Rw5G037720

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0009092
Physical Location & Seq
Forward (+)
66382346 .. 66391614
9269 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037720.1

Sequence Viewer

Length: 2463 bp
ATGGAGAAGTTCCTGGACAAGTTGCCCTCCATGGATCTGATGCGCTCCGAGAAGATGACCTTCGTCCAGCTCATCATCCCCGTCGAGTCTGCTCACCGCACCATTTCCTATCTCGGCGAAATCGGCCTCCTCCAATTCCGTGATTTAAATGCTGATAAAAGCCCTTTCCAGCTAACCTTTGTTAATCAGGTGAAGCGATGTGCAGAGATGTCAAGAAAGCTGCGCTTTTTTAAGGATCAAATCAGTAAAGCTGGTCTGCTTGGGTCTATACGTCCAGTTTCACAACCAGATATCGAGTTGGAGGAATTAGAGTTTCAACTTGGTGAGCACGAACATGAGCTAATTGAAATGAACTCTAATAGTGAAAGACTTCGACAATCATACAATGAGCTCCTGGAGTTCAAGATGGTATTACTAAAGGCAAGTGGCTTTCTTGTTTCAAGTAATAGCCATGCTGTCGCAGAGGAAACAGAACTAGAAGAGAATGTTTACTCAATGAATGATTATGGTGATTCAGTGTCATTGCTGGAGCAGGATATCAGACGGGGATCATCAGATCAATCTGGTTTGAATTTTGTTAGTGGGATTATTTGTAAATCTAAAGCTCTAAGGTTTGAGAGGATGCTGTTTCGTGCTACAAGGGGAAATATGCTTTTCAACCAAGCACCAGCAGATGAACATATCATGGATCCTTTATCTACTGAAATGGTTGAGCAGACAGTTTTTGTAGTCTTCTTCTCAGGGTTGCAAGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTACCCTGTTCCTGAAGATATAACTAAGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAGCAACTTTGGATGCAGGGATTCGCCACAGAAATAAGGCCCTTACTTCTGTAGGGTTTCACTTGGCAAAATGGATGAACATGGTAAGAAGGGAGAAAGCTGTATATGATACATTGAACATGTTAAACTTTGATGTTACCAAAAAATGCCTTGTTGGAGAAGGCTGGTGTCCTATATTTGCAAAAACAAAGATTCAGGAGGCATTGGAACGTGCAACATTTGATAGCAATTCACAAGTGGGTGTAATATTTCATGTGATGGATGCAATAGAATCACCTCCTACATATTTTAGAACCAACAACTTCACAAGTGCATTTCAGGAAATTGTTGATGCATATGGCGTAGCAAGATATCAAGAAGCAAATCCTGCAGTGTACACTGTTATTACATTTCCATTTCTTTTCGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTGTTGATGGGAGCATTAGTTCTTATAGCCCGTGAAAGAAAGCTCAGTGCTCAGAAACTCGGGAGCTTTATGGAGATGCTATTTGGTGGGCGTTATGTACTTCTTTTAATGTCTCTGTTCTCAATTTACTGTGGGTTGATTTACAATGAGTTCTTCTCTGTCCCTTTTCACATATTTGGTGGGTCTGCTTACAAATGCAGAGATGCTTCTTGCAGTGATGCACACACTGTTGGCTTAATCAAGTACAGAGATCCATACCCATTTGGTGTGGATCCTAGTTGGCGTGGAAGTCGTTCAGAACTGCCCTTTTTGAATTCTCTCAAAATGAAATTGTCTATTTTGTTGGGTGTGGCGCAAATGAATGTAGGAATTATTCTCAGTTACTTCAATGCACGGTTTTTCAGCAGCTCAATTGATATAAGGTACCAGTTTGTGCCGCAGATGATCTTCCTTAACAGTCTTTTTGGATATCTTTCACTTCTAGTTGTCATCAAGTGGTGCACCGGCTCTAAGGCTGACCTCTATCATGTAATGATTTACATGTTCTTAAGTCCAACTGATGAACTTGGGGAAAATCAGTTGTTTTGGGGCCAAAGACCACTTCAGATCATTTTGTTACTCTTGGCTCTAATTGCAGTCCCCTGGATGCTCTTTCCAAAACCTTTCATTTTGAGGAAGCGTCATACAGAGAGGTTTCAAGGTCGCACATATGGGATGCTTGGTACCTCTGAAATGGATCTTGACGTGGAAACTGATCCTGTGAGGCAACATCATGAGGAGTTTAATTTCAGTGAGGTGTTTGTGCACCAGATGATACACTCCATAGAGTTTGTTCTTGGTGCAGTTTCAAATACGGCGTCCTACCTTCGATTGTGGGCTTTGAGCTTGGCCCACTCAGAGTTGTCAACTGTGTTCTACGAGAAAGTCCTTCTCCTCGCCTGGGGGTATGACAGCTTTATCGTCCGGTTAATTGGGTTAGCAGTTTTTGCCTTTGCAACTGCCTTCATATTGCTCATGATGGAGACACTCAGCGCTTTTCTTCATGCCTTGCGTCTTCATTGGGTGGAATTTCAAAATAAGTTCTACCTTGGTGATGGGTATAAGTTCAAACCTTTTTCCTTCGCCTCCATAGCTACTGAGGATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

820

Amino Acids

93.72

Weight (kDa)

6.04

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 37 - 811 8.4e-292 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1734, 2033
AccB1I GGYRCC 2 cut(s) 1734, 2033
AciI CCGC 2 cut(s) 97, 1748
AcsI RAATTY 4 cut(s) 571, 771, 1624, 2378
AcuI CTGAAG 2 cut(s) 831, 1898
AcyI GRCGYC 1 cut(s) 2168
AfaI GTAC 5 cut(s) 1247, 1410, 1556, 1736, 2035
AfeI AGCGCT 1 cut(s) 2344
AfiI CCNNNNNNNGG 1 cut(s) 2251
AflII CTTAAG 1 cut(s) 1858
AflIII ACRYGT 2 cut(s) 990, 1851
AjiI CACGTC 2 cut(s) 857, 2056
AjnI CCWGG 4 cut(s) 12, 393, 1952, 2249
AjuI GAANNNNNNNTTGG 2 cut(s) 1896, 1928
AloI GAACNNNNNNTCC 2 cut(s) 1314, 1346
Alw21I GWGCWC 5 cut(s) 330, 393, 1363, 1814, 2118
Alw26I GTCTC 2 cut(s) 1428, 2327
Alw44I GTGCAC 2 cut(s) 1810, 2114
Ama87I CYCGRG 1 cut(s) 1370
Aor51HI AGCGCT 1 cut(s) 2344
AoxI GGCC 5 cut(s) 124, 780, 909, 1900, 2199
ApaLI GTGCAC 2 cut(s) 1810, 2114
ApeKI GCWGC 2 cut(s) 220, 1716
ApoI RAATTY 4 cut(s) 571, 771, 1624, 2378
ArsI GACNNNNNNTTYG 2 cut(s) 2153, 2185
Asp700I GAANNNNTTC 2 cut(s) 369, 1603
Asp718I GGTACC 2 cut(s) 1734, 2033
AspLEI GCGC 4 cut(s) 45, 225, 1666, 2345
AspS9I GGNCC 3 cut(s) 910, 1900, 2200
AsuHPI GGTGA 6 cut(s) 86, 202, 335, 521, 1137, 2414
AvaI CYCGRG 1 cut(s) 1370
BaeGI GKGCMC 2 cut(s) 1814, 2118
BamHI GGATCC 2 cut(s) 688, 1582
BanI GGYRCC 2 cut(s) 1734, 2033
BanII GRGCYC 1 cut(s) 393
BbsI GAAGAC 2 cut(s) 724, 2357
Bbv12I GWGCWC 5 cut(s) 330, 393, 1363, 1814, 2118
BbvI GCAGC 2 cut(s) 207, 1728
BccI CCATC 5 cut(s) 400, 1123, 1312, 2323, 2399
BceAI ACGGC 1 cut(s) 2181
BciT130I CCWGG 4 cut(s) 14, 395, 1954, 2251
BcoDI GTCTC 2 cut(s) 1428, 2327
BfaI CTAG 4 cut(s) 476, 840, 1587, 1793
BfmI CTRYAG 2 cut(s) 921, 1239
BfoI RGCGCY 1 cut(s) 2346
BfrI CTTAAG 1 cut(s) 1858
BisI GCNGC 3 cut(s) 221, 1717, 1748
BlsI GCNGC 3 cut(s) 222, 1718, 1749
Bme1390I CCNGG 4 cut(s) 14, 395, 1954, 2251
BmeT110I CYCGRG 1 cut(s) 1370
BmgBI CACGTC 2 cut(s) 857, 2056
BmgT120I GGNCC 3 cut(s) 910, 1900, 2200
BmiI GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2035
BmrFI CCNGG 4 cut(s) 14, 395, 1954, 2251
BmrI ACTGGG 1 cut(s) 1303
BmuI ACTGGG 1 cut(s) 1303
BoxI GACNNNNGTC 2 cut(s) 62, 1296
BpiI GAAGAC 2 cut(s) 724, 2357
BplI GAGNNNNNCTC 2 cut(s) 1451, 1483
BpmI CTGGAG 2 cut(s) 416, 548
BsaHI GRCGYC 1 cut(s) 2168
BsaJI CCNNGG 4 cut(s) 30, 1952, 2250, 2398
BsaWI WCCGGW 1 cut(s) 2274
BsaXI ACNNNNNCTCC 2 cut(s) 1314, 1344
Bsc4I CCNNNNNNNGG 1 cut(s) 2251
Bse118I RCCGGY 1 cut(s) 1814
Bse1I ACTGG 3 cut(s) 275, 1298, 1738
Bse3DI GCAATG 1 cut(s) 521
BseBI CCWGG 4 cut(s) 14, 395, 1954, 2251
BseDI CCNNGG 4 cut(s) 30, 1952, 2250, 2398
BseGI GGATG 8 cut(s) 75, 627, 889, 951, 1138, 1962, 2031, 2458
BseLI CCNNNNNNNGG 1 cut(s) 2251
BseMI GCAATG 1 cut(s) 521
BseMII CTCAG 7 cut(s) 753, 1369, 1376, 1702, 2220, 2353, 2439
BseNI ACTGG 3 cut(s) 275, 1298, 1738
BseRI GAGGAG 3 cut(s) 119, 2102, 2234
BseSI GKGCMC 2 cut(s) 1814, 2118
BseXI GCAGC 2 cut(s) 207, 1728
BsgI GTGCAG 2 cut(s) 222, 2172
BshFI GGCC 5 cut(s) 126, 782, 911, 1902, 2201
BshNI GGYRCC 2 cut(s) 1734, 2033
BsiHKAI GWGCWC 5 cut(s) 330, 393, 1363, 1814, 2118
BsiHKCI CYCGRG 1 cut(s) 1370
BsiSI CCGG 2 cut(s) 1815, 2275
BslFI GGGAC 3 cut(s) 1304, 1457, 1934
BslI CCNNNNNNNGG 1 cut(s) 2251
BsmAI GTCTC 2 cut(s) 1428, 2327
BsmFI GGGAC 3 cut(s) 1304, 1457, 1934
BsnI GGCC 5 cut(s) 126, 782, 911, 1902, 2201
BsoBI CYCGRG 1 cut(s) 1370
Bsp1286I GDGCHC 5 cut(s) 330, 393, 1363, 1814, 2118
Bsp1407I TGTACA 1 cut(s) 1245
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 2 cut(s) 97, 1748
BspANI GGCC 5 cut(s) 126, 782, 911, 1902, 2201
BspCNI CTCAG 7 cut(s) 752, 1368, 1375, 1701, 2219, 2352, 2440
BspHI TCATGA 2 cut(s) 2083, 2325
BspLI GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2035
BspMAI CTGCAG 1 cut(s) 1243
BspT107I GGYRCC 2 cut(s) 1734, 2033
BspTI CTTAAG 1 cut(s) 1858
BsrDI GCAATG 1 cut(s) 521
BsrFI RCCGGY 1 cut(s) 1814
BsrGI TGTACA 1 cut(s) 1245
BsrI ACTGG 3 cut(s) 275, 1298, 1738
BssAI RCCGGY 1 cut(s) 1814
BssECI CCNNGG 4 cut(s) 30, 1952, 2250, 2398
BssNI GRCGYC 1 cut(s) 2168
BssT1I CCWWGG 2 cut(s) 30, 2398
Bst2UI CCWGG 4 cut(s) 14, 395, 1954, 2251
Bst4CI ACNGT 7 cut(s) 721, 1252, 1442, 1540, 1707, 1769, 2221
Bst6I CTCTTC 1 cut(s) 474
BstACI GRCGYC 1 cut(s) 2168
BstAFI CTTAAG 1 cut(s) 1858
BstAPI GCANNNNNTGC 2 cut(s) 1238, 2297
BstAUI TGTACA 1 cut(s) 1245
BstC8I GCNNGC 1 cut(s) 750
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 8 cut(s) 75, 627, 889, 951, 1138, 1962, 2031, 2458
BstH2I RGCGCY 1 cut(s) 2346
BstHHI GCGC 4 cut(s) 45, 225, 1666, 2345
BstMAI GTCTC 2 cut(s) 1428, 2327
BstMWI GCNNNNNNNGC 6 cut(s) 123, 788, 1238, 1943, 2297, 2441
BstNI CCWGG 4 cut(s) 14, 395, 1954, 2251
BstNSI RCATGY 2 cut(s) 994, 1855
BstPAI GACNNNNGTC 2 cut(s) 62, 1296
BstSCI CCNGG 4 cut(s) 12, 393, 1952, 2249
BstSFI CTRYAG 2 cut(s) 921, 1239
BstSLI GKGCMC 2 cut(s) 1814, 2118
BstV1I GCAGC 2 cut(s) 207, 1728
BstV2I GAAGAC 2 cut(s) 724, 2357
BstX2I RGATCY 5 cut(s) 34, 688, 1561, 1582, 2047
BstYI RGATCY 5 cut(s) 34, 688, 1561, 1582, 2047
BsuRI GGCC 5 cut(s) 126, 782, 911, 1902, 2201
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 2 cut(s) 857, 2056
BtsCI GGATG 8 cut(s) 75, 627, 889, 951, 1138, 1962, 2031, 2458
BtsI GCAGTG 2 cut(s) 1248, 1531
BtsIMutI CAGTG 7 cut(s) 522, 1248, 1248, 1363, 1531, 1536, 2107
Cac8I GCNNGC 1 cut(s) 750
CciI TCATGA 2 cut(s) 2083, 2325
CfoI GCGC 4 cut(s) 45, 225, 1666, 2345
Cfr10I RCCGGY 1 cut(s) 1814
Cfr13I GGNCC 3 cut(s) 910, 1900, 2200
CseI GACGC 3 cut(s) 1979, 2157, 2351
Csp6I GTAC 5 cut(s) 1246, 1409, 1555, 1735, 2034
CviQI GTAC 5 cut(s) 1246, 1409, 1555, 1735, 2034
DraI TTTAAA 1 cut(s) 147
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Ecl136II GAGCTC 1 cut(s) 391
Eco130I CCWWGG 2 cut(s) 30, 2398
Eco147I AGGCCT 1 cut(s) 782
Eco24I GRGCYC 1 cut(s) 393
Eco32I GATATC 4 cut(s) 292, 538, 1223, 1781
Eco47III AGCGCT 1 cut(s) 2344
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 2 cut(s) 831, 1898
Eco88I CYCGRG 1 cut(s) 1370
EcoICRI GAGCTC 1 cut(s) 391
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 1 cut(s) 1624
EcoRII CCWGG 4 cut(s) 12, 393, 1952, 2249
EcoRV GATATC 4 cut(s) 292, 538, 1223, 1781
EcoT14I CCWWGG 2 cut(s) 30, 2398
EcoT22I ATGCAT 1 cut(s) 1207
EcoT38I GRGCYC 1 cut(s) 393
ErhI CCWWGG 2 cut(s) 30, 2398
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 209, 241
FaqI GGGAC 3 cut(s) 1304, 1457, 1934
FauNDI CATATG 2 cut(s) 1207, 2020
Fnu4HI GCNGC 3 cut(s) 221, 1717, 1748
FokI GGATG 7 cut(s) 62, 634, 896, 958, 1145, 1969, 2038
FriOI GRGCYC 1 cut(s) 393
Fsp4HI GCNGC 3 cut(s) 221, 1717, 1748
FspBI CTAG 4 cut(s) 476, 840, 1587, 1793
GlaI GCGC 4 cut(s) 44, 224, 1665, 2344
GluI GCNGC 3 cut(s) 221, 1717, 1748
GsuI CTGGAG 2 cut(s) 416, 548
HaeII RGCGCY 1 cut(s) 2346
HaeIII GGCC 5 cut(s) 126, 782, 911, 1902, 2201
HapII CCGG 2 cut(s) 1815, 2275
HgaI GACGC 3 cut(s) 1979, 2157, 2351
HhaI GCGC 4 cut(s) 45, 225, 1666, 2345
Hin1I GRCGYC 1 cut(s) 2168
Hin6I GCGC 4 cut(s) 43, 223, 1664, 2343
HinP1I GCGC 4 cut(s) 43, 223, 1664, 2343
HincII GTYRAC 1 cut(s) 2217
HindII GTYRAC 1 cut(s) 2217
HinfI GANTC 6 cut(s) 86, 512, 763, 892, 1063, 1142
HpaII CCGG 2 cut(s) 1815, 2275
HphI GGTGA 6 cut(s) 86, 202, 335, 521, 1137, 2414
Hpy166II GTNNAC 6 cut(s) 490, 1246, 1248, 1812, 2116, 2217
Hpy8I GTNNAC 6 cut(s) 490, 1246, 1248, 1812, 2116, 2217
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 7 cut(s) 70, 954, 1025, 2186, 2249, 2323, 2440
HpyCH4III ACNGT 7 cut(s) 721, 1252, 1442, 1540, 1707, 1769, 2221
HpyCH4IV ACGT 4 cut(s) 271, 856, 1081, 2055
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 788, 1238, 1943, 2297, 2441
HpySE526I ACGT 4 cut(s) 271, 856, 1081, 2055
Hsp92I GRCGYC 1 cut(s) 2168
HspAI GCGC 4 cut(s) 43, 223, 1664, 2343
KpnI GGTACC 2 cut(s) 1738, 2037
LmnI GCTCC 5 cut(s) 50, 396, 529, 1322, 1374
Lsp1109I GCAGC 2 cut(s) 207, 1728
MaeI CTAG 4 cut(s) 476, 840, 1587, 1793
MaeII ACGT 4 cut(s) 271, 856, 1081, 2055
MaeIII GTNAC 3 cut(s) 1006, 1691, 1926
MboII GAAGA 9 cut(s) 64, 491, 724, 727, 824, 1456, 1750, 2342, 2357
MfeI CAATTG 1 cut(s) 1722
MflI RGATCY 5 cut(s) 34, 688, 1561, 1582, 2047
MhlI GDGCHC 5 cut(s) 330, 393, 1363, 1814, 2118
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 279, 1006, 1889
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 2 cut(s) 369, 1603
MslI CAYNNNNRTG 1 cut(s) 333
MspCI CTTAAG 1 cut(s) 1858
MspI CCGG 2 cut(s) 1815, 2275
MspR9I CCNGG 4 cut(s) 14, 395, 1954, 2251
MunI CAATTG 1 cut(s) 1722
MvaI CCWGG 4 cut(s) 14, 395, 1954, 2251
MwoI GCNNNNNNNGC 6 cut(s) 123, 788, 1238, 1943, 2297, 2441
NcoI CCATGG 1 cut(s) 30
NdeI CATATG 2 cut(s) 1207, 2020
NlaIV GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2035
NmeAIII GCCGAG 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 2 cut(s) 994, 1855
PagI TCATGA 2 cut(s) 2083, 2325
PceI AGGCCT 1 cut(s) 782
PciI ACATGT 2 cut(s) 990, 1851
PdmI GAANNNNTTC 2 cut(s) 369, 1603
PfeI GAWTC 5 cut(s) 512, 763, 892, 1063, 1142
PfoI TCCNGGA 2 cut(s) 12, 393
PkrI GCNGC 3 cut(s) 222, 1718, 1749
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PscI ACATGT 2 cut(s) 990, 1851
PshAI GACNNNNGTC 2 cut(s) 62, 1296
Psp124BI GAGCTC 1 cut(s) 393
Psp6I CCWGG 4 cut(s) 12, 393, 1952, 2249
PspGI CCWGG 4 cut(s) 12, 393, 1952, 2249
PspN4I GGNNCC 5 cut(s) 690, 1584, 1736, 1901, 2035
PspPI GGNCC 3 cut(s) 910, 1900, 2200
PstI CTGCAG 1 cut(s) 1243
PsuI RGATCY 5 cut(s) 34, 688, 1561, 1582, 2047
RsaI GTAC 5 cut(s) 1247, 1410, 1556, 1736, 2035
RsaNI GTAC 5 cut(s) 1246, 1409, 1555, 1735, 2034
RseI CAYNNNNRTG 1 cut(s) 333
SacI GAGCTC 1 cut(s) 393
SatI GCNGC 3 cut(s) 221, 1717, 1748
Sau96I GGNCC 3 cut(s) 910, 1900, 2200
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 4 cut(s) 14, 395, 1954, 2251
SduI GDGCHC 5 cut(s) 330, 393, 1363, 1814, 2118
SfcI CTRYAG 2 cut(s) 921, 1239
SmiI ATTTAAAT 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 333
SmlI CTYRAG 1 cut(s) 1858
SmoI CTYRAG 1 cut(s) 1858
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 2 cut(s) 97, 1748
SspI AATATT 1 cut(s) 1119
SspMI CTAG 4 cut(s) 476, 840, 1587, 1793
SstI GAGCTC 1 cut(s) 393
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 4 cut(s) 12, 393, 1952, 2249
StyI CCWWGG 2 cut(s) 30, 2398
SwaI ATTTAAAT 1 cut(s) 147
TaaI ACNGT 7 cut(s) 721, 1252, 1442, 1540, 1707, 1769, 2221
TaiI ACGT 4 cut(s) 274, 859, 1084, 2058
TaqI TCGA 4 cut(s) 84, 294, 373, 2179
TatI WGTACW 3 cut(s) 1245, 1408, 1554
TauI GCSGC 1 cut(s) 1750
TfiI GAWTC 5 cut(s) 512, 763, 892, 1063, 1142
TscAI CASTG 7 cut(s) 522, 1248, 1255, 1363, 1531, 1543, 2107
TseI GCWGC 2 cut(s) 220, 1716
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 7 cut(s) 522, 1248, 1255, 1363, 1531, 1543, 2107
Vha464I CTTAAG 1 cut(s) 1858
VneI GTGCAC 2 cut(s) 1810, 2114
XapI RAATTY 4 cut(s) 571, 771, 1624, 2378
XceI RCATGY 2 cut(s) 994, 1855
XmnI GAANNNNTTC 2 cut(s) 369, 1603
XspI CTAG 4 cut(s) 476, 840, 1587, 1793
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.