Rh5BG469400

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0018617
Physical Location & Seq
Reverse (-)
74767534 .. 74770174
2641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG469400.1

Sequence Viewer

Length: 1113 bp
ATGGGGCTTTTTCACATGTTGCTGCTTGCATCATTGATCATGATCCAGGTGGGGAACATCGATTCCAAGTGCTTTTCTGCAATATTTAATTTTGGTGACTCCACTTCGGATACGGGTGAAGACCATGCAGCCTTTCCAGACCACCTGCCGGTACCTTATGGGTCTACATTCTTCAAGAAACCTGCCGGAAGAGCTTCAGATGGCCGACTGATCATCGACTTTCTGGCTGAAGCTTTGGGACTTCACTTTTTGACACCTTATCTGAAGCCAGAGGGATCATTCAAAAACGGCGTCAATTTTGCCGCAGTGGGAGCCGCTGCGCTTCCTCCAAACAGCTCCTTTCCTACTTTGTTTCCTCTCAGTGAGTTCAATCTGGGTGTCCAGCTACAACAAATGCGGGAATTCAAGTCCATTGTGGAAGAAGTCCATAAGCACGGGTCTAAGAAGAGGTTTCCTTCTCAGAAGGTCTTTGGAAAGTCTTTATACATGCTCTATATAGGCCAAGCCGATATAGTCTCTCAAGTGGAAACGCTTGGTGCTGATGGAGTGAAGCAATTCTTTCCCCAAATGGTCTCCTCTATTTCTCACACTATACGGGAGCTGTATGGCTTGGGCGGGCGTACATTTTGGGTGTTCGGTGTGGGACCTGCGGGATGTGATCCTATAATTAGGGTTATAGTGAATCAAGCCGGTTCTTATCTTGATTCAGCTGGATGTTATTTTCCTTTGAATGATGCAGTTTCGGAGTACAACAAGCTGTTACGGGAGTCACTCTGGGAAATTAGAGGCGACAACCCAGAAGCATCCGTCATATACGTCGACACCTTCAGCGTGAAGCTAGATCTCTTTAAGAACCCGACACACTATGGGCTCAAATATGGAGCAAAAGTTTGCTGCGGACATGGAGGAGGTCTTTACAATTTTGACCTCCATGCTCCGTGTGGGTGTCCAAATGCAACGGCTTGCAAGGACCCATGGAACTATGTTTCCTGGGATGGAACACATTTAACCGAAGCCGCAAACAGAGTGATTGCTAGGGAAATTCTGAATGGGTCACTCTTCGAACCCCCGTTTCCCTTGAAGAAGCTCTGTCGCATCCATGGTTCCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

370

Amino Acids

40.58

Weight (kDa)

6.63

Isoelectric Point (pI)

28.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 348 5.4e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 153
Acc36I ACCTGC 3 cut(s) 153, 190, 655
Acc65I GGTACC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 151
AccI GTMKAC 2 cut(s) 164, 819
AciI CCGC 7 cut(s) 303, 315, 397, 615, 650, 897, 1017
AclWI GGATC 3 cut(s) 37, 283, 653
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 2 cut(s) 401, 1041
AcuI CTGAAG 4 cut(s) 180, 249, 284, 811
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 3 cut(s) 153, 622, 749
AfiI CCNNNNNNNGG 1 cut(s) 148
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 6 cut(s) 175, 283, 370, 406, 730, 1081
AjnI CCWGG 2 cut(s) 45, 989
AloI GAACNNNNNNTCC 4 cut(s) 47, 79, 971, 1003
AluBI AGCT 9 cut(s) 194, 233, 336, 385, 601, 710, 757, 838, 1087
AluI AGCT 9 cut(s) 194, 233, 336, 385, 601, 710, 757, 838, 1087
Alw26I GTCTC 2 cut(s) 520, 577
AlwI GGATC 3 cut(s) 37, 283, 653
AoxI GGCC 2 cut(s) 202, 499
ApeKI GCWGC 4 cut(s) 22, 128, 317, 894
ApoI RAATTY 2 cut(s) 401, 1041
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 2 cut(s) 193, 554
Asp718I GGTACC 1 cut(s) 151
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 2 cut(s) 644, 970
AsuHPI GGTGA 2 cut(s) 107, 128
AsuII TTCGAA 1 cut(s) 1062
AvaII GGWCC 2 cut(s) 644, 970
BanI GGYRCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 873
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 4 cut(s) 9, 140, 304, 881
BccI CCATC 3 cut(s) 194, 536, 989
BceAI ACGGC 2 cut(s) 304, 975
BciT130I CCWGG 2 cut(s) 47, 991
BciVI GTATCC 1 cut(s) 103
BclI TGATCA 2 cut(s) 36, 210
BcoDI GTCTC 2 cut(s) 520, 577
BfaI CTAG 2 cut(s) 839, 1035
BfuAI ACCTGC 3 cut(s) 153, 190, 655
BfuI GTATCC 1 cut(s) 103
BglII AGATCT 1 cut(s) 841
BisI GCNGC 7 cut(s) 23, 129, 303, 315, 318, 895, 1017
BlsI GCNGC 7 cut(s) 24, 130, 304, 316, 319, 896, 1018
Bme1390I CCNGG 2 cut(s) 47, 991
Bme18I GGWCC 2 cut(s) 644, 970
BmgT120I GGNCC 2 cut(s) 644, 970
BmiI GGNNCC 5 cut(s) 153, 313, 645, 972, 1105
BmrFI CCNGG 2 cut(s) 47, 991
BmsI GCATC 4 cut(s) 38, 724, 812, 1104
BpiI GAAGAC 1 cut(s) 126
Bpu14I TTCGAA 1 cut(s) 1062
BpuEI CTTGAG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 577
BsaJI CCNNGG 3 cut(s) 974, 990, 1099
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse118I RCCGGY 2 cut(s) 148, 689
Bse8I GATNNNNATC 1 cut(s) 41
BseBI CCWGG 2 cut(s) 47, 991
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 3 cut(s) 974, 990, 1099
BseGI GGATG 5 cut(s) 659, 719, 803, 1000, 1095
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 373, 473
BseRI GAGGAG 2 cut(s) 565, 921
BseXI GCAGC 4 cut(s) 9, 140, 304, 881
BshFI GGCC 2 cut(s) 204, 501
BshNI GGYRCC 1 cut(s) 151
BshVI ATCGAT 1 cut(s) 60
BsiSI CCGG 3 cut(s) 149, 186, 690
BslFI GGGAC 2 cut(s) 252, 657
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 2 cut(s) 520, 577
BsmFI GGGAC 2 cut(s) 252, 657
BsnI GGCC 2 cut(s) 204, 501
Bso31I GGTCTC 1 cut(s) 577
Bsp119I TTCGAA 1 cut(s) 1062
Bsp1286I GDGCHC 1 cut(s) 873
Bsp143I GATC 6 cut(s) 36, 42, 210, 275, 658, 841
Bsp19I CCATGG 2 cut(s) 974, 1099
BspACI CCGC 7 cut(s) 303, 315, 397, 615, 650, 897, 1017
BspANI GGCC 2 cut(s) 204, 501
BspCNI CTCAG 2 cut(s) 372, 472
BspDI ATCGAT 1 cut(s) 60
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 5 cut(s) 153, 313, 645, 972, 1105
BspMI ACCTGC 3 cut(s) 153, 190, 655
BspPI GGATC 3 cut(s) 37, 283, 653
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 1062
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 577
BsrFI RCCGGY 2 cut(s) 148, 689
BssAI RCCGGY 2 cut(s) 148, 689
BssECI CCNNGG 3 cut(s) 974, 990, 1099
BssMI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 2 cut(s) 974, 1099
Bst2UI CCWGG 2 cut(s) 47, 991
Bst6I CTCTTC 3 cut(s) 184, 440, 1064
BstACI GRCGYC 1 cut(s) 291
BstBI TTCGAA 1 cut(s) 1062
BstC8I GCNNGC 3 cut(s) 27, 617, 964
BstDEI CTNAG 3 cut(s) 359, 441, 459
BstDSI CCRYGG 2 cut(s) 974, 1099
BstF5I GGATG 5 cut(s) 659, 719, 803, 1000, 1095
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 6 cut(s) 39, 45, 213, 278, 661, 844
BstMAI GTCTC 2 cut(s) 520, 577
BstMBI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
BstMWI GCNNNNNNNGC 2 cut(s) 191, 311
BstNI CCWGG 2 cut(s) 47, 991
BstNSI RCATGY 2 cut(s) 19, 490
BstSCI CCNGG 2 cut(s) 45, 989
BstV1I GCAGC 4 cut(s) 9, 140, 304, 881
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 841
BstYI RGATCY 1 cut(s) 841
Bsu15I ATCGAT 1 cut(s) 60
BsuI GTATCC 1 cut(s) 103
BsuRI GGCC 2 cut(s) 204, 501
BsuTUI ATCGAT 1 cut(s) 60
BtgI CCRYGG 2 cut(s) 974, 1099
BtsCI GGATG 5 cut(s) 659, 719, 803, 1000, 1095
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 2 cut(s) 312, 367
BveI ACCTGC 3 cut(s) 153, 190, 655
Cac8I GCNNGC 3 cut(s) 27, 617, 964
CciI TCATGA 1 cut(s) 39
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 2 cut(s) 148, 689
Cfr13I GGNCC 2 cut(s) 644, 970
ClaI ATCGAT 1 cut(s) 60
CseI GACGC 1 cut(s) 280
Csp6I GTAC 3 cut(s) 152, 621, 748
CviAII CATG 8 cut(s) 16, 40, 125, 487, 902, 932, 975, 1100
CviQI GTAC 3 cut(s) 152, 621, 748
DdeI CTNAG 3 cut(s) 359, 441, 459
DpnI GATC 6 cut(s) 38, 44, 212, 277, 660, 843
DpnII GATC 6 cut(s) 36, 42, 210, 275, 658, 841
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 3 cut(s) 184, 440, 1064
EarI CTCTTC 3 cut(s) 184, 440, 1064
Eco130I CCWWGG 2 cut(s) 974, 1099
Eco24I GRGCYC 1 cut(s) 873
Eco31I GGTCTC 1 cut(s) 577
Eco47I GGWCC 2 cut(s) 644, 970
Eco57I CTGAAG 4 cut(s) 180, 249, 284, 811
EcoO109I RGGNCCY 2 cut(s) 644, 970
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 2 cut(s) 45, 989
EcoT14I CCWWGG 2 cut(s) 974, 1099
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 2 cut(s) 974, 1099
FaeI CATG 8 cut(s) 19, 43, 128, 490, 905, 935, 978, 1103
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 2 cut(s) 252, 657
FatI CATG 8 cut(s) 15, 39, 124, 486, 901, 931, 974, 1099
FauI CCCGC 3 cut(s) 390, 608, 643
FbaI TGATCA 2 cut(s) 36, 210
FblI GTMKAC 2 cut(s) 164, 819
Fnu4HI GCNGC 7 cut(s) 23, 129, 303, 315, 318, 895, 1017
FokI GGATG 5 cut(s) 666, 726, 790, 1007, 1082
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 7 cut(s) 23, 129, 303, 315, 318, 895, 1017
FspBI CTAG 2 cut(s) 839, 1035
GlaI GCGC 1 cut(s) 321
GluI GCNGC 7 cut(s) 23, 129, 303, 315, 318, 895, 1017
HaeIII GGCC 2 cut(s) 204, 501
HapII CCGG 3 cut(s) 149, 186, 690
HgaI GACGC 1 cut(s) 280
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 8 cut(s) 19, 43, 128, 490, 905, 935, 978, 1103
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 5 cut(s) 62, 98, 682, 704, 767
HpaII CCGG 3 cut(s) 149, 186, 690
HphI GGTGA 2 cut(s) 107, 128
Hpy166II GTNNAC 2 cut(s) 165, 820
Hpy188I TCNGA 6 cut(s) 109, 199, 264, 462, 745, 1047
Hpy188III TCNNGA 4 cut(s) 40, 137, 175, 701
Hpy8I GTNNAC 2 cut(s) 165, 820
Hpy99I CGWCG 1 cut(s) 821
HpyAV CCTTC 3 cut(s) 457, 465, 835
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 6 cut(s) 29, 80, 128, 737, 956, 966
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 311
HpyF3I CTNAG 3 cut(s) 359, 441, 459
HpySE526I ACGT 1 cut(s) 816
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 8 cut(s) 19, 43, 128, 490, 905, 935, 978, 1103
HspAI GCGC 1 cut(s) 320
KpnI GGTACC 1 cut(s) 155
Ksp22I TGATCA 2 cut(s) 36, 210
Kzo9I GATC 6 cut(s) 36, 42, 210, 275, 658, 841
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 5 cut(s) 311, 341, 598, 881, 940
Lsp1109I GCAGC 4 cut(s) 9, 140, 304, 881
LweI GCATC 4 cut(s) 38, 724, 812, 1104
MaeI CTAG 2 cut(s) 839, 1035
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 4 cut(s) 95, 759, 768, 1053
MalI GATC 6 cut(s) 38, 44, 212, 277, 660, 843
MboI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
MboII GAAGA 7 cut(s) 131, 163, 201, 431, 457, 1051, 1093
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 1 cut(s) 873
MluCI AATT 8 cut(s) 88, 295, 401, 554, 666, 780, 919, 1041
MlyI GAGTC 2 cut(s) 92, 776
MnlI CCTC 9 cut(s) 265, 336, 366, 441, 586, 779, 899, 902, 938
MroXI GAANNNNTTC 2 cut(s) 193, 554
MseI TTAA 3 cut(s) 87, 849, 1007
MspA1I CMGCKG 2 cut(s) 317, 710
MspI CCGG 3 cut(s) 149, 186, 690
MspR9I CCNGG 2 cut(s) 47, 991
MvaI CCWGG 2 cut(s) 47, 991
MwoI GCNNNNNNNGC 2 cut(s) 191, 311
NcoI CCATGG 2 cut(s) 974, 1099
NdeII GATC 6 cut(s) 36, 42, 210, 275, 658, 841
NlaIII CATG 8 cut(s) 19, 43, 128, 490, 905, 935, 978, 1103
NlaIV GGNNCC 5 cut(s) 153, 313, 645, 972, 1105
NmuCI GTSAC 3 cut(s) 95, 768, 1053
NspI RCATGY 2 cut(s) 19, 490
NspV TTCGAA 1 cut(s) 1062
PagI TCATGA 1 cut(s) 39
PaqCI CACCTGC 1 cut(s) 153
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 193, 554
PfeI GAWTC 3 cut(s) 62, 682, 704
PkrI GCNGC 7 cut(s) 24, 130, 304, 316, 319, 896, 1018
PleI GAGTC 2 cut(s) 92, 775
PpsI GAGTC 2 cut(s) 92, 775
PpuMI RGGWCCY 2 cut(s) 644, 970
PscI ACATGT 1 cut(s) 15
Psp5II RGGWCCY 2 cut(s) 644, 970
Psp6I CCWGG 2 cut(s) 45, 989
PspGI CCWGG 2 cut(s) 45, 989
PspN4I GGNNCC 5 cut(s) 153, 313, 645, 972, 1105
PspPI GGNCC 2 cut(s) 644, 970
PspPPI RGGWCCY 2 cut(s) 644, 970
PsuI RGATCY 1 cut(s) 841
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 3 cut(s) 153, 622, 749
RsaNI GTAC 3 cut(s) 152, 621, 748
SalI GTCGAC 1 cut(s) 818
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 87, 849, 1007
SatI GCNGC 7 cut(s) 23, 129, 303, 315, 318, 895, 1017
Sau3AI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
Sau96I GGNCC 2 cut(s) 644, 970
SchI GAGTC 2 cut(s) 92, 776
ScrFI CCNGG 2 cut(s) 47, 991
SduI GDGCHC 1 cut(s) 873
SfaNI GCATC 4 cut(s) 38, 724, 812, 1104
SfuI TTCGAA 1 cut(s) 1062
SinI GGWCC 2 cut(s) 644, 970
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
Sse9I AATT 8 cut(s) 88, 295, 401, 554, 666, 780, 919, 1041
SsiI CCGC 7 cut(s) 303, 315, 397, 615, 650, 897, 1017
SspI AATATT 1 cut(s) 84
SspMI CTAG 2 cut(s) 839, 1035
StyD4I CCNGG 2 cut(s) 45, 989
StyI CCWWGG 2 cut(s) 974, 1099
TaiI ACGT 1 cut(s) 819
TaqI TCGA 4 cut(s) 60, 216, 819, 1062
TasI AATT 8 cut(s) 88, 295, 401, 554, 666, 780, 919, 1041
TatI WGTACW 1 cut(s) 747
TauI GCSGC 3 cut(s) 305, 317, 1019
TfiI GAWTC 3 cut(s) 62, 682, 704
Tru1I TTAA 3 cut(s) 87, 849, 1007
Tru9I TTAA 3 cut(s) 87, 849, 1007
TscAI CASTG 2 cut(s) 312, 367
TseFI GTSAC 3 cut(s) 95, 768, 1053
TseI GCWGC 4 cut(s) 22, 128, 317, 894
Tsp45I GTSAC 3 cut(s) 95, 768, 1053
TspGWI ACGGA 2 cut(s) 796, 927
TspRI CASTG 2 cut(s) 312, 367
VpaK11BI GGWCC 2 cut(s) 644, 970
XapI RAATTY 2 cut(s) 401, 1041
XceI RCATGY 2 cut(s) 19, 490
XcmI CCANNNNNNNNNTGG 1 cut(s) 938
XmiI GTMKAC 2 cut(s) 164, 819
XmnI GAANNNNTTC 2 cut(s) 193, 554
XspI CTAG 2 cut(s) 839, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.