Rroxscaffold_1G00019450

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0009092
Physical Location & Seq
Reverse (-)
23755901 .. 23766141
10241 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019450.1

Sequence Viewer

Length: 2265 bp
ATGTGCAGAGATGTCAAGAAAGCTGCGCTTTTTAAGGATCAAATCAGTAAAGCAGGTCTGCTTGGGTCTATACGTCCAGTTTCACAACCAGATATTGAGTTGGAGGAATTAGAGTTTCAACTTGGTGAGCACGAACATGAGCTAATTGAAATGAACTCTAATAGTGAAAGACTTCGACAATCATACAATGAGCTCCTGGAGTTCAAGATGGTGTTACAAAAGGCAAGTGGCTTTCTTGTTTCAAGTAATAGCCATGCTGTGACAGAGGAGACAGAACTAGAAGAGAATGTTTACTCAATGAATGACTATGGTGATTCAGTGTCATTGCTGGAGCAGGATATCAGACCGGGACCATCAGATCAATCTGGTTTAAATTTTGTTAGTGGGATTATTTGTAAATCCAAAGCTCTAAGGTTTGAGAGGATGCTGTTTCGTGCTACAAGGGGAAATATGCTTTTCAACCAAGCACCAGCAGATGAACATATCATGGATCCTTTATCTACTGAAATGGTTGAGCAGACAGTTTTTGTAGTCTTCTTCTCAGGGTTGCAGGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTATCCTGTTCCTGAAGATATAACTAAGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAGCAACTTTGGATGCAGGGATTCGCCACAGAAATAAGGCCCTTAATTCTGTAGGGTTTCACCTGGCAAAATGGATGAACATGGTAAGAAGGGAGAAAGCTGTATATGATACATTGAACATGTTAAACTTTGATGTTACCAAAAAATGCCTTGTTGGAGAAGGCTGGTGTCCTATATTTGCAAAAACAAAGATTCAGGAGGCATTGCAACGTGCAACATTTGATAGCAATTCACAAGTGGGTGTAATATTTCACGTGATGGATGCAATAGAATCACCTCCTACATATTTTAGAACCAACAACTTCACAAGTGCATTTCAGGAAATTGTTGATGCATATGGTGTAGCAAGATATCAAGAAGCAAATCCTGCAGTGTACACTGTTATTACATTTCCATTTCTCTTCGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTGTTGATGGGAGCATTAGTTCTTATAGCCCGTGAAAGAAAGCTCAGTGCTCAGAAACTCGGGAGCTTTATGGAGATGCTATTTGGTGGGCGCTATGTACTTCTGTTGATGTCTCTGTTCTCAATTTATTGTGGATTGATTTACAATGAGTTCTTCTCTGTCCCTTTTCACATATTTGGTGGGTCTGCTTACAAATGCAGAGATGCTACTTGCAGTGATGCACACACTGTTGGCTTAATCAAGTACCGGGATCCATACCCATTTGGTGTGGATCCTAGTTGGCGTGGAAGTCGTTCAGAACTTCCCTTTTTGAATTCTCTCAAAATGAAATTGTCTATTTTGTTGGGTGTGGCGCAAATGAACATAGGAATTATTCTCAGTTACTTCAATGCACGGTTTTTCAGCAGCTCAATTGATATAAGGTACCAGTTTGTGCCGCAGATGATCTTCCTTAACAGTCTTTTTGGATATCTTTCACTTCTAGTTGTCATCAAGTGGTGCACCGGCTCTAAGGCTGACCTTTATCATGTAATGATTTACATGTTCTTAAGTCCAACTGATGATCTTGGGGAAAATCAGTTGTTTTGGGGCCAAAGACCACTTCAGATCATTTTGTTACTCTTGGCTCTAATTGCAGTCCCCTGGATGCTCTTTCCAAAACCTTTCATTTTGAGGAAGCGTCATACAGAGAGGTTTCAAGGTCGCACATATGGGGTGCTTGGTACCTCTGAAATGGATCTTGACGTGGAAACTGATCCTGTGAGGCAACATCATGAGGAGTTTAATTTCAGTGAGGTGTTTGTGCACCAGATGATACACTCCATAGAGTTTGTTCTTGGTGCAGTTTCAAATACGGCGTCCTACCTTCGATTGTGGGCTTTGAGCTTGGCCCACTCAGAGTTGTCAACTGTGTTCTACGAGAAAGTCCTCCTCCTTGCCTGGGGGTATGACAGCTTTATCATCCGGTTAATTGGGTTAGCAGTTTTTGCCTTTGCAACTGCCTTCATATTGCTCATGATGGAGACACTCAGCGCTTTTCTTCATGCCTTGCGTCTTCATTGGGTGGAATTTCAAAATAAGTTCTACCTTGGTGATGGGTATAAGTTCAAACCTTTTTCCTTCGCCTCCATAGCTACTGAGGATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

754

Amino Acids

85.92

Weight (kDa)

5.93

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 25 - 745 2.3e-274 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
Acc65I GGTACC 2 cut(s) 1536, 1835
AccB1I GGYRCC 2 cut(s) 1536, 1835
AciI CCGC 1 cut(s) 1550
AclWI GGATC 9 cut(s) 45, 485, 498, 1358, 1371, 1379, 1392, 1857, 1862
AcsI RAATTY 4 cut(s) 373, 573, 1426, 2180
AcuI CTGAAG 2 cut(s) 633, 1700
AcvI CACGTG 1 cut(s) 928
AcyI GRCGYC 1 cut(s) 1970
AfaI GTAC 5 cut(s) 1049, 1212, 1358, 1538, 1837
AfeI AGCGCT 1 cut(s) 2146
AfiI CCNNNNNNNGG 1 cut(s) 2053
AflII CTTAAG 1 cut(s) 1660
AflIII ACRYGT 2 cut(s) 792, 1653
AjiI CACGTC 2 cut(s) 659, 1858
AjnI CCWGG 4 cut(s) 195, 735, 1754, 2051
AjuI GAANNNNNNNTTGG 2 cut(s) 1698, 1730
AloI GAACNNNNNNTCC 2 cut(s) 1116, 1148
Alw21I GWGCWC 5 cut(s) 132, 195, 1165, 1616, 1920
Alw26I GTCTC 3 cut(s) 263, 1230, 2129
Alw44I GTGCAC 2 cut(s) 1612, 1916
AlwI GGATC 9 cut(s) 45, 485, 498, 1358, 1371, 1379, 1392, 1857, 1862
Ama87I CYCGRG 1 cut(s) 1172
Aor51HI AGCGCT 1 cut(s) 2146
AoxI GGCC 4 cut(s) 582, 711, 1702, 2001
ApaLI GTGCAC 2 cut(s) 1612, 1916
ApeKI GCWGC 2 cut(s) 23, 1518
ApoI RAATTY 4 cut(s) 373, 573, 1426, 2180
ArsI GACNNNNNNTTYG 2 cut(s) 1955, 1987
Asp700I GAANNNNTTC 2 cut(s) 171, 1405
Asp718I GGTACC 2 cut(s) 1536, 1835
AspLEI GCGC 4 cut(s) 28, 1206, 1468, 2147
AspS9I GGNCC 4 cut(s) 350, 712, 1702, 2002
AsuC2I CCSGG 2 cut(s) 348, 1361
AsuHPI GGTGA 5 cut(s) 137, 323, 725, 939, 2216
AvaI CYCGRG 1 cut(s) 1172
AvaII GGWCC 1 cut(s) 350
BaeGI GKGCMC 2 cut(s) 1616, 1920
BamHI GGATCC 3 cut(s) 490, 1363, 1384
BanI GGYRCC 2 cut(s) 1536, 1835
BanII GRGCYC 1 cut(s) 195
BbrPI CACGTG 1 cut(s) 928
BbsI GAAGAC 2 cut(s) 526, 2159
Bbv12I GWGCWC 5 cut(s) 132, 195, 1165, 1616, 1920
BbvI GCAGC 2 cut(s) 10, 1530
BccI CCATC 6 cut(s) 202, 361, 925, 1114, 2125, 2201
BceAI ACGGC 1 cut(s) 1983
BciT130I CCWGG 4 cut(s) 197, 737, 1756, 2053
BcnI CCSGG 2 cut(s) 348, 1361
BcoDI GTCTC 3 cut(s) 263, 1230, 2129
BfaI CTAG 4 cut(s) 278, 642, 1389, 1595
BfmI CTRYAG 2 cut(s) 723, 1041
BfoI RGCGCY 2 cut(s) 1207, 2148
BfrI CTTAAG 1 cut(s) 1660
BfuAI ACCTGC 1 cut(s) 44
BisI GCNGC 3 cut(s) 24, 1519, 1550
BlsI GCNGC 3 cut(s) 25, 1520, 1551
Bme1390I CCNGG 6 cut(s) 197, 348, 737, 1361, 1756, 2053
Bme18I GGWCC 1 cut(s) 350
BmeT110I CYCGRG 1 cut(s) 1172
BmgBI CACGTC 2 cut(s) 659, 1858
BmgT120I GGNCC 4 cut(s) 350, 712, 1702, 2002
BmiI GGNNCC 7 cut(s) 351, 492, 1365, 1386, 1538, 1703, 1837
BmrFI CCNGG 6 cut(s) 197, 348, 737, 1361, 1756, 2053
BmrI ACTGGG 1 cut(s) 1105
BmsI GCATC 8 cut(s) 414, 676, 925, 994, 1179, 1306, 1321, 1749
BmuI ACTGGG 1 cut(s) 1105
BoxI GACNNNNGTC 1 cut(s) 1098
BpiI GAAGAC 2 cut(s) 526, 2159
BplI GAGNNNNNCTC 2 cut(s) 1253, 1285
BpmI CTGGAG 2 cut(s) 218, 350
BpuMI CCSGG 2 cut(s) 348, 1361
BsaAI YACGTR 1 cut(s) 928
BsaHI GRCGYC 1 cut(s) 1970
BsaJI CCNNGG 3 cut(s) 1754, 2052, 2200
BsaWI WCCGGW 1 cut(s) 2076
BsaXI ACNNNNNCTCC 2 cut(s) 1116, 1146
Bsc4I CCNNNNNNNGG 1 cut(s) 2053
Bse118I RCCGGY 1 cut(s) 1616
Bse1I ACTGG 3 cut(s) 77, 1100, 1540
Bse3DI GCAATG 2 cut(s) 323, 875
BseBI CCWGG 4 cut(s) 197, 737, 1756, 2053
BseDI CCNNGG 3 cut(s) 1754, 2052, 2200
BseGI GGATG 7 cut(s) 429, 691, 753, 940, 1764, 2073, 2260
BseLI CCNNNNNNNGG 1 cut(s) 2053
BseMI GCAATG 2 cut(s) 323, 875
BseMII CTCAG 7 cut(s) 555, 1171, 1178, 1504, 2022, 2155, 2241
BseNI ACTGG 3 cut(s) 77, 1100, 1540
BseRI GAGGAG 3 cut(s) 281, 1904, 2033
BseSI GKGCMC 2 cut(s) 1616, 1920
BseXI GCAGC 2 cut(s) 10, 1530
BsgI GTGCAG 2 cut(s) 25, 1974
BshFI GGCC 4 cut(s) 584, 713, 1704, 2003
BshNI GGYRCC 2 cut(s) 1536, 1835
BsiHKAI GWGCWC 5 cut(s) 132, 195, 1165, 1616, 1920
BsiHKCI CYCGRG 1 cut(s) 1172
BsiSI CCGG 4 cut(s) 347, 1360, 1617, 2077
BslFI GGGAC 4 cut(s) 363, 1106, 1259, 1736
BslI CCNNNNNNNGG 1 cut(s) 2053
BsmAI GTCTC 3 cut(s) 263, 1230, 2129
BsmFI GGGAC 4 cut(s) 363, 1106, 1259, 1736
BsnI GGCC 4 cut(s) 584, 713, 1704, 2003
BsoBI CYCGRG 1 cut(s) 1172
Bsp1286I GDGCHC 5 cut(s) 132, 195, 1165, 1616, 1920
Bsp1407I TGTACA 1 cut(s) 1047
BspACI CCGC 1 cut(s) 1550
BspANI GGCC 4 cut(s) 584, 713, 1704, 2003
BspCNI CTCAG 7 cut(s) 554, 1170, 1177, 1503, 2021, 2154, 2242
BspHI TCATGA 2 cut(s) 1885, 2127
BspLI GGNNCC 7 cut(s) 351, 492, 1365, 1386, 1538, 1703, 1837
BspMAI CTGCAG 1 cut(s) 1045
BspMI ACCTGC 1 cut(s) 44
BspPI GGATC 9 cut(s) 45, 485, 498, 1358, 1371, 1379, 1392, 1857, 1862
BspT107I GGYRCC 2 cut(s) 1536, 1835
BspTI CTTAAG 1 cut(s) 1660
BsrDI GCAATG 2 cut(s) 323, 875
BsrFI RCCGGY 1 cut(s) 1616
BsrGI TGTACA 1 cut(s) 1047
BsrI ACTGG 3 cut(s) 77, 1100, 1540
BssAI RCCGGY 1 cut(s) 1616
BssECI CCNNGG 3 cut(s) 1754, 2052, 2200
BssNI GRCGYC 1 cut(s) 1970
BssT1I CCWWGG 1 cut(s) 2200
Bst2UI CCWGG 4 cut(s) 197, 737, 1756, 2053
Bst4CI ACNGT 6 cut(s) 523, 1054, 1342, 1509, 1571, 2023
Bst6I CTCTTC 2 cut(s) 276, 1079
BstACI GRCGYC 1 cut(s) 1970
BstAFI CTTAAG 1 cut(s) 1660
BstAPI GCANNNNNTGC 2 cut(s) 1040, 2099
BstAUI TGTACA 1 cut(s) 1047
BstBAI YACGTR 1 cut(s) 928
BstC8I GCNNGC 1 cut(s) 552
BstF5I GGATG 7 cut(s) 429, 691, 753, 940, 1764, 2073, 2260
BstH2I RGCGCY 2 cut(s) 1207, 2148
BstHHI GCGC 4 cut(s) 28, 1206, 1468, 2147
BstMAI GTCTC 3 cut(s) 263, 1230, 2129
BstMWI GCNNNNNNNGC 5 cut(s) 590, 1040, 1745, 2099, 2243
BstNI CCWGG 4 cut(s) 197, 737, 1756, 2053
BstNSI RCATGY 2 cut(s) 796, 1657
BstPAI GACNNNNGTC 1 cut(s) 1098
BstSCI CCNGG 6 cut(s) 195, 346, 735, 1359, 1754, 2051
BstSFI CTRYAG 2 cut(s) 723, 1041
BstSLI GKGCMC 2 cut(s) 1616, 1920
BstV1I GCAGC 2 cut(s) 10, 1530
BstV2I GAAGAC 2 cut(s) 526, 2159
BstX2I RGATCY 4 cut(s) 490, 1363, 1384, 1849
BstYI RGATCY 4 cut(s) 490, 1363, 1384, 1849
BsuRI GGCC 4 cut(s) 584, 713, 1704, 2003
BtrI CACGTC 2 cut(s) 659, 1858
BtsCI GGATG 7 cut(s) 429, 691, 753, 940, 1764, 2073, 2260
BtsI GCAGTG 2 cut(s) 1050, 1333
BtsIMutI CAGTG 7 cut(s) 324, 1050, 1050, 1165, 1333, 1338, 1909
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 552
CciI TCATGA 2 cut(s) 1885, 2127
CfoI GCGC 4 cut(s) 28, 1206, 1468, 2147
Cfr10I RCCGGY 1 cut(s) 1616
Cfr13I GGNCC 4 cut(s) 350, 712, 1702, 2002
CseI GACGC 3 cut(s) 1781, 1959, 2153
Csp6I GTAC 5 cut(s) 1048, 1211, 1357, 1537, 1836
CviQI GTAC 5 cut(s) 1048, 1211, 1357, 1537, 1836
DraI TTTAAA 1 cut(s) 372
Eam1104I CTCTTC 2 cut(s) 276, 1079
EarI CTCTTC 2 cut(s) 276, 1079
Ecl136II GAGCTC 1 cut(s) 193
Eco130I CCWWGG 1 cut(s) 2200
Eco147I AGGCCT 1 cut(s) 584
Eco24I GRGCYC 1 cut(s) 195
Eco32I GATATC 3 cut(s) 340, 1025, 1583
Eco47I GGWCC 1 cut(s) 350
Eco47III AGCGCT 1 cut(s) 2146
Eco53kI GAGCTC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 633, 1700
Eco72I CACGTG 1 cut(s) 928
Eco88I CYCGRG 1 cut(s) 1172
EcoICRI GAGCTC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 712
EcoRI GAATTC 1 cut(s) 1426
EcoRII CCWGG 4 cut(s) 195, 735, 1754, 2051
EcoRV GATATC 3 cut(s) 340, 1025, 1583
EcoT14I CCWWGG 1 cut(s) 2200
EcoT22I ATGCAT 1 cut(s) 1009
EcoT38I GRGCYC 1 cut(s) 195
ErhI CCWWGG 1 cut(s) 2200
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 4 cut(s) 363, 1106, 1259, 1736
FauNDI CATATG 2 cut(s) 1009, 1822
Fnu4HI GCNGC 3 cut(s) 24, 1519, 1550
FokI GGATG 6 cut(s) 436, 698, 760, 947, 1771, 2060
FriOI GRGCYC 1 cut(s) 195
Fsp4HI GCNGC 3 cut(s) 24, 1519, 1550
FspBI CTAG 4 cut(s) 278, 642, 1389, 1595
GlaI GCGC 4 cut(s) 27, 1205, 1467, 2146
GluI GCNGC 3 cut(s) 24, 1519, 1550
GsuI CTGGAG 2 cut(s) 218, 350
HaeII RGCGCY 2 cut(s) 1207, 2148
HaeIII GGCC 4 cut(s) 584, 713, 1704, 2003
HapII CCGG 4 cut(s) 347, 1360, 1617, 2077
HgaI GACGC 3 cut(s) 1781, 1959, 2153
HhaI GCGC 4 cut(s) 28, 1206, 1468, 2147
Hin1I GRCGYC 1 cut(s) 1970
Hin6I GCGC 4 cut(s) 26, 1204, 1466, 2145
HinP1I GCGC 4 cut(s) 26, 1204, 1466, 2145
HincII GTYRAC 1 cut(s) 2019
HindII GTYRAC 1 cut(s) 2019
HinfI GANTC 5 cut(s) 314, 565, 694, 865, 944
HpaII CCGG 4 cut(s) 347, 1360, 1617, 2077
HphI GGTGA 5 cut(s) 137, 323, 725, 939, 2216
Hpy166II GTNNAC 6 cut(s) 292, 1048, 1050, 1614, 1918, 2019
Hpy188I TCNGA 8 cut(s) 344, 358, 570, 1167, 1411, 1719, 1843, 2011
Hpy8I GTNNAC 6 cut(s) 292, 1048, 1050, 1614, 1918, 2019
HpyAV CCTTC 5 cut(s) 756, 827, 1988, 2125, 2242
HpyCH4III ACNGT 6 cut(s) 523, 1054, 1342, 1509, 1571, 2023
HpyCH4IV ACGT 5 cut(s) 73, 658, 883, 927, 1857
HpyF10VI GCNNNNNNNGC 5 cut(s) 590, 1040, 1745, 2099, 2243
HpySE526I ACGT 5 cut(s) 73, 658, 883, 927, 1857
Hsp92I GRCGYC 1 cut(s) 1970
HspAI GCGC 4 cut(s) 26, 1204, 1466, 2145
KpnI GGTACC 2 cut(s) 1540, 1839
LmnI GCTCC 4 cut(s) 198, 331, 1124, 1176
Lsp1109I GCAGC 2 cut(s) 10, 1530
LweI GCATC 8 cut(s) 414, 676, 925, 994, 1179, 1306, 1321, 1749
MaeI CTAG 4 cut(s) 278, 642, 1389, 1595
MaeII ACGT 5 cut(s) 73, 658, 883, 927, 1857
MaeIII GTNAC 5 cut(s) 213, 259, 808, 1493, 1728
MboII GAAGA 9 cut(s) 293, 526, 529, 626, 1066, 1258, 1552, 2144, 2159
MfeI CAATTG 1 cut(s) 1524
MflI RGATCY 4 cut(s) 490, 1363, 1384, 1849
MhlI GDGCHC 5 cut(s) 132, 195, 1165, 1616, 1920
MmeI TCCRAC 3 cut(s) 81, 808, 1691
Mph1103I ATGCAT 1 cut(s) 1009
MroXI GAANNNNTTC 2 cut(s) 171, 1405
MseI TTAA 9 cut(s) 33, 371, 717, 797, 1349, 1566, 1661, 1896, 2081
MslI CAYNNNNRTG 1 cut(s) 135
MspCI CTTAAG 1 cut(s) 1660
MspI CCGG 4 cut(s) 347, 1360, 1617, 2077
MspR9I CCNGG 6 cut(s) 197, 348, 737, 1361, 1756, 2053
MunI CAATTG 1 cut(s) 1524
MvaI CCWGG 4 cut(s) 197, 737, 1756, 2053
MwoI GCNNNNNNNGC 5 cut(s) 590, 1040, 1745, 2099, 2243
NciI CCSGG 2 cut(s) 348, 1361
NdeI CATATG 2 cut(s) 1009, 1822
NlaIV GGNNCC 7 cut(s) 351, 492, 1365, 1386, 1538, 1703, 1837
NmuCI GTSAC 1 cut(s) 259
NsiI ATGCAT 1 cut(s) 1009
NspI RCATGY 2 cut(s) 796, 1657
PagI TCATGA 2 cut(s) 1885, 2127
PceI AGGCCT 1 cut(s) 584
PciI ACATGT 2 cut(s) 792, 1653
PdmI GAANNNNTTC 2 cut(s) 171, 1405
PfeI GAWTC 5 cut(s) 314, 565, 694, 865, 944
PfoI TCCNGGA 1 cut(s) 195
PkrI GCNGC 3 cut(s) 25, 1520, 1551
PmaCI CACGTG 1 cut(s) 928
PmlI CACGTG 1 cut(s) 928
Ppu21I YACGTR 1 cut(s) 928
PscI ACATGT 2 cut(s) 792, 1653
PshAI GACNNNNGTC 1 cut(s) 1098
Psp124BI GAGCTC 1 cut(s) 195
Psp6I CCWGG 4 cut(s) 195, 735, 1754, 2051
PspCI CACGTG 1 cut(s) 928
PspGI CCWGG 4 cut(s) 195, 735, 1754, 2051
PspN4I GGNNCC 7 cut(s) 351, 492, 1365, 1386, 1538, 1703, 1837
PspPI GGNCC 4 cut(s) 350, 712, 1702, 2002
PstI CTGCAG 1 cut(s) 1045
PsuI RGATCY 4 cut(s) 490, 1363, 1384, 1849
RsaI GTAC 5 cut(s) 1049, 1212, 1358, 1538, 1837
RsaNI GTAC 5 cut(s) 1048, 1211, 1357, 1537, 1836
RseI CAYNNNNRTG 1 cut(s) 135
SacI GAGCTC 1 cut(s) 195
SaqAI TTAA 9 cut(s) 33, 371, 717, 797, 1349, 1566, 1661, 1896, 2081
SatI GCNGC 3 cut(s) 24, 1519, 1550
Sau96I GGNCC 4 cut(s) 350, 712, 1702, 2002
ScrFI CCNGG 6 cut(s) 197, 348, 737, 1361, 1756, 2053
SduI GDGCHC 5 cut(s) 132, 195, 1165, 1616, 1920
SfaNI GCATC 8 cut(s) 414, 676, 925, 994, 1179, 1306, 1321, 1749
SfcI CTRYAG 2 cut(s) 723, 1041
SinI GGWCC 1 cut(s) 350
SmiMI CAYNNNNRTG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 1660
SmoI CTYRAG 1 cut(s) 1660
SseBI AGGCCT 1 cut(s) 584
SsiI CCGC 1 cut(s) 1550
SspI AATATT 1 cut(s) 921
SspMI CTAG 4 cut(s) 278, 642, 1389, 1595
SstI GAGCTC 1 cut(s) 195
StuI AGGCCT 1 cut(s) 584
StyD4I CCNGG 6 cut(s) 195, 346, 735, 1359, 1754, 2051
StyI CCWWGG 1 cut(s) 2200
TaaI ACNGT 6 cut(s) 523, 1054, 1342, 1509, 1571, 2023
TaiI ACGT 5 cut(s) 76, 661, 886, 930, 1860
TaqI TCGA 2 cut(s) 175, 1981
TatI WGTACW 2 cut(s) 1047, 1210
TauI GCSGC 1 cut(s) 1552
TfiI GAWTC 5 cut(s) 314, 565, 694, 865, 944
Tru1I TTAA 9 cut(s) 33, 371, 717, 797, 1349, 1566, 1661, 1896, 2081
Tru9I TTAA 9 cut(s) 33, 371, 717, 797, 1349, 1566, 1661, 1896, 2081
TscAI CASTG 7 cut(s) 324, 1050, 1057, 1165, 1333, 1345, 1909
TseFI GTSAC 1 cut(s) 259
TseI GCWGC 2 cut(s) 23, 1518
Tsp45I GTSAC 1 cut(s) 259
TspRI CASTG 7 cut(s) 324, 1050, 1057, 1165, 1333, 1345, 1909
Vha464I CTTAAG 1 cut(s) 1660
VneI GTGCAC 2 cut(s) 1612, 1916
VpaK11BI GGWCC 1 cut(s) 350
XapI RAATTY 4 cut(s) 373, 573, 1426, 2180
XceI RCATGY 2 cut(s) 796, 1657
XmnI GAANNNNTTC 2 cut(s) 171, 1405
XspI CTAG 4 cut(s) 278, 642, 1389, 1595
Zsp2I ATGCAT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.