Rw6G026950

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003462
Physical Location & Seq
Reverse (-)
50665416 .. 50678025
12610 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026950.1

Sequence Viewer

Length: 1389 bp
ATGTTTCTCGAGCTACTAGCCTTAGCCCTTTTCTTGCTCCCTTTGCTTTTCCTCATTCTCAAGTTTTCCGGCAACCCGGAAACCGGCAACAGCAAGCTCCCAAAATCATACCCAATAATCGGTTCATTCCCAGCCCTCTACAAGAACCATGCCAACAGACTCGCATGGTTTCTTCTCCAGAACTCTCCCTCGGCCACTATCTCTATTCACCACTCTTTCGGCCAACACTCCGTCGTCACCGCCAACCCCACTGTGGTTCAACACATCCTCAAGACAAACTTCCCCACCTACCAAAAAGGTGAGCAACTCTCCAGCCACGAATTCAACACCAAGTCACTGAGAAAATTCGTTGAAGACGTGGTTGACATTGAACTCTACGACAGGCTCGTACCCATTCTATCCAAATCTTCTAGCGAGCGAACCATTCTTGATTTCCAAGACATTCTCCAAAGGTTTGCCTTCGACAACATTTGCCGAATCGGGTTTGGCTACGACCCTAAGTATCTCCTTCCTTCTCTGCCGCAAACTAAGTTCGCGGTGGCGTTTGACGATGCGGTCCAGATCAGCAGCGAGAGGTTCGGCTTCCTGAGACCGGTGTGGAAAATCAAAAGAGCTCTTGGAATCGGCTCGGAAAGCCGTATCAGCAGAGTGGTCTCCGATGTGCGTGAGTTCGCCAACACCATTATGAAGACCCTGGAGTCTCACGATCTGCTGTCTCGATTCTTGGGCTCCGGCCACTCCGACGAGAAGTTCGTCACCGACATCGTCATCAGCTTCACCCTCGCCGGAAGAGACACGACCTCAGCTGCTCTGACCTGGTTCTTCTGGCTAATCTCAAACCACCCAAACGTCGAGAGCGAAATTCTCAAGGAGATAGAAGAGAAATCGGAGAAGGGAGCCGGATATGATGAAGCGAAGGAGATGGTGTACATACACGCGGCGCTCTGCGAGAGCATGAGGCTGTACCCGCCGGTTCCGATCGACGGCAAAGAGGCGGTGAACGACGACGTTTTGCCTGACGGGACTAAAGTGAAGAAGGGGATGGCAGTGTCGTACCATGTTTACGCGATGGGGAGGATGGAGCAGATATGGGGCAAGGACTGGGCAGAGTTCCGACCAGAGAGGTGGTTGGAGTGTGACGTGGCGGCGGACAAGTGGAGGTTCGTGGCGAAGGACTCGTTTAGCTACCCGATGTTTCAGACGGGGCCGAGGATCTGTTTGGGGAAGGAGATGGCGTTCTTGCAGATGAAGAGGGTGGTTGTTGGGGTTTTGAAGAGGTTTAAGGTGGTGCCGGAGAAGAGAGAGAATGGATTAGAGCCGGAGTTTGTTGCTTACCTTACAGGGAAAATGAAGGGTGGGTTTCCGGTCACCGTTGTGGAGAGGGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

462

Amino Acids

52.5

Weight (kDa)

8.15

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 89 - 434 2.5e-43 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 554, 697
AccB1I GGYRCC 1 cut(s) 1288
AccII CGCG 3 cut(s) 536, 938, 1067
AciI CCGC 9 cut(s) 240, 521, 536, 554, 938, 968, 995, 1145, 1148
AclWI GGATC 1 cut(s) 1220
AcoI YGGCCR 3 cut(s) 192, 220, 733
AcsI RAATTY 3 cut(s) 320, 344, 861
AfaI GTAC 4 cut(s) 390, 929, 965, 1055
AfiI CCNNNNNNNGG 5 cut(s) 83, 119, 253, 592, 983
AgeI ACCGGT 1 cut(s) 592
AgsI TTSAA 5 cut(s) 260, 325, 353, 371, 1273
AjiI CACGTC 2 cut(s) 358, 1141
AjnI CCWGG 2 cut(s) 693, 815
AjuI GAANNNNNNNTTGG 2 cut(s) 106, 138
AleI CACNNNNGTG 1 cut(s) 1373
AloI GAACNNNNNNTCC 2 cut(s) 1220, 1252
AluBI AGCT 6 cut(s) 13, 97, 614, 774, 806, 1185
AluI AGCT 6 cut(s) 13, 97, 614, 774, 806, 1185
Alw21I GWGCWC 1 cut(s) 616
Alw26I GTCTC 5 cut(s) 583, 658, 705, 720, 786
AlwI GGATC 1 cut(s) 1220
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 4 cut(s) 192, 220, 733, 1205
ApeKI GCWGC 2 cut(s) 567, 806
ApoI RAATTY 3 cut(s) 320, 344, 861
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AsiGI ACCGGT 1 cut(s) 592
AspLEI GCGC 1 cut(s) 943
AspS9I GGNCC 2 cut(s) 556, 1205
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 7 cut(s) 200, 229, 311, 748, 769, 1009, 1360
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 556
BanI GGYRCC 1 cut(s) 1288
BanII GRGCYC 2 cut(s) 616, 731
BbsI GAAGAC 2 cut(s) 360, 695
Bbv12I GWGCWC 1 cut(s) 616
BbvCI CCTCAGC 1 cut(s) 802
BbvI GCAGC 2 cut(s) 579, 793
BccI CCATC 5 cut(s) 916, 1036, 1063, 1072, 1225
BceAI ACGGC 2 cut(s) 621, 1000
BciT130I CCWGG 2 cut(s) 695, 817
BcnI CCSGG 1 cut(s) 77
BcoDI GTCTC 5 cut(s) 583, 658, 705, 720, 786
BfaI CTAG 2 cut(s) 17, 411
BfoI RGCGCY 1 cut(s) 944
BisI GCNGC 5 cut(s) 521, 568, 807, 939, 1146
BlsI GCNGC 5 cut(s) 522, 569, 808, 940, 1147
Bme1390I CCNGG 3 cut(s) 77, 695, 817
Bme18I GGWCC 1 cut(s) 556
BmeT110I CYCGRG 1 cut(s) 8
BmgBI CACGTC 2 cut(s) 358, 1141
BmgT120I GGNCC 2 cut(s) 556, 1205
BmiI GGNNCC 5 cut(s) 730, 898, 975, 1206, 1290
BmrFI CCNGG 3 cut(s) 77, 695, 817
BmrI ACTGGG 1 cut(s) 1111
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 1111
BpiI GAAGAC 2 cut(s) 360, 695
BplI GAGNNNNNCTC 2 cut(s) 293, 325
BpmI CTGGAG 3 cut(s) 161, 295, 716
Bpu10I CCTNAGC 2 cut(s) 22, 802
BpuEI CTTGAG 3 cut(s) 44, 254, 851
BpuMI CCSGG 1 cut(s) 77
BsaI GGTCTC 2 cut(s) 583, 658
BsaJI CCNNGG 3 cut(s) 189, 693, 1208
BsaWI WCCGGW 2 cut(s) 592, 1363
BsaXI ACNNNNNCTCC 4 cut(s) 911, 941, 1220, 1250
Bsc4I CCNNNNNNNGG 5 cut(s) 83, 119, 253, 592, 983
Bse118I RCCGGY 3 cut(s) 83, 592, 970
Bse1I ACTGG 1 cut(s) 1106
BseBI CCWGG 2 cut(s) 695, 817
BseDI CCNNGG 3 cut(s) 189, 693, 1208
BseGI GGATG 3 cut(s) 264, 1047, 1083
BseLI CCNNNNNNNGG 5 cut(s) 83, 119, 253, 592, 983
BseMII CTCAG 3 cut(s) 329, 578, 816
BseNI ACTGG 1 cut(s) 1106
BseXI GCAGC 2 cut(s) 579, 793
BseYI CCCAGC 1 cut(s) 130
Bsh1236I CGCG 3 cut(s) 536, 938, 1067
Bsh1285I CGRYCG 1 cut(s) 981
BshFI GGCC 4 cut(s) 194, 222, 735, 1207
BshNI GGYRCC 1 cut(s) 1288
BshTI ACCGGT 1 cut(s) 592
BsiEI CGRYCG 1 cut(s) 981
BsiHKAI GWGCWC 1 cut(s) 616
BsiHKCI CYCGRG 1 cut(s) 8
BslFI GGGAC 1 cut(s) 1036
BslI CCNNNNNNNGG 5 cut(s) 83, 119, 253, 592, 983
BsmAI GTCTC 5 cut(s) 583, 658, 705, 720, 786
BsmFI GGGAC 1 cut(s) 1036
BsnI GGCC 4 cut(s) 194, 222, 735, 1207
Bso31I GGTCTC 2 cut(s) 583, 658
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 616, 731
Bsp1407I TGTACA 1 cut(s) 927
Bsp143I GATC 4 cut(s) 561, 706, 978, 1212
BspACI CCGC 9 cut(s) 240, 521, 536, 554, 938, 968, 995, 1145, 1148
BspANI GGCC 4 cut(s) 194, 222, 735, 1207
BspCNI CTCAG 3 cut(s) 330, 579, 815
BspFNI CGCG 3 cut(s) 536, 938, 1067
BspLI GGNNCC 5 cut(s) 730, 898, 975, 1206, 1290
BspPI GGATC 1 cut(s) 1220
BspT107I GGYRCC 1 cut(s) 1288
BspTNI GGTCTC 2 cut(s) 583, 658
BsrFI RCCGGY 3 cut(s) 83, 592, 970
BsrGI TGTACA 1 cut(s) 927
BsrI ACTGG 1 cut(s) 1106
BssAI RCCGGY 3 cut(s) 83, 592, 970
BssECI CCNNGG 3 cut(s) 189, 693, 1208
BssMI GATC 4 cut(s) 561, 706, 978, 1212
Bst2UI CCWGG 2 cut(s) 695, 817
Bst4CI ACNGT 2 cut(s) 253, 1372
Bst6I CTCTTC 5 cut(s) 784, 873, 1244, 1268, 1292
BstAUI TGTACA 1 cut(s) 927
BstC8I GCNNGC 2 cut(s) 95, 416
BstDEI CTNAG 6 cut(s) 22, 338, 498, 528, 587, 802
BstEII GGTNACC 1 cut(s) 1366
BstF5I GGATG 3 cut(s) 264, 1047, 1083
BstFNI CGCG 3 cut(s) 536, 938, 1067
BstH2I RGCGCY 1 cut(s) 944
BstHHI GCGC 1 cut(s) 943
BstKTI GATC 4 cut(s) 564, 709, 981, 1215
BstMAI GTCTC 5 cut(s) 583, 658, 705, 720, 786
BstMBI GATC 4 cut(s) 561, 706, 978, 1212
BstMCI CGRYCG 1 cut(s) 981
BstMWI GCNNNNNNNGC 4 cut(s) 43, 633, 642, 967
BstNI CCWGG 2 cut(s) 695, 817
BstPI GGTNACC 1 cut(s) 1366
BstSCI CCNGG 3 cut(s) 75, 693, 815
BstUI CGCG 3 cut(s) 536, 938, 1067
BstV1I GCAGC 2 cut(s) 579, 793
BstV2I GAAGAC 2 cut(s) 360, 695
BstX2I RGATCY 1 cut(s) 1212
BstXI CCANNNNNNTGG 1 cut(s) 1125
BstYI RGATCY 1 cut(s) 1212
BsuRI GGCC 4 cut(s) 194, 222, 735, 1207
BtgZI GCGATG 1 cut(s) 1082
BtrI CACGTC 2 cut(s) 358, 1141
BtsCI GGATG 3 cut(s) 264, 1047, 1083
BtsI GCAGTG 1 cut(s) 1053
BtsIMutI CAGTG 3 cut(s) 249, 335, 1053
Cac8I GCNNGC 2 cut(s) 95, 416
CfoI GCGC 1 cut(s) 943
Cfr10I RCCGGY 3 cut(s) 83, 592, 970
Cfr13I GGNCC 2 cut(s) 556, 1205
CsiI ACCWGGT 1 cut(s) 815
Csp6I GTAC 4 cut(s) 389, 928, 964, 1054
CspAI ACCGGT 1 cut(s) 592
CviAII CATG 4 cut(s) 149, 165, 955, 1058
CviQI GTAC 4 cut(s) 389, 928, 964, 1054
DdeI CTNAG 6 cut(s) 22, 338, 498, 528, 587, 802
DpnI GATC 4 cut(s) 563, 708, 980, 1214
DpnII GATC 4 cut(s) 561, 706, 978, 1212
DrdI GACNNNNNNGTC 2 cut(s) 554, 697
DseDI GACNNNNNNGTC 2 cut(s) 554, 697
EaeI YGGCCR 3 cut(s) 192, 220, 733
Eam1104I CTCTTC 5 cut(s) 784, 873, 1244, 1268, 1292
EarI CTCTTC 5 cut(s) 784, 873, 1244, 1268, 1292
EciI GGCGGA 1 cut(s) 1163
Ecl136II GAGCTC 1 cut(s) 614
Eco24I GRGCYC 2 cut(s) 616, 731
Eco31I GGTCTC 2 cut(s) 583, 658
Eco47I GGWCC 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 614
Eco88I CYCGRG 1 cut(s) 8
Eco91I GGTNACC 1 cut(s) 1366
EcoICRI GAGCTC 1 cut(s) 614
EcoO65I GGTNACC 1 cut(s) 1366
EcoRI GAATTC 1 cut(s) 320
EcoRII CCWGG 2 cut(s) 693, 815
EcoT38I GRGCYC 2 cut(s) 616, 731
FaeI CATG 4 cut(s) 152, 168, 958, 1061
FaiI YATR 9 cut(s) 109, 150, 166, 686, 906, 932, 956, 1059, 1090
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FaqI GGGAC 1 cut(s) 1036
FatI CATG 4 cut(s) 148, 164, 954, 1057
FauI CCCGC 1 cut(s) 975
Fnu4HI GCNGC 5 cut(s) 521, 568, 807, 939, 1146
FokI GGATG 3 cut(s) 251, 1054, 1090
FriOI GRGCYC 2 cut(s) 616, 731
Fsp4HI GCNGC 5 cut(s) 521, 568, 807, 939, 1146
FspBI CTAG 2 cut(s) 17, 411
GlaI GCGC 1 cut(s) 942
GluI GCNGC 5 cut(s) 521, 568, 807, 939, 1146
GsaI CCCAGC 1 cut(s) 134
GsuI CTGGAG 3 cut(s) 161, 295, 716
HaeII RGCGCY 1 cut(s) 944
HaeIII GGCC 4 cut(s) 194, 222, 735, 1207
HhaI GCGC 1 cut(s) 943
Hin1II CATG 4 cut(s) 152, 168, 958, 1061
Hin6I GCGC 1 cut(s) 941
HinP1I GCGC 1 cut(s) 941
HincII GTYRAC 1 cut(s) 364
HindII GTYRAC 1 cut(s) 364
HinfI GANTC 6 cut(s) 159, 477, 621, 698, 720, 1175
HphI GGTGA 7 cut(s) 200, 229, 311, 748, 769, 1009, 1360
Hpy166II GTNNAC 4 cut(s) 364, 928, 1000, 1063
Hpy188I TCNGA 8 cut(s) 631, 658, 742, 813, 889, 978, 1115, 1200
Hpy188III TCNNGA 9 cut(s) 8, 178, 271, 428, 559, 586, 704, 717, 853
Hpy8I GTNNAC 4 cut(s) 364, 928, 1000, 1063
Hpy99I CGWCG 6 cut(s) 236, 746, 854, 986, 1007, 1010
HpyAV CCTTC 9 cut(s) 469, 518, 522, 886, 910, 1030, 1165, 1219, 1345
HpyCH4III ACNGT 2 cut(s) 253, 1372
HpyCH4IV ACGT 4 cut(s) 357, 849, 1008, 1140
HpyCH4V TGCA 1 cut(s) 1243
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 633, 642, 967
HpyF3I CTNAG 6 cut(s) 22, 338, 498, 528, 587, 802
HpySE526I ACGT 4 cut(s) 357, 849, 1008, 1140
Hsp92II CATG 4 cut(s) 152, 168, 958, 1061
HspAI GCGC 1 cut(s) 941
Kzo9I GATC 4 cut(s) 561, 706, 978, 1212
LmnI GCTCC 5 cut(s) 42, 102, 734, 896, 1081
Lsp1109I GCAGC 2 cut(s) 579, 793
LweI GCATC 1 cut(s) 541
MabI ACCWGGT 1 cut(s) 815
MaeI CTAG 2 cut(s) 17, 411
MaeII ACGT 4 cut(s) 357, 849, 1008, 1140
MaeIII GTNAC 5 cut(s) 235, 333, 754, 1136, 1366
MalI GATC 4 cut(s) 563, 708, 980, 1214
MboI GATC 4 cut(s) 561, 706, 978, 1212
MflI RGATCY 1 cut(s) 1212
MhlI GDGCHC 2 cut(s) 616, 731
MluCI AATT 3 cut(s) 320, 344, 861
MlyI GAGTC 3 cut(s) 153, 707, 1169
MmeI TCCRAC 3 cut(s) 765, 1110, 1138
MseI TTAA 2 cut(s) 1281, 1387
MslI CAYNNNNRTG 2 cut(s) 683, 1373
MspA1I CMGCKG 1 cut(s) 806
MspR9I CCNGG 3 cut(s) 77, 695, 817
MvaI CCWGG 2 cut(s) 695, 817
MvnI CGCG 3 cut(s) 536, 938, 1067
MwoI GCNNNNNNNGC 4 cut(s) 43, 633, 642, 967
NciI CCSGG 1 cut(s) 77
NdeII GATC 4 cut(s) 561, 706, 978, 1212
NlaIII CATG 4 cut(s) 152, 168, 958, 1061
NlaIV GGNNCC 5 cut(s) 730, 898, 975, 1206, 1290
NmeAIII GCCGAG 2 cut(s) 170, 1233
NmuCI GTSAC 5 cut(s) 235, 333, 754, 1136, 1366
OliI CACNNNNGTG 1 cut(s) 1373
PaeR7I CTCGAG 1 cut(s) 8
PcsI WCGNNNNNNNCGW 4 cut(s) 354, 384, 750, 855
PfeI GAWTC 3 cut(s) 477, 621, 720
PflFI GACNNNGTC 1 cut(s) 764
PinAI ACCGGT 1 cut(s) 592
PkrI GCNGC 5 cut(s) 522, 569, 808, 940, 1147
Ple19I CGATCG 1 cut(s) 981
PleI GAGTC 3 cut(s) 153, 706, 1169
PpsI GAGTC 3 cut(s) 153, 706, 1169
Psp124BI GAGCTC 1 cut(s) 616
Psp6I CCWGG 2 cut(s) 693, 815
PspEI GGTNACC 1 cut(s) 1366
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 693, 815
PspN4I GGNNCC 5 cut(s) 730, 898, 975, 1206, 1290
PspPI GGNCC 2 cut(s) 556, 1205
PsuI RGATCY 1 cut(s) 1212
PsyI GACNNNGTC 1 cut(s) 764
PvuI CGATCG 1 cut(s) 981
PvuII CAGCTG 1 cut(s) 806
RsaI GTAC 4 cut(s) 390, 929, 965, 1055
RsaNI GTAC 4 cut(s) 389, 928, 964, 1054
RseI CAYNNNNRTG 2 cut(s) 683, 1373
SacI GAGCTC 1 cut(s) 616
SaqAI TTAA 2 cut(s) 1281, 1387
SatI GCNGC 5 cut(s) 521, 568, 807, 939, 1146
Sau3AI GATC 4 cut(s) 561, 706, 978, 1212
Sau96I GGNCC 2 cut(s) 556, 1205
SchI GAGTC 3 cut(s) 153, 707, 1169
ScrFI CCNGG 3 cut(s) 77, 695, 817
SduI GDGCHC 2 cut(s) 616, 731
SexAI ACCWGGT 1 cut(s) 815
SfaNI GCATC 1 cut(s) 541
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 556
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 2 cut(s) 683, 1373
SmlI CTYRAG 4 cut(s) 8, 59, 269, 866
SmoI CTYRAG 4 cut(s) 8, 59, 269, 866
Sse9I AATT 3 cut(s) 320, 344, 861
SsiI CCGC 9 cut(s) 240, 521, 536, 554, 938, 968, 995, 1145, 1148
SspMI CTAG 2 cut(s) 17, 411
SstI GAGCTC 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 75, 693, 815
TaaI ACNGT 2 cut(s) 253, 1372
TaiI ACGT 4 cut(s) 360, 852, 1011, 1143
TaqI TCGA 5 cut(s) 9, 462, 718, 852, 981
TasI AATT 3 cut(s) 320, 344, 861
TatI WGTACW 1 cut(s) 927
TauI GCSGC 3 cut(s) 523, 941, 1148
TfiI GAWTC 3 cut(s) 477, 621, 720
Tru1I TTAA 2 cut(s) 1281, 1387
Tru9I TTAA 2 cut(s) 1281, 1387
TscAI CASTG 3 cut(s) 256, 342, 1053
TseFI GTSAC 5 cut(s) 235, 333, 754, 1136, 1366
TseI GCWGC 2 cut(s) 567, 806
Tsp45I GTSAC 5 cut(s) 235, 333, 754, 1136, 1366
TspDTI ATGAA 5 cut(s) 114, 701, 924, 1262, 1364
TspGWI ACGGA 1 cut(s) 220
TspRI CASTG 3 cut(s) 256, 342, 1053
Tth111I GACNNNGTC 1 cut(s) 764
VpaK11BI GGWCC 1 cut(s) 556
XapI RAATTY 3 cut(s) 320, 344, 861
XcmI CCANNNNNNNNNTGG 1 cut(s) 250
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 2 cut(s) 17, 411
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.