RchiOBHm_Chr6g0289001

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003462
Physical Location & Seq
Reverse (-)
52215566 .. 52217387
1822 bp
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UTR
Exon/CDS
Intron
PRQ25928

Sequence Viewer

Length: 1509 bp
ATGTTTCTCGAGCTGTTAGCCTTCTCCCCCTTTCTCCTCCTCCCCTTGCTTTTCTTCATCCTCAGGCTTTTCGGCAACTCGAAAACCAACGGCAACAGCAAGCTCCCCAAATCATACCCAATAATCGGTTCTTTCCTAGCCCTCTACAAGAACTATGCCAATAGACTCCTATGGTTCACTGAGCTTCTCCAAGACTCGCCCACATCTACTATTACCATGCACCATTCTCTGGGCCGCCACTTTGTGGTCACCGCCAACCCGACTGTGGTCCAACACATCCTCAAGACAAAGTTCCACATCTACCAAAAGGGCGAGTCCTTCCGCACAACTCTCCACGACCTCTTCGGCGACGGCCTTTTCAACGCCGACGGTGACAATTGGAAGTTCCAGCGGCAGCTCTCTAGCCATGAATTCAACACAAAGTCGCTCAGGAAATTCGTGGAAGAGGTGGTGGACACTGAGCTGTACAATCGGCTGATTCCCATTCTATCCACATCTTCCAGCCAACGAACCGTTCTCGATTTCCAAGACATTCTCCAGCGGTTCGCCTTCGACAACATTTGCCGGATCGGGTTCGGTTACGACCCGGAGTATCTCCTTCCTTCTCTGCCGCAAGCTAAATTCGCTGTGGCGTTCGACGATGCGGTCCAGATCAGTGAAGAAAGGTTCAATTCCATGCGCCCGGTGTGGAAGATCAAACGAGCTCTCGGAATCGGATCGGAAAGCCGTCTCCACCGTGCCGTCTCCGACGTCCGAGAGTTTGCGAAGACCATCGTCAGGGAAAAGAAGCGCGAACTAGACGAGAAGAAGACTCTAGAGTCTCACGATTTGTTGTCTCGATTCTTGAACTCCGGCCACTCCGAAGAGAACTTTGTCACCGACATCGTCATCACCTTCATCCTCGCCGGAAAGGATACGACCTCGGCTGCTCTGACCTGGTTCTTCTGGCTAATCTCAAACCATCCCAACGTCGAAAGCCGAATTCTCAAGGAGATCAATGAGAAAACGGAGAGCGAAGCTGGATATGACGAAGCCAAGGACATGGTGTACACACAAGCGGCGCTCTGCGAGAGCATGAGGCTGTACCCGCCAGTTCCGAGGGACGTCAAAGAGGCGGTAGACGACGACGTTTTGCCGGACGGGACTAAAGTGAAGAAGGGGATGACAGTGGTTTACCACATATACGCGATGGGGAGGATGGAGCAGATATGGGGCAAGGATTGGGCCGAGTTCCGACCCGAGAGGTGGTTGGAGCATGACATGGCGACGGAGAATTGGCGGTTCGTGGCGAAGGACTCATTTAGCTACCCGGTGTTTCAGGCGGGGCCGAGGATCTGTTTGGGGAAGGAGATGGCATTTTTGCAGATGAAAAGGGTGGTGGGTGAGGTTTTGAAGAGGTTTAAGGTTGTACCGGAGAAGAGGGAGAATGGGAAGGAGCCGGATATGCTTACCTACCTTACCGTAAAAATGAAAGGTGGGTTTCCGGTGACCGTTGTGGAGAGGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

502

Amino Acids

58.06

Weight (kDa)

7.72

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 35 - 483 3.2e-66 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 644, 817
AatII GACGTC 2 cut(s) 753, 1107
AccB7I CCANNNNNTGG 2 cut(s) 229, 244
AccI GTMKAC 1 cut(s) 1119
AccII CGCG 2 cut(s) 792, 1187
AclWI GGATC 3 cut(s) 575, 724, 1340
AcoI YGGCCR 1 cut(s) 853
AcsI RAATTY 4 cut(s) 410, 434, 620, 981
AcyI GRCGYC 2 cut(s) 750, 1104
AdeI CACNNNGTG 1 cut(s) 244
AfaI GTAC 4 cut(s) 467, 1049, 1085, 1410
AfiI CCNNNNNNNGG 6 cut(s) 125, 229, 244, 265, 777, 1245
AgsI TTSAA 5 cut(s) 361, 415, 670, 847, 1393
AjnI CCWGG 1 cut(s) 935
AjuI GAANNNNNNNTTGG 4 cut(s) 112, 144, 498, 530
AluBI AGCT 9 cut(s) 13, 103, 184, 397, 463, 617, 704, 1019, 1305
AluI AGCT 9 cut(s) 13, 103, 184, 397, 463, 617, 704, 1019, 1305
Alw21I GWGCWC 1 cut(s) 706
Alw26I GTCTC 4 cut(s) 734, 748, 825, 840
AlwI GGATC 3 cut(s) 575, 724, 1340
Ama87I CYCGRG 2 cut(s) 8, 1238
AoxI GGCC 5 cut(s) 232, 352, 853, 1224, 1325
ApeKI GCWGC 2 cut(s) 394, 926
ApoI RAATTY 4 cut(s) 410, 434, 620, 981
AspLEI GCGC 3 cut(s) 681, 792, 1063
AspS9I GGNCC 5 cut(s) 232, 268, 646, 1224, 1325
AsuC2I CCSGG 3 cut(s) 587, 683, 1310
AsuHPI GGTGA 6 cut(s) 241, 383, 868, 883, 1394, 1498
AvaI CYCGRG 2 cut(s) 8, 1238
AvaII GGWCC 2 cut(s) 268, 646
AxyI CCTNAGG 1 cut(s) 62
BanII GRGCYC 1 cut(s) 706
BbsI GAAGAC 2 cut(s) 773, 815
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 2 cut(s) 406, 913
BccI CCATC 5 cut(s) 779, 969, 1183, 1192, 1345
BceAI ACGGC 4 cut(s) 106, 367, 711, 725
BciT130I CCWGG 1 cut(s) 937
BciVI GTATCC 1 cut(s) 907
BcnI CCSGG 3 cut(s) 587, 683, 1310
BcoDI GTCTC 4 cut(s) 734, 748, 825, 840
BfaI CTAG 4 cut(s) 137, 402, 797, 815
BfoI RGCGCY 1 cut(s) 1064
BfuI GTATCC 1 cut(s) 907
BisI GCNGC 6 cut(s) 235, 392, 395, 611, 927, 1059
BlsI GCNGC 6 cut(s) 236, 393, 396, 612, 928, 1060
Bme1390I CCNGG 4 cut(s) 587, 683, 937, 1310
Bme18I GGWCC 2 cut(s) 268, 646
BmeT110I CYCGRG 2 cut(s) 8, 1238
BmgT120I GGNCC 5 cut(s) 232, 268, 646, 1224, 1325
BmiI GGNNCC 2 cut(s) 1326, 1437
BmrFI CCNGG 4 cut(s) 587, 683, 937, 1310
BmsI GCATC 1 cut(s) 631
BoxI GACNNNNGTC 2 cut(s) 266, 773
BpiI GAAGAC 2 cut(s) 773, 815
BpmI CTGGAG 1 cut(s) 521
Bpu10I CCTNAGC 1 cut(s) 428
BpuEI CTTGAG 2 cut(s) 266, 971
BpuMI CCSGG 3 cut(s) 587, 683, 1310
BsaHI GRCGYC 2 cut(s) 750, 1104
BsaJI CCNNGG 4 cut(s) 921, 1035, 1097, 1328
BsaWI WCCGGW 2 cut(s) 1411, 1483
Bsc4I CCNNNNNNNGG 6 cut(s) 125, 229, 244, 265, 777, 1245
Bse1I ACTGG 1 cut(s) 1091
Bse21I CCTNAGG 1 cut(s) 62
BseBI CCWGG 1 cut(s) 937
BseDI CCNNGG 4 cut(s) 921, 1035, 1097, 1328
BseGI GGATG 6 cut(s) 57, 276, 897, 961, 1167, 1203
BseLI CCNNNNNNNGG 6 cut(s) 125, 229, 244, 265, 777, 1245
BseMII CTCAG 4 cut(s) 76, 171, 442, 450
BseNI ACTGG 1 cut(s) 1091
BseRI GAGGAG 2 cut(s) 26, 29
BseXI GCAGC 2 cut(s) 406, 913
Bsh1236I CGCG 2 cut(s) 792, 1187
BshFI GGCC 5 cut(s) 234, 354, 855, 1226, 1327
BsiHKAI GWGCWC 1 cut(s) 706
BsiHKCI CYCGRG 2 cut(s) 8, 1238
BslFI GGGAC 2 cut(s) 1115, 1156
BslI CCNNNNNNNGG 6 cut(s) 125, 229, 244, 265, 777, 1245
BsmAI GTCTC 4 cut(s) 734, 748, 825, 840
BsmBI CGTCTC 2 cut(s) 734, 748
BsmFI GGGAC 2 cut(s) 1115, 1156
BsnI GGCC 5 cut(s) 234, 354, 855, 1226, 1327
BsoBI CYCGRG 2 cut(s) 8, 1238
Bsp1286I GDGCHC 1 cut(s) 706
Bsp1407I TGTACA 2 cut(s) 465, 1047
Bsp143I GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
BspANI GGCC 5 cut(s) 234, 354, 855, 1226, 1327
BspCNI CTCAG 4 cut(s) 75, 172, 441, 451
BspFNI CGCG 2 cut(s) 792, 1187
BspLI GGNNCC 2 cut(s) 1326, 1437
BspPI GGATC 3 cut(s) 575, 724, 1340
BsrGI TGTACA 2 cut(s) 465, 1047
BsrI ACTGG 1 cut(s) 1091
BssECI CCNNGG 4 cut(s) 921, 1035, 1097, 1328
BssMI GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
BssNI GRCGYC 2 cut(s) 750, 1104
BssT1I CCWWGG 1 cut(s) 1035
Bst2UI CCWGG 1 cut(s) 937
Bst4CI ACNGT 7 cut(s) 265, 371, 514, 737, 1168, 1462, 1492
Bst6I CTCTTC 5 cut(s) 347, 438, 858, 1388, 1412
BstACI GRCGYC 2 cut(s) 750, 1104
BstAUI TGTACA 2 cut(s) 465, 1047
BstC8I GCNNGC 2 cut(s) 101, 615
BstDEI CTNAG 4 cut(s) 62, 180, 428, 459
BstEII GGTNACC 2 cut(s) 247, 1486
BstF5I GGATG 6 cut(s) 57, 276, 897, 961, 1167, 1203
BstFNI CGCG 2 cut(s) 792, 1187
BstH2I RGCGCY 1 cut(s) 1064
BstHHI GCGC 3 cut(s) 681, 792, 1063
BstKTI GATC 6 cut(s) 570, 654, 696, 719, 996, 1335
BstMAI GTCTC 4 cut(s) 734, 748, 825, 840
BstMBI GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
BstMWI GCNNNNNNNGC 3 cut(s) 623, 1087, 1444
BstNI CCWGG 1 cut(s) 937
BstPAI GACNNNNGTC 2 cut(s) 266, 773
BstPI GGTNACC 2 cut(s) 247, 1486
BstSCI CCNGG 4 cut(s) 585, 681, 935, 1308
BstUI CGCG 2 cut(s) 792, 1187
BstV1I GCAGC 2 cut(s) 406, 913
BstV2I GAAGAC 2 cut(s) 773, 815
BstX2I RGATCY 1 cut(s) 1332
BstXI CCANNNNNNTGG 1 cut(s) 1042
BstYI RGATCY 1 cut(s) 1332
Bsu36I CCTNAGG 1 cut(s) 62
BsuI GTATCC 1 cut(s) 907
BsuRI GGCC 5 cut(s) 234, 354, 855, 1226, 1327
BtgZI GCGATG 1 cut(s) 1202
BtsCI GGATG 6 cut(s) 57, 276, 897, 961, 1167, 1203
BtsIMutI CAGTG 4 cut(s) 177, 456, 661, 1173
Cac8I GCNNGC 2 cut(s) 101, 615
CfoI GCGC 3 cut(s) 681, 792, 1063
Cfr13I GGNCC 5 cut(s) 232, 268, 646, 1224, 1325
CsiI ACCWGGT 1 cut(s) 935
Csp6I GTAC 4 cut(s) 466, 1048, 1084, 1409
CviAII CATG 7 cut(s) 217, 407, 676, 1042, 1075, 1256, 1261
CviQI GTAC 4 cut(s) 466, 1048, 1084, 1409
DdeI CTNAG 4 cut(s) 62, 180, 428, 459
DpnI GATC 6 cut(s) 569, 653, 695, 718, 995, 1334
DpnII GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
DraIII CACNNNGTG 1 cut(s) 244
DrdI GACNNNNNNGTC 2 cut(s) 644, 817
DseDI GACNNNNNNGTC 2 cut(s) 644, 817
EaeI YGGCCR 1 cut(s) 853
Eam1104I CTCTTC 5 cut(s) 347, 438, 858, 1388, 1412
EarI CTCTTC 5 cut(s) 347, 438, 858, 1388, 1412
Ecl136II GAGCTC 1 cut(s) 704
Eco130I CCWWGG 1 cut(s) 1035
Eco24I GRGCYC 1 cut(s) 706
Eco47I GGWCC 2 cut(s) 268, 646
Eco53kI GAGCTC 1 cut(s) 704
Eco81I CCTNAGG 1 cut(s) 62
Eco88I CYCGRG 2 cut(s) 8, 1238
Eco91I GGTNACC 2 cut(s) 247, 1486
EcoICRI GAGCTC 1 cut(s) 704
EcoO65I GGTNACC 2 cut(s) 247, 1486
EcoRI GAATTC 2 cut(s) 410, 981
EcoRII CCWGG 1 cut(s) 935
EcoT14I CCWWGG 1 cut(s) 1035
EcoT38I GRGCYC 1 cut(s) 706
ErhI CCWWGG 1 cut(s) 1035
Esp3I CGTCTC 2 cut(s) 734, 748
FaeI CATG 7 cut(s) 220, 410, 679, 1045, 1078, 1259, 1264
FaqI GGGAC 2 cut(s) 1115, 1156
FatI CATG 7 cut(s) 216, 406, 675, 1041, 1074, 1255, 1260
FauI CCCGC 2 cut(s) 1095, 1315
FblI GTMKAC 1 cut(s) 1119
Fnu4HI GCNGC 6 cut(s) 235, 392, 395, 611, 927, 1059
FokI GGATG 6 cut(s) 44, 263, 884, 948, 1174, 1210
FriOI GRGCYC 1 cut(s) 706
Fsp4HI GCNGC 6 cut(s) 235, 392, 395, 611, 927, 1059
FspBI CTAG 4 cut(s) 137, 402, 797, 815
GlaI GCGC 3 cut(s) 680, 791, 1062
GluI GCNGC 6 cut(s) 235, 392, 395, 611, 927, 1059
GsuI CTGGAG 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 1064
HaeIII GGCC 5 cut(s) 234, 354, 855, 1226, 1327
HhaI GCGC 3 cut(s) 681, 792, 1063
Hin1I GRCGYC 2 cut(s) 750, 1104
Hin1II CATG 7 cut(s) 220, 410, 679, 1045, 1078, 1259, 1264
Hin6I GCGC 3 cut(s) 679, 790, 1061
HinP1I GCGC 3 cut(s) 679, 790, 1061
HinfI GANTC 9 cut(s) 165, 194, 314, 478, 711, 811, 818, 840, 1295
HphI GGTGA 6 cut(s) 241, 383, 868, 883, 1394, 1498
Hpy166II GTNNAC 6 cut(s) 177, 454, 1048, 1050, 1120, 1174
Hpy188I TCNGA 9 cut(s) 710, 716, 721, 748, 755, 862, 933, 1098, 1235
Hpy188III TCNNGA 9 cut(s) 8, 283, 430, 518, 649, 815, 824, 837, 844
Hpy8I GTNNAC 6 cut(s) 177, 454, 1048, 1050, 1120, 1174
Hpy99I CGWCG 8 cut(s) 353, 371, 641, 752, 974, 1127, 1130, 1270
HpyCH4III ACNGT 7 cut(s) 265, 371, 514, 737, 1168, 1462, 1492
HpyCH4IV ACGT 4 cut(s) 750, 969, 1104, 1128
HpyCH4V TGCA 2 cut(s) 220, 1363
HpyF10VI GCNNNNNNNGC 3 cut(s) 623, 1087, 1444
HpyF3I CTNAG 4 cut(s) 62, 180, 428, 459
HpySE526I ACGT 4 cut(s) 750, 969, 1104, 1128
Hsp92I GRCGYC 2 cut(s) 750, 1104
Hsp92II CATG 7 cut(s) 220, 410, 679, 1045, 1078, 1259, 1264
HspAI GCGC 3 cut(s) 679, 790, 1061
Kzo9I GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
LmnI GCTCC 4 cut(s) 108, 1201, 1252, 1435
Lsp1109I GCAGC 2 cut(s) 406, 913
LweI GCATC 1 cut(s) 631
MabI ACCWGGT 1 cut(s) 935
MaeI CTAG 4 cut(s) 137, 402, 797, 815
MaeII ACGT 4 cut(s) 750, 969, 1104, 1128
MaeIII GTNAC 5 cut(s) 247, 371, 578, 874, 1486
MalI GATC 6 cut(s) 569, 653, 695, 718, 995, 1334
MboI GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
MfeI CAATTG 1 cut(s) 376
MflI RGATCY 1 cut(s) 1332
MhlI GDGCHC 1 cut(s) 706
MluCI AATT 7 cut(s) 376, 410, 434, 620, 670, 981, 1273
MlyI GAGTC 6 cut(s) 159, 188, 323, 805, 827, 1289
MmeI TCCRAC 4 cut(s) 295, 771, 1230, 1258
MseI TTAA 1 cut(s) 1401
MspA1I CMGCKG 2 cut(s) 391, 541
MspR9I CCNGG 4 cut(s) 587, 683, 937, 1310
MunI CAATTG 1 cut(s) 376
MvaI CCWGG 1 cut(s) 937
MvnI CGCG 2 cut(s) 792, 1187
MwoI GCNNNNNNNGC 3 cut(s) 623, 1087, 1444
NciI CCSGG 3 cut(s) 587, 683, 1310
NdeII GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
NlaIII CATG 7 cut(s) 220, 410, 679, 1045, 1078, 1259, 1264
NlaIV GGNNCC 2 cut(s) 1326, 1437
NmeAIII GCCGAG 3 cut(s) 902, 1252, 1353
NmuCI GTSAC 4 cut(s) 247, 371, 874, 1486
PaeR7I CTCGAG 1 cut(s) 8
PfeI GAWTC 3 cut(s) 478, 711, 840
PflFI GACNNNGTC 1 cut(s) 884
PflMI CCANNNNNTGG 2 cut(s) 229, 244
PkrI GCNGC 6 cut(s) 236, 393, 396, 612, 928, 1060
PleI GAGTC 6 cut(s) 159, 188, 322, 805, 826, 1289
PpsI GAGTC 6 cut(s) 159, 188, 322, 805, 826, 1289
PshAI GACNNNNGTC 2 cut(s) 266, 773
Psp124BI GAGCTC 1 cut(s) 706
Psp6I CCWGG 1 cut(s) 935
PspEI GGTNACC 2 cut(s) 247, 1486
PspGI CCWGG 1 cut(s) 935
PspN4I GGNNCC 2 cut(s) 1326, 1437
PspPI GGNCC 5 cut(s) 232, 268, 646, 1224, 1325
PsuI RGATCY 1 cut(s) 1332
PsyI GACNNNGTC 1 cut(s) 884
RsaI GTAC 4 cut(s) 467, 1049, 1085, 1410
RsaNI GTAC 4 cut(s) 466, 1048, 1084, 1409
SacI GAGCTC 1 cut(s) 706
SaqAI TTAA 1 cut(s) 1401
SatI GCNGC 6 cut(s) 235, 392, 395, 611, 927, 1059
Sau3AI GATC 6 cut(s) 567, 651, 693, 716, 993, 1332
Sau96I GGNCC 5 cut(s) 232, 268, 646, 1224, 1325
SchI GAGTC 6 cut(s) 159, 188, 323, 805, 827, 1289
ScrFI CCNGG 4 cut(s) 587, 683, 937, 1310
SduI GDGCHC 1 cut(s) 706
SexAI ACCWGGT 1 cut(s) 935
SfaNI GCATC 1 cut(s) 631
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 2 cut(s) 268, 646
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 281, 986
SmoI CTYRAG 3 cut(s) 8, 281, 986
Sse9I AATT 7 cut(s) 376, 410, 434, 620, 670, 981, 1273
SspMI CTAG 4 cut(s) 137, 402, 797, 815
SstI GAGCTC 1 cut(s) 706
StyD4I CCNGG 4 cut(s) 585, 681, 935, 1308
StyI CCWWGG 1 cut(s) 1035
TaaI ACNGT 7 cut(s) 265, 371, 514, 737, 1168, 1462, 1492
TaiI ACGT 4 cut(s) 753, 972, 1107, 1131
TaqI TCGA 7 cut(s) 9, 80, 519, 552, 636, 838, 972
TasI AATT 7 cut(s) 376, 410, 434, 620, 670, 981, 1273
TatI WGTACW 2 cut(s) 465, 1047
TauI GCSGC 4 cut(s) 237, 394, 613, 1061
TfiI GAWTC 3 cut(s) 478, 711, 840
Tru1I TTAA 1 cut(s) 1401
Tru9I TTAA 1 cut(s) 1401
TscAI CASTG 4 cut(s) 184, 463, 661, 1173
TseFI GTSAC 4 cut(s) 247, 371, 874, 1486
TseI GCWGC 2 cut(s) 394, 926
Tsp45I GTSAC 4 cut(s) 247, 371, 874, 1486
TspDTI ATGAA 5 cut(s) 46, 423, 886, 1382, 1484
TspGWI ACGGA 2 cut(s) 1022, 1283
TspRI CASTG 4 cut(s) 184, 463, 661, 1173
Tth111I GACNNNGTC 1 cut(s) 884
Van91I CCANNNNNTGG 2 cut(s) 229, 244
VpaK11BI GGWCC 2 cut(s) 268, 646
XapI RAATTY 4 cut(s) 410, 434, 620, 981
XbaI TCTAGA 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 262
XhoI CTCGAG 1 cut(s) 8
XmiI GTMKAC 1 cut(s) 1119
XspI CTAG 4 cut(s) 137, 402, 797, 815
ZraI GACGTC 2 cut(s) 751, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.