RchiOBHm_Chr5g0076571

WEB family protein At5g55860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0009427
Physical Location & Seq
Reverse (-)
82216243 .. 82216866
624 bp
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UTR
Exon/CDS
Intron
PRQ35122

Sequence Viewer

Length: 570 bp
ATGAATGCTGAAACTACTAAAGCCCAAGCACTTGTGGAGCTTGCAAAGGCCAAAGTAACTCATGAAGAGCTGAACAAGAAGCTGGCATCTCTTGGTGAATTTAAGGAATCCGCAATAAAGGCATCAGAAGCTGCAAAAAATCGGGCAAAGCAACTCGAAGAAGCAAACAATGGGAACCTAGCTGGAACTGATGGTGCTTGGAAGCAAGACTTGGAAACAGATAGAGCACAATACATGAATGTAATTACTGAACTTGATGCTGCAAAGCAAGAGTTGAGGAAAATCCGTCAGGACTGTGATGCATCCTTAGAAGCAAAAGTTACTGCATTCAAGCAGGCAGCAGAAGCTAAAGATGCAGCCAAAGCAAATGCGGAGAGAGTTAATGAGCTCTCAAAGGAAATTGCAGCTGTGCATGAATCAATTGGGCAAGTGAACCTTGCATCTGAGGAAGCCCAGCAAGAGAAAGCAAAGGTATTTTCCGAAAAGGATGTCCTGAGGCAAGCATATAAGGCCACGGCAGAAGAGTCTGCAAAGAAATTGCTCTCTCTACAAATACAGTTTGATCCATAA

Protein Analysis

189

Amino Acids

20.7

Weight (kDa)

5.46

Isoelectric Point (pI)

36.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WEMBL PF05701 2 - 168 9.1e-11 Weak chloroplast movement under blue light
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 111, 371
AclWI GGATC 1 cut(s) 557
AcsI RAATTY 1 cut(s) 98
AgsI TTSAA 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 194, 226
AluBI AGCT 8 cut(s) 40, 70, 82, 131, 182, 347, 388, 407
AluI AGCT 8 cut(s) 40, 70, 82, 131, 182, 347, 388, 407
Alw21I GWGCWC 2 cut(s) 229, 390
AlwI GGATC 1 cut(s) 557
AlwNI CAGNNNCTG 1 cut(s) 131
AoxI GGCC 2 cut(s) 48, 510
ApeKI GCWGC 5 cut(s) 131, 260, 338, 356, 404
ApoI RAATTY 1 cut(s) 98
ArsI GACNNNNNNTTYG 2 cut(s) 474, 506
AsuHPI GGTGA 1 cut(s) 107
AxyI CCTNAGG 1 cut(s) 494
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 2 cut(s) 229, 390
BbvI GCAGC 5 cut(s) 118, 247, 350, 368, 416
BccI CCATC 1 cut(s) 185
BceAI ACGGC 1 cut(s) 531
BfaI CTAG 1 cut(s) 179
BisI GCNGC 5 cut(s) 132, 261, 339, 357, 405
BlsI GCNGC 5 cut(s) 133, 262, 340, 358, 406
BmiI GGNNCC 1 cut(s) 176
BmsI GCATC 7 cut(s) 95, 131, 247, 289, 311, 343, 449
BsaJI CCNNGG 1 cut(s) 513
Bse21I CCTNAGG 1 cut(s) 494
BseDI CCNNGG 1 cut(s) 513
BseGI GGATG 2 cut(s) 302, 493
BseMII CTCAG 2 cut(s) 435, 485
BseXI GCAGC 5 cut(s) 118, 247, 350, 368, 416
BseYI CCCAGC 1 cut(s) 453
BshFI GGCC 2 cut(s) 50, 512
BsiHKAI GWGCWC 2 cut(s) 229, 390
BsmI GAATGC 2 cut(s) 10, 326
BsnI GGCC 2 cut(s) 50, 512
Bsp1286I GDGCHC 2 cut(s) 229, 390
Bsp143I GATC 1 cut(s) 562
BspACI CCGC 2 cut(s) 111, 371
BspANI GGCC 2 cut(s) 50, 512
BspCNI CTCAG 2 cut(s) 436, 486
BspHI TCATGA 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 176
BspPI GGATC 1 cut(s) 557
BspQI GCTCTTC 1 cut(s) 60
BssECI CCNNGG 1 cut(s) 513
BssMI GATC 1 cut(s) 562
Bst4CI ACNGT 2 cut(s) 296, 558
Bst6I CTCTTC 2 cut(s) 60, 516
BstC8I GCNNGC 4 cut(s) 42, 84, 336, 501
BstDEI CTNAG 3 cut(s) 307, 444, 494
BstDSI CCRYGG 1 cut(s) 513
BstF5I GGATG 2 cut(s) 302, 493
BstKTI GATC 1 cut(s) 565
BstMBI GATC 1 cut(s) 562
BstMWI GCNNNNNNNGC 6 cut(s) 119, 128, 344, 353, 362, 509
BstV1I GCAGC 5 cut(s) 118, 247, 350, 368, 416
Bsu36I CCTNAGG 1 cut(s) 494
BsuRI GGCC 2 cut(s) 50, 512
BtgI CCRYGG 1 cut(s) 513
BtsCI GGATG 2 cut(s) 302, 493
Cac8I GCNNGC 4 cut(s) 42, 84, 336, 501
CaiI CAGNNNCTG 1 cut(s) 131
CciI TCATGA 1 cut(s) 61
CviAII CATG 3 cut(s) 62, 235, 413
DdeI CTNAG 3 cut(s) 307, 444, 494
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
Eam1104I CTCTTC 2 cut(s) 60, 516
EarI CTCTTC 2 cut(s) 60, 516
Ecl136II GAGCTC 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 390
Eco53kI GAGCTC 1 cut(s) 388
Eco81I CCTNAGG 1 cut(s) 494
EcoICRI GAGCTC 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 390
FaeI CATG 3 cut(s) 65, 238, 416
FaiI YATR 6 cut(s) 63, 236, 414, 505, 507, 568
FalI AAGNNNNNCTT 4 cut(s) 194, 226, 420, 452
FatI CATG 3 cut(s) 61, 234, 412
Fnu4HI GCNGC 5 cut(s) 132, 261, 339, 357, 405
FokI GGATG 2 cut(s) 289, 500
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 5 cut(s) 132, 261, 339, 357, 405
FspBI CTAG 1 cut(s) 179
GluI GCNGC 5 cut(s) 132, 261, 339, 357, 405
GsaI CCCAGC 1 cut(s) 457
HaeIII GGCC 2 cut(s) 50, 512
Hin1II CATG 3 cut(s) 65, 238, 416
HinfI GANTC 3 cut(s) 107, 416, 524
HphI GGTGA 1 cut(s) 107
Hpy166II GTNNAC 1 cut(s) 433
Hpy188I TCNGA 3 cut(s) 127, 445, 481
Hpy188III TCNNGA 3 cut(s) 62, 290, 493
Hpy8I GTNNAC 1 cut(s) 433
HpyCH4III ACNGT 2 cut(s) 296, 558
HpyF10VI GCNNNNNNNGC 6 cut(s) 119, 128, 344, 353, 362, 509
HpyF3I CTNAG 3 cut(s) 307, 444, 494
Hsp92II CATG 3 cut(s) 65, 238, 416
Kzo9I GATC 1 cut(s) 562
LguI GCTCTTC 1 cut(s) 60
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 6 cut(s) 68, 168, 275, 320, 467, 506
Lsp1109I GCAGC 5 cut(s) 118, 247, 350, 368, 416
LweI GCATC 7 cut(s) 95, 131, 247, 289, 311, 343, 449
MaeI CTAG 1 cut(s) 179
MaeIII GTNAC 2 cut(s) 55, 319
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MboII GAAGA 3 cut(s) 77, 170, 533
MfeI CAATTG 1 cut(s) 420
MhlI GDGCHC 2 cut(s) 229, 390
MluCI AATT 5 cut(s) 98, 243, 399, 420, 536
MlyI GAGTC 1 cut(s) 533
MnlI CCTC 3 cut(s) 270, 439, 489
Mph1103I ATGCAT 1 cut(s) 304
MseI TTAA 2 cut(s) 102, 381
MspA1I CMGCKG 1 cut(s) 407
MunI CAATTG 1 cut(s) 420
Mva1269I GAATGC 2 cut(s) 10, 326
MwoI GCNNNNNNNGC 6 cut(s) 119, 128, 344, 353, 362, 509
NdeII GATC 1 cut(s) 562
NlaIII CATG 3 cut(s) 65, 238, 416
NlaIV GGNNCC 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 304
PagI TCATGA 1 cut(s) 61
PciSI GCTCTTC 1 cut(s) 60
PctI GAATGC 2 cut(s) 10, 326
PfeI GAWTC 2 cut(s) 107, 416
PkrI GCNGC 5 cut(s) 133, 262, 340, 358, 406
PleI GAGTC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 532
Psp124BI GAGCTC 1 cut(s) 390
PspFI CCCAGC 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 176
PstNI CAGNNNCTG 1 cut(s) 131
PvuII CAGCTG 1 cut(s) 407
SacI GAGCTC 1 cut(s) 390
SapI GCTCTTC 1 cut(s) 60
SaqAI TTAA 2 cut(s) 102, 381
SatI GCNGC 5 cut(s) 132, 261, 339, 357, 405
Sau3AI GATC 1 cut(s) 562
SchI GAGTC 1 cut(s) 533
SduI GDGCHC 2 cut(s) 229, 390
SfaNI GCATC 7 cut(s) 95, 131, 247, 289, 311, 343, 449
Sse9I AATT 5 cut(s) 98, 243, 399, 420, 536
SsiI CCGC 2 cut(s) 111, 371
SspMI CTAG 1 cut(s) 179
SstI GAGCTC 1 cut(s) 390
TaaI ACNGT 2 cut(s) 296, 558
TaqI TCGA 1 cut(s) 156
TasI AATT 5 cut(s) 98, 243, 399, 420, 536
TfiI GAWTC 2 cut(s) 107, 416
Tru1I TTAA 2 cut(s) 102, 381
Tru9I TTAA 2 cut(s) 102, 381
TseI GCWGC 5 cut(s) 131, 260, 338, 356, 404
TspDTI ATGAA 4 cut(s) 17, 78, 251, 429
TspGWI ACGGA 1 cut(s) 275
XapI RAATTY 1 cut(s) 98
XspI CTAG 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.