Rh5DG482800

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0018617
Physical Location & Seq
Reverse (-)
76046771 .. 76048997
2227 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG482800.1

Sequence Viewer

Length: 1074 bp
ATGATCCAGGTGGGGAACATCGATTCCAAGTGCTTTTCTGCAATATTTAATTTTGGTGACTCCACTTCGGATACGGGTGAAGACCATGCAGCCTTTCCAGACCACCTGCCGGTACCTTATGGGTCTACATTCTTCAAGAAACCTGCCGGAAGAGCTTCAGATGGCCGACTGATCATCGACTTTCTGGCTGAAGCTTTGGGACTTCACTTTTTGACCCCTTATCTGAAGCCAGAGGGATCATTCAAAAACGGCGTCAATTTTGCCGCAGTGGGAGCCGCTGCGCTTCCTCCAAACAGCTCCTTTCCTACTTTGTTTCCTCTCAGTGTGTTCAATCTGGGTGTCCAGCTGCAACAAATGCGGGAATTCAAGTCCATTGTGGAAGAAGTCCATAAGCACGGGTCTAAGAAGAGGTTTCCTTCTCCGAAGGTCTTTGGAAAGTCTTTATACATGCTCTATATAGGCCAAGCCGATATAGTCTCTCAAGTGGAAACGCTTGGTGCTGATGGAGTGAAGCAATTCTTTCCTCGAATGGTCTCCTCTATTTCTCACACTATACGGGAGCTGTATGGCTTGGGCGGGCGTACATTTTGGGTGTTCGGTATGGGACCTGCGGGATGTGATCCTATAATTAGGGTTATAGTGAATCAGGCCAGTTCTTATCTTGATTCAGGCGGATGTTATTTTCCTTTGAATGATGCAGTTTCGGAGTACAACAAGCTGTTACGGGAGTCACTCTGGGAAATTAGAGGCGAAAACCCAGAAGCATCCATCATATATGTCGACACCTTCAGCGTGAAGCTAGATCTCTTCAAGAACCCGACACACTATGGGCTCAAATATGGAGCAAAAGTTTGCTGCGGACATGGAGGAGGTCTTTTCAATTTTGACCTCCATGCTCAGTGTGGGAGTCCAAATGCAACGGCTTGCAAGGACCCCTGGAACTATGTTTCCTGGGATGGAGCACATTTAACCGAAGCCGCAAACAGAGTGATTGCTAGGGAAATTCTGAATGGGTCACTCTTCGAACCCTCGTTTCCCTTGAAGAAGCTCGGTCGCATCCATGGTTCCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

39.09

Weight (kDa)

7.16

Isoelectric Point (pI)

29.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 15 - 335 8.7e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 114
Acc36I ACCTGC 3 cut(s) 114, 151, 616
Acc65I GGTACC 1 cut(s) 112
AccB1I GGYRCC 1 cut(s) 112
AccI GTMKAC 2 cut(s) 125, 780
AciI CCGC 8 cut(s) 264, 276, 358, 576, 611, 672, 858, 978
AclWI GGATC 2 cut(s) 244, 614
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 2 cut(s) 362, 1002
AcuI CTGAAG 4 cut(s) 141, 210, 245, 772
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 3 cut(s) 114, 583, 710
AfiI CCNNNNNNNGG 1 cut(s) 109
AgsI TTSAA 8 cut(s) 136, 244, 331, 367, 691, 811, 880, 1042
AjnI CCWGG 3 cut(s) 6, 935, 950
AloI GAACNNNNNNTCC 4 cut(s) 8, 40, 932, 964
AluBI AGCT 8 cut(s) 155, 194, 297, 346, 562, 718, 799, 1048
AluI AGCT 8 cut(s) 155, 194, 297, 346, 562, 718, 799, 1048
Alw21I GWGCWC 1 cut(s) 964
Alw26I GTCTC 2 cut(s) 481, 538
AlwI GGATC 2 cut(s) 244, 614
AoxI GGCC 3 cut(s) 163, 460, 648
ApeKI GCWGC 4 cut(s) 89, 278, 346, 855
ApoI RAATTY 2 cut(s) 362, 1002
ArsI GACNNNNNNTTYG 2 cut(s) 237, 269
Asp700I GAANNNNTTC 2 cut(s) 154, 515
Asp718I GGTACC 1 cut(s) 112
AspLEI GCGC 1 cut(s) 283
AspS9I GGNCC 2 cut(s) 605, 931
AsuHPI GGTGA 2 cut(s) 68, 89
AsuII TTCGAA 1 cut(s) 1023
AvaII GGWCC 2 cut(s) 605, 931
BanI GGYRCC 1 cut(s) 112
BanII GRGCYC 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 1 cut(s) 964
BbvI GCAGC 4 cut(s) 101, 265, 333, 842
BccI CCATC 4 cut(s) 155, 497, 776, 950
BceAI ACGGC 2 cut(s) 265, 936
BciT130I CCWGG 3 cut(s) 8, 937, 952
BciVI GTATCC 1 cut(s) 64
BclI TGATCA 1 cut(s) 171
BcoDI GTCTC 2 cut(s) 481, 538
BfaI CTAG 2 cut(s) 800, 996
BfuAI ACCTGC 3 cut(s) 114, 151, 616
BfuI GTATCC 1 cut(s) 64
BglII AGATCT 1 cut(s) 802
BisI GCNGC 7 cut(s) 90, 264, 276, 279, 347, 856, 978
BlsI GCNGC 7 cut(s) 91, 265, 277, 280, 348, 857, 979
Bme1390I CCNGG 3 cut(s) 8, 937, 952
Bme18I GGWCC 2 cut(s) 605, 931
BmgT120I GGNCC 2 cut(s) 605, 931
BmiI GGNNCC 5 cut(s) 114, 274, 606, 933, 1066
BmrFI CCNGG 3 cut(s) 8, 937, 952
BmsI GCATC 3 cut(s) 685, 773, 1065
BpiI GAAGAC 1 cut(s) 87
Bpu14I TTCGAA 1 cut(s) 1023
BpuEI CTTGAG 1 cut(s) 465
Bsa29I ATCGAT 1 cut(s) 21
BsaHI GRCGYC 1 cut(s) 252
BsaI GGTCTC 1 cut(s) 538
BsaJI CCNNGG 3 cut(s) 935, 951, 1060
BsaXI ACNNNNNCTCC 2 cut(s) 834, 864
Bsc4I CCNNNNNNNGG 1 cut(s) 109
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 651
BseBI CCWGG 3 cut(s) 8, 937, 952
BseCI ATCGAT 1 cut(s) 21
BseDI CCNNGG 3 cut(s) 935, 951, 1060
BseGI GGATG 5 cut(s) 620, 680, 764, 961, 1056
BseLI CCNNNNNNNGG 1 cut(s) 109
BseMII CTCAG 2 cut(s) 334, 911
BseNI ACTGG 1 cut(s) 651
BseRI GAGGAG 2 cut(s) 526, 882
BseXI GCAGC 4 cut(s) 101, 265, 333, 842
Bsh1285I CGRYCG 1 cut(s) 1054
BshFI GGCC 3 cut(s) 165, 462, 650
BshNI GGYRCC 1 cut(s) 112
BshVI ATCGAT 1 cut(s) 21
BsiEI CGRYCG 1 cut(s) 1054
BsiHKAI GWGCWC 1 cut(s) 964
BsiSI CCGG 2 cut(s) 110, 147
BslFI GGGAC 2 cut(s) 213, 618
BslI CCNNNNNNNGG 1 cut(s) 109
BsmAI GTCTC 2 cut(s) 481, 538
BsmFI GGGAC 2 cut(s) 213, 618
BsnI GGCC 3 cut(s) 165, 462, 650
Bso31I GGTCTC 1 cut(s) 538
Bsp119I TTCGAA 1 cut(s) 1023
Bsp1286I GDGCHC 2 cut(s) 834, 964
Bsp143I GATC 5 cut(s) 3, 171, 236, 619, 802
Bsp19I CCATGG 1 cut(s) 1060
BspACI CCGC 8 cut(s) 264, 276, 358, 576, 611, 672, 858, 978
BspANI GGCC 3 cut(s) 165, 462, 650
BspCNI CTCAG 2 cut(s) 333, 910
BspDI ATCGAT 1 cut(s) 21
BspLI GGNNCC 5 cut(s) 114, 274, 606, 933, 1066
BspMI ACCTGC 3 cut(s) 114, 151, 616
BspPI GGATC 2 cut(s) 244, 614
BspQI GCTCTTC 1 cut(s) 145
BspT104I TTCGAA 1 cut(s) 1023
BspT107I GGYRCC 1 cut(s) 112
BspTNI GGTCTC 1 cut(s) 538
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 1 cut(s) 651
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 3 cut(s) 935, 951, 1060
BssMI GATC 5 cut(s) 3, 171, 236, 619, 802
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 1 cut(s) 1060
Bst2UI CCWGG 3 cut(s) 8, 937, 952
Bst6I CTCTTC 4 cut(s) 145, 401, 812, 1025
BstACI GRCGYC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 355
BstBI TTCGAA 1 cut(s) 1023
BstC8I GCNNGC 2 cut(s) 578, 925
BstDEI CTNAG 3 cut(s) 320, 402, 897
BstDSI CCRYGG 1 cut(s) 1060
BstF5I GGATG 5 cut(s) 620, 680, 764, 961, 1056
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 5 cut(s) 6, 174, 239, 622, 805
BstMAI GTCTC 2 cut(s) 481, 538
BstMBI GATC 5 cut(s) 3, 171, 236, 619, 802
BstMCI CGRYCG 1 cut(s) 1054
BstMWI GCNNNNNNNGC 3 cut(s) 152, 272, 355
BstNI CCWGG 3 cut(s) 8, 937, 952
BstNSI RCATGY 1 cut(s) 451
BstSCI CCNGG 3 cut(s) 6, 935, 950
BstV1I GCAGC 4 cut(s) 101, 265, 333, 842
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 1 cut(s) 802
BstYI RGATCY 1 cut(s) 802
Bsu15I ATCGAT 1 cut(s) 21
BsuI GTATCC 1 cut(s) 64
BsuRI GGCC 3 cut(s) 165, 462, 650
BsuTUI ATCGAT 1 cut(s) 21
BtgI CCRYGG 1 cut(s) 1060
BtsCI GGATG 5 cut(s) 620, 680, 764, 961, 1056
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 3 cut(s) 273, 328, 905
BveI ACCTGC 3 cut(s) 114, 151, 616
Cac8I GCNNGC 2 cut(s) 578, 925
CfoI GCGC 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 2 cut(s) 605, 931
ClaI ATCGAT 1 cut(s) 21
CseI GACGC 1 cut(s) 241
Csp6I GTAC 3 cut(s) 113, 582, 709
CviAII CATG 5 cut(s) 86, 448, 863, 893, 1061
CviQI GTAC 3 cut(s) 113, 582, 709
DdeI CTNAG 3 cut(s) 320, 402, 897
DpnI GATC 5 cut(s) 5, 173, 238, 621, 804
DpnII GATC 5 cut(s) 3, 171, 236, 619, 802
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 4 cut(s) 145, 401, 812, 1025
EarI CTCTTC 4 cut(s) 145, 401, 812, 1025
EciI GGCGGA 1 cut(s) 687
Eco130I CCWWGG 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 834
Eco31I GGTCTC 1 cut(s) 538
Eco47I GGWCC 2 cut(s) 605, 931
Eco57I CTGAAG 4 cut(s) 141, 210, 245, 772
EcoO109I RGGNCCY 2 cut(s) 605, 931
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 3 cut(s) 6, 935, 950
EcoT14I CCWWGG 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 834
ErhI CCWWGG 1 cut(s) 1060
FaeI CATG 5 cut(s) 89, 451, 866, 896, 1064
FalI AAGNNNNNCTT 2 cut(s) 503, 535
FaqI GGGAC 2 cut(s) 213, 618
FatI CATG 5 cut(s) 85, 447, 862, 892, 1060
FauI CCCGC 3 cut(s) 351, 569, 604
FbaI TGATCA 1 cut(s) 171
FblI GTMKAC 2 cut(s) 125, 780
Fnu4HI GCNGC 7 cut(s) 90, 264, 276, 279, 347, 856, 978
FokI GGATG 5 cut(s) 627, 687, 751, 968, 1043
FriOI GRGCYC 1 cut(s) 834
Fsp4HI GCNGC 7 cut(s) 90, 264, 276, 279, 347, 856, 978
FspBI CTAG 2 cut(s) 800, 996
GlaI GCGC 1 cut(s) 282
GluI GCNGC 7 cut(s) 90, 264, 276, 279, 347, 856, 978
HaeIII GGCC 3 cut(s) 165, 462, 650
HapII CCGG 2 cut(s) 110, 147
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 5 cut(s) 89, 451, 866, 896, 1064
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 192
HinfI GANTC 6 cut(s) 23, 59, 643, 665, 728, 907
HpaII CCGG 2 cut(s) 110, 147
HphI GGTGA 2 cut(s) 68, 89
Hpy166II GTNNAC 2 cut(s) 126, 781
Hpy188I TCNGA 6 cut(s) 70, 160, 225, 423, 706, 1008
Hpy188III TCNNGA 4 cut(s) 98, 136, 662, 811
Hpy8I GTNNAC 2 cut(s) 126, 781
HpyAV CCTTC 3 cut(s) 418, 426, 796
HpyCH4V TGCA 6 cut(s) 41, 89, 349, 698, 917, 927
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 272, 355
HpyF3I CTNAG 3 cut(s) 320, 402, 897
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 5 cut(s) 89, 451, 866, 896, 1064
HspAI GCGC 1 cut(s) 281
KpnI GGTACC 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 171
Kzo9I GATC 5 cut(s) 3, 171, 236, 619, 802
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 5 cut(s) 272, 302, 559, 842, 959
Lsp1109I GCAGC 4 cut(s) 101, 265, 333, 842
LweI GCATC 3 cut(s) 685, 773, 1065
MaeI CTAG 2 cut(s) 800, 996
MaeIII GTNAC 4 cut(s) 56, 720, 729, 1014
MalI GATC 5 cut(s) 5, 173, 238, 621, 804
MboI GATC 5 cut(s) 3, 171, 236, 619, 802
MboII GAAGA 8 cut(s) 92, 124, 162, 392, 418, 799, 1012, 1054
MflI RGATCY 1 cut(s) 802
MhlI GDGCHC 2 cut(s) 834, 964
MluCI AATT 8 cut(s) 49, 256, 362, 515, 627, 741, 880, 1002
MlyI GAGTC 3 cut(s) 53, 737, 916
MroXI GAANNNNTTC 2 cut(s) 154, 515
MseI TTAA 2 cut(s) 48, 968
MspA1I CMGCKG 2 cut(s) 278, 346
MspI CCGG 2 cut(s) 110, 147
MspR9I CCNGG 3 cut(s) 8, 937, 952
MvaI CCWGG 3 cut(s) 8, 937, 952
MwoI GCNNNNNNNGC 3 cut(s) 152, 272, 355
NcoI CCATGG 1 cut(s) 1060
NdeII GATC 5 cut(s) 3, 171, 236, 619, 802
NlaIII CATG 5 cut(s) 89, 451, 866, 896, 1064
NlaIV GGNNCC 5 cut(s) 114, 274, 606, 933, 1066
NmuCI GTSAC 3 cut(s) 56, 729, 1014
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 1 cut(s) 1023
PaqCI CACCTGC 1 cut(s) 114
PciSI GCTCTTC 1 cut(s) 145
PdmI GAANNNNTTC 2 cut(s) 154, 515
PfeI GAWTC 3 cut(s) 23, 643, 665
PkrI GCNGC 7 cut(s) 91, 265, 277, 280, 348, 857, 979
PleI GAGTC 3 cut(s) 53, 736, 915
PpsI GAGTC 3 cut(s) 53, 736, 915
PpuMI RGGWCCY 2 cut(s) 605, 931
Psp5II RGGWCCY 2 cut(s) 605, 931
Psp6I CCWGG 3 cut(s) 6, 935, 950
PspGI CCWGG 3 cut(s) 6, 935, 950
PspN4I GGNNCC 5 cut(s) 114, 274, 606, 933, 1066
PspPI GGNCC 2 cut(s) 605, 931
PspPPI RGGWCCY 2 cut(s) 605, 931
PsuI RGATCY 1 cut(s) 802
PvuII CAGCTG 1 cut(s) 346
RsaI GTAC 3 cut(s) 114, 583, 710
RsaNI GTAC 3 cut(s) 113, 582, 709
SalI GTCGAC 1 cut(s) 779
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 2 cut(s) 48, 968
SatI GCNGC 7 cut(s) 90, 264, 276, 279, 347, 856, 978
Sau3AI GATC 5 cut(s) 3, 171, 236, 619, 802
Sau96I GGNCC 2 cut(s) 605, 931
SchI GAGTC 3 cut(s) 53, 737, 916
ScrFI CCNGG 3 cut(s) 8, 937, 952
SduI GDGCHC 2 cut(s) 834, 964
SfaNI GCATC 3 cut(s) 685, 773, 1065
SfuI TTCGAA 1 cut(s) 1023
SinI GGWCC 2 cut(s) 605, 931
SmlI CTYRAG 1 cut(s) 480
SmoI CTYRAG 1 cut(s) 480
Sse9I AATT 8 cut(s) 49, 256, 362, 515, 627, 741, 880, 1002
SsiI CCGC 8 cut(s) 264, 276, 358, 576, 611, 672, 858, 978
SspI AATATT 1 cut(s) 45
SspMI CTAG 2 cut(s) 800, 996
StyD4I CCNGG 3 cut(s) 6, 935, 950
StyI CCWWGG 1 cut(s) 1060
TaqI TCGA 5 cut(s) 21, 177, 526, 780, 1023
TaqII GACCGA 1 cut(s) 1040
TasI AATT 8 cut(s) 49, 256, 362, 515, 627, 741, 880, 1002
TatI WGTACW 1 cut(s) 708
TauI GCSGC 3 cut(s) 266, 278, 980
TfiI GAWTC 3 cut(s) 23, 643, 665
Tru1I TTAA 2 cut(s) 48, 968
Tru9I TTAA 2 cut(s) 48, 968
TscAI CASTG 3 cut(s) 273, 328, 905
TseFI GTSAC 3 cut(s) 56, 729, 1014
TseI GCWGC 4 cut(s) 89, 278, 346, 855
Tsp45I GTSAC 3 cut(s) 56, 729, 1014
TspRI CASTG 3 cut(s) 273, 328, 905
VpaK11BI GGWCC 2 cut(s) 605, 931
XapI RAATTY 2 cut(s) 362, 1002
XceI RCATGY 1 cut(s) 451
XcmI CCANNNNNNNNNTGG 1 cut(s) 899
XmiI GTMKAC 2 cut(s) 125, 780
XmnI GAANNNNTTC 2 cut(s) 154, 515
XspI CTAG 2 cut(s) 800, 996
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.