RLG00000036014

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0018617
Physical Location & Seq
Reverse (-)
74883247 .. 74885995
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000036014

Sequence Viewer

Length: 1122 bp
ATGAGAAGAGAAGGCAGAAGAATTAGTTTGTACACCGGGCAGTGCACACACCAGCAGATTTACAAGACCAGCTGGCACCACTCGGCTGCAACGTCCAGTGATCAAGTGGGGAACATCGATTCCAAGTGCTTTTCTGCAATATTTAATTTTGGTGACTCCGTTTCGGATACGGGTGAAGACCATGCAGCCTTTCCAGACCACCTGCCGGTACCTTATGGGTCTACATTCTTCAAGAAACCTGCCGGAAGAGCTTCAGATGGCCGACTGATCATCGACTTTCTGGCTGAAGCTTTGGGACTTCATTTTTTGACCCCTTATCTGAAGCCAGAGGGATCATTCAAACACGGCGTCAATTTTGCCGCAGTGGGAGCCGCTGCGCTTCCTCCAAACAGCTCCTTTCCTACTTCGTTTCCTCTCAGTGTGTTCAATCTCGGTGTCCAGCTGCAACAAATGCGGGAATTCAAGTCCATTGTGGAAGAAGTCCATAAGCACGGGTCTAAGAAGAGGTTTCCTTCTCCGAAGGTCTTTGGAAAGTCTTTATACATGCTCTATATAGGCCAAGCCGATATAGTCTCTCAAGTGGAAACGCTTGGTGCTGATGGAGTGAAGCAATTCTTTCCCCGAATAGTCTCCTCTATTTCTCACACTATACGGGAGCTGTATGCCTTGGGCGGGCGTACATTTTGGGTGTTCGGTGTGGGACCTGCGGGATGTGATCCTATAATCAGGGTTATAGTGAATCAGGCCATTTCGGAGTACAACAAGCTGTTACGGGAGTCACTCTGGGAAATTAGAGGCGAAAACCCAGAAGCATCCATTATATATGTCGACACCTTCAGCATGAAGCTAGATCTCTTCAAGAATCCGACACGCTATGGGCTCAAATATGGAGCAAAAGTTTGCTGCGGACATGGAGGAGGTCTTTTCAATTTTGACCTCCATGCTCAGTGTGGGAGTCCAAATGCAACGGCTTGCAAGGACCCCTGGAACTATGTTTCCTGGGATGGAGCACATTTAACCGAAGCGGCAAACAGAGTGATGGCTAGGGAAATTCTGAATGGGTCACTCTTCGAACCCTCGTTTCCCTTGAAGAAGCTCGGTCGCATCCATGGTTCCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

374

Amino Acids

41.18

Weight (kDa)

8.84

Isoelectric Point (pI)

30.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 47 - 351 1.7e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 210
Acc36I ACCTGC 3 cut(s) 210, 247, 712
Acc65I GGTACC 1 cut(s) 208
AccB1I GGYRCC 2 cut(s) 75, 208
AccI GTMKAC 2 cut(s) 221, 828
AciI CCGC 7 cut(s) 360, 372, 454, 672, 707, 906, 1025
AclWI GGATC 2 cut(s) 340, 710
AcoI YGGCCR 1 cut(s) 259
AcsI RAATTY 2 cut(s) 458, 1050
AcuI CTGAAG 4 cut(s) 237, 306, 341, 820
AcyI GRCGYC 1 cut(s) 348
AfaI GTAC 4 cut(s) 32, 210, 679, 758
AfiI CCNNNNNNNGG 2 cut(s) 205, 672
AgsI TTSAA 7 cut(s) 232, 340, 427, 463, 859, 928, 1090
AjnI CCWGG 2 cut(s) 983, 998
AloI GAACNNNNNNTCC 4 cut(s) 104, 136, 980, 1012
AluBI AGCT 9 cut(s) 72, 251, 290, 393, 442, 658, 766, 847, 1096
AluI AGCT 9 cut(s) 72, 251, 290, 393, 442, 658, 766, 847, 1096
Alw21I GWGCWC 2 cut(s) 47, 1012
Alw26I GTCTC 2 cut(s) 577, 634
Alw44I GTGCAC 1 cut(s) 43
AlwI GGATC 2 cut(s) 340, 710
AoxI GGCC 3 cut(s) 259, 556, 744
ApaLI GTGCAC 1 cut(s) 43
ApeKI GCWGC 5 cut(s) 86, 185, 374, 442, 903
ApoI RAATTY 2 cut(s) 458, 1050
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
Asp700I GAANNNNTTC 2 cut(s) 250, 611
Asp718I GGTACC 1 cut(s) 208
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 2 cut(s) 701, 979
AsuC2I CCSGG 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 164, 185
AsuII TTCGAA 1 cut(s) 1071
AvaII GGWCC 2 cut(s) 701, 979
BaeGI GKGCMC 1 cut(s) 47
BanI GGYRCC 2 cut(s) 75, 208
BanII GRGCYC 1 cut(s) 882
BbsI GAAGAC 1 cut(s) 183
Bbv12I GWGCWC 2 cut(s) 47, 1012
BbvI GCAGC 5 cut(s) 73, 197, 361, 429, 890
BccI CCATC 4 cut(s) 251, 593, 998, 1033
BceAI ACGGC 2 cut(s) 361, 984
BciT130I CCWGG 2 cut(s) 985, 1000
BciVI GTATCC 1 cut(s) 160
BclI TGATCA 2 cut(s) 100, 267
BcnI CCSGG 1 cut(s) 37
BcoDI GTCTC 2 cut(s) 577, 634
BfaI CTAG 2 cut(s) 848, 1044
BfuAI ACCTGC 3 cut(s) 210, 247, 712
BfuI GTATCC 1 cut(s) 160
BglII AGATCT 1 cut(s) 850
BisI GCNGC 8 cut(s) 87, 186, 360, 372, 375, 443, 904, 1026
BlsI GCNGC 8 cut(s) 88, 187, 361, 373, 376, 444, 905, 1027
Bme1390I CCNGG 3 cut(s) 37, 985, 1000
Bme18I GGWCC 2 cut(s) 701, 979
BmgT120I GGNCC 2 cut(s) 701, 979
BmiI GGNNCC 6 cut(s) 77, 210, 370, 702, 981, 1114
BmrFI CCNGG 3 cut(s) 37, 985, 1000
BmsI GCATC 2 cut(s) 821, 1113
BpiI GAAGAC 1 cut(s) 183
Bpu14I TTCGAA 1 cut(s) 1071
BpuEI CTTGAG 1 cut(s) 561
BpuMI CCSGG 1 cut(s) 37
Bsa29I ATCGAT 1 cut(s) 117
BsaHI GRCGYC 1 cut(s) 348
BsaJI CCNNGG 4 cut(s) 666, 983, 999, 1108
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 672
Bse118I RCCGGY 1 cut(s) 205
Bse1I ACTGG 1 cut(s) 96
BseBI CCWGG 2 cut(s) 985, 1000
BseCI ATCGAT 1 cut(s) 117
BseDI CCNNGG 4 cut(s) 666, 983, 999, 1108
BseGI GGATG 4 cut(s) 716, 812, 1009, 1104
BseLI CCNNNNNNNGG 2 cut(s) 205, 672
BseMII CTCAG 2 cut(s) 430, 959
BseNI ACTGG 1 cut(s) 96
BseRI GAGGAG 2 cut(s) 622, 930
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 5 cut(s) 73, 197, 361, 429, 890
Bsh1285I CGRYCG 1 cut(s) 1102
BshFI GGCC 3 cut(s) 261, 558, 746
BshNI GGYRCC 2 cut(s) 75, 208
BshVI ATCGAT 1 cut(s) 117
BsiEI CGRYCG 1 cut(s) 1102
BsiHKAI GWGCWC 2 cut(s) 47, 1012
BsiSI CCGG 3 cut(s) 36, 206, 243
BslFI GGGAC 2 cut(s) 309, 714
BslI CCNNNNNNNGG 2 cut(s) 205, 672
BsmAI GTCTC 2 cut(s) 577, 634
BsmFI GGGAC 2 cut(s) 309, 714
BsnI GGCC 3 cut(s) 261, 558, 746
Bsp119I TTCGAA 1 cut(s) 1071
Bsp1286I GDGCHC 3 cut(s) 47, 882, 1012
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 5 cut(s) 100, 267, 332, 715, 850
Bsp19I CCATGG 1 cut(s) 1108
BspACI CCGC 7 cut(s) 360, 372, 454, 672, 707, 906, 1025
BspANI GGCC 3 cut(s) 261, 558, 746
BspCNI CTCAG 2 cut(s) 429, 958
BspDI ATCGAT 1 cut(s) 117
BspLI GGNNCC 6 cut(s) 77, 210, 370, 702, 981, 1114
BspMI ACCTGC 3 cut(s) 210, 247, 712
BspPI GGATC 2 cut(s) 340, 710
BspQI GCTCTTC 1 cut(s) 241
BspT104I TTCGAA 1 cut(s) 1071
BspT107I GGYRCC 2 cut(s) 75, 208
BsrFI RCCGGY 1 cut(s) 205
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 1 cut(s) 96
BssAI RCCGGY 1 cut(s) 205
BssECI CCNNGG 4 cut(s) 666, 983, 999, 1108
BssMI GATC 5 cut(s) 100, 267, 332, 715, 850
BssNI GRCGYC 1 cut(s) 348
BssT1I CCWWGG 2 cut(s) 666, 1108
Bst2UI CCWGG 2 cut(s) 985, 1000
Bst6I CTCTTC 4 cut(s) 241, 497, 860, 1073
BstACI GRCGYC 1 cut(s) 348
BstAPI GCANNNNNTGC 1 cut(s) 451
BstAUI TGTACA 1 cut(s) 30
BstBI TTCGAA 1 cut(s) 1071
BstC8I GCNNGC 3 cut(s) 74, 674, 973
BstDEI CTNAG 3 cut(s) 416, 498, 945
BstDSI CCRYGG 1 cut(s) 1108
BstF5I GGATG 4 cut(s) 716, 812, 1009, 1104
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 5 cut(s) 103, 270, 335, 718, 853
BstMAI GTCTC 2 cut(s) 577, 634
BstMBI GATC 5 cut(s) 100, 267, 332, 715, 850
BstMCI CGRYCG 1 cut(s) 1102
BstMWI GCNNNNNNNGC 3 cut(s) 248, 368, 451
BstNI CCWGG 2 cut(s) 985, 1000
BstNSI RCATGY 1 cut(s) 547
BstSCI CCNGG 3 cut(s) 35, 983, 998
BstSLI GKGCMC 1 cut(s) 47
BstV1I GCAGC 5 cut(s) 73, 197, 361, 429, 890
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
Bsu15I ATCGAT 1 cut(s) 117
BsuI GTATCC 1 cut(s) 160
BsuRI GGCC 3 cut(s) 261, 558, 746
BsuTUI ATCGAT 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 1108
BtsCI GGATG 4 cut(s) 716, 812, 1009, 1104
BtsI GCAGTG 2 cut(s) 47, 369
BtsIMutI CAGTG 5 cut(s) 47, 103, 369, 424, 953
BveI ACCTGC 3 cut(s) 210, 247, 712
Cac8I GCNNGC 3 cut(s) 74, 674, 973
CfoI GCGC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 205
Cfr13I GGNCC 2 cut(s) 701, 979
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 1 cut(s) 337
Csp6I GTAC 4 cut(s) 31, 209, 678, 757
CviAII CATG 6 cut(s) 182, 544, 841, 911, 941, 1109
CviQI GTAC 4 cut(s) 31, 209, 678, 757
DdeI CTNAG 3 cut(s) 416, 498, 945
DpnI GATC 5 cut(s) 102, 269, 334, 717, 852
DpnII GATC 5 cut(s) 100, 267, 332, 715, 850
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 4 cut(s) 241, 497, 860, 1073
EarI CTCTTC 4 cut(s) 241, 497, 860, 1073
Eco130I CCWWGG 2 cut(s) 666, 1108
Eco24I GRGCYC 1 cut(s) 882
Eco47I GGWCC 2 cut(s) 701, 979
Eco57I CTGAAG 4 cut(s) 237, 306, 341, 820
EcoO109I RGGNCCY 2 cut(s) 701, 979
EcoRI GAATTC 1 cut(s) 458
EcoRII CCWGG 2 cut(s) 983, 998
EcoT14I CCWWGG 2 cut(s) 666, 1108
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 2 cut(s) 666, 1108
FaeI CATG 6 cut(s) 185, 547, 844, 914, 944, 1112
FalI AAGNNNNNCTT 2 cut(s) 599, 631
FaqI GGGAC 2 cut(s) 309, 714
FatI CATG 6 cut(s) 181, 543, 840, 910, 940, 1108
FauI CCCGC 3 cut(s) 447, 665, 700
FbaI TGATCA 2 cut(s) 100, 267
FblI GTMKAC 2 cut(s) 221, 828
Fnu4HI GCNGC 8 cut(s) 87, 186, 360, 372, 375, 443, 904, 1026
FokI GGATG 4 cut(s) 723, 799, 1016, 1091
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 8 cut(s) 87, 186, 360, 372, 375, 443, 904, 1026
FspBI CTAG 2 cut(s) 848, 1044
GlaI GCGC 1 cut(s) 378
GluI GCNGC 8 cut(s) 87, 186, 360, 372, 375, 443, 904, 1026
HaeIII GGCC 3 cut(s) 261, 558, 746
HapII CCGG 3 cut(s) 36, 206, 243
HgaI GACGC 1 cut(s) 337
HhaI GCGC 1 cut(s) 379
Hin1I GRCGYC 1 cut(s) 348
Hin1II CATG 6 cut(s) 185, 547, 844, 914, 944, 1112
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HincII GTYRAC 1 cut(s) 829
HindII GTYRAC 1 cut(s) 829
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 6 cut(s) 119, 155, 739, 776, 862, 955
HpaII CCGG 3 cut(s) 36, 206, 243
HphI GGTGA 2 cut(s) 164, 185
Hpy166II GTNNAC 4 cut(s) 33, 45, 222, 829
Hpy188I TCNGA 7 cut(s) 166, 256, 321, 519, 754, 867, 1056
Hpy188III TCNNGA 3 cut(s) 194, 232, 859
Hpy8I GTNNAC 4 cut(s) 33, 45, 222, 829
HpyAV CCTTC 4 cut(s) 5, 514, 522, 844
HpyCH4IV ACGT 1 cut(s) 92
HpyCH4V TGCA 7 cut(s) 45, 89, 137, 185, 445, 965, 975
HpyF10VI GCNNNNNNNGC 3 cut(s) 248, 368, 451
HpyF3I CTNAG 3 cut(s) 416, 498, 945
HpySE526I ACGT 1 cut(s) 92
Hsp92I GRCGYC 1 cut(s) 348
Hsp92II CATG 6 cut(s) 185, 547, 844, 914, 944, 1112
HspAI GCGC 1 cut(s) 377
KpnI GGTACC 1 cut(s) 212
Ksp22I TGATCA 2 cut(s) 100, 267
Kzo9I GATC 5 cut(s) 100, 267, 332, 715, 850
LguI GCTCTTC 1 cut(s) 241
LmnI GCTCC 5 cut(s) 368, 398, 655, 890, 1007
Lsp1109I GCAGC 5 cut(s) 73, 197, 361, 429, 890
LweI GCATC 2 cut(s) 821, 1113
MaeI CTAG 2 cut(s) 848, 1044
MaeII ACGT 1 cut(s) 92
MaeIII GTNAC 4 cut(s) 152, 768, 777, 1062
MalI GATC 5 cut(s) 102, 269, 334, 717, 852
MboI GATC 5 cut(s) 100, 267, 332, 715, 850
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 3 cut(s) 47, 882, 1012
MluCI AATT 8 cut(s) 21, 145, 352, 458, 611, 789, 928, 1050
MlyI GAGTC 3 cut(s) 149, 785, 964
MmeI TCCRAC 1 cut(s) 890
MroXI GAANNNNTTC 2 cut(s) 250, 611
MseI TTAA 2 cut(s) 144, 1016
MspA1I CMGCKG 3 cut(s) 72, 374, 442
MspI CCGG 3 cut(s) 36, 206, 243
MspR9I CCNGG 3 cut(s) 37, 985, 1000
MvaI CCWGG 2 cut(s) 985, 1000
MwoI GCNNNNNNNGC 3 cut(s) 248, 368, 451
NciI CCSGG 1 cut(s) 37
NcoI CCATGG 1 cut(s) 1108
NdeII GATC 5 cut(s) 100, 267, 332, 715, 850
NlaIII CATG 6 cut(s) 185, 547, 844, 914, 944, 1112
NlaIV GGNNCC 6 cut(s) 77, 210, 370, 702, 981, 1114
NmeAIII GCCGAG 1 cut(s) 62
NmuCI GTSAC 3 cut(s) 152, 777, 1062
NspI RCATGY 1 cut(s) 547
NspV TTCGAA 1 cut(s) 1071
PaqCI CACCTGC 1 cut(s) 210
PciSI GCTCTTC 1 cut(s) 241
PcsI WCGNNNNNNNCGW 1 cut(s) 89
PdmI GAANNNNTTC 2 cut(s) 250, 611
PfeI GAWTC 3 cut(s) 119, 739, 862
PkrI GCNGC 8 cut(s) 88, 187, 361, 373, 376, 444, 905, 1027
PleI GAGTC 3 cut(s) 149, 784, 963
PpsI GAGTC 3 cut(s) 149, 784, 963
PpuMI RGGWCCY 2 cut(s) 701, 979
Psp5II RGGWCCY 2 cut(s) 701, 979
Psp6I CCWGG 2 cut(s) 983, 998
PspGI CCWGG 2 cut(s) 983, 998
PspN4I GGNNCC 6 cut(s) 77, 210, 370, 702, 981, 1114
PspPI GGNCC 2 cut(s) 701, 979
PspPPI RGGWCCY 2 cut(s) 701, 979
PsuI RGATCY 1 cut(s) 850
PvuII CAGCTG 2 cut(s) 72, 442
RsaI GTAC 4 cut(s) 32, 210, 679, 758
RsaNI GTAC 4 cut(s) 31, 209, 678, 757
SalI GTCGAC 1 cut(s) 827
SapI GCTCTTC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 144, 1016
SatI GCNGC 8 cut(s) 87, 186, 360, 372, 375, 443, 904, 1026
Sau3AI GATC 5 cut(s) 100, 267, 332, 715, 850
Sau96I GGNCC 2 cut(s) 701, 979
SchI GAGTC 3 cut(s) 149, 785, 964
ScrFI CCNGG 3 cut(s) 37, 985, 1000
SduI GDGCHC 3 cut(s) 47, 882, 1012
SfaNI GCATC 2 cut(s) 821, 1113
SfuI TTCGAA 1 cut(s) 1071
SinI GGWCC 2 cut(s) 701, 979
SmlI CTYRAG 1 cut(s) 576
SmoI CTYRAG 1 cut(s) 576
Sse9I AATT 8 cut(s) 21, 145, 352, 458, 611, 789, 928, 1050
SsiI CCGC 7 cut(s) 360, 372, 454, 672, 707, 906, 1025
SspI AATATT 1 cut(s) 141
SspMI CTAG 2 cut(s) 848, 1044
StyD4I CCNGG 3 cut(s) 35, 983, 998
StyI CCWWGG 2 cut(s) 666, 1108
TaiI ACGT 1 cut(s) 95
TaqI TCGA 4 cut(s) 117, 273, 828, 1071
TaqII GACCGA 1 cut(s) 1088
TasI AATT 8 cut(s) 21, 145, 352, 458, 611, 789, 928, 1050
TatI WGTACW 2 cut(s) 30, 756
TauI GCSGC 3 cut(s) 362, 374, 1028
TfiI GAWTC 3 cut(s) 119, 739, 862
Tru1I TTAA 2 cut(s) 144, 1016
Tru9I TTAA 2 cut(s) 144, 1016
TscAI CASTG 5 cut(s) 47, 103, 369, 424, 953
TseFI GTSAC 3 cut(s) 152, 777, 1062
TseI GCWGC 5 cut(s) 86, 185, 374, 442, 903
Tsp45I GTSAC 3 cut(s) 152, 777, 1062
TspDTI ATGAA 2 cut(s) 290, 857
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 5 cut(s) 47, 103, 369, 424, 953
VneI GTGCAC 1 cut(s) 43
VpaK11BI GGWCC 2 cut(s) 701, 979
XapI RAATTY 2 cut(s) 458, 1050
XceI RCATGY 1 cut(s) 547
XcmI CCANNNNNNNNNTGG 2 cut(s) 103, 947
XmiI GTMKAC 2 cut(s) 221, 828
XmnI GAANNNNTTC 2 cut(s) 250, 611
XspI CTAG 2 cut(s) 848, 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.