Rorug05G0393900

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0018617
Physical Location & Seq
Reverse (-)
53975912 .. 53995095
19184 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0393900.1

Sequence Viewer

Length: 1407 bp
ATGGAGTTTGGCGAAGAAGGATTACTACAAGAGCCGGTGAGCCCTGGTGGTCAGTTCATCAACAGTTCTGTCTTATCTCTTGCAATTCTTGCCATTTTGGAATCACAGGTTCCAATCGACGATTCTCAAACGTTTTTTCTTCTGAAAAATGTCTTCCTCCCGATCAATCCACGCTTCTCTTCCATCATGGTTGAAGGTGCAGATAAAAAGAATAAGCAGTGGAAGAAGGTTACAGTGAAGGTGGAAGACCACGTCCATGTCCCCATTTTCCCCTCTGGAATGTCACCTGCATCATACGATATTTATTTTGATGAGTATATAACGAAGATAGCATCAGAAACATTCCCACAAAGCAGGCCATTATGGGAACTTCATCTTTTCAAGTACCCAACAAGTCATGCAGCTGGACAAATGATATTCAAGATCCACCATGCCCTTGGCGATGGCTACTCTCTCATGGGCGCTCTTCTCTCTTGCCTCCAGAATGCTCACAATCCTTCCATTCCTCTGACATTTCCGACATTAAAGAGTGCAAAAAATGAAACTAGTAGTCGATCTCGTGCGTTTGAGTTTATGCCGAAGATACTTTCTTCCGTTTTCAACGGTGCATGGGATTTCAGTTGGAGCATTTTGAAGGGCACTTGGGTTGAAGATGATCGAACACCAATAAGGTCCGGTGTTGAAGGAGTTGAGTTTCGACCTGTGTCGGTGTCAACCTTGATGCTGCCTATTGAGGAAATTAAACTTATCAAGAACAAGCTTGGAGTGACGATAAATGATGTGATTACAGGAACAATTTTTATGGGTATCCGAATGTACATTCAAGAGATGAATAGTGAAAAATCAAGTAGCCAAAATTGCACAGCACTGGTGTTGCTGAATACTAGACTTGTTACGGGTTACAAGTCGGTGCAGGAGATGTTGATCGAAACAAATAAGTCTTGGGGAAACCGATTTGTGTTCTTGCAAGTTTCGGTGCCCAAGTCGAGTGAAGTTTCAAAGCCACTGGATTTTGTGTGGGAAGCACATAATATAATCAAGAGACAGAGAAAATCTTCAGCTTGGTATCTCACCACTAGGTTCTGGGACATTTTGAAGAAATTTAGAGGCGCTGAGGCAGCATCTAAATATATCTATAGTACACTGAAAAACACAAGCATAGGAATCACAAATATGATTGGGCCATTGGAACAAATGAGTCTCGCCAATCACCCCATTGAAGGAATTTACTTCATAGTGACTGGTTCACCTCAGTGTCTTTCTGTAACAGCAATGAGTTATATGGGAAAGCTAAGGATTGCTTTGGGAGCTGAAAAAGACTTCATAGATACCAACGTATTGCAAGCATGCATGAAAGATGCATTTCGGGTGATATGTGAAGCTGTGAATGAATTTCCTATCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

468

Amino Acids

52.62

Weight (kDa)

8.4

Isoelectric Point (pI)

46.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WS_DGAT_cat PF03007 96 - 262 1.8e-10 Wax ester synthase/diacylglycerol acyltransferase catalytic domain
WS_DGAT_C PF06974 315 - 458 5.3e-35 WS/DGAT C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018617)

Species Orthologous Gene IDs
rosa_chinensis RchiOBHm_Chr5g0068861
rosa_laevigata RLG00000036014
rosa_multiflora Rmu_sc0006027.1_g000012
rosa_roxburghii Rroxscaffold_1G00011960
rosa_rugosa Rorug05G0393900
rosa_samantha Rh5AG451000 Rh5BG469400 Rh5CG491800 Rh5DG482800
rosa_wichuraiana Rw5G042090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 295
Acc36I ACCTGC 1 cut(s) 295
AccB1I GGYRCC 1 cut(s) 976
AclI AACGTT 1 cut(s) 131
AclWI GGATC 1 cut(s) 418
AcsI RAATTY 3 cut(s) 1100, 1224, 1391
AcuI CTGAAG 1 cut(s) 1041
AfaI GTAC 3 cut(s) 386, 818, 1141
AfiI CCNNNNNNNGG 1 cut(s) 1220
AhlI ACTAGT 1 cut(s) 545
AjiI CACGTC 1 cut(s) 253
AjnI CCWGG 1 cut(s) 43
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AleI CACNNNNGTG 1 cut(s) 1252
AluBI AGCT 6 cut(s) 404, 760, 1061, 1291, 1310, 1382
AluI AGCT 6 cut(s) 404, 760, 1061, 1291, 1310, 1382
Alw26I GTCTC 2 cut(s) 1036, 1205
AlwI GGATC 1 cut(s) 418
AoxI GGCC 2 cut(s) 356, 1181
ApeKI GCWGC 3 cut(s) 401, 724, 1118
ApoI RAATTY 3 cut(s) 1100, 1224, 1391
Asp700I GAANNNNTTC 1 cut(s) 1054
AspLEI GCGC 2 cut(s) 464, 1112
AspS9I GGNCC 2 cut(s) 672, 1181
AsuHPI GGTGA 6 cut(s) 49, 276, 1063, 1202, 1239, 1381
AvaII GGWCC 1 cut(s) 672
BaeGI GKGCMC 2 cut(s) 641, 981
BaeI ACNNNNGTAYC 2 cut(s) 1320, 1353
BanI GGYRCC 1 cut(s) 976
BanII GRGCYC 1 cut(s) 44
BarI GAAGNNNNNNTAC 3 cut(s) 9, 38, 41
BauI CACGAG 1 cut(s) 558
BbsI GAAGAC 2 cut(s) 145, 252
BbvCI CCTCAGC 1 cut(s) 1113
BbvI GCAGC 3 cut(s) 413, 711, 1130
BccI CCATC 2 cut(s) 191, 437
BciT130I CCWGG 1 cut(s) 45
BciVI GTATCC 1 cut(s) 818
BcoDI GTCTC 2 cut(s) 1036, 1205
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 546, 885, 1077
BfmI CTRYAG 1 cut(s) 1135
BfoI RGCGCY 2 cut(s) 465, 1113
BfuAI ACCTGC 1 cut(s) 295
BfuI GTATCC 1 cut(s) 818
BisI GCNGC 3 cut(s) 402, 725, 1119
BlsI GCNGC 3 cut(s) 403, 726, 1120
Bme1390I CCNGG 1 cut(s) 45
Bme18I GGWCC 1 cut(s) 672
BmgBI CACGTC 1 cut(s) 253
BmgT120I GGNCC 2 cut(s) 672, 1181
BmiI GGNNCC 2 cut(s) 111, 978
BmrFI CCNGG 1 cut(s) 45
BmsI GCATC 5 cut(s) 299, 341, 711, 1130, 1348
BoxI GACNNNNGTC 1 cut(s) 703
BpiI GAAGAC 2 cut(s) 145, 252
BpmI CTGGAG 1 cut(s) 464
Bpu10I CCTNAGC 2 cut(s) 1113, 1292
BsaBI GATNNNNATC 1 cut(s) 923
BsaJI CCNNGG 2 cut(s) 43, 436
BsaWI WCCGGW 1 cut(s) 674
Bsc4I CCNNNNNNNGG 1 cut(s) 1220
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 3 cut(s) 873, 1011, 1246
Bse3DI GCAATG 1 cut(s) 1278
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 1 cut(s) 45
BseDI CCNNGG 2 cut(s) 43, 436
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 1 cut(s) 1220
BseMI GCAATG 1 cut(s) 1278
BseMII CTCAG 2 cut(s) 1104, 1265
BseNI ACTGG 3 cut(s) 873, 1011, 1246
BseSI GKGCMC 2 cut(s) 641, 981
BseXI GCAGC 3 cut(s) 413, 711, 1130
BsgI GTGCAG 2 cut(s) 219, 932
BshFI GGCC 2 cut(s) 358, 1183
BshNI GGYRCC 1 cut(s) 976
BsiSI CCGG 2 cut(s) 35, 675
BslFI GGGAC 2 cut(s) 245, 1100
BslI CCNNNNNNNGG 1 cut(s) 1220
BsmAI GTCTC 2 cut(s) 1036, 1205
BsmFI GGGAC 2 cut(s) 245, 1100
BsmI GAATGC 1 cut(s) 490
BsnI GGCC 2 cut(s) 358, 1183
Bsp1286I GDGCHC 3 cut(s) 44, 641, 981
Bsp1407I TGTACA 1 cut(s) 816
Bsp143I GATC 5 cut(s) 162, 423, 554, 655, 924
BspANI GGCC 2 cut(s) 358, 1183
BspCNI CTCAG 2 cut(s) 1105, 1264
BspLI GGNNCC 2 cut(s) 111, 978
BspMI ACCTGC 1 cut(s) 295
BspPI GGATC 1 cut(s) 418
BspQI GCTCTTC 1 cut(s) 471
BspT107I GGYRCC 1 cut(s) 976
BsrDI GCAATG 1 cut(s) 1278
BsrFI RCCGGY 1 cut(s) 34
BsrGI TGTACA 1 cut(s) 816
BsrI ACTGG 3 cut(s) 873, 1011, 1246
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 2 cut(s) 43, 436
BssMI GATC 5 cut(s) 162, 423, 554, 655, 924
BssSI CACGAG 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 558
Bst2UI CCWGG 1 cut(s) 45
Bst4CI ACNGT 3 cut(s) 65, 235, 605
Bst6I CTCTTC 2 cut(s) 184, 471
BstAPI GCANNNNNTGC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 816
BstC8I GCNNGC 3 cut(s) 356, 1344, 1348
BstDEI CTNAG 3 cut(s) 1113, 1251, 1292
BstH2I RGCGCY 2 cut(s) 465, 1113
BstHHI GCGC 2 cut(s) 464, 1112
BstKTI GATC 5 cut(s) 165, 426, 557, 658, 927
BstMAI GTCTC 2 cut(s) 1036, 1205
BstMBI GATC 5 cut(s) 162, 423, 554, 655, 924
BstMWI GCNNNNNNNGC 4 cut(s) 89, 858, 1118, 1307
BstNI CCWGG 1 cut(s) 45
BstNSI RCATGY 1 cut(s) 1350
BstPAI GACNNNNGTC 1 cut(s) 703
BstSCI CCNGG 1 cut(s) 43
BstSFI CTRYAG 1 cut(s) 1135
BstSLI GKGCMC 2 cut(s) 641, 981
BstV1I GCAGC 3 cut(s) 413, 711, 1130
BstV2I GAAGAC 2 cut(s) 145, 252
BstX2I RGATCY 1 cut(s) 423
BstXI CCANNNNNNTGG 1 cut(s) 437
BstYI RGATCY 1 cut(s) 423
BsuI GTATCC 1 cut(s) 818
BsuRI GGCC 2 cut(s) 358, 1183
BtgZI GCGATG 1 cut(s) 456
BtrI CACGTC 1 cut(s) 253
BtsI GCAGTG 1 cut(s) 224
BtsIMutI CAGTG 6 cut(s) 224, 240, 866, 1004, 1142, 1259
BveI ACCTGC 1 cut(s) 295
Cac8I GCNNGC 3 cut(s) 356, 1344, 1348
CfoI GCGC 2 cut(s) 464, 1112
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 672, 1181
Csp6I GTAC 3 cut(s) 385, 817, 1140
CviAII CATG 8 cut(s) 187, 257, 398, 431, 457, 609, 1347, 1351
CviQI GTAC 3 cut(s) 385, 817, 1140
DdeI CTNAG 3 cut(s) 1113, 1251, 1292
DpnI GATC 5 cut(s) 164, 425, 556, 657, 926
DpnII GATC 5 cut(s) 162, 423, 554, 655, 924
Eam1104I CTCTTC 2 cut(s) 184, 471
EarI CTCTTC 2 cut(s) 184, 471
Eco130I CCWWGG 1 cut(s) 436
Eco24I GRGCYC 1 cut(s) 44
Eco47I GGWCC 1 cut(s) 672
Eco57I CTGAAG 1 cut(s) 1041
EcoRII CCWGG 1 cut(s) 43
EcoT14I CCWWGG 1 cut(s) 436
EcoT22I ATGCAT 2 cut(s) 1352, 1363
EcoT38I GRGCYC 1 cut(s) 44
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 1350, 1354
FalI AAGNNNNNCTT 2 cut(s) 1285, 1317
FaqI GGGAC 2 cut(s) 245, 1100
FatI CATG 8 cut(s) 186, 256, 397, 430, 456, 608, 1346, 1350
Fnu4HI GCNGC 3 cut(s) 402, 725, 1119
FriOI GRGCYC 1 cut(s) 44
Fsp4HI GCNGC 3 cut(s) 402, 725, 1119
FspBI CTAG 3 cut(s) 546, 885, 1077
GlaI GCGC 2 cut(s) 463, 1111
GluI GCNGC 3 cut(s) 402, 725, 1119
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 2 cut(s) 465, 1113
HaeIII GGCC 2 cut(s) 358, 1183
HapII CCGG 2 cut(s) 35, 675
HhaI GCGC 2 cut(s) 464, 1112
Hin1II CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 1350, 1354
Hin6I GCGC 2 cut(s) 462, 1110
HinP1I GCGC 2 cut(s) 462, 1110
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HindIII AAGCTT 1 cut(s) 758
HinfI GANTC 4 cut(s) 101, 122, 1164, 1198
HpaII CCGG 2 cut(s) 35, 675
HphI GGTGA 6 cut(s) 49, 276, 1063, 1202, 1239, 1381
Hpy166II GTNNAC 3 cut(s) 714, 1142, 1247
Hpy188I TCNGA 5 cut(s) 144, 337, 510, 519, 812
Hpy188III TCNNGA 7 cut(s) 160, 276, 421, 481, 751, 824, 1039
Hpy8I GTNNAC 3 cut(s) 714, 1142, 1247
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 8 cut(s) 11, 188, 220, 232, 507, 628, 677, 1214
HpyCH4III ACNGT 3 cut(s) 65, 235, 605
HpyCH4IV ACGT 3 cut(s) 131, 252, 1335
HpyF10VI GCNNNNNNNGC 4 cut(s) 89, 858, 1118, 1307
HpyF3I CTNAG 3 cut(s) 1113, 1251, 1292
HpySE526I ACGT 3 cut(s) 131, 252, 1335
Hsp92II CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 1350, 1354
HspAI GCGC 2 cut(s) 462, 1110
Kzo9I GATC 5 cut(s) 162, 423, 554, 655, 924
LguI GCTCTTC 1 cut(s) 471
LmnI GCTCC 2 cut(s) 624, 1307
Lsp1109I GCAGC 3 cut(s) 413, 711, 1130
LweI GCATC 5 cut(s) 299, 341, 711, 1130, 1348
MaeI CTAG 3 cut(s) 546, 885, 1077
MaeII ACGT 3 cut(s) 131, 252, 1335
MaeIII GTNAC 7 cut(s) 229, 282, 766, 892, 899, 1237, 1264
MalI GATC 5 cut(s) 164, 425, 556, 657, 926
MboI GATC 5 cut(s) 162, 423, 554, 655, 924
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 3 cut(s) 44, 641, 981
MluCI AATT 7 cut(s) 84, 738, 795, 856, 1100, 1224, 1391
MlyI GAGTC 1 cut(s) 1207
MmeI TCCRAC 2 cut(s) 542, 602
MnlI CCTC 8 cut(s) 167, 283, 488, 516, 727, 1100, 1108, 1260
Mph1103I ATGCAT 2 cut(s) 1352, 1363
MroXI GAANNNNTTC 1 cut(s) 1054
MseI TTAA 2 cut(s) 524, 741
MslI CAYNNNNRTG 3 cut(s) 255, 1172, 1252
MspA1I CMGCKG 1 cut(s) 404
MspI CCGG 2 cut(s) 35, 675
MspR9I CCNGG 1 cut(s) 45
Mva1269I GAATGC 1 cut(s) 490
MvaI CCWGG 1 cut(s) 45
MwoI GCNNNNNNNGC 4 cut(s) 89, 858, 1118, 1307
NdeII GATC 5 cut(s) 162, 423, 554, 655, 924
NlaIII CATG 8 cut(s) 190, 260, 401, 434, 460, 612, 1350, 1354
NlaIV GGNNCC 2 cut(s) 111, 978
NmuCI GTSAC 3 cut(s) 282, 766, 1237
NsiI ATGCAT 2 cut(s) 1352, 1363
NspI RCATGY 1 cut(s) 1350
OliI CACNNNNGTG 1 cut(s) 1252
PaeI GCATGC 1 cut(s) 1350
PaqCI CACCTGC 1 cut(s) 295
PciSI GCTCTTC 1 cut(s) 471
PctI GAATGC 1 cut(s) 490
PdmI GAANNNNTTC 1 cut(s) 1054
PfeI GAWTC 3 cut(s) 101, 122, 1164
PflFI GACNNNGTC 1 cut(s) 251
PkrI GCNGC 3 cut(s) 403, 726, 1120
PleI GAGTC 1 cut(s) 1206
PpsI GAGTC 1 cut(s) 1206
PshAI GACNNNNGTC 1 cut(s) 703
Psp1406I AACGTT 1 cut(s) 131
Psp6I CCWGG 1 cut(s) 43
PspGI CCWGG 1 cut(s) 43
PspN4I GGNNCC 2 cut(s) 111, 978
PspPI GGNCC 2 cut(s) 672, 1181
PsuI RGATCY 1 cut(s) 423
PsyI GACNNNGTC 1 cut(s) 251
PvuII CAGCTG 1 cut(s) 404
RsaI GTAC 3 cut(s) 386, 818, 1141
RsaNI GTAC 3 cut(s) 385, 817, 1140
RseI CAYNNNNRTG 3 cut(s) 255, 1172, 1252
SapI GCTCTTC 1 cut(s) 471
SaqAI TTAA 2 cut(s) 524, 741
SatI GCNGC 3 cut(s) 402, 725, 1119
Sau3AI GATC 5 cut(s) 162, 423, 554, 655, 924
Sau96I GGNCC 2 cut(s) 672, 1181
SchI GAGTC 1 cut(s) 1207
ScrFI CCNGG 1 cut(s) 45
SduI GDGCHC 3 cut(s) 44, 641, 981
SfaNI GCATC 5 cut(s) 299, 341, 711, 1130, 1348
SfcI CTRYAG 1 cut(s) 1135
SinI GGWCC 1 cut(s) 672
SmiMI CAYNNNNRTG 3 cut(s) 255, 1172, 1252
SpeI ACTAGT 1 cut(s) 545
SphI GCATGC 1 cut(s) 1350
Sse9I AATT 7 cut(s) 84, 738, 795, 856, 1100, 1224, 1391
SspMI CTAG 3 cut(s) 546, 885, 1077
StyD4I CCNGG 1 cut(s) 43
StyI CCWWGG 1 cut(s) 436
TaaI ACNGT 3 cut(s) 65, 235, 605
TaiI ACGT 3 cut(s) 134, 255, 1338
TaqI TCGA 6 cut(s) 117, 553, 658, 697, 927, 986
TasI AATT 7 cut(s) 84, 738, 795, 856, 1100, 1224, 1391
TatI WGTACW 2 cut(s) 816, 1139
TfiI GAWTC 3 cut(s) 101, 122, 1164
Tru1I TTAA 2 cut(s) 524, 741
Tru9I TTAA 2 cut(s) 524, 741
TscAI CASTG 6 cut(s) 224, 240, 873, 1011, 1149, 1259
TseFI GTSAC 3 cut(s) 282, 766, 1237
TseI GCWGC 3 cut(s) 401, 724, 1118
Tsp45I GTSAC 3 cut(s) 282, 766, 1237
TspDTI ATGAA 8 cut(s) 46, 362, 555, 845, 1222, 1312, 1367, 1404
TspGWI ACGGA 1 cut(s) 583
TspRI CASTG 6 cut(s) 224, 240, 873, 1011, 1149, 1259
Tth111I GACNNNGTC 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 672
XapI RAATTY 3 cut(s) 1100, 1224, 1391
XceI RCATGY 1 cut(s) 1350
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmnI GAANNNNTTC 1 cut(s) 1054
XspI CTAG 3 cut(s) 546, 885, 1077
Zsp2I ATGCAT 2 cut(s) 1352, 1363
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.