MD03G1109800.v1.1

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009092
Physical Location & Seq
Reverse (-)
9537830 .. 9549121
11292 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1109800.v1.1.491

Sequence Viewer

Length: 2460 bp
ATGGAGAAGTTCATCGACAACTTACCCGCCATGGATCTGATGCGCTCCGAGAAGATGACCTTCGTCCAGCTCATCATCCCCGTCGAGTCCGCCCACCGCGCCATTTCTTACCTCGGCGAACTCGGCCTCCTCCAATTCCGAGACTTAAATGCTGATAAAAGTCCTTTCCAACGAACATTTGTTAATCAGGTAAAGCGATGTGCGGAGATGTCAAGAAAGCTGCGGTTTTTCAGAGATCAAATTAGTAAAGCTGGTCTACTTTCATCTGTACATCCTGTTTTACAACCAGATATTGACTTAGAGGAATTAGAGATACAATTAGCCGAGCATGAGCATGAGCTTATTGAGTTGAACTCTAATAGCGATAGAATTCAACATTCATACAATGAACTCCTCGAGTACAAGATGGTATTGCAAAAGGCAAGTGGCTTTCTTGTTTCAAGTAATAGCCATGCGGCTTCGGAGGAGAGAGAACTAGATGAGAATATTTACTCAAATGATCATTATGGTGATGAAGTATCATTACTTGAGCAGGATATTAGACCTGGACCATCTGACCAATCTGGTTTGAGATTTGTTAGTGGAATTATTTGTAAGTCCAAGGCTCTCAGATTTGAGAGGATGTTGTTTCGTGCTACAAGGGGCAACATGCTTTTCAACCATGCCCCAGCAGATGAACTGATGATGGACCCTTTATCTACTGAAATGGTTGAGAAAACAGTTTTTGTAGTGTTCTTCTCTGGGATGCAGGCAAAAACAAAGATTTTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTATCCTGTTCCTGAAGATACAACTAGGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAACAACCTTGGATGCAGGGATTCGTCACAGAAATAAGGCCCTTGCATCTGTAGGGTTTCACCTAGCAAAATGGATGAACATGGTAAGAAGGGAGAAAGCTGTTTATGATACATTGAACATGCTAAACTTTGATGTTACAAAAAAATGTCTTGTTGGAGAGGGCTGGTGTCCTATATTTGCGAAACCTAAGATTCAGGAGGCACTGCAACGTGCTACATTTGATAGCAATTCGCAAGTGGGAGTAATATTTCATGTGATGGATGCAATAGATTCACCTCCTACATATTTCAGAACCAACCGCTTCACAAGTGCATTTCAGGAAATTGTTGATGCATACGGTGTTGCAAGATATCAAGAAGCCAACCCTGCAGTTTACACTGTTATTACATTTCCGTTTCTTTTTGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTATTGCTTGGAGCATTAGTTCTTATAGCTCGTGAAAGTAAACTCAGTGCACAGAAACTTGGGAGCTTTATGGAGATGTTATTTGGTGGACGATATGTACTTCTCTTGATGTCCCTATTTTCAATCTACTGTGGGTTGATTTACAATGAGTTCTTTTCTGTTCCTTTTCACATATTTGGCGGGTCTGCTTACAAATGTAGAGATACTGCTTGCAGTGAGGTGCACACTATTGGCTTAATCAAGTACCGAGATCCATACCCATTTGGTGTGGATCCCAGTTGGCGTGGAAGTCGTTCTGAACTGCCTTTTCTGAATTCTCTCAAAATGAAAATGTCAATTTTGTTGGGTGTTGTGCAAATGAACTTGGGAATACTACTAAGTTACTTCAATGCAAGGTTTTTCAGCAGCTCGCTTGATATATGGTACCAGTTTGTCCCACAGATGATCTTTCTTAACAGTCTTTTTGGATATCTTTCACTTCTTGTTGTCATCAAGTGGTGCACGGGATCTCAGGCAGACCTCTATCATGTAATGATTTACATGTTTTTAAGTCCAACCGACGATCTTGGTGAAAATCAATTATTCTGGGGCCAAAGACCACTTCAGATCATTTTGTTGCTTTTGGCTTTAATTGCAGTTCCATGGATGCTCTTTCCAAAACCTTTTATTTTGAAGAAGCTTCATACGGAGAGGTTTCAAGGTCGCGCGTATGGGATGCTTGGCACCTCAGAGATGGATCTTGAGGTGGAACCTGATTCTGCGAGGCAACATCATGAAGAATTTAATTTCAGTGAGGTATTTGTACACCAGATGATACACTCCATAGAGTTTGTTCTGGGTGCTGTTTCAAATACGGCTTCATATCTACGACTATGGGCTTTGAGCTTGGCGCACTCAGAATTGTCGACCGTTTTCTATGAGAAAGTTCTCCTCCTTGCTTGGGGGTATGACAGCTTCATTATCCGGTTAATTGGGTTATCAGTTTTCGCCTTTGCAACTGCATTCATACTACTCATGATGGAGACACTAAGTGCTTTTCTCCATGCCCTGCGTCTTCATTGGGTAGAATATCAAAACAAATTCTACCACGGAGATGGCTACAAGTTCAAACCTTTTTCCTTTGCCTCCATAACCGAGGATGAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

820

Amino Acids

93.81

Weight (kDa)

5.98

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 37 - 811 2e-294 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1734
AccB1I GGYRCC 2 cut(s) 1734, 2033
AccI GTMKAC 2 cut(s) 256, 2216
AccII CGCG 3 cut(s) 99, 2016, 2018
AciI CCGC 8 cut(s) 27, 90, 97, 203, 223, 455, 1171, 1491
AclWI GGATC 6 cut(s) 42, 1556, 1577, 1590, 1825, 2055
AcsI RAATTY 5 cut(s) 369, 771, 1624, 2090, 2390
AcuI CTGAAG 2 cut(s) 831, 1898
AdeI CACNNNGTG 1 cut(s) 2342
AfaI GTAC 6 cut(s) 270, 401, 1410, 1556, 1736, 2115
AfiI CCNNNNNNNGG 1 cut(s) 2251
AflIII ACRYGT 1 cut(s) 1851
AjiI CACGTC 1 cut(s) 857
AjnI CCWGG 1 cut(s) 544
AjuI GAANNNNNNNTTGG 2 cut(s) 1896, 1928
AloI GAACNNNNNNTCC 4 cut(s) 111, 143, 1314, 1346
Alw21I GWGCWC 3 cut(s) 1363, 1536, 1814
Alw26I GTCTC 2 cut(s) 135, 2327
Alw44I GTGCAC 3 cut(s) 1359, 1532, 1810
AlwI GGATC 6 cut(s) 42, 1556, 1577, 1590, 1825, 2055
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 4 cut(s) 124, 780, 909, 1900
ApaLI GTGCAC 3 cut(s) 1359, 1532, 1810
ApeKI GCWGC 2 cut(s) 220, 1716
ApoI RAATTY 5 cut(s) 369, 771, 1624, 2090, 2390
Asp700I GAANNNNTTC 1 cut(s) 1603
Asp718I GGTACC 1 cut(s) 1734
AspLEI GCGC 4 cut(s) 45, 101, 2018, 2203
AspS9I GGNCC 4 cut(s) 548, 688, 910, 1900
AsuHPI GGTGA 4 cut(s) 521, 923, 1137, 1892
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 2 cut(s) 548, 688
BaeGI GKGCMC 3 cut(s) 1363, 1536, 1814
BamHI GGATCC 1 cut(s) 1582
BanI GGYRCC 2 cut(s) 1734, 2033
BarI GAAGNNNNNNTAC 4 cut(s) 507, 539, 2249, 2281
BauI CACGAG 1 cut(s) 1341
BbsI GAAGAC 1 cut(s) 2357
Bbv12I GWGCWC 3 cut(s) 1363, 1536, 1814
BbvI GCAGC 2 cut(s) 207, 1728
BccI CCATC 7 cut(s) 400, 559, 679, 1123, 2038, 2323, 2399
BceAI ACGGC 1 cut(s) 2181
BciT130I CCWGG 1 cut(s) 546
BclI TGATCA 1 cut(s) 499
BcoDI GTCTC 2 cut(s) 135, 2327
BfaI CTAG 4 cut(s) 476, 822, 840, 935
BfmI CTRYAG 2 cut(s) 921, 1239
BisI GCNGC 3 cut(s) 221, 456, 1717
BlsI GCNGC 3 cut(s) 222, 457, 1718
Bme1390I CCNGG 1 cut(s) 546
Bme18I GGWCC 2 cut(s) 548, 688
BmeT110I CYCGRG 1 cut(s) 395
BmgBI CACGTC 1 cut(s) 857
BmgT120I GGNCC 4 cut(s) 548, 688, 910, 1900
BmiI GGNNCC 6 cut(s) 690, 1584, 1736, 1901, 2035, 2061
BmrFI CCNGG 1 cut(s) 546
BmrI ACTGGG 2 cut(s) 1303, 1581
BmsI GCATC 8 cut(s) 30, 735, 874, 926, 1123, 1192, 1947, 2016
BmuI ACTGGG 2 cut(s) 1303, 1581
BoxI GACNNNNGTC 2 cut(s) 62, 1296
BpiI GAAGAC 1 cut(s) 2357
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpuEI CTTGAG 2 cut(s) 548, 2072
BsaJI CCNNGG 7 cut(s) 30, 112, 600, 879, 1952, 2398, 2445
BsaWI WCCGGW 1 cut(s) 2274
BsaXI ACNNNNNCTCC 4 cut(s) 111, 141, 1314, 1344
Bsc4I CCNNNNNNNGG 1 cut(s) 2251
Bse1I ACTGG 3 cut(s) 1298, 1587, 1738
BseBI CCWGG 1 cut(s) 546
BseDI CCNNGG 7 cut(s) 30, 112, 600, 879, 1952, 2398, 2445
BseLI CCNNNNNNNGG 1 cut(s) 2251
BseMII CTCAG 5 cut(s) 622, 1369, 1835, 2052, 2220
BseNI ACTGG 3 cut(s) 1298, 1587, 1738
BseRI GAGGAG 4 cut(s) 119, 383, 479, 2231
BseSI GKGCMC 3 cut(s) 1363, 1536, 1814
BseXI GCAGC 2 cut(s) 207, 1728
BseYI CCCAGC 1 cut(s) 667
Bsh1236I CGCG 3 cut(s) 99, 2016, 2018
Bsh1285I CGRYCG 1 cut(s) 2220
BshFI GGCC 4 cut(s) 126, 782, 911, 1902
BshNI GGYRCC 2 cut(s) 1734, 2033
BsiEI CGRYCG 1 cut(s) 2220
BsiHKAI GWGCWC 3 cut(s) 1363, 1536, 1814
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 2275
BslFI GGGAC 3 cut(s) 1304, 1408, 1730
BslI CCNNNNNNNGG 1 cut(s) 2251
BsmAI GTCTC 2 cut(s) 135, 2327
BsmFI GGGAC 3 cut(s) 1304, 1408, 1730
BsmI GAATGC 1 cut(s) 2312
BsnI GGCC 4 cut(s) 126, 782, 911, 1902
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 3 cut(s) 1363, 1536, 1814
Bsp1407I TGTACA 2 cut(s) 268, 2113
Bsp19I CCATGG 2 cut(s) 30, 1952
BspACI CCGC 8 cut(s) 27, 90, 97, 203, 223, 455, 1171, 1491
BspANI GGCC 4 cut(s) 126, 782, 911, 1902
BspCNI CTCAG 5 cut(s) 621, 1368, 1834, 2051, 2219
BspFNI CGCG 3 cut(s) 99, 2016, 2018
BspHI TCATGA 2 cut(s) 2083, 2325
BspLI GGNNCC 6 cut(s) 690, 1584, 1736, 1901, 2035, 2061
BspMAI CTGCAG 1 cut(s) 1243
BspPI GGATC 6 cut(s) 42, 1556, 1577, 1590, 1825, 2055
BspT107I GGYRCC 2 cut(s) 1734, 2033
BsrGI TGTACA 2 cut(s) 268, 2113
BsrI ACTGG 3 cut(s) 1298, 1587, 1738
BssECI CCNNGG 7 cut(s) 30, 112, 600, 879, 1952, 2398, 2445
BssSI CACGAG 1 cut(s) 1341
BssT1I CCWWGG 4 cut(s) 30, 600, 879, 1952
Bst2BI CACGAG 1 cut(s) 1341
Bst2UI CCWGG 1 cut(s) 546
Bst4CI ACNGT 6 cut(s) 721, 1211, 1252, 1442, 1769, 2221
BstAUI TGTACA 2 cut(s) 268, 2113
BstC8I GCNNGC 3 cut(s) 750, 1522, 1721
BstDEI CTNAG 9 cut(s) 298, 608, 1059, 1355, 1688, 1821, 2038, 2206, 2339
BstDSI CCRYGG 3 cut(s) 30, 1952, 2398
BstFNI CGCG 3 cut(s) 99, 2016, 2018
BstHHI GCGC 4 cut(s) 45, 101, 2018, 2203
BstMAI GTCTC 2 cut(s) 135, 2327
BstMCI CGRYCG 1 cut(s) 2220
BstMWI GCNNNNNNNGC 5 cut(s) 98, 123, 788, 1238, 1943
BstNI CCWGG 1 cut(s) 546
BstNSI RCATGY 3 cut(s) 652, 994, 1855
BstPAI GACNNNNGTC 2 cut(s) 62, 1296
BstSCI CCNGG 1 cut(s) 544
BstSFI CTRYAG 2 cut(s) 921, 1239
BstSLI GKGCMC 3 cut(s) 1363, 1536, 1814
BstUI CGCG 3 cut(s) 99, 2016, 2018
BstV1I GCAGC 2 cut(s) 207, 1728
BstV2I GAAGAC 1 cut(s) 2357
BstX2I RGATCY 5 cut(s) 34, 1561, 1582, 1817, 2047
BstXI CCANNNNNNTGG 1 cut(s) 2405
BstYI RGATCY 5 cut(s) 34, 1561, 1582, 1817, 2047
BsuRI GGCC 4 cut(s) 126, 782, 911, 1902
BtgI CCRYGG 3 cut(s) 30, 1952, 2398
BtgZI GCGATG 1 cut(s) 211
BtrI CACGTC 1 cut(s) 857
BtsI GCAGTG 2 cut(s) 1073, 1531
BtsIMutI CAGTG 5 cut(s) 1073, 1248, 1363, 1531, 2107
Cac8I GCNNGC 3 cut(s) 750, 1522, 1721
CciI TCATGA 2 cut(s) 2083, 2325
CfoI GCGC 4 cut(s) 45, 101, 2018, 2203
Cfr13I GGNCC 4 cut(s) 548, 688, 910, 1900
CseI GACGC 1 cut(s) 2351
Csp6I GTAC 6 cut(s) 269, 400, 1409, 1555, 1735, 2114
CviQI GTAC 6 cut(s) 269, 400, 1409, 1555, 1735, 2114
DdeI CTNAG 9 cut(s) 298, 608, 1059, 1355, 1688, 1821, 2038, 2206, 2339
DraIII CACNNNGTG 1 cut(s) 2342
EciI GGCGGA 1 cut(s) 79
Eco130I CCWWGG 4 cut(s) 30, 600, 879, 1952
Eco147I AGGCCT 1 cut(s) 782
Eco32I GATATC 2 cut(s) 1223, 1781
Eco47I GGWCC 2 cut(s) 548, 688
Eco57I CTGAAG 2 cut(s) 831, 1898
Eco88I CYCGRG 1 cut(s) 395
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 2 cut(s) 369, 1624
EcoRII CCWGG 1 cut(s) 544
EcoRV GATATC 2 cut(s) 1223, 1781
EcoT14I CCWWGG 4 cut(s) 30, 600, 879, 1952
EcoT22I ATGCAT 1 cut(s) 1207
ErhI CCWWGG 4 cut(s) 30, 600, 879, 1952
FalI AAGNNNNNCTT 2 cut(s) 44, 76
FaqI GGGAC 3 cut(s) 1304, 1408, 1730
FauI CCCGC 2 cut(s) 34, 1484
FbaI TGATCA 1 cut(s) 499
FblI GTMKAC 2 cut(s) 256, 2216
Fnu4HI GCNGC 3 cut(s) 221, 456, 1717
FokI GGATG 9 cut(s) 62, 258, 634, 757, 896, 958, 1145, 1969, 2038
Fsp4HI GCNGC 3 cut(s) 221, 456, 1717
FspBI CTAG 4 cut(s) 476, 822, 840, 935
GlaI GCGC 4 cut(s) 44, 100, 2017, 2202
GluI GCNGC 3 cut(s) 221, 456, 1717
GsaI CCCAGC 1 cut(s) 671
HaeIII GGCC 4 cut(s) 126, 782, 911, 1902
HapII CCGG 1 cut(s) 2275
HgaI GACGC 1 cut(s) 2351
HhaI GCGC 4 cut(s) 45, 101, 2018, 2203
Hin6I GCGC 4 cut(s) 43, 99, 2016, 2201
HinP1I GCGC 4 cut(s) 43, 99, 2016, 2201
HincII GTYRAC 1 cut(s) 2217
HindII GTYRAC 1 cut(s) 2217
HindIII AAGCTT 1 cut(s) 1988
HinfI GANTC 5 cut(s) 86, 892, 1063, 1142, 2066
HpaII CCGG 1 cut(s) 2275
HphI GGTGA 4 cut(s) 521, 923, 1137, 1892
Hpy166II GTNNAC 9 cut(s) 257, 1246, 1352, 1361, 1400, 1534, 1812, 2116, 2217
Hpy8I GTNNAC 9 cut(s) 257, 1246, 1352, 1361, 1400, 1534, 1812, 2116, 2217
Hpy99I CGWCG 2 cut(s) 86, 1874
HpyAV CCTTC 2 cut(s) 70, 954
HpyCH4III ACNGT 6 cut(s) 721, 1211, 1252, 1442, 1769, 2221
HpyCH4IV ACGT 2 cut(s) 856, 1081
HpyF10VI GCNNNNNNNGC 5 cut(s) 98, 123, 788, 1238, 1943
HpyF3I CTNAG 9 cut(s) 298, 608, 1059, 1355, 1688, 1821, 2038, 2206, 2339
HpySE526I ACGT 2 cut(s) 856, 1081
HspAI GCGC 4 cut(s) 43, 99, 2016, 2201
KpnI GGTACC 1 cut(s) 1738
Ksp22I TGATCA 1 cut(s) 499
LmnI GCTCC 3 cut(s) 50, 1322, 1374
Lsp1109I GCAGC 2 cut(s) 207, 1728
LweI GCATC 8 cut(s) 30, 735, 874, 926, 1123, 1192, 1947, 2016
MaeI CTAG 4 cut(s) 476, 822, 840, 935
MaeII ACGT 2 cut(s) 856, 1081
MaeIII GTNAC 3 cut(s) 896, 1006, 1691
MboII GAAGA 6 cut(s) 64, 727, 824, 1996, 2099, 2357
MflI RGATCY 5 cut(s) 34, 1561, 1582, 1817, 2047
MhlI GDGCHC 3 cut(s) 1363, 1536, 1814
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 3 cut(s) 193, 1006, 1889
Mph1103I ATGCAT 1 cut(s) 1207
MroXI GAANNNNTTC 1 cut(s) 1603
MseI TTAA 8 cut(s) 146, 183, 1547, 1764, 1859, 1940, 2094, 2279
MslI CAYNNNNRTG 3 cut(s) 333, 507, 2403
MspI CCGG 1 cut(s) 2275
MspR9I CCNGG 1 cut(s) 546
Mva1269I GAATGC 1 cut(s) 2312
MvaI CCWGG 1 cut(s) 546
MvnI CGCG 3 cut(s) 99, 2016, 2018
MwoI GCNNNNNNNGC 5 cut(s) 98, 123, 788, 1238, 1943
NcoI CCATGG 2 cut(s) 30, 1952
NlaIV GGNNCC 6 cut(s) 690, 1584, 1736, 1901, 2035, 2061
NmeAIII GCCGAG 3 cut(s) 93, 102, 349
NmuCI GTSAC 1 cut(s) 896
NsiI ATGCAT 1 cut(s) 1207
NspI RCATGY 3 cut(s) 652, 994, 1855
PaeR7I CTCGAG 1 cut(s) 395
PagI TCATGA 2 cut(s) 2083, 2325
PceI AGGCCT 1 cut(s) 782
PciI ACATGT 1 cut(s) 1851
PctI GAATGC 1 cut(s) 2312
PdmI GAANNNNTTC 1 cut(s) 1603
PfeI GAWTC 4 cut(s) 892, 1063, 1142, 2066
PkrI GCNGC 3 cut(s) 222, 457, 1718
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PscI ACATGT 1 cut(s) 1851
PshAI GACNNNNGTC 2 cut(s) 62, 1296
Psp6I CCWGG 1 cut(s) 544
PspFI CCCAGC 1 cut(s) 667
PspGI CCWGG 1 cut(s) 544
PspN4I GGNNCC 6 cut(s) 690, 1584, 1736, 1901, 2035, 2061
PspPI GGNCC 4 cut(s) 548, 688, 910, 1900
PspXI VCTCGAGB 1 cut(s) 395
PstI CTGCAG 1 cut(s) 1243
PsuI RGATCY 5 cut(s) 34, 1561, 1582, 1817, 2047
RsaI GTAC 6 cut(s) 270, 401, 1410, 1556, 1736, 2115
RsaNI GTAC 6 cut(s) 269, 400, 1409, 1555, 1735, 2114
RseI CAYNNNNRTG 3 cut(s) 333, 507, 2403
SalI GTCGAC 1 cut(s) 2215
SaqAI TTAA 8 cut(s) 146, 183, 1547, 1764, 1859, 1940, 2094, 2279
SatI GCNGC 3 cut(s) 221, 456, 1717
Sau96I GGNCC 4 cut(s) 548, 688, 910, 1900
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 546
SduI GDGCHC 3 cut(s) 1363, 1536, 1814
SfaNI GCATC 8 cut(s) 30, 735, 874, 926, 1123, 1192, 1947, 2016
SfcI CTRYAG 2 cut(s) 921, 1239
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 2 cut(s) 548, 688
SlaI CTCGAG 1 cut(s) 395
SmiMI CAYNNNNRTG 3 cut(s) 333, 507, 2403
SmlI CTYRAG 3 cut(s) 395, 527, 2051
SmoI CTYRAG 3 cut(s) 395, 527, 2051
SseBI AGGCCT 1 cut(s) 782
SsiI CCGC 8 cut(s) 27, 90, 97, 203, 223, 455, 1171, 1491
SspI AATATT 2 cut(s) 487, 1119
SspMI CTAG 4 cut(s) 476, 822, 840, 935
StuI AGGCCT 1 cut(s) 782
StyD4I CCNGG 1 cut(s) 544
StyI CCWWGG 4 cut(s) 30, 600, 879, 1952
TaaI ACNGT 6 cut(s) 721, 1211, 1252, 1442, 1769, 2221
TaiI ACGT 2 cut(s) 859, 1084
TaqI TCGA 4 cut(s) 15, 84, 396, 2216
TatI WGTACW 4 cut(s) 268, 399, 1408, 2113
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 4 cut(s) 892, 1063, 1142, 2066
Tru1I TTAA 8 cut(s) 146, 183, 1547, 1764, 1859, 1940, 2094, 2279
Tru9I TTAA 8 cut(s) 146, 183, 1547, 1764, 1859, 1940, 2094, 2279
TscAI CASTG 5 cut(s) 1080, 1255, 1363, 1531, 2107
TseFI GTSAC 1 cut(s) 896
TseI GCWGC 2 cut(s) 220, 1716
Tsp45I GTSAC 1 cut(s) 896
TspGWI ACGGA 3 cut(s) 1254, 2012, 2415
TspRI CASTG 5 cut(s) 1080, 1255, 1363, 1531, 2107
VneI GTGCAC 3 cut(s) 1359, 1532, 1810
VpaK11BI GGWCC 2 cut(s) 548, 688
XapI RAATTY 5 cut(s) 369, 771, 1624, 2090, 2390
XceI RCATGY 3 cut(s) 652, 994, 1855
XhoI CTCGAG 1 cut(s) 395
XmiI GTMKAC 2 cut(s) 256, 2216
XmnI GAANNNNTTC 1 cut(s) 1603
XspI CTAG 4 cut(s) 476, 822, 840, 935
Zsp2I ATGCAT 1 cut(s) 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.