Rh5BG413400

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0009092
Physical Location & Seq
Forward (+)
66983882 .. 66993805
9924 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG413400.1

Sequence Viewer

Length: 2256 bp
ATGTCAAGAAAGCTGCGCTTTTTTAAGGATCAAATCAGTAAAGCTGGTCTGCTTGGGTCTATACGTCCAGTTTCACAACCAGATATTGAGTTGGAGGAATTAGAGTTTCAACTTGGTGAGCACGAACATGAGCTAATTGAAATGAACTCTAATAGTGAAAGACTTCGACAATCATACAATGAGCTCCTGGAGTTCAAGATGGTATTACAAAAGGCAAGTGGCTTTCTTGTTTCAAGTAATAGCCATGCTGTCACAGAGGAAACAGAACTAGAAGAGAATGTTTACTCAATGAATGACTATGGAGATTCAGTGTCATTGCTGGAGCAGGATATCAGACGGGGACCATCAGATCAATCTGGTTTGAATTTTGTTAGTGGGATTATTTGTAAATCTAAAGCTCTAAGGTTTGAGAGGATGCTGTTTCGTGCTACAAGGGGAAATATGCTTTTCAACCAAGCACCAGCAGATGAACATATCATGGATCCTTTATCTACTGAAATGGTTGAGCAGACAGTTTTTGTAGTCTTCTTCTCAGGGTTGCAGGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTACCCTGTTCCTGAAGATATAACTAAGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAGCAACTTTGGATGCAGGGATTCGCCACAGAAATAAGGCCCTTACTTCTGTAGGGTTTCACCTGGCAAAATGGATGAACATGGTAAGAAGGGAGAAAGCTGTATATGATACATTGAACATGTTAAACTTTGATGTTACCAAAAAATGCCTTGTTGGAGAAGGCTGGTGTCCTATATTTGCAAAAACAAAGATTCAGGAGGCATTGCAACGTGCAACATTTGATAGCAATTCACAAGTGGGTGTAATATTTCATGTGATGGATGCAATAGAATCACCTCCTACATATTTTAGAACCAACAACTTCACAAGTGCATTTCAGGAAATTGTTGATGCATATGGCGTAGCAAGATATCAAGAAGCAAATCCTGCAGTGTACACTGTCATTACATTTCCATTTCTTTTCGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTGTTGATGGGAGCATTAGTTCTTATAGCCCGTGAAAGAAAGCTCAGTGCTCAGAAACTCGGGAGCTTTATGGAGATGCTATTTGGTGGGCGCTATGTACTTCTTTTAATGTCTCTGTTCTCAATTTACTGTGGGTTGATTTACAATGAGTTCTTCTCTGTCCCTTTTCACATATTTGGTGGGTCTGCTTACAAATGCAGAGATGCTTCTTGCAGTGATGCACACACTGTTGGCTTAATCAAGTACAGAGATCCATACCCATTTGGTGTGGATCCTAGTTGGCGTGGAAGTCGTTCAGAACTGCCCTTTTTGAATTCTCTCAAAATGAAATTGTCTATTTTGTTGGGTGTGGCGCAAATGAACGTAGGAATTATTCTCAGTTACTTCAATGCACGGTTTTTCAGCAGCTCAATTGATGTAAGGTACCAGTTTGTGCCGCAGATGATCTTCCTTAACAGTCTTTTTGGATATCTTTCACTTCTAGTTGTCATCAAGTGGTGCACCGGCTCTAAGGCTGACCTCTATCATGTGATGATTTACATGTTCTTAAGTCCAACTGATGATCTTGGGGAAAATCAGTTGTTTTGGGGCCAAAGACCACTTCAGATCATTTTGTTACTCTTGGCTCTAATTGCAGTCCCTTGGATGCTCTTTCCAAAACCTTTCATTTTGAGGAAGCGTCATACAGAGAGGTTTCAAGGTCGCACATATGGGATGCTTGGTACCTCTGAAATGGATCTTGACGTGGAAACTGATCCTGTGAGGCAACATCATGAGGAGTTTAATTTCAGTGAGGTGTTTGTGCACCAGATGATACACTCCATAGAGTTTGTTCTTGGTGCAGTTTCAAATACGGCGTCCTACCTTCGATTGTGGGCTTTGAGCTTGGCCCACTCAGAGTTGTCAACTGTGTTCTACGAGAAAGTCCTTCTCCTCGCCTGGGGGTATGACAGCTTTATCATCCGGTTAATTGGGTTAGCAGTTTTTGCCTTTGCAACTGCCTTCATATTGCTCATGATGGAGACACTCAGCGCTTTTCTTCATGCCTTGCGTCTTCATTGGGTGGAATTTCAAAATAAGTTCTACCTTGGTGATGGGTATAAGTTCAAACCTTTTTCCTTCACCTCCATAGCTACTGAGGATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

751

Amino Acids

85.79

Weight (kDa)

6.06

Isoelectric Point (pI)

40.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 1 - 742 1.3e-276 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1527, 1826
AccB1I GGYRCC 2 cut(s) 1527, 1826
AciI CCGC 1 cut(s) 1541
AclWI GGATC 8 cut(s) 36, 476, 489, 1349, 1370, 1383, 1848, 1853
AcsI RAATTY 4 cut(s) 364, 564, 1417, 2171
AcuI CTGAAG 2 cut(s) 624, 1691
AcyI GRCGYC 1 cut(s) 1961
AfaI GTAC 5 cut(s) 1040, 1203, 1349, 1529, 1828
AfeI AGCGCT 1 cut(s) 2137
AfiI CCNNNNNNNGG 1 cut(s) 2044
AflII CTTAAG 1 cut(s) 1651
AflIII ACRYGT 2 cut(s) 783, 1644
AjiI CACGTC 2 cut(s) 650, 1849
AjnI CCWGG 3 cut(s) 186, 726, 2042
AjuI GAANNNNNNNTTGG 2 cut(s) 1689, 1721
AloI GAACNNNNNNTCC 2 cut(s) 1107, 1139
Alw21I GWGCWC 5 cut(s) 123, 186, 1156, 1607, 1911
Alw26I GTCTC 2 cut(s) 1221, 2120
Alw44I GTGCAC 2 cut(s) 1603, 1907
AlwI GGATC 8 cut(s) 36, 476, 489, 1349, 1370, 1383, 1848, 1853
Ama87I CYCGRG 1 cut(s) 1163
Aor51HI AGCGCT 1 cut(s) 2137
AoxI GGCC 4 cut(s) 573, 702, 1693, 1992
ApaLI GTGCAC 2 cut(s) 1603, 1907
ApeKI GCWGC 2 cut(s) 13, 1509
ApoI RAATTY 4 cut(s) 364, 564, 1417, 2171
ArsI GACNNNNNNTTYG 2 cut(s) 1946, 1978
Asp700I GAANNNNTTC 2 cut(s) 162, 1396
Asp718I GGTACC 2 cut(s) 1527, 1826
AspLEI GCGC 4 cut(s) 18, 1197, 1459, 2138
AspS9I GGNCC 4 cut(s) 341, 703, 1693, 1993
AsuHPI GGTGA 5 cut(s) 128, 716, 930, 2207, 2218
AvaI CYCGRG 1 cut(s) 1163
AvaII GGWCC 1 cut(s) 341
BaeGI GKGCMC 2 cut(s) 1607, 1911
BamHI GGATCC 2 cut(s) 481, 1375
BanI GGYRCC 2 cut(s) 1527, 1826
BanII GRGCYC 1 cut(s) 186
BbsI GAAGAC 2 cut(s) 517, 2150
Bbv12I GWGCWC 5 cut(s) 123, 186, 1156, 1607, 1911
BbvI GCAGC 1 cut(s) 1521
BccI CCATC 6 cut(s) 193, 352, 916, 1105, 2116, 2192
BceAI ACGGC 1 cut(s) 1974
BciT130I CCWGG 3 cut(s) 188, 728, 2044
BcoDI GTCTC 2 cut(s) 1221, 2120
BfaI CTAG 4 cut(s) 269, 633, 1380, 1586
BfmI CTRYAG 2 cut(s) 714, 1032
BfoI RGCGCY 2 cut(s) 1198, 2139
BfrI CTTAAG 1 cut(s) 1651
BisI GCNGC 3 cut(s) 14, 1510, 1541
BlsI GCNGC 3 cut(s) 15, 1511, 1542
Bme1390I CCNGG 3 cut(s) 188, 728, 2044
Bme18I GGWCC 1 cut(s) 341
BmeT110I CYCGRG 1 cut(s) 1163
BmgBI CACGTC 2 cut(s) 650, 1849
BmgT120I GGNCC 4 cut(s) 341, 703, 1693, 1993
BmiI GGNNCC 6 cut(s) 342, 483, 1377, 1529, 1694, 1828
BmrFI CCNGG 3 cut(s) 188, 728, 2044
BmrI ACTGGG 1 cut(s) 1096
BmsI GCATC 9 cut(s) 405, 667, 916, 985, 1170, 1297, 1312, 1740, 1809
BmuI ACTGGG 1 cut(s) 1096
BoxI GACNNNNGTC 1 cut(s) 1089
BpiI GAAGAC 2 cut(s) 517, 2150
BplI GAGNNNNNCTC 2 cut(s) 1244, 1276
BpmI CTGGAG 2 cut(s) 209, 341
BsaHI GRCGYC 1 cut(s) 1961
BsaJI CCNNGG 3 cut(s) 1745, 2043, 2191
BsaWI WCCGGW 1 cut(s) 2067
BsaXI ACNNNNNCTCC 4 cut(s) 294, 324, 1107, 1137
Bsc4I CCNNNNNNNGG 1 cut(s) 2044
Bse118I RCCGGY 1 cut(s) 1607
Bse1I ACTGG 3 cut(s) 68, 1091, 1531
Bse3DI GCAATG 2 cut(s) 314, 866
BseBI CCWGG 3 cut(s) 188, 728, 2044
BseDI CCNNGG 3 cut(s) 1745, 2043, 2191
BseGI GGATG 8 cut(s) 420, 682, 744, 931, 1755, 1824, 2064, 2251
BseLI CCNNNNNNNGG 1 cut(s) 2044
BseMI GCAATG 2 cut(s) 314, 866
BseMII CTCAG 7 cut(s) 546, 1162, 1169, 1495, 2013, 2146, 2232
BseNI ACTGG 3 cut(s) 68, 1091, 1531
BseRI GAGGAG 2 cut(s) 1895, 2027
BseSI GKGCMC 2 cut(s) 1607, 1911
BseXI GCAGC 1 cut(s) 1521
BsgI GTGCAG 1 cut(s) 1965
BshFI GGCC 4 cut(s) 575, 704, 1695, 1994
BshNI GGYRCC 2 cut(s) 1527, 1826
BsiHKAI GWGCWC 5 cut(s) 123, 186, 1156, 1607, 1911
BsiHKCI CYCGRG 1 cut(s) 1163
BsiSI CCGG 2 cut(s) 1608, 2068
BslFI GGGAC 4 cut(s) 354, 1097, 1250, 1727
BslI CCNNNNNNNGG 1 cut(s) 2044
BsmAI GTCTC 2 cut(s) 1221, 2120
BsmFI GGGAC 4 cut(s) 354, 1097, 1250, 1727
BsnI GGCC 4 cut(s) 575, 704, 1695, 1994
BsoBI CYCGRG 1 cut(s) 1163
Bsp1286I GDGCHC 5 cut(s) 123, 186, 1156, 1607, 1911
Bsp1407I TGTACA 1 cut(s) 1038
BspACI CCGC 1 cut(s) 1541
BspANI GGCC 4 cut(s) 575, 704, 1695, 1994
BspCNI CTCAG 7 cut(s) 545, 1161, 1168, 1494, 2012, 2145, 2233
BspHI TCATGA 2 cut(s) 1876, 2118
BspLI GGNNCC 6 cut(s) 342, 483, 1377, 1529, 1694, 1828
BspMAI CTGCAG 1 cut(s) 1036
BspPI GGATC 8 cut(s) 36, 476, 489, 1349, 1370, 1383, 1848, 1853
BspT107I GGYRCC 2 cut(s) 1527, 1826
BspTI CTTAAG 1 cut(s) 1651
BsrDI GCAATG 2 cut(s) 314, 866
BsrFI RCCGGY 1 cut(s) 1607
BsrGI TGTACA 1 cut(s) 1038
BsrI ACTGG 3 cut(s) 68, 1091, 1531
BssAI RCCGGY 1 cut(s) 1607
BssECI CCNNGG 3 cut(s) 1745, 2043, 2191
BssNI GRCGYC 1 cut(s) 1961
BssT1I CCWWGG 2 cut(s) 1745, 2191
Bst2UI CCWGG 3 cut(s) 188, 728, 2044
Bst4CI ACNGT 7 cut(s) 514, 1045, 1235, 1333, 1500, 1562, 2014
Bst6I CTCTTC 1 cut(s) 267
BstACI GRCGYC 1 cut(s) 1961
BstAFI CTTAAG 1 cut(s) 1651
BstAPI GCANNNNNTGC 2 cut(s) 1031, 2090
BstAUI TGTACA 1 cut(s) 1038
BstC8I GCNNGC 1 cut(s) 543
BstF5I GGATG 8 cut(s) 420, 682, 744, 931, 1755, 1824, 2064, 2251
BstH2I RGCGCY 2 cut(s) 1198, 2139
BstHHI GCGC 4 cut(s) 18, 1197, 1459, 2138
BstMAI GTCTC 2 cut(s) 1221, 2120
BstMWI GCNNNNNNNGC 4 cut(s) 581, 1031, 1736, 2090
BstNI CCWGG 3 cut(s) 188, 728, 2044
BstNSI RCATGY 2 cut(s) 787, 1648
BstPAI GACNNNNGTC 1 cut(s) 1089
BstSCI CCNGG 3 cut(s) 186, 726, 2042
BstSFI CTRYAG 2 cut(s) 714, 1032
BstSLI GKGCMC 2 cut(s) 1607, 1911
BstV1I GCAGC 1 cut(s) 1521
BstV2I GAAGAC 2 cut(s) 517, 2150
BstX2I RGATCY 4 cut(s) 481, 1354, 1375, 1840
BstYI RGATCY 4 cut(s) 481, 1354, 1375, 1840
BsuRI GGCC 4 cut(s) 575, 704, 1695, 1994
BtrI CACGTC 2 cut(s) 650, 1849
BtsCI GGATG 8 cut(s) 420, 682, 744, 931, 1755, 1824, 2064, 2251
BtsI GCAGTG 2 cut(s) 1041, 1324
BtsIMutI CAGTG 7 cut(s) 315, 1041, 1041, 1156, 1324, 1329, 1900
Cac8I GCNNGC 1 cut(s) 543
CciI TCATGA 2 cut(s) 1876, 2118
CfoI GCGC 4 cut(s) 18, 1197, 1459, 2138
Cfr10I RCCGGY 1 cut(s) 1607
Cfr13I GGNCC 4 cut(s) 341, 703, 1693, 1993
CseI GACGC 3 cut(s) 1772, 1950, 2144
Csp6I GTAC 5 cut(s) 1039, 1202, 1348, 1528, 1827
CviQI GTAC 5 cut(s) 1039, 1202, 1348, 1528, 1827
Eam1104I CTCTTC 1 cut(s) 267
EarI CTCTTC 1 cut(s) 267
Ecl136II GAGCTC 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 1745, 2191
Eco147I AGGCCT 1 cut(s) 575
Eco24I GRGCYC 1 cut(s) 186
Eco32I GATATC 3 cut(s) 331, 1016, 1574
Eco47I GGWCC 1 cut(s) 341
Eco47III AGCGCT 1 cut(s) 2137
Eco53kI GAGCTC 1 cut(s) 184
Eco57I CTGAAG 2 cut(s) 624, 1691
Eco88I CYCGRG 1 cut(s) 1163
EcoICRI GAGCTC 1 cut(s) 184
EcoO109I RGGNCCY 1 cut(s) 703
EcoRI GAATTC 1 cut(s) 1417
EcoRII CCWGG 3 cut(s) 186, 726, 2042
EcoRV GATATC 3 cut(s) 331, 1016, 1574
EcoT14I CCWWGG 2 cut(s) 1745, 2191
EcoT22I ATGCAT 1 cut(s) 1000
EcoT38I GRGCYC 1 cut(s) 186
ErhI CCWWGG 2 cut(s) 1745, 2191
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 4 cut(s) 354, 1097, 1250, 1727
FauNDI CATATG 2 cut(s) 1000, 1813
Fnu4HI GCNGC 3 cut(s) 14, 1510, 1541
FokI GGATG 7 cut(s) 427, 689, 751, 938, 1762, 1831, 2051
FriOI GRGCYC 1 cut(s) 186
Fsp4HI GCNGC 3 cut(s) 14, 1510, 1541
FspBI CTAG 4 cut(s) 269, 633, 1380, 1586
GlaI GCGC 4 cut(s) 17, 1196, 1458, 2137
GluI GCNGC 3 cut(s) 14, 1510, 1541
GsuI CTGGAG 2 cut(s) 209, 341
HaeII RGCGCY 2 cut(s) 1198, 2139
HaeIII GGCC 4 cut(s) 575, 704, 1695, 1994
HapII CCGG 2 cut(s) 1608, 2068
HgaI GACGC 3 cut(s) 1772, 1950, 2144
HhaI GCGC 4 cut(s) 18, 1197, 1459, 2138
Hin1I GRCGYC 1 cut(s) 1961
Hin6I GCGC 4 cut(s) 16, 1195, 1457, 2136
HinP1I GCGC 4 cut(s) 16, 1195, 1457, 2136
HincII GTYRAC 1 cut(s) 2010
HindII GTYRAC 1 cut(s) 2010
HinfI GANTC 5 cut(s) 305, 556, 685, 856, 935
HpaII CCGG 2 cut(s) 1608, 2068
HphI GGTGA 5 cut(s) 128, 716, 930, 2207, 2218
Hpy166II GTNNAC 6 cut(s) 283, 1039, 1041, 1605, 1909, 2010
Hpy188I TCNGA 8 cut(s) 335, 349, 561, 1158, 1402, 1710, 1834, 2002
Hpy8I GTNNAC 6 cut(s) 283, 1039, 1041, 1605, 1909, 2010
HpyAV CCTTC 6 cut(s) 747, 818, 1979, 2042, 2116, 2233
HpyCH4III ACNGT 7 cut(s) 514, 1045, 1235, 1333, 1500, 1562, 2014
HpyCH4IV ACGT 5 cut(s) 64, 649, 874, 1467, 1848
HpyF10VI GCNNNNNNNGC 4 cut(s) 581, 1031, 1736, 2090
HpySE526I ACGT 5 cut(s) 64, 649, 874, 1467, 1848
Hsp92I GRCGYC 1 cut(s) 1961
HspAI GCGC 4 cut(s) 16, 1195, 1457, 2136
KpnI GGTACC 2 cut(s) 1531, 1830
LmnI GCTCC 4 cut(s) 189, 322, 1115, 1167
Lsp1109I GCAGC 1 cut(s) 1521
LweI GCATC 9 cut(s) 405, 667, 916, 985, 1170, 1297, 1312, 1740, 1809
MaeI CTAG 4 cut(s) 269, 633, 1380, 1586
MaeII ACGT 5 cut(s) 64, 649, 874, 1467, 1848
MaeIII GTNAC 4 cut(s) 250, 799, 1484, 1719
MboII GAAGA 8 cut(s) 284, 517, 520, 617, 1249, 1543, 2135, 2150
MfeI CAATTG 1 cut(s) 1515
MflI RGATCY 4 cut(s) 481, 1354, 1375, 1840
MhlI GDGCHC 5 cut(s) 123, 186, 1156, 1607, 1911
MmeI TCCRAC 3 cut(s) 72, 799, 1682
Mph1103I ATGCAT 1 cut(s) 1000
MroXI GAANNNNTTC 2 cut(s) 162, 1396
MseI TTAA 8 cut(s) 24, 788, 1211, 1340, 1557, 1652, 1887, 2072
MslI CAYNNNNRTG 1 cut(s) 126
MspCI CTTAAG 1 cut(s) 1651
MspI CCGG 2 cut(s) 1608, 2068
MspR9I CCNGG 3 cut(s) 188, 728, 2044
MunI CAATTG 1 cut(s) 1515
MvaI CCWGG 3 cut(s) 188, 728, 2044
MwoI GCNNNNNNNGC 4 cut(s) 581, 1031, 1736, 2090
NdeI CATATG 2 cut(s) 1000, 1813
NlaIV GGNNCC 6 cut(s) 342, 483, 1377, 1529, 1694, 1828
NmuCI GTSAC 1 cut(s) 250
NsiI ATGCAT 1 cut(s) 1000
NspI RCATGY 2 cut(s) 787, 1648
PagI TCATGA 2 cut(s) 1876, 2118
PceI AGGCCT 1 cut(s) 575
PciI ACATGT 2 cut(s) 783, 1644
PdmI GAANNNNTTC 2 cut(s) 162, 1396
PfeI GAWTC 5 cut(s) 305, 556, 685, 856, 935
PfoI TCCNGGA 1 cut(s) 186
PkrI GCNGC 3 cut(s) 15, 1511, 1542
PscI ACATGT 2 cut(s) 783, 1644
PshAI GACNNNNGTC 1 cut(s) 1089
Psp124BI GAGCTC 1 cut(s) 186
Psp6I CCWGG 3 cut(s) 186, 726, 2042
PspGI CCWGG 3 cut(s) 186, 726, 2042
PspN4I GGNNCC 6 cut(s) 342, 483, 1377, 1529, 1694, 1828
PspPI GGNCC 4 cut(s) 341, 703, 1693, 1993
PstI CTGCAG 1 cut(s) 1036
PsuI RGATCY 4 cut(s) 481, 1354, 1375, 1840
RsaI GTAC 5 cut(s) 1040, 1203, 1349, 1529, 1828
RsaNI GTAC 5 cut(s) 1039, 1202, 1348, 1528, 1827
RseI CAYNNNNRTG 1 cut(s) 126
SacI GAGCTC 1 cut(s) 186
SaqAI TTAA 8 cut(s) 24, 788, 1211, 1340, 1557, 1652, 1887, 2072
SatI GCNGC 3 cut(s) 14, 1510, 1541
Sau96I GGNCC 4 cut(s) 341, 703, 1693, 1993
ScrFI CCNGG 3 cut(s) 188, 728, 2044
SduI GDGCHC 5 cut(s) 123, 186, 1156, 1607, 1911
SfaNI GCATC 9 cut(s) 405, 667, 916, 985, 1170, 1297, 1312, 1740, 1809
SfcI CTRYAG 2 cut(s) 714, 1032
SinI GGWCC 1 cut(s) 341
SmiMI CAYNNNNRTG 1 cut(s) 126
SmlI CTYRAG 1 cut(s) 1651
SmoI CTYRAG 1 cut(s) 1651
SseBI AGGCCT 1 cut(s) 575
SsiI CCGC 1 cut(s) 1541
SspI AATATT 1 cut(s) 912
SspMI CTAG 4 cut(s) 269, 633, 1380, 1586
SstI GAGCTC 1 cut(s) 186
StuI AGGCCT 1 cut(s) 575
StyD4I CCNGG 3 cut(s) 186, 726, 2042
StyI CCWWGG 2 cut(s) 1745, 2191
TaaI ACNGT 7 cut(s) 514, 1045, 1235, 1333, 1500, 1562, 2014
TaiI ACGT 5 cut(s) 67, 652, 877, 1470, 1851
TaqI TCGA 2 cut(s) 166, 1972
TatI WGTACW 3 cut(s) 1038, 1201, 1347
TauI GCSGC 1 cut(s) 1543
TfiI GAWTC 5 cut(s) 305, 556, 685, 856, 935
Tru1I TTAA 8 cut(s) 24, 788, 1211, 1340, 1557, 1652, 1887, 2072
Tru9I TTAA 8 cut(s) 24, 788, 1211, 1340, 1557, 1652, 1887, 2072
TscAI CASTG 7 cut(s) 315, 1041, 1048, 1156, 1324, 1336, 1900
TseFI GTSAC 1 cut(s) 250
TseI GCWGC 2 cut(s) 13, 1509
Tsp45I GTSAC 1 cut(s) 250
TspRI CASTG 7 cut(s) 315, 1041, 1048, 1156, 1324, 1336, 1900
Vha464I CTTAAG 1 cut(s) 1651
VneI GTGCAC 2 cut(s) 1603, 1907
VpaK11BI GGWCC 1 cut(s) 341
XapI RAATTY 4 cut(s) 364, 564, 1417, 2171
XceI RCATGY 2 cut(s) 787, 1648
XmnI GAANNNNTTC 2 cut(s) 162, 1396
XspI CTAG 4 cut(s) 269, 633, 1380, 1586
Zsp2I ATGCAT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.