Rorug06G0200600

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003462
Physical Location & Seq
Reverse (-)
32183587 .. 32185736
2150 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0200600.1

Sequence Viewer

Length: 585 bp
ATGTCTGGAGAGAAGCACCACCACGGCCTCTTCCACCACCACAAGGACAACGAGGATAAACCGGTTGAAACCTCCGCCTACAAATCACAGACTACTTACGAAGGCGGCGGCTACAGCGAGACCACCGGTTATTCCGCTGAGGGAGAGGGTGGCAGGTACAAAAGTGAGACCACCGACGCGTATGGCTCAGCTGCTCCCGGCGGTGGCCGATACACCAGCGAGACCACTGGTTACGTCAAGGAGGAACACGGTGGCAAGTACAGCGAGACCAGAACCGAAGCTTATGGCACCACTGAATCCGGAATCGATTACAAGAAGGAGGAGAAGCACCACAAACATCTCGAAGAACTCGGCGGGCTCGGTGCTGCTGCTGCTGGAGCTTATGCTTTGCATGAGAAGCATCAGGCAAAGAAAGACCCGGAGCATGCCCACAGGCACAAGATTGAAGAGGAGATCGCTGCAGTGGCTGCAGTTGGAGCCGGTGGGTTCGCCTTCCACGAGCACCATGAGAAGAAAGAGGCGAAGAAAGAGGCGAAGAAAGAAGAGGAAGAGGCTCATGGAAAGAAGCACCACCATCTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

194

Amino Acids

21.34

Weight (kDa)

6.18

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABA_WDS PF02496 103 - 179 6.7e-36 ABA/WDS induced protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 287
AccII CGCG 1 cut(s) 179
AccIII TCCGGA 1 cut(s) 299
AciI CCGC 6 cut(s) 75, 105, 108, 135, 201, 354
AcoI YGGCCR 1 cut(s) 205
AfaI GTAC 2 cut(s) 158, 260
AfiI CCNNNNNNNGG 2 cut(s) 43, 203
AflIII ACRYGT 1 cut(s) 177
AgeI ACCGGT 2 cut(s) 61, 125
AgsI TTSAA 2 cut(s) 68, 446
AluBI AGCT 3 cut(s) 191, 281, 380
AluI AGCT 3 cut(s) 191, 281, 380
Alw21I GWGCWC 1 cut(s) 504
Alw26I GTCTC 4 cut(s) 113, 161, 215, 260
AlwNI CAGNNNCTG 1 cut(s) 467
Aor13HI TCCGGA 1 cut(s) 299
AoxI GGCC 2 cut(s) 25, 205
ApeKI GCWGC 6 cut(s) 191, 365, 368, 371, 458, 467
AsiGI ACCGGT 2 cut(s) 61, 125
AsuC2I CCSGG 2 cut(s) 198, 419
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 360
BauI CACGAG 1 cut(s) 497
Bbv12I GWGCWC 1 cut(s) 504
BbvCI CCTCAGC 1 cut(s) 138
BbvI GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
BccI CCATC 1 cut(s) 582
BceAI ACGGC 1 cut(s) 40
BcnI CCSGG 2 cut(s) 198, 419
BcoDI GTCTC 4 cut(s) 113, 161, 215, 260
BfmI CTRYAG 3 cut(s) 112, 459, 468
BfuAI ACCTGC 1 cut(s) 144
BisI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
BlpI GCTNAGC 1 cut(s) 187
BlsI GCNGC 8 cut(s) 107, 110, 193, 367, 370, 373, 460, 469
Bme1390I CCNGG 2 cut(s) 198, 419
BmiI GGNNCC 2 cut(s) 289, 478
BmrFI CCNGG 2 cut(s) 198, 419
BmsI GCATC 1 cut(s) 409
BpmI CTGGAG 2 cut(s) 27, 396
Bpu10I CCTNAGC 1 cut(s) 138
Bpu1102I GCTNAGC 1 cut(s) 187
BpuMI CCSGG 2 cut(s) 198, 419
Bsa29I ATCGAT 1 cut(s) 306
BsaI GGTCTC 4 cut(s) 113, 161, 215, 260
BsaJI CCNNGG 1 cut(s) 22
BsaWI WCCGGW 3 cut(s) 61, 125, 299
Bsc4I CCNNNNNNNGG 2 cut(s) 43, 203
Bse118I RCCGGY 3 cut(s) 61, 125, 479
Bse1I ACTGG 1 cut(s) 232
BseAI TCCGGA 1 cut(s) 299
BseCI ATCGAT 1 cut(s) 306
BseDI CCNNGG 1 cut(s) 22
BseLI CCNNNNNNNGG 2 cut(s) 43, 203
BseMII CTCAG 2 cut(s) 129, 201
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 2 cut(s) 335, 464
BseXI GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
Bsh1236I CGCG 1 cut(s) 179
BshFI GGCC 2 cut(s) 27, 207
BshNI GGYRCC 1 cut(s) 287
BshTI ACCGGT 2 cut(s) 61, 125
BshVI ATCGAT 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 504
BsiSI CCGG 6 cut(s) 62, 126, 198, 300, 419, 480
BslI CCNNNNNNNGG 2 cut(s) 43, 203
BsmAI GTCTC 4 cut(s) 113, 161, 215, 260
BsnI GGCC 2 cut(s) 27, 207
Bso31I GGTCTC 4 cut(s) 113, 161, 215, 260
Bsp1286I GDGCHC 2 cut(s) 360, 504
Bsp13I TCCGGA 1 cut(s) 299
Bsp143I GATC 1 cut(s) 453
Bsp1720I GCTNAGC 1 cut(s) 187
BspACI CCGC 6 cut(s) 75, 105, 108, 135, 201, 354
BspANI GGCC 2 cut(s) 27, 207
BspCNI CTCAG 2 cut(s) 130, 200
BspDI ATCGAT 1 cut(s) 306
BspEI TCCGGA 1 cut(s) 299
BspFNI CGCG 1 cut(s) 179
BspLI GGNNCC 2 cut(s) 289, 478
BspMAI CTGCAG 2 cut(s) 463, 472
BspMI ACCTGC 1 cut(s) 144
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 4 cut(s) 113, 161, 215, 260
BsrFI RCCGGY 3 cut(s) 61, 125, 479
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 3 cut(s) 61, 125, 479
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 453
BssSI CACGAG 1 cut(s) 497
Bst2BI CACGAG 1 cut(s) 497
Bst4CI ACNGT 1 cut(s) 251
Bst6I CTCTTC 4 cut(s) 35, 441, 537, 543
BstAPI GCANNNNNTGC 1 cut(s) 467
BstC8I GCNNGC 2 cut(s) 356, 426
BstDEI CTNAG 2 cut(s) 138, 187
BstDSI CCRYGG 1 cut(s) 22
BstFNI CGCG 1 cut(s) 179
BstKTI GATC 1 cut(s) 456
BstMAI GTCTC 4 cut(s) 113, 161, 215, 260
BstMBI GATC 1 cut(s) 453
BstMWI GCNNNNNNNGC 8 cut(s) 114, 261, 371, 377, 397, 464, 467, 476
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 196, 417
BstSFI CTRYAG 3 cut(s) 112, 459, 468
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
Bsu15I ATCGAT 1 cut(s) 306
BsuRI GGCC 2 cut(s) 27, 207
BsuTUI ATCGAT 1 cut(s) 306
BtgI CCRYGG 1 cut(s) 22
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 3 cut(s) 225, 291, 468
BveI ACCTGC 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 356, 426
CaiI CAGNNNCTG 1 cut(s) 467
Cfr10I RCCGGY 3 cut(s) 61, 125, 479
ClaI ATCGAT 1 cut(s) 306
CseI GACGC 1 cut(s) 185
Csp6I GTAC 2 cut(s) 157, 259
CspAI ACCGGT 2 cut(s) 61, 125
CviAII CATG 4 cut(s) 392, 425, 506, 557
CviQI GTAC 2 cut(s) 157, 259
DdeI CTNAG 2 cut(s) 138, 187
DpnI GATC 1 cut(s) 455
DpnII GATC 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 4 cut(s) 35, 441, 537, 543
EarI CTCTTC 4 cut(s) 35, 441, 537, 543
EciI GGCGGA 1 cut(s) 64
Eco24I GRGCYC 1 cut(s) 360
Eco31I GGTCTC 4 cut(s) 113, 161, 215, 260
EcoT38I GRGCYC 1 cut(s) 360
FaeI CATG 4 cut(s) 395, 428, 509, 560
FaiI YATR 7 cut(s) 183, 285, 384, 393, 426, 507, 558
FatI CATG 4 cut(s) 391, 424, 505, 556
FauI CCCGC 1 cut(s) 347
Fnu4HI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
FriOI GRGCYC 1 cut(s) 360
Fsp4HI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
GluI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
GsuI CTGGAG 2 cut(s) 27, 396
HaeIII GGCC 2 cut(s) 27, 207
HapII CCGG 6 cut(s) 62, 126, 198, 300, 419, 480
HgaI GACGC 1 cut(s) 185
Hin1II CATG 4 cut(s) 395, 428, 509, 560
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 2 cut(s) 296, 303
HpaII CCGG 6 cut(s) 62, 126, 198, 300, 419, 480
Hpy188III TCNNGA 3 cut(s) 6, 300, 341
Hpy99I CGWCG 1 cut(s) 179
HpyAV CCTTC 3 cut(s) 95, 310, 502
HpyCH4III ACNGT 1 cut(s) 251
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 3 cut(s) 391, 461, 470
HpyF10VI GCNNNNNNNGC 8 cut(s) 114, 261, 371, 377, 397, 464, 467, 476
HpyF3I CTNAG 2 cut(s) 138, 187
HpySE526I ACGT 1 cut(s) 234
Hsp92II CATG 4 cut(s) 395, 428, 509, 560
Kpn2I TCCGGA 1 cut(s) 299
Kzo9I GATC 1 cut(s) 453
LmnI GCTCC 4 cut(s) 199, 377, 421, 476
Lsp1109I GCAGC 6 cut(s) 178, 352, 355, 358, 445, 454
LweI GCATC 1 cut(s) 409
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 230
MalI GATC 1 cut(s) 455
MboI GATC 1 cut(s) 453
MboII GAAGA 9 cut(s) 22, 356, 458, 523, 535, 547, 554, 560, 571
MhlI GDGCHC 2 cut(s) 360, 504
MluI ACGCGT 1 cut(s) 177
MmeI TCCRAC 1 cut(s) 454
MroI TCCGGA 1 cut(s) 299
MspA1I CMGCKG 2 cut(s) 137, 191
MspI CCGG 6 cut(s) 62, 126, 198, 300, 419, 480
MspR9I CCNGG 2 cut(s) 198, 419
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 8 cut(s) 114, 261, 371, 377, 397, 464, 467, 476
NciI CCSGG 2 cut(s) 198, 419
NdeII GATC 1 cut(s) 453
NlaIII CATG 4 cut(s) 395, 428, 509, 560
NlaIV GGNNCC 2 cut(s) 289, 478
NmeAIII GCCGAG 1 cut(s) 330
NspI RCATGY 1 cut(s) 428
PaeI GCATGC 1 cut(s) 428
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PfeI GAWTC 2 cut(s) 296, 303
PinAI ACCGGT 2 cut(s) 61, 125
PkrI GCNGC 8 cut(s) 107, 110, 193, 367, 370, 373, 460, 469
PspN4I GGNNCC 2 cut(s) 289, 478
PstI CTGCAG 2 cut(s) 463, 472
PstNI CAGNNNCTG 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 191
RsaI GTAC 2 cut(s) 158, 260
RsaNI GTAC 2 cut(s) 157, 259
SatI GCNGC 8 cut(s) 106, 109, 192, 366, 369, 372, 459, 468
Sau3AI GATC 1 cut(s) 453
ScrFI CCNGG 2 cut(s) 198, 419
SduI GDGCHC 2 cut(s) 360, 504
SetI ASST 6 cut(s) 74, 158, 193, 237, 283, 382
SfaNI GCATC 1 cut(s) 409
SfcI CTRYAG 3 cut(s) 112, 459, 468
SphI GCATGC 1 cut(s) 428
SsiI CCGC 6 cut(s) 75, 105, 108, 135, 201, 354
StyD4I CCNGG 2 cut(s) 196, 417
TaaI ACNGT 1 cut(s) 251
TaiI ACGT 1 cut(s) 237
TaqI TCGA 2 cut(s) 306, 342
TatI WGTACW 1 cut(s) 258
TauI GCSGC 2 cut(s) 108, 111
TfiI GAWTC 2 cut(s) 296, 303
TscAI CASTG 3 cut(s) 232, 298, 468
TseI GCWGC 6 cut(s) 191, 365, 368, 371, 458, 467
TspRI CASTG 3 cut(s) 232, 298, 468
XceI RCATGY 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.