RchiOBHm_Chr5g0061241
WRKY Family

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015301
Physical Location & Seq
Reverse (-)
66625179 .. 66627443
2265 bp
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UTR
Exon/CDS
Intron
PRQ33763

Sequence Viewer

Length: 678 bp
ATGGCAAAAAGTAATCCTGATGAAAAGATTCTCAGCTACAATGATGTGGTACTTAGACGATCAGACTTGGATATCCTCAGCGGCCCATATTTTCTCAATGATCGAATCATCGAGTTCTATTTTAGTTATCTTTCATCATGCTCTGAGGAGATTTTACTCATTCCACCTTCAATTACATTCTGGATAATGAATTGCCCAGCTGAGAGCCTAAAAGACTTCCTAGACCCTCTTCATTTACCTGATAAGAAACTGGTAATCTTTCCTGTCAATGACAATGATGATGTGAGTGAAGCTGAAGGTGGATATCATTGGAGCCTACTTGCATTTGAGAGAAGCATCAACACATTTGTTCATCATGATAGCAATGGAGGGGGATGGAATAAAGGTCCCGCCAAACGACTTTATAATGCTGTTAAGAAATTTATGAGTGATTCCAGTTCAACAACTGAGCCCATTTATAAAGAGTGTACTGATTCACCACACCAAGTAAATGGTTACGATTGTGGCATGTATGTATTGGCCATTGCAAGGATAATATGCAGCTGGTATGGAAGTAAAGACAAAGATGGAGATAATATGTGGTTCTGTGCTGTGAAGGAACAAGTCACTCCATCGGCTGTCACTGCAATGCGAGGTGAGATCCTGGGGTTGATCAGAGGTTTGATGCCCAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0016787 GO:0019538 GO:0019783 GO:0019784 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C48 WRKY

Protein Analysis

225

Amino Acids

25.42

Weight (kDa)

5.24

Isoelectric Point (pI)

55.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 78 - 179 1.9e-11 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015301)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G60190
fragaria_vesca FvH4_3g33710
malus_domestica MD09G1211300.v1.1 MD11G1123000.v1.1
prunus_persica Prupe.6G092400_v2.0.a1
pyrus_communis pycom11g10400
rosa_chinensis RchiOBHm_Chr5g0061241
rosa_laevigata RLG00000035445
rosa_multiflora Rmu_sc0007612.1_g000045
rosa_roxburghii Rroxscaffold_1G00019460
rosa_rugosa Rorug05G0339100
rosa_samantha Rh5AG400200 Rh5BG413200 Rh5CG437700 Rh5DG427400
rosa_wichuraiana Rw5G037710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 405, 459
AciI CCGC 2 cut(s) 81, 390
AclWI GGATC 1 cut(s) 634
AcoI YGGCCR 1 cut(s) 519
AcsI RAATTY 1 cut(s) 419
AcuI CTGAAG 1 cut(s) 315
AfaI GTAC 2 cut(s) 51, 469
AfiI CCNNNNNNNGG 1 cut(s) 528
AgsI TTSAA 2 cut(s) 171, 441
AjnI CCWGG 1 cut(s) 642
AluBI AGCT 4 cut(s) 36, 200, 293, 543
AluI AGCT 4 cut(s) 36, 200, 293, 543
AlwI GGATC 1 cut(s) 634
AoxI GGCC 2 cut(s) 82, 519
ApeKI GCWGC 1 cut(s) 540
ApoI RAATTY 1 cut(s) 419
Asp700I GAANNNNTTC 1 cut(s) 27
AspS9I GGNCC 2 cut(s) 83, 386
AsuHPI GGTGA 2 cut(s) 468, 647
AvaII GGWCC 1 cut(s) 386
BalI TGGCCA 1 cut(s) 521
BanII GRGCYC 1 cut(s) 453
BbvCI CCTCAGC 1 cut(s) 77
BbvI GCAGC 1 cut(s) 552
BccI CCATC 3 cut(s) 369, 560, 619
BciT130I CCWGG 1 cut(s) 644
BclI TGATCA 1 cut(s) 651
BfaI CTAG 1 cut(s) 221
BisI GCNGC 2 cut(s) 82, 541
BlsI GCNGC 2 cut(s) 83, 542
Bme1390I CCNGG 1 cut(s) 644
Bme18I GGWCC 1 cut(s) 386
BmgT120I GGNCC 2 cut(s) 83, 386
BmiI GGNNCC 2 cut(s) 314, 388
BmrFI CCNGG 1 cut(s) 644
BmsI GCATC 2 cut(s) 345, 654
Bpu10I CCTNAGC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 643
Bsc4I CCNNNNNNNGG 1 cut(s) 528
Bse1I ACTGG 2 cut(s) 255, 435
Bse3DI GCAATG 3 cut(s) 370, 522, 633
BseBI CCWGG 1 cut(s) 644
BseDI CCNNGG 1 cut(s) 643
BseGI GGATG 1 cut(s) 380
BseLI CCNNNNNNNGG 1 cut(s) 528
BseMI GCAATG 3 cut(s) 370, 522, 633
BseMII CTCAG 5 cut(s) 46, 91, 135, 192, 438
BseNI ACTGG 2 cut(s) 255, 435
BseRI GAGGAG 1 cut(s) 161
BseXI GCAGC 1 cut(s) 552
BseYI CCCAGC 1 cut(s) 196
BshFI GGCC 2 cut(s) 84, 521
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 1 cut(s) 528
BsmFI GGGAC 1 cut(s) 372
BsnI GGCC 2 cut(s) 84, 521
Bsp1286I GDGCHC 1 cut(s) 453
Bsp143I GATC 4 cut(s) 59, 100, 639, 651
BspACI CCGC 2 cut(s) 81, 390
BspANI GGCC 2 cut(s) 84, 521
BspCNI CTCAG 5 cut(s) 45, 90, 136, 193, 439
BspHI TCATGA 1 cut(s) 355
BspLI GGNNCC 2 cut(s) 314, 388
BspPI GGATC 1 cut(s) 634
BsrDI GCAATG 3 cut(s) 370, 522, 633
BsrI ACTGG 2 cut(s) 255, 435
BssECI CCNNGG 1 cut(s) 643
BssMI GATC 4 cut(s) 59, 100, 639, 651
Bst2UI CCWGG 1 cut(s) 644
Bst6I CTCTTC 1 cut(s) 234
BstDEI CTNAG 6 cut(s) 32, 53, 77, 144, 201, 447
BstF5I GGATG 1 cut(s) 380
BstKTI GATC 4 cut(s) 62, 103, 642, 654
BstMBI GATC 4 cut(s) 59, 100, 639, 651
BstMWI GCNNNNNNNGC 1 cut(s) 623
BstNI CCWGG 1 cut(s) 644
BstNSI RCATGY 1 cut(s) 511
BstSCI CCNGG 1 cut(s) 642
BstV1I GCAGC 1 cut(s) 552
BstX2I RGATCY 1 cut(s) 639
BstXI CCANNNNNNTGG 1 cut(s) 491
BstYI RGATCY 1 cut(s) 639
BsuRI GGCC 2 cut(s) 84, 521
BtsCI GGATG 1 cut(s) 380
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 1 cut(s) 621
CciI TCATGA 1 cut(s) 355
Cfr13I GGNCC 2 cut(s) 83, 386
Csp6I GTAC 2 cut(s) 50, 468
CviAII CATG 3 cut(s) 138, 356, 508
CviQI GTAC 2 cut(s) 50, 468
DdeI CTNAG 6 cut(s) 32, 53, 77, 144, 201, 447
DpnI GATC 4 cut(s) 61, 102, 641, 653
DpnII GATC 4 cut(s) 59, 100, 639, 651
EaeI YGGCCR 1 cut(s) 519
Eam1104I CTCTTC 1 cut(s) 234
EarI CTCTTC 1 cut(s) 234
Eco24I GRGCYC 1 cut(s) 453
Eco32I GATATC 2 cut(s) 73, 305
Eco47I GGWCC 1 cut(s) 386
Eco57I CTGAAG 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 1 cut(s) 642
EcoRV GATATC 2 cut(s) 73, 305
EcoT38I GRGCYC 1 cut(s) 453
FaeI CATG 3 cut(s) 141, 359, 511
FaqI GGGAC 1 cut(s) 372
FatI CATG 3 cut(s) 137, 355, 507
FauI CCCGC 1 cut(s) 397
FbaI TGATCA 1 cut(s) 651
Fnu4HI GCNGC 2 cut(s) 82, 541
FokI GGATG 1 cut(s) 387
FriOI GRGCYC 1 cut(s) 453
Fsp4HI GCNGC 2 cut(s) 82, 541
FspBI CTAG 1 cut(s) 221
GluI GCNGC 2 cut(s) 82, 541
GsaI CCCAGC 1 cut(s) 200
HaeIII GGCC 2 cut(s) 84, 521
Hin1II CATG 3 cut(s) 141, 359, 511
HinfI GANTC 4 cut(s) 28, 105, 431, 473
HphI GGTGA 2 cut(s) 468, 647
Hpy166II GTNNAC 1 cut(s) 468
Hpy188I TCNGA 3 cut(s) 64, 145, 656
Hpy188III TCNNGA 3 cut(s) 17, 181, 356
Hpy8I GTNNAC 1 cut(s) 468
HpyAV CCTTC 3 cut(s) 177, 290, 589
HpyCH4V TGCA 4 cut(s) 323, 527, 540, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 623
HpyF3I CTNAG 6 cut(s) 32, 53, 77, 144, 201, 447
Hsp92II CATG 3 cut(s) 141, 359, 511
Ksp22I TGATCA 1 cut(s) 651
Kzo9I GATC 4 cut(s) 59, 100, 639, 651
LmnI GCTCC 1 cut(s) 312
Lsp1109I GCAGC 1 cut(s) 552
LweI GCATC 2 cut(s) 345, 654
MaeI CTAG 1 cut(s) 221
MaeIII GTNAC 3 cut(s) 494, 604, 619
MalI GATC 4 cut(s) 61, 102, 641, 653
MboI GATC 4 cut(s) 59, 100, 639, 651
MboII GAAGA 1 cut(s) 221
MflI RGATCY 1 cut(s) 639
MhlI GDGCHC 1 cut(s) 453
MlsI TGGCCA 1 cut(s) 521
MluCI AATT 3 cut(s) 171, 190, 419
MluNI TGGCCA 1 cut(s) 521
MnlI CCTC 6 cut(s) 86, 139, 237, 362, 626, 650
Mox20I TGGCCA 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 27
MscI TGGCCA 1 cut(s) 521
MseI TTAA 1 cut(s) 414
MslI CAYNNNNRTG 1 cut(s) 626
Msp20I TGGCCA 1 cut(s) 521
MspA1I CMGCKG 3 cut(s) 81, 200, 543
MspR9I CCNGG 1 cut(s) 644
MvaI CCWGG 1 cut(s) 644
MwoI GCNNNNNNNGC 1 cut(s) 623
NdeII GATC 4 cut(s) 59, 100, 639, 651
NlaIII CATG 3 cut(s) 141, 359, 511
NlaIV GGNNCC 2 cut(s) 314, 388
NmuCI GTSAC 2 cut(s) 604, 619
NspI RCATGY 1 cut(s) 511
PagI TCATGA 1 cut(s) 355
PdmI GAANNNNTTC 1 cut(s) 27
PfeI GAWTC 4 cut(s) 28, 105, 431, 473
PkrI GCNGC 2 cut(s) 83, 542
PpuMI RGGWCCY 1 cut(s) 386
PsiI TTATAA 2 cut(s) 405, 459
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 1 cut(s) 642
PspFI CCCAGC 1 cut(s) 196
PspGI CCWGG 1 cut(s) 642
PspN4I GGNNCC 2 cut(s) 314, 388
PspPI GGNCC 2 cut(s) 83, 386
PspPPI RGGWCCY 1 cut(s) 386
PsuI RGATCY 1 cut(s) 639
PvuII CAGCTG 2 cut(s) 200, 543
RsaI GTAC 2 cut(s) 51, 469
RsaNI GTAC 2 cut(s) 50, 468
RseI CAYNNNNRTG 1 cut(s) 626
SaqAI TTAA 1 cut(s) 414
SatI GCNGC 2 cut(s) 82, 541
Sau3AI GATC 4 cut(s) 59, 100, 639, 651
Sau96I GGNCC 2 cut(s) 83, 386
ScrFI CCNGG 1 cut(s) 644
SduI GDGCHC 1 cut(s) 453
SfaNI GCATC 2 cut(s) 345, 654
SinI GGWCC 1 cut(s) 386
SmiMI CAYNNNNRTG 1 cut(s) 626
Sse9I AATT 3 cut(s) 171, 190, 419
SsiI CCGC 2 cut(s) 81, 390
SspMI CTAG 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 642
TaqI TCGA 2 cut(s) 103, 111
TasI AATT 3 cut(s) 171, 190, 419
TatI WGTACW 1 cut(s) 467
TauI GCSGC 1 cut(s) 84
TfiI GAWTC 4 cut(s) 28, 105, 431, 473
Tru1I TTAA 1 cut(s) 414
Tru9I TTAA 1 cut(s) 414
TscAI CASTG 1 cut(s) 628
TseFI GTSAC 2 cut(s) 604, 619
TseI GCWGC 1 cut(s) 540
Tsp45I GTSAC 2 cut(s) 604, 619
TspDTI ATGAA 5 cut(s) 36, 123, 203, 221, 341
TspRI CASTG 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 386
XapI RAATTY 1 cut(s) 419
XceI RCATGY 1 cut(s) 511
XmnI GAANNNNTTC 1 cut(s) 27
XspI CTAG 1 cut(s) 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.