Rroxscaffold_1G00011960

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0018617
Physical Location & Seq
Forward (+)
14914623 .. 14916873
2251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00011960.1

Sequence Viewer

Length: 1080 bp
ATGGGGCTTTTTCACATGTTGCTGCTTGTATCATTGATCATGATCCAGGTGGGGAACATCGATTCCAAGTGCTTTTCTGCAATATTTAATTTTGGTGACTCCACTTCGGATACGGGTGAAGACCATGCAGCCTTTCCGGACCACCTGCCGGTACCTTATGGGTCTACATTCTTCAAGAAACCTGCCGGAAGAGCTTCAGATGGCCGACTGATCATCGACTTTCTGGCTGAAGCTTTGGGACTTCACTTTTTGACCCCTTATCTGAAGCCAGAGGGATCATTCAAAAACGGCGTCAATTTTGCCGCAGTGGGAGCCGCTGCGCTTCCTCCAAACAGCTCCTTTCCTACTTTGTTTCCTCTCAGTGTGTTCAATCTGGGTGTCCAGCTGCAACAAATGCTGGAATTCAAGTCCATTGTGGAAGAAGTCCATAAGCACGGGTCTAAGAAGAGGTTTCCTTCTCCGAAGGTCTTTGGAGAGTCTTTATACATGCTCTATATAGGCCAAGCCGATATAGTCTCTCAAGTGGAAACGCTTGGTGCTGATGGAGTGAAGCAATTCTTTCCTCGAATGGTCTCCTCTATTTCTCACACTATACGGGAGCTGTATGGCTTGGGCGGGCGTACATTTTGGGTGTTCGGTGTGGGACCTGCGGGATGTGATCCTATAATTAGGGTTATAGTGAATCAGGCCAGTTCTTATCTTGATTCAGGCGGATGTTATTTTCCTTTGAATGATGCTGTTTCGGAGTACAACAAGTTGTTACGGGAGTCACTCTGGGAAATTAGAGGCGAAAACCCAGAAGCATCCATCATATATGTCGACACCTTCAGCGTGAAGCTAGATCTCTTCAAGAACCCGACACACTATGGGCTCAAATATGGAGCAAAAGTTTGCTGCGGACATGGAGGAGGTCTTTACAATTTTGACCTCCATGCTCAGTGTGGGTGTCCAAATGCAACGGCTTGCAAGGACCCCTGGAGCTATGTTTCCTGGGATGGAGCACATTTAACCGAAGCCGCAAACAGATTGATTGCTAGGGAAATTCTGAATGGGTCACTCTTCGAACCCCGTTTCCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

359

Amino Acids

39.38

Weight (kDa)

6.12

Isoelectric Point (pI)

28.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 28 - 348 1.3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 153
Acc36I ACCTGC 3 cut(s) 153, 190, 655
Acc65I GGTACC 1 cut(s) 151
AccB1I GGYRCC 1 cut(s) 151
AccI GTMKAC 2 cut(s) 164, 819
AccIII TCCGGA 1 cut(s) 136
AciI CCGC 7 cut(s) 303, 315, 615, 650, 711, 897, 1017
AclWI GGATC 3 cut(s) 37, 283, 653
AcoI YGGCCR 1 cut(s) 202
AcsI RAATTY 2 cut(s) 401, 1041
AcuI CTGAAG 4 cut(s) 180, 249, 284, 811
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 3 cut(s) 153, 622, 749
AfiI CCNNNNNNNGG 1 cut(s) 148
AflIII ACRYGT 1 cut(s) 15
AgsI TTSAA 6 cut(s) 175, 283, 370, 406, 730, 850
AjnI CCWGG 3 cut(s) 45, 974, 989
AloI GAACNNNNNNTCC 3 cut(s) 47, 79, 1056
AluBI AGCT 7 cut(s) 194, 233, 336, 385, 601, 838, 981
AluI AGCT 7 cut(s) 194, 233, 336, 385, 601, 838, 981
Alw21I GWGCWC 1 cut(s) 1003
Alw26I GTCTC 2 cut(s) 520, 577
AlwI GGATC 3 cut(s) 37, 283, 653
Aor13HI TCCGGA 1 cut(s) 136
AoxI GGCC 3 cut(s) 202, 499, 687
ApeKI GCWGC 5 cut(s) 22, 128, 317, 385, 894
ApoI RAATTY 2 cut(s) 401, 1041
ArsI GACNNNNNNTTYG 2 cut(s) 276, 308
Asp700I GAANNNNTTC 2 cut(s) 193, 554
Asp718I GGTACC 1 cut(s) 151
AspLEI GCGC 1 cut(s) 322
AspS9I GGNCC 3 cut(s) 139, 644, 970
AsuHPI GGTGA 2 cut(s) 107, 128
AsuII TTCGAA 1 cut(s) 1062
AvaII GGWCC 3 cut(s) 139, 644, 970
BanI GGYRCC 1 cut(s) 151
BanII GRGCYC 1 cut(s) 873
BbsI GAAGAC 1 cut(s) 126
Bbv12I GWGCWC 1 cut(s) 1003
BbvI GCAGC 5 cut(s) 9, 140, 304, 372, 881
BccI CCATC 4 cut(s) 194, 536, 815, 989
BceAI ACGGC 2 cut(s) 304, 975
BciT130I CCWGG 3 cut(s) 47, 976, 991
BciVI GTATCC 1 cut(s) 103
BclI TGATCA 2 cut(s) 36, 210
BcoDI GTCTC 2 cut(s) 520, 577
BfaI CTAG 2 cut(s) 839, 1035
BfuAI ACCTGC 3 cut(s) 153, 190, 655
BfuI GTATCC 1 cut(s) 103
BglII AGATCT 1 cut(s) 841
BisI GCNGC 8 cut(s) 23, 129, 303, 315, 318, 386, 895, 1017
BlsI GCNGC 8 cut(s) 24, 130, 304, 316, 319, 387, 896, 1018
Bme1390I CCNGG 3 cut(s) 47, 976, 991
Bme18I GGWCC 3 cut(s) 139, 644, 970
BmgT120I GGNCC 3 cut(s) 139, 644, 970
BmiI GGNNCC 4 cut(s) 153, 313, 645, 972
BmrFI CCNGG 3 cut(s) 47, 976, 991
BmsI GCATC 2 cut(s) 724, 812
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 997
Bpu14I TTCGAA 1 cut(s) 1062
BpuEI CTTGAG 1 cut(s) 504
Bsa29I ATCGAT 1 cut(s) 60
BsaBI GATNNNNATC 1 cut(s) 41
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 1 cut(s) 577
BsaJI CCNNGG 2 cut(s) 974, 990
BsaWI WCCGGW 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 873, 903
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 1 cut(s) 690
Bse8I GATNNNNATC 1 cut(s) 41
BseAI TCCGGA 1 cut(s) 136
BseBI CCWGG 3 cut(s) 47, 976, 991
BseCI ATCGAT 1 cut(s) 60
BseDI CCNNGG 2 cut(s) 974, 990
BseGI GGATG 4 cut(s) 659, 719, 803, 1000
BseJI GATNNNNATC 1 cut(s) 41
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 2 cut(s) 373, 950
BseNI ACTGG 1 cut(s) 690
BseRI GAGGAG 2 cut(s) 565, 921
BseXI GCAGC 5 cut(s) 9, 140, 304, 372, 881
BshFI GGCC 3 cut(s) 204, 501, 689
BshNI GGYRCC 1 cut(s) 151
BshVI ATCGAT 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 1003
BsiSI CCGG 3 cut(s) 137, 149, 186
BslFI GGGAC 2 cut(s) 252, 657
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 2 cut(s) 520, 577
BsmFI GGGAC 2 cut(s) 252, 657
BsnI GGCC 3 cut(s) 204, 501, 689
Bso31I GGTCTC 1 cut(s) 577
Bsp119I TTCGAA 1 cut(s) 1062
Bsp1286I GDGCHC 2 cut(s) 873, 1003
Bsp13I TCCGGA 1 cut(s) 136
Bsp143I GATC 6 cut(s) 36, 42, 210, 275, 658, 841
BspACI CCGC 7 cut(s) 303, 315, 615, 650, 711, 897, 1017
BspANI GGCC 3 cut(s) 204, 501, 689
BspCNI CTCAG 2 cut(s) 372, 949
BspDI ATCGAT 1 cut(s) 60
BspEI TCCGGA 1 cut(s) 136
BspHI TCATGA 1 cut(s) 39
BspLI GGNNCC 4 cut(s) 153, 313, 645, 972
BspMI ACCTGC 3 cut(s) 153, 190, 655
BspPI GGATC 3 cut(s) 37, 283, 653
BspQI GCTCTTC 1 cut(s) 184
BspT104I TTCGAA 1 cut(s) 1062
BspT107I GGYRCC 1 cut(s) 151
BspTNI GGTCTC 1 cut(s) 577
BsrFI RCCGGY 1 cut(s) 148
BsrI ACTGG 1 cut(s) 690
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 2 cut(s) 974, 990
BssMI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
BssNI GRCGYC 1 cut(s) 291
Bst2UI CCWGG 3 cut(s) 47, 976, 991
Bst6I CTCTTC 4 cut(s) 184, 440, 851, 1064
BstACI GRCGYC 1 cut(s) 291
BstAPI GCANNNNNTGC 1 cut(s) 394
BstBI TTCGAA 1 cut(s) 1062
BstC8I GCNNGC 2 cut(s) 617, 964
BstDEI CTNAG 3 cut(s) 359, 441, 936
BstF5I GGATG 4 cut(s) 659, 719, 803, 1000
BstHHI GCGC 1 cut(s) 322
BstKTI GATC 6 cut(s) 39, 45, 213, 278, 661, 844
BstMAI GTCTC 2 cut(s) 520, 577
BstMBI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
BstMWI GCNNNNNNNGC 3 cut(s) 191, 311, 394
BstNI CCWGG 3 cut(s) 47, 976, 991
BstNSI RCATGY 2 cut(s) 19, 490
BstSCI CCNGG 3 cut(s) 45, 974, 989
BstV1I GCAGC 5 cut(s) 9, 140, 304, 372, 881
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 841
BstYI RGATCY 1 cut(s) 841
Bsu15I ATCGAT 1 cut(s) 60
BsuI GTATCC 1 cut(s) 103
BsuRI GGCC 3 cut(s) 204, 501, 689
BsuTUI ATCGAT 1 cut(s) 60
BtsCI GGATG 4 cut(s) 659, 719, 803, 1000
BtsI GCAGTG 1 cut(s) 312
BtsIMutI CAGTG 3 cut(s) 312, 367, 944
BveI ACCTGC 3 cut(s) 153, 190, 655
Cac8I GCNNGC 2 cut(s) 617, 964
CciI TCATGA 1 cut(s) 39
CfoI GCGC 1 cut(s) 322
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 3 cut(s) 139, 644, 970
ClaI ATCGAT 1 cut(s) 60
CseI GACGC 1 cut(s) 280
Csp6I GTAC 3 cut(s) 152, 621, 748
CviAII CATG 6 cut(s) 16, 40, 125, 487, 902, 932
CviQI GTAC 3 cut(s) 152, 621, 748
DdeI CTNAG 3 cut(s) 359, 441, 936
DpnI GATC 6 cut(s) 38, 44, 212, 277, 660, 843
DpnII GATC 6 cut(s) 36, 42, 210, 275, 658, 841
EaeI YGGCCR 1 cut(s) 202
Eam1104I CTCTTC 4 cut(s) 184, 440, 851, 1064
EarI CTCTTC 4 cut(s) 184, 440, 851, 1064
EciI GGCGGA 1 cut(s) 726
Eco24I GRGCYC 1 cut(s) 873
Eco31I GGTCTC 1 cut(s) 577
Eco47I GGWCC 3 cut(s) 139, 644, 970
Eco57I CTGAAG 4 cut(s) 180, 249, 284, 811
EcoO109I RGGNCCY 2 cut(s) 644, 970
EcoRI GAATTC 1 cut(s) 401
EcoRII CCWGG 3 cut(s) 45, 974, 989
EcoT38I GRGCYC 1 cut(s) 873
FaeI CATG 6 cut(s) 19, 43, 128, 490, 905, 935
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 2 cut(s) 252, 657
FatI CATG 6 cut(s) 15, 39, 124, 486, 901, 931
FauI CCCGC 2 cut(s) 608, 643
FbaI TGATCA 2 cut(s) 36, 210
FblI GTMKAC 2 cut(s) 164, 819
Fnu4HI GCNGC 8 cut(s) 23, 129, 303, 315, 318, 386, 895, 1017
FokI GGATG 4 cut(s) 666, 726, 790, 1007
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 8 cut(s) 23, 129, 303, 315, 318, 386, 895, 1017
FspBI CTAG 2 cut(s) 839, 1035
GlaI GCGC 1 cut(s) 321
GluI GCNGC 8 cut(s) 23, 129, 303, 315, 318, 386, 895, 1017
GsuI CTGGAG 1 cut(s) 997
HaeIII GGCC 3 cut(s) 204, 501, 689
HapII CCGG 3 cut(s) 137, 149, 186
HgaI GACGC 1 cut(s) 280
HhaI GCGC 1 cut(s) 322
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 6 cut(s) 19, 43, 128, 490, 905, 935
Hin6I GCGC 1 cut(s) 320
HinP1I GCGC 1 cut(s) 320
HincII GTYRAC 1 cut(s) 820
HindII GTYRAC 1 cut(s) 820
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 6 cut(s) 62, 98, 476, 682, 704, 767
HpaII CCGG 3 cut(s) 137, 149, 186
HphI GGTGA 2 cut(s) 107, 128
Hpy166II GTNNAC 2 cut(s) 165, 820
Hpy188I TCNGA 6 cut(s) 109, 199, 264, 462, 745, 1047
Hpy188III TCNNGA 5 cut(s) 40, 137, 175, 701, 850
Hpy8I GTNNAC 2 cut(s) 165, 820
HpyAV CCTTC 3 cut(s) 457, 465, 835
HpyCH4V TGCA 5 cut(s) 80, 128, 388, 956, 966
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 311, 394
HpyF3I CTNAG 3 cut(s) 359, 441, 936
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 6 cut(s) 19, 43, 128, 490, 905, 935
HspAI GCGC 1 cut(s) 320
Kpn2I TCCGGA 1 cut(s) 136
KpnI GGTACC 1 cut(s) 155
Ksp22I TGATCA 2 cut(s) 36, 210
Kzo9I GATC 6 cut(s) 36, 42, 210, 275, 658, 841
LguI GCTCTTC 1 cut(s) 184
LmnI GCTCC 6 cut(s) 311, 341, 598, 881, 978, 998
Lsp1109I GCAGC 5 cut(s) 9, 140, 304, 372, 881
LweI GCATC 2 cut(s) 724, 812
MaeI CTAG 2 cut(s) 839, 1035
MaeIII GTNAC 4 cut(s) 95, 759, 768, 1053
MalI GATC 6 cut(s) 38, 44, 212, 277, 660, 843
MboI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
MboII GAAGA 7 cut(s) 131, 163, 201, 431, 457, 838, 1051
MflI RGATCY 1 cut(s) 841
MhlI GDGCHC 2 cut(s) 873, 1003
MluCI AATT 8 cut(s) 88, 295, 401, 554, 666, 780, 919, 1041
MlyI GAGTC 3 cut(s) 92, 485, 776
MroI TCCGGA 1 cut(s) 136
MroXI GAANNNNTTC 2 cut(s) 193, 554
MseI TTAA 2 cut(s) 87, 1007
MspA1I CMGCKG 2 cut(s) 317, 385
MspI CCGG 3 cut(s) 137, 149, 186
MspR9I CCNGG 3 cut(s) 47, 976, 991
MvaI CCWGG 3 cut(s) 47, 976, 991
MwoI GCNNNNNNNGC 3 cut(s) 191, 311, 394
NdeII GATC 6 cut(s) 36, 42, 210, 275, 658, 841
NlaIII CATG 6 cut(s) 19, 43, 128, 490, 905, 935
NlaIV GGNNCC 4 cut(s) 153, 313, 645, 972
NmuCI GTSAC 3 cut(s) 95, 768, 1053
NspI RCATGY 2 cut(s) 19, 490
NspV TTCGAA 1 cut(s) 1062
PagI TCATGA 1 cut(s) 39
PaqCI CACCTGC 1 cut(s) 153
PciI ACATGT 1 cut(s) 15
PciSI GCTCTTC 1 cut(s) 184
PdmI GAANNNNTTC 2 cut(s) 193, 554
PfeI GAWTC 3 cut(s) 62, 682, 704
PkrI GCNGC 8 cut(s) 24, 130, 304, 316, 319, 387, 896, 1018
PleI GAGTC 3 cut(s) 92, 484, 775
PpsI GAGTC 3 cut(s) 92, 484, 775
PpuMI RGGWCCY 2 cut(s) 644, 970
PscI ACATGT 1 cut(s) 15
Psp5II RGGWCCY 2 cut(s) 644, 970
Psp6I CCWGG 3 cut(s) 45, 974, 989
PspGI CCWGG 3 cut(s) 45, 974, 989
PspN4I GGNNCC 4 cut(s) 153, 313, 645, 972
PspPI GGNCC 3 cut(s) 139, 644, 970
PspPPI RGGWCCY 2 cut(s) 644, 970
PsuI RGATCY 1 cut(s) 841
PvuII CAGCTG 1 cut(s) 385
RsaI GTAC 3 cut(s) 153, 622, 749
RsaNI GTAC 3 cut(s) 152, 621, 748
SalI GTCGAC 1 cut(s) 818
SapI GCTCTTC 1 cut(s) 184
SaqAI TTAA 2 cut(s) 87, 1007
SatI GCNGC 8 cut(s) 23, 129, 303, 315, 318, 386, 895, 1017
Sau3AI GATC 6 cut(s) 36, 42, 210, 275, 658, 841
Sau96I GGNCC 3 cut(s) 139, 644, 970
SchI GAGTC 3 cut(s) 92, 485, 776
ScrFI CCNGG 3 cut(s) 47, 976, 991
SduI GDGCHC 2 cut(s) 873, 1003
SfaNI GCATC 2 cut(s) 724, 812
SfuI TTCGAA 1 cut(s) 1062
SinI GGWCC 3 cut(s) 139, 644, 970
SmlI CTYRAG 1 cut(s) 519
SmoI CTYRAG 1 cut(s) 519
Sse9I AATT 8 cut(s) 88, 295, 401, 554, 666, 780, 919, 1041
SsiI CCGC 7 cut(s) 303, 315, 615, 650, 711, 897, 1017
SspI AATATT 1 cut(s) 84
SspMI CTAG 2 cut(s) 839, 1035
StyD4I CCNGG 3 cut(s) 45, 974, 989
TaqI TCGA 5 cut(s) 60, 216, 565, 819, 1062
TasI AATT 8 cut(s) 88, 295, 401, 554, 666, 780, 919, 1041
TatI WGTACW 1 cut(s) 747
TauI GCSGC 3 cut(s) 305, 317, 1019
TfiI GAWTC 3 cut(s) 62, 682, 704
Tru1I TTAA 2 cut(s) 87, 1007
Tru9I TTAA 2 cut(s) 87, 1007
TscAI CASTG 3 cut(s) 312, 367, 944
TseFI GTSAC 3 cut(s) 95, 768, 1053
TseI GCWGC 5 cut(s) 22, 128, 317, 385, 894
Tsp45I GTSAC 3 cut(s) 95, 768, 1053
TspRI CASTG 3 cut(s) 312, 367, 944
VpaK11BI GGWCC 3 cut(s) 139, 644, 970
XapI RAATTY 2 cut(s) 401, 1041
XceI RCATGY 2 cut(s) 19, 490
XcmI CCANNNNNNNNNTGG 1 cut(s) 938
XmiI GTMKAC 2 cut(s) 164, 819
XmnI GAANNNNTTC 2 cut(s) 193, 554
XspI CTAG 2 cut(s) 839, 1035
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.