RchiOBHm_Chr5g0068861

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0018617
Physical Location & Seq
Reverse (-)
74775051 .. 74777339
2289 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34426

Sequence Viewer

Length: 1098 bp
ATGTTGCTGCTTGTATCATTGATCATGATCCAGGTGGGGAACATCGATTCCAAGTGCTTTTCTGCAATATTTAATTTTGGTGACTCCACTTCGGATACGGGTGAAGACCATGCAGCCTTTCCAGACCACCTGCCGGTACCTTATGGGTCTACATTCTTCAAGAAACCTGCCGGAAGAGCTTCAGATGGCCGACTGATCATCGACTTTCTGGCTGAAGCTTTGGGACTTCACTTTTTGACCCCTTATCTGAAGCCAGAGGGATCATTCAAAAAAGGCGTCAATTTTGCCGCAGTGGGAGCCGCTGCGCTTCCTCCAAACAGCTCCTTTCCTACTTTGTTTCCTCTCAGTGTGTTCAATCTGGGTGTCCAGCTGCAACAAATGCGGGAATTCAAGTCCATTGTGGAAGAAGTCCATAAGCACGGGTCTAAGAAGAGGTTTCCTTCTCAGAAGGTCTTTGGAAAGTCTTTATACATGCTCTATATAGGCCAAGCCGATATAGTATCTCAAGTGGAAACGCTTGGTGCTGATGGAGTGAAGCAATTCTTTCCCCAAATGGTCTCCTCTATTTCTCACACTATACGGGAGCTGTATGGCTTGGGCGGGCGTACATTTTTGGTGTTCGGTGTGGGACCTGCGGGATGTGATCCTATAATTAGGGTTATAGTGAATCAGGCCGGTTCTTATCTTGATTCAGCTGGATGTTATTTTCCTTTGAATGATGCAGTTTCGGAGTACAACAAGCTGTTACGGGAGTCACTCTGGGAAATTAGAGGCGATAACCCAGAAGCATCCATCATATACGTCGACACCTTCAGCGTGAAGCTAGATCTCTTTAAGAATCCGACACACTATGGGCTCAAATATGGAGCAAAAGTTTGCTGCGGACATGGAGGAGGTCTTTACAATTTTGACCTCCATGCTCCGTGTGGGTTTCCAAATGCAACGGCTTGCAAGGACCCATGGAACTATGTTTCCTGGGATGGAACACATTTAACCGAAGCCGCAAACAGAGTGATTGCTAGGGAAATTCTGAATGGGTCACTCTTCGAACCCCCGTTTCCCTTGAAGAAGCTCTGTCGCATCCATGGTTCCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

365

Amino Acids

39.99

Weight (kDa)

7.15

Isoelectric Point (pI)

28.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 23 - 343 5.3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 138
Acc36I ACCTGC 3 cut(s) 138, 175, 640
Acc65I GGTACC 1 cut(s) 136
AccB1I GGYRCC 1 cut(s) 136
AccI GTMKAC 2 cut(s) 149, 804
AciI CCGC 7 cut(s) 288, 300, 382, 600, 635, 882, 1002
AclWI GGATC 3 cut(s) 22, 268, 638
AcoI YGGCCR 1 cut(s) 187
AcsI RAATTY 2 cut(s) 386, 1026
AcuI CTGAAG 4 cut(s) 165, 234, 269, 796
AcyI GRCGYC 1 cut(s) 276
AfaI GTAC 3 cut(s) 138, 607, 734
AfiI CCNNNNNNNGG 1 cut(s) 133
AgsI TTSAA 6 cut(s) 160, 268, 355, 391, 715, 1066
AjnI CCWGG 2 cut(s) 30, 974
AloI GAACNNNNNNTCC 4 cut(s) 32, 64, 956, 988
AluBI AGCT 9 cut(s) 179, 218, 321, 370, 586, 695, 742, 823, 1072
AluI AGCT 9 cut(s) 179, 218, 321, 370, 586, 695, 742, 823, 1072
Alw26I GTCTC 1 cut(s) 562
AlwI GGATC 3 cut(s) 22, 268, 638
AoxI GGCC 3 cut(s) 187, 484, 672
ApeKI GCWGC 5 cut(s) 7, 113, 302, 370, 879
ApoI RAATTY 2 cut(s) 386, 1026
ArsI GACNNNNNNTTYG 2 cut(s) 261, 293
Asp700I GAANNNNTTC 2 cut(s) 178, 539
Asp718I GGTACC 1 cut(s) 136
AspLEI GCGC 1 cut(s) 307
AspS9I GGNCC 2 cut(s) 629, 955
AsuHPI GGTGA 2 cut(s) 92, 113
AsuII TTCGAA 1 cut(s) 1047
AvaII GGWCC 2 cut(s) 629, 955
BanI GGYRCC 1 cut(s) 136
BanII GRGCYC 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 111
BbvI GCAGC 4 cut(s) 125, 289, 357, 866
BccI CCATC 4 cut(s) 179, 521, 800, 974
BceAI ACGGC 1 cut(s) 960
BciT130I CCWGG 2 cut(s) 32, 976
BciVI GTATCC 1 cut(s) 88
BclI TGATCA 2 cut(s) 21, 195
BcoDI GTCTC 1 cut(s) 562
BfaI CTAG 2 cut(s) 824, 1020
BfuAI ACCTGC 3 cut(s) 138, 175, 640
BfuI GTATCC 1 cut(s) 88
BglII AGATCT 1 cut(s) 826
BisI GCNGC 8 cut(s) 8, 114, 288, 300, 303, 371, 880, 1002
BlsI GCNGC 8 cut(s) 9, 115, 289, 301, 304, 372, 881, 1003
Bme1390I CCNGG 2 cut(s) 32, 976
Bme18I GGWCC 2 cut(s) 629, 955
BmgT120I GGNCC 2 cut(s) 629, 955
BmiI GGNNCC 5 cut(s) 138, 298, 630, 957, 1090
BmrFI CCNGG 2 cut(s) 32, 976
BmsI GCATC 3 cut(s) 709, 797, 1089
BpiI GAAGAC 1 cut(s) 111
Bpu14I TTCGAA 1 cut(s) 1047
BpuEI CTTGAG 1 cut(s) 489
Bsa29I ATCGAT 1 cut(s) 45
BsaBI GATNNNNATC 1 cut(s) 26
BsaHI GRCGYC 1 cut(s) 276
BsaI GGTCTC 1 cut(s) 562
BsaJI CCNNGG 3 cut(s) 959, 975, 1084
BsaXI ACNNNNNCTCC 2 cut(s) 858, 888
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse118I RCCGGY 2 cut(s) 133, 674
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 2 cut(s) 32, 976
BseCI ATCGAT 1 cut(s) 45
BseDI CCNNGG 3 cut(s) 959, 975, 1084
BseGI GGATG 5 cut(s) 644, 704, 788, 985, 1080
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 1 cut(s) 133
BseMII CTCAG 2 cut(s) 358, 458
BseRI GAGGAG 2 cut(s) 550, 906
BseXI GCAGC 4 cut(s) 125, 289, 357, 866
BshFI GGCC 3 cut(s) 189, 486, 674
BshNI GGYRCC 1 cut(s) 136
BshVI ATCGAT 1 cut(s) 45
BsiSI CCGG 3 cut(s) 134, 171, 675
BslFI GGGAC 2 cut(s) 237, 642
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 1 cut(s) 562
BsmFI GGGAC 2 cut(s) 237, 642
BsnI GGCC 3 cut(s) 189, 486, 674
Bso31I GGTCTC 1 cut(s) 562
Bsp119I TTCGAA 1 cut(s) 1047
Bsp1286I GDGCHC 1 cut(s) 858
Bsp143I GATC 6 cut(s) 21, 27, 195, 260, 643, 826
Bsp19I CCATGG 2 cut(s) 959, 1084
BspACI CCGC 7 cut(s) 288, 300, 382, 600, 635, 882, 1002
BspANI GGCC 3 cut(s) 189, 486, 674
BspCNI CTCAG 2 cut(s) 357, 457
BspDI ATCGAT 1 cut(s) 45
BspHI TCATGA 1 cut(s) 24
BspLI GGNNCC 5 cut(s) 138, 298, 630, 957, 1090
BspMI ACCTGC 3 cut(s) 138, 175, 640
BspPI GGATC 3 cut(s) 22, 268, 638
BspQI GCTCTTC 1 cut(s) 169
BspT104I TTCGAA 1 cut(s) 1047
BspT107I GGYRCC 1 cut(s) 136
BspTNI GGTCTC 1 cut(s) 562
BsrFI RCCGGY 2 cut(s) 133, 674
BssAI RCCGGY 2 cut(s) 133, 674
BssECI CCNNGG 3 cut(s) 959, 975, 1084
BssMI GATC 6 cut(s) 21, 27, 195, 260, 643, 826
BssNI GRCGYC 1 cut(s) 276
BssT1I CCWWGG 2 cut(s) 959, 1084
Bst2UI CCWGG 2 cut(s) 32, 976
Bst6I CTCTTC 3 cut(s) 169, 425, 1049
BstACI GRCGYC 1 cut(s) 276
BstAPI GCANNNNNTGC 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 602, 949
BstDEI CTNAG 3 cut(s) 344, 426, 444
BstDSI CCRYGG 2 cut(s) 959, 1084
BstF5I GGATG 5 cut(s) 644, 704, 788, 985, 1080
BstHHI GCGC 1 cut(s) 307
BstKTI GATC 6 cut(s) 24, 30, 198, 263, 646, 829
BstMAI GTCTC 1 cut(s) 562
BstMBI GATC 6 cut(s) 21, 27, 195, 260, 643, 826
BstMWI GCNNNNNNNGC 3 cut(s) 176, 296, 379
BstNI CCWGG 2 cut(s) 32, 976
BstNSI RCATGY 1 cut(s) 475
BstSCI CCNGG 2 cut(s) 30, 974
BstV1I GCAGC 4 cut(s) 125, 289, 357, 866
BstV2I GAAGAC 1 cut(s) 111
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
Bsu15I ATCGAT 1 cut(s) 45
BsuI GTATCC 1 cut(s) 88
BsuRI GGCC 3 cut(s) 189, 486, 674
BsuTUI ATCGAT 1 cut(s) 45
BtgI CCRYGG 2 cut(s) 959, 1084
BtsCI GGATG 5 cut(s) 644, 704, 788, 985, 1080
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 352
BveI ACCTGC 3 cut(s) 138, 175, 640
Cac8I GCNNGC 2 cut(s) 602, 949
CciI TCATGA 1 cut(s) 24
CfoI GCGC 1 cut(s) 307
Cfr10I RCCGGY 2 cut(s) 133, 674
Cfr13I GGNCC 2 cut(s) 629, 955
ClaI ATCGAT 1 cut(s) 45
CseI GACGC 1 cut(s) 265
Csp6I GTAC 3 cut(s) 137, 606, 733
CviAII CATG 7 cut(s) 25, 110, 472, 887, 917, 960, 1085
CviQI GTAC 3 cut(s) 137, 606, 733
DdeI CTNAG 3 cut(s) 344, 426, 444
DpnI GATC 6 cut(s) 23, 29, 197, 262, 645, 828
DpnII GATC 6 cut(s) 21, 27, 195, 260, 643, 826
EaeI YGGCCR 1 cut(s) 187
Eam1104I CTCTTC 3 cut(s) 169, 425, 1049
EarI CTCTTC 3 cut(s) 169, 425, 1049
Eco130I CCWWGG 2 cut(s) 959, 1084
Eco24I GRGCYC 1 cut(s) 858
Eco31I GGTCTC 1 cut(s) 562
Eco47I GGWCC 2 cut(s) 629, 955
Eco57I CTGAAG 4 cut(s) 165, 234, 269, 796
EcoO109I RGGNCCY 2 cut(s) 629, 955
EcoRI GAATTC 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 30, 974
EcoT14I CCWWGG 2 cut(s) 959, 1084
EcoT38I GRGCYC 1 cut(s) 858
ErhI CCWWGG 2 cut(s) 959, 1084
FaeI CATG 7 cut(s) 28, 113, 475, 890, 920, 963, 1088
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 2 cut(s) 237, 642
FatI CATG 7 cut(s) 24, 109, 471, 886, 916, 959, 1084
FauI CCCGC 3 cut(s) 375, 593, 628
FbaI TGATCA 2 cut(s) 21, 195
FblI GTMKAC 2 cut(s) 149, 804
Fnu4HI GCNGC 8 cut(s) 8, 114, 288, 300, 303, 371, 880, 1002
FokI GGATG 5 cut(s) 651, 711, 775, 992, 1067
FriOI GRGCYC 1 cut(s) 858
Fsp4HI GCNGC 8 cut(s) 8, 114, 288, 300, 303, 371, 880, 1002
FspBI CTAG 2 cut(s) 824, 1020
GlaI GCGC 1 cut(s) 306
GluI GCNGC 8 cut(s) 8, 114, 288, 300, 303, 371, 880, 1002
HaeIII GGCC 3 cut(s) 189, 486, 674
HapII CCGG 3 cut(s) 134, 171, 675
HgaI GACGC 1 cut(s) 265
HhaI GCGC 1 cut(s) 307
Hin1I GRCGYC 1 cut(s) 276
Hin1II CATG 7 cut(s) 28, 113, 475, 890, 920, 963, 1088
Hin6I GCGC 1 cut(s) 305
HinP1I GCGC 1 cut(s) 305
HincII GTYRAC 1 cut(s) 805
HindII GTYRAC 1 cut(s) 805
HindIII AAGCTT 1 cut(s) 216
HinfI GANTC 6 cut(s) 47, 83, 667, 689, 752, 838
HpaII CCGG 3 cut(s) 134, 171, 675
HphI GGTGA 2 cut(s) 92, 113
Hpy166II GTNNAC 2 cut(s) 150, 805
Hpy188I TCNGA 7 cut(s) 94, 184, 249, 447, 730, 843, 1032
Hpy188III TCNNGA 4 cut(s) 25, 122, 160, 686
Hpy8I GTNNAC 2 cut(s) 150, 805
Hpy99I CGWCG 1 cut(s) 806
HpyAV CCTTC 3 cut(s) 442, 450, 820
HpyCH4IV ACGT 1 cut(s) 801
HpyCH4V TGCA 6 cut(s) 65, 113, 373, 722, 941, 951
HpyF10VI GCNNNNNNNGC 3 cut(s) 176, 296, 379
HpyF3I CTNAG 3 cut(s) 344, 426, 444
HpySE526I ACGT 1 cut(s) 801
Hsp92I GRCGYC 1 cut(s) 276
Hsp92II CATG 7 cut(s) 28, 113, 475, 890, 920, 963, 1088
HspAI GCGC 1 cut(s) 305
KpnI GGTACC 1 cut(s) 140
Ksp22I TGATCA 2 cut(s) 21, 195
Kzo9I GATC 6 cut(s) 21, 27, 195, 260, 643, 826
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 5 cut(s) 296, 326, 583, 866, 925
Lsp1109I GCAGC 4 cut(s) 125, 289, 357, 866
LweI GCATC 3 cut(s) 709, 797, 1089
MaeI CTAG 2 cut(s) 824, 1020
MaeII ACGT 1 cut(s) 801
MaeIII GTNAC 4 cut(s) 80, 744, 753, 1038
MalI GATC 6 cut(s) 23, 29, 197, 262, 645, 828
MboI GATC 6 cut(s) 21, 27, 195, 260, 643, 826
MboII GAAGA 7 cut(s) 116, 148, 186, 416, 442, 1036, 1078
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 1 cut(s) 858
MluCI AATT 8 cut(s) 73, 280, 386, 539, 651, 765, 904, 1026
MlyI GAGTC 2 cut(s) 77, 761
MmeI TCCRAC 1 cut(s) 866
MnlI CCTC 9 cut(s) 250, 321, 351, 426, 571, 764, 884, 887, 923
MroXI GAANNNNTTC 2 cut(s) 178, 539
MseI TTAA 3 cut(s) 72, 834, 992
MspA1I CMGCKG 3 cut(s) 302, 370, 695
MspI CCGG 3 cut(s) 134, 171, 675
MspR9I CCNGG 2 cut(s) 32, 976
MvaI CCWGG 2 cut(s) 32, 976
MwoI GCNNNNNNNGC 3 cut(s) 176, 296, 379
NcoI CCATGG 2 cut(s) 959, 1084
NdeII GATC 6 cut(s) 21, 27, 195, 260, 643, 826
NlaIII CATG 7 cut(s) 28, 113, 475, 890, 920, 963, 1088
NlaIV GGNNCC 5 cut(s) 138, 298, 630, 957, 1090
NmuCI GTSAC 3 cut(s) 80, 753, 1038
NspI RCATGY 1 cut(s) 475
NspV TTCGAA 1 cut(s) 1047
PagI TCATGA 1 cut(s) 24
PaqCI CACCTGC 1 cut(s) 138
PciSI GCTCTTC 1 cut(s) 169
PdmI GAANNNNTTC 2 cut(s) 178, 539
PfeI GAWTC 4 cut(s) 47, 667, 689, 838
PkrI GCNGC 8 cut(s) 9, 115, 289, 301, 304, 372, 881, 1003
PleI GAGTC 2 cut(s) 77, 760
PpsI GAGTC 2 cut(s) 77, 760
PpuMI RGGWCCY 2 cut(s) 629, 955
Psp5II RGGWCCY 2 cut(s) 629, 955
Psp6I CCWGG 2 cut(s) 30, 974
PspGI CCWGG 2 cut(s) 30, 974
PspN4I GGNNCC 5 cut(s) 138, 298, 630, 957, 1090
PspPI GGNCC 2 cut(s) 629, 955
PspPPI RGGWCCY 2 cut(s) 629, 955
PsuI RGATCY 1 cut(s) 826
PvuII CAGCTG 2 cut(s) 370, 695
RsaI GTAC 3 cut(s) 138, 607, 734
RsaNI GTAC 3 cut(s) 137, 606, 733
SalI GTCGAC 1 cut(s) 803
SapI GCTCTTC 1 cut(s) 169
SaqAI TTAA 3 cut(s) 72, 834, 992
SatI GCNGC 8 cut(s) 8, 114, 288, 300, 303, 371, 880, 1002
Sau3AI GATC 6 cut(s) 21, 27, 195, 260, 643, 826
Sau96I GGNCC 2 cut(s) 629, 955
SchI GAGTC 2 cut(s) 77, 761
ScrFI CCNGG 2 cut(s) 32, 976
SduI GDGCHC 1 cut(s) 858
SfaNI GCATC 3 cut(s) 709, 797, 1089
SfuI TTCGAA 1 cut(s) 1047
SinI GGWCC 2 cut(s) 629, 955
SmlI CTYRAG 1 cut(s) 504
SmoI CTYRAG 1 cut(s) 504
Sse9I AATT 8 cut(s) 73, 280, 386, 539, 651, 765, 904, 1026
SsiI CCGC 7 cut(s) 288, 300, 382, 600, 635, 882, 1002
SspI AATATT 1 cut(s) 69
SspMI CTAG 2 cut(s) 824, 1020
StyD4I CCNGG 2 cut(s) 30, 974
StyI CCWWGG 2 cut(s) 959, 1084
TaiI ACGT 1 cut(s) 804
TaqI TCGA 4 cut(s) 45, 201, 804, 1047
TasI AATT 8 cut(s) 73, 280, 386, 539, 651, 765, 904, 1026
TatI WGTACW 1 cut(s) 732
TauI GCSGC 3 cut(s) 290, 302, 1004
TfiI GAWTC 4 cut(s) 47, 667, 689, 838
Tru1I TTAA 3 cut(s) 72, 834, 992
Tru9I TTAA 3 cut(s) 72, 834, 992
TscAI CASTG 2 cut(s) 297, 352
TseFI GTSAC 3 cut(s) 80, 753, 1038
TseI GCWGC 5 cut(s) 7, 113, 302, 370, 879
Tsp45I GTSAC 3 cut(s) 80, 753, 1038
TspGWI ACGGA 1 cut(s) 912
TspRI CASTG 2 cut(s) 297, 352
VpaK11BI GGWCC 2 cut(s) 629, 955
XapI RAATTY 2 cut(s) 386, 1026
XceI RCATGY 1 cut(s) 475
XcmI CCANNNNNNNNNTGG 1 cut(s) 923
XmiI GTMKAC 2 cut(s) 149, 804
XmnI GAANNNNTTC 2 cut(s) 178, 539
XspI CTAG 2 cut(s) 824, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.