Rh5DG427500

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0009092
Physical Location & Seq
Forward (+)
68149948 .. 68159826
9879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG427500.1

Sequence Viewer

Length: 2115 bp
ATGAACTCTAATAGTGAAAGACTTCGACAATCATACAATGAGCTCCTGGAGTTCAAGATGGTATTACAAAAGGCAAGTGGCTTTCTTGTTTCAAGTAATAGCCATGCTGTCACAGAGGAAACAGAACTAGAAGAGAATGTTTACTCAATGAATGACTATGGAGATTCAGTGTCATTGCTGGAGCAGGATATCAGACGGGGACCATCAGATCAATCTGGTTTGAATTTTGTTAGTGGGATTATTTGTAAATCTAAAGCTCTAAGGTTTGAGAGGATGCTGTTTCGTGCTACAAGGGGAAATATGCTTTTCAACCAAGCACCAGCAGATGAACATATCATGGATCCTTTATCTACTGAAATGGTTGAGCAGACAGTTTTTGTAGTCTTCTTCTCAGGGTTGCAGGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTACCCTGTTCCTGAAGATATAACTAAGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAGCAACTTTGGATGCAGGGATTCGCCACAGAAATAAGGCCCTTACTTCTGTAGGGTTTCACCTGGCAAAATGGATGAACATGGTAAGAAGGGAGAAAGCTGTATATGATACATTGAACATGTTAAACTTTGATGTTACCAAAAAATGCCTTGTTGGAGAAGGCTGGTGTCCTATATTTGCAAAAACAAAGATTCAGGAGGCATTGCAACGTGCAACATTTGATAGCAATTCACAAGTGGGTGTAATATTTCATGTGATGGATGCAATAGAATCACCTCCTACATATTTTAGAACCAACAACTTCACAAGTGCATTTCAGGAAATTGTTGATGCATATGGCGTAGCAAGATATCAAGAAGCAAATCCTGCAGTGTACACTGTCATTACATTTCCATTTCTTTTCGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTGTTGATGGGAGCATTAGTTCTTATAGCCCGTGAAAGAAAGCTCAGTGCTCAGAAACTCGGGAGCTTTATGGAGATGCTATTTGGTGGGCGCTATGTACTTCTTTTAATGTCTCTGTTCTCAATTTACTGTGGGTTGATTTACAATGAGTTCTTCTCTGTCCCTTTTCACATATTTGGTGGGTCTGCTTACAAATGCAGAGATGCTTCTTGCAGTGATGCACACACTGTTGGCTTAATCAAGTACAGAGATCCATACCCATTTGGTGTGGATCCTAGTTGGCGTGGAAGTCGTTCAGAACTGCCCTTTTTGAATTCTCTCAAAATGAAATTGTCTATTTTGTTGGGTGTGGCACAAATGAACGTAGGAATTATTCTCAGTTACTTCAATGCACGGTTTTTCAGCAGCTCAATTGATGTAAGGTACCAGTTTGTGCCGCAGATGATCTTCCTTAACAGTCTTTTTGGATATCTTTCACTTCTAGTTGTCATCAAGTGGTGCACCGGCTCTAAGGCTGACCTCTATCATGTGATGATTTACATGTTCTTAAGTCCAACTGATGATCTTGGGGAAAATCAGTTGTTTTGGGGCCAAAGACCACTTCAGATCATTTTGTTACTCTTGGCTCTAATTGCAGTCCCTTGGATGCTCTTTCCAAAACCTTTCATTTTGAGGAAGCGTCATACAGAGAGGTTTCAAGGTCGCACATATGGGATGCTTGGTACCTCTGAAATGGATCTTGACGTGGAAACTGATCCTGTGAGGCAACATCATGAGGAGTTTAATTTCAGTGAGGTGTTTGTGCACCAGATGATACACTCCATAGAGTTTGTTCTTGGTGCAGTTTCAAATACGGCGTCCTACCTTCGATTGTGGGCTTTGAGCTTGGCCCACTCAGAGTTGTCAACTGTGTTCTACGAGAAAGTCCTTCTCCTCGCCTGGGGGTATGACAGCTTTATCATCCGGTTAATTGGGTTAGCAGTTTTTGCCTTTGCAACTGCCTTCATATTGCTCATGATGGAGACACTCAGCGCTTTTCTTCATGCCTTGCGTCTTCATTGGGTGGAATTTCAAAATAAGTTCTACCTTGGTGATGGGTATAAGTTCAAACCTTTTTCCTTCACCTCCATAGCTACTGAGGATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

704

Amino Acids

80.32

Weight (kDa)

6.31

Isoelectric Point (pI)

39.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 1 - 695 2.3e-268 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1386, 1685
AccB1I GGYRCC 2 cut(s) 1386, 1685
AciI CCGC 1 cut(s) 1400
AclWI GGATC 7 cut(s) 335, 348, 1208, 1229, 1242, 1707, 1712
AcsI RAATTY 4 cut(s) 223, 423, 1276, 2030
AcuI CTGAAG 2 cut(s) 483, 1550
AcyI GRCGYC 1 cut(s) 1820
AfaI GTAC 5 cut(s) 899, 1062, 1208, 1388, 1687
AfeI AGCGCT 1 cut(s) 1996
AfiI CCNNNNNNNGG 1 cut(s) 1903
AflII CTTAAG 1 cut(s) 1510
AflIII ACRYGT 2 cut(s) 642, 1503
AjiI CACGTC 2 cut(s) 509, 1708
AjnI CCWGG 3 cut(s) 45, 585, 1901
AjuI GAANNNNNNNTTGG 2 cut(s) 1548, 1580
AloI GAACNNNNNNTCC 2 cut(s) 966, 998
AluBI AGCT 9 cut(s) 43, 257, 623, 1006, 1029, 1371, 1848, 1917, 2096
AluI AGCT 9 cut(s) 43, 257, 623, 1006, 1029, 1371, 1848, 1917, 2096
Alw21I GWGCWC 4 cut(s) 45, 1015, 1466, 1770
Alw26I GTCTC 2 cut(s) 1080, 1979
Alw44I GTGCAC 2 cut(s) 1462, 1766
AlwI GGATC 7 cut(s) 335, 348, 1208, 1229, 1242, 1707, 1712
Ama87I CYCGRG 1 cut(s) 1022
Aor51HI AGCGCT 1 cut(s) 1996
AoxI GGCC 4 cut(s) 432, 561, 1552, 1851
ApaLI GTGCAC 2 cut(s) 1462, 1766
ApeKI GCWGC 1 cut(s) 1368
ApoI RAATTY 4 cut(s) 223, 423, 1276, 2030
ArsI GACNNNNNNTTYG 2 cut(s) 1805, 1837
Asp700I GAANNNNTTC 2 cut(s) 21, 1255
Asp718I GGTACC 2 cut(s) 1386, 1685
AspLEI GCGC 2 cut(s) 1056, 1997
AspS9I GGNCC 4 cut(s) 200, 562, 1552, 1852
AsuHPI GGTGA 4 cut(s) 575, 789, 2066, 2077
AvaI CYCGRG 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 200
BaeGI GKGCMC 2 cut(s) 1466, 1770
BamHI GGATCC 2 cut(s) 340, 1234
BanI GGYRCC 2 cut(s) 1386, 1685
BanII GRGCYC 1 cut(s) 45
BbsI GAAGAC 2 cut(s) 376, 2009
Bbv12I GWGCWC 4 cut(s) 45, 1015, 1466, 1770
BbvI GCAGC 1 cut(s) 1380
BccI CCATC 6 cut(s) 52, 211, 775, 964, 1975, 2051
BceAI ACGGC 1 cut(s) 1833
BciT130I CCWGG 3 cut(s) 47, 587, 1903
BcoDI GTCTC 2 cut(s) 1080, 1979
BfaI CTAG 4 cut(s) 128, 492, 1239, 1445
BfmI CTRYAG 2 cut(s) 573, 891
BfoI RGCGCY 2 cut(s) 1057, 1998
BfrI CTTAAG 1 cut(s) 1510
BisI GCNGC 2 cut(s) 1369, 1400
BlsI GCNGC 2 cut(s) 1370, 1401
Bme1390I CCNGG 3 cut(s) 47, 587, 1903
Bme18I GGWCC 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 1022
BmgBI CACGTC 2 cut(s) 509, 1708
BmgT120I GGNCC 4 cut(s) 200, 562, 1552, 1852
BmiI GGNNCC 6 cut(s) 201, 342, 1236, 1388, 1553, 1687
BmrFI CCNGG 3 cut(s) 47, 587, 1903
BmrI ACTGGG 1 cut(s) 955
BmsI GCATC 9 cut(s) 264, 526, 775, 844, 1029, 1156, 1171, 1599, 1668
BmuI ACTGGG 1 cut(s) 955
BoxI GACNNNNGTC 1 cut(s) 948
BpiI GAAGAC 2 cut(s) 376, 2009
BplI GAGNNNNNCTC 2 cut(s) 1103, 1135
BpmI CTGGAG 2 cut(s) 68, 200
BsaHI GRCGYC 1 cut(s) 1820
BsaJI CCNNGG 3 cut(s) 1604, 1902, 2050
BsaWI WCCGGW 1 cut(s) 1926
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 966, 996
Bsc4I CCNNNNNNNGG 1 cut(s) 1903
Bse118I RCCGGY 1 cut(s) 1466
Bse1I ACTGG 2 cut(s) 950, 1390
Bse3DI GCAATG 2 cut(s) 173, 725
BseBI CCWGG 3 cut(s) 47, 587, 1903
BseDI CCNNGG 3 cut(s) 1604, 1902, 2050
BseGI GGATG 8 cut(s) 279, 541, 603, 790, 1614, 1683, 1923, 2110
BseLI CCNNNNNNNGG 1 cut(s) 1903
BseMI GCAATG 2 cut(s) 173, 725
BseMII CTCAG 7 cut(s) 405, 1021, 1028, 1354, 1872, 2005, 2091
BseNI ACTGG 2 cut(s) 950, 1390
BseRI GAGGAG 2 cut(s) 1754, 1886
BseSI GKGCMC 2 cut(s) 1466, 1770
BseXI GCAGC 1 cut(s) 1380
BsgI GTGCAG 1 cut(s) 1824
BshFI GGCC 4 cut(s) 434, 563, 1554, 1853
BshNI GGYRCC 2 cut(s) 1386, 1685
BsiHKAI GWGCWC 4 cut(s) 45, 1015, 1466, 1770
BsiHKCI CYCGRG 1 cut(s) 1022
BsiSI CCGG 2 cut(s) 1467, 1927
BslFI GGGAC 4 cut(s) 213, 956, 1109, 1586
BslI CCNNNNNNNGG 1 cut(s) 1903
BsmAI GTCTC 2 cut(s) 1080, 1979
BsmFI GGGAC 4 cut(s) 213, 956, 1109, 1586
BsnI GGCC 4 cut(s) 434, 563, 1554, 1853
BsoBI CYCGRG 1 cut(s) 1022
Bsp1286I GDGCHC 4 cut(s) 45, 1015, 1466, 1770
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
BspACI CCGC 1 cut(s) 1400
BspANI GGCC 4 cut(s) 434, 563, 1554, 1853
BspCNI CTCAG 7 cut(s) 404, 1020, 1027, 1353, 1871, 2004, 2092
BspHI TCATGA 2 cut(s) 1735, 1977
BspLI GGNNCC 6 cut(s) 201, 342, 1236, 1388, 1553, 1687
BspMAI CTGCAG 1 cut(s) 895
BspPI GGATC 7 cut(s) 335, 348, 1208, 1229, 1242, 1707, 1712
BspT107I GGYRCC 2 cut(s) 1386, 1685
BspTI CTTAAG 1 cut(s) 1510
BsrDI GCAATG 2 cut(s) 173, 725
BsrFI RCCGGY 1 cut(s) 1466
BsrGI TGTACA 1 cut(s) 897
BsrI ACTGG 2 cut(s) 950, 1390
BssAI RCCGGY 1 cut(s) 1466
BssECI CCNNGG 3 cut(s) 1604, 1902, 2050
BssMI GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
BssNI GRCGYC 1 cut(s) 1820
BssT1I CCWWGG 2 cut(s) 1604, 2050
Bst2UI CCWGG 3 cut(s) 47, 587, 1903
Bst4CI ACNGT 7 cut(s) 373, 904, 1094, 1192, 1359, 1421, 1873
Bst6I CTCTTC 1 cut(s) 126
BstACI GRCGYC 1 cut(s) 1820
BstAFI CTTAAG 1 cut(s) 1510
BstAPI GCANNNNNTGC 2 cut(s) 890, 1949
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 1 cut(s) 402
BstF5I GGATG 8 cut(s) 279, 541, 603, 790, 1614, 1683, 1923, 2110
BstH2I RGCGCY 2 cut(s) 1057, 1998
BstHHI GCGC 2 cut(s) 1056, 1997
BstKTI GATC 9 cut(s) 211, 343, 1216, 1237, 1410, 1528, 1572, 1702, 1720
BstMAI GTCTC 2 cut(s) 1080, 1979
BstMBI GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
BstMWI GCNNNNNNNGC 4 cut(s) 440, 890, 1595, 1949
BstNI CCWGG 3 cut(s) 47, 587, 1903
BstNSI RCATGY 2 cut(s) 646, 1507
BstPAI GACNNNNGTC 1 cut(s) 948
BstSCI CCNGG 3 cut(s) 45, 585, 1901
BstSFI CTRYAG 2 cut(s) 573, 891
BstSLI GKGCMC 2 cut(s) 1466, 1770
BstV1I GCAGC 1 cut(s) 1380
BstV2I GAAGAC 2 cut(s) 376, 2009
BstX2I RGATCY 4 cut(s) 340, 1213, 1234, 1699
BstYI RGATCY 4 cut(s) 340, 1213, 1234, 1699
BsuRI GGCC 4 cut(s) 434, 563, 1554, 1853
BtrI CACGTC 2 cut(s) 509, 1708
BtsCI GGATG 8 cut(s) 279, 541, 603, 790, 1614, 1683, 1923, 2110
BtsI GCAGTG 2 cut(s) 900, 1183
BtsIMutI CAGTG 7 cut(s) 174, 900, 900, 1015, 1183, 1188, 1759
Cac8I GCNNGC 1 cut(s) 402
CciI TCATGA 2 cut(s) 1735, 1977
CfoI GCGC 2 cut(s) 1056, 1997
Cfr10I RCCGGY 1 cut(s) 1466
Cfr13I GGNCC 4 cut(s) 200, 562, 1552, 1852
CseI GACGC 3 cut(s) 1631, 1809, 2003
Csp6I GTAC 5 cut(s) 898, 1061, 1207, 1387, 1686
CviQI GTAC 5 cut(s) 898, 1061, 1207, 1387, 1686
DpnI GATC 9 cut(s) 210, 342, 1215, 1236, 1409, 1527, 1571, 1701, 1719
DpnII GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
Eam1104I CTCTTC 1 cut(s) 126
EarI CTCTTC 1 cut(s) 126
Ecl136II GAGCTC 1 cut(s) 43
Eco130I CCWWGG 2 cut(s) 1604, 2050
Eco147I AGGCCT 1 cut(s) 434
Eco24I GRGCYC 1 cut(s) 45
Eco32I GATATC 3 cut(s) 190, 875, 1433
Eco47I GGWCC 1 cut(s) 200
Eco47III AGCGCT 1 cut(s) 1996
Eco53kI GAGCTC 1 cut(s) 43
Eco57I CTGAAG 2 cut(s) 483, 1550
Eco88I CYCGRG 1 cut(s) 1022
EcoICRI GAGCTC 1 cut(s) 43
EcoO109I RGGNCCY 1 cut(s) 562
EcoRI GAATTC 1 cut(s) 1276
EcoRII CCWGG 3 cut(s) 45, 585, 1901
EcoRV GATATC 3 cut(s) 190, 875, 1433
EcoT14I CCWWGG 2 cut(s) 1604, 2050
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 1 cut(s) 45
ErhI CCWWGG 2 cut(s) 1604, 2050
FaqI GGGAC 4 cut(s) 213, 956, 1109, 1586
FauNDI CATATG 2 cut(s) 859, 1672
Fnu4HI GCNGC 2 cut(s) 1369, 1400
FokI GGATG 7 cut(s) 286, 548, 610, 797, 1621, 1690, 1910
FriOI GRGCYC 1 cut(s) 45
Fsp4HI GCNGC 2 cut(s) 1369, 1400
FspBI CTAG 4 cut(s) 128, 492, 1239, 1445
GlaI GCGC 2 cut(s) 1055, 1996
GluI GCNGC 2 cut(s) 1369, 1400
GsuI CTGGAG 2 cut(s) 68, 200
HaeII RGCGCY 2 cut(s) 1057, 1998
HaeIII GGCC 4 cut(s) 434, 563, 1554, 1853
HapII CCGG 2 cut(s) 1467, 1927
HgaI GACGC 3 cut(s) 1631, 1809, 2003
HhaI GCGC 2 cut(s) 1056, 1997
Hin1I GRCGYC 1 cut(s) 1820
Hin6I GCGC 2 cut(s) 1054, 1995
HinP1I GCGC 2 cut(s) 1054, 1995
HincII GTYRAC 1 cut(s) 1869
HindII GTYRAC 1 cut(s) 1869
HinfI GANTC 5 cut(s) 164, 415, 544, 715, 794
HpaII CCGG 2 cut(s) 1467, 1927
HphI GGTGA 4 cut(s) 575, 789, 2066, 2077
Hpy166II GTNNAC 6 cut(s) 142, 898, 900, 1464, 1768, 1869
Hpy188I TCNGA 8 cut(s) 194, 208, 420, 1017, 1261, 1569, 1693, 1861
Hpy188III TCNNGA 9 cut(s) 55, 461, 719, 842, 878, 1024, 1703, 1736, 1978
Hpy8I GTNNAC 6 cut(s) 142, 898, 900, 1464, 1768, 1869
HpyAV CCTTC 6 cut(s) 606, 677, 1838, 1901, 1975, 2092
HpyCH4III ACNGT 7 cut(s) 373, 904, 1094, 1192, 1359, 1421, 1873
HpyCH4IV ACGT 4 cut(s) 508, 733, 1326, 1707
HpyF10VI GCNNNNNNNGC 4 cut(s) 440, 890, 1595, 1949
HpySE526I ACGT 4 cut(s) 508, 733, 1326, 1707
Hsp92I GRCGYC 1 cut(s) 1820
HspAI GCGC 2 cut(s) 1054, 1995
KpnI GGTACC 2 cut(s) 1390, 1689
Kzo9I GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
LmnI GCTCC 4 cut(s) 48, 181, 974, 1026
Lsp1109I GCAGC 1 cut(s) 1380
LweI GCATC 9 cut(s) 264, 526, 775, 844, 1029, 1156, 1171, 1599, 1668
MaeI CTAG 4 cut(s) 128, 492, 1239, 1445
MaeII ACGT 4 cut(s) 508, 733, 1326, 1707
MaeIII GTNAC 4 cut(s) 109, 658, 1343, 1578
MalI GATC 9 cut(s) 210, 342, 1215, 1236, 1409, 1527, 1571, 1701, 1719
MboI GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
MboII GAAGA 8 cut(s) 143, 376, 379, 476, 1108, 1402, 1994, 2009
MfeI CAATTG 1 cut(s) 1374
MflI RGATCY 4 cut(s) 340, 1213, 1234, 1699
MhlI GDGCHC 4 cut(s) 45, 1015, 1466, 1770
MmeI TCCRAC 2 cut(s) 658, 1541
Mph1103I ATGCAT 1 cut(s) 859
MroXI GAANNNNTTC 2 cut(s) 21, 1255
MseI TTAA 7 cut(s) 647, 1070, 1199, 1416, 1511, 1746, 1931
MspCI CTTAAG 1 cut(s) 1510
MspI CCGG 2 cut(s) 1467, 1927
MspR9I CCNGG 3 cut(s) 47, 587, 1903
MunI CAATTG 1 cut(s) 1374
MvaI CCWGG 3 cut(s) 47, 587, 1903
MwoI GCNNNNNNNGC 4 cut(s) 440, 890, 1595, 1949
NdeI CATATG 2 cut(s) 859, 1672
NdeII GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
NlaIV GGNNCC 6 cut(s) 201, 342, 1236, 1388, 1553, 1687
NmuCI GTSAC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 2 cut(s) 646, 1507
PagI TCATGA 2 cut(s) 1735, 1977
PceI AGGCCT 1 cut(s) 434
PciI ACATGT 2 cut(s) 642, 1503
PdmI GAANNNNTTC 2 cut(s) 21, 1255
PfeI GAWTC 5 cut(s) 164, 415, 544, 715, 794
PfoI TCCNGGA 1 cut(s) 45
PkrI GCNGC 2 cut(s) 1370, 1401
PscI ACATGT 2 cut(s) 642, 1503
PshAI GACNNNNGTC 1 cut(s) 948
Psp124BI GAGCTC 1 cut(s) 45
Psp6I CCWGG 3 cut(s) 45, 585, 1901
PspGI CCWGG 3 cut(s) 45, 585, 1901
PspN4I GGNNCC 6 cut(s) 201, 342, 1236, 1388, 1553, 1687
PspPI GGNCC 4 cut(s) 200, 562, 1552, 1852
PstI CTGCAG 1 cut(s) 895
PsuI RGATCY 4 cut(s) 340, 1213, 1234, 1699
RsaI GTAC 5 cut(s) 899, 1062, 1208, 1388, 1687
RsaNI GTAC 5 cut(s) 898, 1061, 1207, 1387, 1686
SacI GAGCTC 1 cut(s) 45
SaqAI TTAA 7 cut(s) 647, 1070, 1199, 1416, 1511, 1746, 1931
SatI GCNGC 2 cut(s) 1369, 1400
Sau3AI GATC 9 cut(s) 208, 340, 1213, 1234, 1407, 1525, 1569, 1699, 1717
Sau96I GGNCC 4 cut(s) 200, 562, 1552, 1852
ScrFI CCNGG 3 cut(s) 47, 587, 1903
SduI GDGCHC 4 cut(s) 45, 1015, 1466, 1770
SfaNI GCATC 9 cut(s) 264, 526, 775, 844, 1029, 1156, 1171, 1599, 1668
SfcI CTRYAG 2 cut(s) 573, 891
SinI GGWCC 1 cut(s) 200
SmlI CTYRAG 1 cut(s) 1510
SmoI CTYRAG 1 cut(s) 1510
SseBI AGGCCT 1 cut(s) 434
SsiI CCGC 1 cut(s) 1400
SspI AATATT 1 cut(s) 771
SspMI CTAG 4 cut(s) 128, 492, 1239, 1445
SstI GAGCTC 1 cut(s) 45
StuI AGGCCT 1 cut(s) 434
StyD4I CCNGG 3 cut(s) 45, 585, 1901
StyI CCWWGG 2 cut(s) 1604, 2050
TaaI ACNGT 7 cut(s) 373, 904, 1094, 1192, 1359, 1421, 1873
TaiI ACGT 4 cut(s) 511, 736, 1329, 1710
TaqI TCGA 2 cut(s) 25, 1831
TatI WGTACW 3 cut(s) 897, 1060, 1206
TauI GCSGC 1 cut(s) 1402
TfiI GAWTC 5 cut(s) 164, 415, 544, 715, 794
Tru1I TTAA 7 cut(s) 647, 1070, 1199, 1416, 1511, 1746, 1931
Tru9I TTAA 7 cut(s) 647, 1070, 1199, 1416, 1511, 1746, 1931
TscAI CASTG 7 cut(s) 174, 900, 907, 1015, 1183, 1195, 1759
TseFI GTSAC 1 cut(s) 109
TseI GCWGC 1 cut(s) 1368
Tsp45I GTSAC 1 cut(s) 109
TspRI CASTG 7 cut(s) 174, 900, 907, 1015, 1183, 1195, 1759
Vha464I CTTAAG 1 cut(s) 1510
VneI GTGCAC 2 cut(s) 1462, 1766
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 4 cut(s) 223, 423, 1276, 2030
XceI RCATGY 2 cut(s) 646, 1507
XmnI GAANNNNTTC 2 cut(s) 21, 1255
XspI CTAG 4 cut(s) 128, 492, 1239, 1445
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.