGCTTGACCCACCTTGCCGCCGACATCAACGACCGCACCACCATAACCGTCTGCGCCGTCGACAACTCCGCCATGTCCGCCCTCCTCGCCAAGCACCTCTCCATCTACTCCATCAAGAACATCCTCTCCCTCCACGTCTTGCTCGACTACTTCGGCGCCAAGAAGCTCCACCAGATCACCAACGGCACCGCCCTCGCCGCCACCATGTTCCAGGCCACCGGCTCCGCCCCCGGCTCCTCCGGCTTCGTCAACATCACCGACCTCAAGGGCGGAAAGGTCGGATTTACCCCCGAGGACAACGACGGCTCCTTCGCCGCGCATTTCGTCAAGTCCGTCGACGAACTCCCCTACAACATCTCCGTCATCCAAATCAGCAACGTCCTCCCCTCCGCCGCCGCCGAGGCTCCGACGCCTGGCCCCGCCGAGGTCAATCTCACCGGGATCATGTCGGCCCACGGCTGCAAGGTCTTCGCCGAGACGCTCTTGGCCAACCCCGACGCGTCCAAGACCTTCGACGAAGCCGTCGACGGCGGGCTGACCGTGTTCTGCCCCGGCGACGACGCTTTCAAGGGCTTCCTGCCGAAATACAAGAACCTCTCGGCGCCCGGAAAAGCTGCATTGCTGGAGTACCACGCCGCTCCGGTGTACCAGTCCATGCCGATGTTGAAGTCCAACAATGGGTTGATGAACACTTTGGCCACCAACGGAGCGGAGAAGTACGACTTCACCGTCCAAAACGACGGTCAGGCGGTGACCCTCAAGAACAAGCTAGTGACGGCCAAGATCATCGGCACGCTTATCGATGAGCAGCCCCTTGCTATATACACTATAAACAAGGTTTTGACGCCCAAGGAGCTGTACAAGGGAGAGGCTCCAACTCCGGCGCCGGCTCCGGCACCAGAGAAAGCCGCCGACGCGCCCAAGAGTTCCAAGAAGGCGCCGTCCCCGGACGACGCCGATTCCCCGGCTGACTCGCCGGAAGACGACCCAGCCGATCAGACTGCTGACGACAGTGGAGCCTTTGCTTTGGGAAGAGTGGGTTTTGGGGGTTTGATTCTCAGTGTGTGCCTTGGCTTTTCACTGTTGTAA

Protein Analysis

408

Amino Acids

42.82

Weight (kDa)

5.53

Isoelectric Point (pI)

33.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Fasciclin PF02469 203 - 329 1.4e-13 Fasciclin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013021)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G55730 AT5G55730
fragaria_vesca FvH4_3g29120
malus_domestica MD03G1179200.v1.1 MD11G1198600.v1.1
prunus_persica Prupe.4G244100_v2.0.a1
pyrus_communis pycom03g13520
rosa_chinensis RchiOBHm_Chr5g0051361 RchiOBHm_Chr5g0053441
rosa_laevigata RLG00000034881
rosa_multiflora Rmu_sc0001054.1_g000007
rosa_roxburghii Rroxscaffold_1G00027000
rosa_rugosa Rorug05G0279900
rosa_samantha Rh5AG351000 Rh5BG363300 Rh5CG387700 Rh5DG377900
rosa_wichuraiana Rw5G033200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 659, 866
AccB1I GGYRCC 7 cut(s) 29, 293, 323, 739, 1021, 1033, 1075
AccBSI CCGCTC 2 cut(s) 776, 848
AccI GTMKAC 3 cut(s) 198, 474, 663
AccII CGCG 4 cut(s) 91, 455, 638, 1055
AclWI GGATC 1 cut(s) 587
AcoI YGGCCR 4 cut(s) 99, 624, 834, 915
AcyI GRCGYC 8 cut(s) 30, 294, 548, 740, 983, 1022, 1076, 1092
AfaI GTAC 4 cut(s) 767, 785, 857, 998
AfiI CCNNNNNNNGG 3 cut(s) 551, 562, 816
AflIII ACRYGT 1 cut(s) 636
AgsI TTSAA 3 cut(s) 127, 706, 805
AjiI CACGTC 1 cut(s) 274
AjnI CCWGG 2 cut(s) 348, 550
AloI GAACNNNNNNTCC 2 cut(s) 248, 280
AluBI AGCT 6 cut(s) 7, 141, 304, 752, 907, 994
AluI AGCT 6 cut(s) 7, 141, 304, 752, 907, 994
Alw26I GTCTC 1 cut(s) 608
AlwI GGATC 1 cut(s) 587
Ama87I CYCGRG 1 cut(s) 428
AoxI GGCC 7 cut(s) 99, 351, 553, 588, 624, 834, 915
ApeKI GCWGC 4 cut(s) 4, 597, 752, 946
ArsI GACNNNNNNTTYG 4 cut(s) 431, 463, 865, 897
AspLEI GCGC 9 cut(s) 32, 93, 194, 296, 457, 742, 1024, 1057, 1078
AspS9I GGNCC 3 cut(s) 37, 554, 589
AsuC2I CCSGG 6 cut(s) 369, 577, 690, 744, 1085, 1103
AsuHPI GGTGA 5 cut(s) 307, 385, 565, 856, 901
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 1 cut(s) 37
BalI TGGCCA 3 cut(s) 101, 626, 836
BanI GGYRCC 7 cut(s) 29, 293, 323, 739, 1021, 1033, 1075
BbsI GAAGAC 3 cut(s) 46, 598, 1125
BbvI GCAGC 4 cut(s) 16, 584, 739, 958
BccI CCATC 2 cut(s) 248, 257
BceAI ACGGC 8 cut(s) 179, 337, 457, 610, 644, 682, 930, 1063
BcgI CGANNNNNNTGC 8 cut(s) 313, 347, 589, 623, 919, 953, 1121, 1155
BciT130I CCWGG 2 cut(s) 350, 552
BcnI CCSGG 6 cut(s) 369, 577, 690, 744, 1085, 1103
BcoDI GTCTC 1 cut(s) 608
BfaI CTAG 1 cut(s) 908
BfoI RGCGCY 5 cut(s) 33, 297, 743, 1025, 1079
BglI GCCNNNNNGGC 1 cut(s) 539
Bme1390I CCNGG 8 cut(s) 350, 369, 552, 577, 690, 744, 1085, 1103
Bme18I GGWCC 1 cut(s) 37
BmeT110I CYCGRG 1 cut(s) 428
BmgBI CACGTC 1 cut(s) 274
BmgT120I GGNCC 3 cut(s) 37, 554, 589
BmrFI CCNGG 8 cut(s) 350, 369, 552, 577, 690, 744, 1085, 1103
BpiI GAAGAC 3 cut(s) 46, 598, 1125
BpmI CTGGAG 1 cut(s) 782
Bpu10I CCTNAGC 1 cut(s) 137
BpuEI CTTGAG 2 cut(s) 386, 881
BpuMI CCSGG 6 cut(s) 369, 577, 690, 744, 1085, 1103
Bsa29I ATCGAT 1 cut(s) 939
BsaHI GRCGYC 8 cut(s) 30, 294, 548, 740, 983, 1022, 1076, 1092
BsaWI WCCGGW 1 cut(s) 778
BsaXI ACNNNNNCTCC 6 cut(s) 248, 278, 428, 458, 479, 509
Bsc4I CCNNNNNNNGG 3 cut(s) 551, 562, 816
Bse118I RCCGGY 3 cut(s) 26, 356, 1024
Bse1I ACTGG 1 cut(s) 787
Bse3DI GCAATG 1 cut(s) 755
BseBI CCWGG 2 cut(s) 350, 552
BseCI ATCGAT 1 cut(s) 939
BseGI GGATG 2 cut(s) 258, 501
BseLI CCNNNNNNNGG 3 cut(s) 551, 562, 816
BseMI GCAATG 1 cut(s) 755
BseMII CTCAG 2 cut(s) 102, 1210
BseNI ACTGG 1 cut(s) 787
BseRI GAGGAG 2 cut(s) 212, 364
BseXI GCAGC 4 cut(s) 16, 584, 739, 958
BseYI CCCAGC 1 cut(s) 1126
Bsh1236I CGCG 4 cut(s) 91, 455, 638, 1055
Bsh1285I CGRYCG 1 cut(s) 172
BshFI GGCC 7 cut(s) 101, 353, 555, 590, 626, 836, 917
BshNI GGYRCC 7 cut(s) 29, 293, 323, 739, 1021, 1033, 1075
BshVI ATCGAT 1 cut(s) 939
BsiEI CGRYCG 1 cut(s) 172
BsiHKCI CYCGRG 1 cut(s) 428
BslFI GGGAC 1 cut(s) 1066
BslI CCNNNNNNNGG 3 cut(s) 551, 562, 816
BsmAI GTCTC 1 cut(s) 608
BsmBI CGTCTC 1 cut(s) 608
BsmFI GGGAC 1 cut(s) 1066
BsnI GGCC 7 cut(s) 101, 353, 555, 590, 626, 836, 917
BsoBI CYCGRG 1 cut(s) 428
Bsp1407I TGTACA 1 cut(s) 996
Bsp143I GATC 4 cut(s) 312, 579, 921, 1132
BspANI GGCC 7 cut(s) 101, 353, 555, 590, 626, 836, 917
BspCNI CTCAG 2 cut(s) 103, 1209
BspDI ATCGAT 1 cut(s) 939
BspFNI CGCG 4 cut(s) 91, 455, 638, 1055
BspPI GGATC 1 cut(s) 587
BspT107I GGYRCC 7 cut(s) 29, 293, 323, 739, 1021, 1033, 1075
BsrBI CCGCTC 2 cut(s) 776, 848
BsrDI GCAATG 1 cut(s) 755
BsrFI RCCGGY 3 cut(s) 26, 356, 1024
BsrGI TGTACA 1 cut(s) 996
BsrI ACTGG 1 cut(s) 787
BssAI RCCGGY 3 cut(s) 26, 356, 1024
BssMI GATC 4 cut(s) 312, 579, 921, 1132
BssNI GRCGYC 8 cut(s) 30, 294, 548, 740, 983, 1022, 1076, 1092
BssT1I CCWWGG 3 cut(s) 33, 987, 1207
Bst2UI CCWGG 2 cut(s) 350, 552
Bst4CI ACNGT 6 cut(s) 187, 679, 868, 881, 1151, 1221
Bst6I CTCTTC 2 cut(s) 65, 1165
BstACI GRCGYC 8 cut(s) 30, 294, 548, 740, 983, 1022, 1076, 1092
BstAUI TGTACA 1 cut(s) 996
BstC8I GCNNGC 6 cut(s) 28, 95, 99, 671, 932, 1026
BstDEI CTNAG 3 cut(s) 111, 137, 1196
BstDSI CCRYGG 1 cut(s) 592
BstEII GGTNACC 1 cut(s) 889
BstF5I GGATG 2 cut(s) 258, 501
BstFNI CGCG 4 cut(s) 91, 455, 638, 1055
BstH2I RGCGCY 5 cut(s) 33, 297, 743, 1025, 1079
BstHHI GCGC 9 cut(s) 32, 93, 194, 296, 457, 742, 1024, 1057, 1078
BstKTI GATC 4 cut(s) 315, 582, 924, 1135
BstMAI GTCTC 1 cut(s) 608
BstMBI GATC 4 cut(s) 312, 579, 921, 1132
BstMCI CGRYCG 1 cut(s) 172
BstMWI GCNNNNNNNGC 9 cut(s) 23, 103, 215, 224, 335, 378, 539, 991, 1052
BstNI CCWGG 2 cut(s) 350, 552
BstNSI RCATGY 1 cut(s) 97
BstPI GGTNACC 1 cut(s) 889
BstSCI CCNGG 8 cut(s) 348, 367, 550, 575, 688, 742, 1083, 1101
BstUI CGCG 4 cut(s) 91, 455, 638, 1055
BstV1I GCAGC 4 cut(s) 16, 584, 739, 958
BstV2I GAAGAC 3 cut(s) 46, 598, 1125
Bsu15I ATCGAT 1 cut(s) 939
BsuRI GGCC 7 cut(s) 101, 353, 555, 590, 626, 836, 917
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 1 cut(s) 592
BtrI CACGTC 1 cut(s) 274
BtsCI GGATG 2 cut(s) 258, 501
BtsIMutI CAGTG 3 cut(s) 1156, 1204, 1217
Cac8I GCNNGC 6 cut(s) 28, 95, 99, 671, 932, 1026
CfoI GCGC 9 cut(s) 32, 93, 194, 296, 457, 742, 1024, 1057, 1078
Cfr10I RCCGGY 3 cut(s) 26, 356, 1024
Cfr13I GGNCC 3 cut(s) 37, 554, 589
ClaI ATCGAT 1 cut(s) 939
CseI GACGC 8 cut(s) 556, 625, 627, 644, 707, 991, 1061, 1100
Csp6I GTAC 4 cut(s) 766, 784, 856, 997
CviAII CATG 5 cut(s) 94, 211, 343, 583, 793
CviQI GTAC 4 cut(s) 766, 784, 856, 997
DdeI CTNAG 3 cut(s) 111, 137, 1196
DinI GGCGCC 5 cut(s) 31, 295, 741, 1023, 1077
DpnI GATC 4 cut(s) 314, 581, 923, 1134
DpnII GATC 4 cut(s) 312, 579, 921, 1132
DrdI GACNNNNNNGTC 2 cut(s) 659, 866
DseDI GACNNNNNNGTC 2 cut(s) 659, 866
EaeI YGGCCR 4 cut(s) 99, 624, 834, 915
Eam1104I CTCTTC 2 cut(s) 65, 1165
EarI CTCTTC 2 cut(s) 65, 1165
EciI GGCGGA 5 cut(s) 196, 205, 352, 423, 517
Eco130I CCWWGG 3 cut(s) 33, 987, 1207
Eco47I GGWCC 1 cut(s) 37
Eco88I CYCGRG 1 cut(s) 428
Eco91I GGTNACC 1 cut(s) 889
EcoO65I GGTNACC 1 cut(s) 889
EcoRII CCWGG 2 cut(s) 348, 550
EcoT14I CCWWGG 3 cut(s) 33, 987, 1207
EgeI GGCGCC 5 cut(s) 31, 295, 741, 1023, 1077
EheI GGCGCC 5 cut(s) 31, 295, 741, 1023, 1077
ErhI CCWWGG 3 cut(s) 33, 987, 1207
Esp3I CGTCTC 1 cut(s) 608
FaeI CATG 5 cut(s) 97, 214, 346, 586, 796
FaiI YATR 9 cut(s) 95, 182, 212, 344, 584, 794, 959, 961, 968
FalI AAGNNNNNCTT 2 cut(s) 845, 877
FaqI GGGAC 1 cut(s) 1066
FatI CATG 5 cut(s) 93, 210, 342, 582, 792
FauI CCCGC 2 cut(s) 565, 662
FblI GTMKAC 3 cut(s) 198, 474, 663
FokI GGATG 2 cut(s) 245, 488
FspBI CTAG 1 cut(s) 908
GlaI GCGC 9 cut(s) 31, 92, 193, 295, 456, 741, 1023, 1056, 1077
GsaI CCCAGC 1 cut(s) 1130
GsuI CTGGAG 1 cut(s) 782
HaeII RGCGCY 5 cut(s) 33, 297, 743, 1025, 1079
HaeIII GGCC 7 cut(s) 101, 353, 555, 590, 626, 836, 917
HgaI GACGC 8 cut(s) 556, 625, 627, 644, 707, 991, 1061, 1100
HhaI GCGC 9 cut(s) 32, 93, 194, 296, 457, 742, 1024, 1057, 1078
Hin1I GRCGYC 8 cut(s) 30, 294, 548, 740, 983, 1022, 1076, 1092
Hin1II CATG 5 cut(s) 97, 214, 346, 586, 796
Hin6I GCGC 9 cut(s) 30, 91, 192, 294, 455, 740, 1022, 1055, 1076
HinP1I GCGC 9 cut(s) 30, 91, 192, 294, 455, 740, 1022, 1055, 1076
HincII GTYRAC 4 cut(s) 199, 388, 475, 664
HindII GTYRAC 4 cut(s) 199, 388, 475, 664
HindIII AAGCTT 1 cut(s) 139
HinfI GANTC 3 cut(s) 1097, 1109, 1192
HphI GGTGA 5 cut(s) 307, 385, 565, 856, 901
Hpy166II GTNNAC 5 cut(s) 199, 388, 475, 664, 784
Hpy188I TCNGA 3 cut(s) 419, 546, 1137
Hpy188III TCNNGA 2 cut(s) 253, 898
Hpy8I GTNNAC 5 cut(s) 199, 388, 475, 664, 784
HpyAV CCTTC 4 cut(s) 133, 457, 658, 1066
HpyCH4III ACNGT 6 cut(s) 187, 679, 868, 881, 1151, 1221
HpyCH4IV ACGT 2 cut(s) 273, 516
HpyCH4V TGCA 3 cut(s) 4, 600, 755
HpyF10VI GCNNNNNNNGC 9 cut(s) 23, 103, 215, 224, 335, 378, 539, 991, 1052
HpyF3I CTNAG 3 cut(s) 111, 137, 1196
HpySE526I ACGT 2 cut(s) 273, 516
Hsp92I GRCGYC 8 cut(s) 30, 294, 548, 740, 983, 1022, 1076, 1092
Hsp92II CATG 5 cut(s) 97, 214, 346, 586, 796
HspAI GCGC 9 cut(s) 30, 91, 192, 294, 455, 740, 1022, 1055, 1076
KasI GGCGCC 5 cut(s) 29, 293, 739, 1021, 1075
KroI GCCGGC 2 cut(s) 26, 1024
KroNI GCCGGC 2 cut(s) 28, 1026
Kzo9I GATC 4 cut(s) 312, 579, 921, 1132
Lsp1109I GCAGC 4 cut(s) 16, 584, 739, 958
MaeI CTAG 1 cut(s) 908
MaeII ACGT 2 cut(s) 273, 516
MaeIII GTNAC 2 cut(s) 889, 910
MalI GATC 4 cut(s) 314, 581, 923, 1134
MbiI CCGCTC 2 cut(s) 776, 848
MboI GATC 4 cut(s) 312, 579, 921, 1132
MboII GAAGA 5 cut(s) 46, 52, 598, 1130, 1182
MlsI TGGCCA 3 cut(s) 101, 626, 836
MluI ACGCGT 1 cut(s) 636
MluNI TGGCCA 3 cut(s) 101, 626, 836
Mly113I GGCGCC 5 cut(s) 30, 294, 740, 1022, 1076
MlyI GAGTC 1 cut(s) 1103
MmeI TCCRAC 4 cut(s) 397, 569, 834, 1037
Mox20I TGGCCA 3 cut(s) 101, 626, 836
MreI CGCCGGCG 1 cut(s) 26
MroNI GCCGGC 2 cut(s) 26, 1024
MscI TGGCCA 3 cut(s) 101, 626, 836
MslI CAYNNNNRTG 2 cut(s) 92, 797
Msp20I TGGCCA 3 cut(s) 101, 626, 836
MspR9I CCNGG 8 cut(s) 350, 369, 552, 577, 690, 744, 1085, 1103
MvaI CCWGG 2 cut(s) 350, 552
MvnI CGCG 4 cut(s) 91, 455, 638, 1055
MwoI GCNNNNNNNGC 9 cut(s) 23, 103, 215, 224, 335, 378, 539, 991, 1052
NaeI GCCGGC 2 cut(s) 28, 1026
NarI GGCGCC 5 cut(s) 30, 294, 740, 1022, 1076
NciI CCSGG 6 cut(s) 369, 577, 690, 744, 1085, 1103
NdeII GATC 4 cut(s) 312, 579, 921, 1132
NgoMIV GCCGGC 2 cut(s) 26, 1024
NlaIII CATG 5 cut(s) 97, 214, 346, 586, 796
NmeAIII GCCGAG 4 cut(s) 562, 586, 637, 716
NmuCI GTSAC 2 cut(s) 889, 910
NspI RCATGY 1 cut(s) 97
PaeI GCATGC 1 cut(s) 97
PcsI WCGNNNNNNNCGW 6 cut(s) 279, 468, 657, 660, 864, 1128
PdiI GCCGGC 2 cut(s) 28, 1026
PfeI GAWTC 2 cut(s) 1097, 1192
PleI GAGTC 1 cut(s) 1103
PluTI GGCGCC 5 cut(s) 33, 297, 743, 1025, 1079
PpsI GAGTC 1 cut(s) 1103
Psp6I CCWGG 2 cut(s) 348, 550
PspEI GGTNACC 1 cut(s) 889
PspFI CCCAGC 1 cut(s) 1126
PspGI CCWGG 2 cut(s) 348, 550
PspPI GGNCC 3 cut(s) 37, 554, 589
RsaI GTAC 4 cut(s) 767, 785, 857, 998
RsaNI GTAC 4 cut(s) 766, 784, 856, 997
RseI CAYNNNNRTG 2 cut(s) 92, 797
SalI GTCGAC 3 cut(s) 197, 473, 662
Sau3AI GATC 4 cut(s) 312, 579, 921, 1132
Sau96I GGNCC 3 cut(s) 37, 554, 589
SchI GAGTC 1 cut(s) 1103
ScrFI CCNGG 8 cut(s) 350, 369, 552, 577, 690, 744, 1085, 1103
SfoI GGCGCC 5 cut(s) 31, 295, 741, 1023, 1077
SgrAI CRCCGGYG 1 cut(s) 26
SgrDI CGTCGACG 2 cut(s) 473, 662
SinI GGWCC 1 cut(s) 37
SmiMI CAYNNNNRTG 2 cut(s) 92, 797
SmlI CTYRAG 2 cut(s) 401, 896
SmoI CTYRAG 2 cut(s) 401, 896
SphI GCATGC 1 cut(s) 97
SspDI GGCGCC 5 cut(s) 29, 293, 739, 1021, 1075
SspMI CTAG 1 cut(s) 908
StyD4I CCNGG 8 cut(s) 348, 367, 550, 575, 688, 742, 1083, 1101
StyI CCWWGG 3 cut(s) 33, 987, 1207
TaaI ACNGT 6 cut(s) 187, 679, 868, 881, 1151, 1221
TaiI ACGT 2 cut(s) 276, 519
TaqI TCGA 6 cut(s) 198, 282, 474, 651, 663, 939
TatI WGTACW 1 cut(s) 996
TauI GCSGC 7 cut(s) 158, 338, 455, 533, 536, 776, 1049
TfiI GAWTC 2 cut(s) 1097, 1192
TscAI CASTG 3 cut(s) 1156, 1204, 1224
TseFI GTSAC 2 cut(s) 889, 910
TseI GCWGC 4 cut(s) 4, 597, 752, 946
Tsp45I GTSAC 2 cut(s) 889, 910
TspDTI ATGAA 1 cut(s) 839
TspGWI ACGGA 5 cut(s) 34, 55, 460, 487, 858
TspRI CASTG 3 cut(s) 1156, 1204, 1224
VpaK11BI GGWCC 1 cut(s) 37
XceI RCATGY 1 cut(s) 97
XmiI GTMKAC 3 cut(s) 198, 474, 663
XspI CTAG 1 cut(s) 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.