MD11G1122900.v1.1

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009092
Physical Location & Seq
Reverse (-)
11305985 .. 11314800
8816 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1122900.v1.1.491

Sequence Viewer

Length: 2076 bp
ATGGAGAAGTTCATCGACAACTTACCAGCCATGGATCTGATGCGCTCCGAGAAGATGACCTTCGTCCAGCTCATCATCCCCGTCGAGTCCGCCCACCGCGCCATTTCTTACCTCGGCGAACTCGGCCTCCTCCAATTCCGAGACTTAAATGCTGATAAAAGTCCTTTCCAACGAACATTTGTTAATCAGGTAAAGCGATGTGCAGAAATGTCAAGAAAGCTGCGGTTTTTCAGAGATCAAATTAGTAAAGCTGGTCTACTTTCATCTGTACATCCTGTTTTACAACCAGATATTGACTTAGAGGAATTAGAGATACAATTAGCCGAGCATGAGCATGAGCTGATTGAGATGAATTCTAATAGTGATAGACTTCAACATTCATACAATGAACTACTCGAGTTCAAGATGGTATTGCAAAAGGCAAGTGGCTTTCTTGTTTCAAGTAACAGCCATGCGGTTTCAGAGGAGAGAGAACTAGATGAGAATATTTACTCAAATGATAACTATGGTGATGAAGTTTCATTACTTGAGCAGGATATCAGACCTGGCCCATCAGACCAATCTGGTTTGAGATTTGTTAGTGGAATTATTTGTAAATCCAAAGCTCTCAGGTTTGAGAGGATGTTGTTTCGTGCTACAAGGGGCAACATGCTTTTCAACCATGCCCCAGCAGATGAACAGATCATGGATCCTTTATCTACTGAAATGGTTGAGAAAACAGTGTTTGTAGTGTTCTTCTCCGGGATGCAGGCAAAAACAAAGATTTTGAAAATTTGTGAGGCCTTTGGTGCGAATTGCTATCCTGTTCCTGAAGATATAACTAAGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAACAACCTTGGATGCAGGGATTCGTCACAGAAATAAGGCCCTTACATCTGTAGGGTTTCACCTAGCAAAATGGATCAACATGGTAAGAAGGGAGAAAGCTGTATATGATACCTTGAATATGCTAAACTTTGATGTTACAAAAAAATGTCTCGTTGGAGAGGGCTGGTGTCCTATATTTGCAAAACCTAAGATTCAGGAGGCACTGCAACGTGCTACATTTGATAGCAGTTCGCAAGTGGGAGTAATATTTCATGTGATGGATACAGTAGATTCACCTCCTACATATTTCAGAACCAACCGCTTTACAAGTGCATTTCAGGAAATTGTTGATGCATACGGTGTTGCAAGATATCAAGAAGCCAATCCTGCAGTTTACACTTGTATTACATTTCCATTTCTTTTTGCGGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTATTGCTCGGAGCATTAGTTCTTATAGCTCGTGAAAGTAAACTCAGTGCTCAGAAACTTGGGAGCTTTATGGAGATGCTATTTGGTGGGCGATATGTACTACTCTTGATGTCCCTATTTTCAATCTACTGTGGGTTGATTTACAATGAGTTCTTTTCTGTTCCTTTTCACATATTTGGTGGGTCTGCTTACAAATGTAGAGATGCTGCTTGCAGTGAGGCGCACACTATTGGCTTAATCAAGTACAGAGATCCATACCCATTTGGTGTGGATCCCAGTTGGCGTGGAAGTCGTTCTGAACTCCCTTTTCTGAATTCTCTCAAAATGAAAATGTCAATTTTGTTGGGTGTTGTGCAAATGAACTTAGGAATTCTACTAAGTTACTTCAATGCAAGGTTTTTCAGCAGCTCGCTTGATATAAGGTACCAGTTTGTGCCACAGATGATCTTTCTTAACAGTCTTTTTGGATATCTTTCACTTCTTGTTGTCATCAAGTGGTGTACAGGATCTCAGGCAGACCTCTATCATGTAATGATTTACATGTTTTTAAGTCCGACTGATGATCTTGGTGAAAATCAATTATTCTGGGGCCAAAGACCACTTCAGGCATGCATTCTGCTCCTCTTTTCCTTTACTTCGTATATGCAACTTCTTATGTGTTCCATGGATGCTCTTTCCAAAACCTTTCATATTGAGGAAGCTTCATACAGAGAGGTTTCAAGGTCGTGCGTATGGGATGCTTGGCACCTCAGAGATGGATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

692

Amino Acids

78.92

Weight (kDa)

6.1

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 37 - 655 3e-218 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1734
AccB1I GGYRCC 2 cut(s) 1734, 2055
AccI GTMKAC 1 cut(s) 256
AccII CGCG 1 cut(s) 99
AciI CCGC 6 cut(s) 90, 97, 223, 455, 1171, 1277
AclWI GGATC 8 cut(s) 42, 683, 696, 953, 1556, 1577, 1590, 1825
AcsI RAATTY 4 cut(s) 352, 771, 1624, 1680
AcuI CTGAAG 2 cut(s) 831, 1898
AfaI GTAC 5 cut(s) 270, 1410, 1556, 1736, 1813
AflIII ACRYGT 1 cut(s) 1851
AgsI TTSAA 9 cut(s) 374, 403, 441, 658, 769, 988, 1434, 1699, 2031
AjiI CACGTC 1 cut(s) 857
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 1896, 1928
AloI GAACNNNNNNTCC 4 cut(s) 111, 143, 1314, 1346
Alw21I GWGCWC 1 cut(s) 1363
Alw26I GTCTC 2 cut(s) 135, 1025
AlwI GGATC 8 cut(s) 42, 683, 696, 953, 1556, 1577, 1590, 1825
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 5 cut(s) 124, 547, 780, 909, 1900
ApeKI GCWGC 3 cut(s) 220, 1517, 1716
ApoI RAATTY 4 cut(s) 352, 771, 1624, 1680
Asp700I GAANNNNTTC 1 cut(s) 1603
Asp718I GGTACC 1 cut(s) 1734
AspLEI GCGC 3 cut(s) 45, 101, 1534
AspS9I GGNCC 3 cut(s) 548, 910, 1900
AsuC2I CCSGG 1 cut(s) 742
AsuHPI GGTGA 5 cut(s) 521, 923, 1137, 1291, 1892
AvaI CYCGRG 1 cut(s) 395
BamHI GGATCC 2 cut(s) 688, 1582
BanI GGYRCC 2 cut(s) 1734, 2055
BarI GAAGNNNNNNTAC 2 cut(s) 507, 539
BauI CACGAG 1 cut(s) 1341
Bbv12I GWGCWC 1 cut(s) 1363
BbvI GCAGC 3 cut(s) 207, 1504, 1728
BccI CCATC 4 cut(s) 400, 559, 1123, 2060
BciT130I CCWGG 1 cut(s) 546
BciVI GTATCC 1 cut(s) 1126
BcnI CCSGG 1 cut(s) 742
BcoDI GTCTC 2 cut(s) 135, 1025
BfaI CTAG 3 cut(s) 476, 840, 935
BfmI CTRYAG 2 cut(s) 921, 1239
BfuI GTATCC 1 cut(s) 1126
BisI GCNGC 3 cut(s) 221, 1518, 1717
BlsI GCNGC 3 cut(s) 222, 1519, 1718
Bme1390I CCNGG 2 cut(s) 546, 742
BmeT110I CYCGRG 1 cut(s) 395
BmgBI CACGTC 1 cut(s) 857
BmgT120I GGNCC 3 cut(s) 548, 910, 1900
BmiI GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2057
BmrFI CCNGG 2 cut(s) 546, 742
BmrI ACTGGG 2 cut(s) 1303, 1581
BmsI GCATC 8 cut(s) 30, 735, 874, 1192, 1377, 1504, 1969, 2038
BmuI ACTGGG 2 cut(s) 1303, 1581
BoxI GACNNNNGTC 2 cut(s) 62, 1296
BpuEI CTTGAG 1 cut(s) 548
BpuMI CCSGG 1 cut(s) 742
BsaJI CCNNGG 4 cut(s) 30, 112, 879, 1974
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 1314, 1344
Bse1I ACTGG 3 cut(s) 1298, 1587, 1738
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 4 cut(s) 30, 112, 879, 1974
BseGI GGATG 7 cut(s) 75, 271, 627, 750, 889, 1984, 2053
BseMII CTCAG 5 cut(s) 622, 1369, 1376, 1835, 2074
BseNI ACTGG 3 cut(s) 1298, 1587, 1738
BseRI GAGGAG 3 cut(s) 119, 479, 1922
BseXI GCAGC 3 cut(s) 207, 1504, 1728
BseYI CCCAGC 1 cut(s) 667
BsgI GTGCAG 1 cut(s) 222
Bsh1236I CGCG 1 cut(s) 99
BshFI GGCC 5 cut(s) 126, 549, 782, 911, 1902
BshNI GGYRCC 2 cut(s) 1734, 2055
BsiHKAI GWGCWC 1 cut(s) 1363
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 741
BslFI GGGAC 2 cut(s) 1304, 1408
BsmAI GTCTC 2 cut(s) 135, 1025
BsmFI GGGAC 2 cut(s) 1304, 1408
BsmI GAATGC 1 cut(s) 1923
BsnI GGCC 5 cut(s) 126, 549, 782, 911, 1902
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 1 cut(s) 1363
Bsp1407I TGTACA 2 cut(s) 268, 1811
Bsp19I CCATGG 2 cut(s) 30, 1974
BspACI CCGC 6 cut(s) 90, 97, 223, 455, 1171, 1277
BspANI GGCC 5 cut(s) 126, 549, 782, 911, 1902
BspCNI CTCAG 5 cut(s) 621, 1368, 1375, 1834, 2073
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2057
BspMAI CTGCAG 1 cut(s) 1243
BspPI GGATC 8 cut(s) 42, 683, 696, 953, 1556, 1577, 1590, 1825
BspT107I GGYRCC 2 cut(s) 1734, 2055
BsrGI TGTACA 2 cut(s) 268, 1811
BsrI ACTGG 3 cut(s) 1298, 1587, 1738
BssECI CCNNGG 4 cut(s) 30, 112, 879, 1974
BssSI CACGAG 1 cut(s) 1341
BssT1I CCWWGG 3 cut(s) 30, 879, 1974
Bst2BI CACGAG 1 cut(s) 1341
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 5 cut(s) 721, 1138, 1211, 1442, 1769
BstAUI TGTACA 2 cut(s) 268, 1811
BstC8I GCNNGC 4 cut(s) 750, 1522, 1721, 1921
BstDSI CCRYGG 2 cut(s) 30, 1974
BstF5I GGATG 7 cut(s) 75, 271, 627, 750, 889, 1984, 2053
BstFNI CGCG 1 cut(s) 99
BstHHI GCGC 3 cut(s) 45, 101, 1534
BstMAI GTCTC 2 cut(s) 135, 1025
BstMWI GCNNNNNNNGC 4 cut(s) 98, 123, 788, 1238
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 3 cut(s) 652, 1855, 1923
BstPAI GACNNNNGTC 2 cut(s) 62, 1296
BstSCI CCNGG 2 cut(s) 544, 740
BstSFI CTRYAG 2 cut(s) 921, 1239
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 207, 1504, 1728
BstX2I RGATCY 6 cut(s) 34, 688, 1561, 1582, 1817, 2069
BstYI RGATCY 6 cut(s) 34, 688, 1561, 1582, 1817, 2069
BsuI GTATCC 1 cut(s) 1126
BsuRI GGCC 5 cut(s) 126, 549, 782, 911, 1902
BtgI CCRYGG 2 cut(s) 30, 1974
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 857
BtsCI GGATG 7 cut(s) 75, 271, 627, 750, 889, 1984, 2053
BtsI GCAGTG 2 cut(s) 1073, 1531
BtsIMutI CAGTG 4 cut(s) 726, 1073, 1363, 1531
Cac8I GCNNGC 4 cut(s) 750, 1522, 1721, 1921
CfoI GCGC 3 cut(s) 45, 101, 1534
Cfr13I GGNCC 3 cut(s) 548, 910, 1900
Csp6I GTAC 5 cut(s) 269, 1409, 1555, 1735, 1812
CviQI GTAC 5 cut(s) 269, 1409, 1555, 1735, 1812
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 3 cut(s) 30, 879, 1974
Eco147I AGGCCT 1 cut(s) 782
Eco32I GATATC 3 cut(s) 538, 1223, 1781
Eco57I CTGAAG 2 cut(s) 831, 1898
Eco88I CYCGRG 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 3 cut(s) 352, 1624, 1680
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 3 cut(s) 538, 1223, 1781
EcoT14I CCWWGG 3 cut(s) 30, 879, 1974
EcoT22I ATGCAT 2 cut(s) 1207, 1925
ErhI CCWWGG 3 cut(s) 30, 879, 1974
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 2 cut(s) 1304, 1408
FblI GTMKAC 1 cut(s) 256
Fnu4HI GCNGC 3 cut(s) 221, 1518, 1717
FokI GGATG 7 cut(s) 62, 258, 634, 757, 896, 1991, 2060
Fsp4HI GCNGC 3 cut(s) 221, 1518, 1717
FspBI CTAG 3 cut(s) 476, 840, 935
GlaI GCGC 3 cut(s) 44, 100, 1533
GluI GCNGC 3 cut(s) 221, 1518, 1717
GsaI CCCAGC 1 cut(s) 671
HaeIII GGCC 5 cut(s) 126, 549, 782, 911, 1902
HapII CCGG 1 cut(s) 741
HhaI GCGC 3 cut(s) 45, 101, 1534
Hin6I GCGC 3 cut(s) 43, 99, 1532
HinP1I GCGC 3 cut(s) 43, 99, 1532
HindIII AAGCTT 1 cut(s) 2010
HinfI GANTC 4 cut(s) 86, 892, 1063, 1142
HpaII CCGG 1 cut(s) 741
HphI GGTGA 5 cut(s) 521, 923, 1137, 1291, 1892
Hpy166II GTNNAC 4 cut(s) 257, 1246, 1352, 1812
Hpy188III TCNNGA 9 cut(s) 213, 403, 809, 1067, 1190, 1226, 1343, 1417, 2073
Hpy8I GTNNAC 4 cut(s) 257, 1246, 1352, 1812
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 2 cut(s) 70, 954
HpyCH4III ACNGT 5 cut(s) 721, 1138, 1211, 1442, 1769
HpyCH4IV ACGT 2 cut(s) 856, 1081
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 123, 788, 1238
HpySE526I ACGT 2 cut(s) 856, 1081
HspAI GCGC 3 cut(s) 43, 99, 1532
KpnI GGTACC 1 cut(s) 1738
LmnI GCTCC 4 cut(s) 50, 1322, 1374, 1935
Lsp1109I GCAGC 3 cut(s) 207, 1504, 1728
LweI GCATC 8 cut(s) 30, 735, 874, 1192, 1377, 1504, 1969, 2038
MaeI CTAG 3 cut(s) 476, 840, 935
MaeII ACGT 2 cut(s) 856, 1081
MaeIII GTNAC 4 cut(s) 443, 896, 1006, 1691
MboII GAAGA 3 cut(s) 64, 727, 824
MflI RGATCY 6 cut(s) 34, 688, 1561, 1582, 1817, 2069
MhlI GDGCHC 1 cut(s) 1363
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 193, 1006, 1889
Mph1103I ATGCAT 2 cut(s) 1207, 1925
MroXI GAANNNNTTC 1 cut(s) 1603
MseI TTAA 5 cut(s) 146, 183, 1547, 1764, 1859
MslI CAYNNNNRTG 1 cut(s) 333
MspI CCGG 1 cut(s) 741
MspR9I CCNGG 2 cut(s) 546, 742
Mva1269I GAATGC 1 cut(s) 1923
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 4 cut(s) 98, 123, 788, 1238
NciI CCSGG 1 cut(s) 742
NcoI CCATGG 2 cut(s) 30, 1974
NlaIV GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2057
NmeAIII GCCGAG 3 cut(s) 93, 102, 349
NmuCI GTSAC 1 cut(s) 896
NsiI ATGCAT 2 cut(s) 1207, 1925
NspI RCATGY 3 cut(s) 652, 1855, 1923
PaeI GCATGC 1 cut(s) 1923
PaeR7I CTCGAG 1 cut(s) 395
PceI AGGCCT 1 cut(s) 782
PciI ACATGT 1 cut(s) 1851
PctI GAATGC 1 cut(s) 1923
PdmI GAANNNNTTC 1 cut(s) 1603
PfeI GAWTC 3 cut(s) 892, 1063, 1142
PfoI TCCNGGA 1 cut(s) 740
PkrI GCNGC 3 cut(s) 222, 1519, 1718
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PscI ACATGT 1 cut(s) 1851
PshAI GACNNNNGTC 2 cut(s) 62, 1296
Psp6I CCWGG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2057
PspPI GGNCC 3 cut(s) 548, 910, 1900
PspXI VCTCGAGB 1 cut(s) 395
PstI CTGCAG 1 cut(s) 1243
PsuI RGATCY 6 cut(s) 34, 688, 1561, 1582, 1817, 2069
RsaI GTAC 5 cut(s) 270, 1410, 1556, 1736, 1813
RsaNI GTAC 5 cut(s) 269, 1409, 1555, 1735, 1812
RseI CAYNNNNRTG 1 cut(s) 333
SaqAI TTAA 5 cut(s) 146, 183, 1547, 1764, 1859
SatI GCNGC 3 cut(s) 221, 1518, 1717
Sau96I GGNCC 3 cut(s) 548, 910, 1900
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 546, 742
SduI GDGCHC 1 cut(s) 1363
SfaNI GCATC 8 cut(s) 30, 735, 874, 1192, 1377, 1504, 1969, 2038
SfcI CTRYAG 2 cut(s) 921, 1239
Sfr274I CTCGAG 1 cut(s) 395
SlaI CTCGAG 1 cut(s) 395
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 2 cut(s) 395, 527
SmoI CTYRAG 2 cut(s) 395, 527
SphI GCATGC 1 cut(s) 1923
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 6 cut(s) 90, 97, 223, 455, 1171, 1277
SspI AATATT 2 cut(s) 487, 1119
SspMI CTAG 3 cut(s) 476, 840, 935
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 2 cut(s) 544, 740
StyI CCWWGG 3 cut(s) 30, 879, 1974
TaaI ACNGT 5 cut(s) 721, 1138, 1211, 1442, 1769
TaiI ACGT 2 cut(s) 859, 1084
TaqI TCGA 3 cut(s) 15, 84, 396
TatI WGTACW 4 cut(s) 268, 1408, 1554, 1811
TfiI GAWTC 3 cut(s) 892, 1063, 1142
Tru1I TTAA 5 cut(s) 146, 183, 1547, 1764, 1859
Tru9I TTAA 5 cut(s) 146, 183, 1547, 1764, 1859
TscAI CASTG 4 cut(s) 726, 1080, 1363, 1531
TseFI GTSAC 1 cut(s) 896
TseI GCWGC 3 cut(s) 220, 1517, 1716
Tsp45I GTSAC 1 cut(s) 896
TspRI CASTG 4 cut(s) 726, 1080, 1363, 1531
XapI RAATTY 4 cut(s) 352, 771, 1624, 1680
XceI RCATGY 3 cut(s) 652, 1855, 1923
XhoI CTCGAG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 256
XmnI GAANNNNTTC 1 cut(s) 1603
XspI CTAG 3 cut(s) 476, 840, 935
Zsp2I ATGCAT 2 cut(s) 1207, 1925
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.