Rroxscaffold_7G00179560

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003462
Physical Location & Seq
Forward (+)
19091746 .. 19092927
1182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00179560.1

Sequence Viewer

Length: 1146 bp
ATGTTTCTCGAGCTGTTAGCCTTCTCCTCCCTTCTCCTCATCCTCCCCTTGCTTTTCTTCATCCTCAAGCTTTCCCGCAACTCGAAAAACAACTGCAACAGCAAGCTCCCCAAATCATACCCAATAATCGGTTCATTCCCAGCCCTCTATAAGAACTATGCCAATAGACTCCCATGGTTCACTGAACTTCTCCAAGACTCGCCCACATCTACTATTACCATGCACCATTCTCTGGGCCGCCACATCGTCGTCACCGCCAACTCGGCCGTGGTCCAGCACATCCTCAAGATAAAGTTCCACATCTACCAAATGGGCGAGTCCTTCCGTACAACTCTCCACGACCTCCTTGGCGATGGGATTTTCAACGCCGACGGTGACAATTGGAAGTTCCAGCGGCAACTCTCCAGCCATGAATTCAACACAAAGTCCCTCAGGAAATTTGTGGAAGACGTGGTGGACACTGAGCTCTACGATCAGCTGATCCCTATTCTATCCAAATCTTCCAGCGACCGAACCATTCTTGATTTCCAAGACATTCTCCAGCGGTTCACCTTCGACAACATTTGCCGGATCGGGTTCGGGTACGACCTGGAGTATCTCCTTCCTTCTCTGCCTCAAGCTAAATTCACGGTGGAGTTCGACAATGCGGTCCAGATCAGCAGCGAAAGGTTCAGTTCCATGCGCCCGGAAAGCCATCTCCGCTGTGTGGTCTCTGACGTCCGAGAGTTTGCGAACACCATCGTCAAGCAAAAGAAGCATGAAATGGACGAGAAGAAGACTCTGGAGTTTCACGATCTGCTGTCTAGGTTCTTGAGCTCCGCCCACTCCGATGAGAAATTCGTCACTGACATCGTCATCAGCTTCACTCTCGCCGGAAGGGACACGACCTCGGCGGCTTTGACCTGGTTCTTCTGGCTAATGTCAAACCATCCCAACGTCGAAAACCAAATTCTCAAGGAGATCAATGAGAAAACGAAGACCGGAGCCAGATATGATGAAGCGAAGGCGATGGTGTACACACACGCGGCGCTCTGTGAGAGCATGAGGCTGTACCCACCGGTTCCGACTGACAACAAAGAGGCGGTAGAAGACGACATTTTGCCGGACGGGACTAAAGTGAAGAATGGGAGTATGGGACGACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00073 map00073
Pfam Domains
Protein Families
p450

Protein Analysis

381

Amino Acids

43.75

Weight (kDa)

6.36

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 37 - 377 3.6e-37 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 647
AatII GACGTC 1 cut(s) 720
AccB7I CCANNNNNTGG 1 cut(s) 232
AccII CGCG 1 cut(s) 1025
AclWI GGATC 2 cut(s) 475, 578
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 5 cut(s) 413, 437, 623, 836, 948
AcyI GRCGYC 1 cut(s) 717
AfaI GTAC 4 cut(s) 328, 584, 1016, 1052
AfiI CCNNNNNNNGG 3 cut(s) 128, 232, 706
AgeI ACCGGT 1 cut(s) 1057
AgsI TTSAA 2 cut(s) 364, 418
AjiI CACGTC 1 cut(s) 451
AjnI CCWGG 2 cut(s) 588, 902
AjuI GAANNNNNNNTTGG 2 cut(s) 115, 147
AluBI AGCT 8 cut(s) 13, 70, 106, 466, 478, 620, 816, 861
AluI AGCT 8 cut(s) 13, 70, 106, 466, 478, 620, 816, 861
Alw21I GWGCWC 2 cut(s) 468, 818
Alw26I GTCTC 1 cut(s) 715
AlwI GGATC 2 cut(s) 475, 578
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 2 cut(s) 235, 264
ApeKI GCWGC 1 cut(s) 660
ApoI RAATTY 5 cut(s) 413, 437, 623, 836, 948
AsiGI ACCGGT 1 cut(s) 1057
AspLEI GCGC 2 cut(s) 684, 1030
AspS9I GGNCC 3 cut(s) 235, 271, 649
AsuC2I CCSGG 1 cut(s) 686
AsuHPI GGTGA 3 cut(s) 244, 386, 541
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 2 cut(s) 271, 649
AxyI CCTNAGG 1 cut(s) 431
BanII GRGCYC 2 cut(s) 468, 818
BbsI GAAGAC 4 cut(s) 453, 782, 983, 1095
Bbv12I GWGCWC 2 cut(s) 468, 818
BbvI GCAGC 1 cut(s) 672
BccI CCATC 5 cut(s) 347, 702, 746, 936, 1003
BceAI ACGGC 1 cut(s) 251
BciT130I CCWGG 2 cut(s) 590, 904
BcnI CCSGG 1 cut(s) 686
BcoDI GTCTC 1 cut(s) 715
BfaI CTAG 1 cut(s) 804
BfoI RGCGCY 1 cut(s) 1031
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 5 cut(s) 238, 395, 661, 894, 1026
BlsI GCNGC 5 cut(s) 239, 396, 662, 895, 1027
Bme1390I CCNGG 3 cut(s) 590, 686, 904
Bme18I GGWCC 2 cut(s) 271, 649
BmeT110I CYCGRG 1 cut(s) 8
BmgBI CACGTC 1 cut(s) 451
BmgT120I GGNCC 3 cut(s) 235, 271, 649
BmiI GGNNCC 2 cut(s) 985, 1062
BmrFI CCNGG 3 cut(s) 590, 686, 904
BpiI GAAGAC 4 cut(s) 453, 782, 983, 1095
BpmI CTGGAG 4 cut(s) 388, 524, 611, 803
BpuEI CTTGAG 5 cut(s) 50, 269, 600, 832, 938
BpuMI CCSGG 1 cut(s) 686
BsaHI GRCGYC 1 cut(s) 717
BsaI GGTCTC 1 cut(s) 715
BsaJI CCNNGG 4 cut(s) 173, 267, 346, 888
BsaWI WCCGGW 2 cut(s) 980, 1057
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 232, 706
Bse118I RCCGGY 1 cut(s) 1057
Bse21I CCTNAGG 1 cut(s) 431
BseBI CCWGG 2 cut(s) 590, 904
BseDI CCNNGG 4 cut(s) 173, 267, 346, 888
BseGI GGATG 4 cut(s) 39, 60, 279, 928
BseLI CCNNNNNNNGG 3 cut(s) 128, 232, 706
BseMII CTCAG 2 cut(s) 445, 453
BseRI GAGGAG 2 cut(s) 16, 26
BseX3I CGGCCG 1 cut(s) 264
BseXI GCAGC 1 cut(s) 672
BseYI CCCAGC 1 cut(s) 139
Bsh1236I CGCG 1 cut(s) 1025
Bsh1285I CGRYCG 2 cut(s) 267, 511
BshFI GGCC 2 cut(s) 237, 266
BshTI ACCGGT 1 cut(s) 1057
BsiEI CGRYCG 2 cut(s) 267, 511
BsiHKAI GWGCWC 2 cut(s) 468, 818
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 6 cut(s) 568, 686, 873, 981, 1058, 1103
BslFI GGGAC 3 cut(s) 412, 893, 1123
BslI CCNNNNNNNGG 3 cut(s) 128, 232, 706
BsmAI GTCTC 1 cut(s) 715
BsmFI GGGAC 3 cut(s) 412, 893, 1123
BsnI GGCC 2 cut(s) 237, 266
Bso31I GGTCTC 1 cut(s) 715
BsoBI CYCGRG 1 cut(s) 8
Bsp1286I GDGCHC 2 cut(s) 468, 818
Bsp1407I TGTACA 1 cut(s) 1014
Bsp143I GATC 6 cut(s) 472, 480, 570, 654, 793, 960
Bsp19I CCATGG 1 cut(s) 173
BspANI GGCC 2 cut(s) 237, 266
BspCNI CTCAG 2 cut(s) 444, 454
BspFNI CGCG 1 cut(s) 1025
BspLI GGNNCC 2 cut(s) 985, 1062
BspPI GGATC 2 cut(s) 475, 578
BspTNI GGTCTC 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 1057
BsrGI TGTACA 1 cut(s) 1014
BssAI RCCGGY 1 cut(s) 1057
BssECI CCNNGG 4 cut(s) 173, 267, 346, 888
BssMI GATC 6 cut(s) 472, 480, 570, 654, 793, 960
BssNI GRCGYC 1 cut(s) 717
BssT1I CCWWGG 2 cut(s) 173, 346
Bst2UI CCWGG 2 cut(s) 590, 904
Bst4CI ACNGT 3 cut(s) 374, 631, 1143
BstACI GRCGYC 1 cut(s) 717
BstAUI TGTACA 1 cut(s) 1014
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 2 cut(s) 431, 462
BstDSI CCRYGG 2 cut(s) 173, 267
BstF5I GGATG 4 cut(s) 39, 60, 279, 928
BstFNI CGCG 1 cut(s) 1025
BstH2I RGCGCY 1 cut(s) 1031
BstHHI GCGC 2 cut(s) 684, 1030
BstKTI GATC 6 cut(s) 475, 483, 573, 657, 796, 963
BstMAI GTCTC 1 cut(s) 715
BstMBI GATC 6 cut(s) 472, 480, 570, 654, 793, 960
BstMCI CGRYCG 2 cut(s) 267, 511
BstMWI GCNNNNNNNGC 4 cut(s) 263, 690, 699, 754
BstNI CCWGG 2 cut(s) 590, 904
BstSCI CCNGG 3 cut(s) 588, 684, 902
BstUI CGCG 1 cut(s) 1025
BstV1I GCAGC 1 cut(s) 672
BstV2I GAAGAC 4 cut(s) 453, 782, 983, 1095
BstZI CGGCCG 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 431
BsuRI GGCC 2 cut(s) 237, 266
BtgI CCRYGG 2 cut(s) 173, 267
BtgZI GCGATG 2 cut(s) 366, 1022
BtrI CACGTC 1 cut(s) 451
BtsCI GGATG 4 cut(s) 39, 60, 279, 928
BtsIMutI CAGTG 3 cut(s) 180, 459, 843
Cac8I GCNNGC 1 cut(s) 104
CfoI GCGC 2 cut(s) 684, 1030
Cfr10I RCCGGY 1 cut(s) 1057
Cfr13I GGNCC 3 cut(s) 235, 271, 649
CsiI ACCWGGT 1 cut(s) 902
Csp6I GTAC 4 cut(s) 327, 583, 1015, 1051
CspAI ACCGGT 1 cut(s) 1057
CviAII CATG 6 cut(s) 174, 220, 410, 679, 758, 1042
CviQI GTAC 4 cut(s) 327, 583, 1015, 1051
DdeI CTNAG 2 cut(s) 431, 462
DpnI GATC 6 cut(s) 474, 482, 572, 656, 795, 962
DpnII GATC 6 cut(s) 472, 480, 570, 654, 793, 960
DrdI GACNNNNNNGTC 1 cut(s) 647
DseDI GACNNNNNNGTC 1 cut(s) 647
EaeI YGGCCR 1 cut(s) 264
EagI CGGCCG 1 cut(s) 264
EciI GGCGGA 1 cut(s) 808
Ecl136II GAGCTC 2 cut(s) 466, 816
EclXI CGGCCG 1 cut(s) 264
Eco130I CCWWGG 2 cut(s) 173, 346
Eco24I GRGCYC 2 cut(s) 468, 818
Eco31I GGTCTC 1 cut(s) 715
Eco47I GGWCC 2 cut(s) 271, 649
Eco52I CGGCCG 1 cut(s) 264
Eco53kI GAGCTC 2 cut(s) 466, 816
Eco81I CCTNAGG 1 cut(s) 431
Eco88I CYCGRG 1 cut(s) 8
EcoICRI GAGCTC 2 cut(s) 466, 816
EcoRI GAATTC 1 cut(s) 413
EcoRII CCWGG 2 cut(s) 588, 902
EcoT14I CCWWGG 2 cut(s) 173, 346
EcoT38I GRGCYC 2 cut(s) 468, 818
ErhI CCWWGG 2 cut(s) 173, 346
FaeI CATG 6 cut(s) 177, 223, 413, 682, 761, 1045
FaqI GGGAC 3 cut(s) 412, 893, 1123
FatI CATG 6 cut(s) 173, 219, 409, 678, 757, 1041
FauI CCCGC 1 cut(s) 83
Fnu4HI GCNGC 5 cut(s) 238, 395, 661, 894, 1026
FokI GGATG 4 cut(s) 26, 47, 266, 915
FriOI GRGCYC 2 cut(s) 468, 818
Fsp4HI GCNGC 5 cut(s) 238, 395, 661, 894, 1026
FspBI CTAG 1 cut(s) 804
GlaI GCGC 2 cut(s) 683, 1029
GluI GCNGC 5 cut(s) 238, 395, 661, 894, 1026
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 4 cut(s) 388, 524, 611, 803
HaeII RGCGCY 1 cut(s) 1031
HaeIII GGCC 2 cut(s) 237, 266
HapII CCGG 6 cut(s) 568, 686, 873, 981, 1058, 1103
HhaI GCGC 2 cut(s) 684, 1030
Hin1I GRCGYC 1 cut(s) 717
Hin1II CATG 6 cut(s) 177, 223, 413, 682, 761, 1045
Hin6I GCGC 2 cut(s) 682, 1028
HinP1I GCGC 2 cut(s) 682, 1028
HindIII AAGCTT 1 cut(s) 68
HinfI GANTC 4 cut(s) 168, 197, 317, 778
HpaII CCGG 6 cut(s) 568, 686, 873, 981, 1058, 1103
HphI GGTGA 3 cut(s) 244, 386, 541
Hpy166II GTNNAC 5 cut(s) 180, 457, 549, 1015, 1017
Hpy188I TCNGA 4 cut(s) 715, 722, 829, 1065
Hpy188III TCNNGA 8 cut(s) 8, 286, 433, 521, 652, 782, 791, 811
Hpy8I GTNNAC 5 cut(s) 180, 457, 549, 1015, 1017
Hpy99I CGWCG 3 cut(s) 251, 374, 941
HpyAV CCTTC 8 cut(s) 31, 41, 331, 562, 611, 615, 870, 997
HpyCH4III ACNGT 3 cut(s) 374, 631, 1143
HpyCH4IV ACGT 3 cut(s) 450, 717, 936
HpyCH4V TGCA 2 cut(s) 96, 223
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 690, 699, 754
HpyF3I CTNAG 2 cut(s) 431, 462
HpySE526I ACGT 3 cut(s) 450, 717, 936
Hsp92I GRCGYC 1 cut(s) 717
Hsp92II CATG 6 cut(s) 177, 223, 413, 682, 761, 1045
HspAI GCGC 2 cut(s) 682, 1028
Kzo9I GATC 6 cut(s) 472, 480, 570, 654, 793, 960
LmnI GCTCC 3 cut(s) 111, 821, 983
Lsp1109I GCAGC 1 cut(s) 672
MabI ACCWGGT 1 cut(s) 902
MaeI CTAG 1 cut(s) 804
MaeII ACGT 3 cut(s) 450, 717, 936
MaeIII GTNAC 3 cut(s) 250, 374, 841
MalI GATC 6 cut(s) 474, 482, 572, 656, 795, 962
MboI GATC 6 cut(s) 472, 480, 570, 654, 793, 960
MboII GAAGA 9 cut(s) 49, 458, 492, 784, 787, 901, 988, 1100, 1132
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 2 cut(s) 468, 818
MluCI AATT 6 cut(s) 379, 413, 437, 623, 836, 948
MlyI GAGTC 4 cut(s) 162, 191, 326, 772
MmeI TCCRAC 1 cut(s) 1088
MslI CAYNNNNRTG 1 cut(s) 828
MspA1I CMGCKG 4 cut(s) 394, 478, 544, 702
MspI CCGG 6 cut(s) 568, 686, 873, 981, 1058, 1103
MspR9I CCNGG 3 cut(s) 590, 686, 904
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 2 cut(s) 590, 904
MvnI CGCG 1 cut(s) 1025
MwoI GCNNNNNNNGC 4 cut(s) 263, 690, 699, 754
NciI CCSGG 1 cut(s) 686
NcoI CCATGG 1 cut(s) 173
NdeII GATC 6 cut(s) 472, 480, 570, 654, 793, 960
NlaIII CATG 6 cut(s) 177, 223, 413, 682, 761, 1045
NlaIV GGNNCC 2 cut(s) 985, 1062
NmeAIII GCCGAG 2 cut(s) 242, 869
NmuCI GTSAC 3 cut(s) 250, 374, 841
PaeR7I CTCGAG 1 cut(s) 8
PflFI GACNNNGTC 1 cut(s) 851
PflMI CCANNNNNTGG 1 cut(s) 232
PinAI ACCGGT 1 cut(s) 1057
PkrI GCNGC 5 cut(s) 239, 396, 662, 895, 1027
PleI GAGTC 4 cut(s) 162, 191, 325, 772
PpsI GAGTC 4 cut(s) 162, 191, 325, 772
Psp124BI GAGCTC 2 cut(s) 468, 818
Psp6I CCWGG 2 cut(s) 588, 902
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 2 cut(s) 588, 902
PspN4I GGNNCC 2 cut(s) 985, 1062
PspPI GGNCC 3 cut(s) 235, 271, 649
PsyI GACNNNGTC 1 cut(s) 851
PvuII CAGCTG 1 cut(s) 478
RsaI GTAC 4 cut(s) 328, 584, 1016, 1052
RsaNI GTAC 4 cut(s) 327, 583, 1015, 1051
RseI CAYNNNNRTG 1 cut(s) 828
SacI GAGCTC 2 cut(s) 468, 818
SatI GCNGC 5 cut(s) 238, 395, 661, 894, 1026
Sau3AI GATC 6 cut(s) 472, 480, 570, 654, 793, 960
Sau96I GGNCC 3 cut(s) 235, 271, 649
SchI GAGTC 4 cut(s) 162, 191, 326, 772
ScrFI CCNGG 3 cut(s) 590, 686, 904
SduI GDGCHC 2 cut(s) 468, 818
SexAI ACCWGGT 1 cut(s) 902
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 2 cut(s) 271, 649
SlaI CTCGAG 1 cut(s) 8
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 6 cut(s) 8, 65, 284, 615, 811, 953
SmoI CTYRAG 6 cut(s) 8, 65, 284, 615, 811, 953
Sse9I AATT 6 cut(s) 379, 413, 437, 623, 836, 948
SspMI CTAG 1 cut(s) 804
SstI GAGCTC 2 cut(s) 468, 818
StyD4I CCNGG 3 cut(s) 588, 684, 902
StyI CCWWGG 2 cut(s) 173, 346
TaaI ACNGT 3 cut(s) 374, 631, 1143
TaiI ACGT 3 cut(s) 453, 720, 939
TaqI TCGA 5 cut(s) 9, 83, 555, 639, 939
TaqII GACCGA 1 cut(s) 525
TasI AATT 6 cut(s) 379, 413, 437, 623, 836, 948
TatI WGTACW 1 cut(s) 1014
TauI GCSGC 4 cut(s) 240, 397, 896, 1028
TscAI CASTG 3 cut(s) 187, 466, 850
TseFI GTSAC 3 cut(s) 250, 374, 841
TseI GCWGC 1 cut(s) 660
Tsp45I GTSAC 3 cut(s) 250, 374, 841
TspDTI ATGAA 5 cut(s) 49, 123, 426, 774, 1011
TspGWI ACGGA 1 cut(s) 314
TspRI CASTG 3 cut(s) 187, 466, 850
Tth111I GACNNNGTC 1 cut(s) 851
Van91I CCANNNNNTGG 1 cut(s) 232
VpaK11BI GGWCC 2 cut(s) 271, 649
XapI RAATTY 5 cut(s) 413, 437, 623, 836, 948
XcmI CCANNNNNNNNNTGG 2 cut(s) 265, 344
XhoI CTCGAG 1 cut(s) 8
XspI CTAG 1 cut(s) 804
ZraI GACGTC 1 cut(s) 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.