Rorug05G0339100
WRKY Family

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009092
Physical Location & Seq
Forward (+)
41201091 .. 41204778
3688 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0339100.1

Sequence Viewer

Length: 1092 bp
ATGGAGTTGGGTGGTGGAGAGAGAAAACGAGTGGTGGTGATCGGAGGCGGCGTTGCTGGCTCGCTTCTCGCAAAAAATCTGCAATTCAGCGCTGATTTCACCCTCATTGATCAGAAGGAATATTTTGAGATTCCATGGGCAAGCTTGAGGACCATGGTGGAGCCATCATTTGGGGAGAGATCTGTGATCAACCATAGAGATTACCTCACAAATGGCCAAATTATTACATCTGGAGCAATCAATATCACTGACACTGAAGTATTGACGGGAGAAGGCCGTCTAATTCCCTATGACTACCTTGTCATTGCCACTGGCCATGCAGATGCTGTTCCTAAAACCAAGACTGGGAGGCTTAAGGAATACCAAGAAGATAATCAGAAGATAAGATCAGCTAATTCCATTTTGATTGTTGGAGGAGGACCTACTGGTGTAGAGCTTGCTGGAGAAATTGTTACTGACTTCCCAGATAAGAAAATCACTCTGGTTCATACTGGTTCAAGGTTGTTGGAATTCATTGGACCAAAAGCTGCAAACAAGACTCTTAATTGGTTGCAGTCAAGGAATGTTGAAGTGATACTGGAGCAATCAGTCAGTTTAAACACAATCTCCGAGTCACCTGGAAGCAAAACATATCAGACTTCAGAAGGACAAACGTTTCATGCAGATTGTCATTTTCTATGCACAGGGAAACATGTGGGTTCAGCTTGGCTCAAAGAAACAGTTCTGAAGAGTAGCTTGGATGAATTTGGGAGATTAATAGTTGATGAGAATTTGAGAGTCAATGGGAGAAAAAACATCTTTGCAGTCGGAGACATTACTGATATTAAAGAAATTAAGCAAGGGTATTTGGCGCAAAAACAGGCTTTGGTGGCTGCAAAGAACATAAAGCTCTTGCTTGCAGGAGGAAGAGAGAGTAAAATGGCAACTTACGAAGCTCATTCGGTGCTAGCACTTGTTTCACTTGGAAGGAAACATGGGCTTGCACAGTTCCCATACACTACTTGTATTGGACGCCTTCCTGGACTGATCAAATCTAAAGATCTTTTTGTGGGGAAGACAAGGAATCAGCTGGGGCTACTGCCACAAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

363

Amino Acids

39.62

Weight (kDa)

8.91

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 10 - 288 1.5e-36 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox_3 PF13738 98 - 270 1.2e-06 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 134 - 197 2.1e-07 Pyridine nucleotide-disulphide oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 299
AccB7I CCANNNNNTGG 1 cut(s) 170
AciI CCGC 1 cut(s) 48
AclI AACGTT 1 cut(s) 653
AcoI YGGCCR 2 cut(s) 214, 313
AcsI RAATTY 3 cut(s) 509, 743, 769
AcuI CTGAAG 3 cut(s) 276, 624, 746
AcyI GRCGYC 1 cut(s) 1012
AfeI AGCGCT 1 cut(s) 91
AfiI CCNNNNNNNGG 2 cut(s) 170, 345
AflII CTTAAG 1 cut(s) 353
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 2 cut(s) 498, 569
AjnI CCWGG 2 cut(s) 616, 1018
AjuI GAANNNNNNNTTGG 2 cut(s) 719, 751
AluBI AGCT 9 cut(s) 144, 392, 436, 527, 704, 735, 889, 935, 1069
AluI AGCT 9 cut(s) 144, 392, 436, 527, 704, 735, 889, 935, 1069
Alw26I GTCTC 1 cut(s) 804
AlwNI CAGNNNCTG 1 cut(s) 326
Aor51HI AGCGCT 1 cut(s) 91
AoxI GGCC 3 cut(s) 214, 274, 313
ApeKI GCWGC 2 cut(s) 527, 872
ApoI RAATTY 3 cut(s) 509, 743, 769
AseI ATTAAT 1 cut(s) 755
Asp700I GAANNNNTTC 1 cut(s) 720
AspLEI GCGC 2 cut(s) 92, 853
AspS9I GGNCC 3 cut(s) 150, 419, 518
AsuHPI GGTGA 3 cut(s) 49, 91, 606
AsuNHI GCTAGC 1 cut(s) 946
AvaII GGWCC 3 cut(s) 150, 419, 518
BalI TGGCCA 2 cut(s) 216, 315
BbsI GAAGAC 1 cut(s) 1061
BbvI GCAGC 2 cut(s) 514, 859
BccI CCATC 1 cut(s) 172
BceAI ACGGC 1 cut(s) 261
BciT130I CCWGG 2 cut(s) 618, 1020
BclI TGATCA 3 cut(s) 109, 186, 1026
BcoDI GTCTC 1 cut(s) 804
BfaI CTAG 1 cut(s) 947
BfoI RGCGCY 1 cut(s) 93
BfrI CTTAAG 1 cut(s) 353
BglII AGATCT 2 cut(s) 179, 1039
BisI GCNGC 3 cut(s) 49, 528, 873
BlsI GCNGC 3 cut(s) 50, 529, 874
Bme1390I CCNGG 2 cut(s) 618, 1020
Bme18I GGWCC 3 cut(s) 150, 419, 518
BmgT120I GGNCC 3 cut(s) 150, 419, 518
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 2 cut(s) 618, 1020
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 1 cut(s) 313
BmtI GCTAGC 1 cut(s) 950
BmuI ACTGGG 1 cut(s) 354
BpiI GAAGAC 1 cut(s) 1061
BplI GAGNNNNNCTC 2 cut(s) 189, 221
BpmI CTGGAG 3 cut(s) 252, 462, 599
BpuEI CTTGAG 1 cut(s) 166
BsaHI GRCGYC 1 cut(s) 1012
BsaJI CCNNGG 2 cut(s) 134, 153
BsaXI ACNNNNNCTCC 6 cut(s) 36, 66, 435, 465, 590, 620
Bsc4I CCNNNNNNNGG 2 cut(s) 170, 345
Bse1I ACTGG 5 cut(s) 316, 349, 430, 496, 582
Bse3DI GCAATG 1 cut(s) 303
BseBI CCWGG 2 cut(s) 618, 1020
BseDI CCNNGG 2 cut(s) 134, 153
BseGI GGATG 1 cut(s) 745
BseLI CCNNNNNNNGG 2 cut(s) 170, 345
BseMI GCAATG 1 cut(s) 303
BseNI ACTGG 5 cut(s) 316, 349, 430, 496, 582
BseRI GAGGAG 1 cut(s) 429
BseXI GCAGC 2 cut(s) 514, 859
BseYI CCCAGC 1 cut(s) 1069
BshFI GGCC 3 cut(s) 216, 276, 315
BslI CCNNNNNNNGG 2 cut(s) 170, 345
BsmAI GTCTC 1 cut(s) 804
BsnI GGCC 3 cut(s) 216, 276, 315
Bsp143I GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
Bsp19I CCATGG 2 cut(s) 134, 153
BspACI CCGC 1 cut(s) 48
BspANI GGCC 3 cut(s) 216, 276, 315
BspLI GGNNCC 1 cut(s) 162
BspOI GCTAGC 1 cut(s) 950
BspTI CTTAAG 1 cut(s) 353
BsrDI GCAATG 1 cut(s) 303
BsrI ACTGG 5 cut(s) 316, 349, 430, 496, 582
BssECI CCNNGG 2 cut(s) 134, 153
BssMI GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
BssNI GRCGYC 1 cut(s) 1012
BssT1I CCWWGG 2 cut(s) 134, 153
Bst2UI CCWGG 2 cut(s) 618, 1020
Bst4CI ACNGT 2 cut(s) 721, 987
Bst6I CTCTTC 2 cut(s) 722, 901
BstACI GRCGYC 1 cut(s) 1012
BstAFI CTTAAG 1 cut(s) 353
BstC8I GCNNGC 7 cut(s) 58, 62, 142, 438, 897, 948, 981
BstDSI CCRYGG 2 cut(s) 134, 153
BstF5I GGATG 1 cut(s) 745
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 2 cut(s) 92, 853
BstKTI GATC 7 cut(s) 42, 112, 182, 189, 389, 1029, 1042
BstMAI GTCTC 1 cut(s) 804
BstMBI GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
BstMWI GCNNNNNNNGC 2 cut(s) 57, 869
BstNI CCWGG 2 cut(s) 618, 1020
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 2 cut(s) 616, 1018
BstV1I GCAGC 2 cut(s) 514, 859
BstV2I GAAGAC 1 cut(s) 1061
BstX2I RGATCY 2 cut(s) 179, 1039
BstYI RGATCY 2 cut(s) 179, 1039
BsuRI GGCC 3 cut(s) 216, 276, 315
BtgI CCRYGG 2 cut(s) 134, 153
BtsCI GGATG 1 cut(s) 745
BtsIMutI CAGTG 3 cut(s) 246, 252, 309
Cac8I GCNNGC 7 cut(s) 58, 62, 142, 438, 897, 948, 981
CaiI CAGNNNCTG 1 cut(s) 326
CfoI GCGC 2 cut(s) 92, 853
Cfr13I GGNCC 3 cut(s) 150, 419, 518
CseI GACGC 1 cut(s) 1020
CviAII CATG 6 cut(s) 135, 154, 317, 659, 692, 974
DpnI GATC 7 cut(s) 41, 111, 181, 188, 388, 1028, 1041
DpnII GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
DraI TTTAAA 1 cut(s) 597
DrdI GACNNNNNNGTC 1 cut(s) 299
DseDI GACNNNNNNGTC 1 cut(s) 299
EaeI YGGCCR 2 cut(s) 214, 313
Eam1104I CTCTTC 2 cut(s) 722, 901
EarI CTCTTC 2 cut(s) 722, 901
Eco130I CCWWGG 2 cut(s) 134, 153
Eco47I GGWCC 3 cut(s) 150, 419, 518
Eco47III AGCGCT 1 cut(s) 91
Eco57I CTGAAG 3 cut(s) 276, 624, 746
EcoO109I RGGNCCY 1 cut(s) 419
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 2 cut(s) 616, 1018
EcoT14I CCWWGG 2 cut(s) 134, 153
ErhI CCWWGG 2 cut(s) 134, 153
FaeI CATG 6 cut(s) 138, 157, 320, 662, 695, 977
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FatI CATG 6 cut(s) 134, 153, 316, 658, 691, 973
FbaI TGATCA 3 cut(s) 109, 186, 1026
Fnu4HI GCNGC 3 cut(s) 49, 528, 873
FokI GGATG 1 cut(s) 752
Fsp4HI GCNGC 3 cut(s) 49, 528, 873
FspBI CTAG 1 cut(s) 947
GlaI GCGC 2 cut(s) 91, 852
GluI GCNGC 3 cut(s) 49, 528, 873
GsaI CCCAGC 1 cut(s) 1073
GsuI CTGGAG 3 cut(s) 252, 462, 599
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 3 cut(s) 216, 276, 315
HgaI GACGC 1 cut(s) 1020
HhaI GCGC 2 cut(s) 92, 853
Hin1I GRCGYC 1 cut(s) 1012
Hin1II CATG 6 cut(s) 138, 157, 320, 662, 695, 977
Hin6I GCGC 2 cut(s) 90, 851
HinP1I GCGC 2 cut(s) 90, 851
HindIII AAGCTT 1 cut(s) 142
HinfI GANTC 5 cut(s) 130, 538, 611, 777, 1063
HphI GGTGA 3 cut(s) 49, 91, 606
Hpy188I TCNGA 8 cut(s) 44, 114, 378, 610, 636, 643, 726, 809
Hpy188III TCNNGA 1 cut(s) 231
HpyAV CCTTC 5 cut(s) 109, 266, 638, 960, 1025
HpyCH4III ACNGT 2 cut(s) 721, 987
HpyCH4IV ACGT 1 cut(s) 653
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 869
HpySE526I ACGT 1 cut(s) 653
Hsp92I GRCGYC 1 cut(s) 1012
Hsp92II CATG 6 cut(s) 138, 157, 320, 662, 695, 977
HspAI GCGC 2 cut(s) 90, 851
Ksp22I TGATCA 3 cut(s) 109, 186, 1026
Kzo9I GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
LmnI GCTCC 3 cut(s) 160, 233, 580
Lsp1109I GCAGC 2 cut(s) 514, 859
LweI GCATC 1 cut(s) 313
MaeI CTAG 1 cut(s) 947
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 2 cut(s) 451, 612
MalI GATC 7 cut(s) 41, 111, 181, 188, 388, 1028, 1041
MboI GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
MboII GAAGA 5 cut(s) 380, 391, 739, 918, 1066
MflI RGATCY 2 cut(s) 179, 1039
MlsI TGGCCA 2 cut(s) 216, 315
MluNI TGGCCA 2 cut(s) 216, 315
MlyI GAGTC 3 cut(s) 532, 620, 786
MmeI TCCRAC 3 cut(s) 391, 486, 787
MnlI CCTC 8 cut(s) 38, 113, 141, 215, 342, 407, 410, 896
Mox20I TGGCCA 2 cut(s) 216, 315
MroXI GAANNNNTTC 1 cut(s) 720
MscI TGGCCA 2 cut(s) 216, 315
MseI TTAA 7 cut(s) 354, 543, 596, 755, 825, 834, 1090
MslI CAYNNNNRTG 1 cut(s) 321
Msp20I TGGCCA 2 cut(s) 216, 315
MspA1I CMGCKG 1 cut(s) 1069
MspCI CTTAAG 1 cut(s) 353
MspR9I CCNGG 2 cut(s) 618, 1020
MssI GTTTAAAC 1 cut(s) 597
MvaI CCWGG 2 cut(s) 618, 1020
MwoI GCNNNNNNNGC 2 cut(s) 57, 869
NcoI CCATGG 2 cut(s) 134, 153
NdeII GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
NheI GCTAGC 1 cut(s) 946
NlaIII CATG 6 cut(s) 138, 157, 320, 662, 695, 977
NlaIV GGNNCC 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 612
NspI RCATGY 1 cut(s) 695
PciI ACATGT 1 cut(s) 691
PcsI WCGNNNNNNNCGW 1 cut(s) 48
PdmI GAANNNNTTC 1 cut(s) 720
PfeI GAWTC 2 cut(s) 130, 1063
PflMI CCANNNNNTGG 1 cut(s) 170
PfoI TCCNGGA 1 cut(s) 1018
PkrI GCNGC 3 cut(s) 50, 529, 874
PleI GAGTC 3 cut(s) 532, 619, 785
PmeI GTTTAAAC 1 cut(s) 597
PpsI GAGTC 3 cut(s) 532, 619, 785
PpuMI RGGWCCY 1 cut(s) 419
PscI ACATGT 1 cut(s) 691
PshBI ATTAAT 1 cut(s) 755
Psp1406I AACGTT 1 cut(s) 653
Psp5II RGGWCCY 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 616, 1018
PspFI CCCAGC 1 cut(s) 1069
PspGI CCWGG 2 cut(s) 616, 1018
PspN4I GGNNCC 1 cut(s) 162
PspPI GGNCC 3 cut(s) 150, 419, 518
PspPPI RGGWCCY 1 cut(s) 419
PstNI CAGNNNCTG 1 cut(s) 326
PsuI RGATCY 2 cut(s) 179, 1039
PvuII CAGCTG 1 cut(s) 1069
RseI CAYNNNNRTG 1 cut(s) 321
SaqAI TTAA 7 cut(s) 354, 543, 596, 755, 825, 834, 1090
SatI GCNGC 3 cut(s) 49, 528, 873
Sau3AI GATC 7 cut(s) 39, 109, 179, 186, 386, 1026, 1039
Sau96I GGNCC 3 cut(s) 150, 419, 518
SchI GAGTC 3 cut(s) 532, 620, 786
ScrFI CCNGG 2 cut(s) 618, 1020
SfaNI GCATC 1 cut(s) 313
SinI GGWCC 3 cut(s) 150, 419, 518
SmiMI CAYNNNNRTG 1 cut(s) 321
SmlI CTYRAG 2 cut(s) 145, 353
SmoI CTYRAG 2 cut(s) 145, 353
SsiI CCGC 1 cut(s) 48
SspI AATATT 1 cut(s) 122
SspMI CTAG 1 cut(s) 947
StyD4I CCNGG 2 cut(s) 616, 1018
StyI CCWWGG 2 cut(s) 134, 153
TaaI ACNGT 2 cut(s) 721, 987
TaiI ACGT 1 cut(s) 656
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 2 cut(s) 130, 1063
Tru1I TTAA 7 cut(s) 354, 543, 596, 755, 825, 834, 1090
Tru9I TTAA 7 cut(s) 354, 543, 596, 755, 825, 834, 1090
TscAI CASTG 3 cut(s) 253, 259, 316
TseFI GTSAC 1 cut(s) 612
TseI GCWGC 2 cut(s) 527, 872
Tsp45I GTSAC 1 cut(s) 612
TspDTI ATGAA 4 cut(s) 476, 502, 647, 756
TspRI CASTG 3 cut(s) 253, 259, 316
Van91I CCANNNNNTGG 1 cut(s) 170
Vha464I CTTAAG 1 cut(s) 353
VpaK11BI GGWCC 3 cut(s) 150, 419, 518
VspI ATTAAT 1 cut(s) 755
XapI RAATTY 3 cut(s) 509, 743, 769
XceI RCATGY 1 cut(s) 695
XmnI GAANNNNTTC 1 cut(s) 720
XspI CTAG 1 cut(s) 947
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.