Rmu_sc0006027.1_g000012

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006027.1
OG0018617
Physical Location & Seq
Forward (+)
37436 .. 39077
1642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006027.1_g000012.1.cds

Sequence Viewer

Length: 912 bp
atgatgttgatgaacaacttggcagctgaagctttgggacttcactttttgaccccttttctgaagccagagggatcattcaaaaacggcgtcaattttgccgcagtgggagccgctgcgcttcctccaaacagctcctttcctactttgtttcctctcagtgtgttcaatctgggtgtccagctgcaacaaatgcgggaattcaagtccattgtggaagacgtccataagcacgggtctaagaagaggtttccttctccgaaggtctttggaaagtctttatacatgctctatataggccaagccgatatagtctctcaagtggaaacgcttggtgctgatggagtgaagcaattctttcctcgaatggtctcctctatttctcacactatacgggagctgtatggcttgggcgggcgtacattttgggtgttcggtatgggacctgcgggatgtgatcctataattagggttatagtgaatcaggccagttcttatcttgattcaggcggatgttattttcctttgaatgatgcagtttcggagtacaacaagctgttacgggagtcactctgggaaattagaggcgaacacccagaagcatccatcatatatgtcgacagcttcagcgtgaagctagatctcttcaagaacccgacacactatgggctcaaatatggagcaaaagtttgctgcggacatggaggaggtctttacaattttgacctccatgctcagtgtgggtgtccaaatgcaacggcttgcaaggacccctggaactatgtttcctgggatggagcacatttaaccgaagccgcaaacagagtgattgctagggaaattctgaatgggtcactcttcgaacccccgtttcccttgaagaagctctgtcgcatccatggctccacctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

303

Amino Acids

33.37

Weight (kDa)

8.35

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
Acc36I ACCTGC 1 cut(s) 454
AccI GTMKAC 1 cut(s) 618
AciI CCGC 8 cut(s) 102, 114, 196, 414, 449, 510, 696, 816
AclWI GGATC 2 cut(s) 82, 452
AcsI RAATTY 2 cut(s) 200, 840
AcuI CTGAAG 3 cut(s) 48, 83, 610
AcyI GRCGYC 2 cut(s) 90, 222
AfaI GTAC 2 cut(s) 421, 548
AgsI TTSAA 6 cut(s) 82, 169, 205, 529, 649, 880
AjnI CCWGG 2 cut(s) 773, 788
AloI GAACNNNNNNTCC 2 cut(s) 770, 802
AluBI AGCT 9 cut(s) 26, 32, 135, 184, 400, 556, 624, 637, 886
AluI AGCT 9 cut(s) 26, 32, 135, 184, 400, 556, 624, 637, 886
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 2 cut(s) 319, 376
AlwI GGATC 2 cut(s) 82, 452
AoxI GGCC 2 cut(s) 298, 486
ApeKI GCWGC 4 cut(s) 23, 116, 184, 693
ApoI RAATTY 2 cut(s) 200, 840
ArsI GACNNNNNNTTYG 2 cut(s) 75, 107
Asp700I GAANNNNTTC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 2 cut(s) 443, 769
AsuII TTCGAA 1 cut(s) 861
AvaII GGWCC 2 cut(s) 443, 769
BanII GRGCYC 1 cut(s) 672
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 802
BbvI GCAGC 4 cut(s) 35, 103, 171, 680
BccI CCATC 3 cut(s) 335, 614, 788
BceAI ACGGC 2 cut(s) 103, 774
BciT130I CCWGG 2 cut(s) 775, 790
BcoDI GTCTC 2 cut(s) 319, 376
BfaI CTAG 2 cut(s) 638, 834
BfuAI ACCTGC 1 cut(s) 454
BglII AGATCT 1 cut(s) 640
BisI GCNGC 7 cut(s) 24, 102, 114, 117, 185, 694, 816
BlsI GCNGC 7 cut(s) 25, 103, 115, 118, 186, 695, 817
Bme1390I CCNGG 2 cut(s) 775, 790
Bme18I GGWCC 2 cut(s) 443, 769
BmgT120I GGNCC 2 cut(s) 443, 769
BmiI GGNNCC 4 cut(s) 112, 444, 771, 904
BmrFI CCNGG 2 cut(s) 775, 790
BmsI GCATC 3 cut(s) 523, 611, 903
BpiI GAAGAC 1 cut(s) 225
Bpu14I TTCGAA 1 cut(s) 861
BpuEI CTTGAG 1 cut(s) 303
BsaHI GRCGYC 2 cut(s) 90, 222
BsaI GGTCTC 1 cut(s) 376
BsaJI CCNNGG 3 cut(s) 773, 789, 898
BsaXI ACNNNNNCTCC 2 cut(s) 672, 702
Bse1I ACTGG 1 cut(s) 489
BseBI CCWGG 2 cut(s) 775, 790
BseDI CCNNGG 3 cut(s) 773, 789, 898
BseGI GGATG 5 cut(s) 458, 518, 602, 799, 894
BseMII CTCAG 2 cut(s) 172, 749
BseNI ACTGG 1 cut(s) 489
BseRI GAGGAG 2 cut(s) 364, 720
BseXI GCAGC 4 cut(s) 35, 103, 171, 680
BshFI GGCC 2 cut(s) 300, 488
BsiHKAI GWGCWC 1 cut(s) 802
BslFI GGGAC 2 cut(s) 51, 456
BsmAI GTCTC 2 cut(s) 319, 376
BsmFI GGGAC 2 cut(s) 51, 456
BsnI GGCC 2 cut(s) 300, 488
Bso31I GGTCTC 1 cut(s) 376
Bsp119I TTCGAA 1 cut(s) 861
Bsp1286I GDGCHC 2 cut(s) 672, 802
Bsp143I GATC 3 cut(s) 74, 457, 640
Bsp19I CCATGG 1 cut(s) 898
BspACI CCGC 8 cut(s) 102, 114, 196, 414, 449, 510, 696, 816
BspANI GGCC 2 cut(s) 300, 488
BspCNI CTCAG 2 cut(s) 171, 748
BspLI GGNNCC 4 cut(s) 112, 444, 771, 904
BspMI ACCTGC 1 cut(s) 454
BspPI GGATC 2 cut(s) 82, 452
BspT104I TTCGAA 1 cut(s) 861
BspTNI GGTCTC 1 cut(s) 376
BsrI ACTGG 1 cut(s) 489
BssECI CCNNGG 3 cut(s) 773, 789, 898
BssMI GATC 3 cut(s) 74, 457, 640
BssNI GRCGYC 2 cut(s) 90, 222
BssT1I CCWWGG 1 cut(s) 898
Bst2UI CCWGG 2 cut(s) 775, 790
Bst6I CTCTTC 3 cut(s) 239, 650, 863
BstACI GRCGYC 2 cut(s) 90, 222
BstAPI GCANNNNNTGC 1 cut(s) 193
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 2 cut(s) 416, 763
BstDEI CTNAG 3 cut(s) 158, 240, 735
BstDSI CCRYGG 1 cut(s) 898
BstF5I GGATG 5 cut(s) 458, 518, 602, 799, 894
BstHHI GCGC 1 cut(s) 121
BstKTI GATC 3 cut(s) 77, 460, 643
BstMAI GTCTC 2 cut(s) 319, 376
BstMBI GATC 3 cut(s) 74, 457, 640
BstMWI GCNNNNNNNGC 4 cut(s) 29, 110, 193, 900
BstNI CCWGG 2 cut(s) 775, 790
BstNSI RCATGY 1 cut(s) 289
BstSCI CCNGG 2 cut(s) 773, 788
BstV1I GCAGC 4 cut(s) 35, 103, 171, 680
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 1 cut(s) 640
BstYI RGATCY 1 cut(s) 640
BsuRI GGCC 2 cut(s) 300, 488
BtgI CCRYGG 1 cut(s) 898
BtsCI GGATG 5 cut(s) 458, 518, 602, 799, 894
BtsI GCAGTG 1 cut(s) 111
BtsIMutI CAGTG 3 cut(s) 111, 166, 743
BveI ACCTGC 1 cut(s) 454
Cac8I GCNNGC 2 cut(s) 416, 763
CfoI GCGC 1 cut(s) 121
Cfr13I GGNCC 2 cut(s) 443, 769
CseI GACGC 1 cut(s) 79
Csp6I GTAC 2 cut(s) 420, 547
CviAII CATG 4 cut(s) 286, 701, 731, 899
CviQI GTAC 2 cut(s) 420, 547
DdeI CTNAG 3 cut(s) 158, 240, 735
DpnI GATC 3 cut(s) 76, 459, 642
DpnII GATC 3 cut(s) 74, 457, 640
Eam1104I CTCTTC 3 cut(s) 239, 650, 863
EarI CTCTTC 3 cut(s) 239, 650, 863
EciI GGCGGA 1 cut(s) 525
Eco130I CCWWGG 1 cut(s) 898
Eco24I GRGCYC 1 cut(s) 672
Eco31I GGTCTC 1 cut(s) 376
Eco47I GGWCC 2 cut(s) 443, 769
Eco57I CTGAAG 3 cut(s) 48, 83, 610
EcoO109I RGGNCCY 2 cut(s) 443, 769
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 2 cut(s) 773, 788
EcoT14I CCWWGG 1 cut(s) 898
EcoT38I GRGCYC 1 cut(s) 672
ErhI CCWWGG 1 cut(s) 898
FaeI CATG 4 cut(s) 289, 704, 734, 902
FalI AAGNNNNNCTT 2 cut(s) 341, 373
FaqI GGGAC 2 cut(s) 51, 456
FatI CATG 4 cut(s) 285, 700, 730, 898
FauI CCCGC 3 cut(s) 189, 407, 442
FblI GTMKAC 1 cut(s) 618
Fnu4HI GCNGC 7 cut(s) 24, 102, 114, 117, 185, 694, 816
FokI GGATG 5 cut(s) 465, 525, 589, 806, 881
FriOI GRGCYC 1 cut(s) 672
Fsp4HI GCNGC 7 cut(s) 24, 102, 114, 117, 185, 694, 816
FspBI CTAG 2 cut(s) 638, 834
GlaI GCGC 1 cut(s) 120
GluI GCNGC 7 cut(s) 24, 102, 114, 117, 185, 694, 816
HaeIII GGCC 2 cut(s) 300, 488
HgaI GACGC 1 cut(s) 79
HhaI GCGC 1 cut(s) 121
Hin1I GRCGYC 2 cut(s) 90, 222
Hin1II CATG 4 cut(s) 289, 704, 734, 902
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HincII GTYRAC 1 cut(s) 619
HindII GTYRAC 1 cut(s) 619
HindIII AAGCTT 1 cut(s) 30
HinfI GANTC 3 cut(s) 481, 503, 566
Hpy166II GTNNAC 1 cut(s) 619
Hpy188I TCNGA 4 cut(s) 63, 261, 544, 846
Hpy188III TCNNGA 2 cut(s) 500, 649
Hpy8I GTNNAC 1 cut(s) 619
HpyAV CCTTC 2 cut(s) 256, 264
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 4 cut(s) 187, 536, 755, 765
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 110, 193, 900
HpyF3I CTNAG 3 cut(s) 158, 240, 735
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 2 cut(s) 90, 222
Hsp92II CATG 4 cut(s) 289, 704, 734, 902
HspAI GCGC 1 cut(s) 119
Kzo9I GATC 3 cut(s) 74, 457, 640
LmnI GCTCC 6 cut(s) 110, 140, 397, 680, 797, 908
Lsp1109I GCAGC 4 cut(s) 35, 103, 171, 680
LweI GCATC 3 cut(s) 523, 611, 903
MaeI CTAG 2 cut(s) 638, 834
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 3 cut(s) 558, 567, 852
MalI GATC 3 cut(s) 76, 459, 642
MboI GATC 3 cut(s) 74, 457, 640
MboII GAAGA 5 cut(s) 230, 256, 637, 850, 892
MflI RGATCY 1 cut(s) 640
MhlI GDGCHC 2 cut(s) 672, 802
MluCI AATT 7 cut(s) 94, 200, 353, 465, 579, 718, 840
MlyI GAGTC 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 353
MseI TTAA 1 cut(s) 806
MspA1I CMGCKG 3 cut(s) 26, 116, 184
MspR9I CCNGG 2 cut(s) 775, 790
MvaI CCWGG 2 cut(s) 775, 790
MwoI GCNNNNNNNGC 4 cut(s) 29, 110, 193, 900
NcoI CCATGG 1 cut(s) 898
NdeII GATC 3 cut(s) 74, 457, 640
NlaIII CATG 4 cut(s) 289, 704, 734, 902
NlaIV GGNNCC 4 cut(s) 112, 444, 771, 904
NmuCI GTSAC 2 cut(s) 567, 852
NspI RCATGY 1 cut(s) 289
NspV TTCGAA 1 cut(s) 861
PdmI GAANNNNTTC 1 cut(s) 353
PfeI GAWTC 2 cut(s) 481, 503
PkrI GCNGC 7 cut(s) 25, 103, 115, 118, 186, 695, 817
PleI GAGTC 1 cut(s) 574
PpsI GAGTC 1 cut(s) 574
PpuMI RGGWCCY 2 cut(s) 443, 769
Psp5II RGGWCCY 2 cut(s) 443, 769
Psp6I CCWGG 2 cut(s) 773, 788
PspGI CCWGG 2 cut(s) 773, 788
PspN4I GGNNCC 4 cut(s) 112, 444, 771, 904
PspPI GGNCC 2 cut(s) 443, 769
PspPPI RGGWCCY 2 cut(s) 443, 769
PsuI RGATCY 1 cut(s) 640
PvuII CAGCTG 2 cut(s) 26, 184
RsaI GTAC 2 cut(s) 421, 548
RsaNI GTAC 2 cut(s) 420, 547
SalI GTCGAC 1 cut(s) 617
SaqAI TTAA 1 cut(s) 806
SatI GCNGC 7 cut(s) 24, 102, 114, 117, 185, 694, 816
Sau3AI GATC 3 cut(s) 74, 457, 640
Sau96I GGNCC 2 cut(s) 443, 769
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 775, 790
SduI GDGCHC 2 cut(s) 672, 802
SfaNI GCATC 3 cut(s) 523, 611, 903
SfuI TTCGAA 1 cut(s) 861
SinI GGWCC 2 cut(s) 443, 769
SmlI CTYRAG 1 cut(s) 318
SmoI CTYRAG 1 cut(s) 318
Sse9I AATT 7 cut(s) 94, 200, 353, 465, 579, 718, 840
SsiI CCGC 8 cut(s) 102, 114, 196, 414, 449, 510, 696, 816
SspMI CTAG 2 cut(s) 638, 834
StyD4I CCNGG 2 cut(s) 773, 788
StyI CCWWGG 1 cut(s) 898
TaiI ACGT 1 cut(s) 225
TaqI TCGA 3 cut(s) 364, 618, 861
TasI AATT 7 cut(s) 94, 200, 353, 465, 579, 718, 840
TatI WGTACW 1 cut(s) 546
TauI GCSGC 3 cut(s) 104, 116, 818
TfiI GAWTC 2 cut(s) 481, 503
Tru1I TTAA 1 cut(s) 806
Tru9I TTAA 1 cut(s) 806
TscAI CASTG 3 cut(s) 111, 166, 743
TseFI GTSAC 2 cut(s) 567, 852
TseI GCWGC 4 cut(s) 23, 116, 184, 693
Tsp45I GTSAC 2 cut(s) 567, 852
TspDTI ATGAA 1 cut(s) 26
TspRI CASTG 3 cut(s) 111, 166, 743
VpaK11BI GGWCC 2 cut(s) 443, 769
XapI RAATTY 2 cut(s) 200, 840
XceI RCATGY 1 cut(s) 289
XcmI CCANNNNNNNNNTGG 1 cut(s) 737
XmiI GTMKAC 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 353
XspI CTAG 2 cut(s) 638, 834
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.