Rh5AG400300

Essential component of the vacuolar proton pump (V- ATPase), a multimeric enzyme that catalyzes the translocation of protons across the membranes. Required for assembly and activity of the V-ATPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0009092
Physical Location & Seq
Forward (+)
69325614 .. 69335624
10011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG400300.1

Sequence Viewer

Length: 2595 bp
ATGGAGAAGTTCCTGGACAAGTTGCCCTCCATGGATCTGATGCGCTCCGAGAAGATGACCTTCGTCCAGCTCATCATCCCCGTCGAGTCTGCTCACCGCACCATTTCCTATCTCGGCGAAATCGGCCTCCTCCAATTCCGTGACATCACCTCCTTTGGTGTCTGGGATTCGCATACATCAGTTTTTGAAGGAGTTTTGAGCACCACTTTACTTGACAAGTCAACCAGTAGATGGGTATTCTGCACACCGATATTTAATGCGGATTGTTTTGCAATTTTAAATGCTGATAAAAGCCCTTTCCAGCTAACCTTTGTTAATCAGGTGAAGCGATGTGCAGAGATGTCAAGAAAGCTGCGCTTTTTTAAGGATCAAATCAGTAAAGCTGGTCTGCTTGGGTCTATACGTCCAGTTTCACAACCAGATATCGAGTTGGAGGAATTAGAGTTTCAACTTGGTGAGCACGAACATGAGCTAATTGAAATGAACTCTAATAGTGAAAGACTTCGACAATCATACAATGAGCTCCTGGAGTTCAAGATGGTATTACAAAAGGCAAGTGGCTTTCTTGTTTCAAGTAATAGCCATGCTGTCGCAGAGGAAACAGAACTAGAAGAGAATGTTTACTCAATGAATGATTATGGTGATTCAGTGTCATTGCTGGAGCAGGATATCAGACGGGGATCATCAGATCAATCTGGTTTGAATTTTGTTAGTGGGATTATTTGTAAATCTAAAGCTCTAAGGTTTGAGAGGATGCTGTTTCGTGCTACAAGGGGAAATATGCTTTTCAACCAAGCACCAGCAGATGAACATATCATGGATCCTTTATCTACTGAAATGGTTGAGCAGACAGTTTTTGTAGTCTTCTTCTCAGGGTTGCAGGCAAAAGCAAAGATTCTGAAAATTTGTGAGGCCTTTGGTGCAAATTGCTACCCTGTTCCTGAAGATATAACTAAGCAGAGGCAAATAACTAGAGAAGTTTCATCACGTCTTGCTGAATTGGAAGCAACCTTGGATGCAGGGATTCGCCACAGAAATAAGGCCCTTACTTCTGTAGGGTTTCACTTGGCAAAATGGATGAACATGGTAAGAAGGGAGAAAGCTGTATATGATACATTGAACATGTTAAACTTTGATGTTACCAAAAAATGCCTTGTTGGAGAAGGCTGGTGTCCTATATTTGCAAAAACAAAGATTCAGGAGGCGTTGGAACGTGCAACATTTGATAGCAATTCACAAGTGGGTGTAATATTTCATGTGATGGATGCAATAGAATCACCTCCTACATATTTTAGAACCAACAACTTCACAAGTGCATTTCAGGAAATTGTTGATGCATATGGTGTAGCAAGATATCAAGAAGCAAATCCTGCAGTGTACACTGTTATTACATTTCCATTTCTTTTCGCTGTGATGTTTGGGGACTGGGGTCATGGAATATGCTTGTTGATGGGAGCATTAGTTCTTATAGCCCGTGAAAGAAAGCTCAGTGCTCAGAAACTCGGGAGCTTTATGGAGATGCTATTTGGTGGGCGTTATGTACTTCTTTTAATGTCTCTGTTCTCAATTTACTGTGGGTTGATTTACAATGAGTTCTTCTCTGTCCCTTTTCACATATTTGGTGGGTCTGCTTACAAATGCAGAGATGCTTCTTGCAGTGATGCACACACTGTTGGCTTAATCAAGTACAGAGATCCATACCCATTTGGTGTGGATCCTAGTTGGCGTGGAAGTCGTTCAGAACTGCCCTTTTTGAATTCTCTCAAAATGAAATTGTCTATTTTGTTGGGTGTGGCGCAAATGAACGTAGGAATTATTCTCAGTTACTTCAATGCACGGTTTTTCAGCAGCTCAATTGATATAAGGTACCAGTTTGTGCCACAGATGATCTTCCTTAACAGTCTTTTTGGATATCTTTCACTTCTAGTTGTCATCAAGTGGTGCACCGGCTCTAAGGCTGACCTCTATCATGTAATGATTTACATGTTCTTAAGTCCAACTGATGATCTTGGGGAAAATCAGTTGTTTTGGGGCCAAAGACCACTTCAGATCATTTTGTTACTCTTGGCTCTAATTGCAGTCCCCTGGATGCTCTTTCCAAAACCTTTCATTTTGAGGAAGCGTCATACAGAGAGGTTTCAAGGTCGCACATATGGGATGCTTGGTACCTCTGAAATGGATCTTGACGTGGAAACTGATCCTGTGAGGCAACATCATGAGGAGTTTAATTTCAGTGAGGTGTTTGTGCACCAGATGATACACTCCATAGAGTTTGTTCTTGGTGCAGTCTCAAATACGGCGTCCTACCTTCGATTGTGGGCTTTGAGCTTGGCCCACTCAGAGTTGTCAACTGTGTTCTACGAGAAAGTCCTTCTCCTCGCCTGGGGGTATGACAGCTTTATCATCCGGTTAATTGGGTTAGCAGTTTTTGCCTTTGCAACTGCCTTCATATTGCTCATGATGGAGACACTCAGCGCTTTTCTTCATGCCTTGCGTCTTCATTGGGTGGAATTTCAAAATAAGTTCTACCTTGGTGATGGGTATAAGTTCAAACCTTTTTCCTTCGCCTCCATAGCTACTGAGGATGAAGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000139 GO:0000325 GO:0003674 GO:0003824 GO:0005215 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005798 GO:0005802 GO:0006139 GO:0006163 GO:0006164 GO:0006725 GO:0006753 GO:0006754 GO:0006793 GO:0006796 GO:0006807 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006885 GO:0007035 GO:0008150 GO:0008152 GO:0008324 GO:0009058 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009167 GO:0009168 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0012505 GO:0012506 GO:0012510 GO:0015075 GO:0015077 GO:0015078 GO:0015318 GO:0015399 GO:0015405 GO:0015672 GO:0015985 GO:0015986 GO:0016020 GO:0016043 GO:0016462 GO:0016469 GO:0016471 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0017144 GO:0018130 GO:0019438 GO:0019637 GO:0019693 GO:0019725 GO:0019829 GO:0019899 GO:0022607 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030003 GO:0030004 GO:0030133 GO:0030135 GO:0030136 GO:0030140 GO:0030641 GO:0030658 GO:0030659 GO:0030660 GO:0030662 GO:0030665 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032588 GO:0032991 GO:0033176 GO:0034220 GO:0034622 GO:0034641 GO:0034654 GO:0036442 GO:0042470 GO:0042592 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0043933 GO:0044085 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0044769 GO:0045851 GO:0046034 GO:0046390 GO:0046483 GO:0046961 GO:0048770 GO:0048878 GO:0050801 GO:0051117 GO:0051179 GO:0051234 GO:0051452 GO:0051453 GO:0055067 GO:0055080 GO:0055082 GO:0055085 GO:0055086 GO:0065003 GO:0065007 GO:0065008 GO:0070070 GO:0070071 GO:0070072 GO:0071704 GO:0071840 GO:0072521 GO:0072522 GO:0090407 GO:0090662 GO:0097708 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098791 GO:0098796 GO:0098805 GO:0099131 GO:0099132 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1902600
KEGG Pathways
Metabolic & Signaling
ko00190 ko01100 ko04142 ko04145 ko04721 ko04966 ko05110 ko05120 ko05152 ko05165 ko05323 map00190 map01100 map04142 map04145 map04721 map04966 map05110 map05120 map05152 map05165 map05323
Pfam Domains
Protein Families
V_ATPase_I

Protein Analysis

864

Amino Acids

98.62

Weight (kDa)

5.95

Isoelectric Point (pI)

40.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
V_ATPase_I PF01496 93 - 855 2.9e-286 V-type ATPase 116kDa subunit family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 1866, 2165
AccB1I GGYRCC 2 cut(s) 1866, 2165
AccB7I CCANNNNNTGG 1 cut(s) 231
AciI CCGC 2 cut(s) 97, 260
AcsI RAATTY 4 cut(s) 703, 903, 1756, 2510
AcuI CTGAAG 2 cut(s) 963, 2030
AcyI GRCGYC 1 cut(s) 2300
AfaI GTAC 5 cut(s) 1379, 1542, 1688, 1868, 2167
AfeI AGCGCT 1 cut(s) 2476
AfiI CCNNNNNNNGG 2 cut(s) 231, 2383
AflII CTTAAG 1 cut(s) 1990
AflIII ACRYGT 2 cut(s) 1122, 1983
AjiI CACGTC 2 cut(s) 989, 2188
AjnI CCWGG 4 cut(s) 12, 525, 2084, 2381
AjuI GAANNNNNNNTTGG 2 cut(s) 2028, 2060
AloI GAACNNNNNNTCC 2 cut(s) 1446, 1478
Alw21I GWGCWC 6 cut(s) 203, 462, 525, 1495, 1946, 2250
Alw26I GTCTC 3 cut(s) 1560, 2293, 2459
Alw44I GTGCAC 2 cut(s) 1942, 2246
Ama87I CYCGRG 1 cut(s) 1502
Aor51HI AGCGCT 1 cut(s) 2476
AoxI GGCC 5 cut(s) 124, 912, 1041, 2032, 2331
ApaLI GTGCAC 2 cut(s) 1942, 2246
ApeKI GCWGC 2 cut(s) 352, 1848
ApoI RAATTY 4 cut(s) 703, 903, 1756, 2510
ArsI GACNNNNNNTTYG 2 cut(s) 2285, 2317
Asp700I GAANNNNTTC 2 cut(s) 501, 1735
Asp718I GGTACC 2 cut(s) 1866, 2165
AspLEI GCGC 4 cut(s) 45, 357, 1798, 2477
AspS9I GGNCC 3 cut(s) 1042, 2032, 2332
AsuHPI GGTGA 7 cut(s) 86, 139, 334, 467, 653, 1269, 2546
AvaI CYCGRG 1 cut(s) 1502
BaeGI GKGCMC 2 cut(s) 1946, 2250
BamHI GGATCC 2 cut(s) 820, 1714
BanI GGYRCC 2 cut(s) 1866, 2165
BanII GRGCYC 1 cut(s) 525
BbsI GAAGAC 2 cut(s) 856, 2489
Bbv12I GWGCWC 6 cut(s) 203, 462, 525, 1495, 1946, 2250
BbvI GCAGC 2 cut(s) 339, 1860
BccI CCATC 6 cut(s) 225, 532, 1255, 1444, 2455, 2531
BceAI ACGGC 1 cut(s) 2313
BciT130I CCWGG 4 cut(s) 14, 527, 2086, 2383
BcoDI GTCTC 3 cut(s) 1560, 2293, 2459
BfaI CTAG 5 cut(s) 608, 972, 1719, 1925, 2593
BfmI CTRYAG 2 cut(s) 1053, 1371
BfoI RGCGCY 1 cut(s) 2478
BfrI CTTAAG 1 cut(s) 1990
BisI GCNGC 2 cut(s) 353, 1849
BlsI GCNGC 2 cut(s) 354, 1850
Bme1390I CCNGG 4 cut(s) 14, 527, 2086, 2383
BmeT110I CYCGRG 1 cut(s) 1502
BmgBI CACGTC 2 cut(s) 989, 2188
BmgT120I GGNCC 3 cut(s) 1042, 2032, 2332
BmiI GGNNCC 5 cut(s) 822, 1716, 1868, 2033, 2167
BmrFI CCNGG 4 cut(s) 14, 527, 2086, 2383
BmrI ACTGGG 1 cut(s) 1435
BmuI ACTGGG 1 cut(s) 1435
BoxI GACNNNNGTC 2 cut(s) 62, 1428
BpiI GAAGAC 2 cut(s) 856, 2489
BplI GAGNNNNNCTC 2 cut(s) 1583, 1615
BpmI CTGGAG 2 cut(s) 548, 680
BsaHI GRCGYC 1 cut(s) 2300
BsaJI CCNNGG 5 cut(s) 30, 1011, 2084, 2382, 2530
BsaWI WCCGGW 1 cut(s) 2406
BsaXI ACNNNNNCTCC 4 cut(s) 134, 164, 1446, 1476
Bsc4I CCNNNNNNNGG 2 cut(s) 231, 2383
Bse118I RCCGGY 1 cut(s) 1946
Bse1I ACTGG 4 cut(s) 225, 407, 1430, 1870
Bse3DI GCAATG 1 cut(s) 653
BseBI CCWGG 4 cut(s) 14, 527, 2086, 2383
BseDI CCNNGG 5 cut(s) 30, 1011, 2084, 2382, 2530
BseGI GGATG 9 cut(s) 75, 759, 1021, 1083, 1270, 2094, 2163, 2403, 2590
BseLI CCNNNNNNNGG 2 cut(s) 231, 2383
BseMI GCAATG 1 cut(s) 653
BseMII CTCAG 7 cut(s) 885, 1501, 1508, 1834, 2352, 2485, 2571
BseNI ACTGG 4 cut(s) 225, 407, 1430, 1870
BseRI GAGGAG 3 cut(s) 119, 2234, 2366
BseSI GKGCMC 2 cut(s) 1946, 2250
BseXI GCAGC 2 cut(s) 339, 1860
BsgI GTGCAG 3 cut(s) 226, 354, 2304
BshFI GGCC 5 cut(s) 126, 914, 1043, 2034, 2333
BshNI GGYRCC 2 cut(s) 1866, 2165
BsiHKAI GWGCWC 6 cut(s) 203, 462, 525, 1495, 1946, 2250
BsiHKCI CYCGRG 1 cut(s) 1502
BsiSI CCGG 2 cut(s) 1947, 2407
BslFI GGGAC 3 cut(s) 1436, 1589, 2066
BslI CCNNNNNNNGG 2 cut(s) 231, 2383
BsmAI GTCTC 3 cut(s) 1560, 2293, 2459
BsmFI GGGAC 3 cut(s) 1436, 1589, 2066
BsnI GGCC 5 cut(s) 126, 914, 1043, 2034, 2333
BsoBI CYCGRG 1 cut(s) 1502
Bsp1286I GDGCHC 6 cut(s) 203, 462, 525, 1495, 1946, 2250
Bsp1407I TGTACA 1 cut(s) 1377
Bsp19I CCATGG 1 cut(s) 30
BspACI CCGC 2 cut(s) 97, 260
BspANI GGCC 5 cut(s) 126, 914, 1043, 2034, 2333
BspCNI CTCAG 7 cut(s) 884, 1500, 1507, 1833, 2351, 2484, 2572
BspHI TCATGA 2 cut(s) 2215, 2457
BspLI GGNNCC 5 cut(s) 822, 1716, 1868, 2033, 2167
BspMAI CTGCAG 1 cut(s) 1375
BspT107I GGYRCC 2 cut(s) 1866, 2165
BspTI CTTAAG 1 cut(s) 1990
BsrDI GCAATG 1 cut(s) 653
BsrFI RCCGGY 1 cut(s) 1946
BsrGI TGTACA 1 cut(s) 1377
BsrI ACTGG 4 cut(s) 225, 407, 1430, 1870
BssAI RCCGGY 1 cut(s) 1946
BssECI CCNNGG 5 cut(s) 30, 1011, 2084, 2382, 2530
BssNI GRCGYC 1 cut(s) 2300
BssT1I CCWWGG 3 cut(s) 30, 1011, 2530
Bst2UI CCWGG 4 cut(s) 14, 527, 2086, 2383
Bst4CI ACNGT 7 cut(s) 853, 1384, 1574, 1672, 1839, 1901, 2353
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 2300
BstAFI CTTAAG 1 cut(s) 1990
BstAPI GCANNNNNTGC 2 cut(s) 1370, 2429
BstAUI TGTACA 1 cut(s) 1377
BstC8I GCNNGC 1 cut(s) 882
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 9 cut(s) 75, 759, 1021, 1083, 1270, 2094, 2163, 2403, 2590
BstH2I RGCGCY 1 cut(s) 2478
BstHHI GCGC 4 cut(s) 45, 357, 1798, 2477
BstMAI GTCTC 3 cut(s) 1560, 2293, 2459
BstMWI GCNNNNNNNGC 6 cut(s) 123, 920, 1370, 2075, 2429, 2573
BstNI CCWGG 4 cut(s) 14, 527, 2086, 2383
BstNSI RCATGY 2 cut(s) 1126, 1987
BstPAI GACNNNNGTC 2 cut(s) 62, 1428
BstSCI CCNGG 4 cut(s) 12, 525, 2084, 2381
BstSFI CTRYAG 2 cut(s) 1053, 1371
BstSLI GKGCMC 2 cut(s) 1946, 2250
BstV1I GCAGC 2 cut(s) 339, 1860
BstV2I GAAGAC 2 cut(s) 856, 2489
BstX2I RGATCY 5 cut(s) 34, 820, 1693, 1714, 2179
BstYI RGATCY 5 cut(s) 34, 820, 1693, 1714, 2179
BsuRI GGCC 5 cut(s) 126, 914, 1043, 2034, 2333
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 1 cut(s) 343
BtrI CACGTC 2 cut(s) 989, 2188
BtsCI GGATG 9 cut(s) 75, 759, 1021, 1083, 1270, 2094, 2163, 2403, 2590
BtsI GCAGTG 2 cut(s) 1380, 1663
BtsIMutI CAGTG 7 cut(s) 654, 1380, 1380, 1495, 1663, 1668, 2239
Cac8I GCNNGC 1 cut(s) 882
CciI TCATGA 2 cut(s) 2215, 2457
CfoI GCGC 4 cut(s) 45, 357, 1798, 2477
Cfr10I RCCGGY 1 cut(s) 1946
Cfr13I GGNCC 3 cut(s) 1042, 2032, 2332
CseI GACGC 3 cut(s) 2111, 2289, 2483
Csp6I GTAC 5 cut(s) 1378, 1541, 1687, 1867, 2166
CspCI CAANNNNNGTGG 2 cut(s) 193, 228
CviQI GTAC 5 cut(s) 1378, 1541, 1687, 1867, 2166
DraI TTTAAA 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Ecl136II GAGCTC 1 cut(s) 523
Eco130I CCWWGG 3 cut(s) 30, 1011, 2530
Eco147I AGGCCT 1 cut(s) 914
Eco24I GRGCYC 1 cut(s) 525
Eco32I GATATC 4 cut(s) 424, 670, 1355, 1913
Eco47III AGCGCT 1 cut(s) 2476
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 2 cut(s) 963, 2030
Eco88I CYCGRG 1 cut(s) 1502
EcoICRI GAGCTC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 1042
EcoRI GAATTC 1 cut(s) 1756
EcoRII CCWGG 4 cut(s) 12, 525, 2084, 2381
EcoRV GATATC 4 cut(s) 424, 670, 1355, 1913
EcoT14I CCWWGG 3 cut(s) 30, 1011, 2530
EcoT22I ATGCAT 1 cut(s) 1339
EcoT38I GRGCYC 1 cut(s) 525
ErhI CCWWGG 3 cut(s) 30, 1011, 2530
FalI AAGNNNNNCTT 4 cut(s) 44, 76, 341, 373
FaqI GGGAC 3 cut(s) 1436, 1589, 2066
FauNDI CATATG 2 cut(s) 1339, 2152
Fnu4HI GCNGC 2 cut(s) 353, 1849
FokI GGATG 8 cut(s) 62, 766, 1028, 1090, 1277, 2101, 2170, 2390
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 2 cut(s) 353, 1849
FspBI CTAG 5 cut(s) 608, 972, 1719, 1925, 2593
GlaI GCGC 4 cut(s) 44, 356, 1797, 2476
GluI GCNGC 2 cut(s) 353, 1849
GsuI CTGGAG 2 cut(s) 548, 680
HaeII RGCGCY 1 cut(s) 2478
HaeIII GGCC 5 cut(s) 126, 914, 1043, 2034, 2333
HapII CCGG 2 cut(s) 1947, 2407
HgaI GACGC 3 cut(s) 2111, 2289, 2483
HhaI GCGC 4 cut(s) 45, 357, 1798, 2477
Hin1I GRCGYC 1 cut(s) 2300
Hin6I GCGC 4 cut(s) 43, 355, 1796, 2475
HinP1I GCGC 4 cut(s) 43, 355, 1796, 2475
HincII GTYRAC 2 cut(s) 222, 2349
HindII GTYRAC 2 cut(s) 222, 2349
HinfI GANTC 7 cut(s) 86, 167, 644, 895, 1024, 1195, 1274
HpaII CCGG 2 cut(s) 1947, 2407
HphI GGTGA 7 cut(s) 86, 139, 334, 467, 653, 1269, 2546
Hpy166II GTNNAC 7 cut(s) 222, 622, 1378, 1380, 1944, 2248, 2349
Hpy8I GTNNAC 7 cut(s) 222, 622, 1378, 1380, 1944, 2248, 2349
Hpy99I CGWCG 1 cut(s) 86
HpyAV CCTTC 8 cut(s) 70, 182, 1086, 1157, 2318, 2381, 2455, 2572
HpyCH4III ACNGT 7 cut(s) 853, 1384, 1574, 1672, 1839, 1901, 2353
HpyCH4IV ACGT 5 cut(s) 403, 988, 1213, 1806, 2187
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 920, 1370, 2075, 2429, 2573
HpySE526I ACGT 5 cut(s) 403, 988, 1213, 1806, 2187
Hsp92I GRCGYC 1 cut(s) 2300
HspAI GCGC 4 cut(s) 43, 355, 1796, 2475
KpnI GGTACC 2 cut(s) 1870, 2169
LmnI GCTCC 5 cut(s) 50, 528, 661, 1454, 1506
Lsp1109I GCAGC 2 cut(s) 339, 1860
MaeI CTAG 5 cut(s) 608, 972, 1719, 1925, 2593
MaeII ACGT 5 cut(s) 403, 988, 1213, 1806, 2187
MaeIII GTNAC 4 cut(s) 140, 1138, 1823, 2058
MboII GAAGA 9 cut(s) 64, 623, 856, 859, 956, 1588, 1882, 2474, 2489
MfeI CAATTG 1 cut(s) 1854
MflI RGATCY 5 cut(s) 34, 820, 1693, 1714, 2179
MhlI GDGCHC 6 cut(s) 203, 462, 525, 1495, 1946, 2250
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 4 cut(s) 411, 1138, 1188, 2021
Mph1103I ATGCAT 1 cut(s) 1339
MroXI GAANNNNTTC 2 cut(s) 501, 1735
MslI CAYNNNNRTG 1 cut(s) 465
MspCI CTTAAG 1 cut(s) 1990
MspI CCGG 2 cut(s) 1947, 2407
MspR9I CCNGG 4 cut(s) 14, 527, 2086, 2383
MunI CAATTG 1 cut(s) 1854
MvaI CCWGG 4 cut(s) 14, 527, 2086, 2383
MwoI GCNNNNNNNGC 6 cut(s) 123, 920, 1370, 2075, 2429, 2573
NcoI CCATGG 1 cut(s) 30
NdeI CATATG 2 cut(s) 1339, 2152
NlaIV GGNNCC 5 cut(s) 822, 1716, 1868, 2033, 2167
NmeAIII GCCGAG 1 cut(s) 93
NmuCI GTSAC 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 1339
NspI RCATGY 2 cut(s) 1126, 1987
PagI TCATGA 2 cut(s) 2215, 2457
PceI AGGCCT 1 cut(s) 914
PciI ACATGT 2 cut(s) 1122, 1983
PdmI GAANNNNTTC 2 cut(s) 501, 1735
PfeI GAWTC 6 cut(s) 167, 644, 895, 1024, 1195, 1274
PflMI CCANNNNNTGG 1 cut(s) 231
PfoI TCCNGGA 2 cut(s) 12, 525
PkrI GCNGC 2 cut(s) 354, 1850
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PscI ACATGT 2 cut(s) 1122, 1983
PshAI GACNNNNGTC 2 cut(s) 62, 1428
Psp124BI GAGCTC 1 cut(s) 525
Psp6I CCWGG 4 cut(s) 12, 525, 2084, 2381
PspGI CCWGG 4 cut(s) 12, 525, 2084, 2381
PspN4I GGNNCC 5 cut(s) 822, 1716, 1868, 2033, 2167
PspPI GGNCC 3 cut(s) 1042, 2032, 2332
PstI CTGCAG 1 cut(s) 1375
PsuI RGATCY 5 cut(s) 34, 820, 1693, 1714, 2179
RsaI GTAC 5 cut(s) 1379, 1542, 1688, 1868, 2167
RsaNI GTAC 5 cut(s) 1378, 1541, 1687, 1867, 2166
RseI CAYNNNNRTG 1 cut(s) 465
SacI GAGCTC 1 cut(s) 525
SatI GCNGC 2 cut(s) 353, 1849
Sau96I GGNCC 3 cut(s) 1042, 2032, 2332
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 4 cut(s) 14, 527, 2086, 2383
SduI GDGCHC 6 cut(s) 203, 462, 525, 1495, 1946, 2250
SfcI CTRYAG 2 cut(s) 1053, 1371
SmiMI CAYNNNNRTG 1 cut(s) 465
SmlI CTYRAG 1 cut(s) 1990
SmoI CTYRAG 1 cut(s) 1990
SseBI AGGCCT 1 cut(s) 914
SsiI CCGC 2 cut(s) 97, 260
SspI AATATT 1 cut(s) 1251
SspMI CTAG 5 cut(s) 608, 972, 1719, 1925, 2593
SstI GAGCTC 1 cut(s) 525
StuI AGGCCT 1 cut(s) 914
StyD4I CCNGG 4 cut(s) 12, 525, 2084, 2381
StyI CCWWGG 3 cut(s) 30, 1011, 2530
TaaI ACNGT 7 cut(s) 853, 1384, 1574, 1672, 1839, 1901, 2353
TaiI ACGT 5 cut(s) 406, 991, 1216, 1809, 2190
TaqI TCGA 4 cut(s) 84, 426, 505, 2311
TatI WGTACW 3 cut(s) 1377, 1540, 1686
TfiI GAWTC 6 cut(s) 167, 644, 895, 1024, 1195, 1274
TscAI CASTG 7 cut(s) 654, 1380, 1387, 1495, 1663, 1675, 2239
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 2 cut(s) 352, 1848
Tsp45I GTSAC 1 cut(s) 140
TspGWI ACGGA 1 cut(s) 128
TspRI CASTG 7 cut(s) 654, 1380, 1387, 1495, 1663, 1675, 2239
Van91I CCANNNNNTGG 1 cut(s) 231
Vha464I CTTAAG 1 cut(s) 1990
VneI GTGCAC 2 cut(s) 1942, 2246
XapI RAATTY 4 cut(s) 703, 903, 1756, 2510
XceI RCATGY 2 cut(s) 1126, 1987
XmnI GAANNNNTTC 2 cut(s) 501, 1735
XspI CTAG 5 cut(s) 608, 972, 1719, 1925, 2593
Zsp2I ATGCAT 1 cut(s) 1339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.