Rorug05G0448500

WEB family protein At5g55860-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009427
Physical Location & Seq
Reverse (-)
62038888 .. 62039478
591 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0448500.1

Sequence Viewer

Length: 591 bp
ATGACATCCCCAGGTTCTCGTCCTCCGGATCCGTGGCTCATTGCTTCTGTTGCTATCCTCTGCACTTTTACTGTCATCTTCAGTTATTGCAAGATACTGAAACGGTGCAATACAAGATCCAATCCAAACCACAGACGTACCCTTCTGAACGAGACCAACGCAGATGATCCATCTCTGCAGTTTCAAAGCCCAGGACTAGATTTCGCAATCATCCGGTCTCTACCCATAACTCAGTTTAAGAAGAGAAGTGTAGCAGAAGATGCAGAAGAGCACCAAAGCAACACAGACTGTCCAGTATGCTTGGGTGAATTTGAAGAAGGGGAATGGCTAAGACATCTACCCAAATGCAATCATGCATTCCACATATCTTGCATCGACACTTGGTTTGTGGCTCACTCAAATTGCCCCTTATGCAGATCAGAAGTCTATGATTTTATCCAGGATTGTCCTATTCCTATGCACACTCTGTTAGAGACACTGAGGGAAAGTGTAATTCAAGAAAGAGCGTTACACTTCCAAATGCTGCGCTCTGCGGTCTTGGAGAACTCACCATTTGGACGTGAAGCAAGATTCGGCGACGGCAACGTGTAG

Protein Analysis

196

Amino Acids

22.35

Weight (kDa)

5.89

Isoelectric Point (pI)

65.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 96 - 139 3.9e-13 Ring finger domain
zf-RING_11 PF17123 96 - 125 6.6e-09 RING-like zinc finger
zf-rbx1 PF12678 96 - 139 3.8e-06 RING-H2 zinc finger domain
zf-C3HC4_2 PF13923 97 - 138 6.6e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 97 - 138 1.5e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 25
AciI CCGC 1 cut(s) 533
AclWI GGATC 4 cut(s) 23, 36, 111, 161
AcsI RAATTY 1 cut(s) 308
AcuI CTGAAG 1 cut(s) 64
AfaI GTAC 1 cut(s) 139
AflIII ACRYGT 1 cut(s) 585
AgsI TTSAA 3 cut(s) 185, 314, 497
AjiI CACGTC 1 cut(s) 560
AjnI CCWGG 3 cut(s) 10, 190, 438
Alw21I GWGCWC 1 cut(s) 273
Alw26I GTCTC 3 cut(s) 146, 222, 467
AlwI GGATC 4 cut(s) 23, 36, 111, 161
Aor13HI TCCGGA 1 cut(s) 25
ApeKI GCWGC 1 cut(s) 523
ApoI RAATTY 1 cut(s) 308
ArsI GACNNNNNNTTYG 2 cut(s) 368, 400
AspLEI GCGC 1 cut(s) 528
AsuHPI GGTGA 2 cut(s) 317, 540
BaeI ACNNNNGTAYC 2 cut(s) 121, 154
BamHI GGATCC 1 cut(s) 28
Bbv12I GWGCWC 1 cut(s) 273
BbvI GCAGC 1 cut(s) 510
BccI CCATC 1 cut(s) 178
BciT130I CCWGG 3 cut(s) 12, 192, 440
BcoDI GTCTC 3 cut(s) 146, 222, 467
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 1 cut(s) 176
BisI GCNGC 1 cut(s) 524
BlsI GCNGC 1 cut(s) 525
Bme1390I CCNGG 3 cut(s) 12, 192, 440
BmgBI CACGTC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 30
BmrFI CCNGG 3 cut(s) 12, 192, 440
BmsI GCATC 2 cut(s) 250, 381
BsaI GGTCTC 2 cut(s) 146, 222
BsaJI CCNNGG 3 cut(s) 10, 32, 190
BsaWI WCCGGW 2 cut(s) 25, 213
Bse1I ACTGG 1 cut(s) 293
Bse3DI GCAATG 1 cut(s) 39
BseAI TCCGGA 1 cut(s) 25
BseBI CCWGG 3 cut(s) 12, 192, 440
BseDI CCNNGG 3 cut(s) 10, 32, 190
BseGI GGATG 2 cut(s) 5, 210
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 245, 470
BseNI ACTGG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 510
BsgI GTGCAG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 273
BsiSI CCGG 2 cut(s) 26, 214
BsmAI GTCTC 3 cut(s) 146, 222, 467
BsmI GAATGC 1 cut(s) 356
Bso31I GGTCTC 2 cut(s) 146, 222
Bsp1286I GDGCHC 1 cut(s) 273
Bsp13I TCCGGA 1 cut(s) 25
Bsp143I GATC 4 cut(s) 28, 116, 166, 416
BspACI CCGC 1 cut(s) 533
BspCNI CTCAG 2 cut(s) 244, 471
BspEI TCCGGA 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 30
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 4 cut(s) 23, 36, 111, 161
BspQI GCTCTTC 1 cut(s) 261
BspTNI GGTCTC 2 cut(s) 146, 222
BsrDI GCAATG 1 cut(s) 39
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 10, 32, 190
BssMI GATC 4 cut(s) 28, 116, 166, 416
Bst2UI CCWGG 3 cut(s) 12, 192, 440
Bst4CI ACNGT 3 cut(s) 73, 105, 290
Bst6I CTCTTC 2 cut(s) 236, 261
BstAPI GCANNNNNTGC 1 cut(s) 260
BstDEI CTNAG 3 cut(s) 231, 329, 479
BstDSI CCRYGG 1 cut(s) 32
BstF5I GGATG 2 cut(s) 5, 210
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 4 cut(s) 31, 119, 169, 419
BstMAI GTCTC 3 cut(s) 146, 222, 467
BstMBI GATC 4 cut(s) 28, 116, 166, 416
BstMWI GCNNNNNNNGC 3 cut(s) 50, 260, 411
BstNI CCWGG 3 cut(s) 12, 192, 440
BstSCI CCNGG 3 cut(s) 10, 190, 438
BstSFI CTRYAG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 510
BstX2I RGATCY 2 cut(s) 28, 116
BstYI RGATCY 2 cut(s) 28, 116
BtgI CCRYGG 1 cut(s) 32
BtrI CACGTC 1 cut(s) 560
BtsCI GGATG 2 cut(s) 5, 210
BtsIMutI CAGTG 1 cut(s) 476
CfoI GCGC 1 cut(s) 528
Csp6I GTAC 1 cut(s) 138
CspCI CAANNNNNGTGG 2 cut(s) 350, 385
CviAII CATG 1 cut(s) 353
CviJI RGCY 4 cut(s) 37, 189, 328, 392
CviKI_1 RGCY 4 cut(s) 37, 189, 328, 392
CviQI GTAC 1 cut(s) 138
DdeI CTNAG 3 cut(s) 231, 329, 479
DpnI GATC 4 cut(s) 30, 118, 168, 418
DpnII GATC 4 cut(s) 28, 116, 166, 416
Eam1104I CTCTTC 2 cut(s) 236, 261
EarI CTCTTC 2 cut(s) 236, 261
Eco31I GGTCTC 2 cut(s) 146, 222
Eco57I CTGAAG 1 cut(s) 64
EcoRII CCWGG 3 cut(s) 10, 190, 438
EcoT22I ATGCAT 1 cut(s) 358
FaeI CATG 1 cut(s) 356
FaiI YATR 7 cut(s) 227, 298, 354, 365, 412, 429, 458
FatI CATG 1 cut(s) 352
Fnu4HI GCNGC 1 cut(s) 524
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 1 cut(s) 524
FspBI CTAG 1 cut(s) 197
GlaI GCGC 1 cut(s) 527
GluI GCNGC 1 cut(s) 524
HapII CCGG 2 cut(s) 26, 214
HhaI GCGC 1 cut(s) 528
Hin1II CATG 1 cut(s) 356
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HinfI GANTC 1 cut(s) 570
HpaII CCGG 2 cut(s) 26, 214
HphI GGTGA 2 cut(s) 317, 540
Hpy188I TCNGA 2 cut(s) 147, 421
Hpy188III TCNNGA 2 cut(s) 26, 497
Hpy99I CGWCG 1 cut(s) 581
HpyAV CCTTC 2 cut(s) 152, 311
HpyCH4III ACNGT 3 cut(s) 73, 105, 290
HpyCH4IV ACGT 3 cut(s) 136, 559, 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 50, 260, 411
HpyF3I CTNAG 3 cut(s) 231, 329, 479
HpySE526I ACGT 3 cut(s) 136, 559, 585
Hsp92II CATG 1 cut(s) 356
HspAI GCGC 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 25
Kzo9I GATC 4 cut(s) 28, 116, 166, 416
LguI GCTCTTC 1 cut(s) 261
LpnPI CCDG 8 cut(s) 24, 39, 177, 204, 227, 306, 425, 452
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 2 cut(s) 250, 381
MaeI CTAG 1 cut(s) 197
MaeII ACGT 3 cut(s) 136, 559, 585
MaeIII GTNAC 1 cut(s) 507
MalI GATC 4 cut(s) 30, 118, 168, 418
MboI GATC 4 cut(s) 28, 116, 166, 416
MboII GAAGA 5 cut(s) 70, 253, 269, 278, 326
MflI RGATCY 2 cut(s) 28, 116
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 3 cut(s) 308, 400, 492
MnlI CCTC 3 cut(s) 33, 68, 474
Mph1103I ATGCAT 1 cut(s) 358
MroI TCCGGA 1 cut(s) 25
MseI TTAA 1 cut(s) 237
MspI CCGG 2 cut(s) 26, 214
MspR9I CCNGG 3 cut(s) 12, 192, 440
Mva1269I GAATGC 1 cut(s) 356
MvaI CCWGG 3 cut(s) 12, 192, 440
MwoI GCNNNNNNNGC 3 cut(s) 50, 260, 411
NdeII GATC 4 cut(s) 28, 116, 166, 416
NlaIII CATG 1 cut(s) 356
NlaIV GGNNCC 1 cut(s) 30
NsiI ATGCAT 1 cut(s) 358
PciSI GCTCTTC 1 cut(s) 261
PctI GAATGC 1 cut(s) 356
PfeI GAWTC 1 cut(s) 570
PfoI TCCNGGA 1 cut(s) 438
PkrI GCNGC 1 cut(s) 525
Psp6I CCWGG 3 cut(s) 10, 190, 438
PspGI CCWGG 3 cut(s) 10, 190, 438
PspN4I GGNNCC 1 cut(s) 30
PstI CTGCAG 1 cut(s) 180
PsuI RGATCY 2 cut(s) 28, 116
RsaI GTAC 1 cut(s) 139
RsaNI GTAC 1 cut(s) 138
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 1 cut(s) 524
Sau3AI GATC 4 cut(s) 28, 116, 166, 416
ScrFI CCNGG 3 cut(s) 12, 192, 440
SduI GDGCHC 1 cut(s) 273
SetI ASST 4 cut(s) 16, 139, 562, 588
SfaNI GCATC 2 cut(s) 250, 381
SfcI CTRYAG 1 cut(s) 176
Sse9I AATT 3 cut(s) 308, 400, 492
SsiI CCGC 1 cut(s) 533
SspMI CTAG 1 cut(s) 197
StyD4I CCNGG 3 cut(s) 10, 190, 438
TaaI ACNGT 3 cut(s) 73, 105, 290
TaiI ACGT 3 cut(s) 139, 562, 588
TaqI TCGA 1 cut(s) 375
TasI AATT 3 cut(s) 308, 400, 492
TfiI GAWTC 1 cut(s) 570
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TscAI CASTG 1 cut(s) 483
TseI GCWGC 1 cut(s) 523
TspGWI ACGGA 1 cut(s) 21
TspRI CASTG 1 cut(s) 483
XapI RAATTY 1 cut(s) 308
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.