AT2G28520

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009092
Physical Location & Seq
Forward (+)
12209800 .. 12216077
6278 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G28520.1

Sequence Viewer

Length: 2454 bp
ATGGAGGAATTCTTAGATAAGTTGCCGCAGATGGATCTGATGCGTTCGGAGAAGATGACGCTTGTTCAGCTTATCATCCCTGTTGAATCCGCTCATCGTTCCATCACTTATCTCGGCGAGCTCGGACTCTTGCAGTTTCGCGACTTAAATGCTGATAAGAGTCCCTTTCAACGGACATTTGCAAATCAGGTAAAACGATGTGGTGAGATGTCAAGAAAGCTGCGGTTTTTTAAAGATCAAATTGACAAAGCTGGTCTAAGATGTTCACCACGCCTTGAAATAGAACCAGATATTGCACTTGGAGATTTAGAGAGACAACTTGCTGATCACGAGCATGAAGTACTTGAAATGAATTCAAACAGTGAAAAGCTTCGGCAGACATATAACGAACTTCTAGAATTCAAGATAGTTCTTGAAAAGGCAAGTGGTTTCCTTGTCTCAAGTAATACTCATGCAATTGGAGAAGAAATTGAACTACATGAAAGCACCTACTCGAATAACGGTTTTATTGAGACTGCCTCTTTACTTGAGCAGGAAATGAATCCTGGACACTCTAATCAATCAGGATTGAGATTTATTAGTGGAATCATCAACAAAGACAAACTTCTTAAGTTTGAAAGAATGTTGTTTCGTGCAACACGAGGCAATATGCTTTTCAACCAAACAACCTCTGATGAGGAAATCATGGATCCTTCAACATCAGAAATGGTGGAGAAAGTTGTATTTGTGGTATTCTTCTCTGGGGAGCAAGCAAGAACAAAAATATTGAAAATATGTGAAGCATTTGGTGCAAATTGCTATCCTGTCCCCGAGGATACAACTAAGCAGAGGCAGCTTACTAGAGAAGTTTTATCCCGACTCTCTGATCTGGAAGCCACATTAGATGCTGGAACTCGCCATCGGAATAATGCCCTTAATTCTGTTGGCTATAGCTTAACTAACTGGATCACCACGGTTCGGAGAGAAAAAGCTGTTTATGATACTCTGAATATGCTAAATTTTGATGTAACCAAGAAATGCCTTGTTGGCGAAGGTTGGTGCCCCACATTTGCTAAGACCCAGATTCATGAGGTCCTGCAGCGAGCAACTTTCGACAGCAGTTCACAGGTGGGCGTTATATTTCATGTAATGCAAGCAGTAGAATCACCTCCCACCTATTTCAGAACGAACAAACTTACAAATGCCTTCCAGGAGATTATTGATGCTTACGGTGTTGCGAGATATCAAGAGGCAAACCCTGCTGTTTATTCAGTTGTCACATATCCCTTTTTGTTTGCTGTTATGTTTGGTGATTGGGGTCATGGTCTATGCTTGCTGCTAGGAGCGTTGTATCTTCTTGCTCGTGAAAGAAAGTTGAGTACGCAGAAACTTGGAAGCTTTATGGAGATGCTTTTTGGAGGCCGCTATGTAATCTTACTAATGGCACTTTTTTCAATATATTGTGGGCTTATCTACAATGAGTTCTTCTCTGTTCCTTTCCACATCTTTGGTGGTTCAGCTTACAAATGCAGAGATACAACTTGTAGCGATGCTTACACGGTTGGTTTAATCAAGTACCGTGATCCATACCCTTTTGGCGTAGATCCTAGTTGGCGTGGCAGTCGTACAGAACTGCCTTACCTAAACTCTCTTAAGATGAAGATGTCTATTCTGCTGGGAATTGCTCAGATGAATCTGGGACTAATATTAAGTTTCTTTAATGCCCGCTTTTTCGGCTCATCATTGGACATAAGGTATCAGTTTATACCTCAGATGATATTTCTGAACAGCTTATTTGGTTACCTTTCACTGCTCATCATCATCAAGTGGTGCACTGGATCTCAAGCAGACTTGTATCATGTAATGATCTACATGTTTCTGAGTCCCACCGAAGAACTTGGAGAAAACGAGTTGTTCTGGGGCCAGCGTCCACTTCAGATTGTGTTATTGCTTCTGGCATTCATCGCTGTCCCATGGATGCTTTTTCCGAAACCTTTTGCCCTCAGGAAAATTCACATGGAGAGGTTTCAAGGTCGCACGTACGGGGTTCTTGTTTCTTCTGAGGTGGATCTTGATGTAGAACCAGACTCCGCAAGAGGAGGAGGCCATCACGAAGAAGAGTTTAACTTCAGCGAGATTTTTGTACATCAATTGATTCACTCCATAGAATTTGTTCTAGGTTCAGTTTCTAACACAGCATCTTACCTTCGTCTTTGGGCTCTTAGTTTGGCTCATTCGGAATTGTCAACAGTCTTCTATGAGAAAGTTCTTCTTCTTGCCTGGGGGTATGAAAACATTTTGATCCGGTTAATCGGAGTAGCGGTGTTTGCGTTTGCAACGGCGTTTATACTTCTGATGATGGAAACACTAAGTGCATTTCTCCATGCTCTGCGTCTTCACTGGGTAGAATTCATGGGGAAGTTCTTCAATGGAGATGGTTACAAGTTCAAGCCTTTCTCTTTCGCTTTAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

817

Amino Acids

93.41

Weight (kDa)

5.98

Isoelectric Point (pI)

35.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 37 - 814 4.2e-293 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1038
AccBSI CCGCTC 1 cut(s) 92
AccII CGCG 1 cut(s) 141
AciI CCGC 7 cut(s) 26, 90, 223, 1402, 1705, 2070, 2300
AclWI GGATC 9 cut(s) 42, 683, 696, 953, 1556, 1577, 1825, 2055, 2275
AcsI RAATTY 7 cut(s) 8, 352, 398, 997, 1989, 2147, 2387
AcuI CTGAAG 2 cut(s) 1898, 2092
AdeI CACNNNGTG 1 cut(s) 2351
AfaI GTAC 6 cut(s) 342, 1360, 1556, 1606, 2021, 2124
AfiI CCNNNNNNNGG 2 cut(s) 171, 957
AflII CTTAAG 2 cut(s) 608, 1631
AflIII ACRYGT 1 cut(s) 1851
AjnI CCWGG 3 cut(s) 544, 1188, 2258
Alw21I GWGCWC 2 cut(s) 123, 1814
Alw26I GTCTC 3 cut(s) 307, 442, 506
Alw44I GTGCAC 1 cut(s) 1810
AlwI GGATC 9 cut(s) 42, 683, 696, 953, 1556, 1577, 1825, 2055, 2275
Ama87I CYCGRG 1 cut(s) 809
AoxI GGCC 3 cut(s) 1399, 1900, 2083
ApaLI GTGCAC 1 cut(s) 1810
ApeKI GCWGC 4 cut(s) 220, 832, 1078, 1315
ApoI RAATTY 7 cut(s) 8, 352, 398, 997, 1989, 2147, 2387
Asp700I GAANNNNTTC 3 cut(s) 369, 2151, 2402
AspS9I GGNCC 2 cut(s) 1072, 1900
AsuHPI GGTGA 5 cut(s) 215, 258, 940, 1137, 1301
AvaI CYCGRG 1 cut(s) 809
AvaII GGWCC 1 cut(s) 1072
AxyI CCTNAGG 1 cut(s) 1982
BaeGI GKGCMC 2 cut(s) 1043, 1814
BamHI GGATCC 1 cut(s) 688
BanI GGYRCC 1 cut(s) 1038
BanII GRGCYC 2 cut(s) 123, 2200
BauI CACGAG 3 cut(s) 329, 639, 1341
BbsI GAAGAC 2 cut(s) 2224, 2366
Bbv12I GWGCWC 2 cut(s) 123, 1814
BbvI GCAGC 4 cut(s) 207, 844, 1090, 1302
BccI CCATC 6 cut(s) 25, 110, 906, 2094, 2332, 2408
BceAI ACGGC 1 cut(s) 2334
BcgI CGANNNNNNTGC 2 cut(s) 131, 165
BciT130I CCWGG 3 cut(s) 546, 1190, 2260
BciVI GTATCC 1 cut(s) 808
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 3 cut(s) 307, 442, 506
BfaI CTAG 5 cut(s) 395, 840, 1319, 1587, 2156
BfmI CTRYAG 2 cut(s) 928, 1076
BfrI CTTAAG 2 cut(s) 608, 1631
BfuI GTATCC 1 cut(s) 808
BglI GCCNNNNNGGC 1 cut(s) 1026
BisI GCNGC 6 cut(s) 26, 221, 833, 1079, 1316, 1402
BlsI GCNGC 6 cut(s) 27, 222, 834, 1080, 1317, 1403
BmcAI AGTACT 1 cut(s) 342
Bme1390I CCNGG 3 cut(s) 546, 1190, 2260
Bme18I GGWCC 1 cut(s) 1072
BmeT110I CYCGRG 1 cut(s) 809
BmgT120I GGNCC 2 cut(s) 1072, 1900
BmiI GGNNCC 3 cut(s) 690, 1040, 1901
BmrFI CCNGG 3 cut(s) 546, 1190, 2260
BmrI ACTGGG 1 cut(s) 2389
BmsI GCATC 7 cut(s) 30, 874, 1192, 1377, 1519, 1947, 2186
BmuI ACTGGG 1 cut(s) 2389
BpiI GAAGAC 2 cut(s) 2224, 2366
BplI GAGNNNNNCTC 4 cut(s) 503, 535, 1451, 1483
BpuEI CTTGAG 3 cut(s) 424, 548, 1806
BsaAI YACGTR 1 cut(s) 2019
BsaJI CCNNGG 4 cut(s) 810, 951, 1952, 2259
BsaWI WCCGGW 1 cut(s) 2283
BsaXI ACNNNNNCTCC 2 cut(s) 2286, 2316
Bsc4I CCNNNNNNNGG 2 cut(s) 171, 957
Bse1I ACTGG 3 cut(s) 947, 1819, 2384
Bse21I CCTNAGG 1 cut(s) 1982
BseBI CCWGG 3 cut(s) 546, 1190, 2260
BseDI CCNNGG 4 cut(s) 810, 951, 1952, 2259
BseGI GGATG 2 cut(s) 75, 1962
BseLI CCNNNNNNNGG 2 cut(s) 171, 957
BseMII CTCAG 5 cut(s) 1679, 1763, 1850, 1996, 2031
BseNI ACTGG 3 cut(s) 947, 1819, 2384
BseRI GAGGAG 2 cut(s) 2091, 2094
BseSI GKGCMC 2 cut(s) 1043, 1814
BseXI GCAGC 4 cut(s) 207, 844, 1090, 1302
BseYI CCCAGC 1 cut(s) 1654
Bsh1236I CGCG 1 cut(s) 141
BshFI GGCC 3 cut(s) 1401, 1902, 2085
BshNI GGYRCC 1 cut(s) 1038
BsiHKAI GWGCWC 2 cut(s) 123, 1814
BsiHKCI CYCGRG 1 cut(s) 809
BsiSI CCGG 1 cut(s) 2284
BsiWI CGTACG 1 cut(s) 2019
BslFI GGGAC 5 cut(s) 147, 791, 1692, 1848, 1934
BslI CCNNNNNNNGG 2 cut(s) 171, 957
BsmAI GTCTC 3 cut(s) 307, 442, 506
BsmFI GGGAC 5 cut(s) 147, 791, 1692, 1848, 1934
BsmI GAATGC 1 cut(s) 1937
BsnI GGCC 3 cut(s) 1401, 1902, 2085
BsoBI CYCGRG 1 cut(s) 809
Bsp1286I GDGCHC 4 cut(s) 123, 1043, 1814, 2200
Bsp1407I TGTACA 1 cut(s) 2122
Bsp19I CCATGG 1 cut(s) 1952
Bsp68I TCGCGA 1 cut(s) 141
BspACI CCGC 7 cut(s) 26, 90, 223, 1402, 1705, 2070, 2300
BspANI GGCC 3 cut(s) 1401, 1902, 2085
BspCNI CTCAG 5 cut(s) 1678, 1762, 1851, 1995, 2032
BspFNI CGCG 1 cut(s) 141
BspHI TCATGA 1 cut(s) 1066
BspLI GGNNCC 3 cut(s) 690, 1040, 1901
BspMAI CTGCAG 1 cut(s) 1080
BspPI GGATC 9 cut(s) 42, 683, 696, 953, 1556, 1577, 1825, 2055, 2275
BspT107I GGYRCC 1 cut(s) 1038
BspTI CTTAAG 2 cut(s) 608, 1631
BsrBI CCGCTC 1 cut(s) 92
BsrGI TGTACA 1 cut(s) 2122
BsrI ACTGG 3 cut(s) 947, 1819, 2384
BssECI CCNNGG 4 cut(s) 810, 951, 1952, 2259
BssSI CACGAG 3 cut(s) 329, 639, 1341
BssT1I CCWWGG 1 cut(s) 1952
Bst2BI CACGAG 3 cut(s) 329, 639, 1341
Bst2UI CCWGG 3 cut(s) 546, 1190, 2260
Bst4CI ACNGT 7 cut(s) 362, 503, 955, 1211, 1540, 1559, 2230
Bst6I CTCTTC 1 cut(s) 2091
BstAFI CTTAAG 2 cut(s) 608, 1631
BstAPI GCANNNNNTGC 2 cut(s) 788, 1238
BstAUI TGTACA 1 cut(s) 2122
BstBAI YACGTR 1 cut(s) 2019
BstC8I GCNNGC 7 cut(s) 119, 750, 1083, 1134, 1313, 1705, 1904
BstDSI CCRYGG 2 cut(s) 951, 1952
BstEII GGTNACC 1 cut(s) 1778
BstF5I GGATG 2 cut(s) 75, 1962
BstFNI CGCG 1 cut(s) 141
BstMAI GTCTC 3 cut(s) 307, 442, 506
BstMWI GCNNNNNNNGC 8 cut(s) 67, 788, 832, 1026, 1238, 1713, 1943, 2306
BstNI CCWGG 3 cut(s) 546, 1190, 2260
BstNSI RCATGY 1 cut(s) 1855
BstPI GGTNACC 1 cut(s) 1778
BstSCI CCNGG 3 cut(s) 544, 1188, 2258
BstSFI CTRYAG 2 cut(s) 928, 1076
BstSLI GKGCMC 2 cut(s) 1043, 1814
BstUI CGCG 1 cut(s) 141
BstV1I GCAGC 4 cut(s) 207, 844, 1090, 1302
BstV2I GAAGAC 2 cut(s) 2224, 2366
BstX2I RGATCY 5 cut(s) 34, 688, 1582, 1817, 2047
BstXI CCANNNNNNTGG 1 cut(s) 1487
BstYI RGATCY 5 cut(s) 34, 688, 1582, 1817, 2047
Bsu36I CCTNAGG 1 cut(s) 1982
BsuI GTATCC 1 cut(s) 808
BsuRI GGCC 3 cut(s) 1401, 1902, 2085
BtgI CCRYGG 2 cut(s) 951, 1952
BtgZI GCGATG 2 cut(s) 1542, 1927
BtsCI GGATG 2 cut(s) 75, 1962
BtsI GCAGTG 1 cut(s) 1787
BtsIMutI CAGTG 4 cut(s) 367, 1787, 1812, 2377
BtuMI TCGCGA 1 cut(s) 141
Cac8I GCNNGC 7 cut(s) 119, 750, 1083, 1134, 1313, 1705, 1904
CciI TCATGA 1 cut(s) 1066
Cfr13I GGNCC 2 cut(s) 1072, 1900
CseI GACGC 3 cut(s) 67, 1895, 2360
Csp6I GTAC 6 cut(s) 341, 1359, 1555, 1605, 2020, 2123
CviQI GTAC 6 cut(s) 341, 1359, 1555, 1605, 2020, 2123
DraI TTTAAA 1 cut(s) 232
DraIII CACNNNGTG 1 cut(s) 2351
Eam1104I CTCTTC 1 cut(s) 2091
EarI CTCTTC 1 cut(s) 2091
Ecl136II GAGCTC 1 cut(s) 121
Eco130I CCWWGG 1 cut(s) 1952
Eco24I GRGCYC 2 cut(s) 123, 2200
Eco32I GATATC 1 cut(s) 1223
Eco47I GGWCC 1 cut(s) 1072
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 2 cut(s) 1898, 2092
Eco81I CCTNAGG 1 cut(s) 1982
Eco88I CYCGRG 1 cut(s) 809
Eco91I GGTNACC 1 cut(s) 1778
EcoICRI GAGCTC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 1072
EcoO65I GGTNACC 1 cut(s) 1778
EcoRI GAATTC 4 cut(s) 8, 352, 398, 2387
EcoRII CCWGG 3 cut(s) 544, 1188, 2258
EcoRV GATATC 1 cut(s) 1223
EcoT14I CCWWGG 1 cut(s) 1952
EcoT38I GRGCYC 2 cut(s) 123, 2200
ErhI CCWWGG 1 cut(s) 1952
FalI AAGNNNNNCTT 6 cut(s) 149, 181, 588, 620, 2235, 2267
FaqI GGGAC 5 cut(s) 147, 791, 1692, 1848, 1934
FauI CCCGC 1 cut(s) 1712
FbaI TGATCA 1 cut(s) 325
Fnu4HI GCNGC 6 cut(s) 26, 221, 833, 1079, 1316, 1402
FokI GGATG 2 cut(s) 62, 1969
FriOI GRGCYC 2 cut(s) 123, 2200
Fsp4HI GCNGC 6 cut(s) 26, 221, 833, 1079, 1316, 1402
FspBI CTAG 5 cut(s) 395, 840, 1319, 1587, 2156
GluI GCNGC 6 cut(s) 26, 221, 833, 1079, 1316, 1402
GsaI CCCAGC 1 cut(s) 1658
HaeIII GGCC 3 cut(s) 1401, 1902, 2085
HapII CCGG 1 cut(s) 2284
HgaI GACGC 3 cut(s) 67, 1895, 2360
HincII GTYRAC 1 cut(s) 2226
HindII GTYRAC 1 cut(s) 2226
HindIII AAGCTT 2 cut(s) 368, 1375
HpaII CCGG 1 cut(s) 2284
HphI GGTGA 5 cut(s) 215, 258, 940, 1137, 1301
Hpy166II GTNNAC 5 cut(s) 266, 1103, 1812, 1910, 2226
Hpy8I GTNNAC 5 cut(s) 266, 1103, 1812, 1910, 2226
HpyAV CCTTC 4 cut(s) 702, 1025, 1195, 2195
HpyCH4III ACNGT 7 cut(s) 362, 503, 955, 1211, 1540, 1559, 2230
HpyCH4IV ACGT 1 cut(s) 2018
HpyF10VI GCNNNNNNNGC 8 cut(s) 67, 788, 832, 1026, 1238, 1713, 1943, 2306
HpySE526I ACGT 1 cut(s) 2018
Ksp22I TGATCA 1 cut(s) 325
LmnI GCTCC 2 cut(s) 745, 1322
Lsp1109I GCAGC 4 cut(s) 207, 844, 1090, 1302
LweI GCATC 7 cut(s) 30, 874, 1192, 1377, 1519, 1947, 2186
MaeI CTAG 5 cut(s) 395, 840, 1319, 1587, 2156
MaeII ACGT 1 cut(s) 2018
MaeIII GTNAC 4 cut(s) 1006, 1255, 1778, 2417
MbiI CCGCTC 1 cut(s) 92
MfeI CAATTG 2 cut(s) 456, 2129
MflI RGATCY 5 cut(s) 34, 688, 1582, 1817, 2047
MhlI GDGCHC 4 cut(s) 123, 1043, 1814, 2200
MlyI GAGTC 5 cut(s) 120, 169, 852, 1870, 2060
MroXI GAANNNNTTC 3 cut(s) 369, 2151, 2402
MslI CAYNNNNRTG 1 cut(s) 333
MspCI CTTAAG 2 cut(s) 608, 1631
MspI CCGG 1 cut(s) 2284
MspR9I CCNGG 3 cut(s) 546, 1190, 2260
MunI CAATTG 2 cut(s) 456, 2129
Mva1269I GAATGC 1 cut(s) 1937
MvaI CCWGG 3 cut(s) 546, 1190, 2260
MvnI CGCG 1 cut(s) 141
MwoI GCNNNNNNNGC 8 cut(s) 67, 788, 832, 1026, 1238, 1713, 1943, 2306
NcoI CCATGG 1 cut(s) 1952
NlaIV GGNNCC 3 cut(s) 690, 1040, 1901
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 1255
NruI TCGCGA 1 cut(s) 141
NspI RCATGY 1 cut(s) 1855
PagI TCATGA 1 cut(s) 1066
PciI ACATGT 1 cut(s) 1851
PcsI WCGNNNNNNNCGW 1 cut(s) 637
PctI GAATGC 1 cut(s) 1937
PdmI GAANNNNTTC 3 cut(s) 369, 2151, 2402
PfeI GAWTC 7 cut(s) 86, 541, 585, 1063, 1142, 1672, 2134
Pfl23II CGTACG 1 cut(s) 2019
PfoI TCCNGGA 2 cut(s) 544, 1188
PkrI GCNGC 6 cut(s) 27, 222, 834, 1080, 1317, 1403
PleI GAGTC 5 cut(s) 120, 168, 852, 1869, 2060
PpsI GAGTC 5 cut(s) 120, 168, 852, 1869, 2060
Ppu21I YACGTR 1 cut(s) 2019
PpuMI RGGWCCY 1 cut(s) 1072
PscI ACATGT 1 cut(s) 1851
Psp124BI GAGCTC 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 1072
Psp6I CCWGG 3 cut(s) 544, 1188, 2258
PspEI GGTNACC 1 cut(s) 1778
PspFI CCCAGC 1 cut(s) 1654
PspGI CCWGG 3 cut(s) 544, 1188, 2258
PspLI CGTACG 1 cut(s) 2019
PspN4I GGNNCC 3 cut(s) 690, 1040, 1901
PspPI GGNCC 2 cut(s) 1072, 1900
PspPPI RGGWCCY 1 cut(s) 1072
PstI CTGCAG 1 cut(s) 1080
PsuI RGATCY 5 cut(s) 34, 688, 1582, 1817, 2047
RruI TCGCGA 1 cut(s) 141
RsaI GTAC 6 cut(s) 342, 1360, 1556, 1606, 2021, 2124
RsaNI GTAC 6 cut(s) 341, 1359, 1555, 1605, 2020, 2123
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 1 cut(s) 123
SatI GCNGC 6 cut(s) 26, 221, 833, 1079, 1316, 1402
Sau96I GGNCC 2 cut(s) 1072, 1900
ScaI AGTACT 1 cut(s) 342
SchI GAGTC 5 cut(s) 120, 169, 852, 1870, 2060
ScrFI CCNGG 3 cut(s) 546, 1190, 2260
SduI GDGCHC 4 cut(s) 123, 1043, 1814, 2200
SfaNI GCATC 7 cut(s) 30, 874, 1192, 1377, 1519, 1947, 2186
SfcI CTRYAG 2 cut(s) 928, 1076
SinI GGWCC 1 cut(s) 1072
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 5 cut(s) 439, 527, 608, 1631, 1821
SmoI CTYRAG 5 cut(s) 439, 527, 608, 1631, 1821
SsiI CCGC 7 cut(s) 26, 90, 223, 1402, 1705, 2070, 2300
SspI AATATT 2 cut(s) 765, 1686
SspMI CTAG 5 cut(s) 395, 840, 1319, 1587, 2156
SstI GAGCTC 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 544, 1188, 2258
StyI CCWWGG 1 cut(s) 1952
TaaI ACNGT 7 cut(s) 362, 503, 955, 1211, 1540, 1559, 2230
TaiI ACGT 1 cut(s) 2021
TaqI TCGA 2 cut(s) 494, 1092
TatI WGTACW 2 cut(s) 340, 2122
TauI GCSGC 2 cut(s) 28, 1404
TfiI GAWTC 7 cut(s) 86, 541, 585, 1063, 1142, 1672, 2134
TscAI CASTG 4 cut(s) 367, 1794, 1819, 2384
TseFI GTSAC 1 cut(s) 1255
TseI GCWGC 4 cut(s) 220, 832, 1078, 1315
Tsp45I GTSAC 1 cut(s) 1255
TspGWI ACGGA 1 cut(s) 187
TspRI CASTG 4 cut(s) 367, 1794, 1819, 2384
Vha464I CTTAAG 2 cut(s) 608, 1631
VneI GTGCAC 1 cut(s) 1810
VpaK11BI GGWCC 1 cut(s) 1072
XapI RAATTY 7 cut(s) 8, 352, 398, 997, 1989, 2147, 2387
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 1855
XcmI CCANNNNNNNNNTGG 1 cut(s) 1487
XmnI GAANNNNTTC 3 cut(s) 369, 2151, 2402
XspI CTAG 5 cut(s) 395, 840, 1319, 1587, 2156
ZrmI AGTACT 1 cut(s) 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.