FvH4_3g33720

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009092
Physical Location & Seq
Forward (+)
29227319 .. 29239045
11727 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g33720.t7

Sequence Viewer

Length: 2379 bp
ATGGACAAGTTCCTCGACAAGTTGCCCTCCATGGATCTGATGCGCTCCGAGAAGATGACCTTCGTCCAGCTCATCATCCCCGTCGAGTCCGCCCACCGCACCGTTTCCTATCTCGGCGAACTCGGCCTCCTCCAATTCCGTGACTTAAATGCTGATAAGAGCCCTTTCCAGCTAACCTTTGTTAATCAGGTAAAGCGATGTGCAGAGATGTCAAGAAAGCTGCGCTTTTTTAAGGATCAAATCAGTAAAGCTGGTCTGCTTGGGTCTATACGTCCAGTTTCACAACCAGATATTGAGTTGGAGGAATTGGAGTTTCAACTTGGTGAGCACGAACATGAGCTGATTGAAATGAACTCTAATAGTGAAAGACTTCGACAATCATACAATGAGCTCCTGGAGTTCAAGATGGTATTGCAAAAGGCAAGCGGCTTTCTTGTTTCAAGTAATAGCCATGCTGTCACAGAGGAGACAGAACTAGAAGAGAATGTTTATTCAATGAATGACTATGGTGATTCAGTGTCACTGCTGGAGCAGGATATCAGACCGGGACCGTCAGATCAATCTGGTTTGAATTTTGTTAGTGGGATTATTTGTAAATCCAAAGCTCCAAGGTTTGAGAGGATGCTGTTTCGTGCTACCAGAGGAAATATGCTTTTTAACCAAGCTCCAGCAGATGAACAGATCATGGATCCTTTATCTACTGAAATGGTTGAGCAGACAGTTTTTGTAGTATTCTTCTCAGGGTTGCAGGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTACCCTGTTCCTGAAGATATTACAAAGCAGAGGCAAATAACTAGAGAAGTTTCATCTCGTCTTGCTGATTTGGAGGCAACATTGGATGCAGGGATTCGCCACAGAAATAAGGCCCTTACTTCTGTTGGGTTTCACCTGGCCAAATGGATGAATATGGTAAGAAGGGAGAAAGCTGTTTATGATACATTGAACATGTTAAACTTTGATGTTACCAAAAAATGCCTTGTCGGAGAAGGCTGGTGTCCAATATTTGCAAAAACAAAGATTCAGGAGGCATTGCAACGTGCAACATTTGATAGCAATTCACAAGAAATTGTTGATGCATACGGCGTAGCAAGATATCAAGAAGCAAATCCTGCAGTGTACACTGTTATCACATTTCCATTTCTTTTTGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTGTTGATGGGAGCATTAGTTCTTATAGCTCGTGAAAGAAAGCTCGCTGCTCAGAAACTCGGGAGCTTTATGGAGATGCTTTTTGGTGGGCGATATGTACTTCTTTTGATGTCTCTGTTCTCAATTTACTGTGGGTTGATTTACAATGAGTTCTTCTCTGTTCCTTTTCACATATTTGGTGGCTCCGCTTACAAATGCAGAGATGCTACTTGCAGTGATGCACACACTGTTGGATTAATCAAGTACCGAGATCCATACCCATTTGGTGTGGATCCCAGTTGGCGTGGAAGTCGTTCCGAACTGCCCTTCTTGAATTCTCTGAAAATGAAATTGTCTATTCTGTTGGGTGTGGTGCAAATGAACGTAGGAATTATGCTCAGTTACTTCAATGCACGGTTTTTCAACAGCTCAATTGATATAAGGTACCAGTTTGTGCCACAGATGATCTTCCTTAACAGTCTGTTCGGATATCTTTCGCTTCTAGTTGTCATCAAGTGGTGCACCGGCTCTAAGGCTGACCTCTATCATGTAATGATTTACATGTTCTTAAGTCCCACTGACGATCTTGGGGTAAATCAGTTGTTTTGGGGCCAAAGACCACTTCAGATTATTTTGTTAGTTTTGGCTCTAATTGCAGTTCCCTGGATGCTCTTTCCTAAACCTTTCATTTTGAGGAAGCTTCATACAGAGAGGTTTCAAGGTCGCACATATGGGATGCTTGGTACCTCTGAAATGGATCTTGACGTGGAAACTGATCCTGTGAGGCAACATCATGAGGAGTTTAATTTCAGTGAGGTGTTTGTGCACCAGATGATACATTCCATAGAGTTTGTTCTTGGTGCAGTTTCAAATACGGCGTCCTACCTTCGATTGTGGGCTTTGAGCTTGGCCCACTCAGAGTTGTCAACTGTTTTCTATGAGAAAGTCCTCCTACTCGCCTGGGGGTATGACAGCTTTATCATCCGGTTGATTGGATTAGCTGTTTTTGCCTTTGCAACTGCCTTCATATTGCTCATGATGGAGACACTCAGTGCTTTTCTTCATGCCTTGCGTCTTCATTGGGTGGAATTTCAAAATAAGTTCTACCTTGGTGATGGGTACAAGTTCAAACCTTTTTCCTTCGCCTCCTTAGCTACCGAGGATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

793

Amino Acids

90.46

Weight (kDa)

6.04

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 1 - 695 5.4e-269 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1650, 1949
AccB1I GGYRCC 2 cut(s) 1650, 1949
AciI CCGC 4 cut(s) 90, 97, 426, 1413
AclWI GGATC 9 cut(s) 42, 243, 683, 696, 1472, 1493, 1506, 1971, 1976
AcoI YGGCCR 1 cut(s) 936
AcsI RAATTY 4 cut(s) 571, 771, 1540, 2294
AcuI CTGAAG 2 cut(s) 831, 1814
AcyI GRCGYC 1 cut(s) 2084
AdeI CACNNNGTG 1 cut(s) 2258
AfaI GTAC 6 cut(s) 1163, 1326, 1472, 1652, 1951, 2327
AflII CTTAAG 1 cut(s) 1774
AflIII ACRYGT 2 cut(s) 990, 1767
AjiI CACGTC 1 cut(s) 1972
AjnI CCWGG 4 cut(s) 393, 933, 1868, 2165
AjuI GAANNNNNNNTTGG 2 cut(s) 1812, 1844
AloI GAACNNNNNNTCC 4 cut(s) 111, 143, 1230, 1262
Alw21I GWGCWC 4 cut(s) 330, 393, 1730, 2034
Alw26I GTCTC 3 cut(s) 461, 1344, 2243
Alw44I GTGCAC 2 cut(s) 1726, 2030
AlwI GGATC 9 cut(s) 42, 243, 683, 696, 1472, 1493, 1506, 1971, 1976
Ama87I CYCGRG 1 cut(s) 1286
AoxI GGCC 6 cut(s) 124, 780, 909, 936, 1816, 2115
ApaLI GTGCAC 2 cut(s) 1726, 2030
ApeKI GCWGC 2 cut(s) 220, 1274
ApoI RAATTY 4 cut(s) 571, 771, 1540, 2294
ArsI GACNNNNNNTTYG 2 cut(s) 2069, 2101
AseI ATTAAT 1 cut(s) 1463
Asp700I GAANNNNTTC 2 cut(s) 369, 1519
Asp718I GGTACC 2 cut(s) 1650, 1949
AspLEI GCGC 2 cut(s) 45, 225
AspS9I GGNCC 4 cut(s) 548, 910, 1816, 2116
AsuC2I CCSGG 1 cut(s) 546
AsuHPI GGTGA 4 cut(s) 335, 521, 923, 2330
AvaI CYCGRG 1 cut(s) 1286
AvaII GGWCC 1 cut(s) 548
BaeGI GKGCMC 2 cut(s) 1730, 2034
BalI TGGCCA 1 cut(s) 938
BamHI GGATCC 2 cut(s) 688, 1498
BanI GGYRCC 2 cut(s) 1650, 1949
BanII GRGCYC 2 cut(s) 164, 393
BauI CACGAG 1 cut(s) 1257
BbsI GAAGAC 1 cut(s) 2273
Bbv12I GWGCWC 4 cut(s) 330, 393, 1730, 2034
BbvI GCAGC 2 cut(s) 207, 1261
BccI CCATC 4 cut(s) 400, 1228, 2239, 2315
BceAI ACGGC 2 cut(s) 1141, 2097
BciT130I CCWGG 4 cut(s) 395, 935, 1870, 2167
BcnI CCSGG 1 cut(s) 546
BcoDI GTCTC 3 cut(s) 461, 1344, 2243
BfaI CTAG 3 cut(s) 476, 840, 1709
BfmI CTRYAG 1 cut(s) 1155
BfrI CTTAAG 1 cut(s) 1774
BisI GCNGC 3 cut(s) 221, 427, 1275
BlsI GCNGC 3 cut(s) 222, 428, 1276
Bme1390I CCNGG 5 cut(s) 395, 546, 935, 1870, 2167
Bme18I GGWCC 1 cut(s) 548
BmeT110I CYCGRG 1 cut(s) 1286
BmgBI CACGTC 1 cut(s) 1972
BmgT120I GGNCC 4 cut(s) 548, 910, 1816, 2116
BmiI GGNNCC 7 cut(s) 549, 690, 1411, 1500, 1652, 1817, 1951
BmrFI CCNGG 5 cut(s) 395, 546, 935, 1870, 2167
BmrI ACTGGG 2 cut(s) 1219, 1497
BmsI GCATC 9 cut(s) 30, 612, 874, 1108, 1293, 1420, 1435, 1863, 1932
BmuI ACTGGG 2 cut(s) 1219, 1497
BoxI GACNNNNGTC 2 cut(s) 62, 1212
BpiI GAAGAC 1 cut(s) 2273
BplI GAGNNNNNCTC 2 cut(s) 1367, 1399
BpmI CTGGAG 3 cut(s) 416, 548, 651
Bpu10I CCTNAGC 1 cut(s) 2356
BpuMI CCSGG 1 cut(s) 546
BsaHI GRCGYC 1 cut(s) 2084
BsaJI CCNNGG 6 cut(s) 30, 608, 1868, 2166, 2314, 2364
BsaWI WCCGGW 1 cut(s) 2190
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 1230, 1260
Bse118I RCCGGY 1 cut(s) 1730
Bse1I ACTGG 4 cut(s) 275, 1214, 1503, 1654
Bse3DI GCAATG 1 cut(s) 1073
BseBI CCWGG 4 cut(s) 395, 935, 1870, 2167
BseDI CCNNGG 6 cut(s) 30, 608, 1868, 2166, 2314, 2364
BseGI GGATG 8 cut(s) 75, 627, 889, 951, 1878, 1947, 2187, 2374
BseMI GCAATG 1 cut(s) 1073
BseMII CTCAG 5 cut(s) 753, 1292, 1618, 2136, 2269
BseNI ACTGG 4 cut(s) 275, 1214, 1503, 1654
BseRI GAGGAG 3 cut(s) 119, 479, 2018
BseSI GKGCMC 2 cut(s) 1730, 2034
BseXI GCAGC 2 cut(s) 207, 1261
BsgI GTGCAG 2 cut(s) 222, 2088
BshFI GGCC 6 cut(s) 126, 782, 911, 938, 1818, 2117
BshNI GGYRCC 2 cut(s) 1650, 1949
BsiHKAI GWGCWC 4 cut(s) 330, 393, 1730, 2034
BsiHKCI CYCGRG 1 cut(s) 1286
BsiSI CCGG 3 cut(s) 545, 1731, 2191
BslFI GGGAC 3 cut(s) 561, 1220, 1764
BsmAI GTCTC 3 cut(s) 461, 1344, 2243
BsmFI GGGAC 3 cut(s) 561, 1220, 1764
BsnI GGCC 6 cut(s) 126, 782, 911, 938, 1818, 2117
BsoBI CYCGRG 1 cut(s) 1286
Bsp1286I GDGCHC 5 cut(s) 164, 330, 393, 1730, 2034
Bsp1407I TGTACA 1 cut(s) 1161
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 4 cut(s) 90, 97, 426, 1413
BspANI GGCC 6 cut(s) 126, 782, 911, 938, 1818, 2117
BspCNI CTCAG 5 cut(s) 752, 1291, 1617, 2135, 2268
BspHI TCATGA 2 cut(s) 1999, 2241
BspLI GGNNCC 7 cut(s) 549, 690, 1411, 1500, 1652, 1817, 1951
BspMAI CTGCAG 1 cut(s) 1159
BspPI GGATC 9 cut(s) 42, 243, 683, 696, 1472, 1493, 1506, 1971, 1976
BspT107I GGYRCC 2 cut(s) 1650, 1949
BspTI CTTAAG 1 cut(s) 1774
BsrDI GCAATG 1 cut(s) 1073
BsrFI RCCGGY 1 cut(s) 1730
BsrGI TGTACA 1 cut(s) 1161
BsrI ACTGG 4 cut(s) 275, 1214, 1503, 1654
BssAI RCCGGY 1 cut(s) 1730
BssECI CCNNGG 6 cut(s) 30, 608, 1868, 2166, 2314, 2364
BssNI GRCGYC 1 cut(s) 2084
BssSI CACGAG 1 cut(s) 1257
BssT1I CCWWGG 3 cut(s) 30, 608, 2314
Bst2BI CACGAG 1 cut(s) 1257
Bst2UI CCWGG 4 cut(s) 395, 935, 1870, 2167
Bst4CI ACNGT 9 cut(s) 103, 552, 721, 1168, 1358, 1456, 1623, 1685, 2137
Bst6I CTCTTC 1 cut(s) 474
BstACI GRCGYC 1 cut(s) 2084
BstAFI CTTAAG 1 cut(s) 1774
BstAPI GCANNNNNTGC 1 cut(s) 1154
BstAUI TGTACA 1 cut(s) 1161
BstC8I GCNNGC 3 cut(s) 424, 750, 1272
BstDEI CTNAG 7 cut(s) 739, 1278, 1604, 1737, 2122, 2255, 2356
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 8 cut(s) 75, 627, 889, 951, 1878, 1947, 2187, 2374
BstHHI GCGC 2 cut(s) 45, 225
BstMAI GTCTC 3 cut(s) 461, 1344, 2243
BstMWI GCNNNNNNNGC 6 cut(s) 123, 788, 1154, 1859, 2213, 2357
BstNI CCWGG 4 cut(s) 395, 935, 1870, 2167
BstNSI RCATGY 2 cut(s) 994, 1771
BstPAI GACNNNNGTC 2 cut(s) 62, 1212
BstSCI CCNGG 5 cut(s) 393, 544, 933, 1868, 2165
BstSFI CTRYAG 1 cut(s) 1155
BstSLI GKGCMC 2 cut(s) 1730, 2034
BstV1I GCAGC 2 cut(s) 207, 1261
BstV2I GAAGAC 1 cut(s) 2273
BstX2I RGATCY 5 cut(s) 34, 688, 1477, 1498, 1963
BstYI RGATCY 5 cut(s) 34, 688, 1477, 1498, 1963
BsuRI GGCC 6 cut(s) 126, 782, 911, 938, 1818, 2117
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 1972
BtsCI GGATG 8 cut(s) 75, 627, 889, 951, 1878, 1947, 2187, 2374
BtsI GCAGTG 3 cut(s) 521, 1164, 1447
BtsIMutI CAGTG 9 cut(s) 521, 522, 1164, 1164, 1447, 1452, 1782, 2023, 2263
Cac8I GCNNGC 3 cut(s) 424, 750, 1272
CciI TCATGA 2 cut(s) 1999, 2241
CfoI GCGC 2 cut(s) 45, 225
Cfr10I RCCGGY 1 cut(s) 1730
Cfr13I GGNCC 4 cut(s) 548, 910, 1816, 2116
CseI GACGC 2 cut(s) 2073, 2267
Csp6I GTAC 6 cut(s) 1162, 1325, 1471, 1651, 1950, 2326
CviQI GTAC 6 cut(s) 1162, 1325, 1471, 1651, 1950, 2326
DdeI CTNAG 7 cut(s) 739, 1278, 1604, 1737, 2122, 2255, 2356
DraIII CACNNNGTG 1 cut(s) 2258
EaeI YGGCCR 1 cut(s) 936
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 3 cut(s) 30, 608, 2314
Eco147I AGGCCT 1 cut(s) 782
Eco24I GRGCYC 2 cut(s) 164, 393
Eco32I GATATC 3 cut(s) 538, 1139, 1697
Eco47I GGWCC 1 cut(s) 548
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 2 cut(s) 831, 1814
Eco88I CYCGRG 1 cut(s) 1286
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 1 cut(s) 1540
EcoRII CCWGG 4 cut(s) 393, 933, 1868, 2165
EcoRV GATATC 3 cut(s) 538, 1139, 1697
EcoT14I CCWWGG 3 cut(s) 30, 608, 2314
EcoT22I ATGCAT 1 cut(s) 1123
EcoT38I GRGCYC 2 cut(s) 164, 393
ErhI CCWWGG 3 cut(s) 30, 608, 2314
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 209, 241
FaqI GGGAC 3 cut(s) 561, 1220, 1764
FauNDI CATATG 1 cut(s) 1936
Fnu4HI GCNGC 3 cut(s) 221, 427, 1275
FokI GGATG 7 cut(s) 62, 634, 896, 958, 1885, 1954, 2174
FriOI GRGCYC 2 cut(s) 164, 393
Fsp4HI GCNGC 3 cut(s) 221, 427, 1275
FspBI CTAG 3 cut(s) 476, 840, 1709
GlaI GCGC 2 cut(s) 44, 224
GluI GCNGC 3 cut(s) 221, 427, 1275
GsuI CTGGAG 3 cut(s) 416, 548, 651
HaeIII GGCC 6 cut(s) 126, 782, 911, 938, 1818, 2117
HapII CCGG 3 cut(s) 545, 1731, 2191
HgaI GACGC 2 cut(s) 2073, 2267
HhaI GCGC 2 cut(s) 45, 225
Hin1I GRCGYC 1 cut(s) 2084
Hin6I GCGC 2 cut(s) 43, 223
HinP1I GCGC 2 cut(s) 43, 223
HincII GTYRAC 1 cut(s) 2133
HindII GTYRAC 1 cut(s) 2133
HindIII AAGCTT 1 cut(s) 1904
HinfI GANTC 5 cut(s) 86, 512, 763, 892, 1063
HpaII CCGG 3 cut(s) 545, 1731, 2191
HphI GGTGA 4 cut(s) 335, 521, 923, 2330
Hpy166II GTNNAC 5 cut(s) 1162, 1164, 1728, 2032, 2133
Hpy8I GTNNAC 5 cut(s) 1162, 1164, 1728, 2032, 2133
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 7 cut(s) 70, 954, 1025, 1543, 2102, 2239, 2356
HpyCH4III ACNGT 9 cut(s) 103, 552, 721, 1168, 1358, 1456, 1623, 1685, 2137
HpyCH4IV ACGT 4 cut(s) 271, 1081, 1590, 1971
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 788, 1154, 1859, 2213, 2357
HpyF3I CTNAG 7 cut(s) 739, 1278, 1604, 1737, 2122, 2255, 2356
HpySE526I ACGT 4 cut(s) 271, 1081, 1590, 1971
Hsp92I GRCGYC 1 cut(s) 2084
HspAI GCGC 2 cut(s) 43, 223
KpnI GGTACC 2 cut(s) 1654, 1953
LmnI GCTCC 8 cut(s) 50, 396, 529, 610, 670, 1238, 1290, 1415
Lsp1109I GCAGC 2 cut(s) 207, 1261
LweI GCATC 9 cut(s) 30, 612, 874, 1108, 1293, 1420, 1435, 1863, 1932
MaeI CTAG 3 cut(s) 476, 840, 1709
MaeII ACGT 4 cut(s) 271, 1081, 1590, 1971
MaeIII GTNAC 5 cut(s) 140, 457, 519, 1006, 1607
MboII GAAGA 8 cut(s) 64, 491, 727, 824, 1372, 1666, 2258, 2273
MfeI CAATTG 1 cut(s) 1638
MflI RGATCY 5 cut(s) 34, 688, 1477, 1498, 1963
MhlI GDGCHC 5 cut(s) 164, 330, 393, 1730, 2034
MlsI TGGCCA 1 cut(s) 938
MluNI TGGCCA 1 cut(s) 938
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 279, 1006, 1438
Mox20I TGGCCA 1 cut(s) 938
Mph1103I ATGCAT 1 cut(s) 1123
MroXI GAANNNNTTC 2 cut(s) 369, 1519
MscI TGGCCA 1 cut(s) 938
MseI TTAA 9 cut(s) 146, 183, 231, 657, 995, 1463, 1680, 1775, 2010
MslI CAYNNNNRTG 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 938
MspCI CTTAAG 1 cut(s) 1774
MspI CCGG 3 cut(s) 545, 1731, 2191
MspR9I CCNGG 5 cut(s) 395, 546, 935, 1870, 2167
MunI CAATTG 1 cut(s) 1638
MvaI CCWGG 4 cut(s) 395, 935, 1870, 2167
MwoI GCNNNNNNNGC 6 cut(s) 123, 788, 1154, 1859, 2213, 2357
NciI CCSGG 1 cut(s) 546
NcoI CCATGG 1 cut(s) 30
NdeI CATATG 1 cut(s) 1936
NlaIV GGNNCC 7 cut(s) 549, 690, 1411, 1500, 1652, 1817, 1951
NmeAIII GCCGAG 2 cut(s) 93, 102
NmuCI GTSAC 3 cut(s) 140, 457, 519
NsiI ATGCAT 1 cut(s) 1123
NspI RCATGY 2 cut(s) 994, 1771
PagI TCATGA 2 cut(s) 1999, 2241
PceI AGGCCT 1 cut(s) 782
PciI ACATGT 2 cut(s) 990, 1767
PdmI GAANNNNTTC 2 cut(s) 369, 1519
PfeI GAWTC 4 cut(s) 512, 763, 892, 1063
PfoI TCCNGGA 1 cut(s) 393
PkrI GCNGC 3 cut(s) 222, 428, 1276
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PscI ACATGT 2 cut(s) 990, 1767
PshAI GACNNNNGTC 2 cut(s) 62, 1212
PshBI ATTAAT 1 cut(s) 1463
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 4 cut(s) 393, 933, 1868, 2165
PspGI CCWGG 4 cut(s) 393, 933, 1868, 2165
PspN4I GGNNCC 7 cut(s) 549, 690, 1411, 1500, 1652, 1817, 1951
PspPI GGNCC 4 cut(s) 548, 910, 1816, 2116
PstI CTGCAG 1 cut(s) 1159
PsuI RGATCY 5 cut(s) 34, 688, 1477, 1498, 1963
RsaI GTAC 6 cut(s) 1163, 1326, 1472, 1652, 1951, 2327
RsaNI GTAC 6 cut(s) 1162, 1325, 1471, 1651, 1950, 2326
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 1 cut(s) 393
SaqAI TTAA 9 cut(s) 146, 183, 231, 657, 995, 1463, 1680, 1775, 2010
SatI GCNGC 3 cut(s) 221, 427, 1275
Sau96I GGNCC 4 cut(s) 548, 910, 1816, 2116
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 5 cut(s) 395, 546, 935, 1870, 2167
SduI GDGCHC 5 cut(s) 164, 330, 393, 1730, 2034
SfaNI GCATC 9 cut(s) 30, 612, 874, 1108, 1293, 1420, 1435, 1863, 1932
SfcI CTRYAG 1 cut(s) 1155
SinI GGWCC 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 1774
SmoI CTYRAG 1 cut(s) 1774
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 4 cut(s) 90, 97, 426, 1413
SspI AATATT 1 cut(s) 1047
SspMI CTAG 3 cut(s) 476, 840, 1709
SstI GAGCTC 1 cut(s) 393
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 5 cut(s) 393, 544, 933, 1868, 2165
StyI CCWWGG 3 cut(s) 30, 608, 2314
TaaI ACNGT 9 cut(s) 103, 552, 721, 1168, 1358, 1456, 1623, 1685, 2137
TaiI ACGT 4 cut(s) 274, 1084, 1593, 1974
TaqI TCGA 4 cut(s) 15, 84, 373, 2095
TatI WGTACW 2 cut(s) 1161, 1324
TauI GCSGC 1 cut(s) 429
TfiI GAWTC 4 cut(s) 512, 763, 892, 1063
Tru1I TTAA 9 cut(s) 146, 183, 231, 657, 995, 1463, 1680, 1775, 2010
Tru9I TTAA 9 cut(s) 146, 183, 231, 657, 995, 1463, 1680, 1775, 2010
TscAI CASTG 9 cut(s) 522, 528, 1164, 1171, 1447, 1459, 1789, 2023, 2263
TseFI GTSAC 3 cut(s) 140, 457, 519
TseI GCWGC 2 cut(s) 220, 1274
Tsp45I GTSAC 3 cut(s) 140, 457, 519
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 9 cut(s) 522, 528, 1164, 1171, 1447, 1459, 1789, 2023, 2263
Vha464I CTTAAG 1 cut(s) 1774
VneI GTGCAC 2 cut(s) 1726, 2030
VpaK11BI GGWCC 1 cut(s) 548
VspI ATTAAT 1 cut(s) 1463
XapI RAATTY 4 cut(s) 571, 771, 1540, 2294
XceI RCATGY 2 cut(s) 994, 1771
XmnI GAANNNNTTC 2 cut(s) 369, 1519
XspI CTAG 3 cut(s) 476, 840, 1709
Zsp2I ATGCAT 1 cut(s) 1123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.